Lsi07G014430 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi07G014430
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RNA-binding protein pno1
Location	chr07: 20987046 .. 20988089 (+)
RNA-Seq Expression	Lsi07G014430
Synteny	Lsi07G014430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGAACCTCTGCAAGCTTTAATACCATAGGAACTGATTTAAAAATCGCTTGTCGGATTTTCCGGATTTGAAAATGGATTATAGTATTCTAGAATCCGGTACTGTTCTAGGTTCGATGGTAGTCGTTTGTTATGAGTTAATAGTTATATATATTGGATTATAGTTTTCAAGAATCAATCCATCTTTTACTTGAATTTTTTTTTACGTTCCCAGGAAGGCGCTTCTGAAGTTTATTCAGATTTTTTATGCCTTCAATTATGCTGCCCATCGTTTTGATGGGGTATATATACACATCTTAACTGAATTTTGTTGTTTAATTGTAGTTTTTGGGTGTTTCCAGTTGTGGAAACTCAGCACAATTCCATAATGCAGTCGACCGAAGCCTCCACTTCTATGGAAGTGGAAACAATTTCAACTCAAACAAAAGATGTAGTTAGCACATTGCCACCAAAACCACAGTTTGATCCTTTAAAGCCTCATGAGATGAATGATGGTCGAGTTCAGTTCCAAAAGGTCTCGGTTCCTCCCCATCGCTATTCCCCTCTCAAGAAAGCATGGATGGAGATCTACACCCCAATTTATGATCAGATGAAAATCGACATCCGAATGAACCTCAAAGCTCGGAAAGTCGAGTTGAAAACCAGAGCAGACACACCTGATATCAGTAACTTGCAGAAGTGTGCAGATTTTGTTCATGCTTACATGCTTGGTTTTGATGTTATTGATGCCATTGCACTCTTACGAGTGGATGAACTCTATGTGGAGTCTTTTGAGATTAAAGATGTTAAAACGCTACGAGGCGAGCACTTGTCCCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACAAAATTCGCCATCGAAAACGCCACGAAGACAAGGATAGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTTGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTCATTTTAGGGTCTCCTGCTGGAAAAGTTTATTCAAAACTAAGGGCAGTTACAGCCAGATTGGCAGAAAGGTTTTGA

mRNA sequence

ATGGTTGTGGAAACTCAGCACAATTCCATAATGCAGTCGACCGAAGCCTCCACTTCTATGGAAGTGGAAACAATTTCAACTCAAACAAAAGATGTAGTTAGCACATTGCCACCAAAACCACAGTTTGATCCTTTAAAGCCTCATGAGATGAATGATGGTCGAGTTCAGTTCCAAAAGGTCTCGGTTCCTCCCCATCGCTATTCCCCTCTCAAGAAAGCATGGATGGAGATCTACACCCCAATTTATGATCAGATGAAAATCGACATCCGAATGAACCTCAAAGCTCGGAAAGTCGAGTTGAAAACCAGAGCAGACACACCTGATATCAGTAACTTGCAGAAGTGTGCAGATTTTGTTCATGCTTACATGCTTGGTTTTGATGTTATTGATGCCATTGCACTCTTACGAGTGGATGAACTCTATGTGGAGTCTTTTGAGATTAAAGATGTTAAAACGCTACGAGGCGAGCACTTGTCCCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACAAAATTCGCCATCGAAAACGCCACGAAGACAAGGATAGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTTGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTCATTTTAGGGTCTCCTGCTGGAAAAGTTTATTCAAAACTAAGGGCAGTTACAGCCAGATTGGCAGAAAGGTTTTGA

Coding sequence (CDS)

ATGGTTGTGGAAACTCAGCACAATTCCATAATGCAGTCGACCGAAGCCTCCACTTCTATGGAAGTGGAAACAATTTCAACTCAAACAAAAGATGTAGTTAGCACATTGCCACCAAAACCACAGTTTGATCCTTTAAAGCCTCATGAGATGAATGATGGTCGAGTTCAGTTCCAAAAGGTCTCGGTTCCTCCCCATCGCTATTCCCCTCTCAAGAAAGCATGGATGGAGATCTACACCCCAATTTATGATCAGATGAAAATCGACATCCGAATGAACCTCAAAGCTCGGAAAGTCGAGTTGAAAACCAGAGCAGACACACCTGATATCAGTAACTTGCAGAAGTGTGCAGATTTTGTTCATGCTTACATGCTTGGTTTTGATGTTATTGATGCCATTGCACTCTTACGAGTGGATGAACTCTATGTGGAGTCTTTTGAGATTAAAGATGTTAAAACGCTACGAGGCGAGCACTTGTCCCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACAAAATTCGCCATCGAAAACGCCACGAAGACAAGGATAGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTTGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTCATTTTAGGGTCTCCTGCTGGAAAAGTTTATTCAAAACTAAGGGCAGTTACAGCCAGATTGGCAGAAAGGTTTTGA

Protein sequence

MVVETQHNSIMQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFQKVSVPPHRYSPLKKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAYMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF

Homology

[Close]

BLAST of Lsi07G014430 vs. ExPASy Swiss-Prot
Match: Q9VR89 (RNA-binding protein pno1 OS=Drosophila melanogaster OX=7227 GN=l(1)G0004 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 3.5e-65
Identity = 120/187 (64.17%), Postives = 156/187 (83.42%), Query Frame = 0


Query: 47  PHEMNDGRVQFQKVSVPPHRYSPLKKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRADT 106
           P +    R + +KVSVPPHRYS LK+ WM+I+TP+ + MK+ IR N+KAR+VEL+   +T
Sbjct: 53  PPKAKRARSELRKVSVPPHRYSSLKEHWMKIFTPVVEHMKLQIRFNMKARQVELRVGPET 112

Query: 107 PDISNLQKCADFVHAYMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLS 166
           PDI+NLQ+ ADFV A++ GF+V DA+ALLR+++L+VESFEIKDVKTLRG+H SRAIGRL+
Sbjct: 113 PDIANLQRGADFVRAFLCGFEVDDALALLRLEDLFVESFEIKDVKTLRGDHQSRAIGRLA 172

Query: 167 GKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAV 226
           GK G+TKF IEN TKTRIV+AD+KIHILGS+ NI++AR ++C+LILGSP  KVY  LRAV
Sbjct: 173 GKGGRTKFTIENVTKTRIVLADSKIHILGSYQNIQLARRAVCNLILGSPPSKVYGNLRAV 232

Query: 227 TARLAER 234
            +RL+ER
Sbjct: 233 ASRLSER 239

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BLAST of Lsi07G014430 vs. ExPASy Swiss-Prot
Match: A7RP64 (RNA-binding protein pno1 OS=Nematostella vectensis OX=45351 GN=pno1 PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 3.0e-64
Identity = 116/196 (59.18%), Postives = 157/196 (80.10%), Query Frame = 0


Query: 39  KPQFDPLKPHEMNDGRVQFQKVSVPPHRYSPLKKAWMEIYTPIYDQMKIDIRMNLKARKV 98
           +P F P+K  ++  G+ + +K+ VP HRY+PLK+ WM+I+TP+ + +K+ IR NL +R V
Sbjct: 43  RPSFPPVKAQKLAGGKSETRKIPVPSHRYTPLKENWMKIFTPVVEHLKLQIRFNLGSRHV 102

Query: 99  ELKTRADTPDISNLQKCADFVHAYMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHL 158
           E++   +T DI  +QK ADFV A++LGF+V DA+AL+R+D+L++ESFEI DVK L+G+HL
Sbjct: 103 EIRASKETSDIGAVQKAADFVQAFILGFEVEDALALIRLDDLFLESFEIADVKPLKGDHL 162

Query: 159 SRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGK 218
           SRAIGR++GK GKTKF IEN TKTRIV+A+TKIHILGSF NIKIAR ++C+LILGSP  K
Sbjct: 163 SRAIGRVAGKGGKTKFTIENVTKTRIVLAETKIHILGSFQNIKIARTAICNLILGSPPSK 222

Query: 219 VYSKLRAVTARLAERF 235
           VY  +RAV +R AERF
Sbjct: 223 VYGNMRAVASRSAERF 238

[Close]

BLAST of Lsi07G014430 vs. ExPASy Swiss-Prot
Match: Q29IG6 (RNA-binding protein pno1 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=l(1)G0004 PE=3 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 3.9e-64
Identity = 117/178 (65.73%), Postives = 153/178 (85.96%), Query Frame = 0


