Lsi07G008040 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G008040
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionSerine hydroxymethyltransferase
Locationchr07: 8927416 .. 8931789 (+)
RNA-Seq ExpressionLsi07G008040
SyntenyLsi07G008040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAAAACCCTAAGACCCAAAATGTCTCCTCGTCCCCCTCTTTCACGTTAAGAGCAGCCTCCTCTCCTCCTCTTCTTTCTTTCTGTCTCCCTCTCTTTGCCGTGAAACTCCAGCCGGCAGCCACCCAACCAGCATCATCTTCATCCTTTGCAAGCTTCAATCCACGGGCGACGACGACAAAAACTCCACGGCGGCGTCCCGCGACTTGGAGCAGATAGCGACCCACGCATTCTCTCTGCCTCCGACAGCGCCTGTACGCGACGTCAAGTCCAGCTCCGACGAGTGGCAGCCGAAAACCAATCCGTTGCTGCTCGTTTTGGCTAACCCACGACGTCTGTGACCTCCGAAAACCTTGTAGCAGCAACTTGCGCGACACAAACACCCTGACAACAATCTTTTCCTCTTTTCGGTGGGTAAGAATGTTCTAAATTCCTCTTTGAACTAGTTTAAGGTTCTTTAGATTGGTCTCTAACACTTGGAAGCCCTTATTGGTAGATTCGGAACGATTCAGACACTTTGCAGCAAGGACCCAAGTCTCTTTTGGTGAGTTTTAGGCTTTAGAACTCCGTTTAGATCCTTTCTACTTCAATTAGATGCTGTTAATAAGGTCTTAACTCTCGGGCACTCTCTGTTTGTAGTTTAGACTTGTTCTGAAGTGTTCCGACTGAGATTAAGATTATTTGAGTTTGCGAATGAGGTTTCAAACTAAGTTCTAACCTTGGAACTTGAATATTATTATTTTCGAACTAAGTTCTCCGTTGAAGTATTGTTATGTTGGCTATAACTGATTAATAGTTTTTGTTTTCAGCTGATAAGAATTTCAGAGCGTCGAAGTGTTTTGTGGGTCATATAGAAAATAGCTATGTGCCCAAATACGCTACTAGACTATGTTGCATGTTGTTTTTGAACTTTTGATGTTTGGGTGAAGTTATTGGCGTTTGGTTGTTACTGAAGTTCGTTTTGTTGTATGAAATTTGTAGGGATTGTGAAGATTTAAGTGAGAATGCAGGCTACAAGTGGTGCTGCAGTGATGGGCTCTCTGCAAGTTGCTGTTTGTGCGAAGGGATCATGTTTTCCTTCAAAAGGTTCTAGCATCTGTGTGTTTCCACAACACAAGAAAATGAACATCCTGAAACCCTATAGATCCTTTAATGTTGAAGCAAGCATGGTTGCGGGGAAGCCATCATCTTCTGTATCGGTCACTGTTCCAGAAATTGGAGGTGAAATATTATCCAACTCCATTCACCGATGTTATGATTTGTTTGAAAGAAATTTGGCATATCTGCTTGACCCATGTTAATAGAATGGTCGAATATTTGACCCACTACTCGGAGGAAAAAAAGTGTATAGTATTTATTTTGTTTCTTAATATTAGATTAGCCAAGTTGGTTCCTATGAATGAAAAGAATTTAAGTTTGATTGAGTGGGAGAATTTAGCTTAGGTTACCTTCATTGCTGTCATGCAGGTGTAAGCCAGTTTGTAGATCATGCCTTGAGTGAAATAGATCCCGAAGTTCGAAGTATTATTAACAAAGAAAAGCAGCGACAATTTAAAAGCCTTGAACTTATTGCCTCGGAGAATTTTACATCTCGGGCAGTTATGGAGGCAGTAGGGTCTTGTCTCACAAACAAATATTCAGAAGGATTACCTGGAAAAAGGTAAAATTTCTTCATTTGATGTACTATGGATGTGAATTCCAATTCTTTCCGGGGAGGTTTTGAGATGTTTTTGTTGTTGAAAGGATTATGCAAATTAAGATTTTTATCATGCATCTCAGGTATTATGGTGGCAATGAACATATTGACGAGCTTGAAACACTCTGTCAACAAAGGGCTCTCGAAGCATTTCATTTGGACAATAACAAGTGGGGTGTAAATGTTCAACCGTTATCTGGTTCACCTGCTAACTTCGAGGTTTATACTGCAATTCTTAATCCACATGATCGGATCATGGTGAGTAGCCTTATGATTACTTCTTTAACCACCGTGTTAATTTAGACAATTGCTGATGCTACTGATTCATGTTAATTGATGTTAGGGTTTAGACTTGCCTCATGGAGGACATTTATCTCATGGGTTTATGACACCTAAAAGGCGGGTGTCAGGCGCATCAATTTATTTTGAATCCATGCCTTATCGTCTTGATGAGACTACAGGTACTTCCCATGTGTGATACAGTTTTTTTATGGTCTCTTGCATGGTTTATGCATTCTAATTTAGTAGCAAACTCTTGCAGGCATCGTAGATTATGACATGCTCGAAAAAACTGCTACCTTGTTTCGACCAAAGCTGATTATTGCTGGTGCAAGTGCTTATCCACGAGATTTTGACTATCCTCGCATGAGAAAAGTGAGGATTTACCTAAATCCAATGTTGTTCATCATTTTACATTGAGTAGCTCTATTTAGAAAATGAGTAAGCATGAATGACATACTTGATTTGATATGGGTAGATTGCCGATGCTGTTGGTGCTTTTCTCATGATGGATATGGCTCACATAAGTGGGCTTGTTGCTGCTTCTGTGGTTGCTAATCCTTTTGAATACTGTGATATTGTGACCACGACAACACACAAGGTAAAGGATAAATTTTGCTAATGAATGCTTCCTTTTCAGTACAGGCTTTCCTCTAAATTAATTTCATTTTTTGAGATAAGAAATGGGTATATTCAAGAACCAAATGGGAAAGAGGAACAACCTATCGGCCAAGGTAGAGAAAACTAATACCAAAAAAGGATAATCCAAGTTGAAATTAACATTAAGAAAAATACCGTGATTACAAAAGAAAGTCTCTTATGTGAATGACACTCGTACCATATTGCAAGAAAAGTTAAAAGAGTTATTGAACCCTTTGGTTTCTTTGTAGCTATAAAACCCACAAAAGAGTTCTACTCTAACCTTGCACTTCCAAATACTTTTGCTTTGTCATTCAGCAACCAGCCCAAGAGAGCTTCAGGAAGCCAAATATGTGACTTTTCTGGAAAAATAAAGGAGATATCAAATTTATTTCATACTTATTTACGCATTGATTTTGGTCATTCTCATGTCATTTTGCCTTCTCTGAGAACATATTAGCTTTGTGTATAATTTAGTATGATGCTGATTGCTCTTCACATTAAAGAAACTGCTCAAATTATTTGCAGTCTCTGAGAGGTCCAAGAGGTGGAATGATCTTCTTCAGGAAGGATCCTGTTCTCGGAGTTGACTTAGAATCTGCCATTAATAATGCTGTGTTCCCAGGATTACAGGTTGGGCCAACCAGTCTTGTGGTTGCACATAAAGATGAATTCATTCGTTTTGCTAGATACCTTGTCTTCGGTTCTTAAATATTGTCAATTTTATGGTTGTCATACAGGGTGGTCCTCATAATCATACAATTGGGGGACTAGCTGTTTGCTTGAAGCATGCACAATCCCCAGAATTTAAGGTCTATCAGAACAAGGTACTTCACTTCCTCAATGATCTAACAATGATGTAAGACATTGTTTTCTTATTTTCTAACAACGTAGCTCTTCACACTTTAGGTCATTTCCAATTGTAGAGCTCTTGCGGGTCGACTTGTGGAGTTAGGTTATAAGCTAGTTTCCGGTGGAAGTGACAATCACCTAGTTCTTGTAGATCTCAGGCCATTGGTAAATCATTGTTCATCATAAATGTGCTTTCAAATAACGTAAGGAAGCATATGTTAATCTATAAGTAAACTCTAACGACAGGGTATCGATGGGGCGCGGGTGGAAAAAATTCTCGACATGGCCTCAATCACTCTCAACAAGAACTCAGTACCTGGTAATGCCTCATGTTTCAACAGGCTGGCCATCATGTCCAATTTTCTTGAACTGTCTCTTAACTGTTTTATGCATAGGTGACAAGAGTGCTCTAGTTCCGGGAGGCATTCGAATCGGGTCGCCTGCCATGACCACCCGAGGATTCACTGAGAATGAGTTTGTAGCGGTTGCAGACTTCATCCATGAGGGTGTTAAGATAACTCTTGATGCCAAACCACTGGCCCCAGGATCAAAGCTCCAAGAGTTCCTCAAGTTCGTGACAACTTCTGATTTCCCTTTGATTGACCGAGTTTCAGATCTTCGCTCGAGAGTCGAAGCTCTTACCACCCAATTCCCAATACCTGGCCTGTAAGTTTTTCACTACACAGAAGGAAATGGTAATAAATTTACAATAACAATTCTGACCAAAAAAGGGGACAGGGTCGGAGGAAATGTCACTTTGTTCTTGATGGGACATGTTTGTGTTGATTGTAGTAATACAAATTAGATAGTTTGTAACATGATATAAGCTGTTGTTGCAGCGATGAGGCTTTGGTGGGAACTGTATCCTTGATAAAATTCTCTTCCTATGTTTTTCATTTTG

mRNA sequence

AAAAAAAAAAAAAAACCCTAAGACCCAAAATGTCTCCTCGTCCCCCTCTTTCACGTTAAGAGCAGCCTCCTCTCCTCCTCTTCTTTCTTTCTGTCTCCCTCTCTTTGCCGTGAAACTCCAGCCGGCAGCCACCCAACCAGCATCATCTTCATCCTTTGCAAGCTTCAATCCACGGGCGACGACGACAAAAACTCCACGGCGGCGTCCCGCGACTTGGAGCAGATAGCGACCCACGCATTCTCTCTGCCTCCGACAGCGCCTGTACGCGACGTCAAGTCCAGCTCCGACGAGTGGCAGCCGAAAACCAATCCGTTGCTGCTCGTTTTGGCTAACCCACGACGTCTGTGACCTCCGAAAACCTTGTAGCAGCAACTTGCGCGACACAAACACCCTGACAACAATCTTTTCCTCTTTTCGGTGGATTCGGAACGATTCAGACACTTTGCAGCAAGGACCCAAGTCTCTTTTGGGATTGTGAAGATTTAAGTGAGAATGCAGGCTACAAGTGGTGCTGCAGTGATGGGCTCTCTGCAAGTTGCTGTTTGTGCGAAGGGATCATGTTTTCCTTCAAAAGGTTCTAGCATCTGTGTGTTTCCACAACACAAGAAAATGAACATCCTGAAACCCTATAGATCCTTTAATGTTGAAGCAAGCATGGTTGCGGGGAAGCCATCATCTTCTGTATCGGTCACTGTTCCAGAAATTGGAGGTGTAAGCCAGTTTGTAGATCATGCCTTGAGTGAAATAGATCCCGAAGTTCGAAGTATTATTAACAAAGAAAAGCAGCGACAATTTAAAAGCCTTGAACTTATTGCCTCGGAGAATTTTACATCTCGGGCAGTTATGGAGGCAGTAGGGTCTTGTCTCACAAACAAATATTCAGAAGGATTACCTGGAAAAAGGTATTATGGTGGCAATGAACATATTGACGAGCTTGAAACACTCTGTCAACAAAGGGCTCTCGAAGCATTTCATTTGGACAATAACAAGTGGGGTGTAAATGTTCAACCGTTATCTGGTTCACCTGCTAACTTCGAGGTTTATACTGCAATTCTTAATCCACATGATCGGATCATGGGTTTAGACTTGCCTCATGGAGGACATTTATCTCATGGGTTTATGACACCTAAAAGGCGGGTGTCAGGCGCATCAATTTATTTTGAATCCATGCCTTATCGTCTTGATGAGACTACAGGCATCGTAGATTATGACATGCTCGAAAAAACTGCTACCTTGTTTCGACCAAAGCTGATTATTGCTGGTGCAAGTGCTTATCCACGAGATTTTGACTATCCTCGCATGAGAAAAATTGCCGATGCTGTTGGTGCTTTTCTCATGATGGATATGGCTCACATAAGTGGGCTTGTTGCTGCTTCTGTGGTTGCTAATCCTTTTGAATACTGTGATATTGTGACCACGACAACACACAAGTCTCTGAGAGGTCCAAGAGGTGGAATGATCTTCTTCAGGAAGGATCCTGTTCTCGGAGTTGACTTAGAATCTGCCATTAATAATGCTGTGTTCCCAGGATTACAGGGTGGTCCTCATAATCATACAATTGGGGGACTAGCTGTTTGCTTGAAGCATGCACAATCCCCAGAATTTAAGGTCTATCAGAACAAGGTCATTTCCAATTGTAGAGCTCTTGCGGGTCGACTTGTGGAGTTAGGTTATAAGCTAGTTTCCGGTGGAAGTGACAATCACCTAGTTCTTGTAGATCTCAGGCCATTGGGTATCGATGGGGCGCGGGTGGAAAAAATTCTCGACATGGCCTCAATCACTCTCAACAAGAACTCAGTACCTGGTGACAAGAGTGCTCTAGTTCCGGGAGGCATTCGAATCGGGTCGCCTGCCATGACCACCCGAGGATTCACTGAGAATGAGTTTGTAGCGGTTGCAGACTTCATCCATGAGGGTGTTAAGATAACTCTTGATGCCAAACCACTGGCCCCAGGATCAAAGCTCCAAGAGTTCCTCAAGTTCGTGACAACTTCTGATTTCCCTTTGATTGACCGAGTTTCAGATCTTCGCTCGAGAGTCGAAGCTCTTACCACCCAATTCCCAATACCTGGCCTGTAAGTTTTTCACTACACAGAAGGAAATGGTAATAAATTTACAATAACAATTCTGACCAAAAAAGGGGACAGGGTCGGAGGAAATGTCACTTTGTTCTTGATGGGACATGTTTGTGTTGATTGTAGTAATACAAATTAGATAGTTTGTAACATGATATAAGCTGTTGTTGCAGCGATGAGGCTTTGGTGGGAACTGTATCCTTGATAAAATTCTCTTCCTATGTTTTTCATTTTG

Coding sequence (CDS)

ATGCAGGCTACAAGTGGTGCTGCAGTGATGGGCTCTCTGCAAGTTGCTGTTTGTGCGAAGGGATCATGTTTTCCTTCAAAAGGTTCTAGCATCTGTGTGTTTCCACAACACAAGAAAATGAACATCCTGAAACCCTATAGATCCTTTAATGTTGAAGCAAGCATGGTTGCGGGGAAGCCATCATCTTCTGTATCGGTCACTGTTCCAGAAATTGGAGGTGTAAGCCAGTTTGTAGATCATGCCTTGAGTGAAATAGATCCCGAAGTTCGAAGTATTATTAACAAAGAAAAGCAGCGACAATTTAAAAGCCTTGAACTTATTGCCTCGGAGAATTTTACATCTCGGGCAGTTATGGAGGCAGTAGGGTCTTGTCTCACAAACAAATATTCAGAAGGATTACCTGGAAAAAGGTATTATGGTGGCAATGAACATATTGACGAGCTTGAAACACTCTGTCAACAAAGGGCTCTCGAAGCATTTCATTTGGACAATAACAAGTGGGGTGTAAATGTTCAACCGTTATCTGGTTCACCTGCTAACTTCGAGGTTTATACTGCAATTCTTAATCCACATGATCGGATCATGGGTTTAGACTTGCCTCATGGAGGACATTTATCTCATGGGTTTATGACACCTAAAAGGCGGGTGTCAGGCGCATCAATTTATTTTGAATCCATGCCTTATCGTCTTGATGAGACTACAGGCATCGTAGATTATGACATGCTCGAAAAAACTGCTACCTTGTTTCGACCAAAGCTGATTATTGCTGGTGCAAGTGCTTATCCACGAGATTTTGACTATCCTCGCATGAGAAAAATTGCCGATGCTGTTGGTGCTTTTCTCATGATGGATATGGCTCACATAAGTGGGCTTGTTGCTGCTTCTGTGGTTGCTAATCCTTTTGAATACTGTGATATTGTGACCACGACAACACACAAGTCTCTGAGAGGTCCAAGAGGTGGAATGATCTTCTTCAGGAAGGATCCTGTTCTCGGAGTTGACTTAGAATCTGCCATTAATAATGCTGTGTTCCCAGGATTACAGGGTGGTCCTCATAATCATACAATTGGGGGACTAGCTGTTTGCTTGAAGCATGCACAATCCCCAGAATTTAAGGTCTATCAGAACAAGGTCATTTCCAATTGTAGAGCTCTTGCGGGTCGACTTGTGGAGTTAGGTTATAAGCTAGTTTCCGGTGGAAGTGACAATCACCTAGTTCTTGTAGATCTCAGGCCATTGGGTATCGATGGGGCGCGGGTGGAAAAAATTCTCGACATGGCCTCAATCACTCTCAACAAGAACTCAGTACCTGGTGACAAGAGTGCTCTAGTTCCGGGAGGCATTCGAATCGGGTCGCCTGCCATGACCACCCGAGGATTCACTGAGAATGAGTTTGTAGCGGTTGCAGACTTCATCCATGAGGGTGTTAAGATAACTCTTGATGCCAAACCACTGGCCCCAGGATCAAAGCTCCAAGAGTTCCTCAAGTTCGTGACAACTTCTGATTTCCCTTTGATTGACCGAGTTTCAGATCTTCGCTCGAGAGTCGAAGCTCTTACCACCCAATTCCCAATACCTGGCCTGTAA

Protein sequence

MQATSGAAVMGSLQVAVCAKGSCFPSKGSSICVFPQHKKMNILKPYRSFNVEASMVAGKPSSSVSVTVPEIGGVSQFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPANFEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYDMLEKTATLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANPFEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITLDAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL
Homology
BLAST of Lsi07G008040 vs. ExPASy Swiss-Prot
Match: Q94JQ3 (Serine hydroxymethyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SHM3 PE=1 SV=2)

HSP 1 Score: 839.3 bits (2167), Expect = 2.3e-242
Identity = 418/529 (79.02%), Postives = 459/529 (86.77%), Query Frame = 0

Query: 1   MQATSGAAVMGSLQVAVCAKGSCFPSKGSSICVFPQHKKMNILKPYRSFNVEASMVAGKP 60
           MQA  G   M SLQ     +GS FP     +  F Q  K NI KP+RS  ++ ++V+   
Sbjct: 1   MQACCGGNSMASLQQPGRVQGSVFPPIMPPVTKFSQQLKFNISKPFRSSFLKRNLVSEMR 60

Query: 61  SSSVSVTVPEIGGVS-QFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVME 120
           +SSVS+   EI      F D+ L E+DPEVR+II KEK RQF+SLELIASENFTSRAVME
Sbjct: 61  ASSVSLPNVEISSKEIPFEDYGLGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVME 120

Query: 121 AVGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPA 180
           AVGSCLTNKYSEGLPGKRYYGGNE+ID+LETLCQ RAL AF LD+ KWGVNVQPLSGSPA
Sbjct: 121 AVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRALAAFRLDSTKWGVNVQPLSGSPA 180

Query: 181 NFEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDY 240
           NF VYTAIL+PHDRIMGLDLPHGGHLSHGFMT KRRVSG SIYFESMPYRLDE+TGIVDY
Sbjct: 181 NFAVYTAILSPHDRIMGLDLPHGGHLSHGFMTAKRRVSGTSIYFESMPYRLDESTGIVDY 240

Query: 241 DMLEKTATLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVAN 300
           DMLEKTATLFRPKLIIAGASAY RDFDYPRMRKIAD+VGAFLMMDMAHISGLVAASVVA+
Sbjct: 241 DMLEKTATLFRPKLIIAGASAYSRDFDYPRMRKIADSVGAFLMMDMAHISGLVAASVVAD 300

Query: 301 PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGL 360
           PFEYCDIVTTTTHKSLRGPRGGMIFFRKDP+ GVDLESA+NNAVFPGLQGGPHNHTIGGL
Sbjct: 301 PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPINGVDLESAVNNAVFPGLQGGPHNHTIGGL 360

Query: 361 AVCLKHAQSPEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGAR 420
           AVCLKHAQSPEFK YQ +V+SNCRALA RLVELG+KLVSGGSDNHLVLVDLRP+G+DGAR
Sbjct: 361 AVCLKHAQSPEFKAYQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGAR 420

Query: 421 VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKIT 480
           VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRG +E +FV VADFI EGV+IT
Sbjct: 421 VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEIT 480

Query: 481 LDAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 529
           ++AK  APGSKLQ+F KFVT+ +FPL +RV  L+ RVE  T++FPIPG+
Sbjct: 481 MEAKKAAPGSKLQDFNKFVTSPEFPLKERVKSLKERVETFTSRFPIPGV 529

BLAST of Lsi07G008040 vs. ExPASy Swiss-Prot
Match: Q9SZJ5 (Serine hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SHM1 PE=1 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 1.5e-164
Identity = 281/454 (61.89%), Postives = 345/454 (75.99%), Query Frame = 0

Query: 82  LSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGG 141
           L E+DPE+  II  EK RQ+K LELI SENFTS +VM+AVGS +TNKYSEG PG RYYGG
Sbjct: 55  LEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGG 114

Query: 142 NEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPANFEVYTAILNPHDRIMGLDLPH 201
           NE+ID  ETLCQ+RALEAF LD  KWGVNVQPLSGSPANF VYTA+L PH+RIM LDLPH
Sbjct: 115 NEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPH 174

Query: 202 GGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYDMLEKTATLFRPKLIIAGASAY 261
           GGHLSHG+ T  +++S  SI+FE+MPYRLDE+TG +DYD +EK+ATLFRPKLI+AGASAY
Sbjct: 175 GGHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAGASAY 234

Query: 262 PRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANPFEYCDIVTTTTHKSLRGPRGG 321
            R +DY R+RK+ +   A ++ DMAHISGLVAA+V+ +PF+Y D+VTTTTHKSLRGPRG 
Sbjct: 235 ARLYDYARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGA 294

Query: 322 MIFFRK--------DPVLGVDLESAINNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFKV 381
           MIFFRK           +  D E  IN AVFPGLQGGPHNHTI GLAV LK A + E+K 
Sbjct: 295 MIFFRKGVKEINKQGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKA 354

Query: 382 YQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEKILDMASITLNK 441
           YQ +V+SN    A  L+E GY+LVSGG+DNHLVLV+L+P GIDG+RVEK+L+   I  NK
Sbjct: 355 YQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNK 414

Query: 442 NSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITLDAKPLAPGSKLQE 501
           N+VPGD SA+VPGGIR+G+PA+T+RGF E +F  VA++  + V I L  K  A G+KL++
Sbjct: 415 NTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTKLKD 474

Query: 502 FLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPG 528
           F+  + +S   +   ++ LR  VE    QFP  G
Sbjct: 475 FVSAMESSS-TIQSEIAKLRHEVEEFAKQFPTIG 507

BLAST of Lsi07G008040 vs. ExPASy Swiss-Prot
Match: P34899 (Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 2.5e-164
Identity = 282/454 (62.11%), Postives = 345/454 (75.99%), Query Frame = 0

Query: 82  LSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGG 141
           L  IDPE+  II  EK RQ+K LELI SENFTS +VM+AVGS +TNKYSEG PG RYYGG
Sbjct: 56  LEVIDPEIADIIELEKARQWKGLELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGG 115

Query: 142 NEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPANFEVYTAILNPHDRIMGLDLPH 201
           NE+ID  ETLCQ+RALEAF LD  KWGVNVQPLSGSP+NF+VYTA+L PHDRIM LDLPH
Sbjct: 116 NEYIDMAETLCQKRALEAFRLDPAKWGVNVQPLSGSPSNFQVYTALLKPHDRIMALDLPH 175

Query: 202 GGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYDMLEKTATLFRPKLIIAGASAY 261
           GGHLSHG+ T  +++S  SI+FE+MPYRLDE+TG +DYD LEK+ATLFRPKLI+AGASAY
Sbjct: 176 GGHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLEKSATLFRPKLIVAGASAY 235

Query: 262 PRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANPFEYCDIVTTTTHKSLRGPRGG 321
            R +DY R+RK+ D   A L+ DMAHISGLVAA V+ +PF+Y D+VTTTTHKSLRGPRG 
Sbjct: 236 ARLYDYARIRKVCDKQKAVLLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGA 295

Query: 322 MIFFRK--------DPVLGVDLESAINNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFKV 381
           MIFFRK           +  D E  IN AVFPGLQGGPHNHTI GLAV LK A +PE++ 
Sbjct: 296 MIFFRKGLKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYRA 355

Query: 382 YQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEKILDMASITLNK 441
           YQ +V+SN    A  L E GY LVSGG++NHLVLV+L+  GIDG+RVEK+L++  I  NK
Sbjct: 356 YQEQVLSNSSKFAKALSEKGYDLVSGGTENHLVLVNLKNKGIDGSRVEKVLELVHIAANK 415

Query: 442 NSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITLDAKPLAPGSKLQE 501
           N+VPGD SA+VPGGIR+G+PA+T+RGF E +FV VA++    V + L  K  + G+KL++
Sbjct: 416 NTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFVKVAEYFDAAVSLALKVKAESKGTKLKD 475

Query: 502 FLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPG 528
           F++ + TS + +   +S L+  VE    QFP  G
Sbjct: 476 FVEALQTSSY-VQSEISKLKHDVEEFAKQFPTIG 508

BLAST of Lsi07G008040 vs. ExPASy Swiss-Prot
Match: P34898 (Serine hydroxymethyltransferase, cytosolic OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=for PE=3 SV=2)

HSP 1 Score: 578.2 bits (1489), Expect = 9.7e-164
Identity = 282/460 (61.30%), Postives = 354/460 (76.96%), Query Frame = 0

Query: 78  VDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKR 137
           ++H+L E DP+V  I+ KE QRQ +S+ LIASEN TSRAV +A+GS ++NKYSEGLPG R
Sbjct: 14  LEHSLVESDPQVAEIMKKEVQRQRESIILIASENVTSRAVFDALGSPMSNKYSEGLPGAR 73

Query: 138 YYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPANFEVYTAILNPHDRIMGL 197
           YYGGN+HIDE+E LCQ RALEAFHLD  +WGVNVQ LSGSPAN +VY AI+  H R+MGL
Sbjct: 74  YYGGNQHIDEIEVLCQNRALEAFHLDPKQWGVNVQCLSGSPANLQVYQAIMPVHGRLMGL 133

Query: 198 DLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYDMLEKTATLFRPKLIIAG 257
           DLPHGGHLSHG+ TP+R++S  S YFE+MPYR++  TG++DYD LEK A LFRPK+++AG
Sbjct: 134 DLPHGGHLSHGYQTPQRKISAVSTYFETMPYRVNIDTGLIDYDTLEKNAQLFRPKVLVAG 193

Query: 258 ASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANPFEYCDIVTTTTHKSLRG 317
            SAY R  DY RMRKIAD+VGA+L++DMAHISGL+A+ V+ +PF Y D+VTTTTHKSLRG
Sbjct: 194 TSAYCRLIDYERMRKIADSVGAYLVVDMAHISGLIASEVIPSPFLYADVVTTTTHKSLRG 253

Query: 318 PRGGMIFFRK-----DPVLG----VDLESAINNAVFPGLQGGPHNHTIGGLAVCLKHAQS 377
           PRG MIFFR+     D   G     DLE  IN +VFPG QGGPHNHTI  LAV LK A S
Sbjct: 254 PRGAMIFFRRGVRSVDAKTGKETLYDLEDKINFSVFPGHQGGPHNHTITALAVALKQAAS 313

Query: 378 PEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEKILDMAS 437
           PEFK YQ KV++N +AL  +L ELGYKLVS G+D+H+VLVDLRP+G+DGARVE +L+  +
Sbjct: 314 PEFKEYQQKVVANAKALEKKLKELGYKLVSDGTDSHMVLVDLRPIGVDGARVEFLLEQIN 373

Query: 438 ITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITLDAKPLAP- 497
           IT NKN+VPGDKSAL PGG+RIG+PAMT+RGF E +F  VA F+ E VK+  + +   P 
Sbjct: 374 ITCNKNAVPGDKSALTPGGLRIGTPAMTSRGFGEADFEKVAVFVDEAVKLCKEIQASLPK 433

Query: 498 -GSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIP 527
             +K ++F   + TSD P   R+++L+  + A +  FP+P
Sbjct: 434 EANKQKDFKAKIATSDIP---RINELKQEIAAWSNTFPLP 470

BLAST of Lsi07G008040 vs. ExPASy Swiss-Prot
Match: Q94C74 (Serine hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SHM2 PE=1 SV=2)

HSP 1 Score: 577.8 bits (1488), Expect = 1.3e-163
Identity = 282/455 (61.98%), Postives = 345/455 (75.82%), Query Frame = 0

Query: 81  ALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYG 140
           +L EIDPEV  II  EK RQ+K  ELI SENFTS +VM+AVGS +TNKYSEG PG RYYG
Sbjct: 54  SLDEIDPEVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYG 113

Query: 141 GNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPANFEVYTAILNPHDRIMGLDLP 200
           GNE+ID  ETLCQ+RALEAF LD +KWGVNVQ LSGSPANF+VYTA+L PH+RIM LDLP
Sbjct: 114 GNEYIDMAETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLP 173

Query: 201 HGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYDMLEKTATLFRPKLIIAGASA 260
           HGGHLSHG+ T  +++S  SI+FE+MPYRLDE TG +DYD LEK+A LFRPKLI+AGASA
Sbjct: 174 HGGHLSHGYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASA 233

Query: 261 YPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANPFEYCDIVTTTTHKSLRGPRG 320
           Y R +DY R+RK+ +   A ++ DMAHISGLVAA V+ +PFEY D+VTTTTHKSLRGPRG
Sbjct: 234 YARLYDYARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRG 293

Query: 321 GMIFFRK--------DPVLGVDLESAINNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFK 380
            MIFFRK           +  D E  IN AVFPGLQGGPHNHTI GLAV LK A++PE+K
Sbjct: 294 AMIFFRKGLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYK 353

Query: 381 VYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEKILDMASITLN 440
            YQ++V+ NC   A  L+  GY LVSGG+DNHLVLV+L+  GIDG+RVEK+L++  I  N
Sbjct: 354 AYQDQVLRNCSKFAETLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAAN 413

Query: 441 KNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITLDAKPLAPGSKLQ 500
           KN+VPGD SA+VPGGIR+G+PA+T+RGF E +F  VA++    VKI L  K  + G+KL+
Sbjct: 414 KNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIKAESQGTKLK 473

Query: 501 EFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPG 528
           +F+  + +++  L   +S LR  VE    QFP  G
Sbjct: 474 DFVATMQSNE-KLQSEMSKLREMVEEYAKQFPTIG 507

BLAST of Lsi07G008040 vs. ExPASy TrEMBL
Match: A0A0A0KYS6 (Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_4G062380 PE=3 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 8.5e-296
Identity = 508/528 (96.21%), Postives = 514/528 (97.35%), Query Frame = 0

Query: 1   MQATSGAAVMGSLQVAVCAKGSCFPSKGSSICVFPQHKKMNILKPYRSFNVEASMVAGKP 60
           MQATSG AVMGSLQVAVC KGSCFPSK SSICVFPQ KKMNILKP +SF VEASMVAGKP
Sbjct: 1   MQATSGVAVMGSLQVAVCGKGSCFPSKSSSICVFPQQKKMNILKPCKSFKVEASMVAGKP 60

Query: 61  SSSVSVTVPEIGGVSQFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
           SSSVSVTVPEIGGVS FVDHALSE DPEVRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSVSVTVPEIGGVSNFVDHALSETDPEVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRAL AFHLDNNKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDNNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYD 240
           FEVYTA+LNPHDRIMGLDLPHGGHLSHGFMTPKRRVSG SIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAVLNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTATLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANP 300
           MLEKTA LFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVA+P
Sbjct: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEFKVYQNKVI+NCRALA RLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKVYQNKVIANCRALANRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTE EFVAVADFIHEGVKITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFVAVADFIHEGVKITL 480

Query: 481 DAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 529
           DAKPLAPGSKLQEFLKFVTTSDFPL DR+SDLRSRVEALTTQFPIPGL
Sbjct: 481 DAKPLAPGSKLQEFLKFVTTSDFPLTDRISDLRSRVEALTTQFPIPGL 528

BLAST of Lsi07G008040 vs. ExPASy TrEMBL
Match: A0A5D3D173 (Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G002230 PE=3 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 7.9e-294
Identity = 504/528 (95.45%), Postives = 512/528 (96.97%), Query Frame = 0

Query: 1   MQATSGAAVMGSLQVAVCAKGSCFPSKGSSICVFPQHKKMNILKPYRSFNVEASMVAGKP 60
           MQATSGAAVMGSL+VAVC KGSCFPSK SSICVFPQHKKMNILKP +S  V+ASMV GKP
Sbjct: 1   MQATSGAAVMGSLEVAVCGKGSCFPSKSSSICVFPQHKKMNILKPSKSSKVKASMVVGKP 60

Query: 61  SSSVSVTVPEIGGVSQFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
           SSS SVTVPEIGGVS FVDHALSE DPEVRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSASVTVPEIGGVSHFVDHALSETDPEVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRAL AFHLDNNKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDNNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYD 240
           FEVYTA+LNPHDRIMGLDLPHGGHLSHGFMTPKRRVSG SIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAVLNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTATLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANP 300
           MLE+TA LFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVA+P
Sbjct: 241 MLERTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEFKVYQNKVI+NCRALA RLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKVYQNKVIANCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTE EF AVADFIHEGVKITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFAAVADFIHEGVKITL 480

Query: 481 DAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 529
           DAKPLAPGSKLQEFLKFVTTSDFPL DRVSDLRSRVEALTTQFPIPGL
Sbjct: 481 DAKPLAPGSKLQEFLKFVTTSDFPLTDRVSDLRSRVEALTTQFPIPGL 528

BLAST of Lsi07G008040 vs. ExPASy TrEMBL
Match: A0A1S3BSI5 (Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103493017 PE=3 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 7.9e-294
Identity = 504/528 (95.45%), Postives = 512/528 (96.97%), Query Frame = 0

Query: 1   MQATSGAAVMGSLQVAVCAKGSCFPSKGSSICVFPQHKKMNILKPYRSFNVEASMVAGKP 60
           MQATSGAAVMGSL+VAVC KGSCFPSK SSICVFPQHKKMNILKP +S  V+ASMV GKP
Sbjct: 1   MQATSGAAVMGSLEVAVCGKGSCFPSKSSSICVFPQHKKMNILKPSKSSKVKASMVVGKP 60

Query: 61  SSSVSVTVPEIGGVSQFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
           SSS SVTVPEIGGVS FVDHALSE DPEVRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSASVTVPEIGGVSHFVDHALSETDPEVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRAL AFHLDNNKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDNNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYD 240
           FEVYTA+LNPHDRIMGLDLPHGGHLSHGFMTPKRRVSG SIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAVLNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTATLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANP 300
           MLE+TA LFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVA+P
Sbjct: 241 MLERTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEFKVYQNKVI+NCRALA RLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKVYQNKVIANCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTE EF AVADFIHEGVKITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFAAVADFIHEGVKITL 480

Query: 481 DAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 529
           DAKPLAPGSKLQEFLKFVTTSDFPL DRVSDLRSRVEALTTQFPIPGL
Sbjct: 481 DAKPLAPGSKLQEFLKFVTTSDFPLTDRVSDLRSRVEALTTQFPIPGL 528

BLAST of Lsi07G008040 vs. ExPASy TrEMBL
Match: A0A5A7V8Z1 (Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold468G00080 PE=3 SV=1)

HSP 1 Score: 1015.0 bits (2623), Expect = 1.1e-292
Identity = 502/526 (95.44%), Postives = 510/526 (96.96%), Query Frame = 0

Query: 3   ATSGAAVMGSLQVAVCAKGSCFPSKGSSICVFPQHKKMNILKPYRSFNVEASMVAGKPSS 62
           ATSGAAVMGSL+VAVC KGSCFPSK SSICVFPQHKKMNILKP +S  V+ASMV GKPSS
Sbjct: 36  ATSGAAVMGSLEVAVCGKGSCFPSKSSSICVFPQHKKMNILKPSKSSKVKASMVVGKPSS 95

Query: 63  SVSVTVPEIGGVSQFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVG 122
           S SVTVPEIGGVS FVDHALSE DPEVRSII+KEKQRQFKSLELIASENFTSRAVMEAVG
Sbjct: 96  SASVTVPEIGGVSHFVDHALSETDPEVRSIIDKEKQRQFKSLELIASENFTSRAVMEAVG 155

Query: 123 SCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPANFE 182
           SCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRAL AFHLDNNKWGVNVQPLSGSPANFE
Sbjct: 156 SCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDNNKWGVNVQPLSGSPANFE 215

Query: 183 VYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYDML 242
           VYTA+LNPHDRIMGLDLPHGGHLSHGFMTPKRRVSG SIYFESMPYRLDETTGIVDYDML
Sbjct: 216 VYTAVLNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYDML 275

Query: 243 EKTATLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANPFE 302
           E+TA LFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVA+PFE
Sbjct: 276 ERTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADPFE 335

Query: 303 YCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLAVC 362
           YCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLAVC
Sbjct: 336 YCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLAVC 395

Query: 363 LKHAQSPEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEK 422
           LKHAQSPEFKVYQNKVI+NCRALA RLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEK
Sbjct: 396 LKHAQSPEFKVYQNKVIANCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEK 455

Query: 423 ILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITLDA 482
           ILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTE EF AVADFIHEGVKITLDA
Sbjct: 456 ILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFAAVADFIHEGVKITLDA 515

Query: 483 KPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 529
           KPLAPGSKLQEFLKFVTTSDFPL DRVSDLRSRVEALTTQFPIPGL
Sbjct: 516 KPLAPGSKLQEFLKFVTTSDFPLTDRVSDLRSRVEALTTQFPIPGL 561

BLAST of Lsi07G008040 vs. ExPASy TrEMBL
Match: A0A6J1FS47 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447665 PE=3 SV=1)

HSP 1 Score: 996.9 bits (2576), Expect = 3.2e-287
Identity = 494/528 (93.56%), Postives = 507/528 (96.02%), Query Frame = 0

Query: 1   MQATSGAAVMGSLQVAVCAKGSCFPSKGSSICVFPQHKKMNILKPYRSFNVEASMVAGKP 60
           MQA SGAAVMGSLQV VCAKGS FPSKGSSICVFPQHKK  +LKP +S +VEA+MVAGK 
Sbjct: 1   MQAASGAAVMGSLQVVVCAKGSSFPSKGSSICVFPQHKKWTVLKPCKSSSVEANMVAGKA 60

Query: 61  SSSVSVTVPEIGGVSQFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
           SSSVSV+ P++GG S FVDH LSE DP+VRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSVSVSAPQLGGGSGFVDHGLSEADPDVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRAL AF LDNNKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFRLDNNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYD 240
           FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSG SIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTATLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANP 300
           MLEKTA LFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVA+P
Sbjct: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEFK YQNKV+SNCRALA RLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKDYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTE EF+AVADFIHEGVKITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVKITL 480

Query: 481 DAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 529
           DAKPLAPGSKLQEFLKFV TSDFPLIDRVS+LRSRVEALTTQFPIPGL
Sbjct: 481 DAKPLAPGSKLQEFLKFVATSDFPLIDRVSNLRSRVEALTTQFPIPGL 528

BLAST of Lsi07G008040 vs. NCBI nr
Match: XP_011653260.1 (serine hydroxymethyltransferase 3, chloroplastic [Cucumis sativus] >XP_031740593.1 serine hydroxymethyltransferase 3, chloroplastic [Cucumis sativus] >KGN53502.1 hypothetical protein Csa_014386 [Cucumis sativus])

HSP 1 Score: 1025.4 bits (2650), Expect = 1.7e-295
Identity = 508/528 (96.21%), Postives = 514/528 (97.35%), Query Frame = 0

Query: 1   MQATSGAAVMGSLQVAVCAKGSCFPSKGSSICVFPQHKKMNILKPYRSFNVEASMVAGKP 60
           MQATSG AVMGSLQVAVC KGSCFPSK SSICVFPQ KKMNILKP +SF VEASMVAGKP
Sbjct: 1   MQATSGVAVMGSLQVAVCGKGSCFPSKSSSICVFPQQKKMNILKPCKSFKVEASMVAGKP 60

