Lsi07G006100 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G006100
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionprotein BIG GRAIN 1-like B
Locationchr07: 6566422 .. 6567537 (+)
RNA-Seq ExpressionLsi07G006100
SyntenyLsi07G006100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTGTGGGATCACTCCTTTCACAGCAGAGATTCAGACCACCTCCAACAACGCCGTCGTTCTCGCCGTGAAAACCCTTCTTTCTCCTCCTCTCTTCTTGACGCTATCTACCGTTCCATCGACGAATCCAACGCCCAGCCTGAAGACCACCTCATTTTCTACACTCAAAAAACTACTGTCAGAACAAAACAGAGTATCGCCGCCGCCAGAACAGAGGACGACGAAACTCTCAAATTCCGACGAGCTTGCATGATCGATAAGTGGATGGATAAGAAAAAACTCAGAAACATTCGTGACTTTACTCTTTCCAGCTCCAGCTCGTCCGAATCCAGCTCCACTGCTGGCAGAAGATTTTCCTCGTCGGAATCGGAGTTTCTGTCACGTGCACTGCACAGGCCGAAGCCGAAGCCGAAGCCGATCAAAACCACTACATGGGCTCAAACAGAAATTATTAGTCCAAATCCAAAGCATGAAAATGGGTTCGTTAAATCCAAATCCAAAGCTTCAAAAATCTATCACGATTTGAAGAAAGTGAAACAGCCGATTTCACCCGGTGGTCGTCTTGCCAGCTTTCTCAATTCCCTCTTCAATGGCGGAAGCCCAAAAACGAAGCAAAAGATATCAAACGCCTCTTCAATCAATTCAACGAATTTCGATTACGACATGTCGAGAAAATCCAAATCTCAACAGGGCTCGACATCTACCTGTTCGTCTGCTTCTTCGTTTTCAAGGTCTTGTTTGAGCAAAACGCCATCTTCAAGAGGGAACATTAAGAGATCGGTTCGGTTTTGTCCAGTGAGTGTTATAGTGGACGAGGATTGTCAGCCATGTGGGCATAAAATTTTGCACAAATTAGAGGAACCCATTATGAAGAAAGGTAATTTGAAAAGTTATGGGAATTTCGAAACAGAGAAAGTGAAAACAGAGCATGAGATGAAGATTAGTTATGAGGATGAAGATGATGATGATGCTTTGAGTTGTTCCAGTTCTGATCTGTTTGAATTGGATAATCTTTCAGTTATTGGAATTGAAAGATACAGAGAAGAATTGCCAGTGTATGAAACTACCCATTTCAAAACTAATTGTGCCATTGCTAATGGATTGGTTTTGTAA

mRNA sequence

ATGGATTTGTGGGATCACTCCTTTCACAGCAGAGATTCAGACCACCTCCAACAACGCCGTCGTTCTCGCCGTGAAAACCCTTCTTTCTCCTCCTCTCTTCTTGACGCTATCTACCGTTCCATCGACGAATCCAACGCCCAGCCTGAAGACCACCTCATTTTCTACACTCAAAAAACTACTGTCAGAACAAAACAGAGTATCGCCGCCGCCAGAACAGAGGACGACGAAACTCTCAAATTCCGACGAGCTTGCATGATCGATAAGTGGATGGATAAGAAAAAACTCAGAAACATTCGTGACTTTACTCTTTCCAGCTCCAGCTCGTCCGAATCCAGCTCCACTGCTGGCAGAAGATTTTCCTCGTCGGAATCGGAGTTTCTGTCACGTGCACTGCACAGGCCGAAGCCGAAGCCGAAGCCGATCAAAACCACTACATGGGCTCAAACAGAAATTATTAGTCCAAATCCAAAGCATGAAAATGGGTTCGTTAAATCCAAATCCAAAGCTTCAAAAATCTATCACGATTTGAAGAAAGTGAAACAGCCGATTTCACCCGGTGGTCGTCTTGCCAGCTTTCTCAATTCCCTCTTCAATGGCGGAAGCCCAAAAACGAAGCAAAAGATATCAAACGCCTCTTCAATCAATTCAACGAATTTCGATTACGACATGTCGAGAAAATCCAAATCTCAACAGGGCTCGACATCTACCTGTTCGTCTGCTTCTTCGTTTTCAAGGTCTTGTTTGAGCAAAACGCCATCTTCAAGAGGGAACATTAAGAGATCGGTTCGGTTTTGTCCAGTGAGTGTTATAGTGGACGAGGATTGTCAGCCATGTGGGCATAAAATTTTGCACAAATTAGAGGAACCCATTATGAAGAAAGGTAATTTGAAAAGTTATGGGAATTTCGAAACAGAGAAAGTGAAAACAGAGCATGAGATGAAGATTAGTTATGAGGATGAAGATGATGATGATGCTTTGAGTTGTTCCAGTTCTGATCTGTTTGAATTGGATAATCTTTCAGTTATTGGAATTGAAAGATACAGAGAAGAATTGCCAGTGTATGAAACTACCCATTTCAAAACTAATTGTGCCATTGCTAATGGATTGGTTTTGTAA

Coding sequence (CDS)

ATGGATTTGTGGGATCACTCCTTTCACAGCAGAGATTCAGACCACCTCCAACAACGCCGTCGTTCTCGCCGTGAAAACCCTTCTTTCTCCTCCTCTCTTCTTGACGCTATCTACCGTTCCATCGACGAATCCAACGCCCAGCCTGAAGACCACCTCATTTTCTACACTCAAAAAACTACTGTCAGAACAAAACAGAGTATCGCCGCCGCCAGAACAGAGGACGACGAAACTCTCAAATTCCGACGAGCTTGCATGATCGATAAGTGGATGGATAAGAAAAAACTCAGAAACATTCGTGACTTTACTCTTTCCAGCTCCAGCTCGTCCGAATCCAGCTCCACTGCTGGCAGAAGATTTTCCTCGTCGGAATCGGAGTTTCTGTCACGTGCACTGCACAGGCCGAAGCCGAAGCCGAAGCCGATCAAAACCACTACATGGGCTCAAACAGAAATTATTAGTCCAAATCCAAAGCATGAAAATGGGTTCGTTAAATCCAAATCCAAAGCTTCAAAAATCTATCACGATTTGAAGAAAGTGAAACAGCCGATTTCACCCGGTGGTCGTCTTGCCAGCTTTCTCAATTCCCTCTTCAATGGCGGAAGCCCAAAAACGAAGCAAAAGATATCAAACGCCTCTTCAATCAATTCAACGAATTTCGATTACGACATGTCGAGAAAATCCAAATCTCAACAGGGCTCGACATCTACCTGTTCGTCTGCTTCTTCGTTTTCAAGGTCTTGTTTGAGCAAAACGCCATCTTCAAGAGGGAACATTAAGAGATCGGTTCGGTTTTGTCCAGTGAGTGTTATAGTGGACGAGGATTGTCAGCCATGTGGGCATAAAATTTTGCACAAATTAGAGGAACCCATTATGAAGAAAGGTAATTTGAAAAGTTATGGGAATTTCGAAACAGAGAAAGTGAAAACAGAGCATGAGATGAAGATTAGTTATGAGGATGAAGATGATGATGATGCTTTGAGTTGTTCCAGTTCTGATCTGTTTGAATTGGATAATCTTTCAGTTATTGGAATTGAAAGATACAGAGAAGAATTGCCAGTGTATGAAACTACCCATTTCAAAACTAATTGTGCCATTGCTAATGGATTGGTTTTGTAA

Protein sequence

MDLWDHSFHSRDSDHLQQRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTTVRTKQSIAAARTEDDETLKFRRACMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGRRFSSSESEFLSRALHRPKPKPKPIKTTTWAQTEIISPNPKHENGFVKSKSKASKIYHDLKKVKQPISPGGRLASFLNSLFNGGSPKTKQKISNASSINSTNFDYDMSRKSKSQQGSTSTCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKLEEPIMKKGNLKSYGNFETEKVKTEHEMKISYEDEDDDDALSCSSSDLFELDNLSVIGIERYREELPVYETTHFKTNCAIANGLVL
Homology
BLAST of Lsi07G006100 vs. ExPASy Swiss-Prot
Match: Q9SLL2 (Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.1e-57
Identity = 175/397 (44.08%), Postives = 217/397 (54.66%), Query Frame = 0

