Lsi07G006290 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi07G006290
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	D-3-phosphoglycerate dehydrogenase
Location	chr07: 6739162 .. 6739809 (-)
RNA-Seq Expression	Lsi07G006290
Synteny	Lsi07G006290

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCTCTTCTGCAAAATCCCTCTTTTCCTCTCCCCATTTCACCTCATTCAACAGATCCTCCCATTCCAAATCCCCTCTTCTCTCCTTTTCCAAATCCTCTGTTTCTCTCAATCTTCACCCTCTTCATTCTTCTTATTCTTCTTCTTCTTCTTCTAACCGCTGCAATTTCATTCCACTTCGTAGCGTTTTGAAAACTGTTGAATCCTCCGAATTAGAGGTTAAGAACTCTGATCTCGTCGACCCCACTTTCCCTAATGACAGATCCACCATTTTGGTGGCCGAGAAACTTGGAGAGGCCGGTCTTAAGCTTCTCCGGAGCTTTGGGAACGTTGAGTGTGCTTACGACCTCTCGCCGGAAGATCTTTGCGCCAAGATCTCTTCGTGCGACGCTTTGATTGTCCGAAGTGGCACCAAGGTGAACAGGCAGGTCTTTGAGGCTGCAAAAGGGCGTTTGAAAGTTGTTGGAAGAGCCGGCGTTGGTATTGACAATGTGGATCTGCAGGCCGCCACTGAGTTCGGTTGTCTCGTTGTTAATGCCCCAACTGCCAACACCATCGCCGCCGCCGAGCATGGAATTGCTTTGCTTACTGCCATGGCCAGAAACGTCGCTCAGGCCGATGCCTCCATGAAAGCAGGTCGGTAA

mRNA sequence

ATGGCTTCCTCTTCTGCAAAATCCCTCTTTTCCTCTCCCCATTTCACCTCATTCAACAGATCCTCCCATTCCAAATCCCCTCTTCTCTCCTTTTCCAAATCCTCTGTTTCTCTCAATCTTCACCCTCTTCATTCTTCTTATTCTTCTTCTTCTTCTTCTAACCGCTGCAATTTCATTCCACTTCGTAGCGTTTTGAAAACTGTTGAATCCTCCGAATTAGAGGTTAAGAACTCTGATCTCGTCGACCCCACTTTCCCTAATGACAGATCCACCATTTTGGTGGCCGAGAAACTTGGAGAGGCCGGTCTTAAGCTTCTCCGGAGCTTTGGGAACGTTGAGTGTGCTTACGACCTCTCGCCGGAAGATCTTTGCGCCAAGATCTCTTCGTGCGACGCTTTGATTGTCCGAAGTGGCACCAAGGTGAACAGGCAGGTCTTTGAGGCTGCAAAAGGGCGTTTGAAAGTTGTTGGAAGAGCCGGCGTTGGTATTGACAATGTGGATCTGCAGGCCGCCACTGAGTTCGGTTGTCTCGTTGTTAATGCCCCAACTGCCAACACCATCGCCGCCGCCGAGCATGGAATTGCTTTGCTTACTGCCATGGCCAGAAACGTCGCTCAGGCCGATGCCTCCATGAAAGCAGGTCGGTAA

Coding sequence (CDS)

ATGGCTTCCTCTTCTGCAAAATCCCTCTTTTCCTCTCCCCATTTCACCTCATTCAACAGATCCTCCCATTCCAAATCCCCTCTTCTCTCCTTTTCCAAATCCTCTGTTTCTCTCAATCTTCACCCTCTTCATTCTTCTTATTCTTCTTCTTCTTCTTCTAACCGCTGCAATTTCATTCCACTTCGTAGCGTTTTGAAAACTGTTGAATCCTCCGAATTAGAGGTTAAGAACTCTGATCTCGTCGACCCCACTTTCCCTAATGACAGATCCACCATTTTGGTGGCCGAGAAACTTGGAGAGGCCGGTCTTAAGCTTCTCCGGAGCTTTGGGAACGTTGAGTGTGCTTACGACCTCTCGCCGGAAGATCTTTGCGCCAAGATCTCTTCGTGCGACGCTTTGATTGTCCGAAGTGGCACCAAGGTGAACAGGCAGGTCTTTGAGGCTGCAAAAGGGCGTTTGAAAGTTGTTGGAAGAGCCGGCGTTGGTATTGACAATGTGGATCTGCAGGCCGCCACTGAGTTCGGTTGTCTCGTTGTTAATGCCCCAACTGCCAACACCATCGCCGCCGCCGAGCATGGAATTGCTTTGCTTACTGCCATGGCCAGAAACGTCGCTCAGGCCGATGCCTCCATGAAAGCAGGTCGGTAA