Query: 56  QFQKVSVPPHRYSPLKKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKC 115
           + +KVSVPPHRYS LK+ WM+I+TP+ + MK+ IR N+KAR+VEL+   +TPDI+NLQK 
Sbjct: 60  ELRKVSVPPHRYSSLKEHWMKIFTPVVEHMKLQIRFNMKARQVELRVGPETPDIANLQKG 119

Query: 116 ADFVHAYMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFA 175
           ADFV A++ GF+V DA+ALLR+++L+VE+FEIKDVKTLRG+H SRAIGRL+GK G+TKF 
Sbjct: 120 ADFVKAFLCGFEVDDALALLRLEDLFVETFEIKDVKTLRGDHQSRAIGRLAGKGGRTKFT 179

Query: 176 IENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAER 234
           IEN TKTRIV+AD+KIHILGS+ NI++AR ++C+LILGSP  KVY  LR+V +RL+ER
Sbjct: 180 IENVTKTRIVLADSKIHILGSYQNIQLARRAICNLILGSPPSKVYGNLRSVASRLSER 237

[Close]

BLAST of Lsi07G014430 vs. ExPASy Swiss-Prot
Match: Q6VEU3 (RNA-binding protein PNO1 OS=Danio rerio OX=7955 GN=pno1 PE=2 SV=2)

HSP 1 Score: 245.0 bits (624), Expect = 8.6e-64
Identity = 121/211 (57.35%), Postives = 162/211 (76.78%), Query Frame = 0


Query: 25  ISTQTKDVVSTLPPK-PQFDPLKPHEMNDGRVQFQKVSVPPHRYSPLKKAWMEIYTPIYD 84
           + TQT++  S+ P K P F  L   ++  G  + +KV VP HRY+PLK+ W++I+TPI +
Sbjct: 42  MDTQTEESSSSAPVKRPHFPALSGDQLGGGVDEMRKVPVPSHRYTPLKENWLKIFTPIVE 101

Query: 85  QMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAYMLGFDVIDAIALLRVDELYVE 144
            +++ +R NLK R+VE+KT  +T DI  L + ADFV A++LGF V DA+AL+R+DEL++E
Sbjct: 102 NLQLQVRFNLKTRQVEIKTCKETQDIGALTRAADFVKAFVLGFQVEDALALIRLDELFLE 161

Query: 145 SFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIA 204
           +F++ DVK L+G+HLSRAIGR++GK GKTKF IEN TKTRIV+ADTKIHILGSF NIK+A
Sbjct: 162 TFDVTDVKPLKGDHLSRAIGRIAGKGGKTKFTIENVTKTRIVLADTKIHILGSFQNIKMA 221

Query: 205 RDSLCSLILGSPAGKVYSKLRAVTARLAERF 235
           R ++C+LILGSP  KVY  LRAV  R AERF
Sbjct: 222 RTAICNLILGSPPSKVYGNLRAVATRSAERF 252

[Close]

BLAST of Lsi07G014430 vs. ExPASy Swiss-Prot
Match: Q5F414 (RNA-binding protein PNO1 OS=Gallus gallus OX=9031 GN=PNO1 PE=2 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 5.6e-63
Identity = 116/196 (59.18%), Postives = 156/196 (79.59%), Query Frame = 0


Query: 39  KPQFDPLKPHEMNDGRVQFQKVSVPPHRYSPLKKAWMEIYTPIYDQMKIDIRMNLKARKV 98
           +P F PL    +  G+ + +KV VP +RY+PLK+ WM+I+TPI + +++ IR NLK R V
Sbjct: 47  RPAFPPLPAAALGVGKGEVRKVPVPANRYTPLKENWMKIFTPIVEHLQLQIRFNLKTRNV 106

Query: 99  ELKTRADTPDISNLQKCADFVHAYMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHL 158
           E+KT ++T D+S L K ADFV A++LGF V DA+AL+R+D+L++ESFE+ DVK L+G+HL
Sbjct: 107 EIKTCSETKDLSALTKAADFVKAFILGFQVEDALALIRLDDLFLESFEVTDVKPLKGDHL 166

Query: 159 SRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGK 218
           SRAIGR++GK GKTKF IEN T+TRIV+AD+KIHILGSF NIK+AR ++C+LILGSP  K
Sbjct: 167 SRAIGRIAGKGGKTKFTIENVTRTRIVLADSKIHILGSFQNIKMARTAICNLILGSPPSK 226

Query: 219 VYSKLRAVTARLAERF 235
           VY  +RAV +R AERF
Sbjct: 227 VYGNIRAVASRAAERF 242

[Close]

BLAST of Lsi07G014430 vs. ExPASy TrEMBL
Match: A0A1S3BLP2 (RNA-binding protein pno1 OS=Cucumis melo OX=3656 GN=LOC103491005 PE=3 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 4.1e-117
Identity = 221/224 (98.66%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 11  MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFQKVSVPPHRYSPL 70
           MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQF+KVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 71  KKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAYMLGFDVID 130
           KKAWMEIYTPIYDQMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHA+MLGFDVID
Sbjct: 61  KKAWMEIYTPIYDQMKIDVRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 131 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 190
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 191 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 235
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Lsi07G014430 vs. ExPASy TrEMBL
Match: A0A6J1EWL7 (RNA-binding protein pno1 OS=Cucurbita moschata OX=3662 GN=LOC111438772 PE=3 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 2.9e-115
Identity = 216/224 (96.43%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 11  MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFQKVSVPPHRYSPL 70
           MQSTEASTSMEVET+ST+TK+VVSTLPPKPQFDPLKPHEMNDGR+QF+KVSVPPHRY+PL
Sbjct: 1   MQSTEASTSMEVETVSTETKNVVSTLPPKPQFDPLKPHEMNDGRIQFRKVSVPPHRYTPL 60

Query: 71  KKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAYMLGFDVID 130
           KKAWMEIYTPIY+QMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHA+MLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 131 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 190
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 191 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 235
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Lsi07G014430 vs. ExPASy TrEMBL
Match: A0A0A0L7D9 (KH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G045150 PE=3 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 8.5e-115
Identity = 218/224 (97.32%), Postives = 222/224 (99.11%), Query Frame = 0


Query: 11  MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFQKVSVPPHRYSPL 70
           MQS EASTSM+VETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQF+KVSVPPHRYSPL
Sbjct: 1   MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 71  KKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAYMLGFDVID 130
           KKAWM+IYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQK ADFVHA+MLGFDVID
Sbjct: 61  KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 120

Query: 131 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 190
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 191 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 235
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Lsi07G014430 vs. ExPASy TrEMBL
Match: A0A6J1HQ98 (RNA-binding protein pno1-like OS=Cucurbita maxima OX=3661 GN=LOC111465109 PE=3 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 3.2e-114
Identity = 214/224 (95.54%), Postives = 223/224 (99.55%), Query Frame = 0


Query: 11  MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFQKVSVPPHRYSPL 70
           MQ TEASTSMEVET+ST+TK+VVSTLPPKPQFDPLKPHEMNDGR+QF+KVSVPPHRY+PL
Sbjct: 1   MQPTEASTSMEVETVSTETKNVVSTLPPKPQFDPLKPHEMNDGRIQFRKVSVPPHRYTPL 60

Query: 71  KKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAYMLGFDVID 130
           KKAWMEIYTPIY+QMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHA++LGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFILGFDVID 120

Query: 131 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 190
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 191 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 235
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Lsi07G014430 vs. ExPASy TrEMBL
Match: A0A6J1KXN9 (RNA-binding protein pno1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498497 PE=3 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 5.5e-114
Identity = 216/224 (96.43%), Postives = 220/224 (98.21%), Query Frame = 0


Query: 11  MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFQKVSVPPHRYSPL 70
           MQSTEASTSMEVE +STQ K VVSTLPPKP FDPLKPHEMNDGRVQF+KVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 71  KKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAYMLGFDVID 130
           KKAWMEIYTPIY+QMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHA+MLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 131 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 190
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 191 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 235
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Lsi07G014430 vs. NCBI nr
Match: KAG6577533.1 (Gamma-tubulin complex component 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 436.8 bits (1122), Expect = 1.2e-118
Identity = 222/232 (95.69%), Postives = 231/232 (99.57%), Query Frame = 0


Query: 3   VETQHNSIMQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFQKVSV 62
           VETQHNSIMQSTE+STSMEVET+ST+TK+VVSTLPPKPQFDPLKPHEMNDGR+QF+KVSV
Sbjct: 702 VETQHNSIMQSTESSTSMEVETVSTETKNVVSTLPPKPQFDPLKPHEMNDGRIQFRKVSV 761

Query: 63  PPHRYSPLKKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAY 122
           PPHRY+PLKKAWMEIYTPIY+QMKIDIRMNLK RKVELKTRADTPDISNLQKCADFVHA+
Sbjct: 762 PPHRYTPLKKAWMEIYTPIYEQMKIDIRMNLKVRKVELKTRADTPDISNLQKCADFVHAF 821

Query: 123 MLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKT 182
           MLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKT
Sbjct: 822 MLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKT 881

Query: 183 RIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 235
           RIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 882 RIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 933

[Close]

BLAST of Lsi07G014430 vs. NCBI nr
Match: KAE8650098.1 (hypothetical protein Csa_010636 [Cucumis sativus])

HSP 1 Score: 431.0 bits (1107), Expect = 6.5e-117
Identity = 225/233 (96.57%), Postives = 229/233 (98.28%), Query Frame = 0


Query: 3   VETQHN-SIMQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFQKVS 62
           VET HN SIMQS EASTSM+VETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQF+KVS
Sbjct: 196 VETPHNYSIMQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVS 255

Query: 63  VPPHRYSPLKKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHA 122
           VPPHRYSPLKKAWM+IYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQK ADFVHA
Sbjct: 256 VPPHRYSPLKKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHA 315

Query: 123 YMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATK 182
           +MLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATK
Sbjct: 316 FMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATK 375

Query: 183 TRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 235
           TRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 376 TRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 428

[Close]

BLAST of Lsi07G014430 vs. NCBI nr
Match: XP_008449001.1 (PREDICTED: RNA-binding protein pno1 [Cucumis melo])

HSP 1 Score: 430.6 bits (1106), Expect = 8.4e-117
Identity = 221/224 (98.66%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 11  MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFQKVSVPPHRYSPL 70
           MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQF+KVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 71  KKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAYMLGFDVID 130
           KKAWMEIYTPIYDQMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHA+MLGFDVID
Sbjct: 61  KKAWMEIYTPIYDQMKIDVRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 131 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 190
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 191 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 235
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Lsi07G014430 vs. NCBI nr
Match: XP_022932417.1 (RNA-binding protein pno1 [Cucurbita moschata])

HSP 1 Score: 424.5 bits (1090), Expect = 6.0e-115
Identity = 216/224 (96.43%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 11  MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFQKVSVPPHRYSPL 70
           MQSTEASTSMEVET+ST+TK+VVSTLPPKPQFDPLKPHEMNDGR+QF+KVSVPPHRY+PL
Sbjct: 1   MQSTEASTSMEVETVSTETKNVVSTLPPKPQFDPLKPHEMNDGRIQFRKVSVPPHRYTPL 60

Query: 71  KKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAYMLGFDVID 130
           KKAWMEIYTPIY+QMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHA+MLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 131 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 190
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 191 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 235
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Lsi07G014430 vs. NCBI nr
Match: KAG7015597.1 (RNA-binding protein pno1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 423.3 bits (1087), Expect = 1.3e-114
Identity = 215/224 (95.98%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 11  MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFQKVSVPPHRYSPL 70
           MQSTE+STSMEVET+ST+TK+VVSTLPPKPQFDPLKPHEMNDGR+QF+KVSVPPHRY+PL
Sbjct: 1   MQSTESSTSMEVETVSTETKNVVSTLPPKPQFDPLKPHEMNDGRIQFRKVSVPPHRYTPL 60

Query: 71  KKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAYMLGFDVID 130
           KKAWMEIYTPIY+QMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHA+MLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 131 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 190
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 191 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 235
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Lsi07G014430 vs. TAIR 10
Match: AT3G13230.1 (RNA-binding KH domain-containing protein )

HSP 1 Score: 346.7 bits (888), Expect = 1.5e-95
Identity = 175/216 (81.02%), Postives = 198/216 (91.67%), Query Frame = 0


Query: 17  STSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFQKVSVPPHRYSPLKKAWME 76
           ST MEVET +  T      LPPKP F PLK HEM+DG+VQF+K++VPP+RYSPLKKAW++
Sbjct: 4   STQMEVETATEGT----VPLPPKPTFKPLKAHEMSDGKVQFRKIAVPPNRYSPLKKAWLD 63

Query: 77  IYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAYMLGFDVIDAIALLR 136
           IYTPIYDQMK+DIRMNLKARKVELKTRADTPDISNLQK ADFVHA+MLGFD+ DAI+LLR
Sbjct: 64  IYTPIYDQMKVDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDIPDAISLLR 123

Query: 137 VDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGS 196
           +DELYVESFEIKDVKTL+GEHLSRAIGRLSGK GKTKFAIEN+TKTRIVIADT+IHILG+
Sbjct: 124 MDELYVESFEIKDVKTLKGEHLSRAIGRLSGKGGKTKFAIENSTKTRIVIADTRIHILGA 183

Query: 197 FANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 233
           F+NIK+AR SLCSLI+GSPAGKVYSKLR+V+ARL E
Sbjct: 184 FSNIKVARSSLCSLIMGSPAGKVYSKLRSVSARLNE 215

The following BLAST results are available for this feature:

BLAST of Lsi07G014430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9VR89	3.5e-65	64.17	RNA-binding protein pno1 OS=Drosophila melanogaster OX=7227 GN=l(1)G0004 PE=2 SV...	[more]
A7RP64	3.0e-64	59.18	RNA-binding protein pno1 OS=Nematostella vectensis OX=45351 GN=pno1 PE=3 SV=1	[more]
Q29IG6	3.9e-64	65.73	RNA-binding protein pno1 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=l...	[more]
Q6VEU3	8.6e-64	57.35	RNA-binding protein PNO1 OS=Danio rerio OX=7955 GN=pno1 PE=2 SV=2	[more]
Q5F414	5.6e-63	59.18	RNA-binding protein PNO1 OS=Gallus gallus OX=9031 GN=PNO1 PE=2 SV=1	[more]

BLAST of Lsi07G014430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BLP2	4.1e-117	98.66	RNA-binding protein pno1 OS=Cucumis melo OX=3656 GN=LOC103491005 PE=3 SV=1	[more]
A0A6J1EWL7	2.9e-115	96.43	RNA-binding protein pno1 OS=Cucurbita moschata OX=3662 GN=LOC111438772 PE=3 SV=1	[more]
A0A0A0L7D9	8.5e-115	97.32	KH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G045150 PE=3 SV=...	[more]
A0A6J1HQ98	3.2e-114	95.54	RNA-binding protein pno1-like OS=Cucurbita maxima OX=3661 GN=LOC111465109 PE=3 S...	[more]
A0A6J1KXN9	5.5e-114	96.43	RNA-binding protein pno1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]

BLAST of Lsi07G014430 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6577533.1	1.2e-118	95.69	Gamma-tubulin complex component 2, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAE8650098.1	6.5e-117	96.57	hypothetical protein Csa_010636 [Cucumis sativus]	[more]
XP_008449001.1	8.4e-117	98.66	PREDICTED: RNA-binding protein pno1 [Cucumis melo]	[more]
XP_022932417.1	6.0e-115	96.43	RNA-binding protein pno1 [Cucurbita moschata]	[more]
KAG7015597.1	1.3e-114	95.98	RNA-binding protein pno1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Lsi07G014430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13230.1	1.5e-95	81.02	RNA-binding KH domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004087	K Homology domain	SMART	SM00322	kh_6	coord: 139..212 e-value: 1.7E-4 score: 31.0
IPR036612	K Homology domain, type 1 superfamily	GENE3D	3.30.1370.10	K Homology domain, type 1	coord: 138..234 e-value: 1.2E-22 score: 81.8
IPR036612	K Homology domain, type 1 superfamily	SUPERFAMILY	54791	Eukaryotic type KH-domain (KH-domain type I)	coord: 139..233
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 28..48
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR12826	RIBONUCLEASE Y	coord: 17..234
IPR039912	Pre-rRNA-processing protein PNO1-like	PANTHER	PTHR12826:SF13	RNA-BINDING PROTEIN PNO1	coord: 17..234

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi07G014430.1	Lsi07G014430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003676	nucleic acid binding