Query: 1   MDLWDHSFHSRDSDHLQQRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTT 60
           MD WD      D  H  + RR  R +PSFSS+LLD IYRSID+S+           +KT 
Sbjct: 1   MDPWDTYNIPLDHQHRLRNRRDHR-HPSFSSTLLDQIYRSIDDSSTNSSS-----MRKTK 60

Query: 61  VRTKQSIAAARTEDDETLKFRRACMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGRRFS 120
            + ++    +    D+   F R+         K L+ I       SSSS S S+    FS
Sbjct: 61  HQNREDTRVSANRRDD---FNRS---------KNLKTIEPVFFKHSSSSSSDSSG---FS 120

Query: 121 SSESE-FLSRALHRPK-PKPKPIKTTT-----WAQTEIISPNPKHENG-FVKSKSKASKI 180
           SSES+ F  R+   P    PKPI+TT        Q    + + K E+G F+K+KSKA KI
Sbjct: 121 SSESDYFYRRSKSSPAISHPKPIRTTVERFERSPQNHRPNSSNKQEHGSFLKTKSKALKI 180

Query: 181 YHDLKKVKQPISPGGRLASFLNSLFNGGSPKTKQKISNASSINSTNFDYDMSRKSKSQQG 240
           Y DLKKVKQPISPGGRLA+FLNS+F G          N   +N  N     +  + +   
Sbjct: 181 YSDLKKVKQPISPGGRLATFLNSIFTGA--------GNTKKLNKINTTVTSTTAAAAAAS 240

Query: 241 STSTCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHK----------- 300
           ST+TCSSASSFSRSCLSKTPSS    KRSVRFCPV+VI DED     +K           
Sbjct: 241 STTTCSSASSFSRSCLSKTPSSSEKSKRSVRFCPVNVIFDEDSSKYNNKNNKVYGNNERE 300

Query: 301 ---ILHKLEEPIMKKGN---------LKSYGNFETEKVKTEHEMKISYEDEDDDDALSCS 360
              I H LE  +M++           L+SY     E ++   E     E++DDDDALSC+
Sbjct: 301 YESIRHTLENRVMEENRRVIEAAKELLRSYQKKNKEVIEVSVE---DDEEDDDDDALSCT 360

Query: 361 SSDLFELDNLSVIGIERYREELPVYETTHFKTNCAIA 367
           SSDLFELDNLS IGI+RYREELPVYETT   TN  I+
Sbjct: 361 SSDLFELDNLSAIGIDRYREELPVYETTRLNTNRIIS 365

BLAST of Lsi07G006100 vs. ExPASy Swiss-Prot
Match: Q9LVK2 (Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 8.9e-55
Identity = 169/375 (45.07%), Postives = 211/375 (56.27%), Query Frame = 0

Query: 22  SRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTTVRTKQSIAAARTEDDE--TLK 81
           S   NPSFSS+LLD IYRSID+S+  P    I    K      Q   A+  ED E   + 
Sbjct: 12  SHHRNPSFSSTLLDQIYRSIDDSSPPPPLESI----KKKKHHHQQRNASLHEDREISPIY 71

Query: 82  FRRACMIDKWMDKKKLRNIRDFTLSSS-----SSSESSSTAGRRFSSSESEFLSRALHRP 141
            RR+   D    ++K   +R    SSS     SSSES S  GR  SS+     SR     
Sbjct: 72  HRRSIAADFERSRRKTDFLRHSNSSSSDSSGFSSSESDSFHGRSKSSASPPSSSR----- 131

Query: 142 KPKPKPIKTTTWAQTEIISPNPKHENGFVKSKSKASKIYHDLKKVKQPISPGGRLASFLN 201
             +PKPI+T++   +  +   PK   GF+++KSKA KIY DLKKVKQPISPGGRLA+FLN
Sbjct: 132 -QQPKPIRTSSVDHSSAVQ-KPKELGGFLRTKSKALKIYSDLKKVKQPISPGGRLATFLN 191

Query: 202 SLFNGG--SPKTKQKISNASSINSTNFDYDMSRKSKSQQGSTSTCSSASSFSRSCLSKTP 261
           SLF     +PK  +K +  + +   +              S+STCSSASSFSRSCLSKTP
Sbjct: 192 SLFTNAATNPKKHKKTTTVAVVEEPH--------------SSSTCSSASSFSRSCLSKTP 251

Query: 262 SSRGNIKRSVRFCPVSVIVDED---CQPCGHKILHKLEEPIMK------------KGNLK 321
           SS G  KRSVRFCPV+VI+DED     P  +      +    K            K  L+
Sbjct: 252 SSSGKSKRSVRFCPVNVILDEDSSFTMPYAYNNERLYDNNEAKRVEEHRRVIQAAKDLLR 311

Query: 322 SYGNFETEKVKTEHEMKISYEDEDDDDALSCSSSDLFELDNLSVIGIERYREELPVYETT 372
           +Y N    KV T +   +  E++D+DDA SC+SSDLFEL+NLS IGIERYREELPVYETT
Sbjct: 312 TYHN--KNKVTTTNINNV--EEDDEDDAASCASSDLFELENLSAIGIERYREELPVYETT 357

BLAST of Lsi07G006100 vs. ExPASy Swiss-Prot
Match: Q9M2B3 (Protein BIG GRAIN 1-like C OS=Arabidopsis thaliana OX=3702 GN=At3g42800 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.6e-30
Identity = 133/374 (35.56%), Postives = 185/374 (49.47%), Query Frame = 0

Query: 23  RRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTTVRTKQSIAAARTEDDETLKFRR 82
           R+  PSFSSS+LD++YRSIDES+    D  +  +    V +  S  +   +DD+    RR
Sbjct: 6   RKRTPSFSSSVLDSVYRSIDESDGLQSD--LKGSINENVSSSSSSPSPNKKDDKLTTLRR 65

Query: 83  ACMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGRRFSSSESEFLSRALHRPKPKPKPIK 142
           A      MD++     R  T +++SS  SS      FSSSE+E         K   +  +
Sbjct: 66  AI-----MDEEHWLYARSSTTTTNSSDSSS------FSSSEAESYRTKRRLRKLAEQGKR 125

Query: 143 TTTWAQTEIISPNPKHENGFVKS----KSKASKIYHDLKKVKQPISPGGRLASFLNSLFN 202
           +    Q    +        F KS    K KA KI  +LK+ KQP+SPG RL SFLNS+F 
Sbjct: 126 SGDERQRTKRTVMDNDSRLFSKSDDDKKPKAVKIIEELKRSKQPVSPGARLTSFLNSIFQ 185

Query: 203 GGSPKTKQKISNASSINSTNFDYDMSRKSKSQQGSTSTCSSASSFSRSCLSKTPSSRGNI 262
             + K K       S+  T                  + SS S FSR+  +KT ++  N 
Sbjct: 186 SNAKKVK-----LCSVGKTT--------------DVKSSSSKSCFSRT-RNKTDNNNNNC 245

Query: 263 K---RSVRFCPVSVIVDEDCQPCGHKILHKLEEPIMKKGNLKSYGNFETEKVKT-EHEMK 322
           K   RS+RF PV V +D DC+    K + ++ +PI +    KS      E++KT +H  +
Sbjct: 246 KKLERSIRFYPVRVTIDGDCRDYAQKHITRVRKPIPEFTAKKSV----KEEIKTNDHHTE 305

Query: 323 IS----------------YE-DEDDDDALSCSSSDLFELDNLSVIGIERYREELPVYETT 372
            +                YE  E+++DA S SSSDLFELD+   IG+ RY +ELPVYETT
Sbjct: 306 FTCITRNIGLKDFVRSNKYEGKEEEEDAWSHSSSDLFELDSYR-IGMGRYLKELPVYETT 341

BLAST of Lsi07G006100 vs. ExPASy Swiss-Prot
Match: Q9LYH0 (Protein BIG GRAIN 1-like D OS=Arabidopsis thaliana OX=3702 GN=At5g12050 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.1e-25
Identity = 138/385 (35.84%), Postives = 179/385 (46.49%), Query Frame = 0

Query: 24  RENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTTVRTKQSIAAARTEDDETLKFRRA 83
           + NPSFSS+LLD IY SID    + + ++       T   KQSI      D         
Sbjct: 5   KRNPSFSSTLLDEIYNSIDPKTQKTQPYV---GSVNTTTKKQSIVTRSVPD--------- 64

Query: 84  CMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGRRFSSSESEFL-------SRALHRPKP 143
                    +K+   R F   SSSS  +SS     FSSS++E         SR L     
Sbjct: 65  ---------RKIHRDRFFGSVSSSSDSNSSI----FSSSDTELTHGKKTTSSRPLCFGPS 124

Query: 144 KPKPIK------------TTTWAQTEIISPNPK---HENGFVKSKSKAS-KIYHDLKKVK 203
           K KP K            T  +   +  S  PK   H+  +  ++++ S K   + KK K
Sbjct: 125 KTKPRKTEDKTLFHQNRATRVYDDYDYASDVPKFNRHDENWENTRNRRSVKSSGNQKKPK 184

Query: 204 QPISPGGRLASFLNSLFNGGSPKTKQKISNASSINSTNFDYDMS----RKSKSQQGSTST 263
            P SPGGR+ +FLNSLF+  S     K SNA         YD S    + S     ST+T
Sbjct: 185 TPASPGGRIVNFLNSLFSNNS-----KQSNAVKSYPRKTSYDDSAYVRKTSNDYHSSTTT 244

Query: 264 CSSASSFSRSCLSK-TPSSRGNIKRSVRFCPVSVIV-------DEDCQPCGH---KILHK 323
           CSSASSFSRSC++K    S G IKRSVRF PV+VIV       +ED    G+    +   
Sbjct: 245 CSSASSFSRSCMNKGYEKSSGRIKRSVRFSPVNVIVPESFTSKEEDYFSNGNARKSVKKN 304

Query: 324 LE-------EPIMKKGNLKSYGNFETEKVKTE--HEMKISYEDEDDDDALSCSSSDLFEL 358
           +E       E I ++     + N E   VKT    + +   ED+DDDD  S SSSDLFEL
Sbjct: 305 VEDGGRRSVEEIAREFLRDYHKNHENSLVKTNGFEDYEDDDEDDDDDDVASDSSSDLFEL 356

BLAST of Lsi07G006100 vs. ExPASy Swiss-Prot
Match: Q10R09 (Protein BIG GRAIN 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BG1 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.8e-24
Identity = 120/355 (33.80%), Postives = 174/355 (49.01%), Query Frame = 0

Query: 20  RRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTTVRTKQSIAAARTEDDETL- 79
           RR   + PSFSS+LLDAIY+S+DE                    +   AAA+ + +  L 
Sbjct: 15  RRYVADQPSFSSTLLDAIYKSMDEQPGHGGG---------ATGVEAVAAAAKKQHEAALH 74

Query: 80  --KFRRACMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGRRFSSSESEFLSRALHRPKP 139
              + +  +   +  +    +      ++SSSSE SS  G  FSSSE+E    + HR   
Sbjct: 75  YGNYYKPSLAGSYRARAPGPH-----ATTSSSSECSSYGG--FSSSEAE---SSHHR--- 134

Query: 140 KPKPIKTTTWAQTEIISPNPKHENGFVKSKSKASKIYHDLKKVKQPISPGGRLASFLNSL 199
           + +PI+TT        +P P  E    K+K   + I   L+ +++P SPG RLA FLNS+
Sbjct: 135 RLRPIRTTVPGG----APGPAPEK---KAKKPGASIRAKLRDLRKPASPGARLAGFLNSI 194

Query: 200 FNGGSPKTKQKISNASSINSTNFDYDMSRKSKSQQGSTSTCSSASSFSRSCLSKTPSSRG 259
           F G       K + A+  ++T              G+ S CS+ASS+SRSCLSKTPS+RG
Sbjct: 195 FAG-------KRAPATPPSAT-------------AGAESACSTASSYSRSCLSKTPSTRG 254

Query: 260 NIKRSVRFCPVSVIVDEDCQP-CGHKILHKLEEPIMKKGNLKSYGNFETEKVKTEHEMKI 319
             KR+VRF      +D D +      ++ +   P+              E V+     ++
Sbjct: 255 QAKRTVRF------LDSDTESLASSTVVDRRRVPV--------------EAVQQMLLQRM 300

Query: 320 SYEDEDDDDALSCSSSDLFELDNLSVIGI--ERYREELPVYETTHFKTNCAIANG 369
             E ++DDD  S +SSDLFEL+N + I      YR+ELPVYETT    N AI +G
Sbjct: 315 EMESDEDDDESSDASSDLFELENFAAIAPAGAAYRDELPVYETTRVALNRAIGHG 300

BLAST of Lsi07G006100 vs. ExPASy TrEMBL
Match: A0A1S3CKK1 (protein BIG GRAIN 1-like B OS=Cucumis melo OX=3656 GN=LOC103501995 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 4.7e-160
Identity = 321/385 (83.38%), Postives = 336/385 (87.27%), Query Frame = 0

Query: 1   MDLWDHSFHSRDSDHLQQRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTT 60
           MDLWDHS    D   LQQ RRSRR+NPSFSSSLLDAIYRSIDESN+QPEDHLIFYT  TT
Sbjct: 1   MDLWDHS----DPHLLQQPRRSRRDNPSFSSSLLDAIYRSIDESNSQPEDHLIFYTHNTT 60

Query: 61  VRTKQSIAAARTEDDETLKFRRACMIDKWMD-KKKLRNIRDFTLSSSSSSESSSTAGRRF 120
             T   + A  ++D E+LKFR   MID W+D KKKLRNIRDFTLSSSSSSESSSTAGRRF
Sbjct: 61  KHT--ILPATVSQDHESLKFR---MIDTWIDKKKKLRNIRDFTLSSSSSSESSSTAGRRF 120

Query: 121 SSSESEFLSRALHRP---KPKPKPIKTTTWAQTEIISPNPKHENGFVKSKSKASKIYHDL 180
           SSSE+EFLSRALHRP   KPKPKPIKT TW QTEII+PNPKH+NGFVKSKSKASKIYHDL
Sbjct: 121 SSSETEFLSRALHRPTKLKPKPKPIKTNTWPQTEIITPNPKHDNGFVKSKSKASKIYHDL 180

Query: 181 KKVKQPISPGGRLASFLNSLFNGGSPKTKQKISNAS-SINSTNFDYDMSRKSKSQQGSTS 240
           KKVKQPISPG RLASFLNSLFNGGSPK KQKIS+++ SINST FDYDMSRKSKSQQGSTS
Sbjct: 181 KKVKQPISPGARLASFLNSLFNGGSPKAKQKISSSTCSINSTKFDYDMSRKSKSQQGSTS 240

Query: 241 TCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKLEEPIMKKGN 300
           TCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHK EEPIMKKGN
Sbjct: 241 TCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKSEEPIMKKGN 300

Query: 301 L-KSYGNFETEKVKTEHEMKISYE--------DEDDDDALSCSSSDLFELDNLSVIGIER 360
           L KS GN E EKVKTE  MKI+YE        D+DDDDALSCSSSDLFELDNLSVIGIER
Sbjct: 301 LKKSNGNLEREKVKTEDMMKINYEEEEEEDDDDDDDDDALSCSSSDLFELDNLSVIGIER 360

Query: 361 YREELPVYETTHFKTNCAIANGLVL 372
           YREELPVYETTHFKTNCAIA GLVL
Sbjct: 361 YREELPVYETTHFKTNCAIAKGLVL 376

BLAST of Lsi07G006100 vs. ExPASy TrEMBL
Match: A0A6J1IZW5 (protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111479960 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 8.6e-154
Identity = 299/377 (79.31%), Postives = 324/377 (85.94%), Query Frame = 0

Query: 1   MDLWDHSFHSRDSD--HLQQRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQK 60
           MDLWD+SF   D D  H  QRRRSRRENPSFSSSLLDAIYRSID+SN Q ED LIFYTQK
Sbjct: 1   MDLWDNSFQREDPDPHHHHQRRRSRRENPSFSSSLLDAIYRSIDDSNVQGEDQLIFYTQK 60

Query: 61  TTVRTKQSIAAARTEDDETLKFRRACMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGRR 120
           TT RTKQSI+AA+TED +TL+F+RA MI  WMD KK++ +RDFT+S+SSSSESSSTAGRR
Sbjct: 61  TTTRTKQSISAAKTEDHQTLQFQRASMIKNWMDNKKIKTVRDFTVSTSSSSESSSTAGRR 120

Query: 121 FSSSESEFLSRALHR----PKPKPKPIKTTTWAQTEIISPNPKHENGFVKSKSKASKIYH 180
            SSSESEFLSR LHR    PKPKPKPIKTTTW+QTEII PNP HENGFVKSK +ASKIYH
Sbjct: 121 LSSSESEFLSRTLHRPKPKPKPKPKPIKTTTWSQTEII-PNPNHENGFVKSKFRASKIYH 180

Query: 181 DLKKVKQPISPGGRLASFLNSLFNGGSPKTKQKISNASSINSTNFDYDMSRKSKSQQGST 240
           DLKKVKQPISPGGRLA+FLNSLFNGGSPK KQKISN  S NSTN DYD  RKSKSQQGS+
Sbjct: 181 DLKKVKQPISPGGRLANFLNSLFNGGSPKAKQKISNTCSNNSTNLDYDAPRKSKSQQGSS 240

Query: 241 STCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKLEEPIMKKG 300
           STCSSASSFSRSCL KTPSSRGNIKRSVRFCPVSVIVDEDC+PCGHK+LHKLEEPI KKG
Sbjct: 241 STCSSASSFSRSCLIKTPSSRGNIKRSVRFCPVSVIVDEDCRPCGHKMLHKLEEPIGKKG 300

Query: 301 NLKSYGNFETEKVKTEHEMKISYEDEDDDDALSCSSSDLFELDNLSVIGIERYREELPVY 360
            L      ETE++KTE       ED++DDD LSCSSSDLFELDNLS IG+ERYR+ELPVY
Sbjct: 301 KL------ETEEMKTE-------EDDEDDDRLSCSSSDLFELDNLSAIGMERYRDELPVY 360

Query: 361 ETTHFKTNCAIANGLVL 372
           ETTHF TNCAIANGL+L
Sbjct: 361 ETTHFNTNCAIANGLLL 363

BLAST of Lsi07G006100 vs. ExPASy TrEMBL
Match: A0A6J1GWE3 (protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111457754 PE=3 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 3.1e-151
Identity = 295/377 (78.25%), Postives = 323/377 (85.68%), Query Frame = 0

Query: 1   MDLWDHSFHSRDSD----HLQQRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYT 60
           MDLWD+SF   D D    H  QRRRSRRENPSFSSSLLDAIYRSID+SNAQ ED LIFYT
Sbjct: 1   MDLWDNSFQREDPDPHHHHHHQRRRSRRENPSFSSSLLDAIYRSIDDSNAQGEDQLIFYT 60

Query: 61  QKTTVRTKQSIAAARTEDDETLKFRRACMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAG 120
           QKTT RTKQSI+AA+TED +TL+FRRA MI+ WMD KK++ +RDFT+S+SSSSESSSTAG
Sbjct: 61  QKTTTRTKQSISAAKTEDHQTLQFRRASMIENWMDNKKIKTLRDFTVSTSSSSESSSTAG 120

Query: 121 RRFSSSESEFLSRALHR--PKPKPKPIKTTTWAQTEIISPNPKHENGFVKSKSKASKIYH 180
           RR SSSESEFLSR LHR  PKPKPKPIKTTTW+QTEII PNP HENGFVKSK KASKIYH
Sbjct: 121 RRLSSSESEFLSRTLHRPKPKPKPKPIKTTTWSQTEII-PNPNHENGFVKSKFKASKIYH 180

Query: 181 DLKKVKQPISPGGRLASFLNSLFNGGSPKTKQKISNASSINSTNFDYDMSRKSKSQQGST 240
           DLKKVKQPISPGGRLASFLNSLFNGGSPK KQKISN  S NSTN +Y+  RKSKSQQGS+
Sbjct: 181 DLKKVKQPISPGGRLASFLNSLFNGGSPKAKQKISNTCSNNSTNLEYEAPRKSKSQQGSS 240

Query: 241 STCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKLEEPIMKKG 300
           STCSSASSFSRSCL KTPSSRGNIKRSVRFCPVSVIVDEDC+PCGHK+L+KLEEPI KKG
Sbjct: 241 STCSSASSFSRSCLIKTPSSRGNIKRSVRFCPVSVIVDEDCRPCGHKMLNKLEEPIGKKG 300

Query: 301 NLKSYGNFETEKVKTEHEMKISYEDEDDDDALSCSSSDLFELDNLSVIGIERYREELPVY 360
                      K++TE +     +++DDDD LSCSSSDLFELDNLS IG+ERYR+ELPVY
Sbjct: 301 -----------KLETEED-----DEDDDDDRLSCSSSDLFELDNLSAIGMERYRDELPVY 360

Query: 361 ETTHFKTNCAIANGLVL 372
           ETTHF TNCAIANGL+L
Sbjct: 361 ETTHFNTNCAIANGLLL 360

BLAST of Lsi07G006100 vs. ExPASy TrEMBL
Match: A0A6J1KB46 (protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111493335 PE=3 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 4.8e-144
Identity = 288/375 (76.80%), Postives = 320/375 (85.33%), Query Frame = 0

Query: 1   MDLWDHSFHSRDSDHLQQRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTT 60
           MDLWD SF   D D L QRRRSRR+NPSFSSSLLDAIYRSIDESN Q ED  +F+ ++TT
Sbjct: 84  MDLWDKSFQRHDQD-LPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETT 143

Query: 61  VRTKQSIAAARTEDDETLKFRRACMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGRRFS 120
           +RTKQS+  ARTEDDE +KFRRACMI+KWMD+KK+RNIRDFT+SSSSSSES+STAGRR+S
Sbjct: 144 MRTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYS 203

Query: 121 SSESEFLSRALHRPKPKPKPIKTTTWAQTEIISPNPKHENGFVKSKSKASKIYHDLKKVK 180
           +SESE LSRALHR     KPIKTT W QTE  +   KHENG VK+KSKASKIYHDLKKVK
Sbjct: 204 ASESELLSRALHR----QKPIKTTPWTQTE--TQKAKHENGLVKTKSKASKIYHDLKKVK 263

Query: 181 QPISPGGRLASFLNSLFNGGSPKTKQKISNASSINSTNFDYDMSRKSKSQQGSTSTCSSA 240
           QPISPGGRLA+FLNSLFNGGSPKTKQK+ N S+ N T+ DYDM  K+KSQ    STCSSA
Sbjct: 264 QPISPGGRLANFLNSLFNGGSPKTKQKMPNPST-NPTSMDYDMPTKAKSQ---GSTCSSA 323

Query: 241 SSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKLEEPIMKKGNLKSYG 300
           SSFSRSCLSKTPSSRGN+KRSVRFCPVS IVDEDC+  GHK L KLEEPIMKKG LKSYG
Sbjct: 324 SSFSRSCLSKTPSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKG-LKSYG 383

Query: 301 NFETEKVKTEHEMKI----SYEDEDDDDALSCSSSDLFELDNLSVIGIERYREELPVYET 360
           NFETE+VKTEH  KI     +E ED+DDA+SCSSSDLFELDNLSVIGIER+REELPVYET
Sbjct: 384 NFETEEVKTEHPRKIKRNEGFEYEDEDDAVSCSSSDLFELDNLSVIGIERFREELPVYET 443

