Lsi07G009310 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G009310
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionnon-specific lipid-transfer protein 2-like
Locationchr07: 11322357 .. 11322650 (-)
RNA-Seq ExpressionLsi07G009310
SyntenyLsi07G009310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTTATTGTCAATAACAAGTCTCTGTGTTTTGGTGGCGGTGGCGGCGACGATGGTGCTTCTGACTGGAGCTCGTTTGGCCGATGCGGCGGACTGCAATCCTTCGGAGCTGAGCCCATGTCTTGATGCAATTAGGTCGTCGTCGGTGGCACCGTCGAGCAGTTGCTGCGTTAAGATGGAAGAGCAGGGGCCGTGCCTCTGCCAATACGCTAAGATTCCAAAGTTGGAGCCTTTCGTTGCCGGCGGGCAACGGGTTGCCGCTGCTTGCGGCGTCACGCTTCCTGTGTGTTAG

mRNA sequence

ATGAAGTTATTGTCAATAACAAGTCTCTGTGTTTTGGTGGCGGTGGCGGCGACGATGGTGCTTCTGACTGGAGCTCGTTTGGCCGATGCGGCGGACTGCAATCCTTCGGAGCTGAGCCCATGTCTTGATGCAATTAGGTCGTCGTCGGTGGCACCGTCGAGCAGTTGCTGCGTTAAGATGGAAGAGCAGGGGCCGTGCCTCTGCCAATACGCTAAGATTCCAAAGTTGGAGCCTTTCGTTGCCGGCGGGCAACGGGTTGCCGCTGCTTGCGGCGTCACGCTTCCTGTGTGTTAG

Coding sequence (CDS)

ATGAAGTTATTGTCAATAACAAGTCTCTGTGTTTTGGTGGCGGTGGCGGCGACGATGGTGCTTCTGACTGGAGCTCGTTTGGCCGATGCGGCGGACTGCAATCCTTCGGAGCTGAGCCCATGTCTTGATGCAATTAGGTCGTCGTCGGTGGCACCGTCGAGCAGTTGCTGCGTTAAGATGGAAGAGCAGGGGCCGTGCCTCTGCCAATACGCTAAGATTCCAAAGTTGGAGCCTTTCGTTGCCGGCGGGCAACGGGTTGCCGCTGCTTGCGGCGTCACGCTTCCTGTGTGTTAG

Protein sequence

MKLLSITSLCVLVAVAATMVLLTGARLADAADCNPSELSPCLDAIRSSSVAPSSSCCVKMEEQGPCLCQYAKIPKLEPFVAGGQRVAAACGVTLPVC
Homology
BLAST of Lsi07G009310 vs. ExPASy Swiss-Prot
Match: Q43681 (Probable non-specific lipid-transfer protein AKCS9 OS=Vigna unguiculata OX=3917 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 9.2e-12
Identity = 41/96 (42.71%), Postives = 60/96 (62.50%), Query Frame = 0

Query: 4  LSITSLCVLVAVAATMVLLTGARLADAADCNPSELSPCLDAIRSSSVAPSSSCCVKMEEQ 63
          + ++ +C +V VA  + L+    +A+A  CNP+ELS C+ AI   S  PSS+CC K++ Q
Sbjct: 7  MKMSVVCAVVVVA--LFLIDVGPVAEAVTCNPTELSSCVPAITGGS-KPSSTCCSKLKVQ 66

Query: 64 GPCLCQYAKIPKLEPFV--AGGQRVAAACGVTLPVC 98
           PCLC Y K P L+ +V   G ++V + CGVT P C
Sbjct: 67 EPCLCNYIKNPSLKQYVNSPGAKKVLSNCGVTYPNC 99

BLAST of Lsi07G009310 vs. ExPASy Swiss-Prot
Match: P82353 (Non-specific lipid-transfer protein 2 OS=Prunus armeniaca OX=36596 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 3.3e-09
Identity = 30/67 (44.78%), Postives = 43/67 (64.18%), Query Frame = 0

Query: 33 CNPSELSPCLDAIRSSSVAPSSSCCVKMEEQGPCLCQYAKIPKLEPFV--AGGQRVAAAC 92
          C+P +LSPCL  I S + +P ++CC K+ EQ PCLC Y K P L  +V     +++A+ C
Sbjct: 3  CSPVQLSPCLGPINSGAPSP-TTCCQKLREQRPCLCGYLKNPSLRQYVNSPNARKLASNC 62

Query: 93 GVTLPVC 98
          GV +P C
Sbjct: 63 GVPVPQC 68

BLAST of Lsi07G009310 vs. ExPASy TrEMBL
Match: A0A0A0L0Z5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G135200 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 5.4e-31
Identity = 72/97 (74.23%), Postives = 79/97 (81.44%), Query Frame = 0

Query: 1  MKLLSITSLCVLVAVAATMVLLTGARLADAADCNPSELSPCLDAIRSSSVAPSSSCCVKM 60
          M  LSITSLCVLVAV   M LLTGAR  DA +C P EL+PC+DAI+SSSV PSS CC+KM
Sbjct: 1  MMRLSITSLCVLVAVVEMMALLTGARSVDAVECIPQELTPCIDAIKSSSVVPSSICCMKM 60

Query: 61 EEQGPCLCQYAKIPKLEPFVAGGQRVAAACGVTLPVC 98
          EEQ PCLCQYAKIP  +P +AGGQRVAA CGVTLPVC
Sbjct: 61 EEQAPCLCQYAKIPSFKPLIAGGQRVAAVCGVTLPVC 97

BLAST of Lsi07G009310 vs. ExPASy TrEMBL
Match: A0A6J1GV37 (non-specific lipid-transfer protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111457740 PE=4 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.8e-26
Identity = 68/97 (70.10%), Postives = 78/97 (80.41%), Query Frame = 0

Query: 1  MKLLSITSLCVLVAVAATMVLLTGARLADAADCNPSELSPCLDAIRSSSVAPSSSCCVKM 60
          MK LSI+SLCVLVA  ATM LLTGA +ADA DCNPSELSPCL AI +SS  P+SSCC KM
Sbjct: 1  MKRLSISSLCVLVAAVATMALLTGAPVADAVDCNPSELSPCLPAI-TSSTPPTSSCCQKM 60

Query: 61 EEQGPCLCQYAKIPKLEPFVAGGQRVAAACGVTLPVC 98
           EQ PCLC Y K P+ +P+V GG+RVAAACG+T+P C
Sbjct: 61 VEQTPCLCGYFKNPEFKPYVTGGKRVAAACGLTVPEC 96

BLAST of Lsi07G009310 vs. ExPASy TrEMBL
Match: A0A5A7VA95 (Non-specific lipid-transfer protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3068G00070 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 7.5e-25
Identity = 57/79 (72.15%), Postives = 66/79 (83.54%), Query Frame = 0

Query: 19 MVLLTGARLADAADCNPSELSPCLDAIRSSSVAPSSSCCVKMEEQGPCLCQYAKIPKLEP 78
          M LL+GA   +A +C P EL+PC+DAI+SSSVAPSS CC+KMEEQ PCLCQYAKIP  +P
Sbjct: 1  MALLSGAPSVNAVECIPQELTPCIDAIKSSSVAPSSICCMKMEEQAPCLCQYAKIPSFKP 60

Query: 79 FVAGGQRVAAACGVTLPVC 98
           +AGGQRVAA CGVTLPVC
Sbjct: 61 LIAGGQRVAAVCGVTLPVC 79

BLAST of Lsi07G009310 vs. ExPASy TrEMBL
Match: A0A6J1E135 (non-specific lipid-transfer protein 2-like OS=Momordica charantia OX=3673 GN=LOC111024980 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.1e-15
Identity = 51/99 (51.52%), Postives = 70/99 (70.71%), Query Frame = 0

Query: 1  MKLLSITSLCVLVAVAATMVLLTGARLADAADCNPSELSPCLDAIRSSSVAPSSSCCVKM 60
          MK   + SLC+LVAV AT+ LL+GAR  +AA CNP ELSPCL AI +SS  PS+ CC K+
Sbjct: 1  MKKFQVPSLCLLVAVVATVALLSGARSVEAA-CNPMELSPCLGAI-TSSAPPSTVCCQKL 60

