Lsi07G004470 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi07G004470
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase
Location	chr07: 4763841 .. 4765670 (-)
RNA-Seq Expression	Lsi07G004470
Synteny	Lsi07G004470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTAATAAATATTTTTATCTACCACCCTAGGTTTGTTTTAATAATTTTCTTTTCCTTTTAGTTCATCAATTAAATTTTACATCTACCACGTCCCCCATAAATAAATGTCGTTCTATGTAAGAAACTATAATTCTCAAGCTTGATATTCGATATAAATACATATGCCAGAGAGAGTTAGGTAATGAAAGCTTCAAAAAAATTGGCCATGGACATGCAAACTCAAGAACCAAAGAAAATGGTGAAATCATTTCCTTTTCCCTTTTCTTTGTTCATATCGTTTGTGTTTTTGCACCATTTCATTTTAATTGGCGTGCATTTTGGGTTGCTTTTGCATTATATGTTATTACTGGTTTAAGCATCAGTATTTCATATCATAGAAATCTTGCACATAAGAGTTTCAAGCTTCCTAAACCGCTTGAATACTTTTTTGCTTATTGTGCAGCTCATGCACTTCAGGTTTGATAAATAAATTCCAAAATTTCTTTTTTTCTCTATTTTTCTATTTTATCTACTTAATTATTTTTCTATTTTCTTTAGGGTGATCCAATCGATTGGGTGAGTACACATAGATGTCATCATCAATTTGTCGATACAAAAAGAGATCCACATAGTCCTATGGAAGGATTTTGGTTTAGTCATATTACTTGGCTTTTCGATTCGTATAATTTGACCAAAAAAGTTTGTCCAAAATATTTTACCAATTTTCAAAAGATCGAAAGAAGCATATTTATATGGTTCTCGAAGCATGGGAGACCCGACAACGTTCGTGATTTGGAGAAGCAAACCTTCTATAGGTTTATCCATAAAACATATTTTCTTCATCACATTGCTCTTGCGATCCTACTCTATGAAGTTGGGGACTTCCATTCCTTATATGGGGCACGGTAAAGTTTCATATATATATATATATATATATTATTTGGCGTCCCAACTATTTACTTTTTAAATTAAATTCATATACATTTCATGTATTCCTTTCAATAAATAACAAGTACTACTCATTTCAACAATATGTAATATGATTTATTACGAAAAGACCAATGATATGATTGTAATGCCTCAAGCCCAACAGGATTTGGAATTTTGATTTAGTATCTAACAACCTTGACATTCTCTTACATCTCTAGTAGGATGTGGTTCAGCGACGAACCAATGTGAAAGTTGAGGGAAAATGTGACTTCAAGTAGAGTAGATTATTACCTATATTAACAATGTGCATCTTCATTTTCAGTGGCGTAATTATAAGAAGTTCAAAGTTGATTGTGTTTAACTAGGAACAATCCTATGTTGAGTGATTTTCAGTTTTTTTTAAAAGCATGTTAGTGAGGACATAACATGCTAAAAGGACTTATGTTAGTTTATAGAAATGAGGCTTCATGCTTACTCACGATTCAAGAAAGTACGAATGATGTTGCTAGGGTTTGCAAGGGATGTAAGAATTGTCAGGGACCATCAGGTACCAAATTAGAATTTTGAATCTTGGTCACAATTTTTAGACTTGGGACATTACCAAGATTTTTTTAGAACAAGGGTTAGTAGGTGCATCCGAACATCTCAACTAGATTGACACGTTCTTATCACCCTCATTATATTCCACGATCTAAAATATATTCAATTATTTCTTAACAAATCATGGTAAAAAGAACATATAATTCTCCTCCAATCTTGCATTTTGATAGATGTATATAAGATTTTCATTGTGTACTTAGAAATATTAACACGTTAGCTTCAAATTATATAGTGTGTGAGGATCGTAGTGCTCTTGCACATAACATTTATGGTGAACTCGGTATGTCATATATGGGGAAAACGACGATGGAATACTAG

mRNA sequence

ATGGGTAATGAAAGCTTCAAAAAAATTGGCCATGGACATGCAAACTCAAGAACCAAAGAAAATGGTGAAATCATTTCCTTTTCCCTTTTCTTTGTTCATATCGTTTGTGTTTTTGCACCATTTCATTTTAATTGGCGTGCATTTTGGGTTGCTTTTGCATTATATGTTATTACTGGTTTAAGCATCAGTATTTCATATCATAGAAATCTTGCACATAAGAGTTTCAAGCTTCCTAAACCGCTTGAATACTTTTTTGCTTATTGTGCAGCTCATGCACTTCAGGGTGATCCAATCGATTGGGTGAGTACACATAGATGTCATCATCAATTTGTCGATACAAAAAGAGATCCACATAGTCCTATGGAAGGATTTTGGTTTAGTCATATTACTTGGCTTTTCGATTCGTATAATTTGACCAAAAAAGTTTGTCCAAAATATTTTACCAATTTTCAAAAGATCGAAAGAAGCATATTTATATGGTTCTCGAAGCATGGGAGACCCGACAACGTTCGTGATTTGGAGAAGCAAACCTTCTATAGGTTTATCCATAAAACATATTTTCTTCATCACATTGCTCTTGCGATCCTACTCTATGAAGTTGGGGACTTCCATTCCTTATATGGGGCACGAAATATTAACACGTTAGCTTCAAATTATATAGTGTGTGAGGATCGTAGTGCTCTTGCACATAACATTTATGGTGAACTCGGTATGTCATATATGGGGAAAACGACGATGGAATACTAG

Coding sequence (CDS)

ATGGGTAATGAAAGCTTCAAAAAAATTGGCCATGGACATGCAAACTCAAGAACCAAAGAAAATGGTGAAATCATTTCCTTTTCCCTTTTCTTTGTTCATATCGTTTGTGTTTTTGCACCATTTCATTTTAATTGGCGTGCATTTTGGGTTGCTTTTGCATTATATGTTATTACTGGTTTAAGCATCAGTATTTCATATCATAGAAATCTTGCACATAAGAGTTTCAAGCTTCCTAAACCGCTTGAATACTTTTTTGCTTATTGTGCAGCTCATGCACTTCAGGGTGATCCAATCGATTGGGTGAGTACACATAGATGTCATCATCAATTTGTCGATACAAAAAGAGATCCACATAGTCCTATGGAAGGATTTTGGTTTAGTCATATTACTTGGCTTTTCGATTCGTATAATTTGACCAAAAAAGTTTGTCCAAAATATTTTACCAATTTTCAAAAGATCGAAAGAAGCATATTTATATGGTTCTCGAAGCATGGGAGACCCGACAACGTTCGTGATTTGGAGAAGCAAACCTTCTATAGGTTTATCCATAAAACATATTTTCTTCATCACATTGCTCTTGCGATCCTACTCTATGAAGTTGGGGACTTCCATTCCTTATATGGGGCACGAAATATTAACACGTTAGCTTCAAATTATATAGTGTGTGAGGATCGTAGTGCTCTTGCACATAACATTTATGGTGAACTCGGTATGTCATATATGGGGAAAACGACGATGGAATACTAG

Protein sequence

MGNESFKKIGHGHANSRTKENGEIISFSLFFVHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPLEYFFAYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKVCPKYFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVGDFHSLYGARNINTLASNYIVCEDRSALAHNIYGELGMSYMGKTTMEY

Homology

[Close]

BLAST of Lsi07G004470 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 208.0 bits (528), Expect = 1.2e-52
Identity = 96/173 (55.49%), Postives = 122/173 (70.52%), Query Frame = 0


Query: 32  VHIVCVFAPFHFNWRAFWVAFALYVITG-LSISISYHRNLAHKSFKLPKPLEYFFAYCAA 91
           +H++ + APF FNWRA  VAF LY++TG L I++S+HRNL+HK+FKLPK LEY FAYC A
Sbjct: 112 MHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGA 171

Query: 92  HALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKVCPKYFTNF 151
            ALQG+PIDWVSTHR HHQF D+ RDPHSP++GFWFSH+ W+FD+  +T++         
Sbjct: 172 QALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFDTNTITQRC-------- 231

Query: 152 QKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVGDF 204
                         G P+NV DLEKQ FYRF+  TY LH +ALA+ LY +G F
Sbjct: 232 --------------GEPNNVGDLEKQPFYRFLRTTYILHPLALAVALYAMGGF 262

[Close]

BLAST of Lsi07G004470 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.7e-49
Identity = 93/197 (47.21%), Postives = 119/197 (60.41%), Query Frame = 0


Query: 26  SFSLFFVHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPLEYFF 85
           +F+  FVH +C+ APF+F W A  VA  +Y + GL I++SYHRNLAH+SFK+PK LEYFF
Sbjct: 42  AFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFF 101

Query: 86  AYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKVCPK 145
           AYC   A+QGDPIDWVSTHR HHQF D+ RDPHSP EGFWFSH+ WLFD+  L +K    
Sbjct: 102 AYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC--- 161

Query: 146 YFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVGDFHS 205
                              GR  NV DL++Q +Y+F+ +T   H +    LLY  G    
Sbjct: 162 -------------------GRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSF 216

Query: 206 LYGARNINTLASNYIVC 223
           L     I     +++ C
Sbjct: 222 LTWGMGIGVAMEHHVTC 216

[Close]

BLAST of Lsi07G004470 vs. ExPASy Swiss-Prot
Match: Q9SID2 (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=2)

HSP 1 Score: 194.9 bits (494), Expect = 1.1e-48
Identity = 94/201 (46.77%), Postives = 119/201 (59.20%), Query Frame = 0


Query: 23  EIISFSL-FFVHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPL 82
           + + FS  F VH + + APF+F W A WV F  Y I GL I++SYHRNLAH+SFK+PK L
Sbjct: 40  DYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLAHRSFKVPKWL 99

Query: 83  EYFFAYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKK 142
           EY  AYCA  A+QGDPIDWVSTHR HHQF D++RDPHSP EGFWFSH+ W++DS  L   
Sbjct: 100 EYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWIYDSAYLV-- 159

Query: 143 VCPKYFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVG 202
                               SK GR  NV DL++Q FYRF+ KT   H + L   L+ +G
Sbjct: 160 --------------------SKCGRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLG 218

Query: 203 DFHSLYGARNINTLASNYIVC 223
               +     +      ++ C
Sbjct: 220 GMSFVTWGMGVGAALEVHVTC 218

[Close]

BLAST of Lsi07G004470 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.1e-45
Identity = 93/193 (48.19%), Postives = 119/193 (61.66%), Query Frame = 0


Query: 27  FSLFFV---HIVCVFAPFHFNWRAFWVAFALYVITGL-SISISYHRNLAHKSFKLPKPLE 86
           F + F+   H++C+FAP  F+W++FWV FALY I G+   ++S+HRNL H+SFKLPK LE
Sbjct: 46  FHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRSFKLPKYLE 105

Query: 87  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKV 146
           YFFAY   HALQGDP+ WVSTHR HH++ DT  DPHSP+EGFWF HI WLFDS       
Sbjct: 106 YFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFDS------- 165

Query: 147 CPKYFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVGD 206
             KY                K GR +N  DL KQ++YRF+ +T+  H    A LLY  G 
Sbjct: 166 --KYI-------------IEKCGRYENAGDLMKQSYYRFLERTFVYHVYLQAALLYLFGG 216

Query: 207 FHSLYGARNINTL 216
           F  +     + T+
Sbjct: 226 FPFIVWGMAVRTI 216

[Close]

BLAST of Lsi07G004470 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 3.3e-45
Identity = 90/185 (48.65%), Postives = 115/185 (62.16%), Query Frame = 0


Query: 32  VHIVCVFAPFHFNWRAFWVAFALYVITGL-SISISYHRNLAHKSFKLPKPLEYFFAYCAA 91
           +H++C+ APF F+W AFW++  LY + G+   ++SYHRNL H+SFKLPK LEYFFAY   
Sbjct: 51  LHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYHRNLTHRSFKLPKYLEYFFAYVGL 110

Query: 92  HALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKVCPKYFTNF 151
           HALQGDP+ WVSTHR HH+F DT  DPHSP+EGFWFSHI WLFDS         KY    
Sbjct: 111 HALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWFSHIFWLFDS---------KYI--- 170

Query: 152 QKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVGDFHSLYGAR 211
                       + GR +N  DL KQ++YRF+ +T+  H    A LLY  G F  +    
Sbjct: 171 ----------LEECGRYENAGDLLKQSYYRFLERTFVFHVYLQAALLYMFGGFPFIVWGM 213

Query: 212 NINTL 216
            + T+
Sbjct: 231 AVRTV 213

[Close]

BLAST of Lsi07G004470 vs. ExPASy TrEMBL
Match: A0A0A0KYH4 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006130 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.7e-92
Identity = 156/179 (87.15%), Postives = 167/179 (93.30%), Query Frame = 0


Query: 23  EIISFSLFFVHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPLE 82
           +II FSLF VHI C+FAPFHF W AF VAFALY ITGL IS+SYHRNLAHKSFKLPK +E
Sbjct: 27  KIIFFSLFIVHIACIFAPFHFTWPAFCVAFALYFITGLCISVSYHRNLAHKSFKLPKSME 86

Query: 83  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKV 142
           YF AYCAAHALQGDPIDWVSTHRCHHQFVDT++DPHSP+EGFWFSHITWLFDSYNLTKKV
Sbjct: 87  YFLAYCAAHALQGDPIDWVSTHRCHHQFVDTEKDPHSPIEGFWFSHITWLFDSYNLTKKV 146

Query: 143 CPKYFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVG 202
           CP YFT+FQK++RSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHI LAILLY+VG
Sbjct: 147 CPNYFTDFQKVDRSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHILLAILLYQVG 205

[Close]

BLAST of Lsi07G004470 vs. ExPASy TrEMBL
Match: A0A1S3BYW2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103494693 PE=3 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 6.3e-92
Identity = 156/179 (87.15%), Postives = 168/179 (93.85%), Query Frame = 0


Query: 23  EIISFSLFFVHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPLE 82
           +II FSLF VHI C+FAPFHF W AF +AFALYV+TGLSIS+SYHRNLAH+SFKLPK LE
Sbjct: 18  KIIFFSLFIVHIGCIFAPFHFTWHAFCLAFALYVVTGLSISVSYHRNLAHRSFKLPKLLE 77

Query: 83  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKV 142
           YF AYCAAHALQGDPIDWVSTHRCHHQFVDT++DPHSP+ GFWFSHITWLFDSYNLTKKV
Sbjct: 78  YFLAYCAAHALQGDPIDWVSTHRCHHQFVDTEKDPHSPIRGFWFSHITWLFDSYNLTKKV 137

Query: 143 CPKYFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVG 202
           CPKYFTNFQKI+RSIFIWFSKHGRPDNVRDLEKQ FYRFIHKTYFLHHI LAILL++VG
Sbjct: 138 CPKYFTNFQKIDRSIFIWFSKHGRPDNVRDLEKQIFYRFIHKTYFLHHILLAILLFQVG 196

[Close]

BLAST of Lsi07G004470 vs. ExPASy TrEMBL
Match: A0A6J1GWI3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457789 PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 8.2e-92
Identity = 152/179 (84.92%), Postives = 168/179 (93.85%), Query Frame = 0


Query: 23  EIISFSLFFVHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPLE 82
           + IS +LF +HI+C+FAPFHFNWRAFW++FALYVITGLSIS+SYHRNLAHKSF+LPK LE
Sbjct: 24  QYISIALFILHILCIFAPFHFNWRAFWLSFALYVITGLSISVSYHRNLAHKSFELPKVLE 83

Query: 83  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKV 142
           YFFAYCAAHALQGDPIDWVSTHRCHHQFVDT RDPHSP++GFWFSHI WLFDSY LTK+V
Sbjct: 84  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTDRDPHSPIQGFWFSHILWLFDSYTLTKRV 143

Query: 143 CPKYFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVG 202
           CPKYFT+F+KIERSI IWFSKHGRPDNVRDLEKQ FYRFIHKTY LHH+A A+LLYEVG
Sbjct: 144 CPKYFTDFKKIERSIIIWFSKHGRPDNVRDLEKQAFYRFIHKTYLLHHVAFAVLLYEVG 202

[Close]

BLAST of Lsi07G004470 vs. ExPASy TrEMBL
Match: A0A6J1J0F3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111480111 PE=3 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 9.1e-91
Identity = 153/198 (77.27%), Postives = 173/198 (87.37%), Query Frame = 0


Query: 23  EIISFSLFFVHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPLE 82
           + IS +LF +H +C+FAPF+FNW AFWV+FALYVITGLSIS+SYHRNLAHKSF+LPK LE
Sbjct: 24  QYISIALFILHFLCIFAPFYFNWHAFWVSFALYVITGLSISVSYHRNLAHKSFELPKLLE 83

Query: 83  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKV 142
           YFFAYCAAHALQGDPIDWVSTHRCHHQFVDT+RDPHSP++GFWFSHI WLFDSY LTK+V
Sbjct: 84  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTERDPHSPIQGFWFSHILWLFDSYTLTKRV 143

Query: 143 CPKYFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVGD 202
           CPKYFT+F+KIERSI IWFSKHGRPDNVRDLEKQ FYRFIHKTY LHH+A A+LLYEVG 
Sbjct: 144 CPKYFTDFKKIERSIIIWFSKHGRPDNVRDLEKQAFYRFIHKTYLLHHVAFAVLLYEVGG 203

Query: 203 FHSLYGARNINTLASNYI 221
              L     + T+   +I
Sbjct: 204 LPFLIWGTCVRTVVVLHI 221

[Close]

BLAST of Lsi07G004470 vs. ExPASy TrEMBL
Match: A0A6J1DHZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021091 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 6.3e-84
Identity = 145/199 (72.86%), Postives = 168/199 (84.42%), Query Frame = 0


Query: 25  ISFSLFFVHIVCVFAPFHFNWRAFWVAFALYVITGL-SISISYHRNLAHKSFKLPKPLEY 84
           ++ SLFFVH++ +FAPF+FNWRAFW+AFALYV TGL  IS+SYHRNLAHKSF LPK LEY
Sbjct: 51  VACSLFFVHLLSMFAPFYFNWRAFWIAFALYVATGLFGISVSYHRNLAHKSFTLPKWLEY 110

Query: 85  FFAYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKVC 144
            FAYC AHALQGDPIDWV+THRCHH+FVDT+RDPHSP++GFWFSHITWL DSY+LTK+VC
Sbjct: 111 LFAYCGAHALQGDPIDWVNTHRCHHRFVDTERDPHSPIQGFWFSHITWLLDSYSLTKRVC 170

Query: 145 PKYFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVGDF 204
           PKYFT+FQK+ER+IF+ FSKHGRPDNV DLEKQTFYRFIHKTYFLH I LAI+LY  G  
Sbjct: 171 PKYFTDFQKVERNIFVMFSKHGRPDNVGDLEKQTFYRFIHKTYFLHPIILAIILYAYGGA 230

Query: 205 HSLYGARNINTLASNYIVC 223
             L     +  +   +I C
Sbjct: 231 PFLIWGMGVRIVVVLHITC 249

[Close]

BLAST of Lsi07G004470 vs. NCBI nr
Match: XP_023526679.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 350.1 bits (897), Expect = 1.5e-92
Identity = 154/179 (86.03%), Postives = 169/179 (94.41%), Query Frame = 0


Query: 23  EIISFSLFFVHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPLE 82
           + IS +LF +HI+C+FAPFHFNWRAFWV+FALYVITGLSIS+SYHRNLAHKSF+LPK LE
Sbjct: 24  QYISIALFILHILCIFAPFHFNWRAFWVSFALYVITGLSISVSYHRNLAHKSFELPKLLE 83

Query: 83  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKV 142
           YFFAYCAAHALQGDPIDWVSTHRCHHQFVDT+RDPHSP++GFWFSHI WLFDSY LTK+V
Sbjct: 84  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTERDPHSPVQGFWFSHILWLFDSYTLTKRV 143

Query: 143 CPKYFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVG 202
           CPKYFTNF+KIERSI IWFSKHGRPDNVRDLEKQ FYRFIHKTY LHH+A A+LLYEVG
Sbjct: 144 CPKYFTNFKKIERSIIIWFSKHGRPDNVRDLEKQAFYRFIHKTYLLHHVAFAVLLYEVG 202

[Close]

BLAST of Lsi07G004470 vs. NCBI nr
Match: XP_023526681.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 350.1 bits (897), Expect = 1.5e-92
Identity = 154/179 (86.03%), Postives = 169/179 (94.41%), Query Frame = 0


Query: 23  EIISFSLFFVHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPLE 82
           + IS +LF +HI+C+FAPFHFNWRAFWV+FALYVITGLSIS+SYHRNLAHKSF+LPK LE
Sbjct: 24  QYISIALFILHILCIFAPFHFNWRAFWVSFALYVITGLSISVSYHRNLAHKSFELPKLLE 83

Query: 83  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKV 142
           YFFAYCAAHALQGDPIDWVSTHRCHHQFVDT+RDPHSP++GFWFSHI WLFDSY LTK+V
Sbjct: 84  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTERDPHSPVQGFWFSHILWLFDSYTLTKRV 143

Query: 143 CPKYFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVG 202
           CPKYFTNF+KIERSI IWFSKHGRPDNVRDLEKQ FYRFIHKTY LHH+A A+LLYEVG
Sbjct: 144 CPKYFTNFKKIERSIIIWFSKHGRPDNVRDLEKQAFYRFIHKTYLLHHVAFAVLLYEVG 202

[Close]

BLAST of Lsi07G004470 vs. NCBI nr
Match: XP_023526680.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 350.1 bits (897), Expect = 1.5e-92
Identity = 154/179 (86.03%), Postives = 169/179 (94.41%), Query Frame = 0


Query: 23  EIISFSLFFVHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPLE 82
           + IS +LF +HI+C+FAPFHFNWRAFWV+FALYVITGLSIS+SYHRNLAHKSF+LPK LE
Sbjct: 24  QYISIALFILHILCIFAPFHFNWRAFWVSFALYVITGLSISVSYHRNLAHKSFELPKLLE 83

Query: 83  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKV 142
           YFFAYCAAHALQGDPIDWVSTHRCHHQFVDT+RDPHSP++GFWFSHI WLFDSY LTK+V
Sbjct: 84  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTERDPHSPVQGFWFSHILWLFDSYTLTKRV 143

Query: 143 CPKYFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVG 202
           CPKYFTNF+KIERSI IWFSKHGRPDNVRDLEKQ FYRFIHKTY LHH+A A+LLYEVG
Sbjct: 144 CPKYFTNFKKIERSIIIWFSKHGRPDNVRDLEKQAFYRFIHKTYLLHHVAFAVLLYEVG 202

[Close]

BLAST of Lsi07G004470 vs. NCBI nr
Match: XP_023526682.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 350.1 bits (897), Expect = 1.5e-92
Identity = 154/179 (86.03%), Postives = 169/179 (94.41%), Query Frame = 0


Query: 23  EIISFSLFFVHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPLE 82
           + IS +LF +HI+C+FAPFHFNWRAFWV+FALYVITGLSIS+SYHRNLAHKSF+LPK LE
Sbjct: 24  QYISIALFILHILCIFAPFHFNWRAFWVSFALYVITGLSISVSYHRNLAHKSFELPKLLE 83

Query: 83  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKV 142
           YFFAYCAAHALQGDPIDWVSTHRCHHQFVDT+RDPHSP++GFWFSHI WLFDSY LTK+V
Sbjct: 84  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTERDPHSPVQGFWFSHILWLFDSYTLTKRV 143

Query: 143 CPKYFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVG 202
           CPKYFTNF+KIERSI IWFSKHGRPDNVRDLEKQ FYRFIHKTY LHH+A A+LLYEVG
Sbjct: 144 CPKYFTNFKKIERSIIIWFSKHGRPDNVRDLEKQAFYRFIHKTYLLHHVAFAVLLYEVG 202

[Close]

BLAST of Lsi07G004470 vs. NCBI nr
Match: KAG6581940.1 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 348.2 bits (892), Expect = 5.8e-92
Identity = 153/179 (85.47%), Postives = 168/179 (93.85%), Query Frame = 0


Query: 23  EIISFSLFFVHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPLE 82
           + IS +LF +HI+C+FAPFHFNWRAFWV+FALYVITGLSIS+SYHRNLAHKSF+LPK LE
Sbjct: 24  QYISIALFILHILCIFAPFHFNWRAFWVSFALYVITGLSISVSYHRNLAHKSFELPKVLE 83

Query: 83  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKV 142
           YFFAYCAAHALQGDPIDWVSTHRCHHQFVDT RDPHSP++GFWFSHI WLFDSY LTK+V
Sbjct: 84  YFFAYCAAHALQGDPIDWVSTHRCHHQFVDTNRDPHSPIQGFWFSHILWLFDSYTLTKRV 143

Query: 143 CPKYFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVG 202
           CPKYFT+F+KIERSI IWFSKHGRPDNVRDLEKQ FYRFIHKTY LHH+A A+LLYEVG
Sbjct: 144 CPKYFTDFKKIERSIIIWFSKHGRPDNVRDLEKQAFYRFIHKTYLLHHVAFAVLLYEVG 202

[Close]

BLAST of Lsi07G004470 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 208.0 bits (528), Expect = 8.8e-54
Identity = 96/173 (55.49%), Postives = 122/173 (70.52%), Query Frame = 0


Query: 32  VHIVCVFAPFHFNWRAFWVAFALYVITG-LSISISYHRNLAHKSFKLPKPLEYFFAYCAA 91
           +H++ + APF FNWRA  VAF LY++TG L I++S+HRNL+HK+FKLPK LEY FAYC A
Sbjct: 112 MHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGA 171

Query: 92  HALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKVCPKYFTNF 151
            ALQG+PIDWVSTHR HHQF D+ RDPHSP++GFWFSH+ W+FD+  +T++         
Sbjct: 172 QALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFDTNTITQRC-------- 231

Query: 152 QKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVGDF 204
                         G P+NV DLEKQ FYRF+  TY LH +ALA+ LY +G F
Sbjct: 232 --------------GEPNNVGDLEKQPFYRFLRTTYILHPLALAVALYAMGGF 262

[Close]

BLAST of Lsi07G004470 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 197.6 bits (501), Expect = 1.2e-50
Identity = 93/197 (47.21%), Postives = 119/197 (60.41%), Query Frame = 0


Query: 26  SFSLFFVHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPLEYFF 85
           +F+  FVH +C+ APF+F W A  VA  +Y + GL I++SYHRNLAH+SFK+PK LEYFF
Sbjct: 42  AFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFF 101

Query: 86  AYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKVCPK 145
           AYC   A+QGDPIDWVSTHR HHQF D+ RDPHSP EGFWFSH+ WLFD+  L +K    
Sbjct: 102 AYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC--- 161

Query: 146 YFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVGDFHS 205
                              GR  NV DL++Q +Y+F+ +T   H +    LLY  G    
Sbjct: 162 -------------------GRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSF 216

Query: 206 LYGARNINTLASNYIVC 223
           L     I     +++ C
Sbjct: 222 LTWGMGIGVAMEHHVTC 216

[Close]

BLAST of Lsi07G004470 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 194.9 bits (494), Expect = 7.7e-50
Identity = 94/201 (46.77%), Postives = 119/201 (59.20%), Query Frame = 0


Query: 23  EIISFSL-FFVHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPL 82
           + + FS  F VH + + APF+F W A WV F  Y I GL I++SYHRNLAH+SFK+PK L
Sbjct: 40  DYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLAHRSFKVPKWL 99

Query: 83  EYFFAYCAAHALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKK 142
           EY  AYCA  A+QGDPIDWVSTHR HHQF D++RDPHSP EGFWFSH+ W++DS  L   
Sbjct: 100 EYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWIYDSAYLV-- 159

Query: 143 VCPKYFTNFQKIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLYEVG 202
                               SK GR  NV DL++Q FYRF+ KT   H + L   L+ +G
Sbjct: 160 --------------------SKCGRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLG 218

Query: 203 DFHSLYGARNINTLASNYIVC 223
               +     +      ++ C
Sbjct: 220 GMSFVTWGMGVGAALEVHVTC 218

[Close]

BLAST of Lsi07G004470 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 177.9 bits (450), Expect = 9.7e-45
Identity = 85/167 (50.90%), Postives = 108/167 (64.67%), Query Frame = 0


Query: 32  VHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPLEYFFAYCAAH 91
           VH++C+ APF++ W AF     L ++T L I+ SYHRNL H+SFKLPK LEY FAY A  
Sbjct: 42  VHLLCLLAPFNYKWEAFRFGIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALL 101

Query: 92  ALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKVCPKYFTNFQ 151
           ALQGDP+DWVS HR HHQF D+ RDPHSP+EGFWFSH+ W+FD+  + +K          
Sbjct: 102 ALQGDPLDWVSIHRFHHQFTDSDRDPHSPIEGFWFSHVLWIFDTDYIREKC--------- 161

Query: 152 KIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLY 199
                        GR +NV DL++Q FYRF+ KT  LH +A   L+Y
Sbjct: 162 -------------GRRNNVMDLKQQWFYRFLKKTLVLHILAFWTLIY 186

[Close]

BLAST of Lsi07G004470 vs. TAIR 10
Match: AT1G06120.1 (Fatty acid desaturase family protein )

HSP 1 Score: 175.6 bits (444), Expect = 4.8e-44
Identity = 86/167 (51.50%), Postives = 107/167 (64.07%), Query Frame = 0


Query: 32  VHIVCVFAPFHFNWRAFWVAFALYVITGLSISISYHRNLAHKSFKLPKPLEYFFAYCAAH 91
           VH++C+ APF++ W AF  A  + + T LSI+ SYHRNL H+SFKLPK LEY FAY A  
Sbjct: 42  VHLLCLLAPFNYTWEAFRFAAMVGISTNLSITFSYHRNLTHRSFKLPKWLEYPFAYSALF 101

Query: 92  ALQGDPIDWVSTHRCHHQFVDTKRDPHSPMEGFWFSHITWLFDSYNLTKKVCPKYFTNFQ 151
           ALQG PIDWVSTHR HHQF D+ RDPHSP+EGFWFSH+ W+FD+  + +K          
Sbjct: 102 ALQGHPIDWVSTHRFHHQFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKC--------- 161

Query: 152 KIERSIFIWFSKHGRPDNVRDLEKQTFYRFIHKTYFLHHIALAILLY 199
                        G  DNV DL++Q FYRF+  T  LH +   IL+Y
Sbjct: 162 -------------GGRDNVMDLKQQWFYRFLQNTIGLHILTFWILVY 186

The following BLAST results are available for this feature:

BLAST of Lsi07G004470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949X0	1.2e-52	55.49	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab...	[more]
O65797	1.7e-49	47.21	Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=...	[more]
Q9SID2	1.1e-48	46.77	Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=...	[more]
P0DOW2	1.1e-45	48.19	Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1	[more]
P0DOW3	3.3e-45	48.65	Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1	[more]

BLAST of Lsi07G004470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KYH4	3.7e-92	87.15	FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G0061...	[more]
A0A1S3BYW2	6.3e-92	87.15	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1GWI3	8.2e-92	84.92	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1J0F3	9.1e-91	77.27	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1DHZ7	6.3e-84	72.86	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]

BLAST of Lsi07G004470 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023526679.1	1.5e-92	86.03	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like is...	[more]
XP_023526681.1	1.5e-92	86.03	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like is...	[more]
XP_023526680.1	1.5e-92	86.03	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like is...	[more]
XP_023526682.1	1.5e-92	86.03	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like is...	[more]
KAG6581940.1	5.8e-92	85.47	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partia...	[more]

BLAST of Lsi07G004470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15850.1	8.8e-54	55.49	fatty acid desaturase 5	[more]
AT1G06080.1	1.2e-50	47.21	delta 9 desaturase 1	[more]
AT2G31360.1	7.7e-50	46.77	16:0delta9 desaturase 2	[more]
AT1G06100.1	9.7e-45	50.90	Fatty acid desaturase family protein	[more]
AT1G06120.1	4.8e-44	51.50	Fatty acid desaturase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015876	Acyl-CoA desaturase	PRINTS	PR00075	FACDDSATRASE	coord: 67..87 score: 37.59 coord: 104..133 score: 51.75 coord: 44..66 score: 23.8
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 163..205
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 32..161
IPR015876	Acyl-CoA desaturase	CDD	cd03505	Delta9-FADS-like	coord: 44..132 e-value: 1.29991E-36 score: 125.746
None	No IPR available	PANTHER	PTHR11351:SF94	BNAC05G37460D PROTEIN	coord: 163..205 coord: 32..161

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi07G004470.1	Lsi07G004470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009979	16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water