Lsi07G004900 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi07G004900
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	2-oxoglutarate-dependent dioxygenase AOP2-like
Location	chr07: 5178106 .. 5178516 (+)
RNA-Seq Expression	Lsi07G004900
Synteny	Lsi07G004900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATTCAAACTTTAGCAAAGTGTGAAGTTCCTTTGATAAAATTCTCCATTGAATTAAATGATTATAATTTTGATGATCCTCAAAGCACCAAATGGTTAGAAAGATGCAGCCAAGTGAGAGTTGCTCTTGAAGAATATGGCTGTTTCTTAGCTGAGTATGATGAGATCAGTGCCCCACAAGAGCTTTATGAAGCAATTTTGAAGGGCTTGAAAGTGCTTTTTGATTTGCCATTAGAAACAAAAATGAAGAATGTTTCTGATAAGCCATACCATGGATTTTTGGACCATACGACTCCCTTTGTTCTTCCTCTTCATCAAAGCCTTGGCATTGAAAATGCAACATCTTTTCAAGCTGTTGAATCTTTTGTCAACCTCTTCTGGCCTTCTGGAAATCATCATTTCTGGTAA

mRNA sequence

ATGGAAATTCAAACTTTAGCAAAGTGTGAAGTTCCTTTGATAAAATTCTCCATTGAATTAAATGATTATAATTTTGATGATCCTCAAAGCACCAAATGGTTAGAAAGATGCAGCCAAGTGAGAGTTGCTCTTGAAGAATATGGCTGTTTCTTAGCTGAGTATGATGAGATCAGTGCCCCACAAGAGCTTTATGAAGCAATTTTGAAGGGCTTGAAAGTGCTTTTTGATTTGCCATTAGAAACAAAAATGAAGAATGTTTCTGATAAGCCATACCATGGATTTTTGGACCATACGACTCCCTTTGTTCTTCCTCTTCATCAAAGCCTTGGCATTGAAAATGCAACATCTTTTCAAGCTGTTGAATCTTTTGTCAACCTCTTCTGGCCTTCTGGAAATCATCATTTCTGGTAA

Coding sequence (CDS)

ATGGAAATTCAAACTTTAGCAAAGTGTGAAGTTCCTTTGATAAAATTCTCCATTGAATTAAATGATTATAATTTTGATGATCCTCAAAGCACCAAATGGTTAGAAAGATGCAGCCAAGTGAGAGTTGCTCTTGAAGAATATGGCTGTTTCTTAGCTGAGTATGATGAGATCAGTGCCCCACAAGAGCTTTATGAAGCAATTTTGAAGGGCTTGAAAGTGCTTTTTGATTTGCCATTAGAAACAAAAATGAAGAATGTTTCTGATAAGCCATACCATGGATTTTTGGACCATACGACTCCCTTTGTTCTTCCTCTTCATCAAAGCCTTGGCATTGAAAATGCAACATCTTTTCAAGCTGTTGAATCTTTTGTCAACCTCTTCTGGCCTTCTGGAAATCATCATTTCTGGTAA

Protein sequence

MEIQTLAKCEVPLIKFSIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWPSGNHHFW

Homology

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BLAST of Lsi07G004900 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 2.6e-20
Identity = 50/106 (47.17%), Postives = 67/106 (63.21%), Query Frame = 0


Query: 27  DPQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILKGLKVLFDLPLETKMKNV 86
           +P S+KW E  S VR ALE++GCF A YD++S   EL E+I+K ++ LF LP+ETK +NV
Sbjct: 19  EPGSSKWAEVRSDVRKALEDFGCFEASYDKVSL--ELQESIMKTMEELFALPVETKQRNV 78

Query: 87  SDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWPSGN 133
             KPY G+L+H       L +SLGI NA   + V  F    WP G+
Sbjct: 79  CPKPYVGYLNHNN-----LSESLGISNANILENVNEFTQQLWPDGD 117

[Close]

BLAST of Lsi07G004900 vs. ExPASy Swiss-Prot
Match: Q9ZTA2 (Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 4.4e-20
Identity = 49/106 (46.23%), Postives = 67/106 (63.21%), Query Frame = 0


Query: 27  DPQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILKGLKVLFDLPLETKMKNV 86
           +P S+KW E  S VR ALE++GCF A YD++S   EL E+I+K ++ LF LP+ETK +NV
Sbjct: 19  EPGSSKWAEVRSDVRKALEDFGCFEASYDKVSL--ELQESIMKTMEELFALPVETKQRNV 78

Query: 87  SDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWPSGN 133
             KPY G+L+H       L +SLGI NA   + +  F    WP G+
Sbjct: 79  CPKPYVGYLNHNN-----LSESLGISNANILENINEFTQQLWPHGD 117

[Close]

BLAST of Lsi07G004900 vs. ExPASy Swiss-Prot
Match: Q944Z9 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 9.9e-20
Identity = 48/106 (45.28%), Postives = 67/106 (63.21%), Query Frame = 0


Query: 27  DPQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILKGLKVLFDLPLETKMKNV 86
           +P S+KW E  S VR AL+++GCF A YD++S   EL E+I+K ++ LF LP+ETK +NV
Sbjct: 19  EPGSSKWAEVRSDVRKALQDFGCFEASYDKVSL--ELQESIMKTMEELFALPVETKQRNV 78

Query: 87  SDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWPSGN 133
             KPY G+L+H       L +SLGI NA   + +  F    WP G+
Sbjct: 79  CPKPYVGYLNHNN-----LSESLGISNANILENINEFTQQLWPHGD 117

[Close]

BLAST of Lsi07G004900 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.4e-18
Identity = 46/120 (38.33%), Postives = 72/120 (60.00%), Query Frame = 0


Query: 12  PLIKFSIELNDYNFDD--PQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILK 71
           P + F + + D++  +  P S+KW E  + V  ALE+YGCF A +D++S   EL  ++ +
Sbjct: 8   PSVSFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSV--ELNRSVFE 67

Query: 72  GLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWP 130
            ++ LF+LP+ TK +NVS KP+HG+L H       L++SLGI +A   + V  F    WP
Sbjct: 68  AMEDLFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWP 119

[Close]

BLAST of Lsi07G004900 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 5.4e-18
Identity = 46/120 (38.33%), Postives = 73/120 (60.83%), Query Frame = 0


Query: 12  PLIKFSIELNDYNFDD--PQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILK 71
           P + F + + D++  +  P S+KW E  + V  ALE+YGCF A +D++S   EL  ++ +
Sbjct: 8   PSVSFQLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSV--ELNRSVFE 67

Query: 72  GLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWP 130
            ++ LF+LP+ TK +NVS KP+HG+L H       L++SLGI++A   + V  F    WP
Sbjct: 68  AMEDLFELPIPTKQRNVSSKPFHGYLCHN------LYESLGIDDANVLEKVNDFTQQLWP 119

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BLAST of Lsi07G004900 vs. ExPASy TrEMBL
Match: A0A5D3DAA8 (2-oxoglutarate-dependent dioxygenase AOP2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00070 PE=4 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.6e-44
Identity = 96/148 (64.86%), Postives = 108/148 (72.97%), Query Frame = 0


Query: 1   MEIQTLAKCEVPLIKF-SIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDE--- 60
           M I+ LAK EVP+IK  +I+  D + D   S  WLER  QVR ALEEYGCFL EYD+   
Sbjct: 1   MGIECLAKLEVPIIKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDES 60

Query: 61  -----ISAPQEL---YEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQS 120
                 S+  +L   YE I KGLK LFD PLETKMKNVSDKPYHGFLD   PFVLPLHQS
Sbjct: 61  SRCCNSSSSSKLDSNYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQS 120

Query: 121 LGIENATSFQAVESFVNLFWPSGNHHFW 137
           LGIENA+S++AV+SFVNL WPSGNHHFW
Sbjct: 121 LGIENASSYEAVQSFVNLLWPSGNHHFW 148

[Close]

BLAST of Lsi07G004900 vs. ExPASy TrEMBL
Match: A0A6J1GA17 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111452291 PE=3 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 3.9e-43
Identity = 90/136 (66.18%), Postives = 104/136 (76.47%), Query Frame = 0


Query: 1   MEIQTLAKCEVPLIKFSIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYD-EISA 60
           M  ++  K EVP+I FSIE    N   P+STKW+E C+ VR ALEEYGCFLAEY+ ++  
Sbjct: 1   MGFRSSTKPEVPVINFSIE----NL-GPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQ 60

Query: 61  PQELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQA 120
             EL +AI  GL+ LFDLPL+TKMKNVSDKPYHGFLDH  PFVLPLH+S GIENA SF+ 
Sbjct: 61  EVELDDAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEG 120

Query: 121 VESFVNLFWPSGNHHF 136
           VESFV L WPSGNHHF
Sbjct: 121 VESFVKLMWPSGNHHF 131

[Close]

BLAST of Lsi07G004900 vs. ExPASy TrEMBL
Match: A0A0A0LBU1 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G555690 PE=4 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.5e-42
Identity = 96/148 (64.86%), Postives = 107/148 (72.30%), Query Frame = 0


Query: 1   MEIQTLAKCEVPLIKFSIELNDYNFDDPQSTK-WLERCSQVRVALEEYGCFLAEYDE--- 60
           M I  LAK EVP+IK S      +  DP+S K WLER  QVR ALEEYGCFL EYD+   
Sbjct: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60

Query: 61  ------ISAPQ---ELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQ 120
                  S+P+     YE I KGLK LFDLPLETK+KNVSDKPYHGFLD  TPFVLPLHQ
Sbjct: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120

Query: 121 SLGIENATSFQAVESFVNLFWPSGNHHF 136
           SLGIENA+S++AV+ FVNL WPSGNHHF
Sbjct: 121 SLGIENASSYEAVQFFVNLLWPSGNHHF 148

[Close]

BLAST of Lsi07G004900 vs. ExPASy TrEMBL
Match: A0A6J1K6A3 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LOC111492707 PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.6e-41
Identity = 91/135 (67.41%), Postives = 100/135 (74.07%), Query Frame = 0


Query: 1   MEIQTLAKCEVPLIKFSIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDEISAP 60
           M  ++  K EVP I FSIE    N   P+STKW+E CS VR ALEEYGCFLAE +     
Sbjct: 1   MGFRSSKKPEVPAINFSIE----NL-RPKSTKWVETCSHVRAALEEYGCFLAECEN---N 60

Query: 61  QELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAV 120
            EL +AI  GL+ LFDLPLETKMKNVSDKPYHGFLDH  PFVLPLH+S GIENA SF+ V
Sbjct: 61  VELDDAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGV 120

Query: 121 ESFVNLFWPSGNHHF 136
           ESFV L WPSGNHHF
Sbjct: 121 ESFVKLMWPSGNHHF 127

[Close]

BLAST of Lsi07G004900 vs. ExPASy TrEMBL
Match: A0A6J1DH70 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN=LOC111021051 PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 2.1e-41
Identity = 88/131 (67.18%), Postives = 100/131 (76.34%), Query Frame = 0


Query: 5   TLAKCEVPLIKFSIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELY 64
           +LAK ++P+I  SIE        P+ST WLE CS +R ALEEYGCFL E+   SA  EL 
Sbjct: 6   SLAKAKLPVINLSIE-----NVSPESTNWLEICSHLRAALEEYGCFLGEFG--SATPELD 65

Query: 65  EAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFV 124
           + I KGL+ LFDLPLETKMKNVSDKPYHGFLDH TPFVLPLHQS GIEN+ SF+AV+SFV
Sbjct: 66  DEIFKGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFV 125

Query: 125 NLFWPSGNHHF 136
           NL WPSGN  F
Sbjct: 126 NLMWPSGNRRF 129

[Close]

BLAST of Lsi07G004900 vs. NCBI nr
Match: XP_038878200.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Benincasa hispida])

HSP 1 Score: 216.1 bits (549), Expect = 1.9e-52
Identity = 108/135 (80.00%), Postives = 115/135 (85.19%), Query Frame = 0


Query: 1   MEIQTLAKCEVPLIKFSIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDEISAP 60
           M IQ+ AK EVP+IKFSIE ND +   PQSTKWLE CSQVR ALEEYGCFLAEY+  SA 
Sbjct: 1   MGIQSSAKSEVPVIKFSIE-NDEDL-HPQSTKWLETCSQVRAALEEYGCFLAEYE--SAS 60

Query: 61  QELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAV 120
            +LY+ I KGLK LFDLPLETKMKNVSDKPYHGFLDHT PFVLPLHQSLGIENATSFQAV
Sbjct: 61  SKLYDEIFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAV 120

Query: 121 ESFVNLFWPSGNHHF 136
           ESF+ L WPSGNHHF
Sbjct: 121 ESFLKLLWPSGNHHF 131

[Close]

BLAST of Lsi07G004900 vs. NCBI nr
Match: KAA0050759.1 (2-oxoglutarate-dependent dioxygenase AOP2-like [Cucumis melo var. makuwa] >TYK20444.1 2-oxoglutarate-dependent dioxygenase AOP2-like [Cucumis melo var. makuwa])

HSP 1 Score: 188.7 bits (478), Expect = 3.2e-44
Identity = 96/148 (64.86%), Postives = 108/148 (72.97%), Query Frame = 0


Query: 1   MEIQTLAKCEVPLIKF-SIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDE--- 60
           M I+ LAK EVP+IK  +I+  D + D   S  WLER  QVR ALEEYGCFL EYD+   
Sbjct: 1   MGIECLAKLEVPIIKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDES 60

Query: 61  -----ISAPQEL---YEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQS 120
                 S+  +L   YE I KGLK LFD PLETKMKNVSDKPYHGFLD   PFVLPLHQS
Sbjct: 61  SRCCNSSSSSKLDSNYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQS 120

Query: 121 LGIENATSFQAVESFVNLFWPSGNHHFW 137
           LGIENA+S++AV+SFVNL WPSGNHHFW
Sbjct: 121 LGIENASSYEAVQSFVNLLWPSGNHHFW 148

[Close]

BLAST of Lsi07G004900 vs. NCBI nr
Match: XP_022948698.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 184.1 bits (466), Expect = 8.0e-43
Identity = 90/136 (66.18%), Postives = 104/136 (76.47%), Query Frame = 0


Query: 1   MEIQTLAKCEVPLIKFSIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYD-EISA 60
           M  ++  K EVP+I FSIE    N   P+STKW+E C+ VR ALEEYGCFLAEY+ ++  
Sbjct: 1   MGFRSSTKPEVPVINFSIE----NL-GPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQ 60

Query: 61  PQELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQA 120
             EL +AI  GL+ LFDLPL+TKMKNVSDKPYHGFLDH  PFVLPLH+S GIENA SF+ 
Sbjct: 61  EVELDDAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEG 120

Query: 121 VESFVNLFWPSGNHHF 136
           VESFV L WPSGNHHF
Sbjct: 121 VESFVKLMWPSGNHHF 131

[Close]

BLAST of Lsi07G004900 vs. NCBI nr
Match: XP_023525673.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 183.7 bits (465), Expect = 1.0e-42
Identity = 90/127 (70.87%), Postives = 100/127 (78.74%), Query Frame = 0


Query: 10  EVPLIKFSIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYD-EISAPQELYEAIL 69
           EVP+I FSIE    N   P+STKW+E CS VR ALEEYGCFLAEY+ ++    EL +AI 
Sbjct: 10  EVPVISFSIE----NL-RPKSTKWVETCSHVRAALEEYGCFLAEYENDVEQEVELDDAIF 69

Query: 70  KGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFW 129
            GL+ LFDLPLETKMKNVSDKPYHGFLDH  PFVLPLH+S GIENA SF+ VESFV L W
Sbjct: 70  NGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLMW 129

Query: 130 PSGNHHF 136
           PSGNHHF
Sbjct: 130 PSGNHHF 131

[Close]

BLAST of Lsi07G004900 vs. NCBI nr
Match: KAG6606775.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 183.7 bits (465), Expect = 1.0e-42
Identity = 90/136 (66.18%), Postives = 104/136 (76.47%), Query Frame = 0


Query: 1   MEIQTLAKCEVPLIKFSIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYD-EISA 60
           M  ++  K EVP+I FSIE    N   P+STKW+E C+ VR ALEEYGCFLAEY+ ++  
Sbjct: 1   MGFRSSTKPEVPVINFSIE----NL-RPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQ 60

Query: 61  PQELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQA 120
             EL +AI  GL+ LFDLPL+TKMKNVSDKPYHGFLDH  PFVLPLH+S GIENA SF+ 
Sbjct: 61  EVELDDAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEG 120

Query: 121 VESFVNLFWPSGNHHF 136
           VESFV L WPSGNHHF
Sbjct: 121 VESFVKLMWPSGNHHF 131

[Close]

BLAST of Lsi07G004900 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 105.1 bits (261), Expect = 4.4e-23
Identity = 52/116 (44.83%), Postives = 70/116 (60.34%), Query Frame = 0


Query: 22  DYNFDD--PQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILKGLKVLFDLPL 81
           D++ +D  P +  W      +R ALEEYGCF+ +  +   P +L + +   L  LFDLP 
Sbjct: 9   DFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKD-KTPLDLLDRVFGSLVDLFDLPT 68

Query: 82  ETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWPSGNHHF 136
           +TKMKN  DKP +G++       LPLH+SLGI+NATS +A  SF  L WP GN HF
Sbjct: 69  QTKMKNKYDKPLNGYVGQIP--ALPLHESLGIDNATSLEATRSFTGLMWPQGNEHF 121

[Close]

BLAST of Lsi07G004900 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 101.7 bits (252), Expect = 4.9e-22
Identity = 47/108 (43.52%), Postives = 68/108 (62.96%), Query Frame = 0


Query: 28  PQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILKGLKVLFDLPLETKMKNVS 87
           P ++ W     +   ALEEYGCFLA YD ++  Q+L ++I    + LFDLP ETK KNV+
Sbjct: 23  PNTSTWRSVSREACEALEEYGCFLAVYDGVT--QQLDDSIFAAAEELFDLPTETKKKNVN 82

Query: 88  DKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWPSGNHHF 136
           +KPYHG++      V+PLH+ LG++  T+ +  + F +L WP GN  F
Sbjct: 83  EKPYHGYVGQMP--VIPLHEGLGVDYVTNKEIAQRFTHLMWPQGNDRF 126

[Close]

BLAST of Lsi07G004900 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 93.2 bits (230), Expect = 1.7e-19
Identity = 46/120 (38.33%), Postives = 72/120 (60.00%), Query Frame = 0


Query: 12  PLIKFSIELNDYNFDD--PQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILK 71
           P + F + + D++  +  P S+KW E  + V  ALE+YGCF A +D++S   EL  ++ +
Sbjct: 8   PSVSFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSV--ELNRSVFE 67

Query: 72  GLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWP 130
            ++ LF+LP+ TK +NVS KP+HG+L H       L++SLGI +A   + V  F    WP
Sbjct: 68  AMEDLFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWP 119

[Close]

BLAST of Lsi07G004900 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 6.6e-19
Identity = 46/128 (35.94%), Postives = 79/128 (61.72%), Query Frame = 0


Query: 10  EVPLIKFSIELNDYNFDD--PQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAI 69
           E PL+   + + D++  +  P   +W    + V+ AL++YG F A +D I  P EL +++
Sbjct: 4   ETPLLPLRLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRI--PFELRKSV 63

Query: 70  LKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLF 129
              L+ LFDLPL+TK++NVS KP+HG++      ++PL++S+GI+++   + V++F    
Sbjct: 64  FGALEELFDLPLQTKLRNVSKKPFHGYVGQYP--MVPLYESMGIDDSDIAEKVDAFTEKL 123

Query: 130 WPSGNHHF 136
           WP GN  F
Sbjct: 124 WPQGNISF 127

[Close]

BLAST of Lsi07G004900 vs. TAIR 10
Match: AT1G52810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 3.0e-16
Identity = 47/136 (34.56%), Postives = 77/136 (56.62%), Query Frame = 0


Query: 1   MEIQTLAKCEVPLIKF-SIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDEISA 60
           + ++T    ++P+I F S +L       P + +W    + VR ALEEYGCF A +D++  
Sbjct: 2   VSLETTLALQLPVIDFTSRDLK------PGTIQWDSVRADVRRALEEYGCFEALFDKV-- 61

Query: 61  PQELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQA 120
           P EL +A+    + +F LPLETK + VS + Y G++       LPL + +G++ A +   
Sbjct: 62  PLELRKAVFDASEEVFQLPLETKKRVVSKRKYRGYVGQIP--TLPLFEVMGVDFAENPDK 121

Query: 121 VESFVNLFWPSGNHHF 136
           V +F +  WP GN++F
Sbjct: 122 VNAFTHKLWPQGNNNF 127

The following BLAST results are available for this feature:

BLAST of Lsi07G004900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B5	2.6e-20	47.17	2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]
Q9ZTA2	4.4e-20	46.23	Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thali...	[more]
Q944Z9	9.9e-20	45.28	2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]
Q9ZTA3	2.4e-18	38.33	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q945B6	5.4e-18	38.33	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]

BLAST of Lsi07G004900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DAA8	1.6e-44	64.86	2-oxoglutarate-dependent dioxygenase AOP2-like OS=Cucumis melo var. makuwa OX=11...	[more]
A0A6J1GA17	3.9e-43	66.18	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A0A0LBU1	1.5e-42	64.86	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A6J1K6A3	1.6e-41	67.41	2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1DH70	2.1e-41	67.18	2-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN...	[more]

BLAST of Lsi07G004900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878200.1	1.9e-52	80.00	2-oxoglutarate-dependent dioxygenase AOP3-like [Benincasa hispida]	[more]
KAA0050759.1	3.2e-44	64.86	2-oxoglutarate-dependent dioxygenase AOP2-like [Cucumis melo var. makuwa] >TYK20...	[more]
XP_022948698.1	8.0e-43	66.18	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata]	[more]
XP_023525673.1	1.0e-42	70.87	2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo]	[more]
KAG6606775.1	1.0e-42	66.18	putative 2-oxoglutarate-dependent dioxygenase AOP1, partial [Cucurbita argyrospe...	[more]

BLAST of Lsi07G004900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52790.1	4.4e-23	44.83	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	4.9e-22	43.52	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	1.7e-19	38.33	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	6.6e-19	35.94	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52810.1	3.0e-16	34.56	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 12..94 e-value: 1.1E-7 score: 32.6
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..135 e-value: 2.0E-9 score: 39.1
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 9..135
None	No IPR available	PANTHER	PTHR47990:SF153	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 9..135
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 24..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi07G004900.1	Lsi07G004900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity