Lsi07G013500 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi07G013500
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	methylsterol monooxygenase 1-1-like
Location	chr07: 19671980 .. 19673626 (-)
RNA-Seq Expression	Lsi07G013500
Synteny	Lsi07G013500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGGTGTCAGACAATCATTTCTTTGGTCATATAGGACATAGCTTCACTCTTGGTGGGTTTCCTGTGGCTGAACATCCCCTTAGCCTTTTGTTGAACTTGAATAACTTTATATATTATTATTTTATATTGCTGAAGTTTGAATGTTGTCTCTTTCATACAACGACTGAGATTCGCATGAACATTGATGGCAAGATGTTGTACAACTTTTGATGCTAGCAAAAATGCCAAAAAAAAATTCAAAATTGAAGTCTTCTATTCACAACAGTGAAGTTGAAGGAATTTTTTTATCAATATATTTCACCAGCATTGGTGTCAATGAAACTATCACCTCTTCATTCTTAATTGATTTTAATGTTAGTTTAAATTGCCATTATAATTCTTATATTTTGAGCTTGATTTCAGCTTGGTCATCGTAGTTATATAGTTTAATTATCATCTTTCGTCACTGGATATTAATAGTTTTCTTATCTGTCATGTTTTCAATGGACGTGATAGAGAGGAGTGATGAGTTGATGTTTAGTATAAATCATGTGTGAAGGTTAAGAGGATAAGCCTTACTGCTTGTCTTCAATAATCCTAATCTCCAAAATCCACTATTCTCTCTACCGCGTCCATAGAAACTTACTAGTAGAGACGTATTGGGAGGTATAACCAAATTATAGGGACTTCAATAAACTTTTGAGAACAAATTGAAACCGAGCACAAACCATAGGGACCAAAATGGTAATATGACCTTATTTTTGTTACAAATAAACTATTCATGAGAATTACATCATTCCTCTTGTTCTTTAGTGTGGTCTTTTTTTTTACCTCTGCTTTTTCTCTCAGATTGTGCTTTTATGATGATTGTTGAGGTCATCCCTGGTTTTATTCACATATTTTATATTTATATTCAGGTATGATTTCCCTTGGAGTCTAACGAAATTTATACCATTTTATGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGGCAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGTAGTTATGGTCTTGTTATAATTTTACCTCCCACTTGTTCTTCCAGTAAAATCATGCTCATAAAAGTTACTCATTTGTTTACTTCCTCAGGGGTATCGCTATCAAAAGAAGATCCTTAAGAAGGTAAGCGTTTTGCTTTTCCCTGCCCCCACCATCGCCCGAAAATTGATTGTATATGGTGTCATGAATTTGCATTAGTTTCAAGTTTTCTTCAAATGGACATTAGATTTAGCCAAAATTGAACTTGGTTCGATGGCATGGCCGATAAGCCTTAAGATGGCTAGTTTTTAACCCCATATTTACTATGCCTTTCTCCTGTTCTGTTTGGTTGGATCAAATCTATGCCATTTTTTATTTTGGTCATCACTCCTCAGCACCTGTTCCATTTTTCATTTTGCTTTGTTTTGGAGGGAGGAGAGGTCTGACTGTGAATGTAAAAGAAAAAGAAAGGACAATTTATGACTGCTACTCTTTTTATCGGGTTTCGTATCGTTAACGATACCTCGTGTTCTAATTGTTTGTTGTTGATCAGTTGAAGGAAGAAGTGAAGAACAGTGAAGAGTCATACTACAGCACAGTCCAAGATGTTAAATCGGACTAG

mRNA sequence

ATGCGGTATGATTTCCCTTGGAGTCTAACGAAATTTATACCATTTTATGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGGCAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGGGTATCGCTATCAAAAGAAGATCCTTAAGAAGGTAAGCGTTTTGCTTTTCCCTGCCCCCACCATCGCCCGAAAATTGATTTTGAAGGAAGAAGTGAAGAACAGTGAAGAGTCATACTACAGCACAGTCCAAGATGTTAAATCGGACTAG

Coding sequence (CDS)

ATGCGGTATGATTTCCCTTGGAGTCTAACGAAATTTATACCATTTTATGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGGCAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGGGTATCGCTATCAAAAGAAGATCCTTAAGAAGGTAAGCGTTTTGCTTTTCCCTGCCCCCACCATCGCCCGAAAATTGATTTTGAAGGAAGAAGTGAAGAACAGTGAAGAGTCATACTACAGCACAGTCCAAGATGTTAAATCGGACTAG

Protein sequence

MRYDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKVSVLLFPAPTIARKLILKEEVKNSEESYYSTVQDVKSD

Homology

[Close]

BLAST of Lsi07G013500 vs. ExPASy Swiss-Prot
Match: Q1EC69 (Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana OX=3702 GN=SMO1-2 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.2e-27
Identity = 52/61 (85.25%), Postives = 60/61 (98.36%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 62
           YDFPWSLTK+IPFYGGA++HDYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L++
Sbjct: 221 YDFPWSLTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQ 280

Query: 63  V 64
           +
Sbjct: 281 M 281

[Close]

BLAST of Lsi07G013500 vs. ExPASy Swiss-Prot
Match: Q8L7W5 (Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana OX=3702 GN=SMO1-1 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 4.7e-27
Identity = 51/61 (83.61%), Postives = 59/61 (96.72%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 62
           YDFPWS TK+IPFYGGA++HDYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L++
Sbjct: 221 YDFPWSPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQ 280

Query: 63  V 64
           +
Sbjct: 281 I 281

[Close]

BLAST of Lsi07G013500 vs. ExPASy Swiss-Prot
Match: F4JLZ6 (Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana OX=3702 GN=SMO1-3 PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 4.4e-25
Identity = 49/58 (84.48%), Postives = 53/58 (91.38%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL 61
           YDFPWS+TK IPFYGG ++HDYHHYVGGQS SNFASVFTYCDYIYGTDKGYR  KK+L
Sbjct: 217 YDFPWSVTKLIPFYGGPEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLL 274

[Close]

BLAST of Lsi07G013500 vs. ExPASy Swiss-Prot
Match: Q9AV39 (Very-long-chain aldehyde decarbonylase GL1-10 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-10 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 2.4e-23
Identity = 47/64 (73.44%), Postives = 55/64 (85.94%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 62
           +DFP++LTK+IPFYGGA++HDYHHYVG QS SNFASVFTYCDY+YGTDKGYRY K    K
Sbjct: 219 FDFPFNLTKYIPFYGGAEYHDYHHYVGRQSQSNFASVFTYCDYLYGTDKGYRYHKAYQAK 278

Query: 63  VSVL 67
           +  L
Sbjct: 279 MKAL 282

[Close]

BLAST of Lsi07G013500 vs. ExPASy Swiss-Prot
Match: Q8VWZ8 (Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 3.0e-13
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKI 60
           Y FPWSL+ F+P YGGAD HDYHH +      N++S F Y D+I+GTDKGYR  K +
Sbjct: 201 YHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKTL 257

[Close]

BLAST of Lsi07G013500 vs. ExPASy TrEMBL
Match: A0A0A0L5P8 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G036480 PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 3.4e-36
Identity = 78/98 (79.59%), Postives = 81/98 (82.65%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 62
           YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+K
Sbjct: 219 YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQK 278

Query: 63  VSVLLFPAPTIARKLILKEEVKNSEESYYSTVQDVKSD 101
                           LKEEVKNSEESYY+T Q+VKSD
Sbjct: 279 ----------------LKEEVKNSEESYYNTAQNVKSD 300

[Close]

BLAST of Lsi07G013500 vs. ExPASy TrEMBL
Match: A0A6J1HQ57 (methylsterol monooxygenase 1-2 OS=Cucurbita maxima OX=3661 GN=LOC111465081 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.7e-35
Identity = 77/98 (78.57%), Postives = 79/98 (80.61%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 62
           YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK
Sbjct: 219 YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 278

Query: 63  VSVLLFPAPTIARKLILKEEVKNSEESYYSTVQDVKSD 101
                           LKEEVK+SE SYYST +DVK D
Sbjct: 279 ----------------LKEEVKSSEGSYYSTAEDVKVD 300

[Close]

BLAST of Lsi07G013500 vs. ExPASy TrEMBL
Match: A0A5D3D7N8 (Methylsterol monooxygenase 1-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001250 PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 8.3e-35
Identity = 78/99 (78.79%), Postives = 81/99 (81.82%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 62
           YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+K
Sbjct: 129 YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQK 188

Query: 63  VSVLLFPAPTIARKLILKEEVKNSEE-SYYSTVQDVKSD 101
                           LKEEVKNSEE SYY+T Q+VKSD
Sbjct: 189 ----------------LKEEVKNSEESSYYNTAQNVKSD 211

[Close]

BLAST of Lsi07G013500 vs. ExPASy TrEMBL
Match: A0A6J1E9U9 (methylsterol monooxygenase 1-2 OS=Cucurbita moschata OX=3662 GN=LOC111431186 PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 8.3e-35
Identity = 76/98 (77.55%), Postives = 79/98 (80.61%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 62
           YDFPWSLTKFIPFYGG+DHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK
Sbjct: 219 YDFPWSLTKFIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 278

Query: 63  VSVLLFPAPTIARKLILKEEVKNSEESYYSTVQDVKSD 101
                           LKEEVK+SE SYYST +DVK D
Sbjct: 279 ----------------LKEEVKSSEGSYYSTAEDVKVD 300

[Close]

BLAST of Lsi07G013500 vs. ExPASy TrEMBL
Match: A0A1S3BKU6 (methylsterol monooxygenase 1-2-like OS=Cucumis melo OX=3656 GN=LOC103490939 PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 8.3e-35
Identity = 78/99 (78.79%), Postives = 81/99 (81.82%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 62
           YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+K
Sbjct: 219 YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQK 278

Query: 63  VSVLLFPAPTIARKLILKEEVKNSEE-SYYSTVQDVKSD 101
                           LKEEVKNSEE SYY+T Q+VKSD
Sbjct: 279 ----------------LKEEVKNSEESSYYNTAQNVKSD 301

[Close]

BLAST of Lsi07G013500 vs. NCBI nr
Match: XP_011650426.1 (methylsterol monooxygenase 1-2 [Cucumis sativus] >KGN55922.1 hypothetical protein Csa_009711 [Cucumis sativus])

HSP 1 Score: 160.6 bits (405), Expect = 7.0e-36
Identity = 78/98 (79.59%), Postives = 81/98 (82.65%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 62
           YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+K
Sbjct: 219 YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQK 278

Query: 63  VSVLLFPAPTIARKLILKEEVKNSEESYYSTVQDVKSD 101
                           LKEEVKNSEESYY+T Q+VKSD
Sbjct: 279 ----------------LKEEVKNSEESYYNTAQNVKSD 300

[Close]

BLAST of Lsi07G013500 vs. NCBI nr
Match: XP_023552163.1 (methylsterol monooxygenase 1-2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 157.1 bits (396), Expect = 7.7e-35
Identity = 77/98 (78.57%), Postives = 79/98 (80.61%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 62
           YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK
Sbjct: 219 YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 278

Query: 63  VSVLLFPAPTIARKLILKEEVKNSEESYYSTVQDVKSD 101
                           LKEEVK+SE SYYST +DVK D
Sbjct: 279 ----------------LKEEVKSSEGSYYSTAEDVKVD 300

[Close]

BLAST of Lsi07G013500 vs. NCBI nr
Match: XP_022965134.1 (methylsterol monooxygenase 1-2 [Cucurbita maxima])

HSP 1 Score: 157.1 bits (396), Expect = 7.7e-35
Identity = 77/98 (78.57%), Postives = 79/98 (80.61%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 62
           YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK
Sbjct: 219 YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 278

Query: 63  VSVLLFPAPTIARKLILKEEVKNSEESYYSTVQDVKSD 101
                           LKEEVK+SE SYYST +DVK D
Sbjct: 279 ----------------LKEEVKSSEGSYYSTAEDVKVD 300

[Close]

BLAST of Lsi07G013500 vs. NCBI nr
Match: KAG7028362.1 (Methylsterol monooxygenase 1-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 156.4 bits (394), Expect = 1.3e-34
Identity = 77/99 (77.78%), Postives = 78/99 (78.79%), Query Frame = 0


Query: 2   RYDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILK 61
           RYDFPWS+TK IPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILK
Sbjct: 220 RYDFPWSVTKLIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILK 279

Query: 62  KVSVLLFPAPTIARKLILKEEVKNSEESYYSTVQDVKSD 101
           K                LKEE K SE SYYST QDVKSD
Sbjct: 280 K----------------LKEEAKISEASYYSTAQDVKSD 302

[Close]

BLAST of Lsi07G013500 vs. NCBI nr
Match: TYK19470.1 (methylsterol monooxygenase 1-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 156.0 bits (393), Expect = 1.7e-34
Identity = 78/99 (78.79%), Postives = 81/99 (81.82%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 62
           YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+K
Sbjct: 129 YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQK 188

Query: 63  VSVLLFPAPTIARKLILKEEVKNSEE-SYYSTVQDVKSD 101
                           LKEEVKNSEE SYY+T Q+VKSD
Sbjct: 189 ----------------LKEEVKNSEESSYYNTAQNVKSD 211

[Close]

BLAST of Lsi07G013500 vs. TAIR 10
Match: AT4G22756.1 (sterol C4-methyl oxidase 1-2 )

HSP 1 Score: 123.6 bits (309), Expect = 8.8e-29
Identity = 52/61 (85.25%), Postives = 60/61 (98.36%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 62
           YDFPWSLTK+IPFYGGA++HDYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L++
Sbjct: 221 YDFPWSLTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQ 280

Query: 63  V 64
           +
Sbjct: 281 M 281

[Close]

BLAST of Lsi07G013500 vs. TAIR 10
Match: AT4G12110.1 (sterol-4alpha-methyl oxidase 1-1 )

HSP 1 Score: 121.7 bits (304), Expect = 3.3e-28
Identity = 51/61 (83.61%), Postives = 59/61 (96.72%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKK 62
           YDFPWS TK+IPFYGGA++HDYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L++
Sbjct: 221 YDFPWSPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQ 280

Query: 63  V 64
           +
Sbjct: 281 I 281

[Close]

BLAST of Lsi07G013500 vs. TAIR 10
Match: AT4G22753.1 (sterol 4-alpha methyl oxidase 1-3 )

HSP 1 Score: 115.2 bits (287), Expect = 3.1e-26
Identity = 49/58 (84.48%), Postives = 53/58 (91.38%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL 61
           YDFPWS+TK IPFYGG ++HDYHHYVGGQS SNFASVFTYCDYIYGTDKGYR  KK+L
Sbjct: 217 YDFPWSVTKLIPFYGGPEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLL 274

[Close]

BLAST of Lsi07G013500 vs. TAIR 10
Match: AT4G22753.2 (sterol 4-alpha methyl oxidase 1-3 )

HSP 1 Score: 115.2 bits (287), Expect = 3.1e-26
Identity = 49/58 (84.48%), Postives = 53/58 (91.38%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL 61
           YDFPWS+TK IPFYGG ++HDYHHYVGGQS SNFASVFTYCDYIYGTDKGYR  KK+L
Sbjct: 217 YDFPWSVTKLIPFYGGPEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLL 274

[Close]

BLAST of Lsi07G013500 vs. TAIR 10
Match: AT1G07420.1 (sterol 4-alpha-methyl-oxidase 2-1 )

HSP 1 Score: 75.9 bits (185), Expect = 2.1e-14
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 0


Query: 3   YDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKI 60
           Y FPWSL+ F+P YGGAD HDYHH +      N++S F Y D+I+GTDKGYR  K +
Sbjct: 201 YHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKTL 257

The following BLAST results are available for this feature:

BLAST of Lsi07G013500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1EC69	1.2e-27	85.25	Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana OX=3702 GN=SMO1-2 PE=2 SV...	[more]
Q8L7W5	4.7e-27	83.61	Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana OX=3702 GN=SMO1-1 PE=1 SV...	[more]
F4JLZ6	4.4e-25	84.48	Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana OX=3702 GN=SMO1-3 PE=2 SV...	[more]
Q9AV39	2.4e-23	73.44	Very-long-chain aldehyde decarbonylase GL1-10 OS=Oryza sativa subsp. japonica OX...	[more]
Q8VWZ8	3.0e-13	56.14	Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV...	[more]

BLAST of Lsi07G013500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5P8	3.4e-36	79.59	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A6J1HQ57	3.7e-35	78.57	methylsterol monooxygenase 1-2 OS=Cucurbita maxima OX=3661 GN=LOC111465081 PE=3 ...	[more]
A0A5D3D7N8	8.3e-35	78.79	Methylsterol monooxygenase 1-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A6J1E9U9	8.3e-35	77.55	methylsterol monooxygenase 1-2 OS=Cucurbita moschata OX=3662 GN=LOC111431186 PE=...	[more]
A0A1S3BKU6	8.3e-35	78.79	methylsterol monooxygenase 1-2-like OS=Cucumis melo OX=3656 GN=LOC103490939 PE=3...	[more]

BLAST of Lsi07G013500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650426.1	7.0e-36	79.59	methylsterol monooxygenase 1-2 [Cucumis sativus] >KGN55922.1 hypothetical protei...	[more]
XP_023552163.1	7.7e-35	78.57	methylsterol monooxygenase 1-2 [Cucurbita pepo subsp. pepo]	[more]
XP_022965134.1	7.7e-35	78.57	methylsterol monooxygenase 1-2 [Cucurbita maxima]	[more]
KAG7028362.1	1.3e-34	77.78	Methylsterol monooxygenase 1-2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
TYK19470.1	1.7e-34	78.79	methylsterol monooxygenase 1-2-like [Cucumis melo var. makuwa]	[more]

BLAST of Lsi07G013500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G22756.1	8.8e-29	85.25	sterol C4-methyl oxidase 1-2	[more]
AT4G12110.1	3.3e-28	83.61	sterol-4alpha-methyl oxidase 1-1	[more]
AT4G22753.1	3.1e-26	84.48	sterol 4-alpha methyl oxidase 1-3	[more]
AT4G22753.2	3.1e-26	84.48	sterol 4-alpha methyl oxidase 1-3	[more]
AT1G07420.1	2.1e-14	56.14	sterol 4-alpha-methyl-oxidase 2-1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR11863:SF180	METHYLSTEROL MONOOXYGENASE 1-1	coord: 3..65
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 3..65

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi07G013500.1	Lsi07G013500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016126	sterol biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0000254	C-4 methylsterol oxidase activity
molecular_function	GO:0005506	iron ion binding