Query: 61  SSSVSVTVPEIGGVSQFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
           SSSVSVTVPEIGGVS FVDHALSE DPEVRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSVSVTVPEIGGVSNFVDHALSETDPEVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRAL AFHLDNNKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDNNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYD 240
           FEVYTA+LNPHDRIMGLDLPHGGHLSHGFMTPKRRVSG SIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAVLNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTATLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANP 300
           MLEKTA LFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVA+P
Sbjct: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEFKVYQNKVI+NCRALA RLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKVYQNKVIANCRALANRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTE EFVAVADFIHEGVKITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFVAVADFIHEGVKITL 480

Query: 481 DAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 529
           DAKPLAPGSKLQEFLKFVTTSDFPL DR+SDLRSRVEALTTQFPIPGL
Sbjct: 481 DAKPLAPGSKLQEFLKFVTTSDFPLTDRISDLRSRVEALTTQFPIPGL 528

BLAST of Lsi07G008040 vs. NCBI nr
Match: XP_038877982.1 (serine hydroxymethyltransferase 3, chloroplastic [Benincasa hispida] >XP_038877983.1 serine hydroxymethyltransferase 3, chloroplastic [Benincasa hispida] >XP_038877984.1 serine hydroxymethyltransferase 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 1024.2 bits (2647), Expect = 3.9e-295
Identity = 510/528 (96.59%), Postives = 518/528 (98.11%), Query Frame = 0

Query: 1   MQATSGAAVMGSLQVAVCAKGSCFPSKGSSICVFPQHKKMNILKPYRSFNVEASMVAGKP 60
           MQATSGAAVMGSLQVAVCAKGSCFPSK SS CVFPQHKKM ILKP +S NVEASMVAGKP
Sbjct: 1   MQATSGAAVMGSLQVAVCAKGSCFPSKSSS-CVFPQHKKMTILKPCKSSNVEASMVAGKP 60

Query: 61  SSSVSVTVPEIGGVSQFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
           SSSVSVTVPEIGGVSQFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSVSVTVPEIGGVSQFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRAL AFHLDNNKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDNNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYD 240
           FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSG SIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTATLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANP 300
           MLEKTA LFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVA+P
Sbjct: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FEYCDIVTTTTHKSLRGPRGGMIFF+K+PVLGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEYCDIVTTTTHKSLRGPRGGMIFFKKEPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEFKVYQNKVISNCRALA RLV+LGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKVYQNKVISNCRALASRLVKLGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTE EFVAVADFIHEGVKITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFVAVADFIHEGVKITL 480

Query: 481 DAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 529
           DAKPLAPGSKLQ+FLKFVTTSDFPLIDRVSDLR+RVEALTTQFP+PGL
Sbjct: 481 DAKPLAPGSKLQDFLKFVTTSDFPLIDRVSDLRARVEALTTQFPMPGL 527

BLAST of Lsi07G008040 vs. NCBI nr
Match: XP_008451853.1 (PREDICTED: serine hydroxymethyltransferase 3, chloroplastic [Cucumis melo] >TYK16426.1 serine hydroxymethyltransferase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.6e-293
Identity = 504/528 (95.45%), Postives = 512/528 (96.97%), Query Frame = 0

Query: 1   MQATSGAAVMGSLQVAVCAKGSCFPSKGSSICVFPQHKKMNILKPYRSFNVEASMVAGKP 60
           MQATSGAAVMGSL+VAVC KGSCFPSK SSICVFPQHKKMNILKP +S  V+ASMV GKP
Sbjct: 1   MQATSGAAVMGSLEVAVCGKGSCFPSKSSSICVFPQHKKMNILKPSKSSKVKASMVVGKP 60

Query: 61  SSSVSVTVPEIGGVSQFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
           SSS SVTVPEIGGVS FVDHALSE DPEVRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSASVTVPEIGGVSHFVDHALSETDPEVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRAL AFHLDNNKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDNNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYD 240
           FEVYTA+LNPHDRIMGLDLPHGGHLSHGFMTPKRRVSG SIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAVLNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTATLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANP 300
           MLE+TA LFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVA+P
Sbjct: 241 MLERTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEFKVYQNKVI+NCRALA RLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKVYQNKVIANCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTE EF AVADFIHEGVKITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFAAVADFIHEGVKITL 480

Query: 481 DAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 529
           DAKPLAPGSKLQEFLKFVTTSDFPL DRVSDLRSRVEALTTQFPIPGL
Sbjct: 481 DAKPLAPGSKLQEFLKFVTTSDFPLTDRVSDLRSRVEALTTQFPIPGL 528

BLAST of Lsi07G008040 vs. NCBI nr
Match: KAA0062916.1 (serine hydroxymethyltransferase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1015.0 bits (2623), Expect = 2.4e-292
Identity = 502/526 (95.44%), Postives = 510/526 (96.96%), Query Frame = 0

Query: 3   ATSGAAVMGSLQVAVCAKGSCFPSKGSSICVFPQHKKMNILKPYRSFNVEASMVAGKPSS 62
           ATSGAAVMGSL+VAVC KGSCFPSK SSICVFPQHKKMNILKP +S  V+ASMV GKPSS
Sbjct: 36  ATSGAAVMGSLEVAVCGKGSCFPSKSSSICVFPQHKKMNILKPSKSSKVKASMVVGKPSS 95

Query: 63  SVSVTVPEIGGVSQFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVG 122
           S SVTVPEIGGVS FVDHALSE DPEVRSII+KEKQRQFKSLELIASENFTSRAVMEAVG
Sbjct: 96  SASVTVPEIGGVSHFVDHALSETDPEVRSIIDKEKQRQFKSLELIASENFTSRAVMEAVG 155

Query: 123 SCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPANFE 182
           SCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRAL AFHLDNNKWGVNVQPLSGSPANFE
Sbjct: 156 SCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDNNKWGVNVQPLSGSPANFE 215

Query: 183 VYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYDML 242
           VYTA+LNPHDRIMGLDLPHGGHLSHGFMTPKRRVSG SIYFESMPYRLDETTGIVDYDML
Sbjct: 216 VYTAVLNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYDML 275

Query: 243 EKTATLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANPFE 302
           E+TA LFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVA+PFE
Sbjct: 276 ERTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADPFE 335

Query: 303 YCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLAVC 362
           YCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLAVC
Sbjct: 336 YCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLAVC 395

Query: 363 LKHAQSPEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEK 422
           LKHAQSPEFKVYQNKVI+NCRALA RLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEK
Sbjct: 396 LKHAQSPEFKVYQNKVIANCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEK 455

Query: 423 ILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITLDA 482
           ILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTE EF AVADFIHEGVKITLDA
Sbjct: 456 ILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFAAVADFIHEGVKITLDA 515

Query: 483 KPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 529
           KPLAPGSKLQEFLKFVTTSDFPL DRVSDLRSRVEALTTQFPIPGL
Sbjct: 516 KPLAPGSKLQEFLKFVTTSDFPLTDRVSDLRSRVEALTTQFPIPGL 561

BLAST of Lsi07G008040 vs. NCBI nr
Match: XP_022942709.1 (serine hydroxymethyltransferase 3, chloroplastic-like [Cucurbita moschata] >XP_022942710.1 serine hydroxymethyltransferase 3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 996.9 bits (2576), Expect = 6.6e-287
Identity = 494/528 (93.56%), Postives = 507/528 (96.02%), Query Frame = 0

Query: 1   MQATSGAAVMGSLQVAVCAKGSCFPSKGSSICVFPQHKKMNILKPYRSFNVEASMVAGKP 60
           MQA SGAAVMGSLQV VCAKGS FPSKGSSICVFPQHKK  +LKP +S +VEA+MVAGK 
Sbjct: 1   MQAASGAAVMGSLQVVVCAKGSSFPSKGSSICVFPQHKKWTVLKPCKSSSVEANMVAGKA 60

Query: 61  SSSVSVTVPEIGGVSQFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
           SSSVSV+ P++GG S FVDH LSE DP+VRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSVSVSAPQLGGGSGFVDHGLSEADPDVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRAL AF LDNNKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFRLDNNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYD 240
           FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSG SIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTATLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANP 300
           MLEKTA LFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVA+P
Sbjct: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEFK YQNKV+SNCRALA RLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKDYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTE EF+AVADFIHEGVKITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVKITL 480

Query: 481 DAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 529
           DAKPLAPGSKLQEFLKFV TSDFPLIDRVS+LRSRVEALTTQFPIPGL
Sbjct: 481 DAKPLAPGSKLQEFLKFVATSDFPLIDRVSNLRSRVEALTTQFPIPGL 528

BLAST of Lsi07G008040 vs. TAIR 10
Match: AT4G32520.1 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 839.3 bits (2167), Expect = 1.7e-243
Identity = 418/529 (79.02%), Postives = 459/529 (86.77%), Query Frame = 0

Query: 1   MQATSGAAVMGSLQVAVCAKGSCFPSKGSSICVFPQHKKMNILKPYRSFNVEASMVAGKP 60
           MQA  G   M SLQ     +GS FP     +  F Q  K NI KP+RS  ++ ++V+   
Sbjct: 1   MQACCGGNSMASLQQPGRVQGSVFPPIMPPVTKFSQQLKFNISKPFRSSFLKRNLVSEMR 60

Query: 61  SSSVSVTVPEIGGVS-QFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVME 120
           +SSVS+   EI      F D+ L E+DPEVR+II KEK RQF+SLELIASENFTSRAVME
Sbjct: 61  ASSVSLPNVEISSKEIPFEDYGLGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVME 120

Query: 121 AVGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPA 180
           AVGSCLTNKYSEGLPGKRYYGGNE+ID+LETLCQ RAL AF LD+ KWGVNVQPLSGSPA
Sbjct: 121 AVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRALAAFRLDSTKWGVNVQPLSGSPA 180

Query: 181 NFEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDY 240
           NF VYTAIL+PHDRIMGLDLPHGGHLSHGFMT KRRVSG SIYFESMPYRLDE+TGIVDY
Sbjct: 181 NFAVYTAILSPHDRIMGLDLPHGGHLSHGFMTAKRRVSGTSIYFESMPYRLDESTGIVDY 240

Query: 241 DMLEKTATLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVAN 300
           DMLEKTATLFRPKLIIAGASAY RDFDYPRMRKIAD+VGAFLMMDMAHISGLVAASVVA+
Sbjct: 241 DMLEKTATLFRPKLIIAGASAYSRDFDYPRMRKIADSVGAFLMMDMAHISGLVAASVVAD 300

Query: 301 PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGL 360
           PFEYCDIVTTTTHKSLRGPRGGMIFFRKDP+ GVDLESA+NNAVFPGLQGGPHNHTIGGL
Sbjct: 301 PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPINGVDLESAVNNAVFPGLQGGPHNHTIGGL 360

Query: 361 AVCLKHAQSPEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGAR 420
           AVCLKHAQSPEFK YQ +V+SNCRALA RLVELG+KLVSGGSDNHLVLVDLRP+G+DGAR
Sbjct: 361 AVCLKHAQSPEFKAYQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGAR 420

Query: 421 VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKIT 480
           VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRG +E +FV VADFI EGV+IT
Sbjct: 421 VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEIT 480

Query: 481 LDAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 529
           ++AK  APGSKLQ+F KFVT+ +FPL +RV  L+ RVE  T++FPIPG+
Sbjct: 481 MEAKKAAPGSKLQDFNKFVTSPEFPLKERVKSLKERVETFTSRFPIPGV 529

BLAST of Lsi07G008040 vs. TAIR 10
Match: AT4G32520.2 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 839.3 bits (2167), Expect = 1.7e-243
Identity = 418/529 (79.02%), Postives = 459/529 (86.77%), Query Frame = 0

Query: 1   MQATSGAAVMGSLQVAVCAKGSCFPSKGSSICVFPQHKKMNILKPYRSFNVEASMVAGKP 60
           MQA  G   M SLQ     +GS FP     +  F Q  K NI KP+RS  ++ ++V+   
Sbjct: 1   MQACCGGNSMASLQQPGRVQGSVFPPIMPPVTKFSQQLKFNISKPFRSSFLKRNLVSEMR 60

Query: 61  SSSVSVTVPEIGGVS-QFVDHALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVME 120
           +SSVS+   EI      F D+ L E+DPEVR+II KEK RQF+SLELIASENFTSRAVME
Sbjct: 61  ASSVSLPNVEISSKEIPFEDYGLGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVME 120

Query: 121 AVGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPA 180
           AVGSCLTNKYSEGLPGKRYYGGNE+ID+LETLCQ RAL AF LD+ KWGVNVQPLSGSPA
Sbjct: 121 AVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRALAAFRLDSTKWGVNVQPLSGSPA 180

Query: 181 NFEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDY 240
           NF VYTAIL+PHDRIMGLDLPHGGHLSHGFMT KRRVSG SIYFESMPYRLDE+TGIVDY
Sbjct: 181 NFAVYTAILSPHDRIMGLDLPHGGHLSHGFMTAKRRVSGTSIYFESMPYRLDESTGIVDY 240

Query: 241 DMLEKTATLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVAN 300
           DMLEKTATLFRPKLIIAGASAY RDFDYPRMRKIAD+VGAFLMMDMAHISGLVAASVVA+
Sbjct: 241 DMLEKTATLFRPKLIIAGASAYSRDFDYPRMRKIADSVGAFLMMDMAHISGLVAASVVAD 300

Query: 301 PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGL 360
           PFEYCDIVTTTTHKSLRGPRGGMIFFRKDP+ GVDLESA+NNAVFPGLQGGPHNHTIGGL
Sbjct: 301 PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPINGVDLESAVNNAVFPGLQGGPHNHTIGGL 360

Query: 361 AVCLKHAQSPEFKVYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGAR 420
           AVCLKHAQSPEFK YQ +V+SNCRALA RLVELG+KLVSGGSDNHLVLVDLRP+G+DGAR
Sbjct: 361 AVCLKHAQSPEFKAYQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGAR 420

Query: 421 VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKIT 480
           VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRG +E +FV VADFI EGV+IT
Sbjct: 421 VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEIT 480

Query: 481 LDAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 529
           ++AK  APGSKLQ+F KFVT+ +FPL +RV  L+ RVE  T++FPIPG+
Sbjct: 481 MEAKKAAPGSKLQDFNKFVTSPEFPLKERVKSLKERVETFTSRFPIPGV 529

BLAST of Lsi07G008040 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 580.9 bits (1496), Expect = 1.1e-165
Identity = 281/454 (61.89%), Postives = 345/454 (75.99%), Query Frame = 0

Query: 82  LSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGG 141
           L E+DPE+  II  EK RQ+K LELI SENFTS +VM+AVGS +TNKYSEG PG RYYGG
Sbjct: 55  LEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGG 114

Query: 142 NEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPANFEVYTAILNPHDRIMGLDLPH 201
           NE+ID  ETLCQ+RALEAF LD  KWGVNVQPLSGSPANF VYTA+L PH+RIM LDLPH
Sbjct: 115 NEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPH 174

Query: 202 GGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYDMLEKTATLFRPKLIIAGASAY 261
           GGHLSHG+ T  +++S  SI+FE+MPYRLDE+TG +DYD +EK+ATLFRPKLI+AGASAY
Sbjct: 175 GGHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAGASAY 234

Query: 262 PRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANPFEYCDIVTTTTHKSLRGPRGG 321
            R +DY R+RK+ +   A ++ DMAHISGLVAA+V+ +PF+Y D+VTTTTHKSLRGPRG 
Sbjct: 235 ARLYDYARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGA 294

Query: 322 MIFFRK--------DPVLGVDLESAINNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFKV 381
           MIFFRK           +  D E  IN AVFPGLQGGPHNHTI GLAV LK A + E+K 
Sbjct: 295 MIFFRKGVKEINKQGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKA 354

Query: 382 YQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEKILDMASITLNK 441
           YQ +V+SN    A  L+E GY+LVSGG+DNHLVLV+L+P GIDG+RVEK+L+   I  NK
Sbjct: 355 YQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNK 414

Query: 442 NSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITLDAKPLAPGSKLQE 501
           N+VPGD SA+VPGGIR+G+PA+T+RGF E +F  VA++  + V I L  K  A G+KL++
Sbjct: 415 NTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTKLKD 474

Query: 502 FLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPG 528
           F+  + +S   +   ++ LR  VE    QFP  G
Sbjct: 475 FVSAMESSS-TIQSEIAKLRHEVEEFAKQFPTIG 507

BLAST of Lsi07G008040 vs. TAIR 10
Match: AT5G26780.1 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 577.8 bits (1488), Expect = 9.0e-165
Identity = 282/455 (61.98%), Postives = 345/455 (75.82%), Query Frame = 0

Query: 81  ALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYG 140
           +L EIDPEV  II  EK RQ+K  ELI SENFTS +VM+AVGS +TNKYSEG PG RYYG
Sbjct: 54  SLDEIDPEVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYG 113

Query: 141 GNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPANFEVYTAILNPHDRIMGLDLP 200
           GNE+ID  ETLCQ+RALEAF LD +KWGVNVQ LSGSPANF+VYTA+L PH+RIM LDLP
Sbjct: 114 GNEYIDMAETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLP 173

Query: 201 HGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYDMLEKTATLFRPKLIIAGASA 260
           HGGHLSHG+ T  +++S  SI+FE+MPYRLDE TG +DYD LEK+A LFRPKLI+AGASA
Sbjct: 174 HGGHLSHGYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASA 233

Query: 261 YPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANPFEYCDIVTTTTHKSLRGPRG 320
           Y R +DY R+RK+ +   A ++ DMAHISGLVAA V+ +PFEY D+VTTTTHKSLRGPRG
Sbjct: 234 YARLYDYARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRG 293

Query: 321 GMIFFRK--------DPVLGVDLESAINNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFK 380
            MIFFRK           +  D E  IN AVFPGLQGGPHNHTI GLAV LK A++PE+K
Sbjct: 294 AMIFFRKGLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYK 353

Query: 381 VYQNKVISNCRALAGRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEKILDMASITLN 440
            YQ++V+ NC   A  L+  GY LVSGG+DNHLVLV+L+  GIDG+RVEK+L++  I  N
Sbjct: 354 AYQDQVLRNCSKFAETLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAAN 413

Query: 441 KNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGVKITLDAKPLAPGSKLQ 500
           KN+VPGD SA+VPGGIR+G+PA+T+RGF E +F  VA++    VKI L  K  + G+KL+
Sbjct: 414 KNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIKAESQGTKLK 473

Query: 501 EFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPG 528
           +F+  + +++  L   +S LR  VE    QFP  G
Sbjct: 474 DFVATMQSNE-KLQSEMSKLREMVEEYAKQFPTIG 507

BLAST of Lsi07G008040 vs. TAIR 10
Match: AT5G26780.3 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 567.4 bits (1461), Expect = 1.2e-161
Identity = 282/471 (59.87%), Postives = 345/471 (73.25%), Query Frame = 0

Query: 81  ALSEIDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYG 140
           +L EIDPEV  II  EK RQ+K  ELI SENFTS +VM+AVGS +TNKYSEG PG RYYG
Sbjct: 54  SLDEIDPEVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYG 113

Query: 141 GNEHIDELETLCQQRALEAFHLDNNKWGVNVQPLSGSPANFEVYTAILNPHDRIMGLDLP 200
           GNE+ID  ETLCQ+RALEAF LD +KWGVNVQ LSGSPANF+VYTA+L PH+RIM LDLP
Sbjct: 114 GNEYIDMAETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLP 173

Query: 201 HGGHLSHGFMTPKRRVSGASIYFESMPYRLDETTGIVDYDMLEKTATLFRPKLIIAGASA 260
           HGGHLSHG+ T  +++S  SI+FE+MPYRLDE TG +DYD LEK+A LFRPKLI+AGASA
Sbjct: 174 HGGHLSHGYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASA 233

Query: 261 YPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVANPFEYCDIVTTTTHKSLRGPRG 320
           Y R +DY R+RK+ +   A ++ DMAHISGLVAA V+ +PFEY D+VTTTTHKSLRGPRG
Sbjct: 234 YARLYDYARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRG 293

Query: 321 GMIFFRK--------DPVLGVDLESAINNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFK 380
            MIFFRK           +  D E  IN AVFPGLQGGPHNHTI GLAV LK A++PE+K
Sbjct: 294 AMIFFRKGLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYK 353

Query: 381 VYQNKVISNCRALA----------------GRLVELGYKLVSGGSDNHLVLVDLRPLGID 440
            YQ++V+ NC   A                  L+  GY LVSGG+DNHLVLV+L+  GID
Sbjct: 354 AYQDQVLRNCSKFAELDIRPTVIISYGLSMQTLLAKGYDLVSGGTDNHLVLVNLKNKGID 413

Query: 441 GARVEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTENEFVAVADFIHEGV 500
           G+RVEK+L++  I  NKN+VPGD SA+VPGGIR+G+PA+T+RGF E +F  VA++    V
Sbjct: 414 GSRVEKVLELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAV 473

Query: 501 KITLDAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPG 528
           KI L  K  + G+KL++F+  + +++  L   +S LR  VE    QFP  G
Sbjct: 474 KIALKIKAESQGTKLKDFVATMQSNE-KLQSEMSKLREMVEEYAKQFPTIG 523

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94JQ32.3e-24279.02Serine hydroxymethyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q9SZJ51.5e-16461.89Serine hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702... [more]
P348992.5e-16462.11Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum OX=3888 PE=1 SV=... [more]
P348989.7e-16461.30Serine hydroxymethyltransferase, cytosolic OS=Neurospora crassa (strain ATCC 246... [more]
Q94C741.3e-16361.98Serine hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A0A0KYS68.5e-29696.21Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_4G062380 PE=3 ... [more]
A0A5D3D1737.9e-29495.45Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3BSI57.9e-29495.45Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103493017 PE=3 SV=... [more]
A0A5A7V8Z11.1e-29295.44Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A6J1FS473.2e-28793.56Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447665 PE... [more]
Match NameE-valueIdentityDescription
XP_011653260.11.7e-29596.21serine hydroxymethyltransferase 3, chloroplastic [Cucumis sativus] >XP_031740593... [more]
XP_038877982.13.9e-29596.59serine hydroxymethyltransferase 3, chloroplastic [Benincasa hispida] >XP_0388779... [more]
XP_008451853.11.6e-29395.45PREDICTED: serine hydroxymethyltransferase 3, chloroplastic [Cucumis melo] >TYK1... [more]
KAA0062916.12.4e-29295.44serine hydroxymethyltransferase 3 [Cucumis melo var. makuwa][more]
XP_022942709.16.6e-28793.56serine hydroxymethyltransferase 3, chloroplastic-like [Cucurbita moschata] >XP_0... [more]
Match NameE-valueIdentityDescription
AT4G32520.11.7e-24379.02serine hydroxymethyltransferase 3 [more]
AT4G32520.21.7e-24379.02serine hydroxymethyltransferase 3 [more]
AT4G37930.11.1e-16561.89serine transhydroxymethyltransferase 1 [more]
AT5G26780.19.0e-16561.98serine hydroxymethyltransferase 2 [more]
AT5G26780.31.2e-16159.87serine hydroxymethyltransferase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 111..376
e-value: 9.0E-213
score: 708.5
IPR001085Serine hydroxymethyltransferasePIRSFPIRSF000412SHMTcoord: 63..528
e-value: 2.2E-174
score: 577.9
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 41..528
IPR001085Serine hydroxymethyltransferaseHAMAPMF_00051SHMTcoord: 81..527
score: 44.283676
IPR001085Serine hydroxymethyltransferaseCDDcd00378SHMTcoord: 84..486
e-value: 0.0
score: 648.108
IPR039429Serine hydroxymethyltransferase-like domainPFAMPF00464SHMTcoord: 82..472
e-value: 4.0E-195
score: 648.0
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 86..526
e-value: 9.0E-213
score: 708.5
NoneNo IPR availablePANTHERPTHR11680:SF34SERINE HYDROXYMETHYLTRANSFERASEcoord: 41..528
IPR019798Serine hydroxymethyltransferase, pyridoxal phosphate binding sitePROSITEPS00096SHMTcoord: 305..321
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 73..527

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G008040.1Lsi07G008040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046655 folic acid metabolic process
biological_process GO:0019264 glycine biosynthetic process from serine
biological_process GO:0006565 L-serine catabolic process
biological_process GO:0032259 methylation
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004372 glycine hydroxymethyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0070905 serine binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity