Lsi07G004050 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi07G004050
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	aquaporin NIP6-1
Location	chr07: 4368172 .. 4374279 (+)
RNA-Seq Expression	Lsi07G004050
Synteny	Lsi07G004050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGTACAAAGTTAAATCAAATTGGTTTTAAGTCCCATTTTTGCTCTTCTTGAACTATTAAAATGTAAGTTTTAATATTTCATTCCTTTTTTTTTTAAAAAAAAATATGGTTCTGTTTTTATATAAATATATTTATAATTTTATTATACATTGTTTGACAAATGGATAACATGACCTTAAGTATGTGTATTAACATGCTAATGTAGACACATGTAATTATATAATCACATAGCTATATTGGCTTAAACGAGTTTAGGTCAATGTGACATTAAAGGCACATCTAGGACCATTAATGGTTGTTTTTTTTTTTTTTTTTTTTAATTTAAGAATTTAAATAAGAATCAAAATAAAATGTAGCTGAAAGAATTAAACCAATAAATATAATTCATAATTGATACAAGTTCATACAATCACCAATAATAGTCACGTTCATCCAAAGGATTTTGTTTAGAATCACAACAATTTCCATCATATCATCATGTTGATTTAAGAGGATCAGATCACAAAAACTTATATATATGCAAATGATCTTAGAAGATCCATATTATTGGAATCCAATAATATATGCAAAAGTGGCATTTGAGAGAGAGAAAGACAGAACAACGAATATTATAAAAATAATAAAAAAATAATAAGAAAAATAAAAATATTTAGTTGCATCTAGGGCCCACTTACATGTCCCATTACAAGCGGCCAGAAAGCAATTTTTCTTTTGAATAATTGTAACGAGTATCAATTTTAAGAATAATAACTAAGTATATCGCAATATTTTTAAAAAATTGCAAATATAATAAAGTTTATCCATGTAGACTTCTATCGCTGATAGACTCTTATGGTCTATCGATGATAAACCACCTAAACTATAAAAGTCTATCACTGATAGAATTTGCTATATTCGTAATTTTTTTAAAATATTGTTATATATACTAATATTTTGAATCTAATTACTATATTAGCAACCGTCCATTTTCTTTTCTTTCGAAAAGATACCTTTTTATATTATAAAGAGTAAAACTTTTGATGAAAGAGGGTCCAATATTATTTACAACCACTGACTTATGTTCTTTTTCTATCTTTTTCTCTTGGGATTCCCTTAAGTCAGCCAAAATTTTCTTCTATAAATAAGAAAGCATTCAGCATAGTTCACTCTCCAACCACAAAAAGACAAACCCCAAAACCAAAAACATAACAAACTTAACCTTGTTTTTTCTTTAGCTTCCCTTTCTTTTTAAAAGTTATTTGGTGTTATGGACACAGAGGAAGCTCCATCGACACCGGTGACGCCGGGGACGCCCGGGGCTCCGCTGTTTGGACGAGTGAAGGAAAATCAGCATGGAAGTGAGAATGGTAAAAGATCTCTTCTCAAAAGCTGCATCTCTTTCAGTGTTGATGATAATTGGGGCACTGAAGAAGGAAGCTTATCTAAATTAGTCTCTTCTTGTTCATTGCCATACCCTCCTGTCTCACTTGCAAGAAAGGTATACCCAATATATATATAGAAACACCCTTTTAATTACTAAAGGCCCCTTTGATAACGTTTTTGTTCTAAACTTTACACTAACCCATATCTTATAAAAAAAAAATACTTAGATGCAATTGTCCAACAACAGTTTACCACACGACATTCTTTTTTCTTTATTATTATTTTTTTAAATTAACTTTTCTTGTTGTTGTGTAATTGGAGTAGACTGCACAAAAGATGAGTATAGGTCACGATGCATTCCAAATATTACTCTATTTTCGGTGGCACTTTATCTTCCAACTCCTCTAACTTTGCATTATGGTCAGAACAATAATAGAGAAGGAAGAAGAAAGCAAGACTCTCTGTAATGAGTCATGTATTTAAAAGAGAATACATTAAAACAAATGATATCCTTTAATTAATTTTGTCTTTCTAAATCTTTTGAAGATCTACCGACGTTTTCTTGCATAGGCAGAGAAAAAAAATTCTTGTAGTGGATTATATATATAAAAGTTGCCTCCACTTTTGCTGATAGGTGGGAGCGGAGTTCATAGGTACTTTGATACTCATCTTTGCCGGAACTGCCACGGCTATCGTGAACCAAAAGACTGAAGGAACCGAAACACTGATTGGTTTGGCGGCTTCCACCGGCCTTGCGGTGATGGTCGTGATCTTGTCGACGGGCCATATCTCCGGAGCCCATCTGAACCCGGCTATCACTATTGCTTTTGCTGCATTGAAGCAGTTTCCATGGAAACACGTATGTAACTAACCACCATGATCTTTGTACTTTCATCCATTGTGATTTAATAAGAATCTCTCAGGCATTCAATCTCTTGATATATATATTTAATAATTTGGCAGGTGCCAGTGTATATTGGAGCTCAAATGGTGGCTTCTTTGTGTTCTTCTTTTGCATTGAAATGGATCTTTGATCCCATAATGGGAGGAGGAGCCACTGTTCCTTCATGTGGATATGCTCAAGCTTTTGCTCTTGAGTTTATCATTAGCTTCAACCTCATGTTTGTCATCACCGCGGTCGCCACTGACACTCGGGCTGTGAGTTCCTCTTCTTTTCTGTTCTTTTTTTCATTAAGAATGAGAAAAAAAAAATCATCAATAAAATATAGATTATATTGCATTTTTGGCATTATAATCTTTGGAATCTAAATCACACATAAGTTGATAAACATTAATTTTTTAATTGGGTTTTAAGTTATTATTTTAAGAAAAATACTTAGTGCATCTTTTGTTGCATCTATCTTTATGGATTCTATCAAAATTTGTCACATGACAAATTCTTTATATATATATATTTTTAAATATTTTAATCCATTATTTGTATGTGTGAGGAAAGGTCTCATTTTAAAATGTTATTAAAAGTTACCACATGACAGTTTTTTATTTGACGAGTTAAACTTAAGTATTTTTCTAAATAAATTATCTTTTACGCAAATATCAATTTACACTTCTAAATTTTGGGTTGTATCAATTAAAACCCTGAATAATTATATTAGTTTAAATTTTAGACTTTCATATAGCAGTTTACAACCTCCTACAGATTTCATTCGTCCAATATCCTATGAAACTTATGATTTGAGTCAATTAAACTCCTAATTTTTCATAAGTCAATCAATCTAAACATTTCATTGCGATTACTTTTGAAAATTATTCATGCATTGATTCTCAAATATGTGCAACTTTTTTCAAATGTCGATTTTACAAAATGGACGATTAAATACATGGATGATTCCTAAAGGAATTTATGACTAAGAGTCTAAATTGAATGGACGATTAAATGCATGGACTGTTCTTAAAGAAAATTATTACTAAGAGTCTAAATTGATCAATTTATGAATATTTAGGGGTCTAATTGATACAATTATAAGTTTTCCAAGAGATTTTCTTAAATCGACCATAATGGAGGGTGTAAATTGATATACTTACAAAAATTCAGAGTTTAAATTGATACAATTATTAATTTAAAGTTTAAATTGATACAACCCAAAAGTTTAAGGTATAAATTTATATTTTTCCTTATCTTTTTAGTCTTGTTAAATATTCAAATGATGAACTTAATGTAAGTTCATGTGTGTTATAATTGTGAAAAATTGAGGATAGTTCAACTCCCAGAAGTATTTATCATCGATTAAGAAGTTGAATGTTCTAAATAAAAATAATAAAAATAATAAAATAAAATTGAAGGGAGATGACGTGGAATTAGAAATGCAGGTGGGAGAGTTGGCGGGAATCGCGGTAGGAGCAACAGTTATGCTCAATGTACTCATCGCCGGGTATGTCCAAAACTATCACAATAAATTACATCTTTACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTAATTAATTAATTTTATTATTAATTTTTTTTTTCTTTAGAAATTATAAATTTGGTTTCATAGTTCTAAAATTTTTAATTAGACTAAATTTTCTTAATTAATATAAGTTGAAAAATGGAGAATTTTTAAAAATAGAAAAATTAGAGAAATTATTTACACAAAATGACAAAATTTTTAGATAGTTGTGATAGAAATGATAGTCTATCAATATCTATCCGTGTTTTTTTTTTTTTTTTTTGCTATTTTATGTAAATAGTTCGACATTTTTTCTAATTGTAAAAATTTTCCTTGGAAAAACTCGGAGACTATAGCTTAGAGACTAAATTGTCTTTCTCTTCTTTTGCATAAAATCAAATCTATTCTATCAACTTGACCTAATTAAATCAAGTCTCCACCTACTTGAAGTTTGGAGCCCAAACACCCCTAAATTGTTACAATAACTTTTGTCATGTTCAAAATTAAAACACTGTCTTCAAGGACTCAAAATTAATTTAATATTTAATTTTACACTTTTCAATACAATTTTTATTTTATCAAAATCAGTTTTGAATCATTAAAAGCATGTTTCATAGTGCTTATAAAAATGATAAAACTGAATTTTAACCATTTTAGAATTACTTTCAAACATGTCCTTAATCGTTACAAATCAAAGTTTAATGACCCAAAGTGATTTTCACCCATACAAATATTTGAAAACGTATTCACTTCATATATGATACAAACTCAAACTGGTAATGACTTGTCCAACTCAAAATAATAGATCGGTCATATTCCTGTATAATGCAGTTAGAACTTGAGTTAAACAGATGTCAACAAAGTGGCTACTGTTTTAGAAAGGAAGCAGTTTGTAATTTAGAGCATCTTATGAACAACTTCACCTTTCACTAACCAACAGAAAAATTAAAAGCGGAAAATTTTCATAGATAGAAAAAATGTCAAACTATTTACAGAAAATAGCAAAAAGAAATACGGATAGACATTGATAGACTTCTATCAGCATCTATCAGTGATAGACTTCTATCATTTCTATCACCAATAGACCCGGATAAACTTTTATCAGCGTCTATCATAACTATATAAAAAAATTTCTATTTCGTGTAAATAGTTTTTCTTATTTTATTATTTTTAAAAGTTTTCCCTTTTAATTATTTAACAGACAAACAACTGGAGCTTCAATGAATCCAGTAAGAACACTGGGGCCAGCCATTGCTGCAAACAACTTTAGAGCCATATGGATTTACCTCACAGCTCCCATACTAGGGACATTGTGTGGAGCTGGAATCTACACTGCAGTTAAGTTGCCCGACAAAGATCGCGATTCACGATTGCCTTCTACTGCAGGAAGCTTTCGAAGATGA

mRNA sequence

ATGAAATTATTTGGTGTTATGGACACAGAGGAAGCTCCATCGACACCGGTGACGCCGGGGACGCCCGGGGCTCCGCTGTTTGGACGAGTGAAGGAAAATCAGCATGGAAGTGAGAATGGTAAAAGATCTCTTCTCAAAAGCTGCATCTCTTTCAGTGTTGATGATAATTGGGGCACTGAAGAAGGAAGCTTATCTAAATTAGTCTCTTCTTGTTCATTGCCATACCCTCCTGTCTCACTTGCAAGAAAGACTGAAGGAACCGAAACACTGATTGGTTTGGCGGCTTCCACCGGCCTTGCGGTGATGGTCGTGATCTTGTCGACGGGCCATATCTCCGGAGCCCATCTGAACCCGGCTATCACTATTGCTTTTGCTGCATTGAAGCAGTTTCCATGGAAACACGTGCCAGTGTATATTGGAGCTCAAATGGTGGCTTCTTTGTGTTCTTCTTTTGCATTGAAATGGATCTTTGATCCCATAATGGGAGGAGGAGCCACTGTTCCTTCATGTGGATATGCTCAAGCTTTTGCTCTTGAGTTTATCATTAGCTTCAACCTCATGTTTGTCATCACCGCGGTCGCCACTGACACTCGGGCTGGAGATGACGTGGAATTAGAAATGCAGGTGGGAGAGTTGGCGGGAATCGCGGTAGGAGCAACAGTTATGCTCAATGTACTCATCGCCGGAGCCATATGGATTTACCTCACAGCTCCCATACTAGGGACATTGTGTGGAGCTGGAATCTACACTGCAGTTAAGTTGCCCGACAAAGATCGCGATTCACGATTGCCTTCTACTGCAGGAAGCTTTCGAAGATGA

Coding sequence (CDS)

ATGAAATTATTTGGTGTTATGGACACAGAGGAAGCTCCATCGACACCGGTGACGCCGGGGACGCCCGGGGCTCCGCTGTTTGGACGAGTGAAGGAAAATCAGCATGGAAGTGAGAATGGTAAAAGATCTCTTCTCAAAAGCTGCATCTCTTTCAGTGTTGATGATAATTGGGGCACTGAAGAAGGAAGCTTATCTAAATTAGTCTCTTCTTGTTCATTGCCATACCCTCCTGTCTCACTTGCAAGAAAGACTGAAGGAACCGAAACACTGATTGGTTTGGCGGCTTCCACCGGCCTTGCGGTGATGGTCGTGATCTTGTCGACGGGCCATATCTCCGGAGCCCATCTGAACCCGGCTATCACTATTGCTTTTGCTGCATTGAAGCAGTTTCCATGGAAACACGTGCCAGTGTATATTGGAGCTCAAATGGTGGCTTCTTTGTGTTCTTCTTTTGCATTGAAATGGATCTTTGATCCCATAATGGGAGGAGGAGCCACTGTTCCTTCATGTGGATATGCTCAAGCTTTTGCTCTTGAGTTTATCATTAGCTTCAACCTCATGTTTGTCATCACCGCGGTCGCCACTGACACTCGGGCTGGAGATGACGTGGAATTAGAAATGCAGGTGGGAGAGTTGGCGGGAATCGCGGTAGGAGCAACAGTTATGCTCAATGTACTCATCGCCGGAGCCATATGGATTTACCTCACAGCTCCCATACTAGGGACATTGTGTGGAGCTGGAATCTACACTGCAGTTAAGTTGCCCGACAAAGATCGCGATTCACGATTGCCTTCTACTGCAGGAAGCTTTCGAAGATGA

Protein sequence

MKLFGVMDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFSVDDNWGTEEGSLSKLVSSCSLPYPPVSLARKTEGTETLIGLAASTGLAVMVVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPIMGGGATVPSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVGATVMLNVLIAGAIWIYLTAPILGTLCGAGIYTAVKLPDKDRDSRLPSTAGSFRR

Homology

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BLAST of Lsi07G004050 vs. ExPASy Swiss-Prot
Match: Q9SAI4 (Aquaporin NIP6-1 OS=Arabidopsis thaliana OX=3702 GN=NIP6-1 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 2.5e-83
Identity = 185/319 (57.99%), Postives = 207/319 (64.89%), Query Frame = 0


Query: 7   MDTEEAPSTP----VTPGTPGAPLFG--RVKENQHGSENGKRSLLKSCISFSVDDNWGTE 66
           MD EE PSTP     TPGTPGAPLFG    K N H      +SLLKSC  FSVD+ W  E
Sbjct: 1   MDHEEIPSTPSTPATTPGTPGAPLFGGFEGKRNGHNGRYTPKSLLKSCKCFSVDNEWALE 60

Query: 67  EGSLSKLVSSCSLPYPPVSLAR------------------------KTEGTETLIGLAAS 126
           +G L  +  +CSLP P VSL R                        KT+G ETLIG AAS
Sbjct: 61  DGRLPPV--TCSLPPPNVSLYRKLGAEFVGTLILIFAGTATAIVNQKTDGAETLIGCAAS 120

Query: 127 TGLAVMVVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWI 186
            GLAVM+VILSTGHISGAHLNPA+TIAFAALK FPWKHVPVYIGAQ++AS+ ++FALK +
Sbjct: 121 AGLAVMIVILSTGHISGAHLNPAVTIAFAALKHFPWKHVPVYIGAQVMASVSAAFALKAV 180

Query: 187 FDPIMGGGATVPSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIA 246
           F+P M GG TVP+ G +QAFALEFIISFNLMFV+TAVATDTRA         VGELAGIA
Sbjct: 181 FEPTMSGGVTVPTVGLSQAFALEFIISFNLMFVVTAVATDTRA---------VGELAGIA 240

Query: 247 VGATVMLNVLIAG-----------------------AIWIYLTAPILGTLCGAGIYTAVK 273
           VGATVMLN+LIAG                       AIW+YLTAPILG L GAG YT VK
Sbjct: 241 VGATVMLNILIAGPATSASMNPVRTLGPAIAANNYRAIWVYLTAPILGALIGAGTYTIVK 300

[Close]

BLAST of Lsi07G004050 vs. ExPASy Swiss-Prot
Match: Q9SV84 (Probable aquaporin NIP5-1 OS=Arabidopsis thaliana OX=3702 GN=NIP5-1 PE=2 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 4.2e-62
Identity = 145/308 (47.08%), Postives = 176/308 (57.14%), Query Frame = 0


Query: 12  APSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFSVDDNWGTEEGSLSKLVSSC 71
           AP TP TPGTPG PL   ++ +    ++ K +    C+   +   WG  +   +      
Sbjct: 15  APPTPGTPGTPGGPLITGMRVDSMSFDHRKPTPRCKCLPV-MGSTWGQHDTCFT------ 74

Query: 72  SLPYPPVSLARKT------------------------EGTETLIGLAASTGLAVMVVILS 131
             P P VSL RK                         +G ETLIG AA  GLAVM++ILS
Sbjct: 75  DFPSPDVSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILS 134

Query: 132 TGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPIMGGGATV 191
           TGHISGAHLNP++TIAFAAL+ FPW HVP YI AQ+ AS+C+SFALK +F P M GG T+
Sbjct: 135 TGHISGAHLNPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTI 194

Query: 192 PSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVGATVMLNVLI 251
           PS    QAFALEFII+F L+FV+TAVATDTRA         VGELAGIAVGATVMLN+L+
Sbjct: 195 PSVSLGQAFALEFIITFILLFVVTAVATDTRA---------VGELAGIAVGATVMLNILV 254

Query: 252 AG-----------------------AIWIYLTAPILGTLCGAGIYTAVKLPDKDRDSRLP 273
           AG                       ++W+YL AP LG + GA +YT VKL D   D   P
Sbjct: 255 AGPSTGGSMNPVRTLGPAVASGNYRSLWVYLVAPTLGAISGAAVYTGVKLNDSVTDP--P 304

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BLAST of Lsi07G004050 vs. ExPASy Swiss-Prot
Match: Q9ATN1 (Aquaporin NIP3-1 OS=Zea mays OX=4577 GN=NIP3-1 PE=2 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.8e-52
Identity = 139/309 (44.98%), Postives = 167/309 (54.05%), Query Frame = 0


Query: 14  STPVTPGTPGAPLFGRVKENQHGSENGKRSLLK-SCISFSVDDNWGTEEGSLSKLVSSCS 73
           S P TPGTP APLF             ++S+ +  C+     + WG     ++       
Sbjct: 11  SAPATPGTP-APLFSSGGPRVDSLSYERKSMPRCKCLPLPAVEGWG-----VATHTCVVE 70

Query: 74  LPYPPVSLARKT----EGTETLI--------------------GLAASTGLAVMVVILST 133
           +P P VSL RK      GT  LI                    G AA  GLAV  VILST
Sbjct: 71  IPAPDVSLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACAGLAVATVILST 130

Query: 134 GHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPIMGGGATVP 193
           GHISGAHLNP++TIAFAAL+ FPW  VP Y+  Q +AS+C++FALK +F P + GG TVP
Sbjct: 131 GHISGAHLNPSLTIAFAALRHFPWLQVPAYVAVQALASVCAAFALKGVFHPFLSGGVTVP 190

Query: 194 --SCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVGATVMLNVL 253
             +   AQAF  EFIISFNL+FV+TAVATDTRA         VGELAGIAVGA V LN+L
Sbjct: 191 DATVSTAQAFFTEFIISFNLLFVVTAVATDTRA---------VGELAGIAVGAAVTLNIL 250

Query: 254 IAG-----------------------AIWIYLTAPILGTLCGAGIYTAVKLPDKDRDSRL 273
           +AG                        +WIYL AP LG L GA +Y AVKL  +D +   
Sbjct: 251 VAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLLAPTLGALAGASVYKAVKL--RDENGET 302

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BLAST of Lsi07G004050 vs. ExPASy Swiss-Prot
Match: Q0IWF3 (Aquaporin NIP3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-1 PE=2 SV=2)

HSP 1 Score: 206.5 bits (524), Expect = 3.9e-52
Identity = 140/317 (44.16%), Postives = 168/317 (53.00%), Query Frame = 0


Query: 5   GVMDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFSVDDNWGTEEGSL 64
           G+       S P TPGTP APLF   + +    E       K C+  +V + W       
Sbjct: 13  GMSSPVNGASAPATPGTP-APLFAGPRVDSLSYERKSMPRCK-CLPAAVAEAWAP----- 72

Query: 65  SKLVSSCSLPYPPVSLARKT----EGTETLI--------------------GLAASTGLA 124
           S       +P P VSL RK      GT  LI                    G AA  GLA
Sbjct: 73  SAHGCVVEIPAPDVSLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACAGLA 132

Query: 125 VMVVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPI 184
           V  +ILSTGHISGAHLNP++TIAFAAL+ FPW  VP Y+  Q++ S+C+ FALK +F P 
Sbjct: 133 VTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQVPAYVAVQVLGSICAGFALKGVFHPF 192

Query: 185 MGGGATV--PSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVG 244
           + GG TV  P+   AQAF  EFII+FNL+FV+TAVATDTRA         VGELAGIAVG
Sbjct: 193 LSGGVTVPDPTISTAQAFFTEFIITFNLLFVVTAVATDTRA---------VGELAGIAVG 252

Query: 245 ATVMLNVLIAG-----------------------AIWIYLTAPILGTLCGAGIYTAVKLP 273
           A V LN+LIAG                        +WIYL AP LG + GAG+YTAVKL 
Sbjct: 253 AAVTLNILIAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVYTAVKL- 311

[Close]

BLAST of Lsi07G004050 vs. ExPASy Swiss-Prot
Match: Q0DK16 (Aquaporin NIP1-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-3 PE=3 SV=2)

HSP 1 Score: 151.4 bits (381), Expect = 1.5e-35
Identity = 89/209 (42.58%), Postives = 121/209 (57.89%), Query Frame = 0


Query: 78  VSLARKTEGTETLIGLAASTGLAVMVVILSTGHISGAHLNPAITIAFAALKQFPWKHVPV 137
           V++ ++T GT T  G+  + GLAVMV++ S GHISGAHLNPA+T+AFA   +FPW+ VP 
Sbjct: 74  VAVNKRTGGTVTFPGICITWGLAVMVMVYSVGHISGAHLNPAVTLAFATCGRFPWRRVPA 133

Query: 138 YIGAQMVASLCSSFALKWIFDPIMGGGA------TVPSCGYAQAFALEFIISFNLMFVIT 197
           Y  AQ+  S  +S AL+ +F     GGA      T P+    Q+ A+EFII+F LMFV++
Sbjct: 134 YAAAQVAGSAAASAALRALF-----GGAPEHFFGTAPAGSDVQSLAMEFIITFYLMFVVS 193

Query: 198 AVATDTRAGDDVELEMQVGELAGIAVGATVMLNVLIAG---------------------- 257
            VATD RA         +GELAG+AVGATV++NVL AG                      
Sbjct: 194 GVATDNRA---------IGELAGLAVGATVLVNVLFAGPISGASMNPARTIGPAIILGRY 253

[Close]

BLAST of Lsi07G004050 vs. ExPASy TrEMBL
Match: A0A5D3E2S7 (Aquaporin NIP6-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002260 PE=3 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 4.4e-123
Identity = 244/313 (77.96%), Postives = 251/313 (80.19%), Query Frame = 0


Query: 7   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFSVDDNWGTEEGSLSK 66
           MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKS I F+VDD+WG EEG LSK
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLSK 60

Query: 67  LVSSCSLPYPPVSLAR------------------------KTEGTETLIGLAASTGLAVM 126
           +VSSCSLPYPPVSLAR                        KTEGTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAVM 120

Query: 127 VVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPIMG 186
           +VILSTGHISGAHLNPA+TIAFAALKQFPWKHVP+YIGAQMVASLCSSFALKWIFDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180

Query: 187 GGATVPSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVGATVM 246
           GGATVPSCGYAQAFALEFIISFNLMFV+TAVATDTRA         VGELAGIAVGATVM
Sbjct: 181 GGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRA---------VGELAGIAVGATVM 240

Query: 247 LNVLIAG-----------------------AIWIYLTAPILGTLCGAGIYTAVKLPDKDR 273
           LNVLIAG                       AIWIYLTAPILGTLCGAGIYTAVKLPDKD 
Sbjct: 241 LNVLIAGQTTGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDG 300

[Close]

BLAST of Lsi07G004050 vs. ExPASy TrEMBL
Match: A0A1S3BY36 (aquaporin NIP6-1 OS=Cucumis melo OX=3656 GN=LOC103494651 PE=3 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 4.4e-123
Identity = 244/313 (77.96%), Postives = 251/313 (80.19%), Query Frame = 0


Query: 7   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFSVDDNWGTEEGSLSK 66
           MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKS I F+VDD+WG EEG LSK
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLSK 60

Query: 67  LVSSCSLPYPPVSLAR------------------------KTEGTETLIGLAASTGLAVM 126
           +VSSCSLPYPPVSLAR                        KTEGTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAVM 120

Query: 127 VVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPIMG 186
           +VILSTGHISGAHLNPA+TIAFAALKQFPWKHVP+YIGAQMVASLCSSFALKWIFDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180

Query: 187 GGATVPSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVGATVM 246
           GGATVPSCGYAQAFALEFIISFNLMFV+TAVATDTRA         VGELAGIAVGATVM
Sbjct: 181 GGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRA---------VGELAGIAVGATVM 240

Query: 247 LNVLIAG-----------------------AIWIYLTAPILGTLCGAGIYTAVKLPDKDR 273
           LNVLIAG                       AIWIYLTAPILGTLCGAGIYTAVKLPDKD 
Sbjct: 241 LNVLIAGQTTGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDG 300

[Close]

BLAST of Lsi07G004050 vs. ExPASy TrEMBL
Match: A0A5A7TNN6 (Aquaporin NIP6-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002250 PE=3 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 5.8e-123
Identity = 243/313 (77.64%), Postives = 251/313 (80.19%), Query Frame = 0


Query: 7   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFSVDDNWGTEEGSLSK 66
           MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKS I F+VDD+WG EEG LSK
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLSK 60

Query: 67  LVSSCSLPYPPVSLAR------------------------KTEGTETLIGLAASTGLAVM 126
           +VSSCSLPYPPVSLAR                        KTEGTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAVM 120

Query: 127 VVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPIMG 186
           +VILSTGHISGAHLNPA+TIAFAALKQFPWKHVP+YIGAQMVASLCSSFALKWIFDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180

Query: 187 GGATVPSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVGATVM 246
           GGATVPSCGYAQAFALEFIISFNLMFV+TAVATDTRA         VGELAGIAVGATVM
Sbjct: 181 GGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRA---------VGELAGIAVGATVM 240

Query: 247 LNVLIAG-----------------------AIWIYLTAPILGTLCGAGIYTAVKLPDKDR 273
           LN+LIAG                       AIWIYLTAPILGTLCGAGIYTAVKLPDKD 
Sbjct: 241 LNILIAGQTTGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDG 300

[Close]

BLAST of Lsi07G004050 vs. ExPASy TrEMBL
Match: A0A0A0KX08 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007030 PE=3 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 5.8e-123
Identity = 242/313 (77.32%), Postives = 251/313 (80.19%), Query Frame = 0


Query: 7   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFSVDDNWGTEEGSLSK 66
           MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISF+VDDNWG+EEG L+K
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60

Query: 67  LVSSCSLPYPPVSLAR------------------------KTEGTETLIGLAASTGLAVM 126
           +VSSCSLPY PVSLAR                        KT GTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120

Query: 127 VVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPIMG 186
           +VILSTGHISGAHLNPA+TIAFAALKQFPWKHVP+YIGAQMVASLCSSFALKW FDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWTFDPIMG 180

Query: 187 GGATVPSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVGATVM 246
           GGAT+PSCGYAQAFALEFIISFNLMFV+TAVATDTRA         VGELAGIAVGATVM
Sbjct: 181 GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRA---------VGELAGIAVGATVM 240

Query: 247 LNVLIAG-----------------------AIWIYLTAPILGTLCGAGIYTAVKLPDKDR 273
           LNVLIAG                       AIWIYLTAPILGTLCGAGIYTAVKLPDKD 
Sbjct: 241 LNVLIAGQTTGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDG 300

[Close]

BLAST of Lsi07G004050 vs. ExPASy TrEMBL
Match: A0A6J1KEN1 (aquaporin NIP6-1 OS=Cucurbita maxima OX=3661 GN=LOC111492577 PE=3 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 7.9e-104
Identity = 217/302 (71.85%), Postives = 232/302 (76.82%), Query Frame = 0


Query: 7   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFSVDDNWGTEEGSLSK 66
           MDTEE PS PVTPGTPGAPLFG  K N  GSENGKRSLLKSCISF+VDD WGTEEGSLSK
Sbjct: 1   MDTEEVPS-PVTPGTPGAPLFGGHKGNHQGSENGKRSLLKSCISFNVDD-WGTEEGSLSK 60

Query: 67  L----------VSSCSLPYPPVSLA---RKTEGTETLIGLAASTGLAVMVVILSTGHISG 126
           +          + +  L +   + A   +K +GTETLIGLAASTG+AVMVVILSTGHISG
Sbjct: 61  VSLARKVGAEFIGTLMLIFAGTATAIVNQKIQGTETLIGLAASTGMAVMVVILSTGHISG 120

Query: 127 AHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPIMGGGATVPSCGYA 186
           AHLNP ITIAFAALK FPWK VPVYIGAQ+VASLC+SFALKWIFDP MGGG TVPSCGY+
Sbjct: 121 AHLNPGITIAFAALKHFPWKQVPVYIGAQIVASLCASFALKWIFDPFMGGGVTVPSCGYS 180

Query: 187 QAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVGATVMLNVLIAG---- 246
           QAFALEFIISFNLMFVITAVATDTRA         VGELAGI VGATVMLNVLIAG    
Sbjct: 181 QAFALEFIISFNLMFVITAVATDTRA---------VGELAGIVVGATVMLNVLIAGETTG 240

Query: 247 -------------------AIWIYLTAPILGTLCGAGIYTAVKLPDKDRDSRLPSTAGSF 273
                              AIWIYLTAPILGTLCGAGIYTA+KLPD+D D+RLPSTA SF
Sbjct: 241 ASMNPVRTLGPAIAANNFRAIWIYLTAPILGTLCGAGIYTAIKLPDQDGDARLPSTARSF 291

[Close]

BLAST of Lsi07G004050 vs. NCBI nr
Match: XP_038876197.1 (aquaporin NIP6-1 [Benincasa hispida])

HSP 1 Score: 454.5 bits (1168), Expect = 6.3e-124
Identity = 245/313 (78.27%), Postives = 252/313 (80.51%), Query Frame = 0


Query: 7   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFSVDDNWGTEEGSLSK 66
           MDTEE PSTPVTPGTPGAPLFGRVKEN+HGSENGKRSLLKSCISF+VDDNWGTEEG LSK
Sbjct: 1   MDTEETPSTPVTPGTPGAPLFGRVKENRHGSENGKRSLLKSCISFNVDDNWGTEEGGLSK 60

Query: 67  LVSSCSLPYPPVSLAR------------------------KTEGTETLIGLAASTGLAVM 126
           LVSSCSLPYPPVSLAR                        KTEGTE LIGLAASTGLAVM
Sbjct: 61  LVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTEMLIGLAASTGLAVM 120

Query: 127 VVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPIMG 186
           +VILSTGHISGAHLNPAITIAFAALKQFPWK+VPVYIGAQMVASLCSSFALKW+FDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAITIAFAALKQFPWKYVPVYIGAQMVASLCSSFALKWMFDPIMG 180

Query: 187 GGATVPSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVGATVM 246
           GGATVPSCGYAQAFALEFIISFNLMFV+TAVATDTRA         VGELAGIAVGATVM
Sbjct: 181 GGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRA---------VGELAGIAVGATVM 240

Query: 247 LNVLIAG-----------------------AIWIYLTAPILGTLCGAGIYTAVKLPDKDR 273
           LNVLIAG                       A+WIYLTAPILG LCGAGIYTAVKLPDKD 
Sbjct: 241 LNVLIAGQATGASMNPVRTLGPAIAANNFKAVWIYLTAPILGALCGAGIYTAVKLPDKDG 300

[Close]

BLAST of Lsi07G004050 vs. NCBI nr
Match: XP_008454166.1 (PREDICTED: aquaporin NIP6-1 [Cucumis melo] >TYK29615.1 aquaporin NIP6-1 [Cucumis melo var. makuwa])

HSP 1 Score: 450.7 bits (1158), Expect = 9.1e-123
Identity = 244/313 (77.96%), Postives = 251/313 (80.19%), Query Frame = 0


Query: 7   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFSVDDNWGTEEGSLSK 66
           MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKS I F+VDD+WG EEG LSK
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLSK 60

Query: 67  LVSSCSLPYPPVSLAR------------------------KTEGTETLIGLAASTGLAVM 126
           +VSSCSLPYPPVSLAR                        KTEGTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAVM 120

Query: 127 VVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPIMG 186
           +VILSTGHISGAHLNPA+TIAFAALKQFPWKHVP+YIGAQMVASLCSSFALKWIFDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180

Query: 187 GGATVPSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVGATVM 246
           GGATVPSCGYAQAFALEFIISFNLMFV+TAVATDTRA         VGELAGIAVGATVM
Sbjct: 181 GGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRA---------VGELAGIAVGATVM 240

Query: 247 LNVLIAG-----------------------AIWIYLTAPILGTLCGAGIYTAVKLPDKDR 273
           LNVLIAG                       AIWIYLTAPILGTLCGAGIYTAVKLPDKD 
Sbjct: 241 LNVLIAGQTTGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDG 300

[Close]

BLAST of Lsi07G004050 vs. NCBI nr
Match: XP_004152170.1 (aquaporin NIP6-1 [Cucumis sativus] >KGN52947.1 hypothetical protein Csa_015162 [Cucumis sativus])

HSP 1 Score: 450.3 bits (1157), Expect = 1.2e-122
Identity = 242/313 (77.32%), Postives = 251/313 (80.19%), Query Frame = 0


Query: 7   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFSVDDNWGTEEGSLSK 66
           MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISF+VDDNWG+EEG L+K
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60

Query: 67  LVSSCSLPYPPVSLAR------------------------KTEGTETLIGLAASTGLAVM 126
           +VSSCSLPY PVSLAR                        KT GTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120

Query: 127 VVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPIMG 186
           +VILSTGHISGAHLNPA+TIAFAALKQFPWKHVP+YIGAQMVASLCSSFALKW FDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWTFDPIMG 180

Query: 187 GGATVPSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVGATVM 246
           GGAT+PSCGYAQAFALEFIISFNLMFV+TAVATDTRA         VGELAGIAVGATVM
Sbjct: 181 GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRA---------VGELAGIAVGATVM 240

Query: 247 LNVLIAG-----------------------AIWIYLTAPILGTLCGAGIYTAVKLPDKDR 273
           LNVLIAG                       AIWIYLTAPILGTLCGAGIYTAVKLPDKD 
Sbjct: 241 LNVLIAGQTTGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDG 300

[Close]

BLAST of Lsi07G004050 vs. NCBI nr
Match: KAA0044488.1 (aquaporin NIP6-1 [Cucumis melo var. makuwa])

HSP 1 Score: 450.3 bits (1157), Expect = 1.2e-122
Identity = 243/313 (77.64%), Postives = 251/313 (80.19%), Query Frame = 0


Query: 7   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFSVDDNWGTEEGSLSK 66
           MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKS I F+VDD+WG EEG LSK
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLSK 60

Query: 67  LVSSCSLPYPPVSLAR------------------------KTEGTETLIGLAASTGLAVM 126
           +VSSCSLPYPPVSLAR                        KTEGTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAVM 120

Query: 127 VVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPIMG 186
           +VILSTGHISGAHLNPA+TIAFAALKQFPWKHVP+YIGAQMVASLCSSFALKWIFDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180

Query: 187 GGATVPSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVGATVM 246
           GGATVPSCGYAQAFALEFIISFNLMFV+TAVATDTRA         VGELAGIAVGATVM
Sbjct: 181 GGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRA---------VGELAGIAVGATVM 240

Query: 247 LNVLIAG-----------------------AIWIYLTAPILGTLCGAGIYTAVKLPDKDR 273
           LN+LIAG                       AIWIYLTAPILGTLCGAGIYTAVKLPDKD 
Sbjct: 241 LNILIAGQTTGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDG 300

[Close]

BLAST of Lsi07G004050 vs. NCBI nr
Match: XP_023524713.1 (aquaporin NIP6-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 389.4 bits (999), Expect = 2.5e-104
Identity = 218/302 (72.19%), Postives = 234/302 (77.48%), Query Frame = 0


Query: 7   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFSVDDNWGTEEGSLSK 66
           MDTEE PS PVTPGTPGAPLFG  K N HGSENGKRSLLKSCISF+V+D WGTEEGSLSK
Sbjct: 1   MDTEEVPS-PVTPGTPGAPLFGGHKGNHHGSENGKRSLLKSCISFNVND-WGTEEGSLSK 60

Query: 67  L----------VSSCSLPYPPVSLA---RKTEGTETLIGLAASTGLAVMVVILSTGHISG 126
           +          + +  L +   + A   +K +GTETLIGLAASTG+AVMVVILSTGHISG
Sbjct: 61  VSLARKVGAEFIGTLMLIFAGTATAIVNQKIQGTETLIGLAASTGMAVMVVILSTGHISG 120

Query: 127 AHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPIMGGGATVPSCGYA 186
           AHLNP ITIAFAALK FPWK VPVYIGAQ+VASLC+SFALKWIFDP MGGG TVPSCGY+
Sbjct: 121 AHLNPGITIAFAALKHFPWKQVPVYIGAQIVASLCASFALKWIFDPFMGGGVTVPSCGYS 180

Query: 187 QAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVGATVMLNVLIAG---- 246
           QAFALEFIISFNLMFVITAVATDTRA         VGELAGIAVGATVMLNVLIAG    
Sbjct: 181 QAFALEFIISFNLMFVITAVATDTRA---------VGELAGIAVGATVMLNVLIAGETTG 240

Query: 247 -------------------AIWIYLTAPILGTLCGAGIYTAVKLPDKDRDSRLPSTAGSF 273
                              AIWIYLTAPILGTLCGAGIYTA+KLPD+D D+RLPSTA SF
Sbjct: 241 ASMNPVRTLGPAIAANNFRAIWIYLTAPILGTLCGAGIYTAIKLPDQDGDARLPSTARSF 291

[Close]

BLAST of Lsi07G004050 vs. TAIR 10
Match: AT1G80760.1 (NOD26-like intrinsic protein 6;1 )

HSP 1 Score: 310.1 bits (793), Expect = 1.8e-84
Identity = 185/319 (57.99%), Postives = 207/319 (64.89%), Query Frame = 0


Query: 7   MDTEEAPSTP----VTPGTPGAPLFG--RVKENQHGSENGKRSLLKSCISFSVDDNWGTE 66
           MD EE PSTP     TPGTPGAPLFG    K N H      +SLLKSC  FSVD+ W  E
Sbjct: 1   MDHEEIPSTPSTPATTPGTPGAPLFGGFEGKRNGHNGRYTPKSLLKSCKCFSVDNEWALE 60

Query: 67  EGSLSKLVSSCSLPYPPVSLAR------------------------KTEGTETLIGLAAS 126
           +G L  +  +CSLP P VSL R                        KT+G ETLIG AAS
Sbjct: 61  DGRLPPV--TCSLPPPNVSLYRKLGAEFVGTLILIFAGTATAIVNQKTDGAETLIGCAAS 120

Query: 127 TGLAVMVVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWI 186
            GLAVM+VILSTGHISGAHLNPA+TIAFAALK FPWKHVPVYIGAQ++AS+ ++FALK +
Sbjct: 121 AGLAVMIVILSTGHISGAHLNPAVTIAFAALKHFPWKHVPVYIGAQVMASVSAAFALKAV 180

Query: 187 FDPIMGGGATVPSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIA 246
           F+P M GG TVP+ G +QAFALEFIISFNLMFV+TAVATDTRA         VGELAGIA
Sbjct: 181 FEPTMSGGVTVPTVGLSQAFALEFIISFNLMFVVTAVATDTRA---------VGELAGIA 240

Query: 247 VGATVMLNVLIAG-----------------------AIWIYLTAPILGTLCGAGIYTAVK 273
           VGATVMLN+LIAG                       AIW+YLTAPILG L GAG YT VK
Sbjct: 241 VGATVMLNILIAGPATSASMNPVRTLGPAIAANNYRAIWVYLTAPILGALIGAGTYTIVK 300

[Close]

BLAST of Lsi07G004050 vs. TAIR 10
Match: AT4G10380.1 (NOD26-like intrinsic protein 5;1 )

HSP 1 Score: 239.6 bits (610), Expect = 3.0e-63
Identity = 145/308 (47.08%), Postives = 176/308 (57.14%), Query Frame = 0


Query: 12  APSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFSVDDNWGTEEGSLSKLVSSC 71
           AP TP TPGTPG PL   ++ +    ++ K +    C+   +   WG  +   +      
Sbjct: 15  APPTPGTPGTPGGPLITGMRVDSMSFDHRKPTPRCKCLPV-MGSTWGQHDTCFT------ 74

Query: 72  SLPYPPVSLARKT------------------------EGTETLIGLAASTGLAVMVVILS 131
             P P VSL RK                         +G ETLIG AA  GLAVM++ILS
Sbjct: 75  DFPSPDVSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILS 134

Query: 132 TGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLCSSFALKWIFDPIMGGGATV 191
           TGHISGAHLNP++TIAFAAL+ FPW HVP YI AQ+ AS+C+SFALK +F P M GG T+
Sbjct: 135 TGHISGAHLNPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTI 194

Query: 192 PSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVELEMQVGELAGIAVGATVMLNVLI 251
           PS    QAFALEFII+F L+FV+TAVATDTRA         VGELAGIAVGATVMLN+L+
Sbjct: 195 PSVSLGQAFALEFIITFILLFVVTAVATDTRA---------VGELAGIAVGATVMLNILV 254

Query: 252 AG-----------------------AIWIYLTAPILGTLCGAGIYTAVKLPDKDRDSRLP 273
           AG                       ++W+YL AP LG + GA +YT VKL D   D   P
Sbjct: 255 AGPSTGGSMNPVRTLGPAVASGNYRSLWVYLVAPTLGAISGAAVYTGVKLNDSVTDP--P 304

[Close]

BLAST of Lsi07G004050 vs. TAIR 10
Match: AT5G37810.1 (NOD26-like intrinsic protein 4;1 )

HSP 1 Score: 146.7 bits (369), Expect = 2.6e-35
Identity = 87/199 (43.72%), Postives = 112/199 (56.28%), Query Frame = 0


Query: 86  GTETLIGLAASTGLAVMVVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVA 145
           GT T  G+  + GL VMV+I STGHISGAH NPA+T+ FA  ++FPW  VP+YIGAQ   
Sbjct: 71  GTITFPGICVTWGLIVMVMIYSTGHISGAHFNPAVTVTFAIFRRFPWHQVPLYIGAQFAG 130

Query: 146 SLCSSFALKWIF----DPIMGGGATVPSCGYAQAFALEFIISFNLMFVITAVATDTRAGD 205
           SL +S  L+ +F    +   G   T P+   A+A   E IISF LMFVI+ VATD RA  
Sbjct: 131 SLLASLTLRLMFKVTPEAFFG---TTPADSPARALVAEIIISFLLMFVISGVATDNRA-- 190

Query: 206 DVELEMQVGELAGIAVGATVMLNVLIAGA-----------------------IWIYLTAP 258
                  VGELAGIAVG T+M+NV +AG                        IW+Y+  P
Sbjct: 191 -------VGELAGIAVGMTIMVNVFVAGPISGASMNPARSLGPALVMGVYKHIWVYIVGP 250

[Close]

BLAST of Lsi07G004050 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 142.5 bits (358), Expect = 5.0e-34
Identity = 84/196 (42.86%), Postives = 109/196 (55.61%), Query Frame = 0


Query: 86  GTETLIGLAASTGLAVMVVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVA 145
           GT T  G+  + GL VMV+I STGHISGAH NPA+T+ FA  ++FPW  VP+YIGAQ+  
Sbjct: 71  GTITFPGICVTWGLIVMVMIYSTGHISGAHFNPAVTVTFAVFRRFPWYQVPLYIGAQLTG 130

Query: 146 SLCSSFALKWIFDPI-MGGGATVPSCGYAQAFALEFIISFNLMFVITAVATDTRAGDDVE 205
           SL +S  L+ +F+        T P+    QA   E IISF LMFVI+ VATD+RA     
Sbjct: 131 SLLASLTLRLMFNVTPKAFFGTTPTDSSGQALVAEIIISFLLMFVISGVATDSRA----- 190

Query: 206 LEMQVGELAGIAVGATVMLNVLIAG-----------------------AIWIYLTAPILG 258
                GELAGIAVG T++LNV +AG                        IW+Y+  P +G
Sbjct: 191 ----TGELAGIAVGMTIILNVFVAGPISGASMNPARSLGPAIVMGRYKGIWVYIVGPFVG 250

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BLAST of Lsi07G004050 vs. TAIR 10
Match: AT4G18910.1 (NOD26-like intrinsic protein 1;2 )

HSP 1 Score: 139.8 bits (351), Expect = 3.2e-33
Identity = 86/200 (43.00%), Postives = 118/200 (59.00%), Query Frame = 0


Query: 89  TLIGLAASTGLAVMVVILSTGHISGAHLNPAITIAFAALKQFPWKHVPVYIGAQMVASLC 148
           TL G+A   GL VMV++ S GHISGAH NPA+TIAFA+  +FP K VP Y+ +Q++ S  
Sbjct: 83  TLPGIAIVWGLTVMVLVYSLGHISGAHFNPAVTIAFASCGRFPLKQVPAYVISQVIGSTL 142

Query: 149 SSFALKWIF--DPIMGGG------ATVPSCGYAQAFALEFIISFNLMFVITAVATDTRAG 208
           ++  L+ +F  D  +  G       T+PS    Q+F +EFII+F LMFVI+ VATD RA 
Sbjct: 143 AAATLRLLFGLDQDVCSGKHDVFVGTLPSGSNLQSFVIEFIITFYLMFVISGVATDNRA- 202

Query: 209 DDVELEMQVGELAGIAVGATVMLNVLIAG-----------------------AIWIYLTA 258
                   +GELAG+AVG+TV+LNV+IAG                        +WIY+ +
Sbjct: 203 --------IGELAGLAVGSTVLLNVIIAGPVSGASMNPGRSLGPAMVYSCYRGLWIYIVS 262

The following BLAST results are available for this feature:

BLAST of Lsi07G004050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SAI4	2.5e-83	57.99	Aquaporin NIP6-1 OS=Arabidopsis thaliana OX=3702 GN=NIP6-1 PE=1 SV=1	[more]
Q9SV84	4.2e-62	47.08	Probable aquaporin NIP5-1 OS=Arabidopsis thaliana OX=3702 GN=NIP5-1 PE=2 SV=1	[more]
Q9ATN1	1.8e-52	44.98	Aquaporin NIP3-1 OS=Zea mays OX=4577 GN=NIP3-1 PE=2 SV=1	[more]
Q0IWF3	3.9e-52	44.16	Aquaporin NIP3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-1 PE=2 SV=2	[more]
Q0DK16	1.5e-35	42.58	Aquaporin NIP1-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-3 PE=3 SV=2	[more]

BLAST of Lsi07G004050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E2S7	4.4e-123	77.96	Aquaporin NIP6-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002...	[more]
A0A1S3BY36	4.4e-123	77.96	aquaporin NIP6-1 OS=Cucumis melo OX=3656 GN=LOC103494651 PE=3 SV=1	[more]
A0A5A7TNN6	5.8e-123	77.64	Aquaporin NIP6-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G0022...	[more]
A0A0A0KX08	5.8e-123	77.32	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007030 PE=3 SV=1	[more]
A0A6J1KEN1	7.9e-104	71.85	aquaporin NIP6-1 OS=Cucurbita maxima OX=3661 GN=LOC111492577 PE=3 SV=1	[more]

BLAST of Lsi07G004050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876197.1	6.3e-124	78.27	aquaporin NIP6-1 [Benincasa hispida]	[more]
XP_008454166.1	9.1e-123	77.96	PREDICTED: aquaporin NIP6-1 [Cucumis melo] >TYK29615.1 aquaporin NIP6-1 [Cucumis...	[more]
XP_004152170.1	1.2e-122	77.32	aquaporin NIP6-1 [Cucumis sativus] >KGN52947.1 hypothetical protein Csa_015162 [...	[more]
KAA0044488.1	1.2e-122	77.64	aquaporin NIP6-1 [Cucumis melo var. makuwa]	[more]
XP_023524713.1	2.5e-104	72.19	aquaporin NIP6-1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi07G004050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80760.1	1.8e-84	57.99	NOD26-like intrinsic protein 6;1	[more]
AT4G10380.1	3.0e-63	47.08	NOD26-like intrinsic protein 5;1	[more]
AT5G37810.1	2.6e-35	43.72	NOD26-like intrinsic protein 4;1	[more]
AT5G37820.1	5.0e-34	42.86	NOD26-like intrinsic protein 4;2	[more]
AT4G18910.1	3.2e-33	43.00	NOD26-like intrinsic protein 1;2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 134..153 score: 24.39 coord: 178..196 score: 27.17 coord: 97..121 score: 47.52 coord: 232..252 score: 31.1
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 86..230 e-value: 7.7E-25 score: 87.9
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10	Glycerol uptake facilitator protein.	coord: 67..261 e-value: 2.9E-37 score: 129.8
IPR023271	Aquaporin-like	SUPERFAMILY	81338	Aquaporin-like	coord: 84..259
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	PANTHER	PTHR45724:SF41	BNAA02G36290D PROTEIN	coord: 230..260 coord: 12..83
None	No IPR available	PANTHER	PTHR45724:SF41	BNAA02G36290D PROTEIN	coord: 82..229
IPR034294	Aquaporin transporter	PANTHER	PTHR45724	AQUAPORIN NIP2-1	coord: 82..229 coord: 12..83 coord: 230..260
IPR022357	Major intrinsic protein, conserved site	PROSITE	PS00221	MIP	coord: 115..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi07G004050.1	Lsi07G004050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0015267	channel activity