Lsi07G007270 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi07G007270 vs. ExPASy Swiss-Prot
Match: Q9SF78 (GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana OX=3702 GN=At1g71691 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 4.9e-53
Identity = 138/441 (31.29%), Postives = 194/441 (43.99%), Query Frame = 0

Query: 50  PISPLAPPP-------STSLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSP 109
           PIS  APPP          +VPALFV GDS +D G NN + +FA+A++ PYG DF+   P
Sbjct: 35  PIS--APPPPLVDLNTGDGIVPALFVFGDSLIDNGNNNNIPSFAKANYFPYGIDFN-GGP 94

Query: 110 TGRFCNGRIPVDFLALRLGLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLV 169
           TGRFCNG   VD +A  LGLP +P+Y    G  + +++GVNYASA+AG++  +G   V  
Sbjct: 95  TGRFCNGLTMVDGIAQLLGLPLIPAYSEATG--DQVLRGVNYASAAAGILPDTGGNFV-- 154

Query: 170 FNFNLFYPLIKIIDKYANGTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQ 229
                                                                 I F QQ
Sbjct: 155 ----------------------------------------------------GRIPFDQQ 214

Query: 230 IQQFMDTFQQFVLNMGENAA-ADHISDSVFYISIGINDYIHYYLFNISNVQNLYPPWNFN 289
           I  F  T  Q     G   A AD ++ S+F+I +G NDY++ YL      +N Y    F 
Sbjct: 215 IHNFETTLDQVASKSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFG 274

Query: 290 QFLAATIRQEIKFELLNHFRYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGL 349
             L      ++                                   LYN+  R+ VV GL
Sbjct: 275 DLLVQHYTDQL---------------------------------TRLYNLGGRKFVVAGL 334

Query: 350 APIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGS 409
             +GC P  L Q    +G+C EE+N +V+ FN  ++ M+  L   LP++  I+ D+    
Sbjct: 335 GRMGCIPSILAQ--GNDGKCSEEVNQLVLPFNTNVKTMISNLNQNLPDAKFIYLDIAHMF 380

Query: 410 MDILKNHERYGFNVTSDACCGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAIL 469
            DI+ N   YG       CCG G+  G I C+     C N   +++WD FHPT+ VN I+
Sbjct: 395 EDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNRDQYVFWDAFHPTEKVNLIM 380

Query: 470 ADNVWNSLHTTMCYPKNLQDV 483
           A   +     T+ YP N+Q +
Sbjct: 455 AKKAFAG-DRTVAYPINIQQL 380

BLAST of Lsi07G007270 vs. ExPASy Swiss-Prot
Match: Q9FNP2 (GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.5e-51
Identity = 123/428 (28.74%), Postives = 193/428 (45.09%), Query Frame = 0

Query: 59  STSLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLAL 118
           +T++ PA+FV GDS VD G NN L + AR+++LPYG DF  + PTGRF NG+  VDF+  
Sbjct: 43  TTAMFPAMFVFGDSLVDNGNNNHLNSLARSNYLPYGIDFAGNQPTGRFSNGKTIVDFIGE 102

Query: 119 RLGLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKY 178
            LGLP +P+++       D++ GVNYASA+ G++  +G  L                   
Sbjct: 103 LLGLPEIPAFMDTVDGGVDILHGVNYASAAGGILEETGRHL------------------- 162

Query: 179 ANGTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMG 238
                                              G+  S  +Q++ F  T  +   +M 
Sbjct: 163 -----------------------------------GERFSMGRQVENFEKTLMEISRSMR 222

Query: 239 ENAAADHISDSVFYISIGINDYIHYYLF-NISNVQNLYPPWNFNQFLAATIRQEIKFELL 298
           + +  ++++ S+  +S+G NDYI+ YL   +    ++Y P +F   L +     +     
Sbjct: 223 KESVKEYMAKSLVVVSLGNNDYINNYLKPRLFLSSSIYDPTSFADLLLSNFTTHL----- 282

Query: 299 NHFRYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSE 358
                                       + LY    R+ V+ G+ P+GC P  L    + 
Sbjct: 283 ----------------------------LELYGKGFRKFVIAGVGPLGCIPDQLAAQAAL 342

Query: 359 NGECIEEINDMVMEFNFAMRYMVEELGMELPNSS---IIFCDLLQGSMDILKNHERYGFN 418
            GEC+E +N+M   FN  +  +V+ L  +   +S    ++ +    ++DIL N   YGF 
Sbjct: 343 PGECVEAVNEMAELFNNRLVSLVDRLNSDNKTASEAIFVYGNTYGAAVDILTNPFNYGFE 382

Query: 419 VTSDACCGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMC 478
           VT   CCG GR  G I C+   + C     H++WD FHPT A N I+A   +N   +  C
Sbjct: 403 VTDRGCCGVGRNRGEITCLPLAVPCAFRDRHVFWDAFHPTQAFNLIIALRAFNGSKSD-C 382

Query: 479 YPKNLQDV 483
           YP NL  +
Sbjct: 463 YPINLSQL 382

BLAST of Lsi07G007270 vs. ExPASy Swiss-Prot
Match: Q8LFJ9 (GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.1e-49
Identity = 128/425 (30.12%), Postives = 191/425 (44.94%), Query Frame = 0

Query: 62  LVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG 121
           L PA FV GDS VD G NN++ T ARA++ PYG DF    PTGRFCNGR  VD+ A  LG
Sbjct: 27  LAPAFFVFGDSLVDSGNNNYIPTLARANYFPYGIDFG--FPTGRFCNGRTVVDYGATYLG 86

Query: 122 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKYANG 181
           LP VP YL      ++ ++GVNYASA+AG++  +G                         
Sbjct: 87  LPLVPPYLSPLSIGQNALRGVNYASAAAGILDETGRHY---------------------- 146

Query: 182 TVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMGENA 241
                                           G   +F  QI QF  T +  +    +N 
Sbjct: 147 --------------------------------GARTTFNGQISQFEITIELRLRRFFQNP 206

Query: 242 A--ADHISDSVFYISIGINDYIHYYLF-NISNVQNLYPPWNFNQFLAATIRQEIKFELLN 301
           A    +++ S+  I+IG NDYI+ YL     +    Y   ++   L  T+  +I      
Sbjct: 207 ADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQIS----- 266

