Lsi07G010040 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G010040
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description1,4-alpha-D-glucan glucanohydrolase
Locationchr07: 14339603 .. 14349458 (-)
RNA-Seq ExpressionLsi07G010040
SyntenyLsi07G010040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAGCTTCCAACAAAATTAATTCAGCTTTTTGGGCATATAAAAGGCATTTCCTCCCCGCCATTCCACCTCCCTTTTCTTCGTCATTACATTTTTAACTTCCGGCTCTTTCGAACAACACATCTCAAGAATGCAGATCCTGCGCTCCTATTGATTTTGCAATTCAATTCAGGTTTTCAAGCTTTTCAAATATTTCTATGTTCCGTTTTTTGTTCTACATCTTCCAATCACCCTTGATTCTGATCAGATTACTCACTTTTCACTCCCATTTTCACTTCCTTGTTTCGTTTTGATCTTTTTGTCTTTCTTAATGTTGGTGGCTTTTCAGCCTCTCGTGAATTTCTCCTTTCTACTGTACCATTTTGTGCTTGGGTAATAATTCGATCCAGAGAATAGTTAGGCATAAATTATGTATCTCGTGGTTGAATTTGATTCATTTGAAATGTAATTTGGTTTCACTCGTAAAATTGAGTTTTCCCTGCAGGAAGTAATGGGTCCATTCCCATTGCTTGATACAGCAATTGAAATCTTTCCGCGTTGCCCTATTATCACTTCGGGTTCTTCATATGGCAGGCGCTCATCAAATTGCCATCACATTCTAAGAACAGTTTCAGCTACCAGGAACTGGTATTTGTGCTTTTACTCTGCCTGCATGTCTTCTACCCTGGAAACAGGGGGATGAGATTTTGGTACCCTACCATGATTTTTGCCAGTCCTTTTTCTGTTTTCGTTTCTATTCGATTACGATCAAATTGGTAATGTTAATGCAGGAAAGTTTCATACATTGAAAACTTGCAGTCTAAACGAAACACTGTTGCCTTTTCTAGTCGGGTATGGATATTTTTTGAACCTACATTCTTTATTGTTTTCATTATTTAAATCTGCCCGCTAGCTGCGATGATTGAACAGAGTTTTACGAATCATCATATCAGTTCTTATTTAAATGTTTTTTCAAATTACTCTAGTAACATGGAAATGCTAGGCGAGATCATTTCCTGGAATGGAGATTGGGATTTAACTCATGTATTCAACTACAACTTTATGTACAAGTGGAGAAAGTCTGACGATATTTATGGCTGTTGGATTTGTAACAGGACAATTCAGGTGATCATTTAACAGATTTGGTAGATGATGCTGATGGCTTTTCAATTGGTAGAAGTGAAGTATTGGAGACAGAAGAAGATGAAATTCTTGCAGTTAAAAAAGCGCTTTTGGAGTCTCAAACAAGACAAGAAGCTGTAGAGAAAGAGAGAGATCAACTGCTTGAGAGGTTAGCTCGCTATGAGGCTAAACAAAAAGAATATGTTGCTACTATATTGCATGATAAGGAATTGGCAGTTTCAGAACTCGAAGCTGCCAGATCATTATTTAATAAGAAACTTGAGGAATCAGTAGGTGAGAAGTTTGCTTTGGAGTCTAAGCTGGTCCTTGCAAAGCAGGATGCTATTGATCTTGCAGTGCAGGTTGAAAAGTTAGCCGCAATTGCCTTCCAGCAGGCCACTTCACACATATTAGAAGATGCTCAATACAGAGTATCAGTTGCAGAAACTTCTGCTGTTGAGACGTCTTATGATATTGAAAAACAGATCAGGGATGCTACTGAAGGTTCAATGCTGTCATTTGTAGAACAATCAAAAATAGCTATTGAGAAGGCACTGGATGTGGCTGAGAAAGCTAGTGCTCATGCAAAGAAGGCTGTGGCTACATTTACTGATGAGGTATATCCTTTAGATGAGATTGCCTCTATCCAATCAGAAAATATTAAGTTGAAAGGTGTCGTCAATGAAATGGAATCTCACTTATCACTTGCAAGAACTGATATTGATAACCTCAAGTTGGAACTGGAGCAAGCCCGAGCACAAGCAACTGCATCAGAAATTCGAGCCAAAAATGCTGAGAAAGCATTGGTCGAATTCCAGGAGTTGAGCAGGGAAAAGATCATTCAACAAGAGGGAGAAATTAAATTGATGATGGAGAAAATCAAGAAAGATGTAGCAGACAAGAAGAAAGCTGCTTCCAAAGCTTTCAAGGCTGAGCTTGAAGGTATTAAGTCTGCCATTCATGCTGCCAAAGAAACTGCACATTCCAAAGATAACGCCTATATGAGAAGATGTGAAGCACTGCAAAGATTATTGAGGGCGTCGGAAGCTGCAACAAAGATGTGGCAACAACGAGCGGATATGGCGGAGTCATTTTTATTGAAGGAAAGAACCATGGGTAAAGATAATGAAGTTGCAGCTTATATTGTCAATGGTGGGCGGATAGACCTCTTGACAGATGATGAGTCACAAAAGTGGAAACTCTTGAGCGAAGGACCTCGAAGAGAGATACCTCAATGGATGGCTAGAAGAATTGGAACCATTCGTCCCAAGTTTCCTCCTAGAAAGATTGATTTAACTGAAATTTCAACATCAAAGTTTAGATCATTGGACTTGCCCAAACTTGAAGAAGTATGGTCAATTGCTCAAGAAAAGCCAAAAATAGGGGATACACTAATTGAGCATGTTATCGAGAAAGAAACAATAGAAAAGAAAAGAAAGGCTCTTGAGCGTGCACTGCAACGAAAGACCATACAATGGCAGAGGACCCCTGATCAAACAAAATTGGGTCAGCTGGCTTTATCATTTTTCCTATTAATGTAATCAAAAAAAGATTTAGTGGTATAAACCTAACATTCAATTCTTGTGGACTCTGATACAGAACCAGGAACTGGGACTGGACACGAAATTGTGGTTAGTTATCACCAATGACATCAGCAAACGGATGCATTTTACATGGATTTACATAATTAAATTCTTAGTAGTTCAAACTTGATAATTTAGTTTGGAAAGAATAAGTTTATTGTTTCAAAATATTTTTTACTTGACCTTTATTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCATGTTTCATAGTTGCATCACTCTAGATTTACTCAAGTTATAATGTAAATCACCTTTTTGATTGATTTTGTTGTAGTTGATCTCTTGTTTTTTTAGTAAGGGATAGGTATCACACCATACTAAAGTGTCAAGGGTTTAACATGTCTTGGGCTGTACTTAGGCTAAGTGGCTAAACAATGCTAGCTCTAGTGGAGGTGTTTGACAAGTTAGCTGACCATGTTAATGTGGACGATAAACATGGGGAATAGTTGTGGGCAAAGTTGAGTGTGCTAGGAGCGCAGCTGGAGCTGCGTAGACAACCAACGTCAGGTTGAAAACAACGGGGATGGTGTGTTGTACATAGATGCAATTGCCATGTGTTAAACATATAGAGACCCTTCTAAGAGTTTGTTAATCTTCCGTATGAGACTTTGAATATAAATAATGTGAAGTTCAGAATCTTCTAGGAGTTGTTTTCTCAGTGATTTATTGGTAGACCTCTCCCATTGAGAACCCATTATTTTCTCAGTTATAGAATATTCATGTTATTATCTATCTGTTCCAACCTTGACTGATGATTGTTAATGGATACCTCTTGAAGTTTCAAGGTTTCAACTGGGAAAGCTGGAGAAGACGCTGGTACCTGGAGTTAGCTGCCAAAGCATCTGATTTATCTCAGTCTGGGATAACAGCAGTGTGGCTCCCTCCACCAACAGAATCTGTTGCTCCACAAGGTTTGCTCCATGTCATTTTTCTTTTATATTGTTTAAGGAAAATAAAATACCAATAAGCTCTTCAAATCTTTCATACGCTTCTAAAACTTTTTTTCCCAATTTGGTTAACATGGGATAGTTTCAATTTCGATGTCTGGGCTAAAATTTACAAACAGTGGGAAATATTTTGCAGGTTATATGCCATCTGACCTATATAATTTGAACTCATCTTATGGATCTGAGGAAGAACTCAAGTTCTGTATAGAAGAATTTCATTCTCAAGATCTTCTGGTATGTCATGGTCTGAAACTTGTCTGATGTGTTTCATATTTATTTAATTCCACTATTTGTTTCAATTATTCTTGGAACGGGAAGAAATTGAATATATTCAAGCAAGTGCTCATCTGTAGAATTTTATATGTTAAACTGTTTCCATTAGAGATAGGTGCTAGAAACTCTTAGAAAAATACACTACTTAATTTTCAAAATATCATTGAAAAGGCAACTTAGCAGAAGATACGTTTTTTTGTTTTTTAATCATTACTTTTTTATTTTTTTATTTTTTTTTATTGAAACAGACTTCAGCATCTAGAGTGCTGTGCCAAAATCTGTCGATGTAGAATCCTTACACACATGTTATTAACCTATACTAAGGAAATAAAAATAAACTATATCAGAAAGTTGCAAATTGGATGATATAGAACTCTGTTAGACCACCAATTGTGAACGCGTAATTCTAGGAAGGTGTGTAACTCCCACATGTTAATTATATGGTATGGTTTTGAGTGAGCTGTAACTCCATAGGTGATTACGTAAAAAGAATGGTCTTTGTCTTATTGTCTTTTTGTTAAGTGTTCAATTCCCCCCTTCTTTTTGACTAATATTATTTGAATGTGCCTCCAGGCCCTCGGTGATGTTGTACTCAATCATAGATGTGCACACAAGCAGGTCTACTCCTGTCTCACAGCATCCTATTTTTACTATTAGCTCATTTTCCCTTTTATGTTAATAAGATCAATTCTTTCTATATAAACGTTTCATGTAGCAATGGCATTACAACATTTCACAACTTATCTAATAATTGAACTTGCATGATAATTGTTTGTTATTTCAACTTCTTTCATAAATTTATGTTGTAAAATAAAGTTGATGTTGAGTACGTTAAGAATAGAAGAATTGTACGTGGTTTGTTCTCTGGAACTTGCTCTGCTGGTAGCAACTTTATTGTAAACAGTATTGAAAAATTGATATTTCTTGATTGCATGCGTCTTTTCAATGGATAGGACTGACCTTGTTATTAGTTTCTTTTGGAAGTTCAGAGATATGTTGTTTACCTTTATGACTTCAAAGGCTGTGTTGAATGACCCTTATATCATGTATCTGCTGTCTATGACTGATTAAAGCAGGTAGAATTTTGCAGGGATGCTAACAGACCTTTTGGTGCGCCTCAGAGTAGTTGTACCTTTATATAACTTAGTGCTCTTGCTGGTTTCAGAGTCCCAATGGGGTTTGGAACATTTTCGGTGGTAAGCTTACTTGGGGACCTGAAGCAATTGTTTGTGATGATCCGAATTTTCAGGGTCGAGGAAATCCTTCAAGTGGTAATTCTTAGCTTTTAGCTACTGATTGAACTTTGAATGCTATTATCTGAGATTTTGTGTGGATATCTAGTCTGTCATCTTGACAGTGCTACATTGACATGAAAGCAAGTTGTTCCTTTAGTTGGCATGACAAATGCAGTGCTTTAATTTATTAATTCTTTACCTTGTAATTGTTTTTAGCTCTTGATAATTTCAAGTTTAATACGGTTGCTTTATACAGCATAAGTTTGCTATCACATAAAAATTAGAATGAGCAGATATGTTGCTCACTCATGAAAAGCTTTGGTTTGGTTATCAATGCTACTTTTTGTTTGTTTGTGATGGCTGTAGGCGACATCTTCCATGCGGCACCAAATATTGATCATTCGCAGGACTTTGTGAGGAGAGACATAAAAGAATGGCTAAATTGGCTTCGCAATGATATTGGTTTTGATGGGTGGCGCCTTGATTTTGTGAGGTAATTTCTCTTCGATTATTTTCTTCAGTATAGATGGTTTCTCATAAACAGTAAATAAATACTAAATTGAAAAAAATATTCTCTGTTCATTATATTATCCCCATCATGAAGTTTCCTTCACACAGAACCACATAGGATTAAGATGATCAAGAATGAAGCTCATACCGCTTCAAACTTTTAATTTTTTTCAGTCAGCTGGTATTAAAATCGGGATAATCTGGATCAAAATGTCATCATACTTCCAAAATTAGTTACACTTATGCACCTGGATATTGATTATTATTATTTTTTTTTTTTTTTTTTTTTTGGTATTATAGGGGTTTCTCGGGTACATATGTTAAAGAATATATTGAGACTTCAAATCCTGCTTTTGCCATCGGAGAATACTGGGACAGTTTGGCTTATGAACATGGGAATTTATGTTATAACCAAGGTAATAAGTTGCTGTACATTCTTGGACTAATCTTCATGAACAATTTTATTCTTAGTCTCTGATGACTTACTTGCGTATATGAGATTCTCTGGTTCCTTTATGTAATACAAATTTAAGAAAATAGTGGATACAAATGTTTTATGGTTGGCACTGTGCCCATGTTTATTTTATATGTATACTGTAGGACGTGGGGATTTTAAGTGGATGTAATCCTGCGCTAAAAACTTTCCCTTCATGTTTCTATGATCACTTACCTCACACTATGCTAATTATTTTCAACTTGCTCGTATATTTAGATGCTCATCGGCAACGAATAGTTGATTGGATCAATGCCACAGGTGGCACTTCCTCTGCATTCGATGTTACCACCAAGGTATTCTTTGTTTTTATGTTCCATTCCTGATTTTGAAGAAAGTAAAGGAAAAAACTGGTGGACGACATGGGCCCAAAAAATGTCCAGGTGCCACCTTCTTTAGAGTACTAGGTGGCCTATTAGAAAAAATGTGGAGTTTTATTATTATTATTATTATTATTCTTTTCTGGATATGGTACTGTGCTATATATTTTGGAGTGAAGGAATCATGCAGTGAGTAAAACCATTTGTAATAGATTCTAGCTAAATTTTAAAATTTATACTTTGTCAAATATGTTCTTATACACAAGTGTGTTAAGAGTATTAATTGGTAAATATAGAGTTTTAATAGGATATGAATGCCATAGCGAGATAGCTGTTGTTAATTATGGGAGAAAGGTGACTATCTCAAAAGGCCATTACTGATGTAATTTCTCCTCGATATTGTACAATATATTTTCAATGTTGTTTTCTTATAATAGGGATCCTACCAAAGTGTTTTCTTTGTTTGATAATCTTGACATCTAAGTAATCGCTCCCACTCAGGGGATATTGCATTCTGCACTTCATAACCAATATTGGAGGTTGATAGACCCACAAGGAAAACCAACAGGAGTAGTGGGATGGTGGCCTTCCCGTGCTGTCACTTTCTTGGAAAACCATGACACTGGTTCCACACAGGTTTGAAACTAGCAGCGAGGCTGTCATTGCATTAGGTGACAGGTTTCACTTTCATGGACAATCATATATCATATACAAATGGCAGTAAAAATCTGATATTTTATACATCTGTGTCAAGTAGAACCGATGTGAAAAATTATTAACGAAAGCAACTGCTTAGAGAGAATCACATAGTTTGATGTAAACGGCTTCTCCAAGCTTGCATTTTGGTCCAGATTTTCAATTATCTTTTGTAGATTTATACCTTATTTGGTTTAGATACTATTCCTTTTATATTCACGCATGAGCTTTATTTATGTACTTAATATAGCTAGTTTCCTTTTTTGTTGTTTTCTATTGTATATCTTCCTGTAATTTAGAGTTTCAATAATACATTTTTGAGTAATTTTTCACCCAAAACTTCATCTTTAAGTGAAATTGAAATAAAGTATTATAGTTTGGTTCAATAGCCAAAGGGACCAACCATAAACCAAAGATCTTTGAAGTCATAAGTTCAATGCATGTTGTAGAGTCGGGCTGATTGTCCCGACTTAAATGAAGATGTGCATAAATTTCTTGGACACTCATGGTTATTAAACAAAAAATAAATAAATAATGTAAGAGAATATTTTTGGATAGATTCGTTTGTGAATTGAGCACCTCAACATAAATGTAAAAATCTCATTTTCCTTGAGACATTTCTTGGCTGCTATAGTATGAATTATTTTCATTCTGGAAGCACCTTAACATTAAAGCTAGCCTCCATTATTTGAATTTTTCCGTACTGAGATCTTGTTGATTTACATGCTAGTAATTTTGGGGTAGCAGAAATTGAATTTGTCCAGTTGTTTCTTTACAGGGACACTGGCCATTTCCACGTGATAAACTTGCTCAAGGATATGCATATATCTTGACTCATCCTGGAACGGTAAGTAAGAAGTCGCTGAATCCTAAGAATATATATATATATTATTAGTTCATTTGGTTATCATTTCATTAGATAAGCTTCCTATTGTAGACTATAACATGAAGAAATGAAGAAGAGGGGAGAAAATTTGGGGGCGAGAATTATTAAAACTAAAAAGAGATATAGGTCAGAGGGGTGTTAGACTGTTAGGAAGAGAGTAAGCGGGATACGGATACGATATGATATCATCAAGAAAAAGGTGGGAAGCTAAGTATTTCTACTGAACAACCATATTTCAATCTAAGTTACATCGGTATGAGTTTGTTATCCGTTGTTGTGAGTTGCAGTTTTTTTTTAAAGGTTATTTCTTGTTTTTAAAGCTTTATAATACTGTAATTTTAGTCTTCGCTCTTTCTTAAGTTTAATTTTGCTGGCTTTGTCTTTCATTTTGCAGCCTACAATTTTCTACGACCACTTTTATGATTTTGGGATTCGTGAAATAATCAATGAGTTAATCGAGGCTCGACAACGTGCTGAGATCCATTGTCGGAGCTCTGTTAAGATCTACCATGCAAATAATGAAGGATATGTTGCACAGGTTGGAGATACACTGGTCATGAAGCTTGGACATTTTGATTGGAATCCCTCGAAGGAAAATCATTTGGATGGGAATTGGCAGAAGTTTGTTGACAAAGGATCAGATTACCAATTGTGGCTGAGACAATAATAATCCTTCCTATTAAAAGGAGGGATCCATTGCCAATCCAAATTCTAAGCAATTCCCAGTTTAAAAGCTCCTGCTATTATAATGTGAGCTTATGTTCTTTTGCATCTTAAATTTATAATGTGTTTAGTAGTTCTTCAAATTGTACCTTGTAATTCTGGATGATCCTGTTACCAGCAATAATATTTATGATACTTCACAAAACTCGGAGATATATGACCTTAAAATTTAGGTTTAAATACCATTATGGTTCTTATA

mRNA sequence

CAAGCTTCCAACAAAATTAATTCAGCTTTTTGGGCATATAAAAGGCATTTCCTCCCCGCCATTCCACCTCCCTTTTCTTCGTCATTACATTTTTAACTTCCGGCTCTTTCGAACAACACATCTCAAGAATGCAGATCCTGCGCTCCTATTGATTTTGCAATTCAATTCAGGAAGTAATGGGTCCATTCCCATTGCTTGATACAGCAATTGAAATCTTTCCGCGTTGCCCTATTATCACTTCGGGTTCTTCATATGGCAGGCGCTCATCAAATTGCCATCACATTCTAAGAACAGTTTCAGCTACCAGGAACTGGAAAGTTTCATACATTGAAAACTTGCAGTCTAAACGAAACACTGTTGCCTTTTCTAGTCGGGACAATTCAGGTGATCATTTAACAGATTTGGTAGATGATGCTGATGGCTTTTCAATTGGTAGAAGTGAAGTATTGGAGACAGAAGAAGATGAAATTCTTGCAGTTAAAAAAGCGCTTTTGGAGTCTCAAACAAGACAAGAAGCTGTAGAGAAAGAGAGAGATCAACTGCTTGAGAGGTTAGCTCGCTATGAGGCTAAACAAAAAGAATATGTTGCTACTATATTGCATGATAAGGAATTGGCAGTTTCAGAACTCGAAGCTGCCAGATCATTATTTAATAAGAAACTTGAGGAATCAGTAGGTGAGAAGTTTGCTTTGGAGTCTAAGCTGGTCCTTGCAAAGCAGGATGCTATTGATCTTGCAGTGCAGGTTGAAAAGTTAGCCGCAATTGCCTTCCAGCAGGCCACTTCACACATATTAGAAGATGCTCAATACAGAGTATCAGTTGCAGAAACTTCTGCTGTTGAGACGTCTTATGATATTGAAAAACAGATCAGGGATGCTACTGAAGGTTCAATGCTGTCATTTGTAGAACAATCAAAAATAGCTATTGAGAAGGCACTGGATGTGGCTGAGAAAGCTAGTGCTCATGCAAAGAAGGCTGTGGCTACATTTACTGATGAGGTATATCCTTTAGATGAGATTGCCTCTATCCAATCAGAAAATATTAAGTTGAAAGGTGTCGTCAATGAAATGGAATCTCACTTATCACTTGCAAGAACTGATATTGATAACCTCAAGTTGGAACTGGAGCAAGCCCGAGCACAAGCAACTGCATCAGAAATTCGAGCCAAAAATGCTGAGAAAGCATTGGTCGAATTCCAGGAGTTGAGCAGGGAAAAGATCATTCAACAAGAGGGAGAAATTAAATTGATGATGGAGAAAATCAAGAAAGATGTAGCAGACAAGAAGAAAGCTGCTTCCAAAGCTTTCAAGGCTGAGCTTGAAGGTATTAAGTCTGCCATTCATGCTGCCAAAGAAACTGCACATTCCAAAGATAACGCCTATATGAGAAGATGTGAAGCACTGCAAAGATTATTGAGGGCGTCGGAAGCTGCAACAAAGATGTGGCAACAACGAGCGGATATGGCGGAGTCATTTTTATTGAAGGAAAGAACCATGGGTAAAGATAATGAAGTTGCAGCTTATATTGTCAATGGTGGGCGGATAGACCTCTTGACAGATGATGAGTCACAAAAGTGGAAACTCTTGAGCGAAGGACCTCGAAGAGAGATACCTCAATGGATGGCTAGAAGAATTGGAACCATTCGTCCCAAGTTTCCTCCTAGAAAGATTGATTTAACTGAAATTTCAACATCAAAGTTTAGATCATTGGACTTGCCCAAACTTGAAGAAGTATGGTCAATTGCTCAAGAAAAGCCAAAAATAGGGGATACACTAATTGAGCATGTTATCGAGAAAGAAACAATAGAAAAGAAAAGAAAGGCTCTTGAGCGTGCACTGCAACGAAAGACCATACAATGGCAGAGGACCCCTGATCAAACAAAATTGGAACCAGGAACTGGGACTGGACACGAAATTGTGTTTCAAGGTTTCAACTGGGAAAGCTGGAGAAGACGCTGGTACCTGGAGTTAGCTGCCAAAGCATCTGATTTATCTCAGTCTGGGATAACAGCAGTGTGGCTCCCTCCACCAACAGAATCTGTTGCTCCACAAGGTTATATGCCATCTGACCTATATAATTTGAACTCATCTTATGGATCTGAGGAAGAACTCAAGTTCTGTATAGAAGAATTTCATTCTCAAGATCTTCTGGCCCTCGGTGATGTTGTACTCAATCATAGATGTGCACACAAGCAGAGTCCCAATGGGGTTTGGAACATTTTCGGTGGTAAGCTTACTTGGGGACCTGAAGCAATTGTTTGTGATGATCCGAATTTTCAGGGTCGAGGAAATCCTTCAAGTGGCGACATCTTCCATGCGGCACCAAATATTGATCATTCGCAGGACTTTGTGAGGAGAGACATAAAAGAATGGCTAAATTGGCTTCGCAATGATATTGGTTTTGATGGGTGGCGCCTTGATTTTGTGAGGGGTTTCTCGGGTACATATGTTAAAGAATATATTGAGACTTCAAATCCTGCTTTTGCCATCGGAGAATACTGGGACAGTTTGGCTTATGAACATGGGAATTTATGTTATAACCAAGATGCTCATCGGCAACGAATAGTTGATTGGATCAATGCCACAGGTGGCACTTCCTCTGCATTCGATGTTACCACCAAGGGGATATTGCATTCTGCACTTCATAACCAATATTGGAGGTTGATAGACCCACAAGGAAAACCAACAGGAGTAGTGGGATGGTGGCCTTCCCGTGCTGTCACTTTCTTGGAAAACCATGACACTGGTTCCACACAGGGACACTGGCCATTTCCACGTGATAAACTTGCTCAAGGATATGCATATATCTTGACTCATCCTGGAACGCCTACAATTTTCTACGACCACTTTTATGATTTTGGGATTCGTGAAATAATCAATGAGTTAATCGAGGCTCGACAACGTGCTGAGATCCATTGTCGGAGCTCTGTTAAGATCTACCATGCAAATAATGAAGGATATGTTGCACAGGTTGGAGATACACTGGTCATGAAGCTTGGACATTTTGATTGGAATCCCTCGAAGGAAAATCATTTGGATGGGAATTGGCAGAAGTTTGTTGACAAAGGATCAGATTACCAATTGTGGCTGAGACAATAATAATCCTTCCTATTAAAAGGAGGGATCCATTGCCAATCCAAATTCTAAGCAATTCCCAGTTTAAAAGCTCCTGCTATTATAATGTGAGCTTATGTTCTTTTGCATCTTAAATTTATAATGTGTTTAGTAGTTCTTCAAATTGTACCTTGTAATTCTGGATGATCCTGTTACCAGCAATAATATTTATGATACTTCACAAAACTCGGAGATATATGACCTTAAAATTTAGGTTTAAATACCATTATGGTTCTTATA

Coding sequence (CDS)

ATGGGTCCATTCCCATTGCTTGATACAGCAATTGAAATCTTTCCGCGTTGCCCTATTATCACTTCGGGTTCTTCATATGGCAGGCGCTCATCAAATTGCCATCACATTCTAAGAACAGTTTCAGCTACCAGGAACTGGAAAGTTTCATACATTGAAAACTTGCAGTCTAAACGAAACACTGTTGCCTTTTCTAGTCGGGACAATTCAGGTGATCATTTAACAGATTTGGTAGATGATGCTGATGGCTTTTCAATTGGTAGAAGTGAAGTATTGGAGACAGAAGAAGATGAAATTCTTGCAGTTAAAAAAGCGCTTTTGGAGTCTCAAACAAGACAAGAAGCTGTAGAGAAAGAGAGAGATCAACTGCTTGAGAGGTTAGCTCGCTATGAGGCTAAACAAAAAGAATATGTTGCTACTATATTGCATGATAAGGAATTGGCAGTTTCAGAACTCGAAGCTGCCAGATCATTATTTAATAAGAAACTTGAGGAATCAGTAGGTGAGAAGTTTGCTTTGGAGTCTAAGCTGGTCCTTGCAAAGCAGGATGCTATTGATCTTGCAGTGCAGGTTGAAAAGTTAGCCGCAATTGCCTTCCAGCAGGCCACTTCACACATATTAGAAGATGCTCAATACAGAGTATCAGTTGCAGAAACTTCTGCTGTTGAGACGTCTTATGATATTGAAAAACAGATCAGGGATGCTACTGAAGGTTCAATGCTGTCATTTGTAGAACAATCAAAAATAGCTATTGAGAAGGCACTGGATGTGGCTGAGAAAGCTAGTGCTCATGCAAAGAAGGCTGTGGCTACATTTACTGATGAGGTATATCCTTTAGATGAGATTGCCTCTATCCAATCAGAAAATATTAAGTTGAAAGGTGTCGTCAATGAAATGGAATCTCACTTATCACTTGCAAGAACTGATATTGATAACCTCAAGTTGGAACTGGAGCAAGCCCGAGCACAAGCAACTGCATCAGAAATTCGAGCCAAAAATGCTGAGAAAGCATTGGTCGAATTCCAGGAGTTGAGCAGGGAAAAGATCATTCAACAAGAGGGAGAAATTAAATTGATGATGGAGAAAATCAAGAAAGATGTAGCAGACAAGAAGAAAGCTGCTTCCAAAGCTTTCAAGGCTGAGCTTGAAGGTATTAAGTCTGCCATTCATGCTGCCAAAGAAACTGCACATTCCAAAGATAACGCCTATATGAGAAGATGTGAAGCACTGCAAAGATTATTGAGGGCGTCGGAAGCTGCAACAAAGATGTGGCAACAACGAGCGGATATGGCGGAGTCATTTTTATTGAAGGAAAGAACCATGGGTAAAGATAATGAAGTTGCAGCTTATATTGTCAATGGTGGGCGGATAGACCTCTTGACAGATGATGAGTCACAAAAGTGGAAACTCTTGAGCGAAGGACCTCGAAGAGAGATACCTCAATGGATGGCTAGAAGAATTGGAACCATTCGTCCCAAGTTTCCTCCTAGAAAGATTGATTTAACTGAAATTTCAACATCAAAGTTTAGATCATTGGACTTGCCCAAACTTGAAGAAGTATGGTCAATTGCTCAAGAAAAGCCAAAAATAGGGGATACACTAATTGAGCATGTTATCGAGAAAGAAACAATAGAAAAGAAAAGAAAGGCTCTTGAGCGTGCACTGCAACGAAAGACCATACAATGGCAGAGGACCCCTGATCAAACAAAATTGGAACCAGGAACTGGGACTGGACACGAAATTGTGTTTCAAGGTTTCAACTGGGAAAGCTGGAGAAGACGCTGGTACCTGGAGTTAGCTGCCAAAGCATCTGATTTATCTCAGTCTGGGATAACAGCAGTGTGGCTCCCTCCACCAACAGAATCTGTTGCTCCACAAGGTTATATGCCATCTGACCTATATAATTTGAACTCATCTTATGGATCTGAGGAAGAACTCAAGTTCTGTATAGAAGAATTTCATTCTCAAGATCTTCTGGCCCTCGGTGATGTTGTACTCAATCATAGATGTGCACACAAGCAGAGTCCCAATGGGGTTTGGAACATTTTCGGTGGTAAGCTTACTTGGGGACCTGAAGCAATTGTTTGTGATGATCCGAATTTTCAGGGTCGAGGAAATCCTTCAAGTGGCGACATCTTCCATGCGGCACCAAATATTGATCATTCGCAGGACTTTGTGAGGAGAGACATAAAAGAATGGCTAAATTGGCTTCGCAATGATATTGGTTTTGATGGGTGGCGCCTTGATTTTGTGAGGGGTTTCTCGGGTACATATGTTAAAGAATATATTGAGACTTCAAATCCTGCTTTTGCCATCGGAGAATACTGGGACAGTTTGGCTTATGAACATGGGAATTTATGTTATAACCAAGATGCTCATCGGCAACGAATAGTTGATTGGATCAATGCCACAGGTGGCACTTCCTCTGCATTCGATGTTACCACCAAGGGGATATTGCATTCTGCACTTCATAACCAATATTGGAGGTTGATAGACCCACAAGGAAAACCAACAGGAGTAGTGGGATGGTGGCCTTCCCGTGCTGTCACTTTCTTGGAAAACCATGACACTGGTTCCACACAGGGACACTGGCCATTTCCACGTGATAAACTTGCTCAAGGATATGCATATATCTTGACTCATCCTGGAACGCCTACAATTTTCTACGACCACTTTTATGATTTTGGGATTCGTGAAATAATCAATGAGTTAATCGAGGCTCGACAACGTGCTGAGATCCATTGTCGGAGCTCTGTTAAGATCTACCATGCAAATAATGAAGGATATGTTGCACAGGTTGGAGATACACTGGTCATGAAGCTTGGACATTTTGATTGGAATCCCTCGAAGGAAAATCATTTGGATGGGAATTGGCAGAAGTTTGTTGACAAAGGATCAGATTACCAATTGTGGCTGAGACAATAA

Protein sequence

MGPFPLLDTAIEIFPRCPIITSGSSYGRRSSNCHHILRTVSATRNWKVSYIENLQSKRNTVAFSSRDNSGDHLTDLVDDADGFSIGRSEVLETEEDEILAVKKALLESQTRQEAVEKERDQLLERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAKQDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVETSYDIEKQIRDATEGSMLSFVEQSKIAIEKALDVAEKASAHAKKAVATFTDEVYPLDEIASIQSENIKLKGVVNEMESHLSLARTDIDNLKLELEQARAQATASEIRAKNAEKALVEFQELSREKIIQQEGEIKLMMEKIKKDVADKKKAASKAFKAELEGIKSAIHAAKETAHSKDNAYMRRCEALQRLLRASEAATKMWQQRADMAESFLLKERTMGKDNEVAAYIVNGGRIDLLTDDESQKWKLLSEGPRREIPQWMARRIGTIRPKFPPRKIDLTEISTSKFRSLDLPKLEEVWSIAQEKPKIGDTLIEHVIEKETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLALGDVVLNHRCAHKQSPNGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDHSQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAYEHGNLCYNQDAHRQRIVDWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREIINELIEARQRAEIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKFVDKGSDYQLWLRQ
Homology
BLAST of Lsi07G010040 vs. ExPASy Swiss-Prot
Match: Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 2.0e-154
Identity = 262/488 (53.69%), Postives = 336/488 (68.85%), Query Frame = 0

Query: 494 PPRKIDLTEISTSKFRSLDLPKLE----EVWSIAQEKPKIGDTLIEHVIEKETIEKKRKA 553
           P RK D  E+S S F    + ++     ++ S   +K  + +     + E E +  +  +
Sbjct: 403 PKRKTD-KEVSASGFTKEIITEIRNLAIDISSHKNQKTNVKEVQENILQEIEKLAAEAYS 462

Query: 554 LERAL-----QRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRR-RWYLELAAKAS 613
           + R+      +   ++ +      K+  GTG+G EI+ QGFNWES +  RWYLEL  KA 
Sbjct: 463 IFRSTTPAFSEEGVLEAEADKPDIKISSGTGSGFEILCQGFNWESNKSGRWYLELQEKAD 522

Query: 614 DLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLALGDV 673
           +L+  G T +WLPPPTESV+P+GYMP DLYNLNS YG+ +ELK  +++FH   +  LGD 
Sbjct: 523 ELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDA 582

Query: 674 VLNHRCAHKQSPNGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDHSQD 733
           VLNHRCAH ++ NGVWN+FGG+L W   A+V DDP+FQGRGN SSGD FHAAPNIDHSQD
Sbjct: 583 VLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDPHFQGRGNKSSGDNFHAAPNIDHSQD 642

Query: 734 FVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAYEHG 793
           FVR+DIKEWL W+  ++G+DGWRLDFVRGF G YVK+Y++ S P FA+GEYWDSL+Y +G
Sbjct: 643 FVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYG 702

Query: 794 NLCYNQDAHRQRIVDWINATGGTSSAFDVTTKGILHSALHN-QYWRLIDPQGKPTGVVGW 853
            + YNQDAHRQRIVDWINAT G + AFDVTTKGILH+AL   +YWRL DP+GKP GVVGW
Sbjct: 703 EMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGW 762

Query: 854 WPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREIINE 913
           WPSRAVTF+ENHDTGSTQGHW FP  K  QGYAYILTHPGTP +F+DH +     EI   
Sbjct: 763 WPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAVFFDHIFSDYHSEIA-A 822

Query: 914 LIEARQRAEIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKFVD 971
           L+  R R ++HCRS V I  +  + Y A + + + MK+G   + P   +    NW   V+
Sbjct: 823 LLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQ---NWSVAVE 882

BLAST of Lsi07G010040 vs. ExPASy Swiss-Prot
Match: Q8LFG1 (Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 3.1e-123
Identity = 206/409 (50.37%), Postives = 268/409 (65.53%), Query Frame = 0

Query: 566 DQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAAKASDLSQSGITAVWLPPPTESVAPQ 625
           DQT +      G E++ Q +NWES +  W+  L  K  D+++SG T+ WLPPP++S+AP+
Sbjct: 13  DQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPE 72

Query: 626 GYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLALGDVVLNHRCAHKQSPNGVWNIFGG- 685
           GY+P DLY+LNS+YGSE  LK  + +     + A+ D+V+NHR    +   G++N + G 
Sbjct: 73  GYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGI 132

Query: 686 KLTWGPEAIV-CDDPNFQGRGNPSSGDIFHAAPNIDHSQDFVRRDIKEWLNWLRNDIGFD 745
            L W   A+  C      G GN S+GD F+  PN+DH+Q FVR+DI  WL WLRN +GF 
Sbjct: 133 SLPWDEHAVTSCTG----GLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQ 192

Query: 746 GWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAYEHGNLCYNQDAHRQRIVDWINAT 805
            +R DF RG+S  YVKEYI  + P F++GE WDS  Y    L YNQD+HRQRI+ WI+AT
Sbjct: 193 DFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDAT 252

Query: 806 GGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTGSTQGHW 865
           G  S+AFD TTKGIL  A+  QYWRL D QGKP GV+GWWPSRAVTFL+NHDTGSTQ HW
Sbjct: 253 GQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHW 312

Query: 866 PFPRDKLAQGYAYILTHPGTPTIFYDHFYDFG--IREIINELIEARQRAEIHCRSSVKIY 925
           PFP   + +GYAYILTHPG P +FYDHFYD+G  I + I +LI+ R+R +IH RS+V++ 
Sbjct: 313 PFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVL 372

Query: 926 HANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKFVDKGSDYQLW 971
            A +  Y A VG+ + MKLG   W PS      G        G  Y +W
Sbjct: 373 KAESNLYAAIVGEKICMKLGDGSWCPS------GRDWTLATSGHRYAVW 411

BLAST of Lsi07G010040 vs. ExPASy Swiss-Prot
Match: A2YGY2 (Alpha-amylase isozyme 2A OS=Oryza sativa subsp. indica OX=39946 GN=AMYC2 PE=2 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 1.1e-115
Identity = 200/384 (52.08%), Postives = 262/384 (68.23%), Query Frame = 0

Query: 573 GTGTGHEIVFQGFNWESWRRR--WYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPS 632
           G  +G +I+FQGFNWESWR+   WY  L  K  D+  +G+T VWLPPP+ SV+ QGYMP 
Sbjct: 17  GLASGDKILFQGFNWESWRQSGGWYNLLMGKVDDIVAAGVTHVWLPPPSHSVSTQGYMPG 76

Query: 633 DLYNLNSS-YGSEEELKFCIEEFHSQDLLALGDVVLNHRCAHKQSPNGVWNIF-----GG 692
            LY+L++S YG+  ELK  I   H + + A+ DVV+NHRCA  +   G++ IF      G
Sbjct: 77  RLYDLDASRYGTSMELKSLISALHGKGIQAIADVVINHRCADYKDSRGIYCIFEGGTPDG 136

Query: 693 KLTWGPEAIVCDDPNF-QGRGNPSSGDIFHAAPNIDHSQDFVRRDIKEWLNWLRND-IGF 752
           +L WGP  I  DD  F  G GN  +G  F AAP+IDH    V+R++ +WL WL++D +GF
Sbjct: 137 RLDWGPHMICRDDTQFSDGTGNLDTGADFAAAPDIDHLNGVVQRELTDWLLWLKSDEVGF 196

Query: 753 DGWRLDFVRGFSGTYVKEYIETSNP-AFAIGEYWDSLAY-EHGNLCYNQDAHRQRIVDWI 812
           D WRLDF RG+S    K YIE + P   A+ E WDS+AY   G   YNQDAHRQ +VDW+
Sbjct: 197 DAWRLDFARGYSPEVAKVYIEGTTPVGLAVAELWDSMAYGGDGKPEYNQDAHRQALVDWV 256

Query: 813 NATGGTSSA---FDVTTKGILHSALHNQYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTG 872
           +  GGT+SA   FD TTKGI+++A+  + WRLID QGK  GV+GWWP++AVTF++NHDTG
Sbjct: 257 DRVGGTASAGMVFDFTTKGIMNTAVEGELWRLIDQQGKAPGVIGWWPAKAVTFVDNHDTG 316

Query: 873 STQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREIINELIEARQRAEIHCRSS 932
           STQ  WPFP DK+ QGYAYILTHPG P IFYDHF+D+G++E I  L+  RQR  +   SS
Sbjct: 317 STQQMWPFPSDKVMQGYAYILTHPGNPCIFYDHFFDWGLKEQIAALVAVRQRNGVTATSS 376

Query: 933 VKIYHANNEGYVAQVGDTLVMKLG 942
           +KI   + + YVA++   +VMK+G
Sbjct: 377 LKIMLHDADAYVAEIDGKVVMKIG 400

BLAST of Lsi07G010040 vs. ExPASy Swiss-Prot
Match: Q0D9J1 (Alpha-amylase isozyme 2A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY2A PE=2 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 1.1e-115
Identity = 200/384 (52.08%), Postives = 262/384 (68.23%), Query Frame = 0

Query: 573 GTGTGHEIVFQGFNWESWRRR--WYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPS 632
           G  +G +I+FQGFNWESWR+   WY  L  K  D+  +G+T VWLPPP+ SV+ QGYMP 
Sbjct: 17  GLASGDKILFQGFNWESWRQSGGWYNLLMGKVDDIVAAGVTHVWLPPPSHSVSTQGYMPG 76

Query: 633 DLYNLNSS-YGSEEELKFCIEEFHSQDLLALGDVVLNHRCAHKQSPNGVWNIF-----GG 692
            LY+L++S YG+  ELK  I   H + + A+ DVV+NHRCA  +   G++ IF      G
Sbjct: 77  RLYDLDASRYGTSMELKSLISALHGKGIQAIADVVINHRCADYKDSRGIYCIFEGGTPDG 136

Query: 693 KLTWGPEAIVCDDPNF-QGRGNPSSGDIFHAAPNIDHSQDFVRRDIKEWLNWLRND-IGF 752
           +L WGP  I  DD  F  G GN  +G  F AAP+IDH    V+R++ +WL WL++D +GF
Sbjct: 137 RLDWGPHMICRDDTQFSDGTGNLDTGADFAAAPDIDHLNGVVQRELTDWLLWLKSDEVGF 196

Query: 753 DGWRLDFVRGFSGTYVKEYIETSNP-AFAIGEYWDSLAY-EHGNLCYNQDAHRQRIVDWI 812
           D WRLDF RG+S    K YIE + P   A+ E WDS+AY   G   YNQDAHRQ +VDW+
Sbjct: 197 DAWRLDFARGYSPEVAKVYIEGTTPVGLAVAELWDSMAYGGDGKPEYNQDAHRQALVDWV 256

Query: 813 NATGGTSSA---FDVTTKGILHSALHNQYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTG 872
           +  GGT+SA   FD TTKGI+++A+  + WRLID QGK  GV+GWWP++AVTF++NHDTG
Sbjct: 257 DRVGGTASAGMVFDFTTKGIMNTAVEGELWRLIDQQGKAPGVIGWWPAKAVTFVDNHDTG 316

Query: 873 STQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREIINELIEARQRAEIHCRSS 932
           STQ  WPFP DK+ QGYAYILTHPG P IFYDHF+D+G++E I  L+  RQR  +   SS
Sbjct: 317 STQQMWPFPSDKVMQGYAYILTHPGNPCIFYDHFFDWGLKEQIAALVAVRQRNGVTATSS 376

Query: 933 VKIYHANNEGYVAQVGDTLVMKLG 942
           +KI   + + YVA++   +VMK+G
Sbjct: 377 LKIMLHDADAYVAEIDGKVVMKIG 400

BLAST of Lsi07G010040 vs. ExPASy Swiss-Prot
Match: P00693 (Alpha-amylase type A isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.1 PE=1 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 4.4e-114
Identity = 187/382 (48.95%), Postives = 263/382 (68.85%), Query Frame = 0

Query: 573 GTGTGHEIVFQGFNWESWRRR--WYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPS 632
           G  +GH+++FQGFNWESW++   WY  +  K  D++ +G+T VWLPPP+ SV+ +GYMP 
Sbjct: 20  GLASGHQVLFQGFNWESWKQSGGWYNMMMGKVDDIAAAGVTHVWLPPPSHSVSNEGYMPG 79

Query: 633 DLYNLNSS-YGSEEELKFCIEEFHSQDLLALGDVVLNHRCAHKQSPNGVWNIF-----GG 692
            LY++++S YG+  ELK  I   H + + A+ D+V+NHRCA  +   G++ IF      G
Sbjct: 80  RLYDIDASKYGNAAELKSLIGALHGKGVQAIADIVINHRCADYKDSRGIYCIFEGGTSDG 139

Query: 693 KLTWGPEAIVCDDPNF-QGRGNPSSGDIFHAAPNIDHSQDFVRRDIKEWLNWLRNDIGFD 752
           +L WGP  I  DD  +  G  N  +G  F AAP+IDH  D V+R++KEWL WL++D+GFD
Sbjct: 140 RLDWGPHMICRDDTKYSDGTANLDTGADFAAAPDIDHLNDRVQRELKEWLLWLKSDLGFD 199

Query: 753 GWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY-EHGNLCYNQDAHRQRIVDWINA 812
            WRLDF RG+S    K YI+ ++P+ A+ E WD++A    G   Y+QDAHRQ +V+W++ 
Sbjct: 200 AWRLDFARGYSPEMAKVYIDGTSPSLAVAEVWDNMATGGDGKPNYDQDAHRQNLVNWVDK 259

Query: 813 TGGTSSA---FDVTTKGILHSALHNQYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTGST 872
            GG +SA   FD TTKGIL++A+  + WRLIDPQGK  GV+GWWP++A TF++NHDTGST
Sbjct: 260 VGGAASAGMVFDFTTKGILNAAVEGELWRLIDPQGKAPGVMGWWPAKAATFVDNHDTGST 319

Query: 873 QGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREIINELIEARQRAEIHCRSSVK 932
           Q  WPFP DK+ QGYAYILTHPG P IFYDHF+++G ++ I  L+  R+R  I   S++K
Sbjct: 320 QAMWPFPSDKVMQGYAYILTHPGIPCIFYDHFFNWGFKDQIAALVAIRKRNGITATSALK 379

Query: 933 IYHANNEGYVAQVGDTLVMKLG 942
           I     + YVA++   +V+K+G
Sbjct: 380 ILMHEGDAYVAEIDGKVVVKIG 401

BLAST of Lsi07G010040 vs. ExPASy TrEMBL
Match: A0A1S3C1E0 (1,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo OX=3656 GN=LOC103495777 PE=4 SV=1)

HSP 1 Score: 1853.6 bits (4800), Expect = 0.0e+00
Identity = 927/973 (95.27%), Postives = 950/973 (97.64%), Query Frame = 0

Query: 1   MGPFPLLDTAIEIFPRCPIITSGSSYGRRSSNCHHILRTVSATRNWKVSYIENLQSKRNT 60
           MGPFPLLDTAIEIFPRCPIITS SSYGRRSS+CH ++ TVSATRNWKVSYIENLQSK  T
Sbjct: 1   MGPFPLLDTAIEIFPRCPIITSRSSYGRRSSHCHLLVTTVSATRNWKVSYIENLQSKPKT 60

Query: 61  VAFSSRDNSGDHLTDLVDDADGFSIGRSEVLETEEDEILAVKKALLESQTRQEAVEKERD 120
           V FSSRDNS DHLTDLV+DADGF+ GRSEVLET EDEILAVKKALLESQTRQ+AVEKERD
Sbjct: 61  VVFSSRDNSNDHLTDLVNDADGFTTGRSEVLETGEDEILAVKKALLESQTRQKAVEKERD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180
           QLLERLARYEAKQKEYVATILHDKELA+SELEAARSLFNKKLEESVGEKFALESKLVLAK
Sbjct: 121 QLLERLARYEAKQKEYVATILHDKELAISELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVETSYDIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSA+ETSY+IEKQIRDATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML 240

Query: 241 SFVEQSKIAIEKALDVAEKASAHAKKAVATFTDEVYPLDEIASIQSENIKLKGVVNEMES 300
           SF+EQSKIAIEKALDVAEKAS HAKKA+ATFTDEVYPLD I SIQSENIKLKGVVNE+ES
Sbjct: 241 SFLEQSKIAIEKALDVAEKASVHAKKAMATFTDEVYPLDGITSIQSENIKLKGVVNELES 300

Query: 301 HLSLARTDIDNLKLELEQARAQATASEIRAKNAEKALVEFQELSREKIIQQEGEIKLMME 360
           HLSLARTD+DNLKLELE ARAQATASEIRAKNAEK LVEFQELSREKI QQEGEIKLMME
Sbjct: 301 HLSLARTDVDNLKLELENARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIHAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKDVADKKKAASKAFK ELEGIKSAI AAKETAHSKD+AYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDVADKKKAASKAFKVELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESFLLKERTMGKDNEVAAYIVNGGRIDLLTDDESQKWKLLSEGPRREIPQ 480
           KMWQQRADMAESFLLKERTMGKDNE AAYIVNGGRIDLLTDDESQKWKLL++GPRREIPQ
Sbjct: 421 KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLTDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDLTEISTSKFRSLDLPKLEEVWSIAQEKPKIGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKID+TEIS SKFRSLDLPKLEEVWSIAQEKPK+GD LIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDVTEISASKFRSLDLPKLEEVWSIAQEKPKVGDALIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           ETIEKKRKALERALQRKT QWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ETIEKKRKALERALQRKTKQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYG+EEELK+CIEEFHSQDLLAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKL WGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720

Query: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780
           SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY
Sbjct: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780

Query: 781 EHGNLCYNQDAHRQRIVDWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EHGNLCYNQDAHRQRIV+WINATGGTSSAFDVTTKGILHSALHNQYWR+IDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRMIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIRE+I
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREMI 900

Query: 901 NELIEARQRAEIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
           NELIEARQRA IHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF
Sbjct: 901 NELIEARQRAGIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLWLRQ
Sbjct: 961 VDKGSDYQLWLRQ 973

BLAST of Lsi07G010040 vs. ExPASy TrEMBL
Match: A0A6J1G955 (1,4-alpha-D-glucan glucanohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111452072 PE=4 SV=1)

HSP 1 Score: 1771.1 bits (4586), Expect = 0.0e+00
Identity = 888/973 (91.26%), Postives = 921/973 (94.66%), Query Frame = 0

Query: 1   MGPFPLLDTAIEIFPRCPIITSGSSYGRRSSNCHHILRTVSATRNWKVSYIENLQSKRNT 60
           MGPFPLLD AIEI PRCPI+T GSSYGRRSSNCHH LRTV+ T   KVSY +N   K  T
Sbjct: 1   MGPFPLLDAAIEILPRCPIVTPGSSYGRRSSNCHHNLRTVTVTWKRKVSYTDNFLHKPIT 60

Query: 61  VAFSSRDNSGDHLTDLVDDADGFSIGRSEVLETEEDEILAVKKALLESQTRQEAVEKERD 120
           VAFSSRD+S D            S GRSE+L +EEDEILAVKKALLESQTRQEAVEKE D
Sbjct: 61  VAFSSRDHSNDP-----------STGRSEILGSEEDEILAVKKALLESQTRQEAVEKETD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180
           QLLE+L RYEAKQKEY+ATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK
Sbjct: 121 QLLEKLTRYEAKQKEYLATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVETSYDIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAF+QATSHILEDAQ RVS AETSAVE SY+IEKQI DATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFEQATSHILEDAQLRVSAAETSAVEASYEIEKQISDATEGSML 240

Query: 241 SFVEQSKIAIEKALDVAEKASAHAKKAVATFTDEVYPLDEIASIQSENIKLKGVVNEMES 300
           SFVEQSKIAIEKALDVAEKASAHA KAVATFTDEVYPLDEIASIQSE++KLKGVV+E+ES
Sbjct: 241 SFVEQSKIAIEKALDVAEKASAHANKAVATFTDEVYPLDEIASIQSESVKLKGVVDELES 300

Query: 301 HLSLARTDIDNLKLELEQARAQATASEIRAKNAEKALVEFQELSREKIIQQEGEIKLMME 360
           HLSLAR D+DNLKLELEQARAQATASEIRAKNAEKAL+EFQ  S EKIIQQEGEIKLMME
Sbjct: 301 HLSLARADVDNLKLELEQARAQATASEIRAKNAEKALLEFQNSSMEKIIQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIHAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKD  DKKKAASKAFKAELEGIKSAI AAKETAHSKDNAYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDFTDKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESFLLKERTMGKDNEVAAYIVNGGRIDLLTDDESQKWKLLSEGPRREIPQ 480
           KMWQQRA+MAESFL KERT+GKDNE AAY+VNGGRIDLLT+DESQKWKLLS+GPRREIPQ
Sbjct: 421 KMWQQRAEMAESFLSKERTLGKDNEEAAYVVNGGRIDLLTNDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDLTEISTSKFRSLDLPKLEEVWSIAQEKPKIGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKID++E+STSKFRSLDLPKLEEVWSIAQEKPK+GDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDISEVSTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           E+IEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ESIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELK+CIEEFHSQDLLAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKL WGPEAIVCDDPNFQGRGNP SGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLAWGPEAIVCDDPNFQGRGNPKSGDIFHAAPNIDH 720

Query: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780
           SQDFVR+DIKEWLNWLR+DIGFDGWRLDFVRGFSGTYVKEYIETSNP FAIGEYWDSLAY
Sbjct: 721 SQDFVRKDIKEWLNWLRSDIGFDGWRLDFVRGFSGTYVKEYIETSNPTFAIGEYWDSLAY 780

Query: 781 EHGNLCYNQDAHRQRIVDWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EHGNLCYNQDAHRQRIV+WINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKL QGYAYILTHPGTPT+FYDHFYDFGIREII
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLVQGYAYILTHPGTPTVFYDHFYDFGIREII 900

Query: 901 NELIEARQRAEIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
           NELIEARQRA IHCRSSVKI+HANNEGYVAQVGDTLVMKLGHFDWNPSKEN LDG WQKF
Sbjct: 901 NELIEARQRAGIHCRSSVKIFHANNEGYVAQVGDTLVMKLGHFDWNPSKENQLDGKWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLW+RQ
Sbjct: 961 VDKGSDYQLWMRQ 962

BLAST of Lsi07G010040 vs. ExPASy TrEMBL
Match: A0A6J1KAV6 (1,4-alpha-D-glucan glucanohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111492635 PE=4 SV=1)

HSP 1 Score: 1758.8 bits (4554), Expect = 0.0e+00
Identity = 884/973 (90.85%), Postives = 915/973 (94.04%), Query Frame = 0

Query: 1   MGPFPLLDTAIEIFPRCPIITSGSSYGRRSSNCHHILRTVSATRNWKVSYIENLQSKRNT 60
           MGPFPLLD AIEI PRCPI+T GSSYGRRSSNCHH LRTVS T   KVSY +N   K  T
Sbjct: 1   MGPFPLLDVAIEILPRCPIVTPGSSYGRRSSNCHHNLRTVSVTWKRKVSYTDNFLHKPIT 60

Query: 61  VAFSSRDNSGDHLTDLVDDADGFSIGRSEVLETEEDEILAVKKALLESQTRQEAVEKERD 120
           VAFSSRDNS D            S G SE+L +EEDEILAVKKALLESQTRQEAVEKE D
Sbjct: 61  VAFSSRDNSNDP-----------STGGSEILGSEEDEILAVKKALLESQTRQEAVEKETD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180
           QLLE+L RYEAKQKEY+ATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK
Sbjct: 121 QLLEKLTRYEAKQKEYLATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVETSYDIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAF+QATSHILEDAQ RVS AETSAVE SY+IEKQI DATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFEQATSHILEDAQLRVSAAETSAVEASYEIEKQISDATEGSML 240

Query: 241 SFVEQSKIAIEKALDVAEKASAHAKKAVATFTDEVYPLDEIASIQSENIKLKGVVNEMES 300
           SFVEQSKIAIEKALDVAEKASAHA KAVATFTDEVY LDEIASIQSE++KLKGVV+E+ES
Sbjct: 241 SFVEQSKIAIEKALDVAEKASAHANKAVATFTDEVYALDEIASIQSESVKLKGVVDELES 300

Query: 301 HLSLARTDIDNLKLELEQARAQATASEIRAKNAEKALVEFQELSREKIIQQEGEIKLMME 360
           HLSLAR D+DNLKLELEQARA+ATASEIRAKNAE  L+EFQ  S EKIIQQEGEIKLMME
Sbjct: 301 HLSLARADVDNLKLELEQARARATASEIRAKNAETVLLEFQNSSMEKIIQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIHAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKD  DKKKAASKAFKAELEGIKSAI AAKETAHSKDNAYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDFTDKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESFLLKERTMGKDNEVAAYIVNGGRIDLLTDDESQKWKLLSEGPRREIPQ 480
           KMWQQRA+MAESFL KERT+GKDNE AAYIVNGGRIDLLT+DESQKWKLLS+GPRR IPQ
Sbjct: 421 KMWQQRAEMAESFLSKERTLGKDNEEAAYIVNGGRIDLLTNDESQKWKLLSDGPRRAIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDLTEISTSKFRSLDLPKLEEVWSIAQEKPKIGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKID+TE+STSKFRSLDLPKLEEVWSIAQEKPK+GDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDITEVSTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           E+IEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ESIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELK+CIEEFHSQDLLAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKL WGPEAIVCDDPNFQGRGNP SGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLAWGPEAIVCDDPNFQGRGNPKSGDIFHAAPNIDH 720

Query: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780
           SQDFVR+DIKEWLNWLR+DIGFDGWRLDFVRGFSGTYVKEYIETS+P FAIGEYWDSLAY
Sbjct: 721 SQDFVRKDIKEWLNWLRSDIGFDGWRLDFVRGFSGTYVKEYIETSDPTFAIGEYWDSLAY 780

Query: 781 EHGNLCYNQDAHRQRIVDWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EHGNLCYNQDAHRQRIV+WINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKL QGYAYILTHPGTPT+FYDHFYDFGIREII
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLVQGYAYILTHPGTPTVFYDHFYDFGIREII 900

Query: 901 NELIEARQRAEIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
           NELIEARQRA IHCRSSVKI+HANNEGYVAQVGD LVMKLGHFDWNPSKEN LDG WQKF
Sbjct: 901 NELIEARQRAGIHCRSSVKIFHANNEGYVAQVGDNLVMKLGHFDWNPSKENQLDGKWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLW+RQ
Sbjct: 961 VDKGSDYQLWMRQ 962

BLAST of Lsi07G010040 vs. ExPASy TrEMBL
Match: A0A6J1DP26 (uncharacterized protein LOC111022188 OS=Momordica charantia OX=3673 GN=LOC111022188 PE=4 SV=1)

HSP 1 Score: 1604.3 bits (4153), Expect = 0.0e+00
Identity = 805/883 (91.17%), Postives = 838/883 (94.90%), Query Frame = 0

Query: 1   MGPFPLLDTAIEIFPRCPIITSGSSYGRRSSNCHHILRTVSATRNWKVSYIENLQSKRNT 60
           MGP PLLDTAIE  PRCPII++GSSY RRSSN H ILRTVSA R WK+SY++ L  K  T
Sbjct: 1   MGPLPLLDTAIEFLPRCPIISAGSSYCRRSSNYHQILRTVSAARKWKLSYVDKLLCKPKT 60

Query: 61  VAFSSRDNSGDHLTDLVDDADGFSIGRSEVLETEEDEILAVKKALLESQTRQEAVEKERD 120
           V FSS DNS D+LTD VDDADGFS GRSEVL+TEEDEILAVKK LLESQTRQEAVEKERD
Sbjct: 61  VVFSSPDNSSDNLTDWVDDADGFSTGRSEVLQTEEDEILAVKKVLLESQTRQEAVEKERD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180
           QL+ERLARYEAKQKEYVATILHDKELAVSELEAARSLFNK+L +SVGEKFALESKLVLAK
Sbjct: 121 QLIERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKRLHDSVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVETSYDIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVE SY IEKQIRDA EGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVEASYQIEKQIRDAIEGSML 240

Query: 241 SFVEQSKIAIEKALDVAEKASAHAKKAVATFTDEVYPLDEIASIQSENIKLKGVVNEMES 300
           SFVEQSK  IEKALDVAEKA AHAKK V TFTDEVYPLDEIAS+QSENIKLKGV+NE+ES
Sbjct: 241 SFVEQSKQTIEKALDVAEKAGAHAKKVVGTFTDEVYPLDEIASVQSENIKLKGVINELES 300

Query: 301 HLSLARTDIDNLKLELEQARAQATASEIRAKNAEKALVEFQELSREKIIQQEGEIKLMME 360
           HL L R D+DNLKLELEQARAQATASEIRAKN+EK L+EFQELSREKII+QEGEIK MME
Sbjct: 301 HLLLTRADVDNLKLELEQARAQATASEIRAKNSEKILLEFQELSREKIIKQEGEIKSMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIHAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           K KKD+ADKKKAA+KAFKAELEGIKSAI AAKETA+SKDNAY+RRCEALQRLLRASEAAT
Sbjct: 361 KFKKDLADKKKAATKAFKAELEGIKSAIEAAKETAYSKDNAYVRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESFLLKERTMGKDNEVAAYIVNGGRIDLLTDDESQKWKLLSEGPRREIPQ 480
           K WQQRADMAES LLKERT GK +E A Y+VNGGRIDLLTDDESQKWKLLS+GPRREIPQ
Sbjct: 421 KTWQQRADMAESLLLKERTPGKVDEDAVYVVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDLTEISTSKFRSLDLPKLEEVWSIAQEKPKIGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKID+TE+STSKFRSLDLPK+EEVWSIAQEKPK+GDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDVTEVSTSKFRSLDLPKIEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           ETIEKKRKALERAL+RKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ETIEKKRKALERALERKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELK+CIEEFHSQDLLAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKL WGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720

Query: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780
           SQDFVR+DIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIE SNP FAIGEYWDSL Y
Sbjct: 721 SQDFVRKDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLGY 780

Query: 781 EHGNLCYNQDAHRQRIVDWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EHGNLCYNQDAHRQRI++WINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIINWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGT 884
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGT
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGT 883

BLAST of Lsi07G010040 vs. ExPASy TrEMBL
Match: A0A6J1G9N4 (uncharacterized protein LOC111452072 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452072 PE=4 SV=1)

HSP 1 Score: 1589.3 bits (4114), Expect = 0.0e+00
Identity = 805/885 (90.96%), Postives = 835/885 (94.35%), Query Frame = 0

Query: 1   MGPFPLLDTAIEIFPRCPIITSGSSYGRRSSNCHHILRTVSATRNWKVSYIENLQSKRNT 60
           MGPFPLLD AIEI PRCPI+T GSSYGRRSSNCHH LRTV+ T   KVSY +N   K  T
Sbjct: 1   MGPFPLLDAAIEILPRCPIVTPGSSYGRRSSNCHHNLRTVTVTWKRKVSYTDNFLHKPIT 60

Query: 61  VAFSSRDNSGDHLTDLVDDADGFSIGRSEVLETEEDEILAVKKALLESQTRQEAVEKERD 120
           VAFSSRD+S D            S GRSE+L +EEDEILAVKKALLESQTRQEAVEKE D
Sbjct: 61  VAFSSRDHSNDP-----------STGRSEILGSEEDEILAVKKALLESQTRQEAVEKETD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180
           QLLE+L RYEAKQKEY+ATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK
Sbjct: 121 QLLEKLTRYEAKQKEYLATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVETSYDIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAF+QATSHILEDAQ RVS AETSAVE SY+IEKQI DATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFEQATSHILEDAQLRVSAAETSAVEASYEIEKQISDATEGSML 240

Query: 241 SFVEQSKIAIEKALDVAEKASAHAKKAVATFTDEVYPLDEIASIQSENIKLKGVVNEMES 300
           SFVEQSKIAIEKALDVAEKASAHA KAVATFTDEVYPLDEIASIQSE++KLKGVV+E+ES
Sbjct: 241 SFVEQSKIAIEKALDVAEKASAHANKAVATFTDEVYPLDEIASIQSESVKLKGVVDELES 300

Query: 301 HLSLARTDIDNLKLELEQARAQATASEIRAKNAEKALVEFQELSREKIIQQEGEIKLMME 360
           HLSLAR D+DNLKLELEQARAQATASEIRAKNAEKAL+EFQ  S EKIIQQEGEIKLMME
Sbjct: 301 HLSLARADVDNLKLELEQARAQATASEIRAKNAEKALLEFQNSSMEKIIQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIHAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKD  DKKKAASKAFKAELEGIKSAI AAKETAHSKDNAYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDFTDKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESFLLKERTMGKDNEVAAYIVNGGRIDLLTDDESQKWKLLSEGPRREIPQ 480
           KMWQQRA+MAESFL KERT+GKDNE AAY+VNGGRIDLLT+DESQKWKLLS+GPRREIPQ
Sbjct: 421 KMWQQRAEMAESFLSKERTLGKDNEEAAYVVNGGRIDLLTNDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDLTEISTSKFRSLDLPKLEEVWSIAQEKPKIGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKID++E+STSKFRSLDLPKLEEVWSIAQEKPK+GDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDISEVSTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           E+IEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ESIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELK+CIEEFHSQDLLAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKL WGPEAIVCDDPNFQGRGNP SGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLAWGPEAIVCDDPNFQGRGNPKSGDIFHAAPNIDH 720

Query: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780
           SQDFVR+DIKEWLNWLR+DIGFDGWRLDFVRGFSGTYVKEYIETSNP FAIGEYWDSLAY
Sbjct: 721 SQDFVRKDIKEWLNWLRSDIGFDGWRLDFVRGFSGTYVKEYIETSNPTFAIGEYWDSLAY 780

Query: 781 EHGNLCYNQDAHRQRIVDWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EHGNLCYNQDAHRQRIV+WINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPT 886
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKL QGYAYILTHPGT T
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLVQGYAYILTHPGTIT 874

BLAST of Lsi07G010040 vs. NCBI nr
Match: XP_038893540.1 (uncharacterized protein LOC120082441 [Benincasa hispida])

HSP 1 Score: 1858.6 bits (4813), Expect = 0.0e+00
Identity = 932/973 (95.79%), Postives = 949/973 (97.53%), Query Frame = 0

Query: 1   MGPFPLLDTAIEIFPRCPIITSGSSYGRRSSNCHHILRTVSATRNWKVSYIENLQSKRNT 60
           MGPFPLLDTA+EIFPRCPII   SSYGRRSSNC+HIL TVS TRNWKVSYI NL SK  T
Sbjct: 1   MGPFPLLDTALEIFPRCPIIIPASSYGRRSSNCYHILTTVSPTRNWKVSYIGNLHSKPKT 60

Query: 61  VAFSSRDNSGDHLTDLVDDADGFSIGRSEVLETEEDEILAVKKALLESQTRQEAVEKERD 120
           VAFSSRDNS D LTDLVDD DGFS GRSEVLETEEDEILAVK ALLESQTRQEAVEKERD
Sbjct: 61  VAFSSRDNSSDRLTDLVDDGDGFSTGRSEVLETEEDEILAVKMALLESQTRQEAVEKERD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180
           QLLERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKKLEESVGEKF+LESKLVLAK
Sbjct: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKKLEESVGEKFSLESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVETSYDIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVETSY+IEKQIRDATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVETSYEIEKQIRDATEGSML 240

Query: 241 SFVEQSKIAIEKALDVAEKASAHAKKAVATFTDEVYPLDEIASIQSENIKLKGVVNEMES 300
           SFVEQSKIAIEKALDVAEKASAHAKKAVATFTDEVYPLDEIASIQSENI+LKGVVNE+ES
Sbjct: 241 SFVEQSKIAIEKALDVAEKASAHAKKAVATFTDEVYPLDEIASIQSENIQLKGVVNELES 300

Query: 301 HLSLARTDIDNLKLELEQARAQATASEIRAKNAEKALVEFQELSREKIIQQEGEIKLMME 360
           HLSLARTD+D LKLELEQARAQATASEIRAKNAEKA VEFQELSREK IQQEGEIKLMME
Sbjct: 301 HLSLARTDVDTLKLELEQARAQATASEIRAKNAEKAWVEFQELSREKTIQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIHAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKDVADKKKAASKAFKAELEGIKSAIHAAKETAHSKDNAYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDVADKKKAASKAFKAELEGIKSAIHAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESFLLKERTMGKDNEVAAYIVNGGRIDLLTDDESQKWKLLSEGPRREIPQ 480
           KMWQQRADMAES+LLKERT+G DNE AAYIVNGGRIDLLTDDESQKWKLLS+GPRREIPQ
Sbjct: 421 KMWQQRADMAESYLLKERTLGIDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDLTEISTSKFRSLDLPKLEEVWSIAQEKPKIGDTLIEHVIEK 540
           WMARRIGTIRP FPPRKID+TEISTSKFRSLDLPKLEEVWSIAQEKPK+GDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPMFPPRKIDITEISTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           ETIEKKRKALERALQRKTIQWQRTPD+TKLEPGTGTGHEIVFQGFNWESWRRRWYLELA 
Sbjct: 541 ETIEKKRKALERALQRKTIQWQRTPDKTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAT 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELK+CIEEFHSQDLLAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKL WGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720

Query: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780
           SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY
Sbjct: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780

Query: 781 EHGNLCYNQDAHRQRIVDWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EHG+LCYNQDAHRQRIV+WINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV
Sbjct: 781 EHGDLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIRE+I
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREMI 900

Query: 901 NELIEARQRAEIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
           NELIE RQRA IHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF
Sbjct: 901 NELIEVRQRAGIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLWLRQ
Sbjct: 961 VDKGSDYQLWLRQ 973

BLAST of Lsi07G010040 vs. NCBI nr
Match: XP_008455663.1 (PREDICTED: uncharacterized protein LOC103495777 [Cucumis melo] >XP_008455664.1 PREDICTED: uncharacterized protein LOC103495777 [Cucumis melo] >XP_008455665.1 PREDICTED: uncharacterized protein LOC103495777 [Cucumis melo] >XP_016901783.1 PREDICTED: uncharacterized protein LOC103495777 [Cucumis melo])

HSP 1 Score: 1853.6 bits (4800), Expect = 0.0e+00
Identity = 927/973 (95.27%), Postives = 950/973 (97.64%), Query Frame = 0

Query: 1   MGPFPLLDTAIEIFPRCPIITSGSSYGRRSSNCHHILRTVSATRNWKVSYIENLQSKRNT 60
           MGPFPLLDTAIEIFPRCPIITS SSYGRRSS+CH ++ TVSATRNWKVSYIENLQSK  T
Sbjct: 1   MGPFPLLDTAIEIFPRCPIITSRSSYGRRSSHCHLLVTTVSATRNWKVSYIENLQSKPKT 60

Query: 61  VAFSSRDNSGDHLTDLVDDADGFSIGRSEVLETEEDEILAVKKALLESQTRQEAVEKERD 120
           V FSSRDNS DHLTDLV+DADGF+ GRSEVLET EDEILAVKKALLESQTRQ+AVEKERD
Sbjct: 61  VVFSSRDNSNDHLTDLVNDADGFTTGRSEVLETGEDEILAVKKALLESQTRQKAVEKERD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180
           QLLERLARYEAKQKEYVATILHDKELA+SELEAARSLFNKKLEESVGEKFALESKLVLAK
Sbjct: 121 QLLERLARYEAKQKEYVATILHDKELAISELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVETSYDIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSA+ETSY+IEKQIRDATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML 240

Query: 241 SFVEQSKIAIEKALDVAEKASAHAKKAVATFTDEVYPLDEIASIQSENIKLKGVVNEMES 300
           SF+EQSKIAIEKALDVAEKAS HAKKA+ATFTDEVYPLD I SIQSENIKLKGVVNE+ES
Sbjct: 241 SFLEQSKIAIEKALDVAEKASVHAKKAMATFTDEVYPLDGITSIQSENIKLKGVVNELES 300

Query: 301 HLSLARTDIDNLKLELEQARAQATASEIRAKNAEKALVEFQELSREKIIQQEGEIKLMME 360
           HLSLARTD+DNLKLELE ARAQATASEIRAKNAEK LVEFQELSREKI QQEGEIKLMME
Sbjct: 301 HLSLARTDVDNLKLELENARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIHAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKDVADKKKAASKAFK ELEGIKSAI AAKETAHSKD+AYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDVADKKKAASKAFKVELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESFLLKERTMGKDNEVAAYIVNGGRIDLLTDDESQKWKLLSEGPRREIPQ 480
           KMWQQRADMAESFLLKERTMGKDNE AAYIVNGGRIDLLTDDESQKWKLL++GPRREIPQ
Sbjct: 421 KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLTDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDLTEISTSKFRSLDLPKLEEVWSIAQEKPKIGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKID+TEIS SKFRSLDLPKLEEVWSIAQEKPK+GD LIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDVTEISASKFRSLDLPKLEEVWSIAQEKPKVGDALIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           ETIEKKRKALERALQRKT QWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ETIEKKRKALERALQRKTKQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYG+EEELK+CIEEFHSQDLLAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKL WGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720

Query: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780
           SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY
Sbjct: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780

Query: 781 EHGNLCYNQDAHRQRIVDWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EHGNLCYNQDAHRQRIV+WINATGGTSSAFDVTTKGILHSALHNQYWR+IDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRMIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIRE+I
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREMI 900

Query: 901 NELIEARQRAEIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
           NELIEARQRA IHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF
Sbjct: 901 NELIEARQRAGIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLWLRQ
Sbjct: 961 VDKGSDYQLWLRQ 973

BLAST of Lsi07G010040 vs. NCBI nr
Match: XP_004137176.1 (uncharacterized protein LOC101217339 [Cucumis sativus] >KAE8649296.1 hypothetical protein Csa_014829 [Cucumis sativus])

HSP 1 Score: 1847.4 bits (4784), Expect = 0.0e+00
Identity = 926/973 (95.17%), Postives = 950/973 (97.64%), Query Frame = 0

Query: 1   MGPFPLLDTAIEIFPRCPIITSGSSYGRRSSNCHHILRTVSATRNWKVSYIENLQSKRNT 60
           MGPFPLLD AIEI PRCPIITS SSYGRRSS+CH  L  VS+TR WKVSYIENLQSK  T
Sbjct: 1   MGPFPLLDAAIEISPRCPIITSRSSYGRRSSHCHLRLTAVSSTRTWKVSYIENLQSKPKT 60

Query: 61  VAFSSRDNSGDHLTDLVDDADGFSIGRSEVLETEEDEILAVKKALLESQTRQEAVEKERD 120
           VAFSSRDNS DHLTDLV+DADGFS GRSEVLET EDEILAVKKALLESQTRQEAVEKERD
Sbjct: 61  VAFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGEDEILAVKKALLESQTRQEAVEKERD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180
           QLLERLARYEAKQKEYVATILHDKELAVSELE ARSLFNKKLEESVGEKFALESKLVLAK
Sbjct: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVETSYDIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSA+ETSY+IEKQIRDATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML 240

Query: 241 SFVEQSKIAIEKALDVAEKASAHAKKAVATFTDEVYPLDEIASIQSENIKLKGVVNEMES 300
           SF+EQSKIAIEKALDVAEKASAHAKKA+ATFTDEVYPLDEIASIQSENIKLKGV+NE+ES
Sbjct: 241 SFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEIASIQSENIKLKGVINELES 300

Query: 301 HLSLARTDIDNLKLELEQARAQATASEIRAKNAEKALVEFQELSREKIIQQEGEIKLMME 360
           HLSLAR++++NLKLELEQARAQATASEIRAKNAEK LVEFQELSREKI QQEGEIKLMME
Sbjct: 301 HLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIHAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKDVADKKKAASK FKAELEGIKSAI AAKETAHSKD+AYMRRCEALQRLLRASEA T
Sbjct: 361 KIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAGT 420

Query: 421 KMWQQRADMAESFLLKERTMGKDNEVAAYIVNGGRIDLLTDDESQKWKLLSEGPRREIPQ 480
           KMWQQRADMAESFLLKERTMGKDNE AAYIVNGGRIDLLTDDESQKWKLLS+GPRREIPQ
Sbjct: 421 KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDLTEISTSKFRSLDLPKLEEVWSIAQEKPKIGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKID+TEIS SKFRSLDLPKLEEVWSIAQEKPK+GDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYG+ EELK+CIEEFHSQDLLAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSP+GVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPSGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720

Query: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780
           SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY
Sbjct: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780

Query: 781 EHGNLCYNQDAHRQRIVDWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EHGNLCYNQDAHRQRIV+WINATGGTSSAFDVTTKGILHSALHNQYWR+IDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRMIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIRE+I
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREMI 900

Query: 901 NELIEARQRAEIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
           NELIEARQRA IHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDG+WQKF
Sbjct: 901 NELIEARQRAGIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGSWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLWLRQ
Sbjct: 961 VDKGSDYQLWLRQ 973

BLAST of Lsi07G010040 vs. NCBI nr
Match: KAG7036792.1 (Alpha-amylase 3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1773.8 bits (4593), Expect = 0.0e+00
Identity = 889/973 (91.37%), Postives = 921/973 (94.66%), Query Frame = 0

Query: 1   MGPFPLLDTAIEIFPRCPIITSGSSYGRRSSNCHHILRTVSATRNWKVSYIENLQSKRNT 60
           MGPFPLLD AIEI PRCPI+T GSSYGRRSSNCHH LRTVS T   KVSY +N   K  T
Sbjct: 1   MGPFPLLDAAIEILPRCPIVTPGSSYGRRSSNCHHNLRTVSVTWKRKVSYTDNFLHKPIT 60

Query: 61  VAFSSRDNSGDHLTDLVDDADGFSIGRSEVLETEEDEILAVKKALLESQTRQEAVEKERD 120
           VAFSSRDNS D            S GRSE+L +EEDEILAVKKALLESQTRQEAVEKE D
Sbjct: 61  VAFSSRDNSNDP-----------STGRSEILGSEEDEILAVKKALLESQTRQEAVEKETD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180
           QLLE+L RYEAKQKEY+ATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK
Sbjct: 121 QLLEKLTRYEAKQKEYLATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVETSYDIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAF+QATSHILEDAQ R+S AETSAVE SY+IEKQI DATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFEQATSHILEDAQLRISAAETSAVEASYEIEKQISDATEGSML 240

Query: 241 SFVEQSKIAIEKALDVAEKASAHAKKAVATFTDEVYPLDEIASIQSENIKLKGVVNEMES 300
           SFVEQSKIAIEKALDVAEKASAHA KAVATFTDEVYPLDEIASIQSE++KLKGVV+E+ES
Sbjct: 241 SFVEQSKIAIEKALDVAEKASAHANKAVATFTDEVYPLDEIASIQSESVKLKGVVDELES 300

Query: 301 HLSLARTDIDNLKLELEQARAQATASEIRAKNAEKALVEFQELSREKIIQQEGEIKLMME 360
           HLSLAR D+DNLKLELEQARAQATASEIRAKNAEKAL+EFQ  S EKIIQQEGEIKLMME
Sbjct: 301 HLSLARADVDNLKLELEQARAQATASEIRAKNAEKALLEFQNSSMEKIIQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIHAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKD  DKKKAASKAFKAELEGIKSAI AAKETAHSKDNAYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDFTDKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESFLLKERTMGKDNEVAAYIVNGGRIDLLTDDESQKWKLLSEGPRREIPQ 480
           KMWQQRA+MAESFL KERT+GKDNE AAY+VNGGRIDLLT+DESQKWKLLS+GPRREIPQ
Sbjct: 421 KMWQQRAEMAESFLSKERTLGKDNEEAAYVVNGGRIDLLTNDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDLTEISTSKFRSLDLPKLEEVWSIAQEKPKIGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKID++E+STSKFRSLDLPKLEEVWSIAQEKPK+GDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDISEVSTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           E+IEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ESIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELK+CIEEFHSQDLLAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKL WGPEAIVCDDPNFQGRGNP SGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLAWGPEAIVCDDPNFQGRGNPKSGDIFHAAPNIDH 720

Query: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780
           SQDFVR+DIKEWLNWLR+DIGFDGWRLDFVRGFSGTYVKEYIETSNP FAIGEYWDSLAY
Sbjct: 721 SQDFVRKDIKEWLNWLRSDIGFDGWRLDFVRGFSGTYVKEYIETSNPTFAIGEYWDSLAY 780

Query: 781 EHGNLCYNQDAHRQRIVDWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EHGNLCYNQDAHRQRIV+WINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKL QGYAYILTHPGTPT+FYDHFYDFGIREII
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLVQGYAYILTHPGTPTVFYDHFYDFGIREII 900

Query: 901 NELIEARQRAEIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
           NELIEARQRA IHCRSSVKI+HANNEGYVAQVGDTLVMKLGHFDWNPSKEN LDG WQKF
Sbjct: 901 NELIEARQRAGIHCRSSVKIFHANNEGYVAQVGDTLVMKLGHFDWNPSKENQLDGKWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLW+RQ
Sbjct: 961 VDKGSDYQLWMRQ 962

BLAST of Lsi07G010040 vs. NCBI nr
Match: XP_022948370.1 (uncharacterized protein LOC111452072 isoform X1 [Cucurbita moschata] >XP_022948371.1 uncharacterized protein LOC111452072 isoform X1 [Cucurbita moschata] >XP_022948373.1 uncharacterized protein LOC111452072 isoform X1 [Cucurbita moschata] >XP_022948374.1 uncharacterized protein LOC111452072 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1771.1 bits (4586), Expect = 0.0e+00
Identity = 888/973 (91.26%), Postives = 921/973 (94.66%), Query Frame = 0

Query: 1   MGPFPLLDTAIEIFPRCPIITSGSSYGRRSSNCHHILRTVSATRNWKVSYIENLQSKRNT 60
           MGPFPLLD AIEI PRCPI+T GSSYGRRSSNCHH LRTV+ T   KVSY +N   K  T
Sbjct: 1   MGPFPLLDAAIEILPRCPIVTPGSSYGRRSSNCHHNLRTVTVTWKRKVSYTDNFLHKPIT 60

Query: 61  VAFSSRDNSGDHLTDLVDDADGFSIGRSEVLETEEDEILAVKKALLESQTRQEAVEKERD 120
           VAFSSRD+S D            S GRSE+L +EEDEILAVKKALLESQTRQEAVEKE D
Sbjct: 61  VAFSSRDHSNDP-----------STGRSEILGSEEDEILAVKKALLESQTRQEAVEKETD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180
           QLLE+L RYEAKQKEY+ATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK
Sbjct: 121 QLLEKLTRYEAKQKEYLATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVETSYDIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAF+QATSHILEDAQ RVS AETSAVE SY+IEKQI DATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFEQATSHILEDAQLRVSAAETSAVEASYEIEKQISDATEGSML 240

Query: 241 SFVEQSKIAIEKALDVAEKASAHAKKAVATFTDEVYPLDEIASIQSENIKLKGVVNEMES 300
           SFVEQSKIAIEKALDVAEKASAHA KAVATFTDEVYPLDEIASIQSE++KLKGVV+E+ES
Sbjct: 241 SFVEQSKIAIEKALDVAEKASAHANKAVATFTDEVYPLDEIASIQSESVKLKGVVDELES 300

Query: 301 HLSLARTDIDNLKLELEQARAQATASEIRAKNAEKALVEFQELSREKIIQQEGEIKLMME 360
           HLSLAR D+DNLKLELEQARAQATASEIRAKNAEKAL+EFQ  S EKIIQQEGEIKLMME
Sbjct: 301 HLSLARADVDNLKLELEQARAQATASEIRAKNAEKALLEFQNSSMEKIIQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIHAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKD  DKKKAASKAFKAELEGIKSAI AAKETAHSKDNAYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDFTDKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESFLLKERTMGKDNEVAAYIVNGGRIDLLTDDESQKWKLLSEGPRREIPQ 480
           KMWQQRA+MAESFL KERT+GKDNE AAY+VNGGRIDLLT+DESQKWKLLS+GPRREIPQ
Sbjct: 421 KMWQQRAEMAESFLSKERTLGKDNEEAAYVVNGGRIDLLTNDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDLTEISTSKFRSLDLPKLEEVWSIAQEKPKIGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKID++E+STSKFRSLDLPKLEEVWSIAQEKPK+GDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDISEVSTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           E+IEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ESIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELK+CIEEFHSQDLLAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKL WGPEAIVCDDPNFQGRGNP SGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLAWGPEAIVCDDPNFQGRGNPKSGDIFHAAPNIDH 720

Query: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780
           SQDFVR+DIKEWLNWLR+DIGFDGWRLDFVRGFSGTYVKEYIETSNP FAIGEYWDSLAY
Sbjct: 721 SQDFVRKDIKEWLNWLRSDIGFDGWRLDFVRGFSGTYVKEYIETSNPTFAIGEYWDSLAY 780

Query: 781 EHGNLCYNQDAHRQRIVDWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EHGNLCYNQDAHRQRIV+WINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKL QGYAYILTHPGTPT+FYDHFYDFGIREII
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLVQGYAYILTHPGTPTVFYDHFYDFGIREII 900

Query: 901 NELIEARQRAEIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
           NELIEARQRA IHCRSSVKI+HANNEGYVAQVGDTLVMKLGHFDWNPSKEN LDG WQKF
Sbjct: 901 NELIEARQRAGIHCRSSVKIFHANNEGYVAQVGDTLVMKLGHFDWNPSKENQLDGKWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLW+RQ
Sbjct: 961 VDKGSDYQLWMRQ 962

BLAST of Lsi07G010040 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 548.1 bits (1411), Expect = 1.4e-155
Identity = 262/488 (53.69%), Postives = 336/488 (68.85%), Query Frame = 0

Query: 494 PPRKIDLTEISTSKFRSLDLPKLE----EVWSIAQEKPKIGDTLIEHVIEKETIEKKRKA 553
           P RK D  E+S S F    + ++     ++ S   +K  + +     + E E +  +  +
Sbjct: 403 PKRKTD-KEVSASGFTKEIITEIRNLAIDISSHKNQKTNVKEVQENILQEIEKLAAEAYS 462

Query: 554 LERAL-----QRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRR-RWYLELAAKAS 613
           + R+      +   ++ +      K+  GTG+G EI+ QGFNWES +  RWYLEL  KA 
Sbjct: 463 IFRSTTPAFSEEGVLEAEADKPDIKISSGTGSGFEILCQGFNWESNKSGRWYLELQEKAD 522

Query: 614 DLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLALGDV 673
           +L+  G T +WLPPPTESV+P+GYMP DLYNLNS YG+ +ELK  +++FH   +  LGD 
Sbjct: 523 ELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDA 582

Query: 674 VLNHRCAHKQSPNGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDHSQD 733
           VLNHRCAH ++ NGVWN+FGG+L W   A+V DDP+FQGRGN SSGD FHAAPNIDHSQD
Sbjct: 583 VLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDPHFQGRGNKSSGDNFHAAPNIDHSQD 642

Query: 734 FVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAYEHG 793
           FVR+DIKEWL W+  ++G+DGWRLDFVRGF G YVK+Y++ S P FA+GEYWDSL+Y +G
Sbjct: 643 FVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYG 702

Query: 794 NLCYNQDAHRQRIVDWINATGGTSSAFDVTTKGILHSALHN-QYWRLIDPQGKPTGVVGW 853
            + YNQDAHRQRIVDWINAT G + AFDVTTKGILH+AL   +YWRL DP+GKP GVVGW
Sbjct: 703 EMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGW 762

Query: 854 WPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREIINE 913
           WPSRAVTF+ENHDTGSTQGHW FP  K  QGYAYILTHPGTP +F+DH +     EI   
Sbjct: 763 WPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAVFFDHIFSDYHSEIA-A 822

Query: 914 LIEARQRAEIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKFVD 971
           L+  R R ++HCRS V I  +  + Y A + + + MK+G   + P   +    NW   V+
Sbjct: 823 LLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQ---NWSVAVE 882

BLAST of Lsi07G010040 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 444.5 bits (1142), Expect = 2.2e-124
Identity = 206/409 (50.37%), Postives = 268/409 (65.53%), Query Frame = 0

Query: 566 DQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAAKASDLSQSGITAVWLPPPTESVAPQ 625
           DQT +      G E++ Q +NWES +  W+  L  K  D+++SG T+ WLPPP++S+AP+
Sbjct: 13  DQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPE 72

Query: 626 GYMPSDLYNLNSSYGSEEELKFCIEEFHSQDLLALGDVVLNHRCAHKQSPNGVWNIFGG- 685
           GY+P DLY+LNS+YGSE  LK  + +     + A+ D+V+NHR    +   G++N + G 
Sbjct: 73  GYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGI 132

Query: 686 KLTWGPEAIV-CDDPNFQGRGNPSSGDIFHAAPNIDHSQDFVRRDIKEWLNWLRNDIGFD 745
            L W   A+  C      G GN S+GD F+  PN+DH+Q FVR+DI  WL WLRN +GF 
Sbjct: 133 SLPWDEHAVTSCTG----GLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQ 192

Query: 746 GWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAYEHGNLCYNQDAHRQRIVDWINAT 805
            +R DF RG+S  YVKEYI  + P F++GE WDS  Y    L YNQD+HRQRI+ WI+AT
Sbjct: 193 DFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDAT 252

Query: 806 GGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTGSTQGHW 865
           G  S+AFD TTKGIL  A+  QYWRL D QGKP GV+GWWPSRAVTFL+NHDTGSTQ HW
Sbjct: 253 GQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHW 312

Query: 866 PFPRDKLAQGYAYILTHPGTPTIFYDHFYDFG--IREIINELIEARQRAEIHCRSSVKIY 925
           PFP   + +GYAYILTHPG P +FYDHFYD+G  I + I +LI+ R+R +IH RS+V++ 
Sbjct: 313 PFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVL 372

Query: 926 HANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKFVDKGSDYQLW 971
            A +  Y A VG+ + MKLG   W PS      G        G  Y +W
Sbjct: 373 KAESNLYAAIVGEKICMKLGDGSWCPS------GRDWTLATSGHRYAVW 411

BLAST of Lsi07G010040 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 365.9 bits (938), Expect = 9.9e-101
Identity = 171/372 (45.97%), Postives = 243/372 (65.32%), Query Frame = 0

Query: 580 IVFQGFNWESWRRR--WYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNS 639
           ++FQ FNWESW++   +Y  L     D++ +GIT +WLPPP++SVAP+GY+P  LY+LNS
Sbjct: 27  LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86

Query: 640 S-YGSEEELKFCIEEFHSQDLLALGDVVLNHRCAHKQSPNGVWNIFGG-----KLTWGPE 699
           S YGSE ELK  I+  + + + AL D+V+NHR A ++     +  F G     +L W P 
Sbjct: 87  SKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPS 146

Query: 700 AIVCDDPNFQGRGNPSSGDIFHAAPNIDHSQDFVRRDIKEWLNWLRNDIGFDGWRLDFVR 759
            +  +DP F G GN  +G  F  AP+IDH    V++++ EW+NWL+ +IGF GWR D+VR
Sbjct: 147 FVCRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVR 206

Query: 760 GFSGTYVKEYIETSNPAFAIGEYWDSLAY-EHGNLCYNQDAHRQRIVDWI-NATGGTSSA 819
           G++ +  K Y++ ++P FA+GE WD + Y   G L Y+Q+ HR  +  WI  A GG  +A
Sbjct: 207 GYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTA 266

Query: 820 FDVTTKGILHSALHNQYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTGSTQGHWPFPRDK 879
           FD TTKGIL SA+  + WRL D QGKP G++G  P  AVTF++NHDT  T   W FP DK
Sbjct: 267 FDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSDK 326

Query: 880 LAQGYAYILTHPGTPTIFYDHFYDFGIREIINELIEARQRAEIHCRSSVKIYHANNEGYV 939
           +  GY YILTHPGTP IFY+H+ ++G++E I++L+  R +  I   SSV I  A  + Y+
Sbjct: 327 VLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVTIKAAEADLYL 386

Query: 940 AQVGDTLVMKLG 942
           A + D ++MK+G
Sbjct: 387 AMIDDKVIMKIG 395

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94A412.0e-15453.69Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1[more]
Q8LFG13.1e-12350.37Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1[more]
A2YGY21.1e-11552.08Alpha-amylase isozyme 2A OS=Oryza sativa subsp. indica OX=39946 GN=AMYC2 PE=2 SV... [more]
Q0D9J11.1e-11552.08Alpha-amylase isozyme 2A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY2A PE=2 ... [more]
P006934.4e-11448.95Alpha-amylase type A isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3C1E00.0e+0095.271,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo OX=3656 GN=LOC103495777 PE=4... [more]
A0A6J1G9550.0e+0091.261,4-alpha-D-glucan glucanohydrolase OS=Cucurbita moschata OX=3662 GN=LOC11145207... [more]
A0A6J1KAV60.0e+0090.851,4-alpha-D-glucan glucanohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111492635 ... [more]
A0A6J1DP260.0e+0091.17uncharacterized protein LOC111022188 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1G9N40.0e+0090.96uncharacterized protein LOC111452072 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
XP_038893540.10.0e+0095.79uncharacterized protein LOC120082441 [Benincasa hispida][more]
XP_008455663.10.0e+0095.27PREDICTED: uncharacterized protein LOC103495777 [Cucumis melo] >XP_008455664.1 P... [more]
XP_004137176.10.0e+0095.17uncharacterized protein LOC101217339 [Cucumis sativus] >KAE8649296.1 hypothetica... [more]
KAG7036792.10.0e+0091.37Alpha-amylase 3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022948370.10.0e+0091.26uncharacterized protein LOC111452072 isoform X1 [Cucurbita moschata] >XP_0229483... [more]
Match NameE-valueIdentityDescription
AT1G69830.11.4e-15553.69alpha-amylase-like 3 [more]
AT1G76130.12.2e-12450.37alpha-amylase-like 2 [more]
AT4G25000.19.9e-10145.97alpha-amylase-like [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 295..336
NoneNo IPR availableCOILSCoilCoilcoord: 105..139
NoneNo IPR availableCOILSCoilCoilcoord: 533..560
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 579..911
e-value: 1.6E-131
score: 440.8
NoneNo IPR availablePANTHERPTHR43447ALPHA-AMYLASEcoord: 126..973
NoneNo IPR availablePANTHERPTHR43447:SF30ALPHA AMYLASE DOMAIN PROTEINcoord: 126..973
NoneNo IPR availableCDDcd11314AmyAc_arch_bac_plant_AmyAcoord: 580..918
e-value: 7.94484E-176
score: 510.996
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 913..971
IPR012850Alpha-amylase, C-terminal beta-sheetSMARTSM00810alpha-amyl_c2coord: 908..972
e-value: 6.1E-6
score: 35.8
IPR012850Alpha-amylase, C-terminal beta-sheetPFAMPF07821Alpha-amyl_C2coord: 909..970
e-value: 8.6E-17
score: 61.2
IPR006047Glycosyl hydrolase, family 13, catalytic domainSMARTSM00642aamycoord: 579..907
e-value: 2.2E-37
score: 140.2
IPR006047Glycosyl hydrolase, family 13, catalytic domainPFAMPF00128Alpha-amylasecoord: 607..669
e-value: 2.5E-8
score: 33.9
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 912..972
e-value: 2.3E-11
score: 45.6
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 584..896

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G010040.1Lsi07G010040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0004556 alpha-amylase activity
molecular_function GO:0103025 alpha-amylase activity (releasing maltohexaose)
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0003824 catalytic activity