Lsi07G012210 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi07G012210 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 6.9e-102
Identity = 197/440 (44.77%), Postives = 268/440 (60.91%), Query Frame = 0

Query: 21  LKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNVIHNLSIRYGKSD 80
           ++ LNGWYY+ K+ +    LPPG MGWPL+G  LS++K+FSSG P + I+NL ++YG+S 
Sbjct: 24  VRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKDFSSGHPDSFINNLVLKYGRSG 83

Query: 81  MYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLMQVFKGEHRRLRR 140
           +YK HL G  SIIVC  ++CR+VL+D+  FK   P+SI  L+  + ++ V   EHR  RR
Sbjct: 84  IYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMIDVSNAEHRLFRR 143

Query: 141 LTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTFKIIWNIFMGSSV 200
           L T+PI GH AL MY+  +E  VI+ LEE +SMK P+ELL E+KK++FK I ++FMGSS 
Sbjct: 144 LITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFKAIVHVFMGSSN 203

Query: 201 HSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKELLNKLQCIINEKRL-VKKREG 260
              I +I + F  +  G  S+PIN PGF+FHK+L+ARK+L   +Q +++E+RL ++    
Sbjct: 204 QDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARKKLAKIVQPVVDERRLMIENGPQ 263

Query: 261 ESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGHETSAYTAMWAVLFLTNHPHI 320
           E  + KD++D+L+E+KDE+G +L+DE I DL+ G LFAGHE++A + MW++ +LT HPHI
Sbjct: 264 EGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLFAGHESTATSLMWSITYLTQHPHI 323

Query: 321 FQKAK------------------------------VIDETLRVGGITFMVFREATIDVEI 380
            +KAK                              VIDETLR   I F  FREAT DV I
Sbjct: 324 LKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQVIDETLRCANIAFATFREATADVNI 383

Query: 381 ---------------------------------------NATPGAFTPFGLGSRYCPGSD 391
                                                  NA  G F PFG GSR CPG+D
Sbjct: 384 NGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWDDYNAKAGTFLPFGAGSRLCPGAD 443

BLAST of Lsi07G012210 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 3.7e-87
Identity = 186/464 (40.09%), Postives = 257/464 (55.39%), Query Frame = 0

Query: 20  VLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNVIHNLSIRYGKS 79
           +++++N W YE+ +G+    LPPG +GWP +G+ LS+L+ F +  P +    L  RYG  
Sbjct: 25  LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84

Query: 80  DMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLMQVFKGEHRRLR 139
            +YKAH+ G  SIIV TS+ CR+VL+D+D FKP  P S   L GRKS + +   EH+RLR
Sbjct: 85  GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144

Query: 140 RLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTFKIIWNIFMGSS 199
           RLT AP++GH AL  YI +IE  VI+ L++W  M    E LT ++KLTF+II  IF+ S 
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204

Query: 200 VHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKELLNKLQCIINEKRLVKKREG 259
             + +  +E  +  +  G  ++ +N PGF++H++LKARK L+   Q I+ E+R  +K+  
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNI 264

Query: 260 ESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGHETSAYTAMWAVLFLTNHPHI 319
            S   KDM+D L+ +KDEDG+ LDDE IID++   L AGHE+S +T MWA +FL  HP +
Sbjct: 265 LS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 324

Query: 320 FQKAK------------------------------VIDETLRVGGITFMVFREATIDVEI 379
            Q+AK                              V+DETLRV   +   FREA  DVE+
Sbjct: 325 LQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVEM 384

Query: 380 NA----------------------------------------TPGAFTPFGLGSRYCPGS 413
           N                                           GAF PFG GS  CPG+
Sbjct: 385 NGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLCPGN 444

BLAST of Lsi07G012210 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 317.4 bits (812), Expect = 2.6e-85
Identity = 186/471 (39.49%), Postives = 261/471 (55.41%), Query Frame = 0

Query: 9   LIFAALVLVYGVLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNV 68
           LI   L ++  VLKR+N W Y +K+G+    LPPG +GWP++G+  S+L+ F +  P + 
Sbjct: 13  LIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESF 72

Query: 69  IHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLM 128
           I +   RYG++ +YKAH+ G   ++V T E CR+VL+D+D F    P+S   L GRKS +
Sbjct: 73  IQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFV 132

Query: 129 QVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTF 188
            +   EH+RLRRLT+AP++G  AL +YI  IE TV + LE+W+ M   IE L+ ++KLTF
Sbjct: 133 GISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTF 192

Query: 189 KIIWNIFMGSSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKELLNKLQCII 248
           K+I  IF+ S     +  +E  +  +  G  ++ IN PGF++H++LKARK+L+   Q I+
Sbjct: 193 KVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIV 252

Query: 249 NEKRLVKKREGESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGHETSAYTAMW 308
             +R  +K+   S   KDM+D LI++KDE+G  LDDE IIDL+   L AGHE+S +  MW
Sbjct: 253 TNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMW 312

Query: 309 AVLFLTNHPHIFQKAK-----------------------------VIDETLRVGGITFMV 368
           A + +  HP I QKAK                             VIDETLRV   +   
Sbjct: 313 ATILMQEHPMILQKAKEEQERIVKKRAPGQKLTLKETREMVYLSQVIDETLRVITFSLTA 372

Query: 369 FREATIDVEINA-------------------------------------TP--GAFTPFG 412
           FREA  DV+++                                      TP  G F PFG
Sbjct: 373 FREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFG 432

BLAST of Lsi07G012210 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 9.1e-78
Identity = 167/446 (37.44%), Postives = 247/446 (55.38%), Query Frame = 0

Query: 13  ALVLVYGVLKRLNGWYYEAKVGKL-WPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNVIHN 72
           ALV+V    +R +GWY EA +G     +LPPG MGWPL+G   ++L+ F SG+P   I +
Sbjct: 44  ALVVVDAAARRAHGWYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFKSGKPDAFIAS 103

Query: 73  LSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLMQVF 132
              R+G++ +Y++ +    +++V T+E C+QVL D+D F    P++   L G +S + + 
Sbjct: 104 FVRRFGRTGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMP 163

Query: 133 KGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTFKII 192
             EHRR+R+LT API+G  AL  Y+  I+ TV S L  WA     +E LTE++++TFKII
Sbjct: 164 YDEHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKII 223

Query: 193 WNIFMGSSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKELLNKLQCIINEK 252
             IF+G +  ++   +E  + ++  G  ++ IN PGF++  +L+AR+ L+  LQ +++E+
Sbjct: 224 VQIFLGGADQATTRALERSYTELNYGMRAMAINLPGFAYRGALRARRRLVAVLQGVLDER 283

Query: 253 RLVKKREGESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGHETSAYTAMWAVL 312
           R  + + G S    DMMD LIE +DE G  LDD+ IID++   L AGHE+S +  MWA +
Sbjct: 284 RAARAK-GVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGHESSGHITMWATV 343

Query: 313 FLTNHPHIFQKAK------------------------------VIDETLRVGGITFMVFR 372
           FL  +P +F +AK                              VIDETLR+  I+F+ FR
Sbjct: 344 FLQENPDMFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNISFVSFR 403

Query: 373 EATIDVEINA---------------------------------------TPGAFTPFGLG 389
           +AT DV +N                                          G F  FGLG
Sbjct: 404 QATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTFLAFGLG 463

BLAST of Lsi07G012210 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.2e-76
Identity = 170/477 (35.64%), Postives = 255/477 (53.46%), Query Frame = 0

Query: 8   VLIFAALVLVYGVLKRLNGWYYEAKVGK-LWPKLPPGHMGWPLLGSTLSYLKNFSSGQPR 67
           V+   A+V +   ++  + WY+ A +G     +LPPG MGWPL+G   ++L+ F SG+P 
Sbjct: 12  VVAALAVVALDAAVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFKSGRPD 71

Query: 68  NVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKS 127
           + I + + R+G++ +Y+A +    +I+  T E C+QVL D+D F    P++   L G KS
Sbjct: 72  SFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKS 131

Query: 128 LMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASM--KRPIELLTEIK 187
            + +   EHRRLR+LT API+G  AL  Y+  I++TV++ L  W+        E LTE++
Sbjct: 132 FVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELR 191

Query: 188 KLTFKIIWNIFMGSSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKELLNKL 247
           ++TF+II  IFMG +   + AE+E  + ++  G  ++ I+ PGF++HK+++AR+ L+  L
Sbjct: 192 RMTFRIIVQIFMGGADERTAAELERTYTELNYGMRAMAIDLPGFAYHKAIRARRRLVAAL 251

Query: 248 QCIINEKRLVKKREGESWEAK-DMMDLLIELKDEDGEELDDETIIDLIFGKLFAGHETSA 307
           Q +++E+R    +      A  DMMD LI ++DE G  L D+ IID++   L AGHE+S 
Sbjct: 252 QRVLDERRARGGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEIIDVLVMYLNAGHESSG 311

Query: 308 YTAMWAVLFLTNHPHIFQKAK------------------------------VIDETLRVG 367
           +  MWA +FL  +P I  KAK                              V+DETLR  
Sbjct: 312 HITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLSQVVDETLRFV 371

Query: 368 GITFMVFREATIDVEINA---------------------------------------TPG 412
            I+F+ FR+AT DV +N                                          G
Sbjct: 372 NISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRWEGPPPRAG 431

BLAST of Lsi07G012210 vs. ExPASy TrEMBL
Match: A0A1S3BJR1 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490801 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.6e-184
Identity = 340/460 (73.91%), Postives = 366/460 (79.57%), Query Frame = 0

Query: 1   MELKNCLVLIFAALVLVYGVLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELK  LVLI  +L L++GV+KRLNGWYYEAK+GKLWPKLPPG MGWP+LGSTLS+LKNF
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
           +SGQPR ++HNLSIRYGKSDMYK HLLGRASIIVCTSE+CRQVL DE+KFKPSLPRSIT+
Sbjct: 61  NSGQPRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSITL 120

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           LSGRKSLMQVFK EHRRLRRLT APISGH ALEMYINHIE TVISGLEEWASMK+PIELL
Sbjct: 121 LSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELL 180

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKE 240
           TEIKKLTFKIIWNIFMG SS+ SSI EIEALFA++A+GFISLPINFPGFSFHKSLKARKE
Sbjct: 181 TEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARKE 240

Query: 241 LLNKLQCIINEKRLVKKREGESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGH 300
           LL+ LQ IIN+KR+ KK+EGE+WEAKDMMDLLIELKDEDGEELDDETI DLIFGKLFAGH
Sbjct: 241 LLSLLQSIINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAGH 300

Query: 301 ETSAYTAMWAVLFLTNHPHIFQKAK------------------------------VIDET 360
           ETSAYTAMWAVLFLTNHPHI Q+ K                              VIDET
Sbjct: 301 ETSAYTAMWAVLFLTNHPHILQRTKEEQEDIVKRRPTTQKGLSLSEVKQMKFLYQVIDET 360

Query: 361 LRVGGITFMVFREATIDVEIN--------------------------------------- 391
           LRVG ITFMVFREA +DVEIN                                       
Sbjct: 361 LRVGSITFMVFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKQFPSPHEFNPSRWDNFV 420

BLAST of Lsi07G012210 vs. ExPASy TrEMBL
Match: A0A5D3CIW4 (Beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001790 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.4e-182
Identity = 340/464 (73.28%), Postives = 366/464 (78.88%), Query Frame = 0

Query: 1   MELKNCLVLIFAALVLVYGVLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELK  LVLI  +L L++GV+KRLNGWYYEAK+GKLWPKLPPG MGWP+LGSTLS+LKNF
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  SSGQPRNVIHNLSI-RYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSIT 120
           +SGQPR ++HNLSI RYGKSDMYK HLLGRASIIVCTSE+CRQVL DE+KFKPSLPRSIT
Sbjct: 61  NSGQPRILLHNLSISRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSIT 120

Query: 121 ILSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIEL 180
           +LSGRKSLMQVFK EHRRLRRLT APISGH ALEMYINHIE TVISGLEEWASMK+PIEL
Sbjct: 121 LLSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIEL 180

Query: 181 LTEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARK 240
           LTEIKKLTFKIIWNIFMG SS+ SSI EIEALFA++A+GFISLPINFPGFSFHKSLKARK
Sbjct: 181 LTEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARK 240

Query: 241 ELLNKLQCIINEKRLVKKREGESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAG 300
           ELL+ LQ IIN+KR+ KK+EGE+WEAKDMMDLLIELKDEDGEELDDETI DLIFGKLFAG
Sbjct: 241 ELLSLLQSIINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAG 300

Query: 301 HETSAYTAMWAVLFLTNHPHIFQKAK------------------------------VIDE 360
           HETSAYTAMWAVLFLTNHPHI Q+ K                              VIDE
Sbjct: 301 HETSAYTAMWAVLFLTNHPHILQRTKEEQEDIVKRRPTTQKGLSLSEVKQMKFLYQVIDE 360

Query: 361 TLRVGGITFMVFREATIDVEIN-------------------------------------- 391
           TLRVG ITFMVFREA +DVEIN                                      
Sbjct: 361 TLRVGSITFMVFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKQFPSPHEQHDQSLCSV 420

BLAST of Lsi07G012210 vs. ExPASy TrEMBL
Match: A0A6J1L1N7 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500298 PE=3 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 2.5e-171
Identity = 316/460 (68.70%), Postives = 353/460 (76.74%), Query Frame = 0

Query: 1   MELKNCLVLIFAALVLVYGVLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           ME+KN LVLI AA V V G+LKRLNGWYY AK+GK WPKLPPG MGWPLLGST+SY+KNF
Sbjct: 4   MEVKNWLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYIKNF 63

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
            SGQP N I N  IRYGK DMYK HLLGRA+IIVC+SELCRQVL DED FKPSLP++I +
Sbjct: 64  ISGQPSNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNINM 123

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           L+GRKSL+QVFK EH+RLRRLT APISGH ALEMYINHIE+TV+SGLEEWASMK PIELL
Sbjct: 124 LTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELL 183

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKE 240
           +EIKKLTFKIIWNIFMG SS+ SSI E+E LF++VA+GFISLPINFPGF+ HK+LKARK+
Sbjct: 184 SEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKK 243

Query: 241 LLNKLQCIINEKRLVKKREGESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGH 300
           LL+ LQ II++KRLV+KR+G SWEAKDMMDLL+E+KDEDGEELDDETI DLIFGKLFAGH
Sbjct: 244 LLDILQSIIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGH 303

Query: 301 ETSAYTAMWAVLFLTNHPHIFQKAK------------------------------VIDET 360
           ETSAYTAMWAVLFLT HPHIFQ+AK                              +IDET
Sbjct: 304 ETSAYTAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQIIDET 363

Query: 361 LRVGGITFMVFREATIDVEIN--------------------------------------- 391
           LRVGG+T M+FREA +DVEIN                                       
Sbjct: 364 LRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWDNYV 423

BLAST of Lsi07G012210 vs. ExPASy TrEMBL
Match: A0A6J1E938 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431020 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.6e-168
Identity = 311/460 (67.61%), Postives = 350/460 (76.09%), Query Frame = 0

Query: 1   MELKNCLVLIFAALVLVYGVLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           M +KN LVLI AA V V G+LKR+NGWYY AK+GK W  LPPG MGWPLLGST+SY+K+F
Sbjct: 4   MGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYIKSF 63

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
            SGQP N I N  IRYGK DMYK H+LGRA+IIVC+SELCRQVL DED FKPSLP++I +
Sbjct: 64  ISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINM 123

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           L+GRKSL+QVFK EH+RLRRLT APISGH ALEMYINHIE+TV+SGLEEWASMK PIE+L
Sbjct: 124 LTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIEVL 183

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKE 240
           +EIKKLTFKIIWNIFMG SS+ SSI E+E LF++VA+GFISLPINFPGF+ HK+LKARK+
Sbjct: 184 SEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKK 243

Query: 241 LLNKLQCIINEKRLVKKREGESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGH 300
           LLN LQ IIN+KRLV++R+G SWEAKDMMDLL+E+KDEDGEELDDETI DLIFGKLFAGH
Sbjct: 244 LLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGH 303

Query: 301 ETSAYTAMWAVLFLTNHPHIFQKAK------------------------------VIDET 360
           ETSAYTAMWAVLFLT HPHIFQ+AK                              VIDET
Sbjct: 304 ETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDET 363

Query: 361 LRVGGITFMVFREATIDVEIN--------------------------------------- 391
           LRVGG+T M+FREA +DVEIN                                       
Sbjct: 364 LRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWDNYV 423

BLAST of Lsi07G012210 vs. ExPASy TrEMBL
Match: A0A1S3BKD0 (beta-amyrin 11-oxidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490801 PE=4 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 9.2e-166
Identity = 302/381 (79.27%), Postives = 327/381 (85.83%), Query Frame = 0

Query: 1   MELKNCLVLIFAALVLVYGVLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELK  LVLI  +L L++GV+KRLNGWYYEAK+GKLWPKLPPG MGWP+LGSTLS+LKNF
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
           +SGQPR ++HNLSIRYGKSDMYK HLLGRASIIVCTSE+CRQVL DE+KFKPSLPRSIT+
Sbjct: 61  NSGQPRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSITL 120

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           LSGRKSLMQVFK EHRRLRRLT APISGH ALEMYINHIE TVISGLEEWASMK+PIELL
Sbjct: 121 LSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELL 180

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKE 240
           TEIKKLTFKIIWNIFMG SS+ SSI EIEALFA++A+GFISLPINFPGFSFHKSLKARKE
Sbjct: 181 TEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARKE 240

Query: 241 LLNKLQCIINEKRLVKKREGESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGH 300
           LL+ LQ IIN+KR+ KK+EGE+WEAKDMMDLLIELKDEDGEELDDETI DLIFGKLFAGH
Sbjct: 241 LLSLLQSIINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAGH 300

Query: 301 ETSAYTAMWAVLFLTNHPHIFQKAK------------------------------VIDET 351
           ETSAYTAMWAVLFLTNHPHI Q+ K                              VIDET
Sbjct: 301 ETSAYTAMWAVLFLTNHPHILQRTKEEQEDIVKRRPTTQKGLSLSEVKQMKFLYQVIDET 360

BLAST of Lsi07G012210 vs. NCBI nr
Match: XP_008448712.1 (PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 654.8 bits (1688), Expect = 5.3e-184
Identity = 340/460 (73.91%), Postives = 366/460 (79.57%), Query Frame = 0

Query: 1   MELKNCLVLIFAALVLVYGVLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELK  LVLI  +L L++GV+KRLNGWYYEAK+GKLWPKLPPG MGWP+LGSTLS+LKNF
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
           +SGQPR ++HNLSIRYGKSDMYK HLLGRASIIVCTSE+CRQVL DE+KFKPSLPRSIT+
Sbjct: 61  NSGQPRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSITL 120

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           LSGRKSLMQVFK EHRRLRRLT APISGH ALEMYINHIE TVISGLEEWASMK+PIELL
Sbjct: 121 LSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELL 180

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKE 240
           TEIKKLTFKIIWNIFMG SS+ SSI EIEALFA++A+GFISLPINFPGFSFHKSLKARKE
Sbjct: 181 TEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARKE 240

Query: 241 LLNKLQCIINEKRLVKKREGESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGH 300
           LL+ LQ IIN+KR+ KK+EGE+WEAKDMMDLLIELKDEDGEELDDETI DLIFGKLFAGH
Sbjct: 241 LLSLLQSIINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAGH 300

Query: 301 ETSAYTAMWAVLFLTNHPHIFQKAK------------------------------VIDET 360
           ETSAYTAMWAVLFLTNHPHI Q+ K                              VIDET
Sbjct: 301 ETSAYTAMWAVLFLTNHPHILQRTKEEQEDIVKRRPTTQKGLSLSEVKQMKFLYQVIDET 360

Query: 361 LRVGGITFMVFREATIDVEIN--------------------------------------- 391
           LRVG ITFMVFREA +DVEIN                                       
Sbjct: 361 LRVGSITFMVFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKQFPSPHEFNPSRWDNFV 420

BLAST of Lsi07G012210 vs. NCBI nr
Match: XP_038905890.1 (beta-amyrin 11-oxidase-like [Benincasa hispida] >XP_038905891.1 beta-amyrin 11-oxidase-like [Benincasa hispida])

HSP 1 Score: 649.8 bits (1675), Expect = 1.7e-182
Identity = 340/460 (73.91%), Postives = 364/460 (79.13%), Query Frame = 0

Query: 1   MELKNCLVLIFAALVLVYGVLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELKN LV I AALV V+GVLKRLNGWYYEAK+GKLWPKLPPG MGWPLLGSTLSYLK+F
Sbjct: 1   MELKNWLVWIIAALVFVFGVLKRLNGWYYEAKLGKLWPKLPPGDMGWPLLGSTLSYLKHF 60

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
           +SG PRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVL DEDKFKPSLPRSIT+
Sbjct: 61  TSGHPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLCDEDKFKPSLPRSITL 120

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           LSGRKSLMQVFK EHRRLRRLTT  ISGH+ LEMYIN+I++TVI+GLEEWASMK+PIELL
Sbjct: 121 LSGRKSLMQVFKAEHRRLRRLTTGLISGHMVLEMYINYIQHTVINGLEEWASMKKPIELL 180

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKE 240
           TEIKKLTFKIIWNIFMG SS+ SSI EIE LF K A+GFISLPINFPGF FHKSLK RK+
Sbjct: 181 TEIKKLTFKIIWNIFMGSSSIDSSIGEIEPLFTKTALGFISLPINFPGFIFHKSLKGRKQ 240

Query: 241 LLNKLQCIINEKRLVKKREGESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGH 300
           LL  LQ IIN+KRLVK+REG+ WEAKDMMDLL+ELKDE+GEELDDETI DLIFGKLFAGH
Sbjct: 241 LLTILQSIINQKRLVKEREGKKWEAKDMMDLLMELKDEEGEELDDETIKDLIFGKLFAGH 300

Query: 301 ETSAYTAMWAVLFLTNHPHIFQKAK------------------------------VIDET 360
           ETSAYTAMWA+LFLTNHPHIFQKAK                              VIDET
Sbjct: 301 ETSAYTAMWAILFLTNHPHIFQKAKEEQEDIIKRRPTTQKGITLSEVKQMKFLYQVIDET 360

Query: 361 LRVGGITFMVFREATIDVEIN--------------------------------------- 391
           LRV GITFMVFREA +DVEIN                                       
Sbjct: 361 LRVSGITFMVFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKQFPSPREFNPSRWDNFV 420

BLAST of Lsi07G012210 vs. NCBI nr
Match: XP_011650352.2 (beta-amyrin 11-oxidase [Cucumis sativus] >KAE8650035.1 hypothetical protein Csa_011096 [Cucumis sativus])

HSP 1 Score: 649.8 bits (1675), Expect = 1.7e-182
Identity = 341/460 (74.13%), Postives = 359/460 (78.04%), Query Frame = 0

Query: 1   MELKNCLVLIFAALVLVYGVLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELK  LVLI   L L++GV+KRLNGWYYEAK+GKLWPKLPPG MGWP+LG TLSYLKNF
Sbjct: 1   MELKEWLVLITGGLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGVTLSYLKNF 60

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
           SSGQPR ++HNLSIRYGKSDMYK HLLGRASIIVCTSE+CRQVL DE+KFKPSLPRSIT+
Sbjct: 61  SSGQPRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSITL 120

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           LSGRKSLMQVFK EHRRLRRLTTAPISGH ALEMYINHIE TVISGLEEWASMKRPIELL
Sbjct: 121 LSGRKSLMQVFKAEHRRLRRLTTAPISGHSALEMYINHIEQTVISGLEEWASMKRPIELL 180

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKE 240
           TEIKKLTFKIIWNIFMG SS+ SSI EIEALFAK+ +GFISLPIN PGFSF+KSLKARKE
Sbjct: 181 TEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAKITLGFISLPINLPGFSFYKSLKARKE 240

Query: 241 LLNKLQCIINEKRLVKKREGESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGH 300
           LL  LQ IIN+KR+ KKREGE WEAKDMMDLLIELKDEDGEELDDETI DLIFGKLFAGH
Sbjct: 241 LLTILQSIINQKRMAKKREGEGWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAGH 300

Query: 301 ETSAYTAMWAVLFLTNHPHIFQKAK------------------------------VIDET 360
           ETSAYTAMWAVLFLTNHPHI QKAK                              VIDET
Sbjct: 301 ETSAYTAMWAVLFLTNHPHILQKAKEEQEDIIKRRPTTQKGLILSEVKQMKFLYQVIDET 360

Query: 361 LRVGGITFMVFREATIDVEIN--------------------------------------- 391
           LR   ITFMVFREA +DVEIN                                       
Sbjct: 361 LRAACITFMVFREAVVDVEINGKTIPKGWKVIPWFGELYMDEKQFPSPHEFNPSRWDNFV 420

BLAST of Lsi07G012210 vs. NCBI nr
Match: KAA0053060.1 (beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo var. makuwa] >TYK11515.1 beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 649.0 bits (1673), Expect = 2.9e-182
Identity = 340/464 (73.28%), Postives = 366/464 (78.88%), Query Frame = 0

Query: 1   MELKNCLVLIFAALVLVYGVLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELK  LVLI  +L L++GV+KRLNGWYYEAK+GKLWPKLPPG MGWP+LGSTLS+LKNF
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  SSGQPRNVIHNLSI-RYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSIT 120
           +SGQPR ++HNLSI RYGKSDMYK HLLGRASIIVCTSE+CRQVL DE+KFKPSLPRSIT
Sbjct: 61  NSGQPRILLHNLSISRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSIT 120

Query: 121 ILSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIEL 180
           +LSGRKSLMQVFK EHRRLRRLT APISGH ALEMYINHIE TVISGLEEWASMK+PIEL
Sbjct: 121 LLSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIEL 180

Query: 181 LTEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARK 240
           LTEIKKLTFKIIWNIFMG SS+ SSI EIEALFA++A+GFISLPINFPGFSFHKSLKARK
Sbjct: 181 LTEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARK 240

Query: 241 ELLNKLQCIINEKRLVKKREGESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAG 300
           ELL+ LQ IIN+KR+ KK+EGE+WEAKDMMDLLIELKDEDGEELDDETI DLIFGKLFAG
Sbjct: 241 ELLSLLQSIINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAG 300

Query: 301 HETSAYTAMWAVLFLTNHPHIFQKAK------------------------------VIDE 360
           HETSAYTAMWAVLFLTNHPHI Q+ K                              VIDE
Sbjct: 301 HETSAYTAMWAVLFLTNHPHILQRTKEEQEDIVKRRPTTQKGLSLSEVKQMKFLYQVIDE 360

Query: 361 TLRVGGITFMVFREATIDVEIN-------------------------------------- 391
           TLRVG ITFMVFREA +DVEIN                                      
Sbjct: 361 TLRVGSITFMVFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKQFPSPHEQHDQSLCSV 420

BLAST of Lsi07G012210 vs. NCBI nr
Match: XP_023552542.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 614.4 bits (1583), Expect = 7.9e-172
Identity = 315/460 (68.48%), Postives = 355/460 (77.17%), Query Frame = 0

Query: 1   MELKNCLVLIFAALVLVYGVLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           ME+KN LVLI AA V V G+LKR+NGWYY AK+GK WPKLPPGHMGWPLLGST+SY+KNF
Sbjct: 4   MEVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWPKLPPGHMGWPLLGSTISYIKNF 63

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
            SGQP N + N  IRYGKSDMYK H+LGRA+IIVC+SELCRQVL DED FKPSLP++I +
Sbjct: 64  ISGQPSNFMANFYIRYGKSDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINM 123

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           L+GRKSL+QVFK EH+RLRRLT APISGH ALEMYINHIE+TV+SGLEEWASMK PIELL
Sbjct: 124 LTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELL 183

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKE 240
           +EIKKLTFKIIWNIFMG SS+ SSI E+E LF++VA+GFISLPINFPGF+ HK+LKARK+
Sbjct: 184 SEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKK 243

Query: 241 LLNKLQCIINEKRLVKKREGESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGH 300
           LL+ LQ IIN+KRLV++R+G SWEAKDMMDLL+E+KDEDGEELDDETI DLIFGKLFAGH
Sbjct: 244 LLDILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGH 303

Query: 301 ETSAYTAMWAVLFLTNHPHIFQKAK------------------------------VIDET 360
           ETSAYTAMWAVLFLT HPHIFQ+AK                              +IDET
Sbjct: 304 ETSAYTAMWAVLFLTKHPHIFQRAKEEQDRIITQRPTTQKGIILSEVKQMKFLSQIIDET 363

Query: 361 LRVGGITFMVFREATIDVEIN--------------------------------------- 391
           LRVGG+T M+FREA +DVEIN                                       
Sbjct: 364 LRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWDNYV 423

BLAST of Lsi07G012210 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 323.6 bits (828), Expect = 2.6e-88
Identity = 186/464 (40.09%), Postives = 257/464 (55.39%), Query Frame = 0

Query: 20  VLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNVIHNLSIRYGKS 79
           +++++N W YE+ +G+    LPPG +GWP +G+ LS+L+ F +  P +    L  RYG  
Sbjct: 25  LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84

Query: 80  DMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLMQVFKGEHRRLR 139
            +YKAH+ G  SIIV TS+ CR+VL+D+D FKP  P S   L GRKS + +   EH+RLR
Sbjct: 85  GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144

Query: 140 RLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTFKIIWNIFMGSS 199
           RLT AP++GH AL  YI +IE  VI+ L++W  M    E LT ++KLTF+II  IF+ S 
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204

Query: 200 VHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKELLNKLQCIINEKRLVKKREG 259
             + +  +E  +  +  G  ++ +N PGF++H++LKARK L+   Q I+ E+R  +K+  
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNI 264

Query: 260 ESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGHETSAYTAMWAVLFLTNHPHI 319
            S   KDM+D L+ +KDEDG+ LDDE IID++   L AGHE+S +T MWA +FL  HP +
Sbjct: 265 LS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 324

Query: 320 FQKAK------------------------------VIDETLRVGGITFMVFREATIDVEI 379
            Q+AK                              V+DETLRV   +   FREA  DVE+
Sbjct: 325 LQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVEM 384

Query: 380 NA----------------------------------------TPGAFTPFGLGSRYCPGS 413
           N                                           GAF PFG GS  CPG+
Sbjct: 385 NGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLCPGN 444

BLAST of Lsi07G012210 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 317.4 bits (812), Expect = 1.9e-86
Identity = 186/471 (39.49%), Postives = 261/471 (55.41%), Query Frame = 0

Query: 9   LIFAALVLVYGVLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNV 68
           LI   L ++  VLKR+N W Y +K+G+    LPPG +GWP++G+  S+L+ F +  P + 
Sbjct: 13  LIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESF 72

Query: 69  IHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLM 128
           I +   RYG++ +YKAH+ G   ++V T E CR+VL+D+D F    P+S   L GRKS +
Sbjct: 73  IQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFV 132

Query: 129 QVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTF 188
            +   EH+RLRRLT+AP++G  AL +YI  IE TV + LE+W+ M   IE L+ ++KLTF
Sbjct: 133 GISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTF 192

Query: 189 KIIWNIFMGSSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKELLNKLQCII 248
           K+I  IF+ S     +  +E  +  +  G  ++ IN PGF++H++LKARK+L+   Q I+
Sbjct: 193 KVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIV 252

Query: 249 NEKRLVKKREGESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGHETSAYTAMW 308
             +R  +K+   S   KDM+D LI++KDE+G  LDDE IIDL+   L AGHE+S +  MW
Sbjct: 253 TNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMW 312

Query: 309 AVLFLTNHPHIFQKAK-----------------------------VIDETLRVGGITFMV 368
           A + +  HP I QKAK                             VIDETLRV   +   
Sbjct: 313 ATILMQEHPMILQKAKEEQERIVKKRAPGQKLTLKETREMVYLSQVIDETLRVITFSLTA 372

Query: 369 FREATIDVEINA-------------------------------------TP--GAFTPFG 412
           FREA  DV+++                                      TP  G F PFG
Sbjct: 373 FREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFG 432

BLAST of Lsi07G012210 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 317.4 bits (812), Expect = 1.9e-86
Identity = 186/471 (39.49%), Postives = 261/471 (55.41%), Query Frame = 0

Query: 9   LIFAALVLVYGVLKRLNGWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNV 68
           LI   L ++  VLKR+N W Y +K+G+    LPPG +GWP++G+  S+L+ F +  P + 
Sbjct: 13  LIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESF 72

Query: 69  IHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLM 128
           I +   RYG++ +YKAH+ G   ++V T E CR+VL+D+D F    P+S   L GRKS +
Sbjct: 73  IQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFV 132

Query: 129 QVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTF 188
            +   EH+RLRRLT+AP++G  AL +YI  IE TV + LE+W+ M   IE L+ ++KLTF
Sbjct: 133 GISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTF 192

Query: 189 KIIWNIFMGSSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKARKELLNKLQCII 248
           K+I  IF+ S     +  +E  +  +  G  ++ IN PGF++H++LKARK+L+   Q I+
Sbjct: 193 KVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIV 252

Query: 249 NEKRLVKKREGESWEAKDMMDLLIELKDEDGEELDDETIIDLIFGKLFAGHETSAYTAMW 308
             +R  +K+   S   KDM+D LI++KDE+G  LDDE IIDL+   L AGHE+S +  MW
Sbjct: 253 TNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMW 312

Query: 309 AVLFLTNHPHIFQKAK-----------------------------VIDETLRVGGITFMV 368
           A + +  HP I QKAK                             VIDETLRV   +   
Sbjct: 313 ATILMQEHPMILQKAKEEQERIVKKRAPGQKLTLKETREMVYLSQVIDETLRVITFSLTA 372

Query: 369 FREATIDVEINA-------------------------------------TP--GAFTPFG 412
           FREA  DV+++                                      TP  G F PFG
Sbjct: 373 FREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFG 432

BLAST of Lsi07G012210 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 163.3 bits (412), Expect = 4.5e-40
Identity = 111/422 (26.30%), Postives = 191/422 (45.26%), Query Frame = 0

Query: 38  PKLPPGHMGWPLLGSTLSYLKNFSSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTS 97
           PK P G +GWP++G T+ ++ +  S +P + +    + YG+  ++K+H+ G A+I+   +
Sbjct: 49  PKFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGR--VFKSHIFGTATIVSTDA 108

Query: 98  ELCRQVL-SDEDKFKPSLPRSITILSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYI 157
           E+ R VL SD   F P  P+++  L G+ S++ +    HRR   L  + +   +     +
Sbjct: 109 EVNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIV 168

Query: 158 NHIENTVISGLEEWASMKRPIELLTEIKKLTFKIIWNIFMGSSVHSSIAEIEALFAKVAV 217
             +   +   ++ W S  +P+ L    K + FK++    +       + E++  F     
Sbjct: 169 RDMHKFLSESMDLW-SEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFIS 228

Query: 218 GFISLPINFPGFSFHKSLKARKELLNKLQCIINEK-RLVKKREGESWEAKDMMDLLIELK 277
           G +SLPINFPG   H+SL+A+K ++ +++ II  K R  K +E +   AKD++D+L++  
Sbjct: 229 GLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLK-- 288

Query: 278 DEDGEELDDETIIDLIFGKLFAGHETSAYTAMWAVLFLTNHPHIFQ-------------- 337
            +  E L    I + +   +  GH++       AV FL++ P                  
Sbjct: 289 -DSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKE 348

Query: 338 ----------------KAKVIDETLRVGGITFMVFREATIDVEI---------------- 389
                             KVI ETLR+G +   V R+A  DVEI                
Sbjct: 349 LTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLR 408

BLAST of Lsi07G012210 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 153.7 bits (387), Expect = 3.6e-37
Identity = 131/424 (30.90%), Postives = 190/424 (44.81%), Query Frame = 0

Query: 39  KLPPGHMGWPLLGSTLSYLKNFSSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSE 98
           KLPPG MGWP LG TL       S  P     +   RYG  +++K  +LG   +++ + E
Sbjct: 33  KLPPGSMGWPYLGETL----QLYSQNPNVFFTSKQKRYG--EIFKTRILGYPCVMLASPE 92

Query: 99  LCRQVL-SDEDKFKPSLPRSITILSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYIN 158
             R VL +    FKP+ PRS   L G  +L       H  +R+L  +       +   I 
Sbjct: 93  AARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSFYPE-TIRKLIP 152

Query: 159 HIENTVISGLEEWASMKRPIELLTEIKKLTFKI-IWNIFMGSSVHSSIAEI-EALFAKVA 218
            IE+  +S L+ WA+M   +    E+KK  F + I  IF    + SS  EI +  +  V 
Sbjct: 153 DIEHIALSSLQSWANMP-IVSTYQEMKKFAFDVGILAIF--GHLESSYKEILKHNYNIVD 212

Query: 219 VGFISLPINFPGFSFHKSLKARKELLNKLQCIINEKRLVKKREGESWEAKDMMDLLIELK 278
            G+ S P++ PG S+HK+L ARK+L   +  II E+R  +KR  ++    D +  L+  K
Sbjct: 213 KGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERR--EKRALQT----DFLGHLLNFK 272

Query: 279 DEDGEELDDETIIDLIFGKLFAGHETSAYTAMWAVLFLTNHPHIF------QKA------ 338
           +E G  L  E I D I G LFA  +T+A    W + +L +   +       QKA      
Sbjct: 273 NEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAEQKAIYEENS 332

Query: 339 ------------------KVIDETLRVGGITFMVFREATIDV------------------ 392
                             KVI E+LR+  I    FREA +DV                  
Sbjct: 333 REKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFR 392