Query: 302 HFRYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQY-GSE 361
                                        LYN+ AR++V+ G  P+GC P  L    G+ 
Sbjct: 267 ----------------------------RLYNLGARKMVLAGSGPLGCIPSQLSMVTGNN 326

Query: 362 NGECIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTS 421
              C+ +IN+MV  FN  ++ +   L   LP S  ++ ++     D++ N  RYG  V++
Sbjct: 327 TSGCVTKINNMVSMFNSRLKDLANTLNTTLPGSFFVYQNVFDLFHDMVVNPSRYGLVVSN 361

Query: 422 DACCGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCYPK 481
           +ACCG GRY G + C+     C + + +++WD FHPT+  N I+A N + S      YP 
Sbjct: 387 EACCGNGRYGGALTCLPLQQPCLDRNQYVFWDAFHPTETANKIIAHNTF-SKSANYSYPI 361

Query: 482 NLQDV 483
           ++ ++
Sbjct: 447 SVYEL 361

BLAST of Lsi07G007270 vs. ExPASy Swiss-Prot
Match: Q9C7N5 (GDSL esterase/lipase At1g29660 OS=Arabidopsis thaliana OX=3702 GN=At1g29660 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.5e-46
Identity = 121/423 (28.61%), Postives = 189/423 (44.68%), Query Frame = 0

Query: 63  VPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLGL 122
           VP  F+ GDS VD G NN L + ARAD+ PYG DF    PTGRF NGR  VD L   LG 
Sbjct: 30  VPCYFIFGDSLVDNGNNNRLRSIARADYFPYGIDFG--GPTGRFSNGRTTVDVLTELLGF 89

Query: 123 P-FVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKYANG 182
             ++P+Y   +G  ++++QGVNYASA+AG+   +G++L                      
Sbjct: 90  DNYIPAYSTVSG--QEILQGVNYASAAAGIREETGAQL---------------------- 149

Query: 183 TVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMG-EN 242
                                           GQ I+F+ Q++ + +T  Q V  +G E 
Sbjct: 150 --------------------------------GQRITFSGQVENYKNTVAQVVEILGDEY 209

Query: 243 AAADHISDSVFYISIGINDYI-HYYLFNISNVQNLYPPWNFNQFLAATIRQEIKFELLNH 302
            AAD++   ++ + +G NDY+ +Y++    +    Y P  +   L +  R ++       
Sbjct: 210 TAADYLKRCIYSVGMGSNDYLNNYFMPQFYSTSRQYTPEQYADDLISRYRDQLN------ 269

Query: 303 FRYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSENG 362
                                       LYN  AR+  ++G+  IGC+P  L Q   +  
Sbjct: 270 ---------------------------ALYNYGARKFALVGIGAIGCSPNALAQGSQDGT 329

Query: 363 ECIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTSDA 422
            C+E IN     FN  +  MV++L     ++S  + +      DI+ N   YGF  T+ A
Sbjct: 330 TCVERINSANRIFNNRLISMVQQLNNAHSDASFTYINAYGAFQDIIANPSAYGFTNTNTA 361

Query: 423 CCGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCYPKNL 482
           CCG GR  G + C+     C N   +++WD FHP+ A N  +A   +N+  ++  YP ++
Sbjct: 390 CCGIGRNGGQLTCLPGEPPCLNRDEYVFWDAFHPSAAANTAIAKRSYNAQRSSDVYPIDI 361

BLAST of Lsi07G007270 vs. ExPASy Swiss-Prot
Match: Q9SJB4 (GDSL esterase/lipase At2g04570 OS=Arabidopsis thaliana OX=3702 GN=At2g04570 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.5e-46
Identity = 121/408 (29.66%), Postives = 188/408 (46.08%), Query Frame = 0

Query: 63  VPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLGL 122
           +PA+ V GDS+VD G NN++ T AR++  PYGRDF    PTGRFCNG+I  DF++  LGL
Sbjct: 26  IPAIIVFGDSSVDAGNNNYIPTVARSNFEPYGRDFVGGKPTGRFCNGKIATDFMSEALGL 85

Query: 123 -PFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKYANG 182
            P +P+YL  +  + D   GV +ASA+ G                           Y N 
Sbjct: 86  KPIIPAYLDPSYNISDFATGVTFASAATG---------------------------YDNA 145

Query: 183 TVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMGENA 242
           T             ++++ VL L+         + + + ++ Q  +  +Q      G++ 
Sbjct: 146 T-------------SDVLSVLPLW---------KQLEYYKEYQTKLKAYQ------GKDR 205

Query: 243 AADHISDSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIKFELLNHFR 302
             + I  S++ ISIG ND++  Y F      + Y    +  FLA   ++ +K        
Sbjct: 206 GTETIESSLYLISIGTNDFLENY-FAFPGRSSQYSVSLYQDFLAGIAKEFVK-------- 265

Query: 303 YWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGEC 362
                                     L+ + AR+I + GL P+GC P          GEC
Sbjct: 266 -------------------------KLHGLGARKISLGGLPPMGCMPLERATNIGTGGEC 325

Query: 363 IEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTSDACC 422
           +   ND+ ++FN  +  MVE+L  ELP S+++F +  +  M I+KN   +GF V   ACC
Sbjct: 326 VGRYNDIAVQFNSKLDKMVEKLSKELPGSNLVFSNPYEPFMRIIKNPSSFGFEVVGAACC 344

Query: 423 GFGRYN-GWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNS 469
             G +  G+    +    C NA  +++WD FHPT   N I+A+ + NS
Sbjct: 386 ATGMFEMGYGCQRNNPFTCTNADKYVFWDSFHPTQKTNHIMANALMNS 344

BLAST of Lsi07G007270 vs. ExPASy TrEMBL
Match: A0A6J1EZA3 (GDSL esterase/lipase At1g71691 OS=Cucurbita moschata OX=3662 GN=LOC111437583 PE=3 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 2.4e-204
Identity = 368/486 (75.72%), Postives = 377/486 (77.57%), Query Frame = 0

Query: 1   MAFNLLISLIFIFIPSIFLQSTALLNPRASRNPLAPTPYGFTIPQNSSFPISPLAPPPST 60
           MA NLLI LIFI I SIFL STAL+ P+   NPLAPTPYGFT P NSSFP  P APPPS 
Sbjct: 1   MALNLLIFLIFILISSIFLPSTALIIPKL-HNPLAPTPYGFTTPPNSSFPTFPAAPPPSN 60

Query: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRL 120
           SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTH PTGRFCNGRIPVDFLA RL
Sbjct: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHRPTGRFCNGRIPVDFLAQRL 120

Query: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKYAN 180
           GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSEL                     
Sbjct: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSEL--------------------- 180

Query: 181 GTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMGEN 240
                                            GQHISFT+QIQQFMDTFQQFVL MGEN
Sbjct: 181 ---------------------------------GQHISFTRQIQQFMDTFQQFVLTMGEN 240

Query: 241 AAADHISDSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIKFELLNHF 300
           AAADHISDSVFYISIGINDYIHYYLFNIS VQNLYPPWNFNQFLA TI+QEIK       
Sbjct: 241 AAADHISDSVFYISIGINDYIHYYLFNISRVQNLYPPWNFNQFLATTIKQEIK------- 300

Query: 301 RYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE 360
                                     NLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE
Sbjct: 301 --------------------------NLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE 360

Query: 361 CIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTSDAC 420
           CIEEINDMVMEFNFAMRY+VEELGMELP+ SIIFCDLLQGSMDILKNHERYGFNVTSDAC
Sbjct: 361 CIEEINDMVMEFNFAMRYIVEELGMELPDLSIIFCDLLQGSMDILKNHERYGFNVTSDAC 398

Query: 421 CGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCYPKNLQ 480
           CGFGRYNGWI+CISP+MAC+NASNHIWWDQFHPTDAVNAILADNVWN LHTTMCYPKNLQ
Sbjct: 421 CGFGRYNGWILCISPLMACRNASNHIWWDQFHPTDAVNAILADNVWNGLHTTMCYPKNLQ 398

Query: 481 DVINSQ 487
           DVINSQ
Sbjct: 481 DVINSQ 398

BLAST of Lsi07G007270 vs. ExPASy TrEMBL
Match: A0A6J1JB87 (GDSL esterase/lipase At1g71691 OS=Cucurbita maxima OX=3661 GN=LOC111483416 PE=3 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 1.3e-202
Identity = 365/486 (75.10%), Postives = 375/486 (77.16%), Query Frame = 0

Query: 1   MAFNLLISLIFIFIPSIFLQSTALLNPRASRNPLAPTPYGFTIPQNSSFPISPLAPPPST 60
           MA NLLI LIFI I +IFL STAL+ P+   NPLAPTPYGFT P NSSFP    APPPS 
Sbjct: 1   MALNLLIFLIFILISTIFLPSTALITPKL-HNPLAPTPYGFTSPPNSSFPTLRAAPPPSN 60

Query: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRL 120
           SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTH PTGRFCNGRIPVDFLA RL
Sbjct: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHRPTGRFCNGRIPVDFLAQRL 120

Query: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKYAN 180
           GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSEL                     
Sbjct: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSEL--------------------- 180

Query: 181 GTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMGEN 240
                                            GQHISFT+QIQQFMDTFQQFVL MGEN
Sbjct: 181 ---------------------------------GQHISFTRQIQQFMDTFQQFVLTMGEN 240

Query: 241 AAADHISDSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIKFELLNHF 300
           AAADHISDSVFYISIGINDYIHYYLFNIS VQNLYPPWNFNQFLA TI+QEIK       
Sbjct: 241 AAADHISDSVFYISIGINDYIHYYLFNISRVQNLYPPWNFNQFLATTIKQEIK------- 300

Query: 301 RYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE 360
                                     NLYNMNARRIVVMGLAPIGCAPFYLWQYGS NGE
Sbjct: 301 --------------------------NLYNMNARRIVVMGLAPIGCAPFYLWQYGSVNGE 360

Query: 361 CIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTSDAC 420
           CIEEINDMVMEFNFAMRY+VEELGMELP+ SIIFCDLLQGSMDILKNHERYGFNVTSDAC
Sbjct: 361 CIEEINDMVMEFNFAMRYIVEELGMELPDLSIIFCDLLQGSMDILKNHERYGFNVTSDAC 398

Query: 421 CGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCYPKNLQ 480
           CGFGRYNGWI+CISP+MACKNASNH+WWDQFHPTDAVNAILADNVWN LHTTMCYPKNLQ
Sbjct: 421 CGFGRYNGWILCISPLMACKNASNHVWWDQFHPTDAVNAILADNVWNGLHTTMCYPKNLQ 398

Query: 481 DVINSQ 487
           DVINSQ
Sbjct: 481 DVINSQ 398

BLAST of Lsi07G007270 vs. ExPASy TrEMBL
Match: A0A6J1C4U4 (GDSL esterase/lipase At1g71691 OS=Momordica charantia OX=3673 GN=LOC111008376 PE=3 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 1.2e-198
Identity = 357/483 (73.91%), Postives = 376/483 (77.85%), Query Frame = 0

Query: 1   MAFNLLISLIFIFIPSIFLQSTALLNPRASRNPLAPTPYGFTIPQNSSFPISPLAPPPST 60
           MA NL+I LI I I +IFLQS ALLNP+   +P AP+PYGF  P N+S PISP+APPPS 
Sbjct: 1   MALNLIIPLISITISTIFLQSQALLNPK-DHHPPAPSPYGFISPPNASSPISPVAPPPSN 60

Query: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRL 120
           SLVPALFVIGDSTVD GTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRL
Sbjct: 61  SLVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRL 120

Query: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKYAN 180
           GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSEL                     
Sbjct: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSEL--------------------- 180

Query: 181 GTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMGEN 240
                                            GQHISFTQQIQQFMDTFQQFVL+MGE 
Sbjct: 181 ---------------------------------GQHISFTQQIQQFMDTFQQFVLSMGEK 240

Query: 241 AAADHISDSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIKFELLNHF 300
            AADHIS+SVFYISIGINDYIHYYLFNIS+VQNLY PWNFNQFLAATIRQEIK       
Sbjct: 241 NAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQEIK------- 300

Query: 301 RYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE 360
                                     NLYNMNARRIV+MGLAPIGCAPFYLWQYGSENGE
Sbjct: 301 --------------------------NLYNMNARRIVIMGLAPIGCAPFYLWQYGSENGE 360

Query: 361 CIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTSDAC 420
           CIEEINDMVMEFNFAMRYMVEELGMELP+SSIIFCDLL+GSMDILKNHERYGFNVT+DAC
Sbjct: 361 CIEEINDMVMEFNFAMRYMVEELGMELPDSSIIFCDLLEGSMDILKNHERYGFNVTTDAC 395

Query: 421 CGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCYPKNLQ 480
           CGFGRYNGWIMCISP+MACKNASNHIWWDQFHPTDAVNAILA+NVWN LHTTMCYPKNL+
Sbjct: 421 CGFGRYNGWIMCISPVMACKNASNHIWWDQFHPTDAVNAILAENVWNGLHTTMCYPKNLK 395

Query: 481 DVI 484
           D++
Sbjct: 481 DIL 395

BLAST of Lsi07G007270 vs. ExPASy TrEMBL
Match: A0A0A0L7F0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G270280 PE=3 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 6.3e-197
Identity = 364/488 (74.59%), Postives = 373/488 (76.43%), Query Frame = 0

Query: 1   MAFNLLISLIFIFIPSIFLQSTA-LLNPRASRNPLAPTP-YGFTIPQNSSFPI-SPLAPP 60
           MA +LLISL FI I S+FLQSTA L NP    NPL PTP YGFT   NSSFPI  PL PP
Sbjct: 1   MALSLLISLFFILISSVFLQSTAPLPNPAPHPNPLPPTPNYGFTTIPNSSFPIPPPLPPP 60

Query: 61  PSTSLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLA 120
           PS SLVPALFVIGDSTVD GTNNFLGTFARADHLPYGRDFDTH+PTGRF NGRIPVDFLA
Sbjct: 61  PSNSLVPALFVIGDSTVDSGTNNFLGTFARADHLPYGRDFDTHTPTGRFSNGRIPVDFLA 120

Query: 121 LRLGLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDK 180
           LRLGLPFVPSYLGH GAVEDMIQGVNYASASAGVIFTSGSEL                  
Sbjct: 121 LRLGLPFVPSYLGHVGAVEDMIQGVNYASASAGVIFTSGSEL------------------ 180

Query: 181 YANGTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNM 240
                                               GQHISFTQQIQQFMDTFQQFVLNM
Sbjct: 181 ------------------------------------GQHISFTQQIQQFMDTFQQFVLNM 240

Query: 241 GENAAADHISDSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIKFELL 300
           GE AAADHIS+SVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLA TIRQEIK    
Sbjct: 241 GEKAAADHISNSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAVTIRQEIK---- 300

Query: 301 NHFRYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSE 360
                                        NLYNMNARRIVVMGLAPIGCAPFYLWQY SE
Sbjct: 301 -----------------------------NLYNMNARRIVVMGLAPIGCAPFYLWQYRSE 360

Query: 361 NGECIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTS 420
           NG CIEEINDMVMEFNFAMRY+VEELGMELP+S+IIFCDLLQGSMDILKNHE YGFNVTS
Sbjct: 361 NGACIEEINDMVMEFNFAMRYVVEELGMELPDSNIIFCDLLQGSMDILKNHEYYGFNVTS 401

Query: 421 DACCGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCYPK 480
           +ACCGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWN LHTTMCYPK
Sbjct: 421 NACCGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNGLHTTMCYPK 401

Query: 481 NLQDVINS 486
           NLQDVIN+
Sbjct: 481 NLQDVINA 401

BLAST of Lsi07G007270 vs. ExPASy TrEMBL
Match: A0A5A7UZU1 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold420G00560 PE=3 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 3.5e-195
Identity = 365/490 (74.49%), Postives = 373/490 (76.12%), Query Frame = 0

Query: 1   MAFNLLISLIFIFIPSIFLQS-TALLNPRASRNPLAP-TPY-GFTIPQNSSFPI--SPLA 60
           MA +L ISL FI I S+FLQS T L N     NPLAP TP  GFT   NSSFPI  SPL 
Sbjct: 1   MALSLPISLFFILISSVFLQSATPLPNSAPHPNPLAPSTPNDGFTTIPNSSFPIPPSPLP 60

Query: 61  PPPSTSLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDF 120
           PPPS SLVPALFVIGDSTVD GTNNFLGTFARADHLPYGRDFDTH+PTGRFCNGRIPVDF
Sbjct: 61  PPPSNSLVPALFVIGDSTVDSGTNNFLGTFARADHLPYGRDFDTHTPTGRFCNGRIPVDF 120

Query: 121 LALRLGLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKII 180
           LALRLGLPFVPSYLGH GAVEDMIQGVNYASASAGVIFTSGSEL                
Sbjct: 121 LALRLGLPFVPSYLGHVGAVEDMIQGVNYASASAGVIFTSGSEL---------------- 180

Query: 181 DKYANGTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVL 240
                                                 GQHISFTQQIQQFMDTFQQFVL
Sbjct: 181 --------------------------------------GQHISFTQQIQQFMDTFQQFVL 240

Query: 241 NMGENAAADHISDSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIKFE 300
           NMGE AAADHIS+SVFYISIGINDYIHYYLFNISNVQNL+PPWNFNQFLAATIRQEIK  
Sbjct: 241 NMGEKAAADHISNSVFYISIGINDYIHYYLFNISNVQNLFPPWNFNQFLAATIRQEIK-- 300

Query: 301 LLNHFRYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYG 360
                                          NLYNMNARRIVVMGLAPIGCAPFYLWQY 
Sbjct: 301 -------------------------------NLYNMNARRIVVMGLAPIGCAPFYLWQYR 360

Query: 361 SENGECIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNV 420
           SENG CIEEINDMVMEFNFAMRYMVEELGMELP+SSIIFCDLL+GSMDILKNHE YGFNV
Sbjct: 361 SENGACIEEINDMVMEFNFAMRYMVEELGMELPDSSIIFCDLLEGSMDILKNHEYYGFNV 403

Query: 421 TSDACCGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCY 480
           TS+ACCGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWN LHTTMCY
Sbjct: 421 TSNACCGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNGLHTTMCY 403

Query: 481 PKNLQDVINS 486
           PKNLQDVINS
Sbjct: 481 PKNLQDVINS 403

BLAST of Lsi07G007270 vs. NCBI nr
Match: XP_038904573.1 (GDSL esterase/lipase At1g71691 [Benincasa hispida])

HSP 1 Score: 751.9 bits (1940), Expect = 3.4e-213
Identity = 382/486 (78.60%), Postives = 388/486 (79.84%), Query Frame = 0

Query: 1   MAFNLLISLIFIFIPSIFLQSTALLNPRASRNPLAPTPYGFTIPQNSSFPISPLAPPPST 60
           MA +LLISLIFI I +IFLQSTALLNPRA  NPLAPTPY FT P NSSFPISPLAPPPS 
Sbjct: 1   MALDLLISLIFILISTIFLQSTALLNPRAPPNPLAPTPYAFTTPPNSSFPISPLAPPPSN 60

Query: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRL 120
           SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTH+PTGRFCNGRIPVDFLALRL
Sbjct: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHTPTGRFCNGRIPVDFLALRL 120

Query: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKYAN 180
           GLPFVPSYLGH+GAVEDMIQGVNYASASAGVIFTSGSEL                     
Sbjct: 121 GLPFVPSYLGHSGAVEDMIQGVNYASASAGVIFTSGSEL--------------------- 180

Query: 181 GTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMGEN 240
                                            GQHISFTQQIQQFMDTFQQFVLNMGEN
Sbjct: 181 ---------------------------------GQHISFTQQIQQFMDTFQQFVLNMGEN 240

Query: 241 AAADHISDSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIKFELLNHF 300
           AAADHIS+SVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIK       
Sbjct: 241 AAADHISNSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIK------- 300

Query: 301 RYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE 360
                                     NLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE
Sbjct: 301 --------------------------NLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE 360

Query: 361 CIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTSDAC 420
           CIEEINDMVMEFNFAMRYMVEELGMELP+SSIIFCDLLQGSMDILKNHERYGFNVTSDAC
Sbjct: 361 CIEEINDMVMEFNFAMRYMVEELGMELPDSSIIFCDLLQGSMDILKNHERYGFNVTSDAC 399

Query: 421 CGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCYPKNLQ 480
           CGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVN ILADNVWN LHTTMCYPKNLQ
Sbjct: 421 CGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNGILADNVWNGLHTTMCYPKNLQ 399

Query: 481 DVINSQ 487
           DVINSQ
Sbjct: 481 DVINSQ 399

BLAST of Lsi07G007270 vs. NCBI nr
Match: XP_023552224.1 (GDSL esterase/lipase At1g71691 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 722.6 bits (1864), Expect = 2.2e-204
Identity = 369/486 (75.93%), Postives = 377/486 (77.57%), Query Frame = 0

Query: 1   MAFNLLISLIFIFIPSIFLQSTALLNPRASRNPLAPTPYGFTIPQNSSFPISPLAPPPST 60
           MA NLLI LIFI I SIFL STAL+ P+   NPLAPTPYGFT P NSSFP  P APPPS 
Sbjct: 1   MALNLLIFLIFILISSIFLPSTALIIPKL-HNPLAPTPYGFTTPPNSSFPTFPAAPPPSN 60

Query: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRL 120
           SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTH PTGRFCNGRIPVDFLA RL
Sbjct: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHRPTGRFCNGRIPVDFLAQRL 120

Query: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKYAN 180
           GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSEL                     
Sbjct: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSEL--------------------- 180

Query: 181 GTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMGEN 240
                                            GQHISFT+QIQQFMDTFQQFVL MGEN
Sbjct: 181 ---------------------------------GQHISFTRQIQQFMDTFQQFVLTMGEN 240

Query: 241 AAADHISDSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIKFELLNHF 300
           AAADHISDSVFYISIGINDYIHYYLFNIS VQNLYPPWNFNQFLA TI+QEIK       
Sbjct: 241 AAADHISDSVFYISIGINDYIHYYLFNISRVQNLYPPWNFNQFLATTIKQEIK------- 300

Query: 301 RYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE 360
                                     NLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE
Sbjct: 301 --------------------------NLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE 360

Query: 361 CIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTSDAC 420
           CIEEINDMVMEFNFAMRY+VEELGMELP+ SIIFCDLLQGSMDILKNHERYGFNVTSDAC
Sbjct: 361 CIEEINDMVMEFNFAMRYIVEELGMELPDLSIIFCDLLQGSMDILKNHERYGFNVTSDAC 398

Query: 421 CGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCYPKNLQ 480
           CGFGRYNGWI+CISP+MACKNASNHIWWDQFHPTDAVNAILADNVWN LHTTMCYPKNLQ
Sbjct: 421 CGFGRYNGWILCISPLMACKNASNHIWWDQFHPTDAVNAILADNVWNGLHTTMCYPKNLQ 398

Query: 481 DVINSQ 487
           DVINSQ
Sbjct: 481 DVINSQ 398

BLAST of Lsi07G007270 vs. NCBI nr
Match: XP_022931385.1 (GDSL esterase/lipase At1g71691 [Cucurbita moschata] >KAG7015133.1 GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 721.5 bits (1861), Expect = 5.0e-204
Identity = 368/486 (75.72%), Postives = 377/486 (77.57%), Query Frame = 0

Query: 1   MAFNLLISLIFIFIPSIFLQSTALLNPRASRNPLAPTPYGFTIPQNSSFPISPLAPPPST 60
           MA NLLI LIFI I SIFL STAL+ P+   NPLAPTPYGFT P NSSFP  P APPPS 
Sbjct: 1   MALNLLIFLIFILISSIFLPSTALIIPKL-HNPLAPTPYGFTTPPNSSFPTFPAAPPPSN 60

Query: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRL 120
           SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTH PTGRFCNGRIPVDFLA RL
Sbjct: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHRPTGRFCNGRIPVDFLAQRL 120

Query: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKYAN 180
           GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSEL                     
Sbjct: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSEL--------------------- 180

Query: 181 GTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMGEN 240
                                            GQHISFT+QIQQFMDTFQQFVL MGEN
Sbjct: 181 ---------------------------------GQHISFTRQIQQFMDTFQQFVLTMGEN 240

Query: 241 AAADHISDSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIKFELLNHF 300
           AAADHISDSVFYISIGINDYIHYYLFNIS VQNLYPPWNFNQFLA TI+QEIK       
Sbjct: 241 AAADHISDSVFYISIGINDYIHYYLFNISRVQNLYPPWNFNQFLATTIKQEIK------- 300

Query: 301 RYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE 360
                                     NLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE
Sbjct: 301 --------------------------NLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE 360

Query: 361 CIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTSDAC 420
           CIEEINDMVMEFNFAMRY+VEELGMELP+ SIIFCDLLQGSMDILKNHERYGFNVTSDAC
Sbjct: 361 CIEEINDMVMEFNFAMRYIVEELGMELPDLSIIFCDLLQGSMDILKNHERYGFNVTSDAC 398

Query: 421 CGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCYPKNLQ 480
           CGFGRYNGWI+CISP+MAC+NASNHIWWDQFHPTDAVNAILADNVWN LHTTMCYPKNLQ
Sbjct: 421 CGFGRYNGWILCISPLMACRNASNHIWWDQFHPTDAVNAILADNVWNGLHTTMCYPKNLQ 398

Query: 481 DVINSQ 487
           DVINSQ
Sbjct: 481 DVINSQ 398

BLAST of Lsi07G007270 vs. NCBI nr
Match: XP_022985405.1 (GDSL esterase/lipase At1g71691 [Cucurbita maxima])

HSP 1 Score: 715.7 bits (1846), Expect = 2.7e-202
Identity = 365/486 (75.10%), Postives = 375/486 (77.16%), Query Frame = 0

Query: 1   MAFNLLISLIFIFIPSIFLQSTALLNPRASRNPLAPTPYGFTIPQNSSFPISPLAPPPST 60
           MA NLLI LIFI I +IFL STAL+ P+   NPLAPTPYGFT P NSSFP    APPPS 
Sbjct: 1   MALNLLIFLIFILISTIFLPSTALITPKL-HNPLAPTPYGFTSPPNSSFPTLRAAPPPSN 60

Query: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRL 120
           SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTH PTGRFCNGRIPVDFLA RL
Sbjct: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHRPTGRFCNGRIPVDFLAQRL 120

Query: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKYAN 180
           GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSEL                     
Sbjct: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSEL--------------------- 180

Query: 181 GTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMGEN 240
                                            GQHISFT+QIQQFMDTFQQFVL MGEN
Sbjct: 181 ---------------------------------GQHISFTRQIQQFMDTFQQFVLTMGEN 240

Query: 241 AAADHISDSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIKFELLNHF 300
           AAADHISDSVFYISIGINDYIHYYLFNIS VQNLYPPWNFNQFLA TI+QEIK       
Sbjct: 241 AAADHISDSVFYISIGINDYIHYYLFNISRVQNLYPPWNFNQFLATTIKQEIK------- 300

Query: 301 RYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE 360
                                     NLYNMNARRIVVMGLAPIGCAPFYLWQYGS NGE
Sbjct: 301 --------------------------NLYNMNARRIVVMGLAPIGCAPFYLWQYGSVNGE 360

Query: 361 CIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTSDAC 420
           CIEEINDMVMEFNFAMRY+VEELGMELP+ SIIFCDLLQGSMDILKNHERYGFNVTSDAC
Sbjct: 361 CIEEINDMVMEFNFAMRYIVEELGMELPDLSIIFCDLLQGSMDILKNHERYGFNVTSDAC 398

Query: 421 CGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCYPKNLQ 480
           CGFGRYNGWI+CISP+MACKNASNH+WWDQFHPTDAVNAILADNVWN LHTTMCYPKNLQ
Sbjct: 421 CGFGRYNGWILCISPLMACKNASNHVWWDQFHPTDAVNAILADNVWNGLHTTMCYPKNLQ 398

Query: 481 DVINSQ 487
           DVINSQ
Sbjct: 481 DVINSQ 398

BLAST of Lsi07G007270 vs. NCBI nr
Match: XP_022136745.1 (GDSL esterase/lipase At1g71691 [Momordica charantia])

HSP 1 Score: 702.6 bits (1812), Expect = 2.4e-198
Identity = 357/483 (73.91%), Postives = 376/483 (77.85%), Query Frame = 0

Query: 1   MAFNLLISLIFIFIPSIFLQSTALLNPRASRNPLAPTPYGFTIPQNSSFPISPLAPPPST 60
           MA NL+I LI I I +IFLQS ALLNP+   +P AP+PYGF  P N+S PISP+APPPS 
Sbjct: 1   MALNLIIPLISITISTIFLQSQALLNPK-DHHPPAPSPYGFISPPNASSPISPVAPPPSN 60

Query: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRL 120
           SLVPALFVIGDSTVD GTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRL
Sbjct: 61  SLVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRL 120

Query: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKYAN 180
           GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSEL                     
Sbjct: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSEL--------------------- 180

Query: 181 GTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMGEN 240
                                            GQHISFTQQIQQFMDTFQQFVL+MGE 
Sbjct: 181 ---------------------------------GQHISFTQQIQQFMDTFQQFVLSMGEK 240

Query: 241 AAADHISDSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIKFELLNHF 300
            AADHIS+SVFYISIGINDYIHYYLFNIS+VQNLY PWNFNQFLAATIRQEIK       
Sbjct: 241 NAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQEIK------- 300

Query: 301 RYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE 360
                                     NLYNMNARRIV+MGLAPIGCAPFYLWQYGSENGE
Sbjct: 301 --------------------------NLYNMNARRIVIMGLAPIGCAPFYLWQYGSENGE 360

Query: 361 CIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTSDAC 420
           CIEEINDMVMEFNFAMRYMVEELGMELP+SSIIFCDLL+GSMDILKNHERYGFNVT+DAC
Sbjct: 361 CIEEINDMVMEFNFAMRYMVEELGMELPDSSIIFCDLLEGSMDILKNHERYGFNVTTDAC 395

Query: 421 CGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCYPKNLQ 480
           CGFGRYNGWIMCISP+MACKNASNHIWWDQFHPTDAVNAILA+NVWN LHTTMCYPKNL+
Sbjct: 421 CGFGRYNGWIMCISPVMACKNASNHIWWDQFHPTDAVNAILAENVWNGLHTTMCYPKNLK 395

Query: 481 DVI 484
           D++
Sbjct: 481 DIL 395

BLAST of Lsi07G007270 vs. TAIR 10
Match: AT4G10950.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 488.4 bits (1256), Expect = 6.6e-138
Identity = 254/485 (52.37%), Postives = 305/485 (62.89%), Query Frame = 0

Query: 5   LLISLIFIFI-PSIFLQS--TALLNP-RASRNPLAPTPYGFTIPQNSSFPISPLAPPPST 64
           L+ SL+ IF  P +      T  ++P   S +   P+   F  P  S    SP    PS 
Sbjct: 8   LVFSLLIIFTRPKLIADHHLTTRISPIYPSISTFQPSIPPFLPPSPSRRAQSPTV-KPSL 67

Query: 65  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRL 124
             VPALFV GDS+VD GTNNFLGT ARAD LPYGRDFDTH PTGRFCNGRIPVD+    L
Sbjct: 68  PFVPALFVFGDSSVDSGTNNFLGTLARADRLPYGRDFDTHQPTGRFCNGRIPVDY----L 127

Query: 125 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKYAN 184
           GLPFVPSYLG  G VEDM QGVNYASA AG+I +SGSEL                     
Sbjct: 128 GLPFVPSYLGQTGTVEDMFQGVNYASAGAGIILSSGSEL--------------------- 187

Query: 185 GTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMGEN 244
                                            GQ +SF  Q++QF+DTFQQ +L++GE 
Sbjct: 188 ---------------------------------GQRVSFAMQVEQFVDTFQQMILSIGEK 247

Query: 245 AAADHISDSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIKFELLNHF 304
           A+   +S+SVFYISIG+NDYIH+Y+ NISNVQNLY PWNFNQFLA+ +RQE+K       
Sbjct: 248 ASERLVSNSVFYISIGVNDYIHFYIRNISNVQNLYTPWNFNQFLASNMRQELK------- 307

Query: 305 RYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSENGE 364
                                      LYN+  RR+VVMGL PIGCAP+Y+W+Y S+NGE
Sbjct: 308 --------------------------TLYNVKVRRMVVMGLPPIGCAPYYMWKYRSQNGE 367

Query: 365 CIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTSDAC 424
           C EE+N M+ME NF MRY V++L  ELP +SII+CD+ Q +MDIL+NH+ YGFN T+DAC
Sbjct: 368 CAEEVNSMIMESNFVMRYTVDKLNRELPGASIIYCDVFQSAMDILRNHQHYGFNETTDAC 400

Query: 425 CGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCYPKNLQ 484
           CG GRY GW+ CISP MAC +AS H+WWDQFHPTDAVNAILADNVWN  H  MCYP NL+
Sbjct: 428 CGLGRYKGWLPCISPEMACSDASGHLWWDQFHPTDAVNAILADNVWNGRHVDMCYPTNLE 400

Query: 485 DVINS 486
            +++S
Sbjct: 488 TMLHS 400

BLAST of Lsi07G007270 vs. TAIR 10
Match: AT1G71691.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 210.3 bits (534), Expect = 3.5e-54
Identity = 138/441 (31.29%), Postives = 194/441 (43.99%), Query Frame = 0

Query: 50  PISPLAPPP-------STSLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSP 109
           PIS  APPP          +VPALFV GDS +D G NN + +FA+A++ PYG DF+   P
Sbjct: 35  PIS--APPPPLVDLNTGDGIVPALFVFGDSLIDNGNNNNIPSFAKANYFPYGIDFN-GGP 94

Query: 110 TGRFCNGRIPVDFLALRLGLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLV 169
           TGRFCNG   VD +A  LGLP +P+Y    G  + +++GVNYASA+AG++  +G   V  
Sbjct: 95  TGRFCNGLTMVDGIAQLLGLPLIPAYSEATG--DQVLRGVNYASAAAGILPDTGGNFV-- 154

Query: 170 FNFNLFYPLIKIIDKYANGTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQ 229
                                                                 I F QQ
Sbjct: 155 ----------------------------------------------------GRIPFDQQ 214

Query: 230 IQQFMDTFQQFVLNMGENAA-ADHISDSVFYISIGINDYIHYYLFNISNVQNLYPPWNFN 289
           I  F  T  Q     G   A AD ++ S+F+I +G NDY++ YL      +N Y    F 
Sbjct: 215 IHNFETTLDQVASKSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFG 274

Query: 290 QFLAATIRQEIKFELLNHFRYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGL 349
             L      ++                                   LYN+  R+ VV GL
Sbjct: 275 DLLVQHYTDQL---------------------------------TRLYNLGGRKFVVAGL 334

Query: 350 APIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGS 409
             +GC P  L Q    +G+C EE+N +V+ FN  ++ M+  L   LP++  I+ D+    
Sbjct: 335 GRMGCIPSILAQ--GNDGKCSEEVNQLVLPFNTNVKTMISNLNQNLPDAKFIYLDIAHMF 380

Query: 410 MDILKNHERYGFNVTSDACCGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAIL 469
            DI+ N   YG       CCG G+  G I C+     C N   +++WD FHPT+ VN I+
Sbjct: 395 EDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNRDQYVFWDAFHPTEKVNLIM 380

Query: 470 ADNVWNSLHTTMCYPKNLQDV 483
           A   +     T+ YP N+Q +
Sbjct: 455 AKKAFAG-DRTVAYPINIQQL 380

BLAST of Lsi07G007270 vs. TAIR 10
Match: AT5G08460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 204.1 bits (518), Expect = 2.5e-52
Identity = 123/428 (28.74%), Postives = 193/428 (45.09%), Query Frame = 0

Query: 59  STSLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLAL 118
           +T++ PA+FV GDS VD G NN L + AR+++LPYG DF  + PTGRF NG+  VDF+  
Sbjct: 43  TTAMFPAMFVFGDSLVDNGNNNHLNSLARSNYLPYGIDFAGNQPTGRFSNGKTIVDFIGE 102

Query: 119 RLGLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKY 178
            LGLP +P+++       D++ GVNYASA+ G++  +G  L                   
Sbjct: 103 LLGLPEIPAFMDTVDGGVDILHGVNYASAAGGILEETGRHL------------------- 162

Query: 179 ANGTVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMG 238
                                              G+  S  +Q++ F  T  +   +M 
Sbjct: 163 -----------------------------------GERFSMGRQVENFEKTLMEISRSMR 222

Query: 239 ENAAADHISDSVFYISIGINDYIHYYLF-NISNVQNLYPPWNFNQFLAATIRQEIKFELL 298
           + +  ++++ S+  +S+G NDYI+ YL   +    ++Y P +F   L +     +     
Sbjct: 223 KESVKEYMAKSLVVVSLGNNDYINNYLKPRLFLSSSIYDPTSFADLLLSNFTTHL----- 282

Query: 299 NHFRYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSE 358
                                       + LY    R+ V+ G+ P+GC P  L    + 
Sbjct: 283 ----------------------------LELYGKGFRKFVIAGVGPLGCIPDQLAAQAAL 342

Query: 359 NGECIEEINDMVMEFNFAMRYMVEELGMELPNSS---IIFCDLLQGSMDILKNHERYGFN 418
            GEC+E +N+M   FN  +  +V+ L  +   +S    ++ +    ++DIL N   YGF 
Sbjct: 343 PGECVEAVNEMAELFNNRLVSLVDRLNSDNKTASEAIFVYGNTYGAAVDILTNPFNYGFE 382

Query: 419 VTSDACCGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMC 478
           VT   CCG GR  G I C+   + C     H++WD FHPT A N I+A   +N   +  C
Sbjct: 403 VTDRGCCGVGRNRGEITCLPLAVPCAFRDRHVFWDAFHPTQAFNLIIALRAFNGSKSD-C 382

Query: 479 YPKNLQDV 483
           YP NL  +
Sbjct: 463 YPINLSQL 382

BLAST of Lsi07G007270 vs. TAIR 10
Match: AT5G15720.1 (GDSL-motif lipase 7 )

HSP 1 Score: 199.1 bits (505), Expect = 8.0e-51
Identity = 128/425 (30.12%), Postives = 191/425 (44.94%), Query Frame = 0

Query: 62  LVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG 121
           L PA FV GDS VD G NN++ T ARA++ PYG DF    PTGRFCNGR  VD+ A  LG
Sbjct: 27  LAPAFFVFGDSLVDSGNNNYIPTLARANYFPYGIDFG--FPTGRFCNGRTVVDYGATYLG 86

Query: 122 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKYANG 181
           LP VP YL      ++ ++GVNYASA+AG++  +G                         
Sbjct: 87  LPLVPPYLSPLSIGQNALRGVNYASAAAGILDETGRHY---------------------- 146

Query: 182 TVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMGENA 241
                                           G   +F  QI QF  T +  +    +N 
Sbjct: 147 --------------------------------GARTTFNGQISQFEITIELRLRRFFQNP 206

Query: 242 A--ADHISDSVFYISIGINDYIHYYLF-NISNVQNLYPPWNFNQFLAATIRQEIKFELLN 301
           A    +++ S+  I+IG NDYI+ YL     +    Y   ++   L  T+  +I      
Sbjct: 207 ADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQIS----- 266

Query: 302 HFRYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQY-GSE 361
                                        LYN+ AR++V+ G  P+GC P  L    G+ 
Sbjct: 267 ----------------------------RLYNLGARKMVLAGSGPLGCIPSQLSMVTGNN 326

Query: 362 NGECIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTS 421
              C+ +IN+MV  FN  ++ +   L   LP S  ++ ++     D++ N  RYG  V++
Sbjct: 327 TSGCVTKINNMVSMFNSRLKDLANTLNTTLPGSFFVYQNVFDLFHDMVVNPSRYGLVVSN 361

Query: 422 DACCGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCYPK 481
           +ACCG GRY G + C+     C + + +++WD FHPT+  N I+A N + S      YP 
Sbjct: 387 EACCGNGRYGGALTCLPLQQPCLDRNQYVFWDAFHPTETANKIIAHNTF-SKSANYSYPI 361

Query: 482 NLQDV 483
           ++ ++
Sbjct: 447 SVYEL 361

BLAST of Lsi07G007270 vs. TAIR 10
Match: AT1G29660.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 188.7 bits (478), Expect = 1.1e-47
Identity = 121/423 (28.61%), Postives = 189/423 (44.68%), Query Frame = 0

Query: 63  VPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLGL 122
           VP  F+ GDS VD G NN L + ARAD+ PYG DF    PTGRF NGR  VD L   LG 
Sbjct: 30  VPCYFIFGDSLVDNGNNNRLRSIARADYFPYGIDFG--GPTGRFSNGRTTVDVLTELLGF 89

Query: 123 P-FVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELVLVFNFNLFYPLIKIIDKYANG 182
             ++P+Y   +G  ++++QGVNYASA+AG+   +G++L                      
Sbjct: 90  DNYIPAYSTVSG--QEILQGVNYASAAAGIREETGAQL---------------------- 149

Query: 183 TVSNQIDFYSFQQFNNMIVVLSLFALSAKDMEGQHISFTQQIQQFMDTFQQFVLNMG-EN 242
                                           GQ I+F+ Q++ + +T  Q V  +G E 
Sbjct: 150 --------------------------------GQRITFSGQVENYKNTVAQVVEILGDEY 209

Query: 243 AAADHISDSVFYISIGINDYI-HYYLFNISNVQNLYPPWNFNQFLAATIRQEIKFELLNH 302
            AAD++   ++ + +G NDY+ +Y++    +    Y P  +   L +  R ++       
Sbjct: 210 TAADYLKRCIYSVGMGSNDYLNNYFMPQFYSTSRQYTPEQYADDLISRYRDQLN------ 269

Query: 303 FRYWTLTLYCVLGAAPALHRSSINLSINLYNMNARRIVVMGLAPIGCAPFYLWQYGSENG 362
                                       LYN  AR+  ++G+  IGC+P  L Q   +  
Sbjct: 270 ---------------------------ALYNYGARKFALVGIGAIGCSPNALAQGSQDGT 329

Query: 363 ECIEEINDMVMEFNFAMRYMVEELGMELPNSSIIFCDLLQGSMDILKNHERYGFNVTSDA 422
            C+E IN     FN  +  MV++L     ++S  + +      DI+ N   YGF  T+ A
Sbjct: 330 TCVERINSANRIFNNRLISMVQQLNNAHSDASFTYINAYGAFQDIIANPSAYGFTNTNTA 361

Query: 423 CCGFGRYNGWIMCISPIMACKNASNHIWWDQFHPTDAVNAILADNVWNSLHTTMCYPKNL 482
           CCG GR  G + C+     C N   +++WD FHP+ A N  +A   +N+  ++  YP ++
Sbjct: 390 CCGIGRNGGQLTCLPGEPPCLNRDEYVFWDAFHPSAAANTAIAKRSYNAQRSSDVYPIDI 361