Query: 361 THFKTNCAIANGLVL 372
           T+FKTNCAIA GL++
Sbjct: 444 TNFKTNCAIAKGLLV 446

BLAST of Lsi07G006100 vs. ExPASy TrEMBL
Match: A0A6J1GAP7 (protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111452438 PE=3 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 3.4e-142
Identity = 288/381 (75.59%), Postives = 318/381 (83.46%), Query Frame = 0

Query: 1   MDLWDHSFHSRDSDHLQQRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTT 60
           MDLWD SF   D D L QRRRSRR+NPSFSSSLLDAIYRSIDESN Q ED  +F+ ++TT
Sbjct: 1   MDLWDKSFQRHDQD-LPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETT 60

Query: 61  VRTKQSIAAARTEDDETLKFRRACMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGRRFS 120
           +RTKQS+  ARTEDDE +KFRRACMI+KWMD+KK+RNIRDFT+SSSSSSES+STAGRR+S
Sbjct: 61  MRTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYS 120

Query: 121 SSESEFLSRALHRPKPKPKPIKTTTWAQTEIISPNPKHENGFVKSKSKASKIYHDLKKVK 180
           +SESE LSRALHR     KPIKTT W QTE  +   KHENG VK+KSKASKIYHDLKKVK
Sbjct: 121 ASESELLSRALHR----QKPIKTTPWTQTE--TQKAKHENGLVKTKSKASKIYHDLKKVK 180

Query: 181 QPISPGGRLASFLNSLFNGGSPKTKQKISNASSINSTNFDYDMSRKSKSQQGSTSTCSSA 240
           QPISPGGRLA+FLNSLFNGGSPKTKQK+ N S+ N  + DYDM  K+KSQ    STCSSA
Sbjct: 181 QPISPGGRLANFLNSLFNGGSPKTKQKMPNPST-NPRSMDYDMPTKAKSQ---GSTCSSA 240

Query: 241 SSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKLEEPIMKKGNLKSYG 300
           SSFSRSCLSKTPSSRGN+KRSVRFCPVS IVDEDC+  GHK L KLEEPIMKKG LKSYG
Sbjct: 241 SSFSRSCLSKTPSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKG-LKSYG 300

Query: 301 NFETEKVKTEHEMK------ISYEDED----DDDALSCSSSDLFELDNLSVIGIERYREE 360
            FETE+VKTEH  K        YEDED    +DDA+SCSSSDLFELDNLSVIG+ERYREE
Sbjct: 301 KFETEEVKTEHPRKSKGNEGFEYEDEDEEEEEDDAVSCSSSDLFELDNLSVIGMERYREE 360

Query: 361 LPVYETTHFKTNCAIANGLVL 372
           LPVYETT+FKTNCAIA GLV+
Sbjct: 361 LPVYETTNFKTNCAIAKGLVV 369

BLAST of Lsi07G006100 vs. NCBI nr
Match: XP_038903204.1 (protein BIG GRAIN 1-like B [Benincasa hispida])

HSP 1 Score: 654.4 bits (1687), Expect = 5.7e-184
Identity = 349/376 (92.82%), Postives = 358/376 (95.21%), Query Frame = 0

Query: 1   MDLW-DHSFHSRDSDHLQQRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKT 60
           MDLW DHSFH+ D+     RRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLI YTQKT
Sbjct: 1   MDLWADHSFHTTDN---PDRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLILYTQKT 60

Query: 61  TVRTKQSI-AAARTEDDETLKFRRACMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGRR 120
           T+RTKQSI +AARTEDDETLKF RAC+IDKWMDKKKLRNIRDF+LSSSSSSESSSTAGRR
Sbjct: 61  TLRTKQSISSAARTEDDETLKFHRACIIDKWMDKKKLRNIRDFSLSSSSSSESSSTAGRR 120

Query: 121 FSSSESEFLSRALHRPKPKPKPIKTTTWAQTEIISPNPKHENGFVKSKSKASKIYHDLKK 180
           FSSSESEFLSRALHRPKPKPKPIKTTTWAQTEIISP PKHENGFVKSKSKASKIYHDLKK
Sbjct: 121 FSSSESEFLSRALHRPKPKPKPIKTTTWAQTEIISPKPKHENGFVKSKSKASKIYHDLKK 180

Query: 181 VKQPISPGGRLASFLNSLFNGGSPKTKQKISNASSINSTNFDYDMSRKSKSQQGSTSTCS 240
           VKQPISPGGRLASFLNSLFNGGSPKTKQKISN+SSINSTNFDYDMSRKSKSQQ STSTCS
Sbjct: 181 VKQPISPGGRLASFLNSLFNGGSPKTKQKISNSSSINSTNFDYDMSRKSKSQQVSTSTCS 240

Query: 241 SASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKLEEPIMKKGNLKS 300
           SASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCG KILHKLEEPIMKKGNLKS
Sbjct: 241 SASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGRKILHKLEEPIMKKGNLKS 300

Query: 301 YGNFETEKVKTEHEMKISY---EDEDDDDALSCSSSDLFELDNLSVIGIERYREELPVYE 360
           Y NFETEKVKTEHEMKI+Y   ED+DDDDALSCSSSDLFELDNLSVIGIERYREELPVYE
Sbjct: 301 YRNFETEKVKTEHEMKINYELDEDDDDDDALSCSSSDLFELDNLSVIGIERYREELPVYE 360

Query: 361 TTHFKTNCAIANGLVL 372
           TTHF TNCAIANGLVL
Sbjct: 361 TTHFNTNCAIANGLVL 373

BLAST of Lsi07G006100 vs. NCBI nr
Match: XP_008464005.1 (PREDICTED: protein BIG GRAIN 1-like B [Cucumis melo])

HSP 1 Score: 573.9 bits (1478), Expect = 9.8e-160
Identity = 321/385 (83.38%), Postives = 336/385 (87.27%), Query Frame = 0

Query: 1   MDLWDHSFHSRDSDHLQQRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTT 60
           MDLWDHS    D   LQQ RRSRR+NPSFSSSLLDAIYRSIDESN+QPEDHLIFYT  TT
Sbjct: 1   MDLWDHS----DPHLLQQPRRSRRDNPSFSSSLLDAIYRSIDESNSQPEDHLIFYTHNTT 60

Query: 61  VRTKQSIAAARTEDDETLKFRRACMIDKWMD-KKKLRNIRDFTLSSSSSSESSSTAGRRF 120
             T   + A  ++D E+LKFR   MID W+D KKKLRNIRDFTLSSSSSSESSSTAGRRF
Sbjct: 61  KHT--ILPATVSQDHESLKFR---MIDTWIDKKKKLRNIRDFTLSSSSSSESSSTAGRRF 120

Query: 121 SSSESEFLSRALHRP---KPKPKPIKTTTWAQTEIISPNPKHENGFVKSKSKASKIYHDL 180
           SSSE+EFLSRALHRP   KPKPKPIKT TW QTEII+PNPKH+NGFVKSKSKASKIYHDL
Sbjct: 121 SSSETEFLSRALHRPTKLKPKPKPIKTNTWPQTEIITPNPKHDNGFVKSKSKASKIYHDL 180

Query: 181 KKVKQPISPGGRLASFLNSLFNGGSPKTKQKISNAS-SINSTNFDYDMSRKSKSQQGSTS 240
           KKVKQPISPG RLASFLNSLFNGGSPK KQKIS+++ SINST FDYDMSRKSKSQQGSTS
Sbjct: 181 KKVKQPISPGARLASFLNSLFNGGSPKAKQKISSSTCSINSTKFDYDMSRKSKSQQGSTS 240

Query: 241 TCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKLEEPIMKKGN 300
           TCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHK EEPIMKKGN
Sbjct: 241 TCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKSEEPIMKKGN 300

Query: 301 L-KSYGNFETEKVKTEHEMKISYE--------DEDDDDALSCSSSDLFELDNLSVIGIER 360
           L KS GN E EKVKTE  MKI+YE        D+DDDDALSCSSSDLFELDNLSVIGIER
Sbjct: 301 LKKSNGNLEREKVKTEDMMKINYEEEEEEDDDDDDDDDALSCSSSDLFELDNLSVIGIER 360

Query: 361 YREELPVYETTHFKTNCAIANGLVL 372
           YREELPVYETTHFKTNCAIA GLVL
Sbjct: 361 YREELPVYETTHFKTNCAIAKGLVL 376

BLAST of Lsi07G006100 vs. NCBI nr
Match: XP_004141159.1 (protein BIG GRAIN 1-like B [Cucumis sativus])

HSP 1 Score: 572.8 bits (1475), Expect = 2.2e-159
Identity = 319/382 (83.51%), Postives = 337/382 (88.22%), Query Frame = 0

Query: 1   MDLWDHSFHSRDSDHLQQRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTT 60
           MDLWDHS        LQQ RRSRR+NPSFSSSLLDAIYRSIDESN+QPE+HLIFY+ KTT
Sbjct: 1   MDLWDHS----HPHLLQQPRRSRRDNPSFSSSLLDAIYRSIDESNSQPEEHLIFYSHKTT 60

Query: 61  VRTKQSIAAAR-TEDDETLKFRRACMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGRRF 120
           + TK SI     T+D E+L FR   MID WMDKK+LRNIRDFTLSSSSSSESSSTAGRRF
Sbjct: 61  LTTKHSILPRTVTQDPESLNFR---MIDSWMDKKQLRNIRDFTLSSSSSSESSSTAGRRF 120

Query: 121 SSSESEFLSRALHRP-KPKPKPIKTTTWAQTEIISPNPKHENGFVKSKSKASKIYHDLKK 180
           SSSE+EFLSR LHRP K KPKPIKT TWAQTEII+PNPKHENGFVKSKSKASKIYHDLKK
Sbjct: 121 SSSETEFLSRPLHRPTKLKPKPIKTNTWAQTEIITPNPKHENGFVKSKSKASKIYHDLKK 180

Query: 181 VKQPISPGGRLASFLNSLFNGGSPKTKQKISNAS-SINSTNFDYDMSRKSKSQQGSTSTC 240
           VKQPISPG RLASFLNSLFNGGSPKTKQKIS+++ SINST FDYDMSRKSKSQQGSTSTC
Sbjct: 181 VKQPISPGARLASFLNSLFNGGSPKTKQKISSSTCSINSTKFDYDMSRKSKSQQGSTSTC 240

Query: 241 SSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKLEEPIMKKGNL- 300
           SSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDC+PCGHK LHK EEPIMKKGNL 
Sbjct: 241 SSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCRPCGHKFLHKSEEPIMKKGNLK 300

Query: 301 KSYGNFE-TEKVKTEHEMKISYE------DEDDDDALSCSSSDLFELDNLSVIGIERYRE 360
           KSYGN +  +KVKTE  MK++YE      DEDDDDALSCSSSDLFELDNLSVIGIERYRE
Sbjct: 301 KSYGNLKRDQKVKTEDMMKMNYEDEEEDDDEDDDDALSCSSSDLFELDNLSVIGIERYRE 360

Query: 361 ELPVYETTHFKTNCAIANGLVL 372
           ELPVYETTHFKTNCAIA GLVL
Sbjct: 361 ELPVYETTHFKTNCAIAKGLVL 375

BLAST of Lsi07G006100 vs. NCBI nr
Match: KAG6581818.1 (Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 556.2 bits (1432), Expect = 2.1e-154
Identity = 302/376 (80.32%), Postives = 327/376 (86.97%), Query Frame = 0

Query: 1   MDLWDHSFHSRDSD---HLQQRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQ 60
           MDLWD+SF   D D   H  QRRRSRRENPSFSSSLLDAIYRSID+SNAQ ED LIFYTQ
Sbjct: 1   MDLWDNSFQREDPDPHQHHHQRRRSRRENPSFSSSLLDAIYRSIDDSNAQGEDQLIFYTQ 60

Query: 61  KTTVRTKQSIAAARTEDDETLKFRRACMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGR 120
           KTT RTKQSI+AA+TED +TL+FRRA MI+ WMD KK++ +RDFT+S+SSSSESSSTAGR
Sbjct: 61  KTTTRTKQSISAAKTEDHQTLQFRRASMIENWMDNKKIKILRDFTVSTSSSSESSSTAGR 120

Query: 121 RFSSSESEFLSRALHR--PKPKPKPIKTTTWAQTEIISPNPKHENGFVKSKSKASKIYHD 180
           R SSSESEFLSR LHR  PKPKPKPIKTTTW+QTEII PNP HENGFVKSK KASKIYHD
Sbjct: 121 RLSSSESEFLSRTLHRPKPKPKPKPIKTTTWSQTEII-PNPNHENGFVKSKFKASKIYHD 180

Query: 181 LKKVKQPISPGGRLASFLNSLFNGGSPKTKQKISNASSINSTNFDYDMSRKSKSQQGSTS 240
           LKKVKQPISPGGRLASFLNSLFNGGSPK KQKISN  S NSTN +Y+  RKSKSQQGS+S
Sbjct: 181 LKKVKQPISPGGRLASFLNSLFNGGSPKAKQKISNTCSNNSTNLEYEAPRKSKSQQGSSS 240

Query: 241 TCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKLEEPIMKKGN 300
           TCSSASSFSRSCL KTPSSRGNIKRSVRFCPVSVIVDEDC+PCGHK+L+KLEEPI KKG 
Sbjct: 241 TCSSASSFSRSCLIKTPSSRGNIKRSVRFCPVSVIVDEDCRPCGHKMLNKLEEPIGKKGK 300

Query: 301 LKSYGNFETEKVKTEHEMKISYEDEDDDDALSCSSSDLFELDNLSVIGIERYREELPVYE 360
           L      ETE++KTE       +DEDDDD LSCSSSDLFELDNLSVIG+ERYR+ELPVYE
Sbjct: 301 L------ETEEMKTEE------DDEDDDDRLSCSSSDLFELDNLSVIGMERYRDELPVYE 360

Query: 361 TTHFKTNCAIANGLVL 372
           TTHF TNCAIANGL+L
Sbjct: 361 TTHFNTNCAIANGLLL 363

BLAST of Lsi07G006100 vs. NCBI nr
Match: KAG7018266.1 (Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 555.8 bits (1431), Expect = 2.8e-154
Identity = 301/376 (80.05%), Postives = 326/376 (86.70%), Query Frame = 0

Query: 1   MDLWDHSFHSRDSD---HLQQRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQ 60
           MDLWD+SF   D D   H  QRRRSRRENPSFSSSLLDAIYRSID+SNAQ ED LIFYTQ
Sbjct: 1   MDLWDNSFQREDPDPHHHHHQRRRSRRENPSFSSSLLDAIYRSIDDSNAQGEDQLIFYTQ 60

Query: 61  KTTVRTKQSIAAARTEDDETLKFRRACMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGR 120
           KTT RTKQSI+AA+TED +TL+FRRA MI+ WMD KK++ +RDFT+S+SSSSESSSTAGR
Sbjct: 61  KTTTRTKQSISAAKTEDHQTLQFRRASMIENWMDNKKIKTLRDFTVSTSSSSESSSTAGR 120

Query: 121 RFSSSESEFLSRALHR--PKPKPKPIKTTTWAQTEIISPNPKHENGFVKSKSKASKIYHD 180
           R SSSESEFLSR LHR  PKPKPKPIKTTTW+QTEII PNP HENGFVKSK KASKIYHD
Sbjct: 121 RLSSSESEFLSRTLHRPKPKPKPKPIKTTTWSQTEII-PNPNHENGFVKSKFKASKIYHD 180

Query: 181 LKKVKQPISPGGRLASFLNSLFNGGSPKTKQKISNASSINSTNFDYDMSRKSKSQQGSTS 240
           LKKVKQPISPGGRLASFLNSLFNGGSPK KQKISN  S NSTN +Y+  RKSKSQQGS+S
Sbjct: 181 LKKVKQPISPGGRLASFLNSLFNGGSPKAKQKISNTCSNNSTNLEYEAPRKSKSQQGSSS 240

Query: 241 TCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKLEEPIMKKGN 300
           TCSSASSFSRSCL KTPSSRGNIKRSVRFCPVSVIVDEDC+PCGHK+L+KLEEPI KKG 
Sbjct: 241 TCSSASSFSRSCLIKTPSSRGNIKRSVRFCPVSVIVDEDCRPCGHKMLNKLEEPIGKKGK 300

Query: 301 LKSYGNFETEKVKTEHEMKISYEDEDDDDALSCSSSDLFELDNLSVIGIERYREELPVYE 360
           L      ETE++KTE       +DEDDDD LSCSSSDLFELDNLS IG+ERYR+ELPVYE
Sbjct: 301 L------ETEEMKTEE------DDEDDDDRLSCSSSDLFELDNLSAIGMERYRDELPVYE 360

Query: 361 TTHFKTNCAIANGLVL 372
           TTHF TNCAIANGL+L
Sbjct: 361 TTHFNTNCAIANGLLL 363

BLAST of Lsi07G006100 vs. TAIR 10
Match: AT1G54200.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1084 Blast hits to 581 proteins in 136 species: Archae - 0; Bacteria - 72; Metazoa - 212; Fungi - 78; Plants - 102; Viruses - 0; Other Eukaryotes - 620 (source: NCBI BLink). )

HSP 1 Score: 225.3 bits (573), Expect = 7.9e-59
Identity = 175/397 (44.08%), Postives = 217/397 (54.66%), Query Frame = 0

Query: 1   MDLWDHSFHSRDSDHLQQRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTT 60
           MD WD      D  H  + RR  R +PSFSS+LLD IYRSID+S+           +KT 
Sbjct: 1   MDPWDTYNIPLDHQHRLRNRRDHR-HPSFSSTLLDQIYRSIDDSSTNSSS-----MRKTK 60

Query: 61  VRTKQSIAAARTEDDETLKFRRACMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGRRFS 120
            + ++    +    D+   F R+         K L+ I       SSSS S S+    FS
Sbjct: 61  HQNREDTRVSANRRDD---FNRS---------KNLKTIEPVFFKHSSSSSSDSSG---FS 120

Query: 121 SSESE-FLSRALHRPK-PKPKPIKTTT-----WAQTEIISPNPKHENG-FVKSKSKASKI 180
           SSES+ F  R+   P    PKPI+TT        Q    + + K E+G F+K+KSKA KI
Sbjct: 121 SSESDYFYRRSKSSPAISHPKPIRTTVERFERSPQNHRPNSSNKQEHGSFLKTKSKALKI 180

Query: 181 YHDLKKVKQPISPGGRLASFLNSLFNGGSPKTKQKISNASSINSTNFDYDMSRKSKSQQG 240
           Y DLKKVKQPISPGGRLA+FLNS+F G          N   +N  N     +  + +   
Sbjct: 181 YSDLKKVKQPISPGGRLATFLNSIFTGA--------GNTKKLNKINTTVTSTTAAAAAAS 240

Query: 241 STSTCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHK----------- 300
           ST+TCSSASSFSRSCLSKTPSS    KRSVRFCPV+VI DED     +K           
Sbjct: 241 STTTCSSASSFSRSCLSKTPSSSEKSKRSVRFCPVNVIFDEDSSKYNNKNNKVYGNNERE 300

Query: 301 ---ILHKLEEPIMKKGN---------LKSYGNFETEKVKTEHEMKISYEDEDDDDALSCS 360
              I H LE  +M++           L+SY     E ++   E     E++DDDDALSC+
Sbjct: 301 YESIRHTLENRVMEENRRVIEAAKELLRSYQKKNKEVIEVSVE---DDEEDDDDDALSCT 360

Query: 361 SSDLFELDNLSVIGIERYREELPVYETTHFKTNCAIA 367
           SSDLFELDNLS IGI+RYREELPVYETT   TN  I+
Sbjct: 361 SSDLFELDNLSAIGIDRYREELPVYETTRLNTNRIIS 365

BLAST of Lsi07G006100 vs. TAIR 10
Match: AT3G13980.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 1485 Blast hits to 418 proteins in 98 species: Archae - 0; Bacteria - 6; Metazoa - 246; Fungi - 61; Plants - 107; Viruses - 6; Other Eukaryotes - 1059 (source: NCBI BLink). )

HSP 1 Score: 215.7 bits (548), Expect = 6.3e-56
Identity = 169/375 (45.07%), Postives = 211/375 (56.27%), Query Frame = 0

Query: 22  SRRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTTVRTKQSIAAARTEDDE--TLK 81
           S   NPSFSS+LLD IYRSID+S+  P    I    K      Q   A+  ED E   + 
Sbjct: 12  SHHRNPSFSSTLLDQIYRSIDDSSPPPPLESI----KKKKHHHQQRNASLHEDREISPIY 71

Query: 82  FRRACMIDKWMDKKKLRNIRDFTLSSS-----SSSESSSTAGRRFSSSESEFLSRALHRP 141
            RR+   D    ++K   +R    SSS     SSSES S  GR  SS+     SR     
Sbjct: 72  HRRSIAADFERSRRKTDFLRHSNSSSSDSSGFSSSESDSFHGRSKSSASPPSSSR----- 131

Query: 142 KPKPKPIKTTTWAQTEIISPNPKHENGFVKSKSKASKIYHDLKKVKQPISPGGRLASFLN 201
             +PKPI+T++   +  +   PK   GF+++KSKA KIY DLKKVKQPISPGGRLA+FLN
Sbjct: 132 -QQPKPIRTSSVDHSSAVQ-KPKELGGFLRTKSKALKIYSDLKKVKQPISPGGRLATFLN 191

Query: 202 SLFNGG--SPKTKQKISNASSINSTNFDYDMSRKSKSQQGSTSTCSSASSFSRSCLSKTP 261
           SLF     +PK  +K +  + +   +              S+STCSSASSFSRSCLSKTP
Sbjct: 192 SLFTNAATNPKKHKKTTTVAVVEEPH--------------SSSTCSSASSFSRSCLSKTP 251

Query: 262 SSRGNIKRSVRFCPVSVIVDED---CQPCGHKILHKLEEPIMK------------KGNLK 321
           SS G  KRSVRFCPV+VI+DED     P  +      +    K            K  L+
Sbjct: 252 SSSGKSKRSVRFCPVNVILDEDSSFTMPYAYNNERLYDNNEAKRVEEHRRVIQAAKDLLR 311

Query: 322 SYGNFETEKVKTEHEMKISYEDEDDDDALSCSSSDLFELDNLSVIGIERYREELPVYETT 372
           +Y N    KV T +   +  E++D+DDA SC+SSDLFEL+NLS IGIERYREELPVYETT
Sbjct: 312 TYHN--KNKVTTTNINNV--EEDDEDDAASCASSDLFELENLSAIGIERYREELPVYETT 357

BLAST of Lsi07G006100 vs. TAIR 10
Match: AT3G42800.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 853 Blast hits to 340 proteins in 98 species: Archae - 0; Bacteria - 94; Metazoa - 145; Fungi - 62; Plants - 95; Viruses - 0; Other Eukaryotes - 457 (source: NCBI BLink). )

HSP 1 Score: 134.4 bits (337), Expect = 1.8e-31
Identity = 133/374 (35.56%), Postives = 185/374 (49.47%), Query Frame = 0

Query: 23  RRENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTTVRTKQSIAAARTEDDETLKFRR 82
           R+  PSFSSS+LD++YRSIDES+    D  +  +    V +  S  +   +DD+    RR
Sbjct: 6   RKRTPSFSSSVLDSVYRSIDESDGLQSD--LKGSINENVSSSSSSPSPNKKDDKLTTLRR 65

Query: 83  ACMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGRRFSSSESEFLSRALHRPKPKPKPIK 142
           A      MD++     R  T +++SS  SS      FSSSE+E         K   +  +
Sbjct: 66  AI-----MDEEHWLYARSSTTTTNSSDSSS------FSSSEAESYRTKRRLRKLAEQGKR 125

Query: 143 TTTWAQTEIISPNPKHENGFVKS----KSKASKIYHDLKKVKQPISPGGRLASFLNSLFN 202
           +    Q    +        F KS    K KA KI  +LK+ KQP+SPG RL SFLNS+F 
Sbjct: 126 SGDERQRTKRTVMDNDSRLFSKSDDDKKPKAVKIIEELKRSKQPVSPGARLTSFLNSIFQ 185

Query: 203 GGSPKTKQKISNASSINSTNFDYDMSRKSKSQQGSTSTCSSASSFSRSCLSKTPSSRGNI 262
             + K K       S+  T                  + SS S FSR+  +KT ++  N 
Sbjct: 186 SNAKKVK-----LCSVGKTT--------------DVKSSSSKSCFSRT-RNKTDNNNNNC 245

Query: 263 K---RSVRFCPVSVIVDEDCQPCGHKILHKLEEPIMKKGNLKSYGNFETEKVKT-EHEMK 322
           K   RS+RF PV V +D DC+    K + ++ +PI +    KS      E++KT +H  +
Sbjct: 246 KKLERSIRFYPVRVTIDGDCRDYAQKHITRVRKPIPEFTAKKSV----KEEIKTNDHHTE 305

Query: 323 IS----------------YE-DEDDDDALSCSSSDLFELDNLSVIGIERYREELPVYETT 372
            +                YE  E+++DA S SSSDLFELD+   IG+ RY +ELPVYETT
Sbjct: 306 FTCITRNIGLKDFVRSNKYEGKEEEEDAWSHSSSDLFELDSYR-IGMGRYLKELPVYETT 341

BLAST of Lsi07G006100 vs. TAIR 10
Match: AT5G12050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 119.0 bits (297), Expect = 8.0e-27
Identity = 138/385 (35.84%), Postives = 179/385 (46.49%), Query Frame = 0

Query: 24  RENPSFSSSLLDAIYRSIDESNAQPEDHLIFYTQKTTVRTKQSIAAARTEDDETLKFRRA 83
           + NPSFSS+LLD IY SID    + + ++       T   KQSI      D         
Sbjct: 5   KRNPSFSSTLLDEIYNSIDPKTQKTQPYV---GSVNTTTKKQSIVTRSVPD--------- 64

Query: 84  CMIDKWMDKKKLRNIRDFTLSSSSSSESSSTAGRRFSSSESEFL-------SRALHRPKP 143
                    +K+   R F   SSSS  +SS     FSSS++E         SR L     
Sbjct: 65  ---------RKIHRDRFFGSVSSSSDSNSSI----FSSSDTELTHGKKTTSSRPLCFGPS 124

Query: 144 KPKPIK------------TTTWAQTEIISPNPK---HENGFVKSKSKAS-KIYHDLKKVK 203
           K KP K            T  +   +  S  PK   H+  +  ++++ S K   + KK K
Sbjct: 125 KTKPRKTEDKTLFHQNRATRVYDDYDYASDVPKFNRHDENWENTRNRRSVKSSGNQKKPK 184

Query: 204 QPISPGGRLASFLNSLFNGGSPKTKQKISNASSINSTNFDYDMS----RKSKSQQGSTST 263
            P SPGGR+ +FLNSLF+  S     K SNA         YD S    + S     ST+T
Sbjct: 185 TPASPGGRIVNFLNSLFSNNS-----KQSNAVKSYPRKTSYDDSAYVRKTSNDYHSSTTT 244

Query: 264 CSSASSFSRSCLSK-TPSSRGNIKRSVRFCPVSVIV-------DEDCQPCGH---KILHK 323
           CSSASSFSRSC++K    S G IKRSVRF PV+VIV       +ED    G+    +   
Sbjct: 245 CSSASSFSRSCMNKGYEKSSGRIKRSVRFSPVNVIVPESFTSKEEDYFSNGNARKSVKKN 304

Query: 324 LE-------EPIMKKGNLKSYGNFETEKVKTE--HEMKISYEDEDDDDALSCSSSDLFEL 358
           +E       E I ++     + N E   VKT    + +   ED+DDDD  S SSSDLFEL
Sbjct: 305 VEDGGRRSVEEIAREFLRDYHKNHENSLVKTNGFEDYEDDDEDDDDDDVASDSSSDLFEL 356

BLAST of Lsi07G006100 vs. TAIR 10
Match: AT1G69160.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 173 Blast hits to 172 proteins in 54 species: Archae - 0; Bacteria - 0; Metazoa - 25; Fungi - 33; Plants - 84; Viruses - 2; Other Eukaryotes - 29 (source: NCBI BLink). )

HSP 1 Score: 55.8 bits (133), Expect = 8.3e-08
Identity = 66/206 (32.04%), Postives = 97/206 (47.09%), Query Frame = 0

Query: 180 KQPISPGGRLASFLNSLFN-GGSPKTKQKISNASSINSTNFDYD------MSRK------ 239
           KQP SPGG++ASFLNSLF+  GS K K K  + +       + +      M R+      
Sbjct: 114 KQPSSPGGKIASFLNSLFHQAGSKKNKSKSKSKTKPTDPEVEEEIPGGGWMRRRRRSSIS 173

Query: 240 ---SKSQQGSTSTCSSASSFSRSCLSKTPSS-----------RGNIKRSVRFCPVSVIVD 299
              S S+  ST+T ++ASS S+S +S + S              N K+ + +   +  V 
Sbjct: 174 HFFSSSRSTSTTTTTTASSSSKSLISSSSSGFRTPPPYLNTPTKNYKQFLNYTSATKQVG 233

Query: 300 EDCQPCGHKILHKLEEPIMKKGNLKSYGNFETEKVKTEHEMKISYEDEDDDDALSCSSSD 359
           E+ +   +K    L+E +    +L       ++    + + +I  E E DD   S SSSD
Sbjct: 234 EE-ETKTNKEYSWLDEKLKVMESLSENQRIWSDDEDIDDDRRIKREGE-DDGMESDSSSD 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SLL21.1e-5744.08Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=... [more]
Q9LVK28.9e-5545.07Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=... [more]
Q9M2B32.6e-3035.56Protein BIG GRAIN 1-like C OS=Arabidopsis thaliana OX=3702 GN=At3g42800 PE=2 SV=... [more]
Q9LYH01.1e-2535.84Protein BIG GRAIN 1-like D OS=Arabidopsis thaliana OX=3702 GN=At5g12050 PE=2 SV=... [more]
Q10R092.8e-2433.80Protein BIG GRAIN 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BG1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CKK14.7e-16083.38protein BIG GRAIN 1-like B OS=Cucumis melo OX=3656 GN=LOC103501995 PE=3 SV=1[more]
A0A6J1IZW58.6e-15479.31protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111479960 PE=3 SV=1[more]
A0A6J1GWE33.1e-15178.25protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111457754 PE=3 SV... [more]
A0A6J1KB464.8e-14476.80protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111493335 PE=3 SV=1[more]
A0A6J1GAP73.4e-14275.59protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111452438 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_038903204.15.7e-18492.82protein BIG GRAIN 1-like B [Benincasa hispida][more]
XP_008464005.19.8e-16083.38PREDICTED: protein BIG GRAIN 1-like B [Cucumis melo][more]
XP_004141159.12.2e-15983.51protein BIG GRAIN 1-like B [Cucumis sativus][more]
KAG6581818.12.1e-15480.32Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7018266.12.8e-15480.05Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT1G54200.17.9e-5944.08unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G13980.16.3e-5645.07unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G42800.11.8e-3135.56unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G12050.18.0e-2735.84unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G69160.18.3e-0832.04unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 222..256
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 102..127
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 102..166
NoneNo IPR availablePANTHERPTHR33541:SF2PROTEIN BIG GRAIN 1-LIKE Acoord: 15..371
IPR039621Protein BIG GRAIN 1-likePANTHERPTHR33541PROTEIN BIG GRAIN 1-LIKE A-RELATEDcoord: 15..371

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G006100.1Lsi07G006100.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
cellular_component GO:0005886 plasma membrane