Protein sequence

MASSSAKSLFSSPHFTSFNRSSHSKSPLLSFSKSSVSLNLHPLHSSYSSSSSSNRCNFIPLRSVLKTVESSELEVKNSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVECAYDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGR

Homology

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BLAST of Lsi07G006290 vs. ExPASy Swiss-Prot
Match: O04130 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH2 PE=1 SV=2)

HSP 1 Score: 211.5 bits (537), Expect = 9.7e-54
Identity = 120/181 (66.30%), Postives = 137/181 (75.69%), Query Frame = 0


Query: 43  LHSSYSSSSSSNRCNFIPLRSVLKTVESSELEVKN--------SDLVDPTFPNDRSTILV 102
           LH  Y++S          + + LKTVE + L   N        SD  +PT P  R  ILV
Sbjct: 35  LHRRYATS-----VKLTAISAALKTVEQTTLTEDNRFSTVGSDSDEYNPTLPKPR--ILV 94

Query: 103 AEKLGEAGLKLLRSFGNVECAYDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLK 162
            EKLGEAG+ LLR FG+V+C+YDLSPEDL  K++  DALIVRSGTKV R+VFEAAKGRLK
Sbjct: 95  TEKLGEAGVNLLREFGDVDCSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEAAKGRLK 154

Query: 163 VVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAG 216
           VVGRAGVGIDNVDLQAATE GCLVVNAPTANT+AAAEHGIALL +MARNVAQADAS+KAG
Sbjct: 155 VVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASMARNVAQADASIKAG 208

[Close]

BLAST of Lsi07G006290 vs. ExPASy Swiss-Prot
Match: Q9LT69 (D-3-phosphoglycerate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH3 PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 1.9e-49
Identity = 114/185 (61.62%), Postives = 136/185 (73.51%), Query Frame = 0


Query: 35  SVSLNLHPLHSSYSSSSSSNRCNFIPLRS----VLKTVESSELEVKNSDLVDPTFPNDRS 94
           + SLNL  + SS SS   +   +  P+R     +L T  SS                 + 
Sbjct: 2   ATSLNLSSIFSS-SSRLVTTPSSVFPIRQRRRIILVTSSSSG-------------GGGKP 61

Query: 95  TILVAEKLGEAGLKLLRSFGNVECAYDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAK 154
           TILV EKLG+AG+ LL+ + NV+C+YDLS E+LC KIS CDALIVRSGTKV R VFE+++
Sbjct: 62  TILVTEKLGQAGIDLLKKYANVDCSYDLSLEELCTKISLCDALIVRSGTKVGRDVFESSR 121

Query: 155 GRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADAS 214
           GRLKVVGRAGVGIDNVDL AATE+GCLVVNAPTANT+AAAEHGIALLTAMARN+AQADAS
Sbjct: 122 GRLKVVGRAGVGIDNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADAS 172

Query: 215 MKAGR 216
           +KAG+
Sbjct: 182 IKAGK 172

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BLAST of Lsi07G006290 vs. ExPASy Swiss-Prot
Match: O49485 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH1 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.1e-48
Identity = 109/173 (63.01%), Postives = 129/173 (74.57%), Query Frame = 0


Query: 42  PLHSSYSSSSSSNRCNFIPLRSVLKTVESSELEVKNSDLVDPTFPNDRSTILVAEKLGEA 101
           PL S+ S +  S   N +  R VL +  + +                + TILVAEKLG+A
Sbjct: 28  PLPSAISVAFPSRGRNTLQRRLVLVSCSTGD--------------GSKPTILVAEKLGDA 87

Query: 102 GLKLLRSFGNVECAYDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGV 161
           G+KLL    NV+C+Y+++PE+L  KIS CDALIVRSGTKV R+VFE++ GRLKVVGRAGV
Sbjct: 88  GIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGREVFESSHGRLKVVGRAGV 147

Query: 162 GIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAG 215
           GIDNVDL AATEFGCLVVNAPTANTIAAAEHGIAL+ AMARNVAQADAS+KAG
Sbjct: 148 GIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADASVKAG 186

[Close]

BLAST of Lsi07G006290 vs. ExPASy Swiss-Prot
Match: Q58424 (D-3-phosphoglycerate dehydrogenase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=serA PE=3 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.7e-26
Identity = 66/123 (53.66%), Postives = 87/123 (70.73%), Query Frame = 0


Query: 92  ILVAEKLGEAGLKLLRSFGNVECAYDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAKG 151
           ILV + L E  +K+L   G VE A  L+ E+L  KI   D L+VRSGTKV R V E A+ 
Sbjct: 4   ILVTDPLHEDAIKILEEVGEVEVATGLTKEELLEKIKDADVLVVRSGTKVTRDVIEKAE- 63

Query: 152 RLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASM 211
           +LKV+GRAGVG+DN+D++AATE G +VVNAP A++I+ AE  + L+ A ARN+ QA AS+
Sbjct: 64  KLKVIGRAGVGVDNIDVEAATEKGIIVVNAPDASSISVAELTMGLMLAAARNIPQATASL 123

Query: 212 KAG 215
           K G
Sbjct: 124 KRG 125

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BLAST of Lsi07G006290 vs. ExPASy Swiss-Prot
Match: O29445 (D-3-phosphoglycerate dehydrogenase OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=serA PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.6e-24
Identity = 61/125 (48.80%), Postives = 87/125 (69.60%), Query Frame = 0


Query: 92  ILVAEKLGEAGLKLLRSFG-NVECAYDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAK 151
           +LVAE + E  +  +R  G  VE    +S E+L  ++   +A++VRS TKV+ +V +AAK
Sbjct: 3   VLVAEPISEEAIDYMRKNGLEVEVKTGMSREELIREVPKYEAIVVRSQTKVDAEVIQAAK 62

Query: 152 GRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADAS 211
             LK++GRAGVG+DN+D+ AAT+ G +VVNAP  NTI+ AEH IAL+ A AR + QAD S
Sbjct: 63  -NLKIIGRAGVGVDNIDINAATQRGIVVVNAPGGNTISTAEHAIALMLAAARKIPQADRS 122

Query: 212 MKAGR 216
           +K G+
Sbjct: 123 VKEGK 126

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BLAST of Lsi07G006290 vs. ExPASy TrEMBL
Match: A0A5A7VDA4 (D-3-phosphoglycerate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold90G00680 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 5.3e-95
Identity = 197/216 (91.20%), Postives = 205/216 (94.91%), Query Frame = 0


Query: 1   MASSSAKSLFSSPHFTSFNRSSHSKSPLLSFSKSSVSLNLHPLHSSY-SSSSSSNRCNFI 60
           MASSS+KSLFSSPHFTSFNRSSHSKSPLLSFS SSVSLN  PLHSS  SSSSSSNRC+FI
Sbjct: 1   MASSSSKSLFSSPHFTSFNRSSHSKSPLLSFSTSSVSLNPRPLHSSITSSSSSSNRCSFI 60

Query: 61  PLRSVLKTVESSELEVKNSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVECAYDLS 120
           PLRSVLKT+ESS    +NSDLVDPT PNDRSTILVAEKLGEAGL+LLRSFGNV CAYDLS
Sbjct: 61  PLRSVLKTLESSAFVSQNSDLVDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCAYDLS 120

Query: 121 PEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180
           PE+LCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV
Sbjct: 121 PEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180

Query: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGR 216
           NAPTANTIAAAEHGIALLTAMARNVAQADAS+KAG+
Sbjct: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASIKAGK 216

[Close]

BLAST of Lsi07G006290 vs. ExPASy TrEMBL
Match: A0A1S3CKG7 (D-3-phosphoglycerate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103501977 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 5.3e-95
Identity = 197/216 (91.20%), Postives = 205/216 (94.91%), Query Frame = 0


Query: 1   MASSSAKSLFSSPHFTSFNRSSHSKSPLLSFSKSSVSLNLHPLHSSY-SSSSSSNRCNFI 60
           MASSS+KSLFSSPHFTSFNRSSHSKSPLLSFS SSVSLN  PLHSS  SSSSSSNRC+FI
Sbjct: 1   MASSSSKSLFSSPHFTSFNRSSHSKSPLLSFSTSSVSLNPRPLHSSITSSSSSSNRCSFI 60

Query: 61  PLRSVLKTVESSELEVKNSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVECAYDLS 120
           PLRSVLKT+ESS    +NSDLVDPT PNDRSTILVAEKLGEAGL+LLRSFGNV CAYDLS
Sbjct: 61  PLRSVLKTLESSAFVSQNSDLVDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCAYDLS 120

Query: 121 PEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180
           PE+LCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV
Sbjct: 121 PEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180

Query: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGR 216
           NAPTANTIAAAEHGIALLTAMARNVAQADAS+KAG+
Sbjct: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASIKAGK 216

[Close]

BLAST of Lsi07G006290 vs. ExPASy TrEMBL
Match: A0A5D3CA37 (D-3-phosphoglycerate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold606G00710 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 2.6e-94
Identity = 196/220 (89.09%), Postives = 205/220 (93.18%), Query Frame = 0


Query: 1   MASSSAKSLFSSPHFTSFNRSSHSKSPLLSFSKSSVSLNLHPLHSSY-----SSSSSSNR 60
           MASSS+KS+FSSPHFTSFNRSSHSKSPLLSFS SSVSLN  PLHSS      SSSSSSNR
Sbjct: 1   MASSSSKSIFSSPHFTSFNRSSHSKSPLLSFSTSSVSLNPRPLHSSITSSSSSSSSSSNR 60

Query: 61  CNFIPLRSVLKTVESSELEVKNSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVECA 120
           C+FIPLRSVLKT+ESS    +NSDLVDPT PNDRSTILVAEKLGEAGL+LLRSFGNV CA
Sbjct: 61  CSFIPLRSVLKTLESSAFVSQNSDLVDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 120

Query: 121 YDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180
           YDLSPE+LCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180

Query: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGR 216
           CLVVNAPTANTIAAAEHGIALLTAMARNVAQADAS+KAG+
Sbjct: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASIKAGK 220

[Close]

BLAST of Lsi07G006290 vs. ExPASy TrEMBL
Match: A0A0A0LC35 (D-3-phosphoglycerate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_3G651740 PE=3 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 8.4e-93
Identity = 193/216 (89.35%), Postives = 202/216 (93.52%), Query Frame = 0


Query: 1   MASSSAKSLFSSPHFTSFNRSSHSKSPLLSFSKSSVSLNLHPLH-SSYSSSSSSNRCNFI 60
           MASSS+ SLFSSPHFTSFNRS HSK PLLSFS SSVSLN  PLH SS SSSSSSNRC+FI
Sbjct: 1   MASSSSNSLFSSPHFTSFNRSFHSKPPLLSFSTSSVSLNPRPLHSSSSSSSSSSNRCSFI 60

Query: 61  PLRSVLKTVESSELEVKNSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVECAYDLS 120
           PLRSVLKT+ESS    +NSDL+DPT PNDRSTILVAEKLGEAGL+LLRSFGNV CAYDLS
Sbjct: 61  PLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCAYDLS 120

Query: 121 PEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180
           PE+LCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV
Sbjct: 121 PEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180

Query: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGR 216
           NAPTANTIAAAEHGIALLT+MARNVAQADASMKAG+
Sbjct: 181 NAPTANTIAAAEHGIALLTSMARNVAQADASMKAGK 216

[Close]

BLAST of Lsi07G006290 vs. ExPASy TrEMBL
Match: A0A6J1IWT4 (D-3-phosphoglycerate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111479899 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.5e-84
Identity = 184/220 (83.64%), Postives = 195/220 (88.64%), Query Frame = 0


Query: 1   MASSSAKSLFSSPHFTSFNRS----SHSKSPLLSFSKSSVSLNLHPLHSSYSSSSSSNR- 60
           MASSSAKSLFSS HF SF+RS    S+S SP+L F           LHSS S+SS S R 
Sbjct: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPF-----------LHSSISTSSVSLRS 60

Query: 61  CNFIPLRSVLKTVESSELEVKNSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVECA 120
            N IP+RSVLKTVESSEL+V++SDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNV+CA
Sbjct: 61  SNLIPVRSVLKTVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCA 120

Query: 121 YDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180
           YDLSPE+LCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180

Query: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGR 216
           CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAG+
Sbjct: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGK 209

[Close]

BLAST of Lsi07G006290 vs. NCBI nr
Match: XP_038895985.1 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 380.9 bits (977), Expect = 7.0e-102
Identity = 209/216 (96.76%), Postives = 211/216 (97.69%), Query Frame = 0


Query: 1   MASSSAKSLFSSPHFTSFNRSSHSKSPLLSFSKSSVSLNLHPLHSSY-SSSSSSNRCNFI 60
           MASSSAKSLFSSPHFTSFNRSSHSKSPLLSFS SSVSLNLHPLHSS  SSSSSSNRCNFI
Sbjct: 1   MASSSAKSLFSSPHFTSFNRSSHSKSPLLSFSNSSVSLNLHPLHSSSPSSSSSSNRCNFI 60

Query: 61  PLRSVLKTVESSELEVKNSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVECAYDLS 120
           PLRSVLKTVESS LEV+NSDLV PTFPNDRSTILVAEKLGEAGLKLLRSFGNVECAYDLS
Sbjct: 61  PLRSVLKTVESSGLEVQNSDLVGPTFPNDRSTILVAEKLGEAGLKLLRSFGNVECAYDLS 120

Query: 121 PEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180
           PEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV
Sbjct: 121 PEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180

Query: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGR 216
           NAPTANTIAAAEHGIALLTAMARNVAQADASMKAG+
Sbjct: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGK 216

[Close]

BLAST of Lsi07G006290 vs. NCBI nr
Match: KAA0065618.1 (D-3-phosphoglycerate dehydrogenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 357.1 bits (915), Expect = 1.1e-94
Identity = 197/216 (91.20%), Postives = 205/216 (94.91%), Query Frame = 0


Query: 1   MASSSAKSLFSSPHFTSFNRSSHSKSPLLSFSKSSVSLNLHPLHSSY-SSSSSSNRCNFI 60
           MASSS+KSLFSSPHFTSFNRSSHSKSPLLSFS SSVSLN  PLHSS  SSSSSSNRC+FI
Sbjct: 1   MASSSSKSLFSSPHFTSFNRSSHSKSPLLSFSTSSVSLNPRPLHSSITSSSSSSNRCSFI 60

Query: 61  PLRSVLKTVESSELEVKNSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVECAYDLS 120
           PLRSVLKT+ESS    +NSDLVDPT PNDRSTILVAEKLGEAGL+LLRSFGNV CAYDLS
Sbjct: 61  PLRSVLKTLESSAFVSQNSDLVDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCAYDLS 120

Query: 121 PEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180
           PE+LCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV
Sbjct: 121 PEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180

Query: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGR 216
           NAPTANTIAAAEHGIALLTAMARNVAQADAS+KAG+
Sbjct: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASIKAGK 216

[Close]

BLAST of Lsi07G006290 vs. NCBI nr
Match: XP_008463985.1 (PREDICTED: D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucumis melo])

HSP 1 Score: 357.1 bits (915), Expect = 1.1e-94
Identity = 197/216 (91.20%), Postives = 205/216 (94.91%), Query Frame = 0


