Lsi07G011300 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G011300
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionATP-dependent Clp protease proteolytic subunit
Locationchr07: 16740003 .. 16744302 (+)
RNA-Seq ExpressionLsi07G011300
SyntenyLsi07G011300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTGGGGCATTAGTGCTATTTTGATAAATGCATGGGTGGATTATTGAAATTTCCAAATTTCCCAAACTTTTAGCAATCTGTATTTCTCACCCAACCAAATCCCCAAAGTCATCGTCTCTCCGATTCTTCCTGGTTTCAGAGCTGAAGCAGAGCAGTGCTGTAAATCCATGGAGCTTCTTTCACGCTGTTCTACTCTTATTCCTCACGCCTCCTCTCTCGGCCTCCCAAATTCTCATGGAAAACCATCAAATTTCTTCCCCAAGCTCAAATCCACTCTCTCCTTTTCTTCGGCTTCATCAGTCCTCAAAACAACCTCTCTTAAACCCTCGAGAACCCTAGCTCCACCGTGCTCCGTCCTTATGACGGCTCCACAGACGCCGGATGCCGCCCGTAGAGGCGCCGAGACTGACGCCATGGGCCTGCTTCTCAGGGAGAGGATCGTCTTCTTGGGGAACAGCATTGATGACTTTGTGGCCGATGCTATTATCAGTCAGCTCTTGCTCTTGGATGCTCAGGACTCTACTAAAGATATCAGACTCTTCATTAATTCCGCTGGCGGCTCTCTCAGGTATTCACGAGTTGATTTTTTGTGCATAATGTAGTTGGTAAAGATATGGAATATGTGATTAGGGCTCAGTTTTATTCTTTCCAAACGATTTAGGTAATTTAATTTCTATTAATATGCCTAATTTTCTTTAGAGTTTTATTCACCTGCATTGCAAACAGTTAGCCCACTCGTTGGTGCATTCATTCCCGTGACTATTCTGGTTCTAGAGTTGACATTGATAAAACAAGAGATTTCTTTGATCAGTGCCCTTTAAAAGAATGGCAATGGTTGGAGTTTTAAATTTAGGAGCAGGACAAACACCTGGCTTCATCCTTCCCGTCTAAGTTATATTTTCCAAACATTTTCTGAATTTCCAAATGCTAAAGATTTTTGGAATGGGGCAATCTGAACTTGGGATTTTCAGCTCAGAAGAAACCCCTTTGACTTGATAGAGAGCCATAAGAATGGTTGACATTTGTTCAAAAGATGGAAAGCAATCAGCTAAATCTTACTATCTTAGTAAAGATAAATTTTGTTGGAAGTTTGAAACCTTTGTCTTGTATCCACTTGCAAATCTTCCAACCTCCAGCTGAATTGTAGAGAAAGCAGGTTGGATGAACCCTTTTTATCTTCGGTTTGGGGAGGAAAAGACTTCGTTCTTCATTTGGACTATGGCTTACAAGAGCCTAAACACTTGTGATTGGATTAAAAAGAAGAACCCTTCCATGGCTTTATCTCCTTATGTTTGCCCTTTCTTTTCCTCTTTTTTTTGAAACGGAAACAGAACTTTTCATTGATATAATGAAAAGAGCAAAGGCTCCAATTACAATGAGACAAAACCAAATAAGCTAAGAAATTTAGAAACATAGGATCAGGAGGTGCATCCGGATATCTCAACTAGGTTGACACAGCCCTAGCACCCTCATCATTTTCCTATACAAAATACAAAAATTCAGAAAAAGCCAATATTCTTAGCTACTTGGTTACATAGAAATAATAAGAGCATTCCAATTGAGATAAATGTCCTAAATAGAATAATCTTCAAAGAGTTTGGACTGGGAGCTCCAAGAAGAAGCTAAGAGATGGGTTGAACCCCATCTGTCTAACCAATCCTTTGAACTATCATGGAAGACTCTCTAATTACGTTCAAACCAAATCTCAGAAATCAAGGCTTTGACTGCATTTGTCCATAATAACTGTGAGCTCTAGAAGAGTGAGAAGGACCAACCAAAAGATGAGAGATATTATCCGTAACTGTGTTGCCCAAAACCCATTGAAAACCAAAGCAAGAAAATAATTTCCACCAACAATTTCTGGAGAAAGAGCAATCAAATAACAAGTGATGCAAATCCTCTCCGTTTGCTGAACATAGAGGACAAATTGAGGGTGAAAGATATTTATTTCGAAGCTTATTTTGCAAAGTAAATGAACAATTGAGGGAACCAAAAGTTTAGCCAATCAAAAACTCCATTTTCTATAATGATCAATTTCCCAAATCTCCTTTGGAAAATGACTAACACTCCTCCTCCTAAGTGAGTGAGCATGTTGGCCAAATAAAAATCTTTTCAACAATTAAACATCCAGGCCTTCTTGTTCAATCCCTATGACATTCTCAGTGGCAGGTGATAAAGCATAGAGGTGGTGGAAGTTTAAGATACTAAAGGTTCCCTAATTACTAAAGTATGGTAGTAATGCATCTATTACCCGTCTCTCCCTTTTCATTTGTGGTATATTTTGAATAACTTGCTAGGAGTTATGAAAAAGAACTGAATTCAGAATGTAAAAACATCTACATATTATTATCTATTTGTATCAAGTTCTATGTTGCACTGGATAACACTTGGTCCCACACAGTATAATTTTATCTTGAAAGCATGTAATCTATATATATAATATGCAACCAATCTTTACATCTAATTTTTTGCATTTGAATGAGAACTGAATATTTCTTTTCATACTTCAATGGAGAAATGTAATGTGCTTGTCTATGGCTAGAGATGAGTGTATTCGTCTACTTTACTTTACTACATGAATGGGAAGATTAGCCAACTGTGAGAACTATACATATCTTCCCTGATTGTTAATTTATTTCTGTTTTATTCTTGTTTCGATGCTTTCCATGGCCTTCATGTACATAGGAAATCTTCTAGAGTAATTTAATTTAGATTTCTGTGGTTTATGGATCACCTTTTCATAATCTTCTCTTGGCATCTTTGATGTGCAACCAGTTTCTAATGGTAAATCTATCATTCACTACCAAACCATCATTTTTCGAAATGTATATTGATATGTAACTTAAGGATGCTCGTGAGACTTGAGAGGCGTCAACAGAAGAAACTACGTACTTTGCCTAACCCAGTTCATTTTTCAGAAAATGTTATGTTTTTAAGTTCCACGTCCACTTATCCTCATGTTGATTAAGGAACACACTTCCAACTATCTTGTAATCCAAACCCTGTCTTCCAATTGTCTATCAAACAGATTCCTTCCCGGTCCAATGGAGTAGATATTATGAAATACCTGACTCAAAGGTGGAATCAAACCAGTTATCTTCCTAGATTCTAAGCCCGTTTCCCATCTTATAAAGAAAACCCTTTCCCGATGAACCCATACATTTAGCTAGACCAGACCAGGGGCAAAAGGCAGACTAGCTTACGTAGTTTTATTTGTTCTCAGAGCACCAGGACTTAAAATTTTAGAAGTGGGAGTGCCATATTCATGACTCACGAGTATATGAATTCTCTTGTCATTGTTTATTTGAAGCTGGAAATATCGATTACTAGGCTTTTTAAATATATATATTTCTGTTTTTCCCTCTCTGGTCCTGAGAAAATTTAGGTTAGGAGGATATTCAGCTTTCTATGAGCGGCACCTGATCTCATTTAATTTTATTTTGAAAGTGGATGGTCTTCTTAGTTAATATTTGGTGTTTGGTTGCAGTACATTAACCTTGACTGATTTCCTTCTATTAATTTCGTTTTCATTAGTGCTACCATGGCTATCTACGATGTCGTACAGCTCGTGAGGGCTGATGTCTCCACTATTGCACTAGGCATTGCAGCCTCAACAGCTTCCATAATCCTCGGTGGTGGCACTAAAGGAAAGCGTCTCGCAATGCCTAATGCACGTATTATGGTTCATCAACCTCTTGGAGGGGCGAGTGGGCAAGCAATAGATGTGGAAATTCAAGCACGCGAAATCATGCATAACAAGAACAATGTTACAAGAATCATCTCCGAATTCACTGGCCATCCATTTGAAAAGGTCCAAAAAGATATCGATAGGGATCGTTATATGTCGCCCATAGAGGCTGTAGAATATGGATTGATCGATGGAGTGATTGACAAAGATAGCATTATACCTCTCATGCCAGTGCCAGAAAGAGTGAAAGCAAGTTTAAATTATGAAGAAATTAGTAAAGATCCCAGAAAATTCTTGACACCAGATGTCCCTGACGACGAGATATATTAGTTTCTGGCGTTTCGGCAAACCTTTCTTTAGGCTTTTGATTTATATGATTTACATGTAGGATCTTATGTGTAAGCAAAGTCATGGTAGGTGGAAACTAGAAATATGCTGGAAATTGATGACCTAAACACCTTTAGAGTATCAACTTTTGGCAAATTGTCTCTACTTACCAGAAGTTAAAAGGTATTGGGTATTTGGACTCTTTTTTTGTCTATGACATTTCAAATGAGCATTCTGAGTAAGGAAAAATTGGCATTGAGTCATATTTTTTATTGGCAGTGTAT

mRNA sequence

ATTTGGGGCATTAGTGCTATTTTGATAAATGCATGGGTGGATTATTGAAATTTCCAAATTTCCCAAACTTTTAGCAATCTGTATTTCTCACCCAACCAAATCCCCAAAGTCATCGTCTCTCCGATTCTTCCTGGTTTCAGAGCTGAAGCAGAGCAGTGCTGTAAATCCATGGAGCTTCTTTCACGCTGTTCTACTCTTATTCCTCACGCCTCCTCTCTCGGCCTCCCAAATTCTCATGGAAAACCATCAAATTTCTTCCCCAAGCTCAAATCCACTCTCTCCTTTTCTTCGGCTTCATCAGTCCTCAAAACAACCTCTCTTAAACCCTCGAGAACCCTAGCTCCACCGTGCTCCGTCCTTATGACGGCTCCACAGACGCCGGATGCCGCCCGTAGAGGCGCCGAGACTGACGCCATGGGCCTGCTTCTCAGGGAGAGGATCGTCTTCTTGGGGAACAGCATTGATGACTTTGTGGCCGATGCTATTATCAGTCAGCTCTTGCTCTTGGATGCTCAGGACTCTACTAAAGATATCAGACTCTTCATTAATTCCGCTGGCGGCTCTCTCAGTGCTACCATGGCTATCTACGATGTCGTACAGCTCGTGAGGGCTGATGTCTCCACTATTGCACTAGGCATTGCAGCCTCAACAGCTTCCATAATCCTCGGTGGTGGCACTAAAGGAAAGCGTCTCGCAATGCCTAATGCACGTATTATGGTTCATCAACCTCTTGGAGGGGCGAGTGGGCAAGCAATAGATGTGGAAATTCAAGCACGCGAAATCATGCATAACAAGAACAATGTTACAAGAATCATCTCCGAATTCACTGGCCATCCATTTGAAAAGGTCCAAAAAGATATCGATAGGGATCGTTATATGTCGCCCATAGAGGCTGTAGAATATGGATTGATCGATGGAGTGATTGACAAAGATAGCATTATACCTCTCATGCCAGTGCCAGAAAGAGTGAAAGCAAGTTTAAATTATGAAGAAATTAGTAAAGATCCCAGAAAATTCTTGACACCAGATGTCCCTGACGACGAGATATATTAGTTTCTGGCGTTTCGGCAAACCTTTCTTTAGGCTTTTGATTTATATGATTTACATGTAGGATCTTATGTGTAAGCAAAGTCATGGTAGGTGGAAACTAGAAATATGCTGGAAATTGATGACCTAAACACCTTTAGAGTATCAACTTTTGGCAAATTGTCTCTACTTACCAGAAGTTAAAAGGTATTGGGTATTTGGACTCTTTTTTTGTCTATGACATTTCAAATGAGCATTCTGAGTAAGGAAAAATTGGCATTGAGTCATATTTTTTATTGGCAGTGTAT

Coding sequence (CDS)

ATGGAGCTTCTTTCACGCTGTTCTACTCTTATTCCTCACGCCTCCTCTCTCGGCCTCCCAAATTCTCATGGAAAACCATCAAATTTCTTCCCCAAGCTCAAATCCACTCTCTCCTTTTCTTCGGCTTCATCAGTCCTCAAAACAACCTCTCTTAAACCCTCGAGAACCCTAGCTCCACCGTGCTCCGTCCTTATGACGGCTCCACAGACGCCGGATGCCGCCCGTAGAGGCGCCGAGACTGACGCCATGGGCCTGCTTCTCAGGGAGAGGATCGTCTTCTTGGGGAACAGCATTGATGACTTTGTGGCCGATGCTATTATCAGTCAGCTCTTGCTCTTGGATGCTCAGGACTCTACTAAAGATATCAGACTCTTCATTAATTCCGCTGGCGGCTCTCTCAGTGCTACCATGGCTATCTACGATGTCGTACAGCTCGTGAGGGCTGATGTCTCCACTATTGCACTAGGCATTGCAGCCTCAACAGCTTCCATAATCCTCGGTGGTGGCACTAAAGGAAAGCGTCTCGCAATGCCTAATGCACGTATTATGGTTCATCAACCTCTTGGAGGGGCGAGTGGGCAAGCAATAGATGTGGAAATTCAAGCACGCGAAATCATGCATAACAAGAACAATGTTACAAGAATCATCTCCGAATTCACTGGCCATCCATTTGAAAAGGTCCAAAAAGATATCGATAGGGATCGTTATATGTCGCCCATAGAGGCTGTAGAATATGGATTGATCGATGGAGTGATTGACAAAGATAGCATTATACCTCTCATGCCAGTGCCAGAAAGAGTGAAAGCAAGTTTAAATTATGAAGAAATTAGTAAAGATCCCAGAAAATTCTTGACACCAGATGTCCCTGACGACGAGATATATTAG

Protein sequence

MELLSRCSTLIPHASSLGLPNSHGKPSNFFPKLKSTLSFSSASSVLKTTSLKPSRTLAPPCSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTKDIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNARIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPIEAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY
Homology
BLAST of Lsi07G011300 vs. ExPASy Swiss-Prot
Match: Q94B60 (ATP-dependent Clp protease proteolytic subunit 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLPP4 PE=1 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.0e-101
Identity = 212/299 (70.90%), Postives = 234/299 (78.26%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLGLPNSHGKPSNFFPK-----LKSTLSFSSASSVLKTTSLKPSR 60
           M  LS  S+L P   S  L +S    S+ FPK     LK T      S  L+TTS  P R
Sbjct: 1   MGTLSLSSSLKPSLVSSRLNSSSSASSSSFPKPNNLYLKPT---KLISPPLRTTSPSPLR 60

Query: 61  TLAPPCSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDA 120
                 +  +   QT ++A RGAE+D MGLLLRERIVFLG+SIDDFVADAI+SQLLLLDA
Sbjct: 61  F----ANASIEMSQTQESAIRGAESDVMGLLLRERIVFLGSSIDDFVADAIMSQLLLLDA 120

Query: 121 QDSTKDIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRL 180
           +D  KDI+LFINS GGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILG GTKGKR 
Sbjct: 121 KDPKKDIKLFINSPGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGAGTKGKRF 180

Query: 181 AMPNARIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDR 240
           AMPN RIM+HQPLGGASGQAIDVEIQA+E+MHNKNNVT II+  T   FE+V KDIDRDR
Sbjct: 181 AMPNTRIMIHQPLGGASGQAIDVEIQAKEVMHNKNNVTSIIAGCTSRSFEQVLKDIDRDR 240

Query: 241 YMSPIEAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 295
           YMSPIEAVEYGLIDGVID DSIIPL PVP+RVK  +NYEEISKDP KFLTP++PDDEIY
Sbjct: 241 YMSPIEAVEYGLIDGVIDGDSIIPLEPVPDRVKPRVNYEEISKDPMKFLTPEIPDDEIY 292

BLAST of Lsi07G011300 vs. ExPASy Swiss-Prot
Match: Q3ANI8 (ATP-dependent Clp protease proteolytic subunit 1 OS=Synechococcus sp. (strain CC9605) OX=110662 GN=clpP1 PE=3 SV=2)

HSP 1 Score: 212.2 bits (539), Expect = 7.8e-54
Identity = 106/195 (54.36%), Postives = 143/195 (73.33%), Query Frame = 0

Query: 60  PCSVLMTAPQTPDAARRGAET-DAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDS 119
           P S     P   + + RG    D    LLRERI+FLG  +DD VADA+++Q+L L+A+D 
Sbjct: 2   PVSAPGPLPTVVEQSGRGDRAFDIYSRLLRERIIFLGTGVDDAVADALVAQMLFLEAEDP 61

Query: 120 TKDIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMP 179
            KDI+++INS GGS++A +AIYD +Q V  DV TI  G+AAS  + +L GGTKGKRLA+P
Sbjct: 62  EKDIQIYINSPGGSVTAGLAIYDTMQQVAPDVVTICYGLAASMGAFLLSGGTKGKRLALP 121

Query: 180 NARIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMS 239
           NARIM+HQPLGGA GQA+D+EIQA+EI++ K  +  +++E TG P +K+ +D DRD ++S
Sbjct: 122 NARIMIHQPLGGAQGQAVDIEIQAKEILYLKETLNGLMAEHTGQPLDKISEDTDRDYFLS 181

Query: 240 PIEAVEYGLIDGVID 254
           P EAVEYGLID V+D
Sbjct: 182 PAEAVEYGLIDRVVD 196

BLAST of Lsi07G011300 vs. ExPASy Swiss-Prot
Match: Q3B0U1 (ATP-dependent Clp protease proteolytic subunit 2 OS=Synechococcus sp. (strain CC9902) OX=316279 GN=clpP2 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.7e-53
Identity = 105/195 (53.85%), Postives = 142/195 (72.82%), Query Frame = 0

Query: 60  PCSVLMTAPQTPDAARRGAET-DAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDS 119
           P S     P   + + RG    D    LLRERI+FLG  +DD VADA+++Q+L L+A+D 
Sbjct: 2   PISAPGPLPTVVEQSGRGDRAFDIYSRLLRERIIFLGTGVDDAVADALVAQMLFLEAEDP 61

Query: 120 TKDIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMP 179
            KDI++++NS GGS++A +AIYD +Q V  DV TI  G+AAS  + +L GGTKGKRLA+P
Sbjct: 62  EKDIQIYVNSPGGSVTAGLAIYDTMQQVAPDVVTICYGLAASMGAFLLSGGTKGKRLALP 121

Query: 180 NARIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMS 239
           NARIM+HQPLGGA GQA+D+EIQA+EI+  K  +  +++E TG P +K+ +D DRD ++S
Sbjct: 122 NARIMIHQPLGGAQGQAVDIEIQAKEILFLKETLNGLLAEHTGQPLDKISEDTDRDYFLS 181

Query: 240 PIEAVEYGLIDGVID 254
           P EAVEYGLID V+D
Sbjct: 182 PAEAVEYGLIDRVVD 196

BLAST of Lsi07G011300 vs. ExPASy Swiss-Prot
Match: Q7V992 (ATP-dependent Clp protease proteolytic subunit 1 OS=Prochlorococcus marinus (strain MIT 9313) OX=74547 GN=clpP1 PE=3 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.1e-52
Identity = 103/187 (55.08%), Postives = 139/187 (74.33%), Query Frame = 0

Query: 68  PQTPDAARRGAET-DAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTKDIRLFI 127
           P   + + RG    D    LLRERI+FLG  +DD VADA+++Q+L L+A+D  KDI+++I
Sbjct: 27  PTVVEQSGRGERAFDIYSRLLRERIIFLGTGVDDQVADALVAQMLFLEAEDPEKDIQIYI 86

Query: 128 NSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNARIMVHQ 187
           NS GGS++A +AIYD +Q V  DV TI  G+AAS  + +L GGTKGKRLA+PNARIM+HQ
Sbjct: 87  NSPGGSVTAGLAIYDTMQQVAPDVVTICYGLAASMGAFLLCGGTKGKRLALPNARIMIHQ 146

Query: 188 PLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPIEAVEYG 247
           PLGGA GQA+D+EIQA+EI+  K  +  +++E TG P  K+ +D DRD ++SP +AVEYG
Sbjct: 147 PLGGAQGQAVDIEIQAKEILFLKETLNGLLAEHTGQPLNKIAEDTDRDHFLSPAKAVEYG 206

Query: 248 LIDGVID 254
           LID V+D
Sbjct: 207 LIDRVVD 213

BLAST of Lsi07G011300 vs. ExPASy Swiss-Prot
Match: O34125 (ATP-dependent Clp protease proteolytic subunit 2 OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=clpP2 PE=3 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.5e-52
Identity = 103/191 (53.93%), Postives = 143/191 (74.87%), Query Frame = 0

Query: 68  PQTPDAARRGAET-DAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTKDIRLFI 127
           P   + + RG    D    LLRERIVFLG  +DD VAD+I++QLL L+A+D  KDI+L+I
Sbjct: 39  PTVVEQSGRGERAFDIYSRLLRERIVFLGTGVDDAVADSIVAQLLFLEAEDPEKDIQLYI 98

Query: 128 NSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNARIMVHQ 187
           NS GGS++A MAIYD +Q V  DV+TI  G+AAS  + +L GG +GKR+A+P+ARIM+HQ
Sbjct: 99  NSPGGSVTAGMAIYDTMQQVAPDVATICFGLAASMGAFLLSGGAQGKRMALPSARIMIHQ 158

Query: 188 PLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPIEAVEYG 247
           PLGGA GQA+D+EIQAREI+++K+ +  ++++ TG P EK++ D DRD +MSP EA  YG
Sbjct: 159 PLGGAQGQAVDIEIQAREILYHKSTLNDLLAQHTGQPLEKIEVDTDRDFFMSPEEAKAYG 218

Query: 248 LIDGVIDKDSI 258
           LID V+ + ++
Sbjct: 219 LIDQVLTRPTM 229

BLAST of Lsi07G011300 vs. ExPASy TrEMBL
Match: A0A1S3BJF4 (ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo OX=3656 GN=LOC103490719 PE=3 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 6.0e-150
Identity = 284/294 (96.60%), Postives = 290/294 (98.64%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLGLPNSHGKPSNFFPKLKSTLSFSSASSVLKTTSLKPSRTLAPP 60
           MELLSRCSTLIP ASSLGLPNSHGKPSNFFPKLKSTLSF SASSVLKTT+LKPSRTL PP
Sbjct: 1   MELLSRCSTLIPQASSLGLPNSHGKPSNFFPKLKSTLSFPSASSVLKTTALKPSRTLPPP 60

Query: 61  CSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSV MTAPQTPDA+RRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  CSV-MTAPQTPDASRRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 295
           EAVEYGLIDGVID+DSIIPL+PVPERVKA+LNYEE+SKDPRKFLTPDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDRDSIIPLVPVPERVKATLNYEEMSKDPRKFLTPDVPDDEIY 293

BLAST of Lsi07G011300 vs. ExPASy TrEMBL
Match: A0A6J1CWB9 (ATP-dependent Clp protease proteolytic subunit OS=Momordica charantia OX=3673 GN=LOC111014827 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 8.7e-149
Identity = 281/294 (95.58%), Postives = 285/294 (96.94%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLGLPNSHGKPSNFFPKLKSTLSFSSASSVLKTTSLKPSRTLAPP 60
           MELLSRCSTLIPHASSL LPNSH KPSNF+PKLKS+LSF SA SVLK T+LKPSRTLA P
Sbjct: 1   MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP 60

Query: 61  CSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSVLMTAPQTPD ARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINS GGSLSATMAIYDVVQLVRADVSTI LGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPF+KVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 295
           EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 294

BLAST of Lsi07G011300 vs. ExPASy TrEMBL
Match: A0A6J1EC18 (ATP-dependent Clp protease proteolytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111431188 PE=3 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 3.1e-146
Identity = 277/294 (94.22%), Postives = 286/294 (97.28%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLGLPNSHGKPSNFFPKLKSTLSFSSASSVLKTTSLKPSRTLAPP 60
           MELLSRCSTLIPHASSLGL NSHGKPSNF+PKLKS+LSF SASSVLKTT+ KPSRTLA  
Sbjct: 48  MELLSRCSTLIPHASSLGLLNSHGKPSNFYPKLKSSLSFPSASSVLKTTARKPSRTLATS 107

Query: 61  CSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSV MT PQTPDAA+RGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 108 CSVHMTGPQTPDAAQRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 167

Query: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINS+GGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 168 DIRLFINSSGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 227

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNN+TRIISEFTGHPFEKVQKDIDRDRYMSPI
Sbjct: 228 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNITRIISEFTGHPFEKVQKDIDRDRYMSPI 287

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 295
           EAVEYGLIDGVIDKDSIIPL+PVPERVKASLNY EISKDP+KFL+PDVPDDEIY
Sbjct: 288 EAVEYGLIDGVIDKDSIIPLVPVPERVKASLNYIEISKDPKKFLSPDVPDDEIY 341

BLAST of Lsi07G011300 vs. ExPASy TrEMBL
Match: A0A6J1HNH6 (ATP-dependent Clp protease proteolytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111465249 PE=3 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 3.8e-144
Identity = 274/294 (93.20%), Postives = 283/294 (96.26%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLGLPNSHGKPSNFFPKLKSTLSFSSASSVLKTTSLKPSRTLAPP 60
           MELLSRCSTLIPHASSLGL NSHGKPSNF+PKLKS LSF SASSVLKTT+ KPSRTLA  
Sbjct: 1   MELLSRCSTLIPHASSLGLLNSHGKPSNFYPKLKSGLSFPSASSVLKTTARKPSRTLATS 60

Query: 61  CSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
            SV MT PQTPDAA+RGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  SSVHMTGPQTPDAAQRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINS+GGSLSATMAIYDV+QLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSSGGSLSATMAIYDVIQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNN+TRIISEFTGHPFEKVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNITRIISEFTGHPFEKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 295
           EAVEYGLIDGVIDKDSIIPL+PVPERVKASLNY EISKDP+KF +PDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDKDSIIPLVPVPERVKASLNYIEISKDPKKFFSPDVPDDEIY 294

BLAST of Lsi07G011300 vs. ExPASy TrEMBL
Match: A0A0A0L3S1 (ATP-dependent Clp protease proteolytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3G005040 PE=3 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.6e-142
Identity = 270/294 (91.84%), Postives = 280/294 (95.24%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLGLPNSHGKPSNFFPKLKSTLSFSSASSVLKTTSLKPSRTLAPP 60
           MELLSRCSTL PHASSLGLPNSHGKPSNFFPKLKSTLSF SASSVLKTT+LKPSRTL PP
Sbjct: 1   MELLSRCSTLTPHASSLGLPNSHGKPSNFFPKLKSTLSFPSASSVLKTTALKPSRTLPPP 60

Query: 61  CSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSV MTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDA+DSTK
Sbjct: 61  CSV-MTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAKDSTK 120

Query: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINSAGGSLS+TMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSAGGSLSSTMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASG A+DVEIQAREIM NK+NV RIISEFTGHPFEKVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGLALDVEIQAREIMQNKDNVIRIISEFTGHPFEKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 295
           EAVEYG IDGVID+DSIIPLMPVP++VK   NY E+ KDP KFLTPDVPDDEI+
Sbjct: 241 EAVEYGFIDGVIDQDSIIPLMPVPDKVKGKFNYTEVMKDPMKFLTPDVPDDEIF 293

BLAST of Lsi07G011300 vs. NCBI nr
Match: XP_038876611.1 (ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Benincasa hispida])

HSP 1 Score: 541.6 bits (1394), Expect = 4.3e-150
Identity = 284/294 (96.60%), Postives = 287/294 (97.62%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLGLPNSHGKPSNFFPKLKSTLSFSSASSVLKTTSLKPSRTLAPP 60
           MELLSRCSTL+PHASS+GLP SHGKPSNF PKLKSTLSF SASSVLKTT+ KPSRT APP
Sbjct: 1   MELLSRCSTLLPHASSIGLPTSHGKPSNFLPKLKSTLSFPSASSVLKTTAPKPSRTPAPP 60

Query: 61  CSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  CSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 295
           EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDP KFL PDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPTKFLNPDVPDDEIY 294

BLAST of Lsi07G011300 vs. NCBI nr
Match: XP_008448589.1 (PREDICTED: ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Cucumis melo])

HSP 1 Score: 540.0 bits (1390), Expect = 1.2e-149
Identity = 284/294 (96.60%), Postives = 290/294 (98.64%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLGLPNSHGKPSNFFPKLKSTLSFSSASSVLKTTSLKPSRTLAPP 60
           MELLSRCSTLIP ASSLGLPNSHGKPSNFFPKLKSTLSF SASSVLKTT+LKPSRTL PP
Sbjct: 1   MELLSRCSTLIPQASSLGLPNSHGKPSNFFPKLKSTLSFPSASSVLKTTALKPSRTLPPP 60

Query: 61  CSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSV MTAPQTPDA+RRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  CSV-MTAPQTPDASRRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 295
           EAVEYGLIDGVID+DSIIPL+PVPERVKA+LNYEE+SKDPRKFLTPDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDRDSIIPLVPVPERVKATLNYEEMSKDPRKFLTPDVPDDEIY 293

BLAST of Lsi07G011300 vs. NCBI nr
Match: XP_022145352.1 (ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Momordica charantia])

HSP 1 Score: 536.2 bits (1380), Expect = 1.8e-148
Identity = 281/294 (95.58%), Postives = 285/294 (96.94%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLGLPNSHGKPSNFFPKLKSTLSFSSASSVLKTTSLKPSRTLAPP 60
           MELLSRCSTLIPHASSL LPNSH KPSNF+PKLKS+LSF SA SVLK T+LKPSRTLA P
Sbjct: 1   MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP 60

Query: 61  CSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSVLMTAPQTPD ARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINS GGSLSATMAIYDVVQLVRADVSTI LGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPF+KVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 295
           EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 294

BLAST of Lsi07G011300 vs. NCBI nr
Match: KAG7015794.1 (ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 530.8 bits (1366), Expect = 7.5e-147
Identity = 278/294 (94.56%), Postives = 287/294 (97.62%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLGLPNSHGKPSNFFPKLKSTLSFSSASSVLKTTSLKPSRTLAPP 60
           MELLSRCSTLIPHASSLGL NSHGKPSNF+PKLKS+LSF SASSVLKTT+ KPSRTLAP 
Sbjct: 1   MELLSRCSTLIPHASSLGLLNSHGKPSNFYPKLKSSLSFPSASSVLKTTARKPSRTLAPS 60

Query: 61  CSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSV MT PQTPDAA+RGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  CSVHMTGPQTPDAAQRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINS+GGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSSGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNN+TRIISEFTGHPFEKVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNITRIISEFTGHPFEKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 295
           EAVEYGLIDGVIDKDSIIPL+PVPERVKASLNY EISKDP+KFL+PDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDKDSIIPLVPVPERVKASLNYIEISKDPKKFLSPDVPDDEIY 294

BLAST of Lsi07G011300 vs. NCBI nr
Match: XP_023553095.1 (ATP-dependent Clp protease proteolytic subunit 4, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 528.1 bits (1359), Expect = 4.9e-146
Identity = 277/294 (94.22%), Postives = 286/294 (97.28%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLGLPNSHGKPSNFFPKLKSTLSFSSASSVLKTTSLKPSRTLAPP 60
           MELLSRCSTLIPHASSLGL NSHGKPSNF PKLKS++SF SASSVLKTT+ KPSRTLAP 
Sbjct: 1   MELLSRCSTLIPHASSLGLLNSHGKPSNFHPKLKSSVSFPSASSVLKTTARKPSRTLAPS 60

Query: 61  CSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSV MT PQTPDAA+RGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  CSVHMTGPQTPDAAQRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINS+GGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSSGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNN+TRIISEFTGHPFEKVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNITRIISEFTGHPFEKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 295
           EAVEYGLIDGVIDKDSIIPL+PVPERVKASLNY EISKDP+KFL+PDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDKDSIIPLVPVPERVKASLNYIEISKDPKKFLSPDVPDDEIY 294

BLAST of Lsi07G011300 vs. TAIR 10
Match: AT5G45390.1 (CLP protease P4 )

HSP 1 Score: 371.3 bits (952), Expect = 7.1e-103
Identity = 212/299 (70.90%), Postives = 234/299 (78.26%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLGLPNSHGKPSNFFPK-----LKSTLSFSSASSVLKTTSLKPSR 60
           M  LS  S+L P   S  L +S    S+ FPK     LK T      S  L+TTS  P R
Sbjct: 1   MGTLSLSSSLKPSLVSSRLNSSSSASSSSFPKPNNLYLKPT---KLISPPLRTTSPSPLR 60

Query: 61  TLAPPCSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDA 120
                 +  +   QT ++A RGAE+D MGLLLRERIVFLG+SIDDFVADAI+SQLLLLDA
Sbjct: 61  F----ANASIEMSQTQESAIRGAESDVMGLLLRERIVFLGSSIDDFVADAIMSQLLLLDA 120

Query: 121 QDSTKDIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRL 180
           +D  KDI+LFINS GGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILG GTKGKR 
Sbjct: 121 KDPKKDIKLFINSPGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGAGTKGKRF 180

Query: 181 AMPNARIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDR 240
           AMPN RIM+HQPLGGASGQAIDVEIQA+E+MHNKNNVT II+  T   FE+V KDIDRDR
Sbjct: 181 AMPNTRIMIHQPLGGASGQAIDVEIQAKEVMHNKNNVTSIIAGCTSRSFEQVLKDIDRDR 240

Query: 241 YMSPIEAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 295
           YMSPIEAVEYGLIDGVID DSIIPL PVP+RVK  +NYEEISKDP KFLTP++PDDEIY
Sbjct: 241 YMSPIEAVEYGLIDGVIDGDSIIPLEPVPDRVKPRVNYEEISKDPMKFLTPEIPDDEIY 292

BLAST of Lsi07G011300 vs. TAIR 10
Match: AT1G66670.1 (CLP protease proteolytic subunit 3 )

HSP 1 Score: 196.4 bits (498), Expect = 3.1e-50
Identity = 110/240 (45.83%), Postives = 153/240 (63.75%), Query Frame = 0

Query: 24  GKPSNF------FPKLKSTLSFSSASSVLKTTSLKPSRTLAPPCSV----LMTAPQTPDA 83
           GK  NF       PK        S+ S+ K     P +TL+    V    + +  Q+P  
Sbjct: 22  GKNLNFPIRNHRIPKTSKPFCVRSSMSLSK----PPRQTLSSNWDVSSFSIDSVAQSPSR 81

Query: 84  ARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTKDIRLFINSAGGSL 143
                E D   +LLR+RIVFLG+ +DD  AD +ISQLLLLDA+DS +DI LFINS GGS+
Sbjct: 82  LPSFEELDTTNMLLRQRIVFLGSQVDDMTADLVISQLLLLDAEDSERDITLFINSPGGSI 141

Query: 144 SATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNARIMVHQPLGGASG 203
           +A M IYD ++  +ADVST+ LG+AAS  + +L  G+KGKR  MPN+++M+HQPLG A G
Sbjct: 142 TAGMGIYDAMKQCKADVSTVCLGLAASMGAFLLASGSKGKRYCMPNSKVMIHQPLGTAGG 201

Query: 204 QAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPIEAVEYGLIDGVID 254
           +A ++ I+ RE+M++K  + +I S  TG P  +++ D DRD +++P EA EYGLID VID
Sbjct: 202 KATEMSIRIREMMYHKIKLNKIFSRITGKPESEIESDTDRDNFLNPWEAKEYGLIDAVID 257

BLAST of Lsi07G011300 vs. TAIR 10
Match: AT1G02560.1 (nuclear encoded CLP protease 5 )

HSP 1 Score: 179.1 bits (453), Expect = 5.2e-45
Identity = 87/167 (52.10%), Postives = 124/167 (74.25%), Query Frame = 0

Query: 86  LLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTKDIRLFINSAGGSLSATMAIYDVVQL 145
           L + RI+  G ++DD +A+ I++QLL LDA D TKDI +++NS GGS++A MAI+D ++ 
Sbjct: 119 LFQYRIIRCGGAVDDDMANIIVAQLLYLDAVDPTKDIVMYVNSPGGSVTAGMAIFDTMRH 178

Query: 146 VRADVSTIALGIAASTASIILGGGTKGKRLAMPNARIMVHQPLGGASGQAIDVEIQAREI 205
           +R DVST+ +G+AAS  + +L  GTKGKR ++PN+RIM+HQPLGGA G   D++IQA E+
Sbjct: 179 IRPDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEM 238

Query: 206 MHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPIEAVEYGLIDGVI 253
           +H+K N+   ++  TG   EK+ +D DRD +MS  EA EYGLIDGVI
Sbjct: 239 LHHKANLNGYLAYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVI 285

BLAST of Lsi07G011300 vs. TAIR 10
Match: AT1G11750.1 (CLP protease proteolytic subunit 6 )

HSP 1 Score: 158.7 bits (400), Expect = 7.2e-39
Identity = 78/188 (41.49%), Postives = 115/188 (61.17%), Query Frame = 0

Query: 68  PQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTKDIRLFIN 127
           P  P     G   D   +L R RI+F+G  I+  VA  +ISQL+ L + D   DI +++N
Sbjct: 83  PVMPSVMTPGGPLDLSSVLFRNRIIFIGQPINAQVAQRVISQLVTLASIDDKSDILMYLN 142

Query: 128 SAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNARIMVHQP 187
             GGS  + +AIYD +  ++  V T+A G+AAS  +++L GG KG R AMPN R+M+HQP
Sbjct: 143 CPGGSTYSVLAIYDCMSWIKPKVGTVAFGVAASQGALLLAGGEKGMRYAMPNTRVMIHQP 202

Query: 188 LGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPIEAVEYGL 247
             G  G   DV  Q  E +  +  + R+ + FTG P EKVQ+  +RDR++S  EA+E+GL
Sbjct: 203 QTGCGGHVEDVRRQVNEAIEARQKIDRMYAAFTGQPLEKVQQYTERDRFLSASEALEFGL 262

Query: 248 IDGVIDKD 256
           IDG+++ +
Sbjct: 263 IDGLLETE 270

BLAST of Lsi07G011300 vs. TAIR 10
Match: AT1G11750.2 (CLP protease proteolytic subunit 6 )

HSP 1 Score: 157.1 bits (396), Expect = 2.1e-38
Identity = 89/245 (36.33%), Postives = 136/245 (55.51%), Query Frame = 0

Query: 27  SNFFPKLKSTLSFSSASSVLKTTSLKPSRTLAPPCSVLMTA----------------PQT 86
           S +   LK+ LS + + S +K  +  PS    P  ++L ++                P  
Sbjct: 44  SPYGDSLKAGLSSNVSGSPIKIDNKAPSSLPLPILNILKSSTVYFIFGVIEAKKGNPPVM 103

Query: 87  PDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTKDIRLFINSAG 146
           P     G   D   +L R RI+F+G  I+  VA  +ISQL+ L + D   DI +++N  G
Sbjct: 104 PSVMTPGGPLDLSSVLFRNRIIFIGQPINAQVAQRVISQLVTLASIDDKSDILMYLNCPG 163

Query: 147 GSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNARIMVHQPLGG 206
           GS  + +AIYD +  ++  V T+A G+AAS  +++L GG KG R AMPN R+M+HQP  G
Sbjct: 164 GSTYSVLAIYDCMSWIKPKVGTVAFGVAASQGALLLAGGEKGMRYAMPNTRVMIHQPQTG 223

Query: 207 ASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPIEAVEYGLIDG 256
             G   DV  Q  E +  +  + R+ + FTG P EKVQ+  +RDR++S  EA+E+GLIDG
Sbjct: 224 CGGHVEDVRRQVNEAIEARQKIDRMYAAFTGQPLEKVQQYTERDRFLSASEALEFGLIDG 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94B601.0e-10170.90ATP-dependent Clp protease proteolytic subunit 4, chloroplastic OS=Arabidopsis t... [more]
Q3ANI87.8e-5454.36ATP-dependent Clp protease proteolytic subunit 1 OS=Synechococcus sp. (strain CC... [more]
Q3B0U11.7e-5353.85ATP-dependent Clp protease proteolytic subunit 2 OS=Synechococcus sp. (strain CC... [more]
Q7V9921.1e-5255.08ATP-dependent Clp protease proteolytic subunit 1 OS=Prochlorococcus marinus (str... [more]
O341252.5e-5253.93ATP-dependent Clp protease proteolytic subunit 2 OS=Synechococcus elongatus (str... [more]
Match NameE-valueIdentityDescription
A0A1S3BJF46.0e-15096.60ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1CWB98.7e-14995.58ATP-dependent Clp protease proteolytic subunit OS=Momordica charantia OX=3673 GN... [more]
A0A6J1EC183.1e-14694.22ATP-dependent Clp protease proteolytic subunit OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HNH63.8e-14493.20ATP-dependent Clp protease proteolytic subunit OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A0A0L3S11.6e-14291.84ATP-dependent Clp protease proteolytic subunit OS=Cucumis sativus OX=3659 GN=Csa... [more]
Match NameE-valueIdentityDescription
XP_038876611.14.3e-15096.60ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Benincasa hispi... [more]
XP_008448589.11.2e-14996.60PREDICTED: ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Cucu... [more]
XP_022145352.11.8e-14895.58ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Momordica chara... [more]
KAG7015794.17.5e-14794.56ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Cucurbita argyr... [more]
XP_023553095.14.9e-14694.22ATP-dependent Clp protease proteolytic subunit 4, chloroplastic-like [Cucurbita ... [more]
Match NameE-valueIdentityDescription
AT5G45390.17.1e-10370.90CLP protease P4 [more]
AT1G66670.13.1e-5045.83CLP protease proteolytic subunit 3 [more]
AT1G02560.15.2e-4552.10nuclear encoded CLP protease 5 [more]
AT1G11750.17.2e-3941.49CLP protease proteolytic subunit 6 [more]
AT1G11750.22.1e-3836.33CLP protease proteolytic subunit 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001907ATP-dependent Clp protease proteolytic subunitPRINTSPR00127CLPPROTEASEPcoord: 152..169
score: 50.56
coord: 173..192
score: 57.75
coord: 121..141
score: 54.88
coord: 81..96
score: 46.88
coord: 230..249
score: 56.38
IPR001907ATP-dependent Clp protease proteolytic subunitHAMAPMF_00444ClpPcoord: 66..255
score: 36.73835
IPR001907ATP-dependent Clp protease proteolytic subunitCDDcd07017S14_ClpP_2coord: 81..251
e-value: 2.04695E-96
score: 279.326
NoneNo IPR availableGENE3D3.90.226.10coord: 62..257
e-value: 7.2E-69
score: 233.5
NoneNo IPR availablePANTHERPTHR10381:SF24ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT 4, CHLOROPLASTICcoord: 62..291
IPR023562Clp protease proteolytic subunit /Translocation-enhancing protein TepAPFAMPF00574CLP_proteasecoord: 80..254
e-value: 1.7E-71
score: 239.9
IPR023562Clp protease proteolytic subunit /Translocation-enhancing protein TepAPANTHERPTHR10381ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNITcoord: 62..291
IPR033135ClpP, histidine active sitePROSITEPS00382CLP_PROTEASE_HIScoord: 174..187
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 80..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G011300.1Lsi07G011300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006515 protein quality control for misfolded or incompletely synthesized proteins
biological_process GO:0006508 proteolysis
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0009368 endopeptidase Clp complex
cellular_component GO:0009526 plastid envelope
molecular_function GO:0051117 ATPase binding
molecular_function GO:0004176 ATP-dependent peptidase activity
molecular_function GO:0004252 serine-type endopeptidase activity