Lsi07G014450 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi07G014450 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 4.6e-159
Identity = 313/679 (46.10%), Postives = 404/679 (59.50%), Query Frame = 0

Query: 119 RPFFAAPPPRKKIAKEAILYSYNKNINGFAAMLDEKQTADLA------------------ 178
           R F A+     + AKEAI YSY ++INGFAA+LDE + A++A                  
Sbjct: 68  RTFLASFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHT 127

Query: 179 -------------------------------------SVWPESKSFSDEGYGPVP----- 238
                                                 VWPESKSFSDEGYG VP     
Sbjct: 128 THSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKG 187

Query: 239 --------SRKLIGARFFNIGYGEL-----SVTFNSSRDNEGHGTHTLSIAGGNFVSGAN 298
                   +RKLIGAR+FN GY        + ++ + RD++GHG+HTLS A GNFV GAN
Sbjct: 188 RCHKDVPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGAN 247

Query: 299 VLGMGNGTIKGGSPRARVASYKVCWPEESG-ECLDPNTLAAFEAAIGDGVDVISISVGGE 358
           V G+GNGT  GGSP+ARVA+YKVCWP   G EC D + LAA EAAI DGVDV+S SVGG+
Sbjct: 248 VFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGD 307

Query: 359 PKEFFSDALSVGAFHAVERGIVVVCSAGNVGPTPGTVSNVSPWILTVGASSIDRDFTNFV 418
             ++ SD +++G+FHAV+ G+ VVCSAGN GP  GTVSNV+PW++TVGASS+DR+F  FV
Sbjct: 308 AGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFV 367

Query: 419 VLGSKKKLKGTSFSSKALPFNKFYPLINAVDAKANNVSNSDAEVCEEGSLDPKKLTGKIV 478
            L + +  KGTS  SK LP  K Y LI+A DA   N + +DA +C++GSLDPKK+ GKI+
Sbjct: 368 ELKNGQSFKGTSL-SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKIL 427

Query: 479 VCLRGGLPRVSKGYVAAKAGAVGMILVNDEESGNAILTDLHILPASH------------- 538
           VCLRG   RV KG  AA AGA GM+L ND+ SGN I++D H+LPAS              
Sbjct: 428 VCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYL 487

Query: 539 ------------------------------------------PDITAPGVNILAAYPDGV 598
                                                     PDITAPGVNI+AA+ +  
Sbjct: 488 SSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEAT 547

Query: 599 PLTELPLDDRQAPFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNN 658
             T+L  D+R+ PF  +SGTSM+CPH++G+VGLLKTL+P WSPAAI+SAIMTT++T +N 
Sbjct: 548 GPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNR 607

Query: 659 LHPILDFTGLIATPLAYGAGHVNPNSAMDPGLIYDITIDDYLNFLCARGYNATQIEKISK 668
             P++D +   A P +YG+GHV PN A  PGL+YD+T  DYL+FLCA GYN T ++  ++
Sbjct: 608 RKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAE 667

BLAST of Lsi07G014450 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 4.8e-156
Identity = 310/676 (45.86%), Postives = 396/676 (58.58%), Query Frame = 0

Query: 132 AKEAILYSYNKNINGFAAMLDEKQTADLA------------------------------- 191
           A +AI YSY K+INGFAA LD     +++                               
Sbjct: 71  ATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNS 130

Query: 192 ------------------------SVWPESKSFSDEGYGPVPS----------------- 251
                                    VWPESKSF DEG GP+PS                 
Sbjct: 131 YVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCN 190

Query: 252 RKLIGARFFNIGY----GELSVTFNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIK 311
           RKLIGAR+FN GY    G L+ +F+S RD +GHG+HTLS A G+FV G ++ G GNGT K
Sbjct: 191 RKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAK 250

Query: 312 GGSPRARVASYKVCWPEESG-ECLDPNTLAAFEAAIGDGVDVISISVGGEPKEFFSDALS 371
           GGSPRARVA+YKVCWP   G EC D + LAAF+AAI DG DVIS+S+GGEP  FF+D+++
Sbjct: 251 GGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVA 310

Query: 372 VGAFHAVERGIVVVCSAGNVGPTPGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKG 431
           +G+FHA ++ IVVVCSAGN GP   TVSNV+PW +TVGAS++DR+F + +VLG+ K  KG
Sbjct: 311 IGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKG 370

Query: 432 TSFSSKALPFNKFYPLINAVDAKANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGLPRV 491
            S SS ALP  KFYP++ +V+AKA N S  DA++C+ GSLDP K  GKI+VCLRG   RV
Sbjct: 371 QSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRV 430

Query: 492 SKGYVAAKAGAVGMILVNDEESGNAILTDLHILPASH----------------------- 551
            KG   A  G +GM+L N   +GN +L D H+LPA+                        
Sbjct: 431 EKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHI 490

Query: 552 --------------------------------PDITAPGVNILAAYPDGVPLTELPLDDR 611
                                           PDITAPGV+++AAY   V  T    D R
Sbjct: 491 TPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPR 550

Query: 612 QAPFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILDFTGL 671
           +  F   SGTSM+CPH++GI GLLKT  P WSPAAI+SAIMTTA   D+   PI + T +
Sbjct: 551 RLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNM 610

Query: 672 IATPLAYGAGHVNPNSAMDPGLIYDITIDDYLNFLCARGYNATQIEKISKKIFVCDR-SF 675
            ATP ++GAGHV PN A++PGL+YD+ I DYLNFLC+ GYNA+QI   S   F C     
Sbjct: 611 KATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKI 670

BLAST of Lsi07G014450 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 456.1 bits (1172), Expect = 8.0e-127
Identity = 264/608 (43.42%), Postives = 355/608 (58.39%), Query Frame = 0

Query: 162 VWPESKSFSDEGYGPVPS---------------------RKLIGARFFNIGY----GELS 221
           VWPES+SFSD+GYG VPS                     RKLIGAR++N  +    G+L 
Sbjct: 153 VWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMKNTCNRKLIGARYYNKAFEAHNGQLD 212

Query: 222 VTFNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIKGGSPRARVASYKVCWP-EESG 281
              +++RD  GHGTHTLS AGGNFV GA V  +GNGT KGGSPRARVA+YKVCW   +  
Sbjct: 213 PLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRARVAAYKVCWSLTDPA 272

Query: 282 ECLDPNTLAAFEAAIGDGVDVISISVGGE----PKEFFSDALSVGAFHAVERGIVVVCSA 341
            C   + LAA + AI DGVDVI++S G       +  F+D +S+GAFHA+ + I++V SA
Sbjct: 273 SCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGAFHAISKNILLVASA 332

Query: 342 GNVGPTPGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKGTSFSSKALPFNKFYPLI 401
           GN GPTPGTV+NV+PW+ T+ AS++DRDF++ + + + + ++G S     LP N+ + LI
Sbjct: 333 GNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTI-NNQLIEGASLFVN-LPPNQAFSLI 392

Query: 402 NAVDAKANNVSNSDAEVCEEGSLDPKKLTGKIVVCLR-GGLPRVSKGYVAAKAGAVGMIL 461
            + DAK  N +  DA++C  G+LD  K+ GKIV+C R G +  V++G  A  AGA GMIL
Sbjct: 393 LSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTREGKIKSVAEGLEALTAGARGMIL 452

Query: 462 VNDEESGNAILTDLHIL------------------------------------------- 521
            N  ++G  +  + H+                                            
Sbjct: 453 NNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTTAIGDEDDPLKTGDTIKMSRARTL 512

Query: 522 ----PAS------------------HPDITAPGVNILAAYPDGVPLTELPLDDRQA-PFK 581
               PA                    PD+TAPGVNILAAY +    + L +D+R+   F 
Sbjct: 513 FGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDNRRGFKFN 572

Query: 582 VDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILD-FTGLIATP 641
           V  GTSM+CPH +GI GLLKT +P WSPAAIKSAIMTTA T DN   PI D F   +A  
Sbjct: 573 VLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADA 632

Query: 642 LAYGAGHVNPNSAMDPGLIYDITIDDYLNFLCARGYNATQIEKIS-KKIFVCDRSFKVTD 671
            AYG+GHV P+ A++PGL+YD+++ DYLNFLCA GY+   I  ++  + F+C  S  V D
Sbjct: 633 FAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICSGSHSVND 692

HSP 2 Score: 49.3 bits (116), Expect = 2.3e-04
Identity = 20/29 (68.97%), Postives = 28/29 (96.55%), Query Frame = 0

Query: 132 AKEAILYSYNKNINGFAAMLDEKQTADLA 161
           AKEAI+YSYN++INGFAA+L+E++ AD+A
Sbjct: 71  AKEAIIYSYNRHINGFAALLEEEEAADIA 99

BLAST of Lsi07G014450 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 1.7e-121
Identity = 263/615 (42.76%), Postives = 354/615 (57.56%), Query Frame = 0

Query: 162 VWPESKSFSDEGYGPVPS------------------RKLIGARFFNIGYG------ELSV 221
           VWPESKS+SDEG+GP+PS                  RKLIGARFF  GY       + S 
Sbjct: 142 VWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESK 201

Query: 222 TFNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIKGGSPRARVASYKVCWPEESGEC 281
              S RD++GHGTHT S A G+ V GA++LG  +GT +G +PRARVA YKVCW    G C
Sbjct: 202 ESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW---LGGC 261

Query: 282 LDPNTLAAFEAAIGDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVCSAGNVGPT 341
              + LAA + AI D V+V+S+S+GG   +++ D +++GAF A+ERGI+V CSAGN GP+
Sbjct: 262 FSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPS 321

Query: 342 PGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKGTS-FSSKALPFNKFYPLINAVDA 401
             ++SNV+PWI TVGA ++DRDF    +LG+ K   G S F  +ALP +K  P I A   
Sbjct: 322 SSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALP-DKLLPFIYA--G 381

Query: 402 KANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGLPRVSKGYVAAKAGAVGMILVNDEES 461
            A+N +N +  +C  G+L P+K+ GKIV+C RG   RV KG V   AG VGMIL N   +
Sbjct: 382 NASNATNGN--LCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAAN 441

Query: 462 GNAILTDLHILPAS---------------------------------------------- 521
           G  ++ D H+LPA+                                              
Sbjct: 442 GEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRG 501

Query: 522 ---------HPDITAPGVNILAAYPDGVPLTELPLDDRQAPFKVDSGTSMACPHVAGIVG 581
                     PD+ APGVNILAA+      T L  D R+  F + SGTSM+CPHV+G+  
Sbjct: 502 PNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAA 561

Query: 582 LLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILDF-TGLIATPLAYGAGHVNPNSAMDPG 641
           LLK+++P+WSPAAI+SA+MTTA  T  +  P+LD  TG  +TP  +GAGHV+P +A +PG
Sbjct: 562 LLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPG 621

Query: 642 LIYDITIDDYLNFLCARGYNATQIEKISKKIFVCD--RSFKVTDLNYPSISVTDLKMGPV 693
           LIYD+T +DYL FLCA  Y + QI  +S++ + CD  +S+ V DLNYPS +V    +G  
Sbjct: 622 LIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAY 681

BLAST of Lsi07G014450 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 4.3e-112
Identity = 243/599 (40.57%), Postives = 333/599 (55.59%), Query Frame = 0

Query: 162 VWPESKSFSDEGYGPVP------------------SRKLIGARFFNIGY----GELSVT- 221
           VWPE  SF D G GPVP                  +RKL+GARFF  GY    G+++ T 
Sbjct: 140 VWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETT 199

Query: 222 -FNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIKGGSPRARVASYKVCWPEESGEC 281
            F S RD++GHGTHT SI+ G +V  A+ LG  +G   G +P+AR+A+YKVCW   +  C
Sbjct: 200 EFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW---NSGC 259

Query: 282 LDPNTLAAFEAAIGDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVCSAGNVGPT 341
            D + LAAF+ A+ DGVDVIS+SVGG    ++ DA+++GAF A++RGI V  SAGN GP 
Sbjct: 260 YDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPG 319

Query: 342 PGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKGTS-FSSKALPFNKFYPLINAVDA 401
             TV+NV+PW+ TVGA +IDRDF   V LG+ K + G S +    L   + YPL+     
Sbjct: 320 ALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSL 379

Query: 402 KANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGLPRVSKGYVAAKAGAVGMILVNDEES 461
              +  +S   +C EGSLDP  + GKIV+C RG   R +KG +  K G +GMI+ N    
Sbjct: 380 LGGDGYSS--SLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFD 439

Query: 462 GNAILTDLHILPAS---------------------------------------------- 521
           G  ++ D H+LPA+                                              
Sbjct: 440 GEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVA 499

Query: 522 ---------------HPDITAPGVNILAAYPDGVPLTELPLDDRQAPFKVDSGTSMACPH 581
                           PD+ APG+NILAA+PD +  + +  D+R+  F + SGTSMACPH
Sbjct: 500 SFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPH 559

Query: 582 VAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILD-FTGLIATPLAYGAGHVNPN 641
           V+G+  LLK  +P WSPAAI+SA++TTA T DN+  P++D  TG  ++ + YG+GHV+P 
Sbjct: 560 VSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPT 619

Query: 642 SAMDPGLIYDITIDDYLNFLCARGYNATQIEKISKKIFVCD---RSFKVTDLNYPSISVT 666
            AMDPGL+YDIT  DY+NFLC   Y  T I  I+++   CD   R+  V +LNYPS SV 
Sbjct: 620 KAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVV 679

BLAST of Lsi07G014450 vs. ExPASy TrEMBL
Match: A0A5D3D737 (Subtilisin-like protease SBT5.3 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00080 PE=3 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 6.3e-260
Identity = 476/676 (70.41%), Postives = 507/676 (75.00%), Query Frame = 0

Query: 129 KKIAKEAILYSYNKNINGFAAMLDEKQTADLA---------------------------- 188
           KKIAKE ILYSYNKNINGF AMLDE+Q  DLA                            
Sbjct: 47  KKIAKEVILYSYNKNINGFVAMLDERQATDLAKFPHVVSVFESRSRKLHTTQSWKFLGVE 106

Query: 189 ----------------------------SVWPESKSFSDEGYGPVPS------------- 248
                                        VWPESKSFSDEGYGP+PS             
Sbjct: 107 KHEEIPTSNSIWNVTRFGEDIIIANFDTGVWPESKSFSDEGYGPIPSRWMGTCQSDADPK 166

Query: 249 ----RKLIGARFFNIGYGELSVTFNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIK 308
               RKLIGARFFNIGYGEL+ TFNSSRDN GHGTHTLSIAGGNFV GANVLG+GNGT+K
Sbjct: 167 FRCNRKLIGARFFNIGYGELADTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGLGNGTVK 226

Query: 309 GGSPRARVASYKVCWPEESGECLDPNTLAAFEAAIGDGVDVISISVGGEPKEFFSDALSV 368
           GGSPRARVASYKVCWP+E+ EC+DPNTLAAFEAAI DGVDVISISVGGEPKEFFSDALSV
Sbjct: 227 GGSPRARVASYKVCWPDEANECMDPNTLAAFEAAIDDGVDVISISVGGEPKEFFSDALSV 286

Query: 369 GAFHAVERGIVVVCSAGNVGPTPGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKGT 428
           GAFHAVERGIVVV SAGN+GPTPGTVSNVSPWILTVGAS+ DR FTNFV+LG+KKK KGT
Sbjct: 287 GAFHAVERGIVVVSSAGNMGPTPGTVSNVSPWILTVGASTTDRGFTNFVILGNKKKFKGT 346

Query: 429 SFSSKALPFNKFYPLINAVDAKANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGLPRVS 488
           SFSSK LP NKFYPLINAVDAKA NVS SDAEVC+EGSLDPKKL GKIVVCLRGGL RVS
Sbjct: 347 SFSSKVLPVNKFYPLINAVDAKAKNVSVSDAEVCDEGSLDPKKLAGKIVVCLRGGLSRVS 406

Query: 489 KGYVAAKAGAVGMILVNDEESGNAILTDLHILPASH------------------------ 548
           KGYVAAKAGAVGMI+ NDE+SGNAI+TD H+LPASH                        
Sbjct: 407 KGYVAAKAGAVGMIVENDEDSGNAIITDSHVLPASHVTYDDSISIFQYINSTKRPKAYIS 466

Query: 549 -------------------------------PDITAPGVNILAAYPDGVPLTELPLDDRQ 608
                                          PDITAPGVNILAAYPDG+PLTE PLDDRQ
Sbjct: 467 SVMTELEITPSAVVADFSSRGPNTIEESILKPDITAPGVNILAAYPDGIPLTEAPLDDRQ 526

Query: 609 APFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILDFTGLI 668
           +PFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNN +PI+D  GL 
Sbjct: 527 SPFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDHRGLE 586

Query: 669 ATPLAYGAGHVNPNSAMDPGLIYDITIDDYLNFLCARGYNATQIEKISKKIFVCDRSFKV 677
           A PLAYGAGHVNPNSAMDPGL+YDITIDDYLNFLCARGYN  QI++ISKK F+CD+SFKV
Sbjct: 587 ANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFLCARGYNTKQIKRISKKNFICDKSFKV 646

BLAST of Lsi07G014450 vs. ExPASy TrEMBL
Match: A0A1S3BL27 (subtilisin-like protease SBT5.3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491006 PE=3 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 1.4e-251
Identity = 450/587 (76.66%), Postives = 479/587 (81.60%), Query Frame = 0

Query: 162 VWPESKSFSDEGYGPVPS-----------------RKLIGARFFNIGYGELSVTFNSSRD 221
           VWPESKSFSDEGYGP+PS                 RKLIGARFFNIGYGEL+ TFNSSRD
Sbjct: 69  VWPESKSFSDEGYGPIPSRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELADTFNSSRD 128

Query: 222 NEGHGTHTLSIAGGNFVSGANVLGMGNGTIKGGSPRARVASYKVCWPEESGECLDPNTLA 281
           N GHGTHTLSIAGGNFV GANVLG+GNGT+KGGSPRARVASYKVCWP+E+ EC+DPNTLA
Sbjct: 129 NVGHGTHTLSIAGGNFVPGANVLGLGNGTVKGGSPRARVASYKVCWPDEANECMDPNTLA 188

Query: 282 AFEAAIGDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVCSAGNVGPTPGTVSNV 341
           AFEAAI DGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVV SAGNVGPTPGTVSNV
Sbjct: 189 AFEAAIDDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTVSNV 248

Query: 342 SPWILTVGASSIDRDFTNFVVLGSKKKLKGTSFSSKALPFNKFYPLINAVDAKANNVSNS 401
           SPWILTVGAS+ DR FTNFV+LG+KKK KGTSFSSK LP NKFYPLINAVDAKA NVS S
Sbjct: 249 SPWILTVGASTTDRGFTNFVILGNKKKFKGTSFSSKVLPVNKFYPLINAVDAKAKNVSVS 308

Query: 402 DAEVCEEGSLDPKKLTGKIVVCLRGGLPRVSKGYVAAKAGAVGMILVNDEESGNAILTDL 461
           DAEVC+EGSLDPKKL GKIVVCLRGGL RVSKGYVAAKAGAVGMI+ NDE+SGNAI+TD 
Sbjct: 309 DAEVCDEGSLDPKKLAGKIVVCLRGGLSRVSKGYVAAKAGAVGMIVENDEDSGNAIITDS 368

Query: 462 HILPASH----------------------------------------------------- 521
           H+LPASH                                                     
Sbjct: 369 HVLPASHVTYDDSISIFQYINSTKRPKAYISSVMTELEITPSAVVADFSSRGPNTIEESI 428

Query: 522 --PDITAPGVNILAAYPDGVPLTELPLDDRQAPFKVDSGTSMACPHVAGIVGLLKTLNPK 581
             PDITAPGVNILAAYPDG+PLTE PLDDRQ+PFKVDSGTSMACPHVAGIVGLLKTLNPK
Sbjct: 429 LKPDITAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHVAGIVGLLKTLNPK 488

Query: 582 WSPAAIKSAIMTTAKTTDNNLHPILDFTGLIATPLAYGAGHVNPNSAMDPGLIYDITIDD 641
           WSPAAIKSAIMTTAKTTDNN +PI+D  GL A PLAYGAGHVNPNSAMDPGL+YDITIDD
Sbjct: 489 WSPAAIKSAIMTTAKTTDNNFNPIVDHRGLEANPLAYGAGHVNPNSAMDPGLVYDITIDD 548

Query: 642 YLNFLCARGYNATQIEKISKKIFVCDRSFKVTDLNYPSISVTDLKMGPVTINRKVKNVGS 677
           YLNFLCARGYN  QI++ISKK F+CD+SFKVTDLNYPSISVT+LKMGPV INRK+KNVGS
Sbjct: 549 YLNFLCARGYNTKQIKRISKKNFICDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVGS 608

BLAST of Lsi07G014450 vs. ExPASy TrEMBL
Match: A0A1S3BLZ2 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491006 PE=3 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 3.3e-248
Identity = 450/616 (73.05%), Postives = 479/616 (77.76%), Query Frame = 0

Query: 162 VWPESKSFSDEGYGPVPS------------------------------------------ 221
           VWPESKSFSDEGYGP+PS                                          
Sbjct: 69  VWPESKSFSDEGYGPIPSRWMGTCQSDADPKFRCNRSRDSCPISVFFSSLSIISSKFKNG 128

Query: 222 ----RKLIGARFFNIGYGELSVTFNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIK 281
               RKLIGARFFNIGYGEL+ TFNSSRDN GHGTHTLSIAGGNFV GANVLG+GNGT+K
Sbjct: 129 YIKCRKLIGARFFNIGYGELADTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGLGNGTVK 188

Query: 282 GGSPRARVASYKVCWPEESGECLDPNTLAAFEAAIGDGVDVISISVGGEPKEFFSDALSV 341
           GGSPRARVASYKVCWP+E+ EC+DPNTLAAFEAAI DGVDVISISVGGEPKEFFSDALSV
Sbjct: 189 GGSPRARVASYKVCWPDEANECMDPNTLAAFEAAIDDGVDVISISVGGEPKEFFSDALSV 248

Query: 342 GAFHAVERGIVVVCSAGNVGPTPGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKGT 401
           GAFHAVERGIVVV SAGNVGPTPGTVSNVSPWILTVGAS+ DR FTNFV+LG+KKK KGT
Sbjct: 249 GAFHAVERGIVVVSSAGNVGPTPGTVSNVSPWILTVGASTTDRGFTNFVILGNKKKFKGT 308

Query: 402 SFSSKALPFNKFYPLINAVDAKANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGLPRVS 461
           SFSSK LP NKFYPLINAVDAKA NVS SDAEVC+EGSLDPKKL GKIVVCLRGGL RVS
Sbjct: 309 SFSSKVLPVNKFYPLINAVDAKAKNVSVSDAEVCDEGSLDPKKLAGKIVVCLRGGLSRVS 368

Query: 462 KGYVAAKAGAVGMILVNDEESGNAILTDLHILPASH------------------------ 521
           KGYVAAKAGAVGMI+ NDE+SGNAI+TD H+LPASH                        
Sbjct: 369 KGYVAAKAGAVGMIVENDEDSGNAIITDSHVLPASHVTYDDSISIFQYINSTKRPKAYIS 428

Query: 522 -------------------------------PDITAPGVNILAAYPDGVPLTELPLDDRQ 581
                                          PDITAPGVNILAAYPDG+PLTE PLDDRQ
Sbjct: 429 SVMTELEITPSAVVADFSSRGPNTIEESILKPDITAPGVNILAAYPDGIPLTEAPLDDRQ 488

Query: 582 APFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILDFTGLI 641
           +PFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNN +PI+D  GL 
Sbjct: 489 SPFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDHRGLE 548

Query: 642 ATPLAYGAGHVNPNSAMDPGLIYDITIDDYLNFLCARGYNATQIEKISKKIFVCDRSFKV 677
           A PLAYGAGHVNPNSAMDPGL+YDITIDDYLNFLCARGYN  QI++ISKK F+CD+SFKV
Sbjct: 549 ANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFLCARGYNTKQIKRISKKNFICDKSFKV 608

BLAST of Lsi07G014450 vs. ExPASy TrEMBL
Match: A0A0A0L617 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045160 PE=3 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 1.1e-243
Identity = 434/547 (79.34%), Postives = 461/547 (84.28%), Query Frame = 0

Query: 185 ARFFNIGYGELSVTFNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIKGGSPRARVA 244
           ARFFNIGYGEL+ TFNSSRDN GHGTHTLSIAGGNFV GANVLGMGNGT+KGGSPRARVA
Sbjct: 99  ARFFNIGYGELTDTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVA 158

Query: 245 SYKVCWPEESGECLDPNTLAAFEAAIGDGVDVISISVGGEPKEFFSDALSVGAFHAVERG 304
           SYKVCWP+E+ EC+DPNTLAAFEAAI DGVDVISISVGGEPKEFFSDALSVGAFHAVERG
Sbjct: 159 SYKVCWPDETNECVDPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERG 218

Query: 305 IVVVCSAGNVGPTPGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKGTSFSSKALPF 364
           IVVV SAGNVGPTPGTVSNVSPWILTVGAS+IDR FTNFVVLG+KKK KGTSFSSK LP 
Sbjct: 219 IVVVSSAGNVGPTPGTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKFKGTSFSSKVLPV 278

Query: 365 NKFYPLINAVDAKANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGLPRVSKGYVAAKAG 424
           NKFYPLINAVDAKANNVS SDAEVC+EGSLDP+KL GKIVVCLRGGLPRVSKGYVAAKAG
Sbjct: 279 NKFYPLINAVDAKANNVSVSDAEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAG 338

Query: 425 AVGMILVNDEESGNAILTDLHILPASH--------------------------------- 484
           AVGM++VNDEESGNAILTD H+LPASH                                 
Sbjct: 339 AVGMLVVNDEESGNAILTDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEIT 398

Query: 485 ----------------------PDITAPGVNILAAYPDGVPLTELPLDDRQAPFKVDSGT 544
                                 PDI APGVNILAAYPDG+PLTE PLDDRQ+PFKVDSGT
Sbjct: 399 PSPVVADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGT 458

Query: 545 SMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILDFTGLIATPLAYGAG 604
           SMACPH+AGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNN +PI+D+ GL A PLAYGAG
Sbjct: 459 SMACPHIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAG 518

Query: 605 HVNPNSAMDPGLIYDITIDDYLNFLCARGYNATQIEKISKKIFVCDRSFKVTDLNYPSIS 664
           HVNPNSAMDPGL+YDITIDDYLNFLCARGYN TQI++ISKK FVCD+SFKVTDLNYPSIS
Sbjct: 519 HVNPNSAMDPGLVYDITIDDYLNFLCARGYNTTQIKRISKKNFVCDKSFKVTDLNYPSIS 578

Query: 665 VTDLKMGPVTINRKVKNVGSPGIYVARVKAPLEVCIIVEPSTLHFTAMDEEKSFKVVLYS 677
           VT+LKMGPV INRK+KNVGSPG YVARVK PLEV IIVEP  L FTAMDEEKSFKV+L  
Sbjct: 579 VTNLKMGPVAINRKLKNVGSPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNR 638

BLAST of Lsi07G014450 vs. ExPASy TrEMBL
Match: A0A6J1KUM0 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498350 PE=3 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 2.0e-242
Identity = 446/675 (66.07%), Postives = 491/675 (72.74%), Query Frame = 0

Query: 129 KKIAKEAILYSYNKNINGFAAMLDEKQTADLA---------------------------- 188
           K IA+EAI +SYN++INGFAAMLDE Q ++LA                            
Sbjct: 66  KNIAEEAISHSYNRHINGFAAMLDENQVSELAKFPDVVSVFECQARTLHTTRSWNFLGME 125

Query: 189 ---------------------------SVWPESKSFSDEGYGPVPSR------------- 248
                                       VWPE+KSFSDEGYGP+PSR             
Sbjct: 126 KHEGIPSTSIWNIARFGGDTIIANFDSGVWPEAKSFSDEGYGPIPSRWKGTCQSDSDPNF 185

Query: 249 ----KLIGARFFNIGYGELSVTFNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIKG 308
               KLIGARFFN GYG L+ TFNS RD EGHGTHTLSIAGGNFVSGANV  M NGT KG
Sbjct: 186 HCNKKLIGARFFNKGYGNLNATFNSPRDQEGHGTHTLSIAGGNFVSGANVFDMANGTAKG 245

Query: 309 GSPRARVASYKVCWPEESGECLDPNTLAAFEAAIGDGVDVISISVGGEPKEFFSDALSVG 368
           GSPRAR+ASYKVCWP E  +CLDPN LAA++AAI DGVDVIS+S+GGEPKEF  DALSVG
Sbjct: 246 GSPRARIASYKVCWPVEGRQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVG 305

Query: 369 AFHAVERGIVVVCSAGNVGPTPGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKGTS 428
           AFHAV+ GIVVVCSAGN GPTP TVSNVSPW+LTVGAS+IDRDFTNFVVLG+KKKLKGTS
Sbjct: 306 AFHAVQHGIVVVCSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTS 365

Query: 429 FSSKALPFNKFYPLINAVDAKANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGLPRVSK 488
           FSSKAL FNKFYPLINAVDAKANN S+SDAEVC E SLDP KL GKIVVCLRG + RVSK
Sbjct: 366 FSSKALAFNKFYPLINAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSK 425

Query: 489 GYVAAKAGAVGMILVNDEESGNAILTDLHILPASH------------------------- 548
           GYV A+AGA GMILVND+++G+AI TDLH+LPASH                         
Sbjct: 426 GYVVAQAGAAGMILVNDKDNGDAIATDLHLLPASHVTFNDGISIFHYIKSTKTPMACISS 485

Query: 549 ------------------------------PDITAPGVNILAAYPDGVPLTELPLDDRQA 608
                                         PDITAPGVNI+AAYPD +PL EL +DDR+A
Sbjct: 486 VKTELDVNPSPVMADFSSRGPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRA 545

Query: 609 PFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILDFTGLIA 668
           PFKVDSGTSMACPHVAGIVGLLK+  PKWSPAAIKSAIMTTAKT+ NN +PILDFTGL A
Sbjct: 546 PFKVDSGTSMACPHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKTSANNFNPILDFTGLEA 605

Query: 669 TPLAYGAGHVNPNSAMDPGLIYDITIDDYLNFLCARGYNATQIEKISKKIFVCDRSFKVT 677
           TPLAYG GHV+PNS MDPGL+YDI IDDYLNFLCARG NATQI K+S K+FVCD SFKVT
Sbjct: 606 TPLAYGNGHVDPNSVMDPGLVYDIAIDDYLNFLCARGDNATQINKLSHKLFVCDPSFKVT 665

BLAST of Lsi07G014450 vs. NCBI nr
Match: XP_038903594.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 958.7 bits (2477), Expect = 2.9e-275
Identity = 509/680 (74.85%), Postives = 530/680 (77.94%), Query Frame = 0

Query: 129 KKIAKEAILYSYNKNINGFAAMLDEKQTADLA---------------------------- 188
           K IAKEAILYSYNK+INGFAAMLDEKQ ADLA                            
Sbjct: 66  KMIAKEAILYSYNKHINGFAAMLDEKQAADLAKFPNVVSVFESQARKLHTTRSWKFLGIE 125

Query: 189 ---------------------------SVWPESKSFSDEGYGPVPS-------------- 248
                                       VWPESKSFSDEGYGP+PS              
Sbjct: 126 KHEEIPSNYIWNVARFGDDIIIANFDTGVWPESKSFSDEGYGPIPSRWKGTCQSDPKFHC 185

Query: 249 -RKLIGARFFNIGYGELSVTFNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIKGGS 308
            RKLIGARFFN+GYGELSVTFNSS+DN GHGTHTLSIAGGNFVSGANVLGMGNGTIKGGS
Sbjct: 186 NRKLIGARFFNLGYGELSVTFNSSKDNVGHGTHTLSIAGGNFVSGANVLGMGNGTIKGGS 245

Query: 309 PRARVASYKVCWPEESGECLDPNTLAAFEAAIGDGVDVISISVGGEPKEFFSDALSVGAF 368
           PRARVASYKVCWP E+ ECLDPNTLAAFEAAI DGVDVISISVG EPKEFFSDALSVGAF
Sbjct: 246 PRARVASYKVCWPAEASECLDPNTLAAFEAAIDDGVDVISISVGAEPKEFFSDALSVGAF 305

Query: 369 HAVERGIVVVCSAGNVGPTPGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKGTSFS 428
           HAVERGIVVVCSAGNVGPTPGTVSNVSPWILTVGAS+IDRDFTNFVVLG+KKK KGTSFS
Sbjct: 306 HAVERGIVVVCSAGNVGPTPGTVSNVSPWILTVGASTIDRDFTNFVVLGNKKKFKGTSFS 365

Query: 429 SKALPFNKFYPLINAVDAKANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGLPRVSKGY 488
           SKALPFNK YPLINAVDAKANNVSNSDAEVCEEGSLDP+KLTGKIVVCLRGGLPRVSKGY
Sbjct: 366 SKALPFNKLYPLINAVDAKANNVSNSDAEVCEEGSLDPEKLTGKIVVCLRGGLPRVSKGY 425

Query: 489 VAAKAGAVGMILVNDEESGNAILTDLHILPASH--------------------------- 548
           VAAKAGA GMILVNDEESGNAILTDLHILPASH                           
Sbjct: 426 VAAKAGAAGMILVNDEESGNAILTDLHILPASHITYNDSISIFQYINSTKTPMAYISSVM 485

Query: 549 ----------------------------PDITAPGVNILAAYPDGVPLTELPLDDRQAPF 608
                                       PDITAPGVNI+AAYP+GVPLT+LPLDDRQAPF
Sbjct: 486 TELEIKPSPVMADFSSRGPNTIEESILKPDITAPGVNIMAAYPNGVPLTKLPLDDRQAPF 545

Query: 609 KVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILDFTGLIATP 668
            VDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKT DN+LHPILD TGL ATP
Sbjct: 546 NVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNSLHPILDSTGLKATP 605

Query: 669 LAYGAGHVNPNSAMDPGLIYDITIDDYLNFLCARGYNATQIEKISKKIFVCDRSFKVTDL 684
           LAYGAGHVNPNSAMDPGL+YDITIDDYLNFLCARGYNATQI++ISKKIFVCDRSFKVTDL
Sbjct: 606 LAYGAGHVNPNSAMDPGLVYDITIDDYLNFLCARGYNATQIKRISKKIFVCDRSFKVTDL 665

BLAST of Lsi07G014450 vs. NCBI nr
Match: KAE8650099.1 (hypothetical protein Csa_011439 [Cucumis sativus])

HSP 1 Score: 941.8 bits (2433), Expect = 3.7e-270
Identity = 481/620 (77.58%), Postives = 511/620 (82.42%), Query Frame = 0

Query: 129 KKIAKEAILYSYNKNINGFAAMLDEKQTADLASVWPESKSFSDEGYGPVP---------- 188
           KK A+E ILYSYNKNINGF AMLDEKQ  DL SVWPESKSFSDEGYGP+P          
Sbjct: 74  KKTAEEVILYSYNKNINGFVAMLDEKQATDLTSVWPESKSFSDEGYGPIPPRWMGTCQSD 133

Query: 189 -------SRKLIGARFFNIGYGELSVTFNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGN 248
                  +RKLIGARFFNIGYGEL+ TFNSSRDN GHGTHTLSIAGGNFV GANVLGMGN
Sbjct: 134 ADPKFRCNRKLIGARFFNIGYGELTDTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGN 193

Query: 249 GTIKGGSPRARVASYKVCWPEESGECLDPNTLAAFEAAIGDGVDVISISVGGEPKEFFSD 308
           GT+KGGSPRARVASYKVCWP+E+ EC+DPNTLAAFEAAI DGVDVISISVGGEPKEFFSD
Sbjct: 194 GTVKGGSPRARVASYKVCWPDETNECVDPNTLAAFEAAIEDGVDVISISVGGEPKEFFSD 253

Query: 309 ALSVGAFHAVERGIVVVCSAGNVGPTPGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKK 368
           ALSVGAFHAVERGIVVV SAGNVGPTPGTVSNVSPWILTVGAS+IDR FTNFVVLG+KKK
Sbjct: 254 ALSVGAFHAVERGIVVVSSAGNVGPTPGTVSNVSPWILTVGASTIDRGFTNFVVLGNKKK 313

Query: 369 LKGTSFSSKALPFNKFYPLINAVDAKANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGL 428
            KGTSFSSK LP NKFYPLINAVDAKANNVS SDAEVC+EGSLDP+KL GKIVVCLRGGL
Sbjct: 314 FKGTSFSSKVLPVNKFYPLINAVDAKANNVSVSDAEVCDEGSLDPEKLAGKIVVCLRGGL 373

Query: 429 PRVSKGYVAAKAGAVGMILVNDEESGNAILTDLHILPASH-------------------- 488
           PRVSKGYVAAKAGAVGM++VNDEESGNAILTD H+LPASH                    
Sbjct: 374 PRVSKGYVAAKAGAVGMLVVNDEESGNAILTDSHVLPASHVTYDDSISIFQYINSTKTPM 433

Query: 489 -----------------------------------PDITAPGVNILAAYPDGVPLTELPL 548
                                              PDI APGVNILAAYPDG+PLTE PL
Sbjct: 434 AYISSVMTELEITPSPVVADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLTEAPL 493

Query: 549 DDRQAPFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILDF 608
           DDRQ+PFKVDSGTSMACPH+AGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNN +PI+D+
Sbjct: 494 DDRQSPFKVDSGTSMACPHIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDY 553

Query: 609 TGLIATPLAYGAGHVNPNSAMDPGLIYDITIDDYLNFLCARGYNATQIEKISKKIFVCDR 668
            GL A PLAYGAGHVNPNSAMDPGL+YDITIDDYLNFLCARGYN TQI++ISKK FVCD+
Sbjct: 554 GGLEANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFLCARGYNTTQIKRISKKNFVCDK 613

Query: 669 SFKVTDLNYPSISVTDLKMGPVTINRKVKNVGSPGIYVARVKAPLEVCIIVEPSTLHFTA 677
           SFKVTDLNYPSISVT+LKMGPV INRK+KNVGSPG YVARVK PLEV IIVEP  L FTA
Sbjct: 614 SFKVTDLNYPSISVTNLKMGPVAINRKLKNVGSPGTYVARVKTPLEVSIIVEPRILDFTA 673

BLAST of Lsi07G014450 vs. NCBI nr
Match: TYK19379.1 (subtilisin-like protease SBT5.3 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 906.7 bits (2342), Expect = 1.3e-259
Identity = 476/676 (70.41%), Postives = 507/676 (75.00%), Query Frame = 0

Query: 129 KKIAKEAILYSYNKNINGFAAMLDEKQTADLA---------------------------- 188
           KKIAKE ILYSYNKNINGF AMLDE+Q  DLA                            
Sbjct: 47  KKIAKEVILYSYNKNINGFVAMLDERQATDLAKFPHVVSVFESRSRKLHTTQSWKFLGVE 106

Query: 189 ----------------------------SVWPESKSFSDEGYGPVPS------------- 248
                                        VWPESKSFSDEGYGP+PS             
Sbjct: 107 KHEEIPTSNSIWNVTRFGEDIIIANFDTGVWPESKSFSDEGYGPIPSRWMGTCQSDADPK 166

Query: 249 ----RKLIGARFFNIGYGELSVTFNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIK 308
               RKLIGARFFNIGYGEL+ TFNSSRDN GHGTHTLSIAGGNFV GANVLG+GNGT+K
Sbjct: 167 FRCNRKLIGARFFNIGYGELADTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGLGNGTVK 226

Query: 309 GGSPRARVASYKVCWPEESGECLDPNTLAAFEAAIGDGVDVISISVGGEPKEFFSDALSV 368
           GGSPRARVASYKVCWP+E+ EC+DPNTLAAFEAAI DGVDVISISVGGEPKEFFSDALSV
Sbjct: 227 GGSPRARVASYKVCWPDEANECMDPNTLAAFEAAIDDGVDVISISVGGEPKEFFSDALSV 286

Query: 369 GAFHAVERGIVVVCSAGNVGPTPGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKGT 428
           GAFHAVERGIVVV SAGN+GPTPGTVSNVSPWILTVGAS+ DR FTNFV+LG+KKK KGT
Sbjct: 287 GAFHAVERGIVVVSSAGNMGPTPGTVSNVSPWILTVGASTTDRGFTNFVILGNKKKFKGT 346

Query: 429 SFSSKALPFNKFYPLINAVDAKANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGLPRVS 488
           SFSSK LP NKFYPLINAVDAKA NVS SDAEVC+EGSLDPKKL GKIVVCLRGGL RVS
Sbjct: 347 SFSSKVLPVNKFYPLINAVDAKAKNVSVSDAEVCDEGSLDPKKLAGKIVVCLRGGLSRVS 406

Query: 489 KGYVAAKAGAVGMILVNDEESGNAILTDLHILPASH------------------------ 548
           KGYVAAKAGAVGMI+ NDE+SGNAI+TD H+LPASH                        
Sbjct: 407 KGYVAAKAGAVGMIVENDEDSGNAIITDSHVLPASHVTYDDSISIFQYINSTKRPKAYIS 466

Query: 549 -------------------------------PDITAPGVNILAAYPDGVPLTELPLDDRQ 608
                                          PDITAPGVNILAAYPDG+PLTE PLDDRQ
Sbjct: 467 SVMTELEITPSAVVADFSSRGPNTIEESILKPDITAPGVNILAAYPDGIPLTEAPLDDRQ 526

Query: 609 APFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILDFTGLI 668
           +PFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNN +PI+D  GL 
Sbjct: 527 SPFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDHRGLE 586

Query: 669 ATPLAYGAGHVNPNSAMDPGLIYDITIDDYLNFLCARGYNATQIEKISKKIFVCDRSFKV 677
           A PLAYGAGHVNPNSAMDPGL+YDITIDDYLNFLCARGYN  QI++ISKK F+CD+SFKV
Sbjct: 587 ANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFLCARGYNTKQIKRISKKNFICDKSFKV 646

BLAST of Lsi07G014450 vs. NCBI nr
Match: XP_008449005.1 (PREDICTED: subtilisin-like protease SBT5.3 isoform X2 [Cucumis melo])

HSP 1 Score: 879.0 bits (2270), Expect = 2.9e-251
Identity = 450/587 (76.66%), Postives = 479/587 (81.60%), Query Frame = 0

Query: 162 VWPESKSFSDEGYGPVPS-----------------RKLIGARFFNIGYGELSVTFNSSRD 221
           VWPESKSFSDEGYGP+PS                 RKLIGARFFNIGYGEL+ TFNSSRD
Sbjct: 69  VWPESKSFSDEGYGPIPSRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELADTFNSSRD 128

Query: 222 NEGHGTHTLSIAGGNFVSGANVLGMGNGTIKGGSPRARVASYKVCWPEESGECLDPNTLA 281
           N GHGTHTLSIAGGNFV GANVLG+GNGT+KGGSPRARVASYKVCWP+E+ EC+DPNTLA
Sbjct: 129 NVGHGTHTLSIAGGNFVPGANVLGLGNGTVKGGSPRARVASYKVCWPDEANECMDPNTLA 188

Query: 282 AFEAAIGDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVCSAGNVGPTPGTVSNV 341
           AFEAAI DGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVV SAGNVGPTPGTVSNV
Sbjct: 189 AFEAAIDDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTVSNV 248

Query: 342 SPWILTVGASSIDRDFTNFVVLGSKKKLKGTSFSSKALPFNKFYPLINAVDAKANNVSNS 401
           SPWILTVGAS+ DR FTNFV+LG+KKK KGTSFSSK LP NKFYPLINAVDAKA NVS S
Sbjct: 249 SPWILTVGASTTDRGFTNFVILGNKKKFKGTSFSSKVLPVNKFYPLINAVDAKAKNVSVS 308

Query: 402 DAEVCEEGSLDPKKLTGKIVVCLRGGLPRVSKGYVAAKAGAVGMILVNDEESGNAILTDL 461
           DAEVC+EGSLDPKKL GKIVVCLRGGL RVSKGYVAAKAGAVGMI+ NDE+SGNAI+TD 
Sbjct: 309 DAEVCDEGSLDPKKLAGKIVVCLRGGLSRVSKGYVAAKAGAVGMIVENDEDSGNAIITDS 368

Query: 462 HILPASH----------------------------------------------------- 521
           H+LPASH                                                     
Sbjct: 369 HVLPASHVTYDDSISIFQYINSTKRPKAYISSVMTELEITPSAVVADFSSRGPNTIEESI 428

Query: 522 --PDITAPGVNILAAYPDGVPLTELPLDDRQAPFKVDSGTSMACPHVAGIVGLLKTLNPK 581
             PDITAPGVNILAAYPDG+PLTE PLDDRQ+PFKVDSGTSMACPHVAGIVGLLKTLNPK
Sbjct: 429 LKPDITAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHVAGIVGLLKTLNPK 488

Query: 582 WSPAAIKSAIMTTAKTTDNNLHPILDFTGLIATPLAYGAGHVNPNSAMDPGLIYDITIDD 641
           WSPAAIKSAIMTTAKTTDNN +PI+D  GL A PLAYGAGHVNPNSAMDPGL+YDITIDD
Sbjct: 489 WSPAAIKSAIMTTAKTTDNNFNPIVDHRGLEANPLAYGAGHVNPNSAMDPGLVYDITIDD 548

Query: 642 YLNFLCARGYNATQIEKISKKIFVCDRSFKVTDLNYPSISVTDLKMGPVTINRKVKNVGS 677
           YLNFLCARGYN  QI++ISKK F+CD+SFKVTDLNYPSISVT+LKMGPV INRK+KNVGS
Sbjct: 549 YLNFLCARGYNTKQIKRISKKNFICDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVGS 608

BLAST of Lsi07G014450 vs. NCBI nr
Match: XP_008449002.1 (PREDICTED: subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo])

HSP 1 Score: 867.8 bits (2241), Expect = 6.7e-248
Identity = 450/616 (73.05%), Postives = 479/616 (77.76%), Query Frame = 0

Query: 162 VWPESKSFSDEGYGPVPS------------------------------------------ 221
           VWPESKSFSDEGYGP+PS                                          
Sbjct: 69  VWPESKSFSDEGYGPIPSRWMGTCQSDADPKFRCNRSRDSCPISVFFSSLSIISSKFKNG 128

Query: 222 ----RKLIGARFFNIGYGELSVTFNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIK 281
               RKLIGARFFNIGYGEL+ TFNSSRDN GHGTHTLSIAGGNFV GANVLG+GNGT+K
Sbjct: 129 YIKCRKLIGARFFNIGYGELADTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGLGNGTVK 188

Query: 282 GGSPRARVASYKVCWPEESGECLDPNTLAAFEAAIGDGVDVISISVGGEPKEFFSDALSV 341
           GGSPRARVASYKVCWP+E+ EC+DPNTLAAFEAAI DGVDVISISVGGEPKEFFSDALSV
Sbjct: 189 GGSPRARVASYKVCWPDEANECMDPNTLAAFEAAIDDGVDVISISVGGEPKEFFSDALSV 248

Query: 342 GAFHAVERGIVVVCSAGNVGPTPGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKGT 401
           GAFHAVERGIVVV SAGNVGPTPGTVSNVSPWILTVGAS+ DR FTNFV+LG+KKK KGT
Sbjct: 249 GAFHAVERGIVVVSSAGNVGPTPGTVSNVSPWILTVGASTTDRGFTNFVILGNKKKFKGT 308

Query: 402 SFSSKALPFNKFYPLINAVDAKANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGLPRVS 461
           SFSSK LP NKFYPLINAVDAKA NVS SDAEVC+EGSLDPKKL GKIVVCLRGGL RVS
Sbjct: 309 SFSSKVLPVNKFYPLINAVDAKAKNVSVSDAEVCDEGSLDPKKLAGKIVVCLRGGLSRVS 368

Query: 462 KGYVAAKAGAVGMILVNDEESGNAILTDLHILPASH------------------------ 521
           KGYVAAKAGAVGMI+ NDE+SGNAI+TD H+LPASH                        
Sbjct: 369 KGYVAAKAGAVGMIVENDEDSGNAIITDSHVLPASHVTYDDSISIFQYINSTKRPKAYIS 428

Query: 522 -------------------------------PDITAPGVNILAAYPDGVPLTELPLDDRQ 581
                                          PDITAPGVNILAAYPDG+PLTE PLDDRQ
Sbjct: 429 SVMTELEITPSAVVADFSSRGPNTIEESILKPDITAPGVNILAAYPDGIPLTEAPLDDRQ 488

Query: 582 APFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILDFTGLI 641
           +PFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNN +PI+D  GL 
Sbjct: 489 SPFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDHRGLE 548

Query: 642 ATPLAYGAGHVNPNSAMDPGLIYDITIDDYLNFLCARGYNATQIEKISKKIFVCDRSFKV 677
           A PLAYGAGHVNPNSAMDPGL+YDITIDDYLNFLCARGYN  QI++ISKK F+CD+SFKV
Sbjct: 549 ANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFLCARGYNTKQIKRISKKNFICDKSFKV 608

BLAST of Lsi07G014450 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 563.1 bits (1450), Expect = 3.3e-160
Identity = 313/679 (46.10%), Postives = 404/679 (59.50%), Query Frame = 0

Query: 119 RPFFAAPPPRKKIAKEAILYSYNKNINGFAAMLDEKQTADLA------------------ 178
           R F A+     + AKEAI YSY ++INGFAA+LDE + A++A                  
Sbjct: 68  RTFLASFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHT 127

Query: 179 -------------------------------------SVWPESKSFSDEGYGPVP----- 238
                                                 VWPESKSFSDEGYG VP     
Sbjct: 128 THSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKG 187

Query: 239 --------SRKLIGARFFNIGYGEL-----SVTFNSSRDNEGHGTHTLSIAGGNFVSGAN 298
                   +RKLIGAR+FN GY        + ++ + RD++GHG+HTLS A GNFV GAN
Sbjct: 188 RCHKDVPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGAN 247

Query: 299 VLGMGNGTIKGGSPRARVASYKVCWPEESG-ECLDPNTLAAFEAAIGDGVDVISISVGGE 358
           V G+GNGT  GGSP+ARVA+YKVCWP   G EC D + LAA EAAI DGVDV+S SVGG+
Sbjct: 248 VFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGD 307

Query: 359 PKEFFSDALSVGAFHAVERGIVVVCSAGNVGPTPGTVSNVSPWILTVGASSIDRDFTNFV 418
             ++ SD +++G+FHAV+ G+ VVCSAGN GP  GTVSNV+PW++TVGASS+DR+F  FV
Sbjct: 308 AGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFV 367

Query: 419 VLGSKKKLKGTSFSSKALPFNKFYPLINAVDAKANNVSNSDAEVCEEGSLDPKKLTGKIV 478
            L + +  KGTS  SK LP  K Y LI+A DA   N + +DA +C++GSLDPKK+ GKI+
Sbjct: 368 ELKNGQSFKGTSL-SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKIL 427

Query: 479 VCLRGGLPRVSKGYVAAKAGAVGMILVNDEESGNAILTDLHILPASH------------- 538
           VCLRG   RV KG  AA AGA GM+L ND+ SGN I++D H+LPAS              
Sbjct: 428 VCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYL 487

Query: 539 ------------------------------------------PDITAPGVNILAAYPDGV 598
                                                     PDITAPGVNI+AA+ +  
Sbjct: 488 SSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEAT 547

Query: 599 PLTELPLDDRQAPFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNN 658
             T+L  D+R+ PF  +SGTSM+CPH++G+VGLLKTL+P WSPAAI+SAIMTT++T +N 
Sbjct: 548 GPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNR 607

Query: 659 LHPILDFTGLIATPLAYGAGHVNPNSAMDPGLIYDITIDDYLNFLCARGYNATQIEKISK 668
             P++D +   A P +YG+GHV PN A  PGL+YD+T  DYL+FLCA GYN T ++  ++
Sbjct: 608 RKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAE 667

BLAST of Lsi07G014450 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 553.1 bits (1424), Expect = 3.4e-157
Identity = 310/676 (45.86%), Postives = 396/676 (58.58%), Query Frame = 0

Query: 132 AKEAILYSYNKNINGFAAMLDEKQTADLA------------------------------- 191
           A +AI YSY K+INGFAA LD     +++                               
Sbjct: 71  ATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNS 130

Query: 192 ------------------------SVWPESKSFSDEGYGPVPS----------------- 251
                                    VWPESKSF DEG GP+PS                 
Sbjct: 131 YVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCN 190

Query: 252 RKLIGARFFNIGY----GELSVTFNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIK 311
           RKLIGAR+FN GY    G L+ +F+S RD +GHG+HTLS A G+FV G ++ G GNGT K
Sbjct: 191 RKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAK 250

Query: 312 GGSPRARVASYKVCWPEESG-ECLDPNTLAAFEAAIGDGVDVISISVGGEPKEFFSDALS 371
           GGSPRARVA+YKVCWP   G EC D + LAAF+AAI DG DVIS+S+GGEP  FF+D+++
Sbjct: 251 GGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVA 310

Query: 372 VGAFHAVERGIVVVCSAGNVGPTPGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKG 431
           +G+FHA ++ IVVVCSAGN GP   TVSNV+PW +TVGAS++DR+F + +VLG+ K  KG
Sbjct: 311 IGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKG 370

Query: 432 TSFSSKALPFNKFYPLINAVDAKANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGLPRV 491
            S SS ALP  KFYP++ +V+AKA N S  DA++C+ GSLDP K  GKI+VCLRG   RV
Sbjct: 371 QSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRV 430

Query: 492 SKGYVAAKAGAVGMILVNDEESGNAILTDLHILPASH----------------------- 551
            KG   A  G +GM+L N   +GN +L D H+LPA+                        
Sbjct: 431 EKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHI 490

Query: 552 --------------------------------PDITAPGVNILAAYPDGVPLTELPLDDR 611
                                           PDITAPGV+++AAY   V  T    D R
Sbjct: 491 TPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPR 550

Query: 612 QAPFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILDFTGL 671
           +  F   SGTSM+CPH++GI GLLKT  P WSPAAI+SAIMTTA   D+   PI + T +
Sbjct: 551 RLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNM 610

Query: 672 IATPLAYGAGHVNPNSAMDPGLIYDITIDDYLNFLCARGYNATQIEKISKKIFVCDR-SF 675
            ATP ++GAGHV PN A++PGL+YD+ I DYLNFLC+ GYNA+QI   S   F C     
Sbjct: 611 KATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKI 670

BLAST of Lsi07G014450 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 438.3 bits (1126), Expect = 1.2e-122
Identity = 263/615 (42.76%), Postives = 354/615 (57.56%), Query Frame = 0

Query: 162 VWPESKSFSDEGYGPVPS------------------RKLIGARFFNIGYG------ELSV 221
           VWPESKS+SDEG+GP+PS                  RKLIGARFF  GY       + S 
Sbjct: 142 VWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESK 201

Query: 222 TFNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIKGGSPRARVASYKVCWPEESGEC 281
              S RD++GHGTHT S A G+ V GA++LG  +GT +G +PRARVA YKVCW    G C
Sbjct: 202 ESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW---LGGC 261

Query: 282 LDPNTLAAFEAAIGDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVCSAGNVGPT 341
              + LAA + AI D V+V+S+S+GG   +++ D +++GAF A+ERGI+V CSAGN GP+
Sbjct: 262 FSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPS 321

Query: 342 PGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKGTS-FSSKALPFNKFYPLINAVDA 401
             ++SNV+PWI TVGA ++DRDF    +LG+ K   G S F  +ALP +K  P I A   
Sbjct: 322 SSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALP-DKLLPFIYA--G 381

Query: 402 KANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGLPRVSKGYVAAKAGAVGMILVNDEES 461
            A+N +N +  +C  G+L P+K+ GKIV+C RG   RV KG V   AG VGMIL N   +
Sbjct: 382 NASNATNGN--LCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAAN 441

Query: 462 GNAILTDLHILPAS---------------------------------------------- 521
           G  ++ D H+LPA+                                              
Sbjct: 442 GEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRG 501

Query: 522 ---------HPDITAPGVNILAAYPDGVPLTELPLDDRQAPFKVDSGTSMACPHVAGIVG 581
                     PD+ APGVNILAA+      T L  D R+  F + SGTSM+CPHV+G+  
Sbjct: 502 PNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAA 561

Query: 582 LLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILDF-TGLIATPLAYGAGHVNPNSAMDPG 641
           LLK+++P+WSPAAI+SA+MTTA  T  +  P+LD  TG  +TP  +GAGHV+P +A +PG
Sbjct: 562 LLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPG 621

Query: 642 LIYDITIDDYLNFLCARGYNATQIEKISKKIFVCD--RSFKVTDLNYPSISVTDLKMGPV 693
           LIYD+T +DYL FLCA  Y + QI  +S++ + CD  +S+ V DLNYPS +V    +G  
Sbjct: 622 LIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAY 681

BLAST of Lsi07G014450 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 407.1 bits (1045), Expect = 3.0e-113
Identity = 243/599 (40.57%), Postives = 333/599 (55.59%), Query Frame = 0

Query: 162 VWPESKSFSDEGYGPVP------------------SRKLIGARFFNIGY----GELSVT- 221
           VWPE  SF D G GPVP                  +RKL+GARFF  GY    G+++ T 
Sbjct: 140 VWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETT 199

Query: 222 -FNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIKGGSPRARVASYKVCWPEESGEC 281
            F S RD++GHGTHT SI+ G +V  A+ LG  +G   G +P+AR+A+YKVCW   +  C
Sbjct: 200 EFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW---NSGC 259

Query: 282 LDPNTLAAFEAAIGDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVCSAGNVGPT 341
            D + LAAF+ A+ DGVDVIS+SVGG    ++ DA+++GAF A++RGI V  SAGN GP 
Sbjct: 260 YDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPG 319

Query: 342 PGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKGTS-FSSKALPFNKFYPLINAVDA 401
             TV+NV+PW+ TVGA +IDRDF   V LG+ K + G S +    L   + YPL+     
Sbjct: 320 ALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSL 379

Query: 402 KANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGLPRVSKGYVAAKAGAVGMILVNDEES 461
              +  +S   +C EGSLDP  + GKIV+C RG   R +KG +  K G +GMI+ N    
Sbjct: 380 LGGDGYSS--SLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFD 439

Query: 462 GNAILTDLHILPAS---------------------------------------------- 521
           G  ++ D H+LPA+                                              
Sbjct: 440 GEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVA 499

Query: 522 ---------------HPDITAPGVNILAAYPDGVPLTELPLDDRQAPFKVDSGTSMACPH 581
                           PD+ APG+NILAA+PD +  + +  D+R+  F + SGTSMACPH
Sbjct: 500 SFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPH 559

Query: 582 VAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILD-FTGLIATPLAYGAGHVNPN 641
           V+G+  LLK  +P WSPAAI+SA++TTA T DN+  P++D  TG  ++ + YG+GHV+P 
Sbjct: 560 VSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPT 619

Query: 642 SAMDPGLIYDITIDDYLNFLCARGYNATQIEKISKKIFVCD---RSFKVTDLNYPSISVT 666
            AMDPGL+YDIT  DY+NFLC   Y  T I  I+++   CD   R+  V +LNYPS SV 
Sbjct: 620 KAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVV 679

BLAST of Lsi07G014450 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 398.3 bits (1022), Expect = 1.4e-110
Identity = 243/598 (40.64%), Postives = 337/598 (56.35%), Query Frame = 0

Query: 162 VWPESKSFSDEGYGPVP------------------SRKLIGARFFN-------IGYGELS 221
           +WPE +SFSD   GP+P                  +RK+IGARFF        IG    +
Sbjct: 134 IWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIGGINKT 193

Query: 222 VTFNSSRDNEGHGTHTLSIAGGNFVSGANVLGMGNGTIKGGSPRARVASYKVCWPEESGE 281
           V F S RD +GHGTHT S A G     A++ G  +G  KG +P+AR+A+YKVCW ++SG 
Sbjct: 194 VEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCW-KDSG- 253

Query: 282 CLDPNTLAAFEAAIGDGVDVISISVGGE---PKEFFSDALSVGAFHAVERGIVVVCSAGN 341
           CLD + LAAF+AA+ DGVDVISIS+GG       ++ D +++G++ A  +GI V  SAGN
Sbjct: 254 CLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGN 313

Query: 342 VGPTPGTVSNVSPWILTVGASSIDRDFTNFVVLGSKKKLKGTSFSSKALPFN-KFYPLIN 401
            GP   +V+N++PW+ TVGAS+IDR+F    +LG   +L+G S  +  +P N + +P++ 
Sbjct: 314 EGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYA-GVPLNGRMFPVV- 373

Query: 402 AVDAKANNVSNSDAEVCEEGSLDPKKLTGKIVVCLRGGLPRVSKGYVAAKAGAVGMILVN 461
                      S A +C E +LDPK++ GKIV+C RG  PRV+KG V  KAG VGMIL N
Sbjct: 374 ----YPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILAN 433

Query: 462 DEESGNAILTDLHILPA----------------SH------------------------- 521
              +G  ++ D H++PA                SH                         
Sbjct: 434 GASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASF 493

Query: 522 --------------PDITAPGVNILAAYPDGVPLTELPLDDRQAPFKVDSGTSMACPHVA 581
                         PD+ APGVNILAA+ D V  T LP D R+  F + SGTSMACPHV+
Sbjct: 494 SGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVS 553

Query: 582 GIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNLHPILD-FTGLIATPLAYGAGHVNPNSA 641
           G   LLK+ +P WSPA I+SA+MTT    DN+   ++D  TG  ATP  YG+GH+N   A
Sbjct: 554 GAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRA 613

Query: 642 MDPGLIYDITIDDYLNFLCARGYNATQIEKISKKIFVCDRSFKVT--DLNYPSISVT--D 668
           M+PGL+YDIT DDY+ FLC+ GY    I+ I++    C  + K +  +LNYPSI+     
Sbjct: 614 MNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPT 673