Query: 61 EEQGPCLCQYAKIPKLEPFVAG--GQRVAAACGVTLPVC 98
          ++Q PC C+YAK P+++P+V     + VA+ C + +P C
Sbjct: 61 KQQQPCFCEYAKDPRMKPYVESPRAKAVASICRIAVPRC 97

BLAST of Lsi07G009310 vs. ExPASy TrEMBL
Match: A0A6J1CQB8 (non-specific lipid-transfer protein 2-like OS=Momordica charantia OX=3673 GN=LOC111013188 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.2e-14
Identity = 54/99 (54.55%), Postives = 64/99 (64.65%), Query Frame = 0

Query: 1  MKLLSITSLCVLVAVAATMVLLTGARLADAADCNPSELSPCLDAIRSSSVAPSSSCCVKM 60
          MK L IT L +L  V A M LLTGA +A+A  C+P +LS C  AI SSS  PS+SCC K+
Sbjct: 1  MKTLPITGLWLL--VVAIMALLTGAHVAEAVTCSPLQLSSCAGAI-SSSTRPSASCCSKL 60

Query: 61 EEQGPCLCQYAKIPKLEPFV--AGGQRVAAACGVTLPVC 98
           EQ PCLC Y K P L P+V   G + VA+ACGV  P C
Sbjct: 61 REQRPCLCGYLKNPNLRPYVQSPGAKTVASACGVAFPSC 96

BLAST of Lsi07G009310 vs. NCBI nr
Match: KAG6581743.1 (hypothetical protein SDJN03_21745, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 128.3 bits (321), Expect = 3.7e-26
Identity = 68/97 (70.10%), Postives = 78/97 (80.41%), Query Frame = 0

Query: 1  MKLLSITSLCVLVAVAATMVLLTGARLADAADCNPSELSPCLDAIRSSSVAPSSSCCVKM 60
          MK LSI+SLCVLVA A TM LLTGA +ADA DCNPSELSPCL AI +SS  P+SSCC KM
Sbjct: 1  MKRLSISSLCVLVAAAVTMALLTGAPVADAVDCNPSELSPCLPAI-TSSTPPTSSCCQKM 60

Query: 61 EEQGPCLCQYAKIPKLEPFVAGGQRVAAACGVTLPVC 98
           EQ PCLC Y K P+ +P+V GG+RVAAACG+T+P C
Sbjct: 61 VEQTPCLCGYFKNPEFKPYVTGGKRVAAACGLTVPEC 96

BLAST of Lsi07G009310 vs. NCBI nr
Match: XP_022955891.1 (non-specific lipid-transfer protein 2-like [Cucurbita moschata])

HSP 1 Score: 128.3 bits (321), Expect = 3.7e-26
Identity = 68/97 (70.10%), Postives = 78/97 (80.41%), Query Frame = 0

Query: 1  MKLLSITSLCVLVAVAATMVLLTGARLADAADCNPSELSPCLDAIRSSSVAPSSSCCVKM 60
          MK LSI+SLCVLVA  ATM LLTGA +ADA DCNPSELSPCL AI +SS  P+SSCC KM
Sbjct: 1  MKRLSISSLCVLVAAVATMALLTGAPVADAVDCNPSELSPCLPAI-TSSTPPTSSCCQKM 60

Query: 61 EEQGPCLCQYAKIPKLEPFVAGGQRVAAACGVTLPVC 98
           EQ PCLC Y K P+ +P+V GG+RVAAACG+T+P C
Sbjct: 61 VEQTPCLCGYFKNPEFKPYVTGGKRVAAACGLTVPEC 96

BLAST of Lsi07G009310 vs. NCBI nr
Match: XP_023527040.1 (non-specific lipid-transfer protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 125.2 bits (313), Expect = 3.1e-25
Identity = 66/97 (68.04%), Postives = 77/97 (79.38%), Query Frame = 0

Query: 1  MKLLSITSLCVLVAVAATMVLLTGARLADAADCNPSELSPCLDAIRSSSVAPSSSCCVKM 60
          MK LSI+SLCVLV   A+M LLTGA +ADA DCNPSELSPCL AI +SS  P+SSCC KM
Sbjct: 1  MKRLSISSLCVLVVAVASMALLTGAPVADAVDCNPSELSPCLPAI-TSSTPPTSSCCQKM 60

Query: 61 EEQGPCLCQYAKIPKLEPFVAGGQRVAAACGVTLPVC 98
           EQ PCLC Y K P+ +P+V GG+RVAAACG+T+P C
Sbjct: 61 VEQTPCLCGYFKNPEFKPYVTGGKRVAAACGLTVPEC 96

BLAST of Lsi07G009310 vs. NCBI nr
Match: KAA0064150.1 (non-specific lipid-transfer protein 2-like [Cucumis melo var. makuwa] >TYK16011.1 non-specific lipid-transfer protein 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 122.9 bits (307), Expect = 1.6e-24
Identity = 57/79 (72.15%), Postives = 66/79 (83.54%), Query Frame = 0

Query: 19 MVLLTGARLADAADCNPSELSPCLDAIRSSSVAPSSSCCVKMEEQGPCLCQYAKIPKLEP 78
          M LL+GA   +A +C P EL+PC+DAI+SSSVAPSS CC+KMEEQ PCLCQYAKIP  +P
Sbjct: 1  MALLSGAPSVNAVECIPQELTPCIDAIKSSSVAPSSICCMKMEEQAPCLCQYAKIPSFKP 60

Query: 79 FVAGGQRVAAACGVTLPVC 98
           +AGGQRVAA CGVTLPVC
Sbjct: 61 LIAGGQRVAAVCGVTLPVC 79

BLAST of Lsi07G009310 vs. NCBI nr
Match: KAE8648023.1 (hypothetical protein Csa_005844, partial [Cucumis sativus])

HSP 1 Score: 107.8 bits (268), Expect = 5.2e-20
Identity = 48/64 (75.00%), Postives = 54/64 (84.38%), Query Frame = 0

Query: 34 NPSELSPCLDAIRSSSVAPSSSCCVKMEEQGPCLCQYAKIPKLEPFVAGGQRVAAACGVT 93
          +PS   PC+DAI+SSSV PSS CC+KMEEQ PCLCQYAKIP  +P +AGGQRVAA CGVT
Sbjct: 33 HPSGADPCIDAIKSSSVVPSSICCMKMEEQAPCLCQYAKIPSFKPLIAGGQRVAAVCGVT 92

Query: 94 LPVC 98
          LPVC
Sbjct: 93 LPVC 96

BLAST of Lsi07G009310 vs. TAIR 10
Match: AT1G73780.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 7.5e-09
Identity = 30/74 (40.54%), Postives = 41/74 (55.41%), Query Frame = 0

Query: 26 RLADAADCNPSELSPCLDAIRSSSVAPSSSCCVKMEEQGPCLCQYAKIPKLEPFV--AGG 85
          R+A    CNP++LSPCL+ I   S  PS  CC K++EQ  C+CQY K P  + F+     
Sbjct: 26 RVAAVDPCNPAQLSPCLETIMKGS-EPSDLCCSKVKEQQHCICQYLKNPNFKSFLNSPNA 85

Query: 86 QRVAAACGVTLPVC 98
          + +A  C    P C
Sbjct: 86 KIIATDCHCPYPKC 98

BLAST of Lsi07G009310 vs. TAIR 10
Match: AT1G07747.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 1.3e-08
Identity = 29/86 (33.72%), Postives = 47/86 (54.65%), Query Frame = 0

Query: 12 LVAVAATMVLLTGARLADAADCNPSELSPCLDAIRSSSVAPSSSCCVKMEEQGPCLCQYA 71
          LV VA  +  +   +  + A C  ++L  CL  I      PS+ CC K++++  C C+Y 
Sbjct: 8  LVFVAFVLSSMAPTKAEEKAACVVTDLKACLPDI-IGETPPSTKCCTKLKDKKSCFCEYL 67

Query: 72 KIPKLEPFVAGGQRVAAACGVTLPVC 98
          K P + P++   ++V  ACGV +PVC
Sbjct: 68 KNPLIAPYMTSAKQVLEACGVPVPVC 92

BLAST of Lsi07G009310 vs. TAIR 10
Match: AT1G43667.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 1.7e-08
Identity = 23/65 (35.38%), Postives = 37/65 (56.92%), Query Frame = 0

Query: 33 CNPSELSPCLDAIRSSSVAPSSSCCVKMEEQGPCLCQYAKIPKLEPFVAGGQRVAAACGV 92
          C  +EL PCL ++   S  PS+ CC K++EQ  C C Y + P+   ++   +++ AAC +
Sbjct: 35 CTVTELQPCLPSVIDGS-QPSTQCCEKLKEQNSCFCDYLQNPQFSQYITAAKQILAACKI 94

Query: 93 TLPVC 98
            P C
Sbjct: 95 PYPNC 98

BLAST of Lsi07G009310 vs. TAIR 10
Match: AT5G38170.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 54.3 bits (129), Expect = 6.3e-08
Identity = 34/87 (39.08%), Postives = 48/87 (55.17%), Query Frame = 0

Query: 13  VAVAATMVLLTGARLADAADCNPSELSPCLDAIRSSSVAPSSSCCVKMEEQGPCLCQYAK 72
           V++ AT V L+G   AD   C+  +LS C   +  + V PS+ CC K++EQ PC C Y K
Sbjct: 20  VSIRATEVKLSGGE-ADVT-CDAVQLSSCATPM-LTGVPPSTECCGKLKEQQPCFCTYIK 79

Query: 73  IPKLEPFV--AGGQRVAAACGVTLPVC 98
            P+   +V  A  ++  A CGV  P C
Sbjct: 80  DPRYSQYVGSANAKKTLATCGVPYPTC 103

BLAST of Lsi07G009310 vs. TAIR 10
Match: AT1G66850.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 3.5e-06
Identity = 32/103 (31.07%), Postives = 48/103 (46.60%), Query Frame = 0

Query: 4   LSITSLCVLVAVAATMVLLTGARLADAAD-------CNPSELSPCLDAIRSSSVAPSSSC 63
           + I +L ++V V  +       +  D  D       C+  +L PCL AI      PS +C
Sbjct: 1   MKIVTLVLVVFVILSTSFPAAIKAEDTGDTGNVGVTCDARQLQPCLAAITGGG-QPSGAC 60

Query: 64  CVKMEEQGPCLCQYAKIPKLEPFVA--GGQRVAAACGVTLPVC 98
           C K+ EQ  CLC +AK P    +++    ++V  AC V  P C
Sbjct: 61  CAKLTEQQSCLCGFAKNPAFAQYISSPNARKVLLACNVAYPTC 102

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q436819.2e-1242.71Probable non-specific lipid-transfer protein AKCS9 OS=Vigna unguiculata OX=3917 ... [more]
P823533.3e-0944.78Non-specific lipid-transfer protein 2 OS=Prunus armeniaca OX=36596 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L0Z55.4e-3174.23Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G135200 PE=4 SV=1[more]
A0A6J1GV371.8e-2670.10non-specific lipid-transfer protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A5A7VA957.5e-2572.15Non-specific lipid-transfer protein 2-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A6J1E1351.1e-1551.52non-specific lipid-transfer protein 2-like OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1CQB81.2e-1454.55non-specific lipid-transfer protein 2-like OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
KAG6581743.13.7e-2670.10hypothetical protein SDJN03_21745, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022955891.13.7e-2670.10non-specific lipid-transfer protein 2-like [Cucurbita moschata][more]
XP_023527040.13.1e-2568.04non-specific lipid-transfer protein 2-like [Cucurbita pepo subsp. pepo][more]
KAA0064150.11.6e-2472.15non-specific lipid-transfer protein 2-like [Cucumis melo var. makuwa] >TYK16011.... [more]
KAE8648023.15.2e-2075.00hypothetical protein Csa_005844, partial [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G73780.17.5e-0940.54Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT1G07747.11.3e-0833.72Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT1G43667.11.7e-0835.38Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT5G38170.16.3e-0839.08Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT1G66850.13.5e-0631.07Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilyGENE3D1.10.110.10coord: 31..97
e-value: 1.5E-17
score: 65.6
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilySUPERFAMILY47699Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumincoord: 33..97
NoneNo IPR availablePANTHERPTHR33214:SF44LIPID TRANSFER PROTEIN FAMILY PROTEIN-RELATEDcoord: 7..97
IPR033872Non-specific lipid-transfer protein type 2PANTHERPTHR33214BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEINcoord: 7..97

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G009310.1Lsi07G009310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006869 lipid transport