Query: 1   MASSSAKSLFSSPHFTSFNRSSHSKSPLLSFSKSSVSLNLHPLHSSY-SSSSSSNRCNFI 60
           MASSS+KSLFSSPHFTSFNRSSHSKSPLLSFS SSVSLN  PLHSS  SSSSSSNRC+FI
Sbjct: 1   MASSSSKSLFSSPHFTSFNRSSHSKSPLLSFSTSSVSLNPRPLHSSITSSSSSSNRCSFI 60

Query: 61  PLRSVLKTVESSELEVKNSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVECAYDLS 120
           PLRSVLKT+ESS    +NSDLVDPT PNDRSTILVAEKLGEAGL+LLRSFGNV CAYDLS
Sbjct: 61  PLRSVLKTLESSAFVSQNSDLVDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCAYDLS 120

Query: 121 PEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180
           PE+LCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV
Sbjct: 121 PEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180

Query: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGR 216
           NAPTANTIAAAEHGIALLTAMARNVAQADAS+KAG+
Sbjct: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASIKAGK 216

[Close]

BLAST of Lsi07G006290 vs. NCBI nr
Match: TYK07186.1 (D-3-phosphoglycerate dehydrogenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 354.8 bits (909), Expect = 5.4e-94
Identity = 196/220 (89.09%), Postives = 205/220 (93.18%), Query Frame = 0


Query: 1   MASSSAKSLFSSPHFTSFNRSSHSKSPLLSFSKSSVSLNLHPLHSSY-----SSSSSSNR 60
           MASSS+KS+FSSPHFTSFNRSSHSKSPLLSFS SSVSLN  PLHSS      SSSSSSNR
Sbjct: 1   MASSSSKSIFSSPHFTSFNRSSHSKSPLLSFSTSSVSLNPRPLHSSITSSSSSSSSSSNR 60

Query: 61  CNFIPLRSVLKTVESSELEVKNSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVECA 120
           C+FIPLRSVLKT+ESS    +NSDLVDPT PNDRSTILVAEKLGEAGL+LLRSFGNV CA
Sbjct: 61  CSFIPLRSVLKTLESSAFVSQNSDLVDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 120

Query: 121 YDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180
           YDLSPE+LCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180

Query: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGR 216
           CLVVNAPTANTIAAAEHGIALLTAMARNVAQADAS+KAG+
Sbjct: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASIKAGK 220

[Close]

BLAST of Lsi07G006290 vs. NCBI nr
Match: XP_022980570.1 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 321.6 bits (823), Expect = 5.1e-84
Identity = 184/220 (83.64%), Postives = 195/220 (88.64%), Query Frame = 0


Query: 1   MASSSAKSLFSSPHFTSFNRS----SHSKSPLLSFSKSSVSLNLHPLHSSYSSSSSSNR- 60
           MASSSAKSLFSS HF SF+RS    S+S SP+L F           LHSS S+SS S R 
Sbjct: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPF-----------LHSSISTSSVSLRS 60

Query: 61  CNFIPLRSVLKTVESSELEVKNSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVECA 120
            N IP+RSVLKTVESSEL+V++SDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNV+CA
Sbjct: 61  SNLIPVRSVLKTVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCA 120

Query: 121 YDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180
           YDLSPE+LCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180

Query: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGR 216
           CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAG+
Sbjct: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGK 209

[Close]

BLAST of Lsi07G006290 vs. TAIR 10
Match: AT1G17745.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 211.5 bits (537), Expect = 6.9e-55
Identity = 120/181 (66.30%), Postives = 137/181 (75.69%), Query Frame = 0


Query: 43  LHSSYSSSSSSNRCNFIPLRSVLKTVESSELEVKN--------SDLVDPTFPNDRSTILV 102
           LH  Y++S          + + LKTVE + L   N        SD  +PT P  R  ILV
Sbjct: 35  LHRRYATS-----VKLTAISAALKTVEQTTLTEDNRFSTVGSDSDEYNPTLPKPR--ILV 94

Query: 103 AEKLGEAGLKLLRSFGNVECAYDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLK 162
            EKLGEAG+ LLR FG+V+C+YDLSPEDL  K++  DALIVRSGTKV R+VFEAAKGRLK
Sbjct: 95  TEKLGEAGVNLLREFGDVDCSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEAAKGRLK 154

Query: 163 VVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAG 216
           VVGRAGVGIDNVDLQAATE GCLVVNAPTANT+AAAEHGIALL +MARNVAQADAS+KAG
Sbjct: 155 VVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASMARNVAQADASIKAG 208

[Close]

BLAST of Lsi07G006290 vs. TAIR 10
Match: AT1G17745.2 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 210.7 bits (535), Expect = 1.2e-54
Identity = 120/180 (66.67%), Postives = 136/180 (75.56%), Query Frame = 0


Query: 43  LHSSYSSSSSSNRCNFIPLRSVLKTVESSELEVKN--------SDLVDPTFPNDRSTILV 102
           LH  Y++S          + + LKTVE + L   N        SD  +PT P  R  ILV
Sbjct: 35  LHRRYATS-----VKLTAISAALKTVEQTTLTEDNRFSTVGSDSDEYNPTLPKPR--ILV 94

Query: 103 AEKLGEAGLKLLRSFGNVECAYDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLK 162
            EKLGEAG+ LLR FG+V+C+YDLSPEDL  K++  DALIVRSGTKV R+VFEAAKGRLK
Sbjct: 95  TEKLGEAGVNLLREFGDVDCSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEAAKGRLK 154

Query: 163 VVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAG 215
           VVGRAGVGIDNVDLQAATE GCLVVNAPTANT+AAAEHGIALL +MARNVAQADAS+KAG
Sbjct: 155 VVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASMARNVAQADASIKAG 207

[Close]

BLAST of Lsi07G006290 vs. TAIR 10
Match: AT3G19480.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 197.2 bits (500), Expect = 1.3e-50
Identity = 114/185 (61.62%), Postives = 136/185 (73.51%), Query Frame = 0


Query: 35  SVSLNLHPLHSSYSSSSSSNRCNFIPLRS----VLKTVESSELEVKNSDLVDPTFPNDRS 94
           + SLNL  + SS SS   +   +  P+R     +L T  SS                 + 
Sbjct: 2   ATSLNLSSIFSS-SSRLVTTPSSVFPIRQRRRIILVTSSSSG-------------GGGKP 61

Query: 95  TILVAEKLGEAGLKLLRSFGNVECAYDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAK 154
           TILV EKLG+AG+ LL+ + NV+C+YDLS E+LC KIS CDALIVRSGTKV R VFE+++
Sbjct: 62  TILVTEKLGQAGIDLLKKYANVDCSYDLSLEELCTKISLCDALIVRSGTKVGRDVFESSR 121

Query: 155 GRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADAS 214
           GRLKVVGRAGVGIDNVDL AATE+GCLVVNAPTANT+AAAEHGIALLTAMARN+AQADAS
Sbjct: 122 GRLKVVGRAGVGIDNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADAS 172

Query: 215 MKAGR 216
           +KAG+
Sbjct: 182 IKAGK 172

[Close]

BLAST of Lsi07G006290 vs. TAIR 10
Match: AT4G34200.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 193.7 bits (491), Expect = 1.5e-49
Identity = 109/173 (63.01%), Postives = 129/173 (74.57%), Query Frame = 0


Query: 42  PLHSSYSSSSSSNRCNFIPLRSVLKTVESSELEVKNSDLVDPTFPNDRSTILVAEKLGEA 101
           PL S+ S +  S   N +  R VL +  + +                + TILVAEKLG+A
Sbjct: 28  PLPSAISVAFPSRGRNTLQRRLVLVSCSTGD--------------GSKPTILVAEKLGDA 87

Query: 102 GLKLLRSFGNVECAYDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGV 161
           G+KLL    NV+C+Y+++PE+L  KIS CDALIVRSGTKV R+VFE++ GRLKVVGRAGV
Sbjct: 88  GIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGREVFESSHGRLKVVGRAGV 147

Query: 162 GIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAG 215
           GIDNVDL AATEFGCLVVNAPTANTIAAAEHGIAL+ AMARNVAQADAS+KAG
Sbjct: 148 GIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADASVKAG 186

[Close]

BLAST of Lsi07G006290 vs. TAIR 10
Match: AT1G68010.1 (hydroxypyruvate reductase )

HSP 1 Score: 45.1 bits (105), Expect = 8.5e-05
Identity = 34/99 (34.34%), Postives = 48/99 (48.48%), Query Frame = 0


Query: 118 LSPEDLCAKI-SSCDALIVRSGTKVNRQVFEA-AKGRLKVVGRAGVGIDNVDLQAATEFG 177
           LS ED+   I   CD +I +        +F A +K   K      VG +NVD++AA ++G
Sbjct: 50  LSVEDIIDLIGDKCDGVIGQLTEDWGETLFSALSKAGGKAFSNMAVGYNNVDVEAANKYG 109

Query: 178 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAG 215
             V N P   T   AE   +L  A AR + +AD  M+ G
Sbjct: 110 IAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRGG 148

The following BLAST results are available for this feature:

BLAST of Lsi07G006290 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04130	9.7e-54	66.30	D-3-phosphoglycerate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
Q9LT69	1.9e-49	61.62	D-3-phosphoglycerate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
O49485	2.1e-48	63.01	D-3-phosphoglycerate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
Q58424	1.7e-26	53.66	D-3-phosphoglycerate dehydrogenase OS=Methanocaldococcus jannaschii (strain ATCC...	[more]
O29445	3.6e-24	48.80	D-3-phosphoglycerate dehydrogenase OS=Archaeoglobus fulgidus (strain ATCC 49558 ...	[more]

BLAST of Lsi07G006290 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VDA4	5.3e-95	91.20	D-3-phosphoglycerate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A1S3CKG7	5.3e-95	91.20	D-3-phosphoglycerate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103501977 PE=3 ...	[more]
A0A5D3CA37	2.6e-94	89.09	D-3-phosphoglycerate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A0A0LC35	8.4e-93	89.35	D-3-phosphoglycerate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_3G651740 PE...	[more]
A0A6J1IWT4	2.5e-84	83.64	D-3-phosphoglycerate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111479899 P...	[more]

BLAST of Lsi07G006290 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895985.1	7.0e-102	96.76	D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Benincasa hispida]	[more]
KAA0065618.1	1.1e-94	91.20	D-3-phosphoglycerate dehydrogenase 1 [Cucumis melo var. makuwa]	[more]
XP_008463985.1	1.1e-94	91.20	PREDICTED: D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucumis mel...	[more]
TYK07186.1	5.4e-94	89.09	D-3-phosphoglycerate dehydrogenase 1 [Cucumis melo var. makuwa]	[more]
XP_022980570.1	5.1e-84	83.64	D-3-phosphoglycerate dehydrogenase 2, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of Lsi07G006290 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17745.1	6.9e-55	66.30	D-3-phosphoglycerate dehydrogenase	[more]
AT1G17745.2	1.2e-54	66.67	D-3-phosphoglycerate dehydrogenase	[more]
AT3G19480.1	1.3e-50	61.62	D-3-phosphoglycerate dehydrogenase	[more]
AT4G34200.1	1.5e-49	63.01	D-3-phosphoglycerate dehydrogenase	[more]
AT1G68010.1	8.5e-05	34.34	hydroxypyruvate reductase	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 89..213 e-value: 9.6E-35 score: 121.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR42938	FORMATE DEHYDROGENASE 1	coord: 48..215
None	No IPR available	PANTHER	PTHR42938:SF22	D-3-PHOSPHOGLYCERATE DEHYDROGENASE 2, CHLOROPLASTIC	coord: 48..215
None	No IPR available	SUPERFAMILY	52283	Formate/glycerate dehydrogenase catalytic domain-like	coord: 91..214
IPR006139	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain	PFAM	PF00389	2-Hacid_dh	coord: 92..205 e-value: 4.7E-29 score: 100.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi07G006290.1	Lsi07G006290.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006564	L-serine biosynthetic process
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0004617	phosphoglycerate dehydrogenase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor