Lsi07G004320 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G004320
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPlant protein of unknown function (DUF247)
Locationchr07: 4607341 .. 4608966 (-)
RNA-Seq ExpressionLsi07G004320
SyntenyLsi07G004320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATCCAATTCTTCGTCTTCGACCTTCGACGAGCTCAAATGGGTCATTCAAATTCGTCGGACCCTCGAAGAAGAACTCGACGACGACGACGGGGGGATTCCAGTAACAATCTTCAAGGTCCCTAAATTGTTAATGGCGTCCGATCCACATTCCTACACTCCACAATTGGTTGCGTTGGGTCCTTACCATTGTTGGCGTCCGGAGCTCCGTGAGATGGACAGATTCAAGCTCTCTTCTGCTAAAAGGGCTAAGAAATTCAGCCCTATAAACCTCCAATTTCACGATCTCGTTGAACAATTGTCGAAGCTCCAGCAGAAGATTCGAGCGTGTTACCACGGTTACTTGGATCTTAATGCCGAAACTTTAGCCTGGATGATGGCCGTTGATGCCTCTTTCTTGCTTGAGTTTCTCCATGTCTACGCGGGAGAATTGAAGAGTCTCCGGCGAGTTTCTTCTAGAATGTCGCATTTGATGGATTACGCCGGGACGAAGCCGGCACATAACGCTGTTCTCAGAGATATAATCATGCTCGAAAATCAAATTCCATTGTTCGTATTGAGGAAGATGATGGAATTCCAATTCGAATCCTCTGAACAATCTGATGAGATTTTGCTCTCCATGATGAACGGTTTCTTCACAGAGCTTTCTCCATTCAACACCACGGACGGGATTCCAGATATTCAAATTTCAGATTGCGCTCATTTACTGGACTTTTTATACCAAATGATCACTCCTAAATCGAAAGAACCATCAGAAATTATCGAAATCCCTAATGAAATCGAACAATCTCCCCAAATTTCGAGTTACAGAAAACGATTCGCACACATATCCAAGCAATTCCTCAAACACCTAATTAGTTTCACAAAGCGAGCGATGCAATCTCAACATGTGAAGACGATCGCGCGGCTGCCATGGACGATTCTCTCCACGATCCCTCCATTCGCAGTGTTAAAACAGATTCTATTCTTCAGAGAGCAACAAGAAGCCAAGGAAGAAACAGAATCTCAAAACCCTAATTCAAATCCAAACGCAAATAGACCGCCATTAACAGAGGAAATAACAATACCTTCCGTTTCGGAGCTCTTCAAAGCCGGAATCCGCTTCTCACCCTCCCATGGCGGAATCTCTACAATCACCTTCAACGCCAAAACCGGAATGCTGTACCTTCCAACGGCGAGACTCGACGTGAACACACAAGCAACGCTGAGAAACCTCGTAGCATACGAAGCGTCAAGCGGATTAGGGGCGCTGGTTTTCACAAGATACACGGAATTGATGAACGGAATCGTGGATTCCAACGAGGATTTGAGATTGCTAAGAGAAGGAGGGGTGATTGTGAACCATCTGAAGAGCGACGAAGAGGGTGCTGCGATGTGGAATGGGATGAGCAAATCGATCAGATTGAGCAGAGTATTGGAGTTGGACAAGGTGATTGAAGAGGTGAATGAGTATTACAATGGATTATGGAGAGTGAAAATGGGAAGATTTGTGAAGGAATATGTTTACAGTTCTTGGCCTTTGCTTGCTTTAATGGCGGTCGCTTGGTTTCTGTTCTTGATCGCTCTCCAAGCTTTTTGCTCTCTGTTTGGTTGCTTTCGCGCCCTTCCTCGCAAGGGTTCTTGA

mRNA sequence

ATGGCATCCAATTCTTCGTCTTCGACCTTCGACGAGCTCAAATGGGTCATTCAAATTCGTCGGACCCTCGAAGAAGAACTCGACGACGACGACGGGGGGATTCCAGTAACAATCTTCAAGGTCCCTAAATTGTTAATGGCGTCCGATCCACATTCCTACACTCCACAATTGGTTGCGTTGGGTCCTTACCATTGTTGGCGTCCGGAGCTCCGTGAGATGGACAGATTCAAGCTCTCTTCTGCTAAAAGGGCTAAGAAATTCAGCCCTATAAACCTCCAATTTCACGATCTCGTTGAACAATTGTCGAAGCTCCAGCAGAAGATTCGAGCGTGTTACCACGGTTACTTGGATCTTAATGCCGAAACTTTAGCCTGGATGATGGCCGTTGATGCCTCTTTCTTGCTTGAGTTTCTCCATGTCTACGCGGGAGAATTGAAGAGTCTCCGGCGAGTTTCTTCTAGAATGTCGCATTTGATGGATTACGCCGGGACGAAGCCGGCACATAACGCTGTTCTCAGAGATATAATCATGCTCGAAAATCAAATTCCATTGTTCGTATTGAGGAAGATGATGGAATTCCAATTCGAATCCTCTGAACAATCTGATGAGATTTTGCTCTCCATGATGAACGGTTTCTTCACAGAGCTTTCTCCATTCAACACCACGGACGGGATTCCAGATATTCAAATTTCAGATTGCGCTCATTTACTGGACTTTTTATACCAAATGATCACTCCTAAATCGAAAGAACCATCAGAAATTATCGAAATCCCTAATGAAATCGAACAATCTCCCCAAATTTCGAGTTACAGAAAACGATTCGCACACATATCCAAGCAATTCCTCAAACACCTAATTAGTTTCACAAAGCGAGCGATGCAATCTCAACATGTGAAGACGATCGCGCGGCTGCCATGGACGATTCTCTCCACGATCCCTCCATTCGCAGTGTTAAAACAGATTCTATTCTTCAGAGAGCAACAAGAAGCCAAGGAAGAAACAGAATCTCAAAACCCTAATTCAAATCCAAACGCAAATAGACCGCCATTAACAGAGGAAATAACAATACCTTCCGTTTCGGAGCTCTTCAAAGCCGGAATCCGCTTCTCACCCTCCCATGGCGGAATCTCTACAATCACCTTCAACGCCAAAACCGGAATGCTGTACCTTCCAACGGCGAGACTCGACGTGAACACACAAGCAACGCTGAGAAACCTCGTAGCATACGAAGCGTCAAGCGGATTAGGGGCGCTGGTTTTCACAAGATACACGGAATTGATGAACGGAATCGTGGATTCCAACGAGGATTTGAGATTGCTAAGAGAAGGAGGGGTGATTGTGAACCATCTGAAGAGCGACGAAGAGGGTGCTGCGATGTGGAATGGGATGAGCAAATCGATCAGATTGAGCAGAGTATTGGAGTTGGACAAGGTGATTGAAGAGGTGAATGAGTATTACAATGGATTATGGAGAGTGAAAATGGGAAGATTTGTGAAGGAATATGTTTACAGTTCTTGGCCTTTGCTTGCTTTAATGGCGGTCGCTTGGTTTCTGTTCTTGATCGCTCTCCAAGCTTTTTGCTCTCTGTTTGGTTGCTTTCGCGCCCTTCCTCGCAAGGGTTCTTGA

Coding sequence (CDS)

ATGGCATCCAATTCTTCGTCTTCGACCTTCGACGAGCTCAAATGGGTCATTCAAATTCGTCGGACCCTCGAAGAAGAACTCGACGACGACGACGGGGGGATTCCAGTAACAATCTTCAAGGTCCCTAAATTGTTAATGGCGTCCGATCCACATTCCTACACTCCACAATTGGTTGCGTTGGGTCCTTACCATTGTTGGCGTCCGGAGCTCCGTGAGATGGACAGATTCAAGCTCTCTTCTGCTAAAAGGGCTAAGAAATTCAGCCCTATAAACCTCCAATTTCACGATCTCGTTGAACAATTGTCGAAGCTCCAGCAGAAGATTCGAGCGTGTTACCACGGTTACTTGGATCTTAATGCCGAAACTTTAGCCTGGATGATGGCCGTTGATGCCTCTTTCTTGCTTGAGTTTCTCCATGTCTACGCGGGAGAATTGAAGAGTCTCCGGCGAGTTTCTTCTAGAATGTCGCATTTGATGGATTACGCCGGGACGAAGCCGGCACATAACGCTGTTCTCAGAGATATAATCATGCTCGAAAATCAAATTCCATTGTTCGTATTGAGGAAGATGATGGAATTCCAATTCGAATCCTCTGAACAATCTGATGAGATTTTGCTCTCCATGATGAACGGTTTCTTCACAGAGCTTTCTCCATTCAACACCACGGACGGGATTCCAGATATTCAAATTTCAGATTGCGCTCATTTACTGGACTTTTTATACCAAATGATCACTCCTAAATCGAAAGAACCATCAGAAATTATCGAAATCCCTAATGAAATCGAACAATCTCCCCAAATTTCGAGTTACAGAAAACGATTCGCACACATATCCAAGCAATTCCTCAAACACCTAATTAGTTTCACAAAGCGAGCGATGCAATCTCAACATGTGAAGACGATCGCGCGGCTGCCATGGACGATTCTCTCCACGATCCCTCCATTCGCAGTGTTAAAACAGATTCTATTCTTCAGAGAGCAACAAGAAGCCAAGGAAGAAACAGAATCTCAAAACCCTAATTCAAATCCAAACGCAAATAGACCGCCATTAACAGAGGAAATAACAATACCTTCCGTTTCGGAGCTCTTCAAAGCCGGAATCCGCTTCTCACCCTCCCATGGCGGAATCTCTACAATCACCTTCAACGCCAAAACCGGAATGCTGTACCTTCCAACGGCGAGACTCGACGTGAACACACAAGCAACGCTGAGAAACCTCGTAGCATACGAAGCGTCAAGCGGATTAGGGGCGCTGGTTTTCACAAGATACACGGAATTGATGAACGGAATCGTGGATTCCAACGAGGATTTGAGATTGCTAAGAGAAGGAGGGGTGATTGTGAACCATCTGAAGAGCGACGAAGAGGGTGCTGCGATGTGGAATGGGATGAGCAAATCGATCAGATTGAGCAGAGTATTGGAGTTGGACAAGGTGATTGAAGAGGTGAATGAGTATTACAATGGATTATGGAGAGTGAAAATGGGAAGATTTGTGAAGGAATATGTTTACAGTTCTTGGCCTTTGCTTGCTTTAATGGCGGTCGCTTGGTTTCTGTTCTTGATCGCTCTCCAAGCTTTTTGCTCTCTGTTTGGTTGCTTTCGCGCCCTTCCTCGCAAGGGTTCTTGA

Protein sequence

MASNSSSSTFDELKWVIQIRRTLEEELDDDDGGIPVTIFKVPKLLMASDPHSYTPQLVALGPYHCWRPELREMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLNAETLAWMMAVDASFLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLENQIPLFVLRKMMEFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDFLYQMITPKSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFLKHLISFTKRAMQSQHVKTIARLPWTILSTIPPFAVLKQILFFREQQEAKEETESQNPNSNPNANRPPLTEEITIPSVSELFKAGIRFSPSHGGISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASSGLGALVFTRYTELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVLELDKVIEEVNEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCFRALPRKGS
Homology
BLAST of Lsi07G004320 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 2.8e-134
Identity = 260/539 (48.24%), Postives = 357/539 (66.23%), Query Frame = 0

Query: 3   SNSSSSTFDELKWVIQIRRTLEEELDDDD-GGIPVTIFKVPKLLMASDPHSYTPQLVALG 62
           S++    FDE +WVI ++++L+ EL++ D   + V+IF VPK LM S P SYTP  V++G
Sbjct: 9   SSTEQHRFDETRWVINVQKSLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIG 68

Query: 63  PYHCWRPELREMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLNAE 122
           PYHC +PEL EM+R+KL  A++ +     + +FHDLVE+L  ++ KIRACYH Y+  N E
Sbjct: 69  PYHCLKPELHEMERYKLMIARKIRN-QYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGE 128

Query: 123 TLAWMMAVDASFLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLENQ 182
           TL W+MAVD+SFL+EFL +Y     S R+V + ++ +         HN +LRDI+M+ENQ
Sbjct: 129 TLLWIMAVDSSFLIEFLKIY-----SFRKVETLINRV--------GHNEILRDIMMIENQ 188

Query: 183 IPLFVLRKMMEFQFESSEQSDEILLSMMNGFFTELSPF---NTTDGIPDIQISDCAHLLD 242
           IPLFVLRK +EFQ ES+E +D++LLS++ G   +LSP       D I   Q  +C H+LD
Sbjct: 189 IPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNHILD 248

Query: 243 FLYQMITPKSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFLKHLISFTKRAMQSQHV 302
           FLYQMI P+ +E         E+E+  + +   +   + + +F+  +    KR   S+  
Sbjct: 249 FLYQMIVPRIEE--------EELEEDDEENRADENGGNRAIRFMDEIKHQFKRVFASRPA 308

Query: 303 KTIARLPWTILSTIPPFAVLK---QILFFREQQEAKEETESQNPNSNPNANRPPLTEEIT 362
             I R PW I+S +P F  LK     LF R++ EA   T  Q   S  +  +PPL EE+T
Sbjct: 309 DLILRFPWRIISNLPGFMALKLSADYLFTRQENEA--TTTRQESVSILDIEKPPLVEELT 368

Query: 363 IPSVSELFKAGIRFSP-SHGGISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASSG 422
           IPSVS+L KAG+RF P +HG IST+TF++ +G  YLP   LD+NT+  LRNLVAYEA++ 
Sbjct: 369 IPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNT 428

Query: 423 LGALVFTRYTELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVLE 482
            G LVFTRYTEL+NGI+DS ED+RLLRE GV+V+ LKSD+E A MWNGMSKS+RL++V  
Sbjct: 429 SGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSKSVRLTKVGF 488

Query: 483 LDKVIEEVNEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCF 534
           LDK IE+VN YY G W+VK+GR V+ YVY SW +LA +A    L L++LQ F  +F  F
Sbjct: 489 LDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFLAAVLLLMLVSLQLFSLVFSSF 523

BLAST of Lsi07G004320 vs. ExPASy TrEMBL
Match: A0A0A0KYJ3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G006280 PE=4 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 2.2e-259
Identity = 467/545 (85.69%), Postives = 493/545 (90.46%), Query Frame = 0

Query: 1   MASNSSSSTFDELKWVIQIRRTLEEEL-DDDDGGIPVTIFKVPKLLMASDPHSYTPQLVA 60
           M SN SSSTFDELKWVIQIRRTLEEEL DD+D GIPV IFKVPKLLM SDPHSYTPQLVA
Sbjct: 1   MVSNYSSSTFDELKWVIQIRRTLEEELEDDEDYGIPVAIFKVPKLLMESDPHSYTPQLVA 60

Query: 61  LGPYHCWRPELREMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLN 120
           LGPYHCWRPEL +M+R KLS+AKRAKKFS  NLQFH+LV+Q+SK++QKIRACYHGYLD+N
Sbjct: 61  LGPYHCWRPELHDMERLKLSAAKRAKKFSSTNLQFHELVQQMSKIEQKIRACYHGYLDIN 120

Query: 121 AETLAWMMAVDASFLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLE 180
            ETLAWMMA+DASFLLEFL VYAGE K LRRVSSRMSHLMD+AGTKPAHNAVLRDIIMLE
Sbjct: 121 GETLAWMMAIDASFLLEFLLVYAGEFKILRRVSSRMSHLMDHAGTKPAHNAVLRDIIMLE 180

Query: 181 NQIPLFVLRKMMEFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDF 240
           NQIPLFVLRKMMEFQ ESS+QSDEILLSMM G FTELSPFNTTDGIP+IQISDCAHLLDF
Sbjct: 181 NQIPLFVLRKMMEFQSESSQQSDEILLSMMIGLFTELSPFNTTDGIPEIQISDCAHLLDF 240

Query: 241 LYQMITPKSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFLKHLISFTKRAMQSQHVK 300
           LYQMI PKSKE SEI E PNEI+QSPQIS       H  KQFLK LISFTKRA+QSQHVK
Sbjct: 241 LYQMIAPKSKESSEINETPNEIDQSPQIS-------HRFKQFLKRLISFTKRAIQSQHVK 300

Query: 301 TIARLPWTILSTIPPFAVLKQ---ILFFREQQEAKEETESQNPNSNPNANRPPLTEEITI 360
           TIARLPWTILSTIPPF VLKQ   ILFFREQQEAKEE E QNPNS  NANRPPLTEEI I
Sbjct: 301 TIARLPWTILSTIPPFTVLKQPAEILFFREQQEAKEEPEPQNPNS--NANRPPLTEEIAI 360

Query: 361 PSVSELFKAGIRFSPSHGGISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASSGLG 420
           PSVSEL KAGI FSPSHGGISTI F+++TG LYLPTARLDVNT+ATLRNLVAYEASSG+G
Sbjct: 361 PSVSELSKAGIHFSPSHGGISTINFDSQTGTLYLPTARLDVNTEATLRNLVAYEASSGIG 420

Query: 421 ALVFTRYTELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVLELD 480
           ALV TRYTELMNGIVD+ ED+RLLREGGVIVNHLKSDEEGA MWNGMSKSIRLSRV+ELD
Sbjct: 421 ALVLTRYTELMNGIVDTEEDVRLLREGGVIVNHLKSDEEGAWMWNGMSKSIRLSRVMELD 480

Query: 481 KVIEEVNEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCFRAL 540
           KVIEEVNEYYNGLWRVKMGRFVKEY+YSSWPL+AL  VAWFLFLI LQAFCSLFGCFRAL
Sbjct: 481 KVIEEVNEYYNGLWRVKMGRFVKEYIYSSWPLIALTTVAWFLFLIGLQAFCSLFGCFRAL 536

Query: 541 PRKGS 542
           P K S
Sbjct: 541 PSKRS 536

BLAST of Lsi07G004320 vs. ExPASy TrEMBL
Match: A0A5A7TLR9 (Putative UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001990 PE=4 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 4.2e-258
Identity = 470/545 (86.24%), Postives = 491/545 (90.09%), Query Frame = 0

Query: 1   MASNSSSSTFDELKWVIQIRRTLEEEL-DDDDGGIPVTIFKVPKLLMASDPHSYTPQLVA 60
           M SN SSSTFDELKWVIQIRRTLEEE  DD+D GIPV IFKVPKLLM SDPHSY PQLVA
Sbjct: 1   MPSNYSSSTFDELKWVIQIRRTLEEEQEDDEDYGIPVAIFKVPKLLMESDPHSYIPQLVA 60

Query: 61  LGPYHCWRPELREMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLN 120
           LGPYHCWRPEL +M R KLS+AKRAKKFS  NLQFHDLVEQL KL+Q IRACYHGYLDLN
Sbjct: 61  LGPYHCWRPELHDMVRLKLSAAKRAKKFSSTNLQFHDLVEQLLKLEQNIRACYHGYLDLN 120

Query: 121 AETLAWMMAVDASFLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLE 180
            ETLAWMMA+DASFLLEFLHVYAG+ K L+RV SRMSHLMD+AGTKPAHNAVLRDIIMLE
Sbjct: 121 GETLAWMMAIDASFLLEFLHVYAGKFKILQRVPSRMSHLMDHAGTKPAHNAVLRDIIMLE 180

Query: 181 NQIPLFVLRKMMEFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDF 240
           NQIPLFVLRKMMEFQFESS+QSDEILLSMM G FTELSPFNTTDGIP+IQISDCAHLLDF
Sbjct: 181 NQIPLFVLRKMMEFQFESSQQSDEILLSMMIGLFTELSPFNTTDGIPEIQISDCAHLLDF 240

Query: 241 LYQMITPKSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFLKHLISFTKRAMQSQHVK 300
           LYQMI PKSKE SEI EIPNEIEQSPQI SYR       KQFLK LIS+TKRAMQSQH+K
Sbjct: 241 LYQMIAPKSKEASEINEIPNEIEQSPQI-SYR------FKQFLKCLISYTKRAMQSQHIK 300

Query: 301 TIARLPWTILSTIPPFAVLKQ---ILFFREQQEAKEETESQNPNSNPNANRPPLTEEITI 360
           TIARLPWTILSTIPPF VLKQ   ILFFREQQE +EE E QN  S  NANRPPLTEEI I
Sbjct: 301 TIARLPWTILSTIPPFTVLKQPAEILFFREQQEPREEPEPQNLKS--NANRPPLTEEIAI 360

Query: 361 PSVSELFKAGIRFSPSHGGISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASSGLG 420
           PSVSEL KAGIRFSPSHGGISTITFN++TG LYLPTARLDVNT+ATLRNLVAYEASSG+G
Sbjct: 361 PSVSELSKAGIRFSPSHGGISTITFNSQTGTLYLPTARLDVNTEATLRNLVAYEASSGIG 420

Query: 421 ALVFTRYTELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVLELD 480
           ALV TRYTELMNGIVDS ED+RLLREGGVIVNHLKSDEEGA MWNGMSKSIRLSRV+ELD
Sbjct: 421 ALVLTRYTELMNGIVDSEEDVRLLREGGVIVNHLKSDEEGAWMWNGMSKSIRLSRVMELD 480

Query: 481 KVIEEVNEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCFRAL 540
           KVIEEVNEYYNGLWRVKMGRFVKEY+YSSWPL+AL  VAWFLFLIALQAFCSLFGCFRAL
Sbjct: 481 KVIEEVNEYYNGLWRVKMGRFVKEYIYSSWPLIALTTVAWFLFLIALQAFCSLFGCFRAL 536

Query: 541 PRKGS 542
           P K S
Sbjct: 541 PSKRS 536

BLAST of Lsi07G004320 vs. ExPASy TrEMBL
Match: A0A1S3BYU2 (putative UPF0481 protein At3g02645 OS=Cucumis melo OX=3656 GN=LOC103494677 PE=4 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 4.2e-258
Identity = 470/545 (86.24%), Postives = 491/545 (90.09%), Query Frame = 0

Query: 1   MASNSSSSTFDELKWVIQIRRTLEEEL-DDDDGGIPVTIFKVPKLLMASDPHSYTPQLVA 60
           M SN SSSTFDELKWVIQIRRTLEEE  DD+D GIPV IFKVPKLLM SDPHSY PQLVA
Sbjct: 1   MPSNYSSSTFDELKWVIQIRRTLEEEQEDDEDYGIPVAIFKVPKLLMESDPHSYIPQLVA 60

Query: 61  LGPYHCWRPELREMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLN 120
           LGPYHCWRPEL +M R KLS+AKRAKKFS  NLQFHDLVEQL KL+Q IRACYHGYLDLN
Sbjct: 61  LGPYHCWRPELHDMVRLKLSAAKRAKKFSSTNLQFHDLVEQLLKLEQNIRACYHGYLDLN 120

Query: 121 AETLAWMMAVDASFLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLE 180
            ETLAWMMA+DASFLLEFLHVYAG+ K L+RV SRMSHLMD+AGTKPAHNAVLRDIIMLE
Sbjct: 121 GETLAWMMAIDASFLLEFLHVYAGKFKILQRVPSRMSHLMDHAGTKPAHNAVLRDIIMLE 180

Query: 181 NQIPLFVLRKMMEFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDF 240
           NQIPLFVLRKMMEFQFESS+QSDEILLSMM G FTELSPFNTTDGIP+IQISDCAHLLDF
Sbjct: 181 NQIPLFVLRKMMEFQFESSQQSDEILLSMMIGLFTELSPFNTTDGIPEIQISDCAHLLDF 240

Query: 241 LYQMITPKSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFLKHLISFTKRAMQSQHVK 300
           LYQMI PKSKE SEI EIPNEIEQSPQI SYR       KQFLK LIS+TKRAMQSQH+K
Sbjct: 241 LYQMIAPKSKEASEINEIPNEIEQSPQI-SYR------FKQFLKCLISYTKRAMQSQHIK 300

Query: 301 TIARLPWTILSTIPPFAVLKQ---ILFFREQQEAKEETESQNPNSNPNANRPPLTEEITI 360
           TIARLPWTILSTIPPF VLKQ   ILFFREQQE +EE E QN  S  NANRPPLTEEI I
Sbjct: 301 TIARLPWTILSTIPPFTVLKQPAEILFFREQQEPREEPEPQNLKS--NANRPPLTEEIAI 360

Query: 361 PSVSELFKAGIRFSPSHGGISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASSGLG 420
           PSVSEL KAGIRFSPSHGGISTITFN++TG LYLPTARLDVNT+ATLRNLVAYEASSG+G
Sbjct: 361 PSVSELSKAGIRFSPSHGGISTITFNSQTGTLYLPTARLDVNTEATLRNLVAYEASSGIG 420

Query: 421 ALVFTRYTELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVLELD 480
           ALV TRYTELMNGIVDS ED+RLLREGGVIVNHLKSDEEGA MWNGMSKSIRLSRV+ELD
Sbjct: 421 ALVLTRYTELMNGIVDSEEDVRLLREGGVIVNHLKSDEEGAWMWNGMSKSIRLSRVMELD 480

Query: 481 KVIEEVNEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCFRAL 540
           KVIEEVNEYYNGLWRVKMGRFVKEY+YSSWPL+AL  VAWFLFLIALQAFCSLFGCFRAL
Sbjct: 481 KVIEEVNEYYNGLWRVKMGRFVKEYIYSSWPLIALTTVAWFLFLIALQAFCSLFGCFRAL 536

Query: 541 PRKGS 542
           P K S
Sbjct: 541 PSKRS 536

BLAST of Lsi07G004320 vs. ExPASy TrEMBL
Match: A0A6J1GW24 (putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111458007 PE=4 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 7.9e-257
Identity = 466/546 (85.35%), Postives = 494/546 (90.48%), Query Frame = 0

Query: 1   MASNSSSSTFDELKWVIQIRRTLEEELDDDDGGIPVTIFKVPKLLMASDPHSYTPQLVAL 60
           MASNS SSTFDE  WVIQIRRTLEEELDDDD GIPV+IFKVPK LM SDP SYTPQLVAL
Sbjct: 1   MASNSFSSTFDECNWVIQIRRTLEEELDDDD-GIPVSIFKVPKSLMVSDPDSYTPQLVAL 60

Query: 61  GPYHCWRPELREMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLNA 120
           GPYHCWRPEL EM+R KLS+AKRAKKFSP +LQFHDLVEQL+KL+ KIRA YHGYLDLN+
Sbjct: 61  GPYHCWRPELHEMERIKLSAAKRAKKFSPRDLQFHDLVEQLAKLEHKIRAYYHGYLDLNS 120

Query: 121 ETLAWMMAVDASFLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLEN 180
           ETLAWMMA+DA FLLEFL+VYA +LK LRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLEN
Sbjct: 121 ETLAWMMAIDACFLLEFLNVYAQQLKILRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLEN 180

Query: 181 QIPLFVLRKMMEFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDFL 240
           QIPLFVLRKMMEFQFES+EQSDE LLSM+ G F ELSPFNTTD IP+IQISDCAHLLDFL
Sbjct: 181 QIPLFVLRKMMEFQFESTEQSDETLLSMLIGLFAELSPFNTTDEIPEIQISDCAHLLDFL 240

Query: 241 YQMITPKSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFL----KHLISFTKRAMQSQ 300
           YQMITPK KEPSEI E PNEIEQSPQI SYR++ AH SKQ L    ++LI FTKRAMQSQ
Sbjct: 241 YQMITPKLKEPSEITENPNEIEQSPQILSYRQQIAHRSKQCLLRSIENLIRFTKRAMQSQ 300

Query: 301 HVKTIARLPWTILSTIPPFAVLKQ---ILFFREQQEAKEETESQNPNSNPNANRPPLTEE 360
           HVKTIARLPW ILS+IPPFA+LKQ   ILFFREQ     ETE QNP+SNPNANRPPLTEE
Sbjct: 301 HVKTIARLPWAILSSIPPFAILKQPAEILFFREQ-----ETEPQNPDSNPNANRPPLTEE 360

Query: 361 ITIPSVSELFKAGIRFSPSHGGISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASS 420
           ITIPSVSELFKAGIRFSPS GGISTITF+  TG LYLPT RLDVNTQATLRNLVAYEASS
Sbjct: 361 ITIPSVSELFKAGIRFSPSDGGISTITFDRNTGTLYLPTVRLDVNTQATLRNLVAYEASS 420

Query: 421 GLGALVFTRYTELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVL 480
           GLG LV TRYTELMNGIVDS+ED+RLLREGGVI+NHLKSDEEGAAMWNGMSKS+RLSRVL
Sbjct: 421 GLGPLVLTRYTELMNGIVDSDEDVRLLREGGVILNHLKSDEEGAAMWNGMSKSMRLSRVL 480

Query: 481 ELDKVIEEVNEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCF 540
           ELDKVIEEVNEYY+GLWRVKMGRFV+ Y+YSSWPLLALMAVAW LFLIALQAFCSLFGCF
Sbjct: 481 ELDKVIEEVNEYYDGLWRVKMGRFVEAYIYSSWPLLALMAVAWVLFLIALQAFCSLFGCF 540

BLAST of Lsi07G004320 vs. ExPASy TrEMBL
Match: A0A6J1IWY7 (putative UPF0481 protein At3g02645 OS=Cucurbita maxima OX=3661 GN=LOC111479236 PE=4 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 1.1e-253
Identity = 461/543 (84.90%), Postives = 491/543 (90.42%), Query Frame = 0

Query: 1   MASNSSSSTFDELKWVIQIRRTLEEELDDDDGGIPVTIFKVPKLLMASDPHSYTPQLVAL 60
           MASNS SSTFDE  WVIQIRRTLEEELDDDD GIPV+IFKVPK LMASDP SYTPQLVAL
Sbjct: 1   MASNSYSSTFDECNWVIQIRRTLEEELDDDD-GIPVSIFKVPKSLMASDPDSYTPQLVAL 60

Query: 61  GPYHCWRPELREMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLNA 120
           GPYHCW PEL EM+R KLS+AKRAKKFSP +L+F D+VEQL+KL+ KIRA YHGYLDLN+
Sbjct: 61  GPYHCWCPELHEMERIKLSAAKRAKKFSPRDLRFDDIVEQLAKLEHKIRAYYHGYLDLNS 120

Query: 121 ETLAWMMAVDASFLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLEN 180
           ETLAWMMA+DA FLLEFL+VYA +LK LRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLEN
Sbjct: 121 ETLAWMMAIDACFLLEFLNVYAQQLKILRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLEN 180

Query: 181 QIPLFVLRKMMEFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDFL 240
           QIPLFVLRKMMEFQFES+EQSDE LLSM+ G F ELSPFNTTD IP+IQISDCAHLLDFL
Sbjct: 181 QIPLFVLRKMMEFQFESTEQSDETLLSMLIGLFAELSPFNTTDEIPEIQISDCAHLLDFL 240

Query: 241 YQMITPKSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFL----KHLISFTKRAMQSQ 300
           YQMITPK KEPSEI E PNEIEQSPQI SYR++ AH SKQ L    ++LI FTKRAMQSQ
Sbjct: 241 YQMITPKLKEPSEITENPNEIEQSPQILSYRQQVAHRSKQCLLRSIENLIRFTKRAMQSQ 300

Query: 301 HVKTIARLPWTILSTIPPFAVLKQ---ILFFREQQEAKEETESQNPNSNPNANRPPLTEE 360
           HVKTIARLPW ILS+IPPFA+LKQ   ILFFREQ     ETE QNP+SNPNANRPPLTEE
Sbjct: 301 HVKTIARLPWAILSSIPPFAILKQPAEILFFREQ-----ETEPQNPDSNPNANRPPLTEE 360

Query: 361 ITIPSVSELFKAGIRFSPSHGGISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASS 420
           ITIPSVSELFKAGIRFSPSHGGISTITF+  TG LYLPT RLDVNTQATLRNLVAYEASS
Sbjct: 361 ITIPSVSELFKAGIRFSPSHGGISTITFDRNTGTLYLPTVRLDVNTQATLRNLVAYEASS 420

Query: 421 GLGALVFTRYTELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVL 480
           GLG LV TRYTELMNGIVDS+ED+RLLREGGVI+NHLKSDEEGAAMWNGMSKS+RLSRVL
Sbjct: 421 GLGPLVLTRYTELMNGIVDSDEDVRLLREGGVILNHLKSDEEGAAMWNGMSKSMRLSRVL 480

Query: 481 ELDKVIEEVNEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCF 537
           ELDKVIEEVNEYY+GLWRVKMGRFV+ Y+YSSWPLL LMAVAW LFLIALQAFCSLFGCF
Sbjct: 481 ELDKVIEEVNEYYDGLWRVKMGRFVEAYIYSSWPLLGLMAVAWVLFLIALQAFCSLFGCF 537

BLAST of Lsi07G004320 vs. NCBI nr
Match: XP_038890629.1 (putative UPF0481 protein At3g02645 [Benincasa hispida])

HSP 1 Score: 956.1 bits (2470), Expect = 1.3e-274
Identity = 492/544 (90.44%), Postives = 505/544 (92.83%), Query Frame = 0

Query: 1   MASNSSSSTFDELKWVIQIRRTLEEELDDDDGGIPVTIFKVPKLLMASDPHSYTPQLVAL 60
           MASNSSSSTFDELKWVIQIRRTLEEELDDDD GIP+ IFKVPKLLMASDPHSYTPQLVAL
Sbjct: 1   MASNSSSSTFDELKWVIQIRRTLEEELDDDDDGIPIAIFKVPKLLMASDPHSYTPQLVAL 60

Query: 61  GPYHCWRPELREMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLNA 120
           GPYHCWRPEL EMDR KLS+AKRAKKFSP +LQFHDLVEQLSKL+QKIRACYHGYLDLN 
Sbjct: 61  GPYHCWRPELHEMDRCKLSAAKRAKKFSPTSLQFHDLVEQLSKLEQKIRACYHGYLDLNG 120

Query: 121 ETLAWMMAVDASFLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLEN 180
           ETLAWMMA+DASFLLEFLHVYAGELK LRRVSS MSHLMDYAGTKP HNAVLRD IMLEN
Sbjct: 121 ETLAWMMAIDASFLLEFLHVYAGELKILRRVSSGMSHLMDYAGTKPTHNAVLRDTIMLEN 180

Query: 181 QIPLFVLRKMMEFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDFL 240
           QIPLFVLRKMMEFQFESSEQSDEILLSMM G FTELSPFNTTDGIP+IQISDCAHLLDFL
Sbjct: 181 QIPLFVLRKMMEFQFESSEQSDEILLSMMIGLFTELSPFNTTDGIPEIQISDCAHLLDFL 240

Query: 241 YQMITPKSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFLKHLISFTKRAMQSQHVKT 300
           YQMITPKSK PSEIIEIPNEIEQSPQI        H SKQFLK LI FTKRAMQSQH+KT
Sbjct: 241 YQMITPKSKGPSEIIEIPNEIEQSPQI-------LHRSKQFLKRLIRFTKRAMQSQHMKT 300

Query: 301 IARLPWTILSTIPPFAVLKQ---ILFFREQQEAKEETESQNPNSNPNANRPPLTEEITIP 360
           I RLPWTIL TIPPF +LKQ   ILFF+EQQEAKEETESQNPNSNPNANRPPLTEEITIP
Sbjct: 301 ITRLPWTILYTIPPFTILKQPAEILFFKEQQEAKEETESQNPNSNPNANRPPLTEEITIP 360

Query: 361 SVSELFKAGIRFSPSHGGISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASSGLGA 420
           SVSELF+AGIRFSPSHGGISTITFNAKTG LYLPTARLDVNTQATLRNLVAYEASSG+GA
Sbjct: 361 SVSELFRAGIRFSPSHGGISTITFNAKTGTLYLPTARLDVNTQATLRNLVAYEASSGIGA 420

Query: 421 LVFTRYTELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVLELDK 480
           LV TRYTELMNGIVDS+EDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVLELDK
Sbjct: 421 LVLTRYTELMNGIVDSDEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVLELDK 480

Query: 481 VIEEVNEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCFRALP 540
           VIEEVNEYYNGLWRVKMGRFVKEY+YSSWPLLALMAVAWFLF IALQAFCSLFG  R L 
Sbjct: 481 VIEEVNEYYNGLWRVKMGRFVKEYIYSSWPLLALMAVAWFLFFIALQAFCSLFG--RLLS 535

Query: 541 RKGS 542
           R  S
Sbjct: 541 RPSS 535

BLAST of Lsi07G004320 vs. NCBI nr
Match: KAE8649063.1 (hypothetical protein Csa_014591 [Cucumis sativus])

HSP 1 Score: 904.4 bits (2336), Expect = 4.6e-259
Identity = 467/545 (85.69%), Postives = 493/545 (90.46%), Query Frame = 0

Query: 1   MASNSSSSTFDELKWVIQIRRTLEEEL-DDDDGGIPVTIFKVPKLLMASDPHSYTPQLVA 60
           M SN SSSTFDELKWVIQIRRTLEEEL DD+D GIPV IFKVPKLLM SDPHSYTPQLVA
Sbjct: 8   MVSNYSSSTFDELKWVIQIRRTLEEELEDDEDYGIPVAIFKVPKLLMESDPHSYTPQLVA 67

Query: 61  LGPYHCWRPELREMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLN 120
           LGPYHCWRPEL +M+R KLS+AKRAKKFS  NLQFH+LV+Q+SK++QKIRACYHGYLD+N
Sbjct: 68  LGPYHCWRPELHDMERLKLSAAKRAKKFSSTNLQFHELVQQMSKIEQKIRACYHGYLDIN 127

Query: 121 AETLAWMMAVDASFLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLE 180
            ETLAWMMA+DASFLLEFL VYAGE K LRRVSSRMSHLMD+AGTKPAHNAVLRDIIMLE
Sbjct: 128 GETLAWMMAIDASFLLEFLLVYAGEFKILRRVSSRMSHLMDHAGTKPAHNAVLRDIIMLE 187

Query: 181 NQIPLFVLRKMMEFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDF 240
           NQIPLFVLRKMMEFQ ESS+QSDEILLSMM G FTELSPFNTTDGIP+IQISDCAHLLDF
Sbjct: 188 NQIPLFVLRKMMEFQSESSQQSDEILLSMMIGLFTELSPFNTTDGIPEIQISDCAHLLDF 247

Query: 241 LYQMITPKSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFLKHLISFTKRAMQSQHVK 300
           LYQMI PKSKE SEI E PNEI+QSPQIS       H  KQFLK LISFTKRA+QSQHVK
Sbjct: 248 LYQMIAPKSKESSEINETPNEIDQSPQIS-------HRFKQFLKRLISFTKRAIQSQHVK 307

Query: 301 TIARLPWTILSTIPPFAVLKQ---ILFFREQQEAKEETESQNPNSNPNANRPPLTEEITI 360
           TIARLPWTILSTIPPF VLKQ   ILFFREQQEAKEE E QNPNS  NANRPPLTEEI I
Sbjct: 308 TIARLPWTILSTIPPFTVLKQPAEILFFREQQEAKEEPEPQNPNS--NANRPPLTEEIAI 367

Query: 361 PSVSELFKAGIRFSPSHGGISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASSGLG 420
           PSVSEL KAGI FSPSHGGISTI F+++TG LYLPTARLDVNT+ATLRNLVAYEASSG+G
Sbjct: 368 PSVSELSKAGIHFSPSHGGISTINFDSQTGTLYLPTARLDVNTEATLRNLVAYEASSGIG 427

Query: 421 ALVFTRYTELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVLELD 480
           ALV TRYTELMNGIVD+ ED+RLLREGGVIVNHLKSDEEGA MWNGMSKSIRLSRV+ELD
Sbjct: 428 ALVLTRYTELMNGIVDTEEDVRLLREGGVIVNHLKSDEEGAWMWNGMSKSIRLSRVMELD 487

Query: 481 KVIEEVNEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCFRAL 540
           KVIEEVNEYYNGLWRVKMGRFVKEY+YSSWPL+AL  VAWFLFLI LQAFCSLFGCFRAL
Sbjct: 488 KVIEEVNEYYNGLWRVKMGRFVKEYIYSSWPLIALTTVAWFLFLIGLQAFCSLFGCFRAL 543

Query: 541 PRKGS 542
           P K S
Sbjct: 548 PSKRS 543

BLAST of Lsi07G004320 vs. NCBI nr
Match: XP_004152335.1 (putative UPF0481 protein At3g02645 [Cucumis sativus])

HSP 1 Score: 904.4 bits (2336), Expect = 4.6e-259
Identity = 467/545 (85.69%), Postives = 493/545 (90.46%), Query Frame = 0

Query: 1   MASNSSSSTFDELKWVIQIRRTLEEEL-DDDDGGIPVTIFKVPKLLMASDPHSYTPQLVA 60
           M SN SSSTFDELKWVIQIRRTLEEEL DD+D GIPV IFKVPKLLM SDPHSYTPQLVA
Sbjct: 1   MVSNYSSSTFDELKWVIQIRRTLEEELEDDEDYGIPVAIFKVPKLLMESDPHSYTPQLVA 60

Query: 61  LGPYHCWRPELREMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLN 120
           LGPYHCWRPEL +M+R KLS+AKRAKKFS  NLQFH+LV+Q+SK++QKIRACYHGYLD+N
Sbjct: 61  LGPYHCWRPELHDMERLKLSAAKRAKKFSSTNLQFHELVQQMSKIEQKIRACYHGYLDIN 120

Query: 121 AETLAWMMAVDASFLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLE 180
            ETLAWMMA+DASFLLEFL VYAGE K LRRVSSRMSHLMD+AGTKPAHNAVLRDIIMLE
Sbjct: 121 GETLAWMMAIDASFLLEFLLVYAGEFKILRRVSSRMSHLMDHAGTKPAHNAVLRDIIMLE 180

Query: 181 NQIPLFVLRKMMEFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDF 240
           NQIPLFVLRKMMEFQ ESS+QSDEILLSMM G FTELSPFNTTDGIP+IQISDCAHLLDF
Sbjct: 181 NQIPLFVLRKMMEFQSESSQQSDEILLSMMIGLFTELSPFNTTDGIPEIQISDCAHLLDF 240

Query: 241 LYQMITPKSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFLKHLISFTKRAMQSQHVK 300
           LYQMI PKSKE SEI E PNEI+QSPQIS       H  KQFLK LISFTKRA+QSQHVK
Sbjct: 241 LYQMIAPKSKESSEINETPNEIDQSPQIS-------HRFKQFLKRLISFTKRAIQSQHVK 300

Query: 301 TIARLPWTILSTIPPFAVLKQ---ILFFREQQEAKEETESQNPNSNPNANRPPLTEEITI 360
           TIARLPWTILSTIPPF VLKQ   ILFFREQQEAKEE E QNPNS  NANRPPLTEEI I
Sbjct: 301 TIARLPWTILSTIPPFTVLKQPAEILFFREQQEAKEEPEPQNPNS--NANRPPLTEEIAI 360

Query: 361 PSVSELFKAGIRFSPSHGGISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASSGLG 420
           PSVSEL KAGI FSPSHGGISTI F+++TG LYLPTARLDVNT+ATLRNLVAYEASSG+G
Sbjct: 361 PSVSELSKAGIHFSPSHGGISTINFDSQTGTLYLPTARLDVNTEATLRNLVAYEASSGIG 420

Query: 421 ALVFTRYTELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVLELD 480
           ALV TRYTELMNGIVD+ ED+RLLREGGVIVNHLKSDEEGA MWNGMSKSIRLSRV+ELD
Sbjct: 421 ALVLTRYTELMNGIVDTEEDVRLLREGGVIVNHLKSDEEGAWMWNGMSKSIRLSRVMELD 480

Query: 481 KVIEEVNEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCFRAL 540
           KVIEEVNEYYNGLWRVKMGRFVKEY+YSSWPL+AL  VAWFLFLI LQAFCSLFGCFRAL
Sbjct: 481 KVIEEVNEYYNGLWRVKMGRFVKEYIYSSWPLIALTTVAWFLFLIGLQAFCSLFGCFRAL 536

Query: 541 PRKGS 542
           P K S
Sbjct: 541 PSKRS 536

BLAST of Lsi07G004320 vs. NCBI nr
Match: KAG6581946.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 901.0 bits (2327), Expect = 5.1e-258
Identity = 468/546 (85.71%), Postives = 495/546 (90.66%), Query Frame = 0

Query: 1   MASNSSSSTFDELKWVIQIRRTLEEELDDDDGGIPVTIFKVPKLLMASDPHSYTPQLVAL 60
           MASNS SSTFDE  WVIQIRRTLEEELDDDD GIPV+IFKVPK LMASDP SYTPQLVAL
Sbjct: 1   MASNSFSSTFDECNWVIQIRRTLEEELDDDD-GIPVSIFKVPKSLMASDPDSYTPQLVAL 60

Query: 61  GPYHCWRPELREMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLNA 120
           GPYHCWRPEL EM+R KLS+AKRAKKFSP +LQFHDLVEQL+KL+ KIRA YHGYLDLN+
Sbjct: 61  GPYHCWRPELHEMERIKLSAAKRAKKFSPRDLQFHDLVEQLAKLEHKIRAYYHGYLDLNS 120

Query: 121 ETLAWMMAVDASFLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLEN 180
           ETLAWMMA+DA FLLEFL+VYA +LK LRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLEN
Sbjct: 121 ETLAWMMAIDACFLLEFLNVYAQQLKILRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLEN 180

Query: 181 QIPLFVLRKMMEFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDFL 240
           QIPLFVLRKMMEFQFES+EQSDE LLSM+ G F ELSPFNTTD IP+IQISDCAHLLDFL
Sbjct: 181 QIPLFVLRKMMEFQFESTEQSDETLLSMLIGLFAELSPFNTTDEIPEIQISDCAHLLDFL 240

Query: 241 YQMITPKSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFL----KHLISFTKRAMQSQ 300
           YQMITPK KEPSEI E PNEIEQSPQI SYR++ AH SKQ L    ++LI FTKRAMQSQ
Sbjct: 241 YQMITPKLKEPSEITENPNEIEQSPQILSYRQQIAHRSKQCLLRSIENLIRFTKRAMQSQ 300

Query: 301 HVKTIARLPWTILSTIPPFAVLKQ---ILFFREQQEAKEETESQNPNSNPNANRPPLTEE 360
           HVKTIARLPW ILS+IPPFA+LKQ   ILFFREQ     ETE QNP+SNPNANRPPLTEE
Sbjct: 301 HVKTIARLPWAILSSIPPFAILKQPAEILFFREQ-----ETEPQNPDSNPNANRPPLTEE 360

Query: 361 ITIPSVSELFKAGIRFSPSHGGISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASS 420
           ITIPSVSELFKAGIRFSPSHGGISTITF+  TG LYLPT RLDVNTQATLRNLVAYEASS
Sbjct: 361 ITIPSVSELFKAGIRFSPSHGGISTITFDRNTGTLYLPTVRLDVNTQATLRNLVAYEASS 420

Query: 421 GLGALVFTRYTELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVL 480
           GLG LV TRYTELMNGIVDS+ED+RLLREGGVI+NHLKSDEEGAAMWNGMSKS+RLSRVL
Sbjct: 421 GLGPLVLTRYTELMNGIVDSDEDVRLLREGGVILNHLKSDEEGAAMWNGMSKSMRLSRVL 480

Query: 481 ELDKVIEEVNEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCF 540
           ELDKVIEEVNEYY+GLWRVKMGRFV  Y+YSSWPLLALMAVAW LFLIALQAFCSLFGCF
Sbjct: 481 ELDKVIEEVNEYYDGLWRVKMGRFVDAYIYSSWPLLALMAVAWVLFLIALQAFCSLFGCF 540

BLAST of Lsi07G004320 vs. NCBI nr
Match: XP_023528846.1 (putative UPF0481 protein At3g02645 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 900.6 bits (2326), Expect = 6.6e-258
Identity = 468/546 (85.71%), Postives = 496/546 (90.84%), Query Frame = 0

Query: 1   MASNSSSSTFDELKWVIQIRRTLEEELDDDDGGIPVTIFKVPKLLMASDPHSYTPQLVAL 60
           MASNS SSTFDE  WVIQIRRTLEEELDDDD GIPV+IFKVPK LMASDP SYTPQLVAL
Sbjct: 1   MASNSFSSTFDECNWVIQIRRTLEEELDDDD-GIPVSIFKVPKSLMASDPDSYTPQLVAL 60

Query: 61  GPYHCWRPELREMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLNA 120
           GPYHCWRPEL EM+R KLS+AKRAKKFSP +LQFHDLVEQL+KL+ KIRA YHGYLDLN+
Sbjct: 61  GPYHCWRPELHEMERIKLSAAKRAKKFSPRDLQFHDLVEQLAKLEHKIRAYYHGYLDLNS 120

Query: 121 ETLAWMMAVDASFLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLEN 180
           ETLAWMMA+DA FLLEFL+VYA +LK LRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLEN
Sbjct: 121 ETLAWMMAIDACFLLEFLNVYAQQLKILRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLEN 180

Query: 181 QIPLFVLRKMMEFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDFL 240
           QIPLFVLRKMMEFQFES+EQSDE LLSM+ G F ELSPFNTTD IP+IQISDCAHLLDFL
Sbjct: 181 QIPLFVLRKMMEFQFESTEQSDETLLSMLIGLFAELSPFNTTDEIPEIQISDCAHLLDFL 240

Query: 241 YQMITPKSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFL----KHLISFTKRAMQSQ 300
           YQMITPK KEPSEI E PNEIEQSPQI SYR++ AH SKQ L    ++LI FTKRAMQSQ
Sbjct: 241 YQMITPKLKEPSEITENPNEIEQSPQILSYRQQVAHRSKQCLLRSIENLIRFTKRAMQSQ 300

Query: 301 HVKTIARLPWTILSTIPPFAVLKQ---ILFFREQQEAKEETESQNPNSNPNANRPPLTEE 360
           HVKTIARLPW ILS+IPPFA+LKQ   ILFFREQ     ETE QNP+SNPNANRPPLTEE
Sbjct: 301 HVKTIARLPWAILSSIPPFAILKQPAEILFFREQ-----ETEPQNPDSNPNANRPPLTEE 360

Query: 361 ITIPSVSELFKAGIRFSPSHGGISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASS 420
           ITIPSVSELFKAGIRFSPSHGGISTITF+  TG LYLPT RLDVNTQATLRNLVAYEASS
Sbjct: 361 ITIPSVSELFKAGIRFSPSHGGISTITFDRNTGTLYLPTVRLDVNTQATLRNLVAYEASS 420

Query: 421 GLGALVFTRYTELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVL 480
           GLG LV TRYTELMNGIVDS+ED+RLLREGGVI+NHLKSDEEGAAMWNGMSKS+RLSRVL
Sbjct: 421 GLGPLVLTRYTELMNGIVDSDEDVRLLREGGVILNHLKSDEEGAAMWNGMSKSMRLSRVL 480

Query: 481 ELDKVIEEVNEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCF 540
           ELDKVIEEVNEYY+GLWRVKMGRFV+ Y+YSSWPLLALMAVAW LFLIALQAFCSLFGCF
Sbjct: 481 ELDKVIEEVNEYYDGLWRVKMGRFVEAYIYSSWPLLALMAVAWVLFLIALQAFCSLFGCF 540

BLAST of Lsi07G004320 vs. TAIR 10
Match: AT3G02645.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 480.3 bits (1235), Expect = 2.0e-135
Identity = 260/539 (48.24%), Postives = 357/539 (66.23%), Query Frame = 0

Query: 3   SNSSSSTFDELKWVIQIRRTLEEELDDDD-GGIPVTIFKVPKLLMASDPHSYTPQLVALG 62
           S++    FDE +WVI ++++L+ EL++ D   + V+IF VPK LM S P SYTP  V++G
Sbjct: 9   SSTEQHRFDETRWVINVQKSLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIG 68

Query: 63  PYHCWRPELREMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLNAE 122
           PYHC +PEL EM+R+KL  A++ +     + +FHDLVE+L  ++ KIRACYH Y+  N E
Sbjct: 69  PYHCLKPELHEMERYKLMIARKIRN-QYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGE 128

Query: 123 TLAWMMAVDASFLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAHNAVLRDIIMLENQ 182
           TL W+MAVD+SFL+EFL +Y     S R+V + ++ +         HN +LRDI+M+ENQ
Sbjct: 129 TLLWIMAVDSSFLIEFLKIY-----SFRKVETLINRV--------GHNEILRDIMMIENQ 188

Query: 183 IPLFVLRKMMEFQFESSEQSDEILLSMMNGFFTELSPF---NTTDGIPDIQISDCAHLLD 242
           IPLFVLRK +EFQ ES+E +D++LLS++ G   +LSP       D I   Q  +C H+LD
Sbjct: 189 IPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNHILD 248

Query: 243 FLYQMITPKSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFLKHLISFTKRAMQSQHV 302
           FLYQMI P+ +E         E+E+  + +   +   + + +F+  +    KR   S+  
Sbjct: 249 FLYQMIVPRIEE--------EELEEDDEENRADENGGNRAIRFMDEIKHQFKRVFASRPA 308

Query: 303 KTIARLPWTILSTIPPFAVLK---QILFFREQQEAKEETESQNPNSNPNANRPPLTEEIT 362
             I R PW I+S +P F  LK     LF R++ EA   T  Q   S  +  +PPL EE+T
Sbjct: 309 DLILRFPWRIISNLPGFMALKLSADYLFTRQENEA--TTTRQESVSILDIEKPPLVEELT 368

Query: 363 IPSVSELFKAGIRFSP-SHGGISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASSG 422
           IPSVS+L KAG+RF P +HG IST+TF++ +G  YLP   LD+NT+  LRNLVAYEA++ 
Sbjct: 369 IPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNT 428

Query: 423 LGALVFTRYTELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLSRVLE 482
            G LVFTRYTEL+NGI+DS ED+RLLRE GV+V+ LKSD+E A MWNGMSKS+RL++V  
Sbjct: 429 SGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSKSVRLTKVGF 488

Query: 483 LDKVIEEVNEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCF 534
           LDK IE+VN YY G W+VK+GR V+ YVY SW +LA +A    L L++LQ F  +F  F
Sbjct: 489 LDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFLAAVLLLMLVSLQLFSLVFSSF 523

BLAST of Lsi07G004320 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 133.7 bits (335), Expect = 4.6e-31
Identity = 134/527 (25.43%), Postives = 230/527 (43.64%), Query Frame = 0

Query: 15  WVIQIRRTLEEELDDDDGGI--PVTIFKVPKLLMASDPHSYTPQLVALGPYHCWRPELRE 74
           WVI I   LE+   DDD  +   + I++VP  L  +D  SY PQ V+LGPYH  +  LR 
Sbjct: 80  WVISITDKLEQAHRDDDTTLWGKLCIYRVPYYLQENDNKSYFPQTVSLGPYHHGKKRLRS 139

Query: 75  MDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLNAETLAWMMAVDAS 134
           MDR K  +  R  K +   ++ +  ++ + +L++K RACY G L L++     M+ +D  
Sbjct: 140 MDRHKWRAVNRVLKRTNQGIKMY--IDAMRELEEKARACYEGPLSLSSNEFIEMLVLDGC 199

Query: 135 FLLEFLHVYAGELKSLRRVS-SRMSHLMDYAGTKPAHNAVLRDIIMLENQIPLFVLRKMM 194
           F+LE   ++ G ++    +  +R   +    G+    +++ RD++MLENQ+PLFVL +++
Sbjct: 200 FVLE---LFRGAVEGFTELGYARNDPVFAMRGSM---HSIQRDMVMLENQLPLFVLNRLL 259

Query: 195 EFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDFLYQMITPKSKEP 254
           E Q  +  Q+  ++  +   FF  L P   TD                      P +K  
Sbjct: 260 ELQLGTRNQTG-LVAQLAIRFFDPLMP---TD---------------------EPLTKSG 319

Query: 255 SEIIEIPNEIEQSPQISSYRKRFAHISKQFLKHLISFTKRAMQSQHVKTIARLPWTILST 314
              +E  N + +        K F   +     H +   +R++     K   RL      T
Sbjct: 320 QSKLE--NSLARD-------KSFDPFADMGELHCLDVFRRSLLRSSPKPEPRL------T 379

Query: 315 IPPFAVLKQILFFREQQEAKEETESQNPNSNPNANRPPLTEEITIPSVSELFKAGIRFSP 374
              ++   ++   R QQ                           I  V+EL +AGI+F  
Sbjct: 380 RKRWSRNTRVADKRRQQ--------------------------LIHCVTELKEAGIKFRR 439

Query: 375 SHGGISTITF---NAKTGMLYLPTARLDVNTQATLRNLVAYEASSGLGALVFTRYTELMN 434
                 T  F     K G L +P   +   T++   NL+A+E      +   T Y   M+
Sbjct: 440 R----KTDRFWDMQFKNGYLEIPRLLIHDGTKSLFLNLIAFEQCHIDSSNDITSYIIFMD 499

Query: 435 GIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRL-SRVLELDKVIEEVNEYYN 494
            ++DS+ED+  L   G+I + L SD E A ++N + + +   +    L ++  EVN YY+
Sbjct: 500 NLIDSHEDVSYLHYCGIIEHWLGSDSEVADLFNRLCQEVVFDTEDSYLSRLSIEVNRYYD 528

Query: 495 GLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCFR 535
             W         +Y  + W +++  A    L L   Q+F +++  ++
Sbjct: 560 HKWNAWRATLKHKYFNNPWAIVSFCAAVILLVLTFSQSFYAVYAYYK 528

BLAST of Lsi07G004320 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 131.3 bits (329), Expect = 2.3e-30
Identity = 128/532 (24.06%), Postives = 215/532 (40.41%), Query Frame = 0

Query: 15  WVIQIRRTLEEELDDDDGGI--PVTIFKVPKLLMASDPHSYTPQLVALGPYHCWRPELRE 74
           WVI IR  LE+   DDD  I   + I++VP  L  +D  SY PQ V+LGPYH  +  LR 
Sbjct: 90  WVISIRDKLEQADRDDDTTIWGKLCIYRVPHYLQENDKKSYFPQTVSLGPYHHGKKRLRP 149

Query: 75  MDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLNAETLAWMMAVDAS 134
           M+R K  +  +  K     ++ +     + +L++K RACY G + L+      M+ +D  
Sbjct: 150 MERHKWRALNKVLKRLKQRIEMY--TNAMRELEEKARACYEGPISLSRNEFTEMLVLDGC 209

Query: 135 FLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAH------NAVLRDIIMLENQIPLFV 194
           F+LE   ++ G ++    +         YA   P        +++ RD+IMLENQ+PLFV
Sbjct: 210 FVLE---LFRGTVEGFTEIG--------YARNDPVFAMRGLMHSIQRDMIMLENQLPLFV 269

Query: 195 LRKMMEFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDFLYQMITP 254
           L +++E Q  +  Q+  I+  +   FF  L P       PD                   
Sbjct: 270 LDRLLELQLGTQNQTG-IVAHVAVKFFDPLMPTGEALTKPD------------------- 329

Query: 255 KSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFLKHLISFTKRAMQSQHVKTIARLPW 314
                           QS  ++   K    +  +   H +   +R++             
Sbjct: 330 ----------------QSKLMNWLEKSLDTLGDKGELHCLDVFRRSLL------------ 389

Query: 315 TILSTIPPFAVLKQILFFREQQEAKEETESQNPNSNPNANRPPLTEEITIPSVSELFKAG 374
                               Q      T S       N       ++  +  V+EL +AG
Sbjct: 390 --------------------QSSPTPNTRSLLKRLTRNTRVVDKRQQQLVHCVTELREAG 449

Query: 375 IRFSPSHGGISTITF---NAKTGMLYLPTARLDVNTQATLRNLVAYEASSGLGALVFTRY 434
           ++F        T  F     K G L +P   +   T++   NL+A+E      +   T Y
Sbjct: 450 VKFRKR----KTDRFWDIEFKNGYLEIPKLLIHDGTKSLFSNLIAFEQCHIESSNHITSY 509

Query: 435 TELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLS-RVLELDKVIEEV 494
              M+ +++S+ED+  L   G+I + L SD E A ++N + + +    +   L ++  +V
Sbjct: 510 IIFMDNLINSSEDVSYLHYCGIIEHWLGSDSEVADLFNRLCQEVVFDPKDSHLSRLSGDV 536

Query: 495 NEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCFR 535
           N YYN  W V       +Y  + W   +  A    L L   Q+F +++  ++
Sbjct: 570 NRYYNRKWNVLKATLTHKYFNNPWAYFSFSAAVILLLLTLCQSFYAVYAYYK 536

BLAST of Lsi07G004320 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 129.4 bits (324), Expect = 8.6e-30
Identity = 125/534 (23.41%), Postives = 228/534 (42.70%), Query Frame = 0

Query: 14  KWVIQIRRTLEEEL--DDDDGGIPVTIFKVPKLLMASDPHSYTPQLVALGPYHCWRPELR 73
           +WVI I+  +E+ L  D  +    + I++VP  L  +D  SY PQ V++GPYH  +  LR
Sbjct: 66  EWVISIKDKMEKALSYDATNSWDKLCIYRVPFYLQENDKKSYLPQTVSIGPYHHGKVHLR 125

Query: 74  EMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDL-NAETLAWMMAVD 133
            M+R K  +       +  N++ +  ++ + +L+++ RACY G +D+ N+     M+ +D
Sbjct: 126 PMERHKWRAVNMIMARTKHNIEMY--IDAMKELEEEARACYQGPIDMKNSNEFTEMLVLD 185

Query: 134 ASFLLEFLHVYAGELKSLRRVSSRMSHLMDYAGTKPAH------NAVLRDIIMLENQIPL 193
             F+LE   ++ G ++  +++         YA   P        +++ RD+IMLENQ+PL
Sbjct: 186 GCFVLE---LFKGTIQGFQKIG--------YARNDPVFAKRGLMHSIQRDMIMLENQLPL 245

Query: 194 FVLRKMMEFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDFLYQMI 253
           FVL +++  Q  +  Q+  I+  +   FF  L P +                       +
Sbjct: 246 FVLDRLLGLQTGTPNQTG-IVAEVAVRFFKTLMPTSE----------------------V 305

Query: 254 TPKSKEPSEIIEIPNEIEQSPQISSYRKRFAHISKQFLKHLISFTKRAMQSQHVKTIARL 313
             KS+   +  E  +E+  +  +                H +    R++      T    
Sbjct: 306 LTKSERSLDSQEKSDELGDNGGL----------------HCLDVFHRSLIQSSETTNQGT 365

Query: 314 PWTILSTIPPFAVLKQILFFREQQEAKEETESQNPNSNPNANRPPLTEEITIPSVSELFK 373
           P+  +S +             +QQ+                          I  V+EL  
Sbjct: 366 PYEDMSMV------------EKQQQ-------------------------LIHCVTELRG 425

Query: 374 AGIRFSPSH-GGISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASSGLGALVFTRY 433
           AG+ F     G +  I F  K G L +P   +   T++   NL+A+E      +   T Y
Sbjct: 426 AGVNFMRKETGQLWDIEF--KNGYLKIPKLLIHDGTKSLFSNLIAFEQCHTQSSNNITSY 485

Query: 434 TELMNGIVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLS-RVLELDKVIEEV 493
              M+ +++S++D+  L   G+I + L SD E A ++N + K +    +   L ++  EV
Sbjct: 486 IIFMDNLINSSQDVSYLHHDGIIEHWLGSDSEVADLFNRLCKEVIFDPKDGYLSQLSREV 508

Query: 494 NEYYNGLWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCFRAL 537
           N YY+  W        ++Y  + W   +  A    LFL   Q+F +++  ++ L
Sbjct: 546 NRYYSRKWNSLKATLRQKYFNNPWAYFSFSAAVILLFLTFFQSFFAVYAYYKPL 508

BLAST of Lsi07G004320 vs. TAIR 10
Match: AT3G50140.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 127.9 bits (320), Expect = 2.5e-29
Identity = 124/526 (23.57%), Postives = 219/526 (41.63%), Query Frame = 0

Query: 14  KWVIQIRRTLEEELDD--DDGGIPVTIFKVPKLLMASDPHSYTPQLVALGPYHCWRPELR 73
           +WVI I+  +E+ + D        + I++VP  L  SD +SY PQ V+LGPYH     LR
Sbjct: 89  EWVIWIKDKMEQVMRDAATTSWDKICIYRVPLSLKKSDKNSYFPQAVSLGPYHHGDEHLR 148

Query: 74  EMDRFKLSSAKRAKKFSPINLQFHDLVEQLSKLQQKIRACYHGYLDLNAETLAWMMAVDA 133
            MD  K  +     K +   ++ +  ++ + +L+++ RACY G + L++     M+ +D 
Sbjct: 149 PMDYHKWRAVNMVMKRTKQGIEMY--IDAMKELEERARACYEGPIGLSSNKFTQMLVLDG 208

Query: 134 SFLLEFLHVYAGELKSLRRVS-SRMSHLMDYAGTKPAHNAVLRDIIMLENQIPLFVLRKM 193
            F+L+   ++ G  +   ++   R   +    G+    +++ RD++MLENQ+PLFVL ++
Sbjct: 209 CFVLD---LFRGAYEGFSKLGYDRNDPVFAMRGSM---HSIRRDMLMLENQLPLFVLNRL 268

Query: 194 MEFQFESSEQSDEILLSMMNGFFTELSPFNTTDGIPDIQISDCAHLLDFLYQMITPKSKE 253
           +E Q  +  Q+  ++  +   FF  L P      +   +I +     +  +  I  K KE
Sbjct: 269 LELQLGTQYQTG-LVAQLAVRFFNPLMP----TYMSSTKIENSQENNNKFFNPIADKEKE 328

Query: 254 PSEIIEIPNEIEQSPQISSYRKRFAHISKQFLKHLISFTKRAMQSQHVKTIARLPWTILS 313
                                            H +   +R++    +K   RL  +  S
Sbjct: 329 E-------------------------------LHCLDVFRRSLLQPSLKPDPRLSRSRWS 388

Query: 314 TIPPFAVLKQILFFREQQEAKEETESQNPNSNPNANRPPLTEEITIPSVSELFKAGIRFS 373
             P  A  +Q                                +  +  V+EL +AGI+F 
Sbjct: 389 RKPLVADKRQ--------------------------------QQLLHCVTELREAGIKFK 448

Query: 374 PSHGG-ISTITFNAKTGMLYLPTARLDVNTQATLRNLVAYEASSGLGALVFTRYTELMNG 433
                    I F  K G L +P   +   T++   NL+AYE          T Y   M+ 
Sbjct: 449 RRKSDRFWDIQF--KNGCLEIPKLLIHDGTKSLFSNLIAYEQCHIDSTNDITSYIIFMDN 508

Query: 434 IVDSNEDLRLLREGGVIVNHLKSDEEGAAMWNGMSKSIRLS-RVLELDKVIEEVNEYYNG 493
           ++DS ED+R L    +I + L +D E A ++N + + +        L ++  +V+ YYN 
Sbjct: 509 LIDSAEDIRYLHYYDIIEHWLGNDSEVADVFNRLCQEVAFDLENTYLSELSNKVDRYYNR 536

Query: 494 LWRVKMGRFVKEYVYSSWPLLALMAVAWFLFLIALQAFCSLFGCFR 535
            W V       +Y  + W   +  A    L L   Q+F + +  F+
Sbjct: 569 KWNVLKATLKHKYFSNPWAYFSFFAAVILLLLTLFQSFFTSYPYFK 536

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0C8972.8e-13448.24Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KYJ32.2e-25985.69Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G006280 PE=4 SV=1[more]
A0A5A7TLR94.2e-25886.24Putative UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3BYU24.2e-25886.24putative UPF0481 protein At3g02645 OS=Cucumis melo OX=3656 GN=LOC103494677 PE=4 ... [more]
A0A6J1GW247.9e-25785.35putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111458007... [more]
A0A6J1IWY71.1e-25384.90putative UPF0481 protein At3g02645 OS=Cucurbita maxima OX=3661 GN=LOC111479236 P... [more]
Match NameE-valueIdentityDescription
XP_038890629.11.3e-27490.44putative UPF0481 protein At3g02645 [Benincasa hispida][more]
KAE8649063.14.6e-25985.69hypothetical protein Csa_014591 [Cucumis sativus][more]
XP_004152335.14.6e-25985.69putative UPF0481 protein At3g02645 [Cucumis sativus][more]
KAG6581946.15.1e-25885.71putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023528846.16.6e-25885.71putative UPF0481 protein At3g02645 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G02645.12.0e-13548.24Plant protein of unknown function (DUF247) [more]
AT3G50120.14.6e-3125.43Plant protein of unknown function (DUF247) [more]
AT3G50170.12.3e-3024.06Plant protein of unknown function (DUF247) [more]
AT3G50150.18.6e-3023.41Plant protein of unknown function (DUF247) [more]
AT3G50140.12.5e-2923.57Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 38..520
e-value: 2.6E-97
score: 326.6
IPR004158Protein of unknown function DUF247, plantPANTHERPTHR31549PROTEIN, PUTATIVE (DUF247)-RELATED-RELATEDcoord: 3..528
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 334..351
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 329..351
NoneNo IPR availablePANTHERPTHR31549:SF177BNACNNG05850D PROTEINcoord: 3..528

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G004320.1Lsi07G004320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane