Lsi07G012740 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G012740
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionethylene-responsive transcription factor 5
Locationchr07: 18569260 .. 18570273 (+)
RNA-Seq ExpressionLsi07G012740
SyntenyLsi07G012740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTTGTGAATCTTGAAGAAGCTGCTGCGTTGGAGAATATTAAGCTTCATCTTCTCGGCGACCTCTCTCCTTTGCCGAGGATTTTGGCTTCTGATAACCTTTGCGTTTCATCATCGGATCGTTCGGTGAGTTCTGATTCTTCATCGACCTCGAACTGTGTTCTTAACATCTTTGATTACTTCGATTCCGATGAAATCTTCGAATTCTCTGATTTATCATCGTCTATGTCATCAAACGACGATTTCTTTGGATTTGAGATGAAACCTAACGTTATTGACCTAACAACGCCGAAATCGACTGAATTACTCGAATTCGAGACGAAACCTACGGTTTTCGAGGATTTGAGTTCGAGGACTCTGTACAGTTGTAATTCGTTTGAAGTTGAATCGAAGATTAGTCAGGTAACGAATTCGAATCGGAAACGAGCGAATTTGAAAATCTCGCTTCCGAATAAGTCTACGCAGTGGATTAATTTTGATTCGGCGGCGGAGAAGAAGACTCCGGTTGTTGTACAGAGGAGTAAAGATCTTGAGGCGGAGAAGAAAGTTCATTACAGAGGTGTTCGACAAAGGCCTTGGGGAAAATTCGCCGCCGAGATTAGGGATCCGAATCGTCGTGGATCTAGAGTTTGGCTTGGAACTTTTGAGACTGCAATCGAAGCGGCGAGAGCGTACGATCGCGCTGCTTTCAAGCTTCGTGGATCTAAAGCGATTCTTAACTTTCCGTTGGAAGCTGCGAACTCTTACTCGGAACCGGTCGCCGCCGGGAAGCGGCGGAGGGAGGAGGAAGTTGAAGAGGTGGTTGAGGCGGTGGCGGTTAAAAAAGAGAGGACGGCGGAGGAGGTGAATCCAGCCGCTGCCGACGTTGGTTACTTGAAAGATATGCCGTTAACGCCGTCGTCTTGGTCGATGGTTTGGGACGGTGAAATGAAAGGACTTTTCAATATTCCACCGTTATCGCCGTTATCTCCTCATCCTGCATTTGGATTTCCGCAGCTTATGGTTGTATAA

mRNA sequence

ATGGAATTTGTGAATCTTGAAGAAGCTGCTGCGTTGGAGAATATTAAGCTTCATCTTCTCGGCGACCTCTCTCCTTTGCCGAGGATTTTGGCTTCTGATAACCTTTGCGTTTCATCATCGGATCGTTCGGTGAGTTCTGATTCTTCATCGACCTCGAACTGTGTTCTTAACATCTTTGATTACTTCGATTCCGATGAAATCTTCGAATTCTCTGATTTATCATCGTCTATGTCATCAAACGACGATTTCTTTGGATTTGAGATGAAACCTAACGTTATTGACCTAACAACGCCGAAATCGACTGAATTACTCGAATTCGAGACGAAACCTACGGTTTTCGAGGATTTGAGTTCGAGGACTCTGTACAGTTGTAATTCGTTTGAAGTTGAATCGAAGATTAGTCAGGTAACGAATTCGAATCGGAAACGAGCGAATTTGAAAATCTCGCTTCCGAATAAGTCTACGCAGTGGATTAATTTTGATTCGGCGGCGGAGAAGAAGACTCCGGTTGTTGTACAGAGGAGTAAAGATCTTGAGGCGGAGAAGAAAGTTCATTACAGAGGTGTTCGACAAAGGCCTTGGGGAAAATTCGCCGCCGAGATTAGGGATCCGAATCGTCGTGGATCTAGAGTTTGGCTTGGAACTTTTGAGACTGCAATCGAAGCGGCGAGAGCGTACGATCGCGCTGCTTTCAAGCTTCGTGGATCTAAAGCGATTCTTAACTTTCCGTTGGAAGCTGCGAACTCTTACTCGGAACCGGTCGCCGCCGGGAAGCGGCGGAGGGAGGAGGAAGTTGAAGAGGTGGTTGAGGCGGTGGCGGTTAAAAAAGAGAGGACGGCGGAGGAGGTGAATCCAGCCGCTGCCGACGTTGGTTACTTGAAAGATATGCCGTTAACGCCGTCGTCTTGGTCGATGGTTTGGGACGGTGAAATGAAAGGACTTTTCAATATTCCACCGTTATCGCCGTTATCTCCTCATCCTGCATTTGGATTTCCGCAGCTTATGGTTGTATAA

Coding sequence (CDS)

ATGGAATTTGTGAATCTTGAAGAAGCTGCTGCGTTGGAGAATATTAAGCTTCATCTTCTCGGCGACCTCTCTCCTTTGCCGAGGATTTTGGCTTCTGATAACCTTTGCGTTTCATCATCGGATCGTTCGGTGAGTTCTGATTCTTCATCGACCTCGAACTGTGTTCTTAACATCTTTGATTACTTCGATTCCGATGAAATCTTCGAATTCTCTGATTTATCATCGTCTATGTCATCAAACGACGATTTCTTTGGATTTGAGATGAAACCTAACGTTATTGACCTAACAACGCCGAAATCGACTGAATTACTCGAATTCGAGACGAAACCTACGGTTTTCGAGGATTTGAGTTCGAGGACTCTGTACAGTTGTAATTCGTTTGAAGTTGAATCGAAGATTAGTCAGGTAACGAATTCGAATCGGAAACGAGCGAATTTGAAAATCTCGCTTCCGAATAAGTCTACGCAGTGGATTAATTTTGATTCGGCGGCGGAGAAGAAGACTCCGGTTGTTGTACAGAGGAGTAAAGATCTTGAGGCGGAGAAGAAAGTTCATTACAGAGGTGTTCGACAAAGGCCTTGGGGAAAATTCGCCGCCGAGATTAGGGATCCGAATCGTCGTGGATCTAGAGTTTGGCTTGGAACTTTTGAGACTGCAATCGAAGCGGCGAGAGCGTACGATCGCGCTGCTTTCAAGCTTCGTGGATCTAAAGCGATTCTTAACTTTCCGTTGGAAGCTGCGAACTCTTACTCGGAACCGGTCGCCGCCGGGAAGCGGCGGAGGGAGGAGGAAGTTGAAGAGGTGGTTGAGGCGGTGGCGGTTAAAAAAGAGAGGACGGCGGAGGAGGTGAATCCAGCCGCTGCCGACGTTGGTTACTTGAAAGATATGCCGTTAACGCCGTCGTCTTGGTCGATGGTTTGGGACGGTGAAATGAAAGGACTTTTCAATATTCCACCGTTATCGCCGTTATCTCCTCATCCTGCATTTGGATTTCCGCAGCTTATGGTTGTATAA

Protein sequence

MEFVNLEEAAALENIKLHLLGDLSPLPRILASDNLCVSSSDRSVSSDSSSTSNCVLNIFDYFDSDEIFEFSDLSSSMSSNDDFFGFEMKPNVIDLTTPKSTELLEFETKPTVFEDLSSRTLYSCNSFEVESKISQVTNSNRKRANLKISLPNKSTQWINFDSAAEKKTPVVVQRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFKLRGSKAILNFPLEAANSYSEPVAAGKRRREEEVEEVVEAVAVKKERTAEEVNPAAADVGYLKDMPLTPSSWSMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMVV
Homology
BLAST of Lsi07G012740 vs. ExPASy Swiss-Prot
Match: O80341 (Ethylene-responsive transcription factor 5 OS=Arabidopsis thaliana OX=3702 GN=ERF5 PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 3.0e-49
Identity = 147/334 (44.01%), Postives = 191/334 (57.19%), Query Frame = 0

Query: 8   EAAALENIKLHLLGDLSPLPRILASDNLCVSSSDRSVSSDSSSTSNCVLNIFDYFDSDEI 67
           E +AL  I+ HLL + SP    +A+D      S  S +++SSS S+ ++     F S   
Sbjct: 6   EVSALWFIEKHLLDEASP----VATDPWMKHES--SSATESSSDSSSII-----FGS--- 65

Query: 68  FEFSDLSSSMSSNDDFFGFEMKPNVIDLTTPKSTELL----EFETKPTVFEDLSSRTLYS 127
                 SSS  +  DF     KP +IDL TP+S E L    EF+++ +V +     +  +
Sbjct: 66  ------SSSSFAPIDFSESVCKPEIIDLDTPRSMEFLSIPFEFDSEVSVSDFDFKPSNQN 125

Query: 128 CNSFEVESKISQVTNSNRKRANLKISLPNKSTQWINFDSAAEKKTPVVVQRSKDLEAEKK 187
            N FE E K      S  ++  LKISLP K T+WI F  AAE   P V   +K +  E+K
Sbjct: 126 QNQFEPELK------SQIRKPPLKISLPAK-TEWIQF--AAENTKPEV---TKPVSEEEK 185

Query: 188 VHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFKLRGSKAILNFP 247
            HYRGVRQRPWGKFAAEIRDPN+RGSRVWLGTF+TAIEAARAYD AAF+LRGSKAILNFP
Sbjct: 186 KHYRGVRQRPWGKFAAEIRDPNKRGSRVWLGTFDTAIEAARAYDEAAFRLRGSKAILNFP 245

Query: 248 LEAANSYSEPVAAGKRRREEEVEEVVEAVAVKKERTAEEVNPAAADVGYLKDMPLTPSSW 307
           LE            K+R+ ++ E+V     V K   + +VN         +  P   S+ 
Sbjct: 246 LEVGKWKPRADEGEKKRKRDDDEKVTVVEKVLKTEQSVDVNGG-------ETFPFVTSNL 300

Query: 308 SMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMVV 338
           + + D ++ G  N P LSPLSPHP FG+ QL VV
Sbjct: 306 TELCDWDLTGFLNFPLLSPLSPHPPFGYSQLTVV 300

BLAST of Lsi07G012740 vs. ExPASy Swiss-Prot
Match: Q40478 (Ethylene-responsive transcription factor 5 OS=Nicotiana tabacum OX=4097 GN=ERF5 PE=2 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 7.3e-48
Identity = 148/345 (42.90%), Postives = 195/345 (56.52%), Query Frame = 0

Query: 7   EEAAALENIKLHLLGD------LSPLPRILASDNLCVSSSDRSVSSDSSSTSNCVLNIFD 66
           E    L+ I+ HLL D        P P IL S +   SSS  S++S +S  +N      +
Sbjct: 6   ENCTTLDLIRQHLLDDNVFMEHYCPQP-ILYSQS---SSSSESLNSIASELNN------E 65

Query: 67  YFDSDEIFEFSDLSSSMSSNDDFFGFEMKPNVIDLTTPKSTELLEFETKPTVFEDLSSRT 126
            F  +   +++D  ++ SSN D   F          +    +  EFETKP V        
Sbjct: 66  TFSFEPTLKYAD--TAQSSNLDISSFFN-------NSKTEFDSFEFETKPNV-------- 125

Query: 127 LYSCNSFEVESKISQVTNSNRKRANLKISLPNKSTQWINFDSAAEKKTPVVVQRSKDLEA 186
               ++  + S   + T+   ++ +L I++P K  +              VVQ+ + +  
Sbjct: 126 ----SAARISSNSPKQTSFKERKPSLNIAIPMKQQE--------------VVQKVEVVPT 185

Query: 187 EKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFKLRGSKAIL 246
           EKK HYRGVRQRPWGKFAAEIRDPNR+G+RVWLGTF+TAIEAA+AYDRAAFKLRGSKAI+
Sbjct: 186 EKK-HYRGVRQRPWGKFAAEIRDPNRKGTRVWLGTFDTAIEAAKAYDRAAFKLRGSKAIV 245

Query: 247 NFPLEAA------NSYSEPVAAG-KRRREEEVEEVVEAVAVKKERTAEEVNPAAADVGYL 306
           NFPLE A      N   +P  +G KR RE E EE+V    +KKE   EE  PAAA     
Sbjct: 246 NFPLEVANFKQQDNEILQPANSGRKRMRETENEEIV----IKKEVKREERVPAAA----- 291

Query: 307 KDMPLTPSSWSMVWDGE-MKGLFNIPPLSPLSPHPAFGFPQLMVV 338
              PLTPSSWS +W+GE  KG+F +PPLSPLSPH A+   QL+++
Sbjct: 306 --APLTPSSWSAIWEGEDGKGIFEVPPLSPLSPHMAYS--QLVMI 291

BLAST of Lsi07G012740 vs. ExPASy Swiss-Prot
Match: Q8VZ91 (Ethylene-responsive transcription factor 6 OS=Arabidopsis thaliana OX=3702 GN=ERF6 PE=1 SV=2)

HSP 1 Score: 189.9 bits (481), Expect = 4.7e-47
Identity = 142/335 (42.39%), Postives = 186/335 (55.52%), Query Frame = 0

Query: 8   EAAALENIKLHLLGDLSPLPRILASDNLCVSSSDRSVSSDSSSTSNCVLNIFDYFDSDEI 67
           E +AL  IK +LL +LSPLP                    +++T+N  +N F  FD    
Sbjct: 6   EVSALFLIKKYLLDELSPLP--------------------TTATTNRWMNDFTSFDQTG- 65

Query: 68  FEFSDLSSSMSSNDDFFGFEMKPNVIDLTTPKSTELLEFETKPTVFEDLSSRTLYSCNSF 127
           FEFS+             FE KP +IDL TPK  E+ +F+ K  +  +       S +SF
Sbjct: 66  FEFSE-------------FETKPEIIDLVTPK-PEIFDFDVKSEIPSE-------SNDSF 125

Query: 128 EVESKISQVTNSNRKRANLKISLPNKSTQWINFDSAAEK-KTPVVVQRSKDLEAEKKVHY 187
             +S   +VT  + ++  LKI+ PN+ T+WI F +   K + PV V     + AE+K HY
Sbjct: 126 TFQSNPPRVTVQSNRKPPLKIAPPNR-TKWIQFATGNPKPELPVPV-----VAAEEKRHY 185

Query: 188 RGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFKLRGSKAILNFPLEA 247
           RGVR RPWGKFAAEIRDP RRG+RVWLGTFETAIEAARAYD+ AF+LRGSKAILNFPLE 
Sbjct: 186 RGVRMRPWGKFAAEIRDPTRRGTRVWLGTFETAIEAARAYDKEAFRLRGSKAILNFPLEV 245

Query: 248 A--NSYSEPVAA--GKRRREEEVEEVVEAVAVKKERTAEEVNPAAADVGYLKDMPLTPSS 307
              N  +E       KR+R+ E EEV     V K   + +V+                S 
Sbjct: 246 DKWNPRAEDGRGLYNKRKRDGEEEEVTVVEKVLKTEESYDVSGGEN----------VESG 282

Query: 308 WSMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMVV 338
            + + D ++    ++P LSPLSPHP FG+PQL VV
Sbjct: 306 LTAIDDWDLTEFLSMPLLSPLSPHPPFGYPQLTVV 282

BLAST of Lsi07G012740 vs. ExPASy Swiss-Prot
Match: Q9LW48 (Ethylene-responsive transcription factor 5 OS=Nicotiana sylvestris OX=4096 GN=ERF5 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.7e-41
Identity = 117/267 (43.82%), Postives = 151/267 (56.55%), Query Frame = 0

Query: 70  FSDLSSSMSSNDDFFGFEMKPNVIDLTTPKSTEL---LEFETKPTVFEDLSSRTLYSCNS 129
           +S  SSS  S D  F FE  P +   TT +S+ L     F    T F+     T+ + ++
Sbjct: 39  YSQSSSSSESLDQSFSFE--PTLNYATTAQSSNLEVSTFFNNSKTEFDSFEFGTIPNVSA 98

Query: 130 FEVESKISQVTNSNRKRANLKISLPNKSTQWINFDSAAEKKTPVVVQRSKDLEAEKKVHY 189
               S   + T+   ++ +L I++P K                 VVQ+ +    EKK HY
Sbjct: 99  --ARSSSLKQTSFKERKPSLNIAIPVKQE---------------VVQKVELAPTEKK-HY 158

Query: 190 RGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFKLRGSKAILNFPLEA 249
           RGVRQRPWGKFAAEIRDPNR+G+RVWLGTF+TAIEAA+AYDRAA+KLRGSKAI+NFPLE 
Sbjct: 159 RGVRQRPWGKFAAEIRDPNRKGTRVWLGTFDTAIEAAKAYDRAAYKLRGSKAIVNFPLEV 218

Query: 250 ANSYSE------PVAAGKRRREEEVEEVVEAVAVKKERTAEEVNPAAADVGYLKDMPLTP 309
           AN   E      P+    R+R  E     + V  K+ +  EE  P A         PLTP
Sbjct: 219 ANFKQEFNNEIRPLVNSSRKRVRETVNEEQLVINKEMKIEEERVPTA---------PLTP 276

Query: 310 SSWSMVWD-GEMKGLFNIPPLSPLSPH 327
           SSWS +WD G+ KG+F +PPLSP   +
Sbjct: 279 SSWSAIWDSGDGKGIFEVPPLSPFGAY 276

BLAST of Lsi07G012740 vs. ExPASy Swiss-Prot
Match: Q9FKG1 (Ethylene-responsive transcription factor ERF104 OS=Arabidopsis thaliana OX=3702 GN=ERF104 PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.9e-33
Identity = 78/158 (49.37%), Postives = 102/158 (64.56%), Query Frame = 0

Query: 175 SKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFKLR 234
           +K  + E++ HYRGVR+RPWGK+AAEIRDPN++G R+WLGT++TA+EA RAYD+AAF+LR
Sbjct: 77  TKTEKEEEERHYRGVRRRPWGKYAAEIRDPNKKGCRIWLGTYDTAVEAGRAYDQAAFQLR 136

Query: 235 GSKAILNFPLEA-----ANSYSEPVAAGKRRREEEVEEVVEAVAVKKERTAEEVNPAAAD 294
           G KAILNFPL+        S    +  GKR+R++      E  A  K    E       +
Sbjct: 137 GRKAILNFPLDVRVTSETCSGEGVIGLGKRKRDKGSPPEEEKAARVKVEEEESNTSETTE 196

Query: 295 VGYLKDMPLTPSSWSMVWD-GEMKGLFNIPPLSPLSPH 327
                 +PLTPSSW   WD G   G+F+IPPLSP SP+
Sbjct: 197 AEVEPVVPLTPSSWMGFWDVGAGDGIFSIPPLSPTSPN 234

BLAST of Lsi07G012740 vs. ExPASy TrEMBL
Match: A0A5A7TC77 (Ethylene-responsive transcription factor 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00570 PE=4 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.9e-148
Identity = 297/350 (84.86%), Postives = 310/350 (88.57%), Query Frame = 0

Query: 1   MEFVNLEEAAALENIKLHLLGDLSPLPRILAS--DNLCVSSSD-RSVSSDSSSTS-NCVL 60
           MEFVNLEEA ALE+IKLHLLG+LSP PRIL S  DNLCVSSSD RSVSS SSSTS NCVL
Sbjct: 1   MEFVNLEEAVALESIKLHLLGELSPFPRILPSDDDNLCVSSSDRRSVSSGSSSTSNNCVL 60

Query: 61  NIFDYFDSDEIFEFS-DL----SSSMSSNDDFFGFEMKPNVIDLTTPKSTELLEFETKPT 120
           NI DYF+SDEIFEFS DL    SSSMSSNDDFFGFEMKPNVIDLTTPKSTEL+EFETKP 
Sbjct: 61  NISDYFNSDEIFEFSPDLMPTESSSMSSNDDFFGFEMKPNVIDLTTPKSTELVEFETKPR 120

Query: 121 VFEDLSSRTLYSCNSFEVESKISQVTNSNRKRANLKISLPNKSTQWINFDSAAEKKTPVV 180
           VFED +SRT YS +S EVESKISQV NSNRKRANLKISLPNK+TQWINFDSA EKK PVV
Sbjct: 121 VFEDFNSRTQYSNSSIEVESKISQVANSNRKRANLKISLPNKTTQWINFDSAVEKKNPVV 180

Query: 181 V-QRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA 240
           V QRS+D+EAE+KVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA
Sbjct: 181 VQQRSRDVEAERKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA 240

Query: 241 FKLRGSKAILNFPLEAANSYSEPVAAGKRRREEEVEEVVEAVAVKKERTA---EEVNPAA 300
           FKLRGSKAILNFPLEA+NSYSEPV  GKRRRE   EE VEAV VKKE+     EEV  AA
Sbjct: 241 FKLRGSKAILNFPLEASNSYSEPVVVGKRRRE---EEEVEAVVVKKEKRGEEEEEVKQAA 300

Query: 301 ADVGYLKDMPLTPSSWSMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMVV 338
            DV YLKDMPLTPSSWSMVWDGE KG+FNIPPLSPLSPHPAFGFPQLMVV
Sbjct: 301 GDVSYLKDMPLTPSSWSMVWDGETKGVFNIPPLSPLSPHPAFGFPQLMVV 347

BLAST of Lsi07G012740 vs. ExPASy TrEMBL
Match: A0A1S3BL69 (ethylene-responsive transcription factor 5 OS=Cucumis melo OX=3656 GN=LOC103490863 PE=4 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.9e-148
Identity = 297/350 (84.86%), Postives = 310/350 (88.57%), Query Frame = 0

Query: 1   MEFVNLEEAAALENIKLHLLGDLSPLPRILAS--DNLCVSSSD-RSVSSDSSSTS-NCVL 60
           MEFVNLEEA ALE+IKLHLLG+LSP PRIL S  DNLCVSSSD RSVSS SSSTS NCVL
Sbjct: 1   MEFVNLEEAVALESIKLHLLGELSPFPRILPSDDDNLCVSSSDRRSVSSGSSSTSNNCVL 60

Query: 61  NIFDYFDSDEIFEFS-DL----SSSMSSNDDFFGFEMKPNVIDLTTPKSTELLEFETKPT 120
           NI DYF+SDEIFEFS DL    SSSMSSNDDFFGFEMKPNVIDLTTPKSTEL+EFETKP 
Sbjct: 61  NISDYFNSDEIFEFSPDLMPTESSSMSSNDDFFGFEMKPNVIDLTTPKSTELVEFETKPR 120

Query: 121 VFEDLSSRTLYSCNSFEVESKISQVTNSNRKRANLKISLPNKSTQWINFDSAAEKKTPVV 180
           VFED +SRT YS +S EVESKISQV NSNRKRANLKISLPNK+TQWINFDSA EKK PVV
Sbjct: 121 VFEDFNSRTQYSNSSIEVESKISQVANSNRKRANLKISLPNKTTQWINFDSAVEKKNPVV 180

Query: 181 V-QRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA 240
           V QRS+D+EAE+KVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA
Sbjct: 181 VQQRSRDVEAERKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA 240

Query: 241 FKLRGSKAILNFPLEAANSYSEPVAAGKRRREEEVEEVVEAVAVKKERTA---EEVNPAA 300
           FKLRGSKAILNFPLEA+NSYSEPV  GKRRRE   EE VEAV VKKE+     EEV  AA
Sbjct: 241 FKLRGSKAILNFPLEASNSYSEPVVVGKRRRE---EEEVEAVVVKKEKRGEEEEEVKQAA 300

Query: 301 ADVGYLKDMPLTPSSWSMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMVV 338
            DV YLKDMPLTPSSWSMVWDGE KG+FNIPPLSPLSPHPAFGFPQLMVV
Sbjct: 301 GDVSYLKDMPLTPSSWSMVWDGETKGVFNIPPLSPLSPHPAFGFPQLMVV 347

BLAST of Lsi07G012740 vs. ExPASy TrEMBL
Match: A0A0A0L465 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G018320 PE=4 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 4.2e-147
Identity = 291/346 (84.10%), Postives = 306/346 (88.44%), Query Frame = 0

Query: 1   MEFVNLEEAAALENIKLHLLGDLSPLPRILAS--DNLCVSSSDRSVSSDSSSTSNCVLNI 60
           MEFV+LEEA ALENIKLHLLG+LSP PRIL S  DNLCVSSSDRSVSS SSSTSNCVLNI
Sbjct: 1   MEFVDLEEAVALENIKLHLLGELSPFPRILPSNDDNLCVSSSDRSVSSGSSSTSNCVLNI 60

Query: 61  FDYFDSDEIFEF-SDL----SSSMSSNDDFFGFEMKPNVIDLTTPKSTELLEFETKPTVF 120
            D+F+SDEIF+F SDL    SSS+SSNDDFFGFEMKPNVIDLTTPKSTEL+EFE KP VF
Sbjct: 61  SDFFNSDEIFQFSSDLMPTQSSSVSSNDDFFGFEMKPNVIDLTTPKSTELVEFEMKPRVF 120

Query: 121 EDLSSRTLYSCNSFEVESKISQVTNSNRKRANLKISLPNKSTQWINFDSAAEKKTPVVV- 180
           ED +SRT YS +S EVESKISQVTNSNRKRANLKISLPNK+TQWINFDSA EKK  VVV 
Sbjct: 121 EDFNSRTRYSNSSIEVESKISQVTNSNRKRANLKISLPNKTTQWINFDSAVEKKNRVVVE 180

Query: 181 QRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFK 240
           QRS+D+EAE+KVHYRGVRQRPWGKFAAEIRDP RRGSRVWLGTFETAIEAARAYDRAAFK
Sbjct: 181 QRSRDVEAERKVHYRGVRQRPWGKFAAEIRDPTRRGSRVWLGTFETAIEAARAYDRAAFK 240

Query: 241 LRGSKAILNFPLEAANSYSEPVAAGKRRREEEVEEVVEAVAVKKE-RTAEEVNPAAADVG 300
           LRGSKAILNFPLEA+NSYSE V  GKRRREE  EE VEAV VKKE R  EEV     DV 
Sbjct: 241 LRGSKAILNFPLEASNSYSEAVVVGKRRREE--EEEVEAVVVKKEKREEEEVKQVGTDVS 300

Query: 301 YLKDMPLTPSSWSMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMVV 338
           YLKDMPLTPSSWSMVWDGE KG+FNIPPLSPLSPHPAFGFPQLMVV
Sbjct: 301 YLKDMPLTPSSWSMVWDGETKGVFNIPPLSPLSPHPAFGFPQLMVV 344

BLAST of Lsi07G012740 vs. ExPASy TrEMBL
Match: A0A6J1E649 (ethylene-responsive transcription factor 5-like OS=Cucurbita moschata OX=3662 GN=LOC111431140 PE=4 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 5.3e-142
Identity = 289/348 (83.05%), Postives = 305/348 (87.64%), Query Frame = 0

Query: 1   MEFVNLEEAAALENIKLHLLGDLSPLPRILAS-DNLCVSSSDRSVSSDSSSTSNCVLNIF 60
           ME VNLEEAAALENIKLHLLG+LSPLPR L S D+LCV+SSDRSVSSD+S+TSNC L I 
Sbjct: 1   MELVNLEEAAALENIKLHLLGELSPLPRNLTSDDSLCVTSSDRSVSSDASTTSNCPLTIT 60

Query: 61  DYFDSDEIFEFSD----LSSSMSSNDDFFGFEMKPNVIDLTTPKSTELLEFETKPTVFED 120
           DYFDSD+ FEFS       SS+ SNDDFFG EMKPNVIDLTTPKS E LEFETKP VFED
Sbjct: 61  DYFDSDDFFEFSSDFIREESSILSNDDFFGVEMKPNVIDLTTPKSEEFLEFETKPNVFED 120

Query: 121 --LSSRTLYSCNSFEVESK---ISQVTNSNRKRANLKISLPNKSTQWINFDSAAEKKTPV 180
             LSS +LYS +S EVESK   I +VTNSNRKR+NLKISLPNK+TQWINFD AAEKK PV
Sbjct: 121 LSLSSSSLYSNSSSEVESKTKLIGRVTNSNRKRSNLKISLPNKTTQWINFD-AAEKKNPV 180

Query: 181 VVQRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA 240
           VVQRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA
Sbjct: 181 VVQRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA 240

Query: 241 FKLRGSKAILNFPLEAANS-YSEPVAAGKRRREEEVEEVVEAVAVKKERTAEEVNPAAAD 300
           FKLRGSKAILNFPLEAANS YSEP+ AGKRRREEEVEEVVEAV VKKE+T EE+N  AAD
Sbjct: 241 FKLRGSKAILNFPLEAANSYYSEPIVAGKRRREEEVEEVVEAVPVKKEKT-EEMNLPAAD 300

Query: 301 VGYLKDMPLTPSSWSMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMVV 338
           V +LKDMPLTPSSWSMVWDGE KGL NIPPLSPLSP   FGFPQLMVV
Sbjct: 301 VSFLKDMPLTPSSWSMVWDGESKGLLNIPPLSPLSP---FGFPQLMVV 343

BLAST of Lsi07G012740 vs. ExPASy TrEMBL
Match: A0A6J1HMU9 (ethylene-responsive transcription factor 5 OS=Cucurbita maxima OX=3661 GN=LOC111465058 PE=4 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 2.6e-141
Identity = 289/348 (83.05%), Postives = 307/348 (88.22%), Query Frame = 0

Query: 1   MEFVNLEEAAALENIKLHLLGDLSPLPRILAS-DNLCVSSSDRSVSSDSSSTSNCVLNIF 60
           ME VNLEEAAALENIKLHLLG+LSPLPR LAS D+LCV+SSDRSVSSD+S+TSNC L I 
Sbjct: 1   MELVNLEEAAALENIKLHLLGELSPLPRNLASDDSLCVTSSDRSVSSDASTTSNCPLTIT 60

Query: 61  DYFDSDEIFEF-SDL---SSSMSSNDDFFGFEMKPNVIDLTTPKSTELLEFETKPTVFED 120
           DYFD D+ FEF SDL    SS+ SNDDFFG EMKPNVIDLT PKS E LEFETKP+VFED
Sbjct: 61  DYFDPDDFFEFSSDLIQDESSILSNDDFFGVEMKPNVIDLTMPKSEEFLEFETKPSVFED 120

Query: 121 --LSSRTLYSCNSFEVESK---ISQVTNSNRKRANLKISLPNKSTQWINFDSAAEKKTPV 180
             LSS ++YS +SFEVESK   I +VTNSNRK +NLKISLPNK+TQWINFD AAEKK PV
Sbjct: 121 LSLSSSSMYSNSSFEVESKTKLIGRVTNSNRKPSNLKISLPNKTTQWINFD-AAEKKNPV 180

Query: 181 VVQRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA 240
           VVQRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA
Sbjct: 181 VVQRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA 240

Query: 241 FKLRGSKAILNFPLEAANS-YSEPVAAGKRRREEEVEEVVEAVAVKKERTAEEVNPAAAD 300
           FKLRGSKAILNFPLEAANS YSEP+ AGKRRREEEVEEVVEAV VKKE+T EE+N  AAD
Sbjct: 241 FKLRGSKAILNFPLEAANSYYSEPIVAGKRRREEEVEEVVEAVPVKKEKT-EEMNFPAAD 300

Query: 301 VGYLKDMPLTPSSWSMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMVV 338
           V +LKDMPLTPSSWSMVWDGE KGL NIPPLSPLSP   FGFPQLMVV
Sbjct: 301 VSFLKDMPLTPSSWSMVWDGESKGLLNIPPLSPLSP---FGFPQLMVV 343

BLAST of Lsi07G012740 vs. NCBI nr
Match: XP_038903436.1 (ethylene-responsive transcription factor 5-like [Benincasa hispida])

HSP 1 Score: 550.8 bits (1418), Expect = 8.0e-153
Identity = 299/345 (86.67%), Postives = 314/345 (91.01%), Query Frame = 0

Query: 1   MEFVNLEEAAALENIKLHLLGDLSPLPRILASD-NLCVSSSDRSVSSDSSSTSNCVLNIF 60
           MEFVNLEEAAALENIKLHLLG+LSPLPRIL  D NLCVSSSDRSVSSDSSSTSNC+LNI 
Sbjct: 1   MEFVNLEEAAALENIKLHLLGELSPLPRILNYDNNLCVSSSDRSVSSDSSSTSNCLLNIS 60

Query: 61  DYFDSDEIFEFSD----LSSSMSSNDDFFGFEMKPNVIDLTTPKSTELLEFETKPTVFED 120
           DYF+SDEIFEFS       SSMSSN+DFFGFEMKPNVIDLTTPKSTELLEFETKP +FED
Sbjct: 61  DYFNSDEIFEFSSDLMRTESSMSSNEDFFGFEMKPNVIDLTTPKSTELLEFETKPRIFED 120

Query: 121 LSSRTLYSCNSFEVESKISQVTNSNRKRANLKISLPNKSTQWINFDSAAEKKTPVVVQRS 180
            SSRT    +SFEVESKISQV NSNRKRANLKISLPNK+TQWINFD+ AEKK PVVVQ+S
Sbjct: 121 FSSRT----SSFEVESKISQVGNSNRKRANLKISLPNKTTQWINFDT-AEKKNPVVVQKS 180

Query: 181 KDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFKLRG 240
           +D+EAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFKLRG
Sbjct: 181 RDVEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFKLRG 240

Query: 241 SKAILNFPLEAANSYSEPVAAGKRRREEEVEEVVEAVAVKKERTAEEVN---PAAADVGY 300
           SKAILNFPLEAA+SY EPVA GKRRRE   EEVVEAV  KKE+TAEEVN   PA+ADVGY
Sbjct: 241 SKAILNFPLEAASSYLEPVAVGKRRRE---EEVVEAVVAKKEKTAEEVNVNPPASADVGY 300

Query: 301 LKDMPLTPSSWSMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMVV 338
           LKDMPLTPSSWSMVWDGE KG+FNIPPLSPLSPHPAFGFPQLMVV
Sbjct: 301 LKDMPLTPSSWSMVWDGETKGVFNIPPLSPLSPHPAFGFPQLMVV 337

BLAST of Lsi07G012740 vs. NCBI nr
Match: XP_008448805.1 (PREDICTED: ethylene-responsive transcription factor 5 [Cucumis melo] >KAA0041064.1 ethylene-responsive transcription factor 5 [Cucumis melo var. makuwa] >TYK12044.1 ethylene-responsive transcription factor 5 [Cucumis melo var. makuwa])

HSP 1 Score: 534.6 bits (1376), Expect = 6.0e-148
Identity = 297/350 (84.86%), Postives = 310/350 (88.57%), Query Frame = 0

Query: 1   MEFVNLEEAAALENIKLHLLGDLSPLPRILAS--DNLCVSSSD-RSVSSDSSSTS-NCVL 60
           MEFVNLEEA ALE+IKLHLLG+LSP PRIL S  DNLCVSSSD RSVSS SSSTS NCVL
Sbjct: 1   MEFVNLEEAVALESIKLHLLGELSPFPRILPSDDDNLCVSSSDRRSVSSGSSSTSNNCVL 60

Query: 61  NIFDYFDSDEIFEFS-DL----SSSMSSNDDFFGFEMKPNVIDLTTPKSTELLEFETKPT 120
           NI DYF+SDEIFEFS DL    SSSMSSNDDFFGFEMKPNVIDLTTPKSTEL+EFETKP 
Sbjct: 61  NISDYFNSDEIFEFSPDLMPTESSSMSSNDDFFGFEMKPNVIDLTTPKSTELVEFETKPR 120

Query: 121 VFEDLSSRTLYSCNSFEVESKISQVTNSNRKRANLKISLPNKSTQWINFDSAAEKKTPVV 180
           VFED +SRT YS +S EVESKISQV NSNRKRANLKISLPNK+TQWINFDSA EKK PVV
Sbjct: 121 VFEDFNSRTQYSNSSIEVESKISQVANSNRKRANLKISLPNKTTQWINFDSAVEKKNPVV 180

Query: 181 V-QRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA 240
           V QRS+D+EAE+KVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA
Sbjct: 181 VQQRSRDVEAERKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA 240

Query: 241 FKLRGSKAILNFPLEAANSYSEPVAAGKRRREEEVEEVVEAVAVKKERTA---EEVNPAA 300
           FKLRGSKAILNFPLEA+NSYSEPV  GKRRRE   EE VEAV VKKE+     EEV  AA
Sbjct: 241 FKLRGSKAILNFPLEASNSYSEPVVVGKRRRE---EEEVEAVVVKKEKRGEEEEEVKQAA 300

Query: 301 ADVGYLKDMPLTPSSWSMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMVV 338
            DV YLKDMPLTPSSWSMVWDGE KG+FNIPPLSPLSPHPAFGFPQLMVV
Sbjct: 301 GDVSYLKDMPLTPSSWSMVWDGETKGVFNIPPLSPLSPHPAFGFPQLMVV 347

BLAST of Lsi07G012740 vs. NCBI nr
Match: XP_004147224.1 (ethylene-responsive transcription factor 6 [Cucumis sativus] >KGN55839.1 hypothetical protein Csa_010328 [Cucumis sativus])

HSP 1 Score: 530.8 bits (1366), Expect = 8.6e-147
Identity = 291/346 (84.10%), Postives = 306/346 (88.44%), Query Frame = 0

Query: 1   MEFVNLEEAAALENIKLHLLGDLSPLPRILAS--DNLCVSSSDRSVSSDSSSTSNCVLNI 60
           MEFV+LEEA ALENIKLHLLG+LSP PRIL S  DNLCVSSSDRSVSS SSSTSNCVLNI
Sbjct: 1   MEFVDLEEAVALENIKLHLLGELSPFPRILPSNDDNLCVSSSDRSVSSGSSSTSNCVLNI 60

Query: 61  FDYFDSDEIFEF-SDL----SSSMSSNDDFFGFEMKPNVIDLTTPKSTELLEFETKPTVF 120
            D+F+SDEIF+F SDL    SSS+SSNDDFFGFEMKPNVIDLTTPKSTEL+EFE KP VF
Sbjct: 61  SDFFNSDEIFQFSSDLMPTQSSSVSSNDDFFGFEMKPNVIDLTTPKSTELVEFEMKPRVF 120

Query: 121 EDLSSRTLYSCNSFEVESKISQVTNSNRKRANLKISLPNKSTQWINFDSAAEKKTPVVV- 180
           ED +SRT YS +S EVESKISQVTNSNRKRANLKISLPNK+TQWINFDSA EKK  VVV 
Sbjct: 121 EDFNSRTRYSNSSIEVESKISQVTNSNRKRANLKISLPNKTTQWINFDSAVEKKNRVVVE 180

Query: 181 QRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFK 240
           QRS+D+EAE+KVHYRGVRQRPWGKFAAEIRDP RRGSRVWLGTFETAIEAARAYDRAAFK
Sbjct: 181 QRSRDVEAERKVHYRGVRQRPWGKFAAEIRDPTRRGSRVWLGTFETAIEAARAYDRAAFK 240

Query: 241 LRGSKAILNFPLEAANSYSEPVAAGKRRREEEVEEVVEAVAVKKE-RTAEEVNPAAADVG 300
           LRGSKAILNFPLEA+NSYSE V  GKRRREE  EE VEAV VKKE R  EEV     DV 
Sbjct: 241 LRGSKAILNFPLEASNSYSEAVVVGKRRREE--EEEVEAVVVKKEKREEEEVKQVGTDVS 300

Query: 301 YLKDMPLTPSSWSMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMVV 338
           YLKDMPLTPSSWSMVWDGE KG+FNIPPLSPLSPHPAFGFPQLMVV
Sbjct: 301 YLKDMPLTPSSWSMVWDGETKGVFNIPPLSPLSPHPAFGFPQLMVV 344

BLAST of Lsi07G012740 vs. NCBI nr
Match: XP_022923447.1 (ethylene-responsive transcription factor 5-like [Cucurbita moschata])

HSP 1 Score: 513.8 bits (1322), Expect = 1.1e-141
Identity = 289/348 (83.05%), Postives = 305/348 (87.64%), Query Frame = 0

Query: 1   MEFVNLEEAAALENIKLHLLGDLSPLPRILAS-DNLCVSSSDRSVSSDSSSTSNCVLNIF 60
           ME VNLEEAAALENIKLHLLG+LSPLPR L S D+LCV+SSDRSVSSD+S+TSNC L I 
Sbjct: 1   MELVNLEEAAALENIKLHLLGELSPLPRNLTSDDSLCVTSSDRSVSSDASTTSNCPLTIT 60

Query: 61  DYFDSDEIFEFSD----LSSSMSSNDDFFGFEMKPNVIDLTTPKSTELLEFETKPTVFED 120
           DYFDSD+ FEFS       SS+ SNDDFFG EMKPNVIDLTTPKS E LEFETKP VFED
Sbjct: 61  DYFDSDDFFEFSSDFIREESSILSNDDFFGVEMKPNVIDLTTPKSEEFLEFETKPNVFED 120

Query: 121 --LSSRTLYSCNSFEVESK---ISQVTNSNRKRANLKISLPNKSTQWINFDSAAEKKTPV 180
             LSS +LYS +S EVESK   I +VTNSNRKR+NLKISLPNK+TQWINFD AAEKK PV
Sbjct: 121 LSLSSSSLYSNSSSEVESKTKLIGRVTNSNRKRSNLKISLPNKTTQWINFD-AAEKKNPV 180

Query: 181 VVQRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA 240
           VVQRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA
Sbjct: 181 VVQRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA 240

Query: 241 FKLRGSKAILNFPLEAANS-YSEPVAAGKRRREEEVEEVVEAVAVKKERTAEEVNPAAAD 300
           FKLRGSKAILNFPLEAANS YSEP+ AGKRRREEEVEEVVEAV VKKE+T EE+N  AAD
Sbjct: 241 FKLRGSKAILNFPLEAANSYYSEPIVAGKRRREEEVEEVVEAVPVKKEKT-EEMNLPAAD 300

Query: 301 VGYLKDMPLTPSSWSMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMVV 338
           V +LKDMPLTPSSWSMVWDGE KGL NIPPLSPLSP   FGFPQLMVV
Sbjct: 301 VSFLKDMPLTPSSWSMVWDGESKGLLNIPPLSPLSP---FGFPQLMVV 343

BLAST of Lsi07G012740 vs. NCBI nr
Match: KAG6596875.1 (Ethylene-responsive transcription factor 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030150.1 Ethylene-responsive transcription factor 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 513.1 bits (1320), Expect = 1.9e-141
Identity = 287/354 (81.07%), Postives = 304/354 (85.88%), Query Frame = 0

Query: 1   MEFVNLEEAAALENIKLHLLGDLSPLPRILASDNLCVSSSDRSVSS--DSSSTSNCVLNI 60
           ME VNLE+AAAL+NIKLHLLG+LSPLP  LA DNLCVSSSDRS+SS   SSSTSNC L I
Sbjct: 1   METVNLEQAAALQNIKLHLLGELSPLPTNLAFDNLCVSSSDRSMSSGASSSSTSNCPLTI 60

Query: 61  FDYFDSDEIFEFSD----LSSSMSSNDDFFGFEMKPNVIDLTTPKSTELLEFETKPTVFE 120
            DYF+SDE FEFS       SSMSSNDDFFGFEMKPNVIDLTT  S E +EFETKP VF 
Sbjct: 61  SDYFNSDENFEFSSDFIRCESSMSSNDDFFGFEMKPNVIDLTTMNSAESIEFETKPFVFN 120

Query: 121 DLSSR------TLYSCNSFEVESK---ISQVTNSNRKRANLKISLPNKSTQWINFDSAAE 180
           D SS       +LYS +SFEVESK   ISQ  NSNRKRANLKISLPNK+TQWINFD+ AE
Sbjct: 121 DSSSSSSSSSISLYSKSSFEVESKTNLISQAANSNRKRANLKISLPNKTTQWINFDT-AE 180

Query: 181 KKTPVVVQRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARA 240
           KK PVVVQRSKDLEAE+KVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARA
Sbjct: 181 KKNPVVVQRSKDLEAERKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARA 240

Query: 241 YDRAAFKLRGSKAILNFPLEAANSYSEPVAAGKRRREEEVEEVVEAVAVKKERTAEEVNP 300
           YDRAAFKLRGSKAILNFPLEA NSYSEP+AAGKR+REEEV EVVEAVA+KKE+  EEVNP
Sbjct: 241 YDRAAFKLRGSKAILNFPLEATNSYSEPIAAGKRQREEEVGEVVEAVALKKEK-VEEVNP 300

Query: 301 A---AADVGYLKDMPLTPSSWSMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMV 337
           A   AADVGYLKDMPLTPS WSMVWDGE KG+FNIPPLSPLSPHPAFGF QL+V
Sbjct: 301 ASVPAADVGYLKDMPLTPSIWSMVWDGETKGVFNIPPLSPLSPHPAFGFSQLVV 352

BLAST of Lsi07G012740 vs. TAIR 10
Match: AT5G47230.1 (ethylene responsive element binding factor 5 )

HSP 1 Score: 197.2 bits (500), Expect = 2.1e-50
Identity = 147/334 (44.01%), Postives = 191/334 (57.19%), Query Frame = 0

Query: 8   EAAALENIKLHLLGDLSPLPRILASDNLCVSSSDRSVSSDSSSTSNCVLNIFDYFDSDEI 67
           E +AL  I+ HLL + SP    +A+D      S  S +++SSS S+ ++     F S   
Sbjct: 6   EVSALWFIEKHLLDEASP----VATDPWMKHES--SSATESSSDSSSII-----FGS--- 65

Query: 68  FEFSDLSSSMSSNDDFFGFEMKPNVIDLTTPKSTELL----EFETKPTVFEDLSSRTLYS 127
                 SSS  +  DF     KP +IDL TP+S E L    EF+++ +V +     +  +
Sbjct: 66  ------SSSSFAPIDFSESVCKPEIIDLDTPRSMEFLSIPFEFDSEVSVSDFDFKPSNQN 125

Query: 128 CNSFEVESKISQVTNSNRKRANLKISLPNKSTQWINFDSAAEKKTPVVVQRSKDLEAEKK 187
            N FE E K      S  ++  LKISLP K T+WI F  AAE   P V   +K +  E+K
Sbjct: 126 QNQFEPELK------SQIRKPPLKISLPAK-TEWIQF--AAENTKPEV---TKPVSEEEK 185

Query: 188 VHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFKLRGSKAILNFP 247
            HYRGVRQRPWGKFAAEIRDPN+RGSRVWLGTF+TAIEAARAYD AAF+LRGSKAILNFP
Sbjct: 186 KHYRGVRQRPWGKFAAEIRDPNKRGSRVWLGTFDTAIEAARAYDEAAFRLRGSKAILNFP 245

Query: 248 LEAANSYSEPVAAGKRRREEEVEEVVEAVAVKKERTAEEVNPAAADVGYLKDMPLTPSSW 307
           LE            K+R+ ++ E+V     V K   + +VN         +  P   S+ 
Sbjct: 246 LEVGKWKPRADEGEKKRKRDDDEKVTVVEKVLKTEQSVDVNGG-------ETFPFVTSNL 300

Query: 308 SMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMVV 338
           + + D ++ G  N P LSPLSPHP FG+ QL VV
Sbjct: 306 TELCDWDLTGFLNFPLLSPLSPHPPFGYSQLTVV 300

BLAST of Lsi07G012740 vs. TAIR 10
Match: AT4G17490.1 (ethylene responsive element binding factor 6 )

HSP 1 Score: 189.9 bits (481), Expect = 3.4e-48
Identity = 142/335 (42.39%), Postives = 186/335 (55.52%), Query Frame = 0

Query: 8   EAAALENIKLHLLGDLSPLPRILASDNLCVSSSDRSVSSDSSSTSNCVLNIFDYFDSDEI 67
           E +AL  IK +LL +LSPLP                    +++T+N  +N F  FD    
Sbjct: 6   EVSALFLIKKYLLDELSPLP--------------------TTATTNRWMNDFTSFDQTG- 65

Query: 68  FEFSDLSSSMSSNDDFFGFEMKPNVIDLTTPKSTELLEFETKPTVFEDLSSRTLYSCNSF 127
           FEFS+             FE KP +IDL TPK  E+ +F+ K  +  +       S +SF
Sbjct: 66  FEFSE-------------FETKPEIIDLVTPK-PEIFDFDVKSEIPSE-------SNDSF 125

Query: 128 EVESKISQVTNSNRKRANLKISLPNKSTQWINFDSAAEK-KTPVVVQRSKDLEAEKKVHY 187
             +S   +VT  + ++  LKI+ PN+ T+WI F +   K + PV V     + AE+K HY
Sbjct: 126 TFQSNPPRVTVQSNRKPPLKIAPPNR-TKWIQFATGNPKPELPVPV-----VAAEEKRHY 185

Query: 188 RGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFKLRGSKAILNFPLEA 247
           RGVR RPWGKFAAEIRDP RRG+RVWLGTFETAIEAARAYD+ AF+LRGSKAILNFPLE 
Sbjct: 186 RGVRMRPWGKFAAEIRDPTRRGTRVWLGTFETAIEAARAYDKEAFRLRGSKAILNFPLEV 245

Query: 248 A--NSYSEPVAA--GKRRREEEVEEVVEAVAVKKERTAEEVNPAAADVGYLKDMPLTPSS 307
              N  +E       KR+R+ E EEV     V K   + +V+                S 
Sbjct: 246 DKWNPRAEDGRGLYNKRKRDGEEEEVTVVEKVLKTEESYDVSGGEN----------VESG 282

Query: 308 WSMVWDGEMKGLFNIPPLSPLSPHPAFGFPQLMVV 338
            + + D ++    ++P LSPLSPHP FG+PQL VV
Sbjct: 306 LTAIDDWDLTEFLSMPLLSPLSPHPPFGYPQLTVV 282

BLAST of Lsi07G012740 vs. TAIR 10
Match: AT5G61600.1 (ethylene response factor 104 )

HSP 1 Score: 143.7 bits (361), Expect = 2.8e-34
Identity = 78/158 (49.37%), Postives = 102/158 (64.56%), Query Frame = 0

Query: 175 SKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFKLR 234
           +K  + E++ HYRGVR+RPWGK+AAEIRDPN++G R+WLGT++TA+EA RAYD+AAF+LR
Sbjct: 77  TKTEKEEEERHYRGVRRRPWGKYAAEIRDPNKKGCRIWLGTYDTAVEAGRAYDQAAFQLR 136

Query: 235 GSKAILNFPLEA-----ANSYSEPVAAGKRRREEEVEEVVEAVAVKKERTAEEVNPAAAD 294
           G KAILNFPL+        S    +  GKR+R++      E  A  K    E       +
Sbjct: 137 GRKAILNFPLDVRVTSETCSGEGVIGLGKRKRDKGSPPEEEKAARVKVEEEESNTSETTE 196

Query: 295 VGYLKDMPLTPSSWSMVWD-GEMKGLFNIPPLSPLSPH 327
                 +PLTPSSW   WD G   G+F+IPPLSP SP+
Sbjct: 197 AEVEPVVPLTPSSWMGFWDVGAGDGIFSIPPLSPTSPN 234

BLAST of Lsi07G012740 vs. TAIR 10
Match: AT5G51190.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 142.9 bits (359), Expect = 4.7e-34
Identity = 82/163 (50.31%), Postives = 107/163 (65.64%), Query Frame = 0

Query: 185 HYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFKLRGSKAILNFPL 244
           HYRGVR+RPWGK+AAEIRDPN++G RVWLGTF+TA+EAAR YD+AAFKLRGSKAILNFPL
Sbjct: 71  HYRGVRRRPWGKYAAEIRDPNKKGVRVWLGTFDTAMEAARGYDKAAFKLRGSKAILNFPL 130

Query: 245 EAA-------NSYSEPVAAGKRRR-EEEVEEVVEAVAVKKERTAEEVNPAAADVGYLKDM 304
           EA        N  +  + A ++R+  E+  +++   AVK+E    + +            
Sbjct: 131 EAGKHEDLGDNKKTISLKAKRKRQVTEDESQLISRKAVKREEAQVQADAC---------- 190

Query: 305 PLTPSSWSMVWDG---EMKGLFNIPPLSPLSPHPAFGFPQLMV 337
           PLTPSSW   WDG   +  G+F++P LSP    P+ G  QL+V
Sbjct: 191 PLTPSSWKGFWDGADSKDMGIFSVPLLSPC---PSLGHSQLVV 220

BLAST of Lsi07G012740 vs. TAIR 10
Match: AT5G61590.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 119.4 bits (298), Expect = 5.6e-27
Identity = 60/97 (61.86%), Postives = 74/97 (76.29%), Query Frame = 0

Query: 171 VVQRSKDLEAEKKVHYRGVRQRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAA 230
           V  R K    E+  HYRGVR+RPWGKFAAEIRDP ++GSR+WLGTFE+ I+AARAYD AA
Sbjct: 92  VSNRKKTKRFEETRHYRGVRRRPWGKFAAEIRDPAKKGSRIWLGTFESDIDAARAYDYAA 151

Query: 231 FKLRGSKAILNFPLEAANSYSEPVAAGKRRREEEVEE 268
           FKLRG KA+LNFPL+ A  Y  PV + ++RR  +V +
Sbjct: 152 FKLRGRKAVLNFPLD-AGKYDAPVNSCRKRRRTDVPQ 187

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O803413.0e-4944.01Ethylene-responsive transcription factor 5 OS=Arabidopsis thaliana OX=3702 GN=ER... [more]
Q404787.3e-4842.90Ethylene-responsive transcription factor 5 OS=Nicotiana tabacum OX=4097 GN=ERF5 ... [more]
Q8VZ914.7e-4742.39Ethylene-responsive transcription factor 6 OS=Arabidopsis thaliana OX=3702 GN=ER... [more]
Q9LW481.7e-4143.82Ethylene-responsive transcription factor 5 OS=Nicotiana sylvestris OX=4096 GN=ER... [more]
Q9FKG13.9e-3349.37Ethylene-responsive transcription factor ERF104 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A5A7TC772.9e-14884.86Ethylene-responsive transcription factor 5 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3BL692.9e-14884.86ethylene-responsive transcription factor 5 OS=Cucumis melo OX=3656 GN=LOC1034908... [more]
A0A0A0L4654.2e-14784.10AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G018320 PE=... [more]
A0A6J1E6495.3e-14283.05ethylene-responsive transcription factor 5-like OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1HMU92.6e-14183.05ethylene-responsive transcription factor 5 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_038903436.18.0e-15386.67ethylene-responsive transcription factor 5-like [Benincasa hispida][more]
XP_008448805.16.0e-14884.86PREDICTED: ethylene-responsive transcription factor 5 [Cucumis melo] >KAA0041064... [more]
XP_004147224.18.6e-14784.10ethylene-responsive transcription factor 6 [Cucumis sativus] >KGN55839.1 hypothe... [more]
XP_022923447.11.1e-14183.05ethylene-responsive transcription factor 5-like [Cucurbita moschata][more]
KAG6596875.11.9e-14181.07Ethylene-responsive transcription factor 5, partial [Cucurbita argyrosperma subs... [more]
Match NameE-valueIdentityDescription
AT5G47230.12.1e-5044.01ethylene responsive element binding factor 5 [more]
AT4G17490.13.4e-4842.39ethylene responsive element binding factor 6 [more]
AT5G61600.12.8e-3449.37ethylene response factor 104 [more]
AT5G51190.14.7e-3450.31Integrase-type DNA-binding superfamily protein [more]
AT5G61590.15.6e-2761.86Integrase-type DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 186..197
score: 74.24
coord: 225..245
score: 59.74
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 185..249
e-value: 1.4E-38
score: 144.2
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 185..235
e-value: 1.2E-15
score: 57.4
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 185..243
score: 24.447287
IPR001471AP2/ERF domainCDDcd00018AP2coord: 185..243
e-value: 8.08583E-32
score: 111.98
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 184..245
e-value: 2.9E-29
score: 103.0
NoneNo IPR availablePANTHERPTHR31677AP2 DOMAIN CLASS TRANSCRIPTION FACTORcoord: 7..100
NoneNo IPR availablePANTHERPTHR31677:SF64ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 6coord: 7..100
NoneNo IPR availablePANTHERPTHR31677AP2 DOMAIN CLASS TRANSCRIPTION FACTORcoord: 104..322
NoneNo IPR availablePANTHERPTHR31677:SF64ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 6coord: 104..322
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 185..244

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G012740.1Lsi07G012740.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity