Lsi07G003990 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi07G003990
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Auxin-responsive GH3 family protein
Location	chr07: 4299410 .. 4301813 (+)
RNA-Seq Expression	Lsi07G003990
Synteny	Lsi07G003990

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTAATCCTTTCCCCTTCTGCCTCCCTGCCATTACAGATACCTGGAAAACAGAATCCCTTCACAAAGAAAAAGAAAAAGAAAAAAAAAAAAATTGCATAGTGAAATTGTCAGTTTATAGTTATAGTTATGAACTGATTGAGAGAGAAAAAGAAAAAAAAAAAAAAGCTACCTTTCTTTTGCTGTTTCATTCATTGCAATGTTTAAAATTTAAAAGTCAAGCACTTTCTCTTCAAATTCAAACCCTTTTTTGTTTCTTCTTCTTCTTCTTTAATTCTTCCTGTTCTGATTTGAAAAGATGTTGCCAAAATTTGATCCCAACAACAATGAATCTGGGTTGAAGCTGCTTGAAGATCTTACACAAAATGCAAAGCAAATTCAAGAACAAGTGATTGAGAAGATTCTTACACAAAACTTCAACACAGAATATCTCAAGACTTTTCTCAATGGCCAATCTCCTGATCTCCAAAGTTTCAAGAACTCTGTTCCTGTGGTCAACTATGAAGACATTAAGCCTTATATTGAGAGAATCGCCAATGGTGAACCTTCTCATATAATTTCATCTCAACCCATCACTGAGCTACTCACAAGGTAAATATTAAATTCATCCATTTTTCAACACTTTCAAAAGAAAATTTTCATACTCTCAACCAAATTTGCAGCTCTGGAACTTCTGGAGGACAGCCAAAAATGATGCCCTCAACTGCTGAGGATTTGGACAGAAAGACATTCTTTTACAATCTCCTTGTACCTGTTTTGAACAAGTATGAATAAAACAGTAAACTTTATCATTATTGTTCTAATTTTTTTCTTTTTTCTTTCTTTCTTTAAACATTATAGAGGAAAATTGTTGAAATTTCAGGTATGTGGATGGTTTGGAAGAAGGAAAAGGGATGTATCTTTTGTTTATAAAGCCAGAAATGAGTACCCCATGTGGGTTAATGGCAAGGCCAGTTTTGACAAGTTATTACAAAAGCAAAAACTTCAGAAATAGGCCTTTTAATAGATACAATGTGTACACAAGCCCAGATGAGACAATTCTTTGTTCTGACAGTAAACAGAGTATGTACTGTCAATTGCTTTGTGGGTTATTACAAAGAGATGAAGTTTTAAGAGTTGGTGCAGTTTTTGCCTCTGCTTTTCTTCGAGCTATCAAATTCTTAGAAGATTATTGGAAAGAATTGAGTGATAATATAAGAAATGGGGAAGTTAGTGAATGGATTACTGACTCAAATTGCAGAGCTTCTGTTTCTTTAATCCTTACAAAACCAAATGCTGCTTTGGCTGATTTGATTGATGGTATGTGTGATGAGAAATCATGGGAAGGAATAATTAAGAAGATTTGGCCTAAAACTAAATACATTGAAGTTATTGTAACTGGGTCTATGGCTCAGTATATTCCTACTCTTGAATTTTACAGTGGTGGGCTTCCTTTAGTTTCTACTATGTATGCTTCTTCTGAGTGTTATTTTGGGATTAATTTCAATCCTTTGAGTAAACCTTGTGATGTTTCTTATACACTTCTTCCAAATATGGCCTTCTTTGAATTCTTACCTGTGGAGAAAAATGATGGAGAATTAAGCCATTGCAATGGAAGCTCTGTTCAATCAATTGATGAATTCAAAACAGTTGATCTTGTCGATGTCAAACTTGGTCAATATTACGAATTGGTGGTCACTACTTTCACAGGTGAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAACCCTTTTTTTGTTTTTTGTTTTTGGATCTGACCCACATTATTTTTGCTCTGTTTTTTTTTTTTTTTTTTCTTTTTGGTGGTGGGTTCAGGGTTGTACAGATACAGAGTTGGTGATATTCTAATGGTAACTGGCTTCCACAACAAAGCACCTCAATTCAAGTTTGTTCATAGAAGGAATGTTGTTTTAAGCATAGATACAGATAAGACGAATGAAGATGATTTGTTAAATGCAATTACAAAAGCGAAATTACTTGTTGAACCACTTGGTGTTTTGTTAACAGAGTATACAAGTTATGCAGATACAGGGTCAATTCCAGGGCATTATGTTCTGTTTTGGGAGCTGAAGAAAAGAGGAGGGAATGAGAATTTAGAAGAACTTGAAGTGGGTTTAATGGAAGAATGTTGTTCAAGTATAGAACAATCATTGGATTCAGTTTATAGAAGATGCAGAAGCAAAGATAAATCAATTGGGCCATTGGAGATTAGAGTTGTGAAACATGGAGCTTTTGATGCACTTATGGATTTTTGTGTTTCACAAGGATCTTCTGTGAATCAATACAAAACACCAAGATGCATTAAATCAGAAGAAGCCATCAAAATTCTGGATTCTAGAGTTGTGGGAAGGTATTTCAGCACAAAAACACCATTTTGGGAGCCTTTCAGAGCTCATTACTCCCATTGA

mRNA sequence

ATTTAATCCTTTCCCCTTCTGCCTCCCTGCCATTACAGATACCTGGAAAACAGAATCCCTTCACAAAGAAAAAGAAAAAGAAAAAAAAAAAAATTGCATAGTGAAATTGTCAGTTTATAGTTATAGTTATGAACTGATTGAGAGAGAAAAAGAAAAAAAAAAAAAAGCTACCTTTCTTTTGCTGTTTCATTCATTGCAATGTTTAAAATTTAAAAGTCAAGCACTTTCTCTTCAAATTCAAACCCTTTTTTGTTTCTTCTTCTTCTTCTTTAATTCTTCCTGTTCTGATTTGAAAAGATGTTGCCAAAATTTGATCCCAACAACAATGAATCTGGGTTGAAGCTGCTTGAAGATCTTACACAAAATGCAAAGCAAATTCAAGAACAAGTGATTGAGAAGATTCTTACACAAAACTTCAACACAGAATATCTCAAGACTTTTCTCAATGGCCAATCTCCTGATCTCCAAAGTTTCAAGAACTCTGTTCCTGTGGTCAACTATGAAGACATTAAGCCTTATATTGAGAGAATCGCCAATGGTGAACCTTCTCATATAATTTCATCTCAACCCATCACTGAGCTACTCACAAGCTCTGGAACTTCTGGAGGACAGCCAAAAATGATGCCCTCAACTGCTGAGGATTTGGACAGAAAGACATTCTTTTACAATCTCCTTGTACCTGTTTTGAACAAGTATGTGGATGGTTTGGAAGAAGGAAAAGGGATGTATCTTTTGTTTATAAAGCCAGAAATGAGTACCCCATGTGGGTTAATGGCAAGGCCAGTTTTGACAAGTTATTACAAAAGCAAAAACTTCAGAAATAGGCCTTTTAATAGATACAATGTGTACACAAGCCCAGATGAGACAATTCTTTGTTCTGACAGTAAACAGAGTATGTACTGTCAATTGCTTTGTGGGTTATTACAAAGAGATGAAGTTTTAAGAGTTGGTGCAGTTTTTGCCTCTGCTTTTCTTCGAGCTATCAAATTCTTAGAAGATTATTGGAAAGAATTGAGTGATAATATAAGAAATGGGGAAGTTAGTGAATGGATTACTGACTCAAATTGCAGAGCTTCTGTTTCTTTAATCCTTACAAAACCAAATGCTGCTTTGGCTGATTTGATTGATGGTATGTGTGATGAGAAATCATGGGAAGGAATAATTAAGAAGATTTGGCCTAAAACTAAATACATTGAAGTTATTGTAACTGGGTCTATGGCTCAGTATATTCCTACTCTTGAATTTTACAGTGGTGGGCTTCCTTTAGTTTCTACTATGTATGCTTCTTCTGAGTGTTATTTTGGGATTAATTTCAATCCTTTGAGTAAACCTTGTGATGTTTCTTATACACTTCTTCCAAATATGGCCTTCTTTGAATTCTTACCTGTGGAGAAAAATGATGGAGAATTAAGCCATTGCAATGGAAGCTCTGTTCAATCAATTGATGAATTCAAAACAGTTGATCTTGTCGATGTCAAACTTGGTCAATATTACGAATTGGTGGTCACTACTTTCACAGGGTTGTACAGATACAGAGTTGGTGATATTCTAATGGTAACTGGCTTCCACAACAAAGCACCTCAATTCAAGTTTGTTCATAGAAGGAATGTTGTTTTAAGCATAGATACAGATAAGACGAATGAAGATGATTTGTTAAATGCAATTACAAAAGCGAAATTACTTGTTGAACCACTTGGTGTTTTGTTAACAGAGTATACAAGTTATGCAGATACAGGGTCAATTCCAGGGCATTATGTTCTGTTTTGGGAGCTGAAGAAAAGAGGAGGGAATGAGAATTTAGAAGAACTTGAAGTGGGTTTAATGGAAGAATGTTGTTCAAGTATAGAACAATCATTGGATTCAGTTTATAGAAGATGCAGAAGCAAAGATAAATCAATTGGGCCATTGGAGATTAGAGTTGTGAAACATGGAGCTTTTGATGCACTTATGGATTTTTGTGTTTCACAAGGATCTTCTGTGAATCAATACAAAACACCAAGATGCATTAAATCAGAAGAAGCCATCAAAATTCTGGATTCTAGAGTTGTGGGAAGGTATTTCAGCACAAAAACACCATTTTGGGAGCCTTTCAGAGCTCATTACTCCCATTGA

Coding sequence (CDS)

ATGTTGCCAAAATTTGATCCCAACAACAATGAATCTGGGTTGAAGCTGCTTGAAGATCTTACACAAAATGCAAAGCAAATTCAAGAACAAGTGATTGAGAAGATTCTTACACAAAACTTCAACACAGAATATCTCAAGACTTTTCTCAATGGCCAATCTCCTGATCTCCAAAGTTTCAAGAACTCTGTTCCTGTGGTCAACTATGAAGACATTAAGCCTTATATTGAGAGAATCGCCAATGGTGAACCTTCTCATATAATTTCATCTCAACCCATCACTGAGCTACTCACAAGCTCTGGAACTTCTGGAGGACAGCCAAAAATGATGCCCTCAACTGCTGAGGATTTGGACAGAAAGACATTCTTTTACAATCTCCTTGTACCTGTTTTGAACAAGTATGTGGATGGTTTGGAAGAAGGAAAAGGGATGTATCTTTTGTTTATAAAGCCAGAAATGAGTACCCCATGTGGGTTAATGGCAAGGCCAGTTTTGACAAGTTATTACAAAAGCAAAAACTTCAGAAATAGGCCTTTTAATAGATACAATGTGTACACAAGCCCAGATGAGACAATTCTTTGTTCTGACAGTAAACAGAGTATGTACTGTCAATTGCTTTGTGGGTTATTACAAAGAGATGAAGTTTTAAGAGTTGGTGCAGTTTTTGCCTCTGCTTTTCTTCGAGCTATCAAATTCTTAGAAGATTATTGGAAAGAATTGAGTGATAATATAAGAAATGGGGAAGTTAGTGAATGGATTACTGACTCAAATTGCAGAGCTTCTGTTTCTTTAATCCTTACAAAACCAAATGCTGCTTTGGCTGATTTGATTGATGGTATGTGTGATGAGAAATCATGGGAAGGAATAATTAAGAAGATTTGGCCTAAAACTAAATACATTGAAGTTATTGTAACTGGGTCTATGGCTCAGTATATTCCTACTCTTGAATTTTACAGTGGTGGGCTTCCTTTAGTTTCTACTATGTATGCTTCTTCTGAGTGTTATTTTGGGATTAATTTCAATCCTTTGAGTAAACCTTGTGATGTTTCTTATACACTTCTTCCAAATATGGCCTTCTTTGAATTCTTACCTGTGGAGAAAAATGATGGAGAATTAAGCCATTGCAATGGAAGCTCTGTTCAATCAATTGATGAATTCAAAACAGTTGATCTTGTCGATGTCAAACTTGGTCAATATTACGAATTGGTGGTCACTACTTTCACAGGGTTGTACAGATACAGAGTTGGTGATATTCTAATGGTAACTGGCTTCCACAACAAAGCACCTCAATTCAAGTTTGTTCATAGAAGGAATGTTGTTTTAAGCATAGATACAGATAAGACGAATGAAGATGATTTGTTAAATGCAATTACAAAAGCGAAATTACTTGTTGAACCACTTGGTGTTTTGTTAACAGAGTATACAAGTTATGCAGATACAGGGTCAATTCCAGGGCATTATGTTCTGTTTTGGGAGCTGAAGAAAAGAGGAGGGAATGAGAATTTAGAAGAACTTGAAGTGGGTTTAATGGAAGAATGTTGTTCAAGTATAGAACAATCATTGGATTCAGTTTATAGAAGATGCAGAAGCAAAGATAAATCAATTGGGCCATTGGAGATTAGAGTTGTGAAACATGGAGCTTTTGATGCACTTATGGATTTTTGTGTTTCACAAGGATCTTCTGTGAATCAATACAAAACACCAAGATGCATTAAATCAGAAGAAGCCATCAAAATTCTGGATTCTAGAGTTGTGGGAAGGTATTTCAGCACAAAAACACCATTTTGGGAGCCTTTCAGAGCTCATTACTCCCATTGA

Protein sequence

MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFKNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKNDGELSHCNGSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTGSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEPFRAHYSH

Homology

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BLAST of Lsi07G003990 vs. ExPASy Swiss-Prot
Match: Q9FZ87 (Indole-3-acetic acid-amido synthetase GH3.17 OS=Arabidopsis thaliana OX=3702 GN=GH3.17 PE=1 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 3.5e-258
Identity = 435/605 (71.90%), Postives = 515/605 (85.12%), Query Frame = 0


Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQS-PDLQSF 60
           M+P +DPN+ E+GLKLLEDLT NA+ IQ+QV+ +IL+QN  T+YL+ FL+G++  + QSF
Sbjct: 1   MIPSYDPNDTEAGLKLLEDLTTNAEAIQQQVLHQILSQNSGTQYLRAFLDGEADKNQQSF 60

Query: 61  KNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KN VPVVNY+D+KP+I+RIA+GE S I+S+QPITELLTSSGTS G+PK+MPSTAE+L+RK
Sbjct: 61  KNKVPVVNYDDVKPFIQRIADGESSDIVSAQPITELLTSSGTSAGKPKLMPSTAEELERK 120

Query: 121 TFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFN 180
           TFFY++LVP++NKYVDGL+EGKGMYLLFIKPE+ TP GLMARPVLTSYYKS++FRNRPFN
Sbjct: 121 TFFYSMLVPIMNKYVDGLDEGKGMYLLFIKPEIKTPSGLMARPVLTSYYKSQHFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKEL 240
           +YNVYTSPD+TILC DSKQSMYCQLLCGL+QR  VLRVGAVFASAFLRA+KFLED++KEL
Sbjct: 181 KYNVYTSPDQTILCQDSKQSMYCQLLCGLVQRSHVLRVGAVFASAFLRAVKFLEDHYKEL 240

Query: 241 SDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYI 300
             +IR G V+ WITDS+CR SV  IL  PN  LAD I+  C EKSWEGI+++IWPK KY+
Sbjct: 241 CADIRTGTVTSWITDSSCRDSVLSILNGPNQELADEIESECAEKSWEGILRRIWPKAKYV 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGIN NPL  P DVSYTLLPNMA+F
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINLNPLCDPADVSYTLLPNMAYF 360

Query: 361 EFLPV-EKNDGELSHCNGSSVQSID----EFKTVDLVDVKLGQYYELVVTTFTGLYRYRV 420
           EFLPV +K+  E+     S+    D    E   V+LV+V++GQYYE+V+TTFTGLYRYRV
Sbjct: 361 EFLPVDDKSHEEIHFATHSNTDDDDDALKEDLIVNLVNVEVGQYYEIVITTFTGLYRYRV 420

Query: 421 GDILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKL--LVEPLGVLLTE 480
           GDIL VTGFHNKAPQF+FV RRNVVLSIDTDKT+E+DLLNA+T+AKL  L  P  +LLTE
Sbjct: 421 GDILKVTGFHNKAPQFRFVQRRNVVLSIDTDKTSEEDLLNAVTQAKLNHLQHPSSLLLTE 480

Query: 481 YTSYADTGSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDK 540
           YTSYADT SIPGHYVLFWELK R  N+   +L+   ME+CCS +E  LD VYRRCR++DK
Sbjct: 481 YTSYADTSSIPGHYVLFWELKPRHSNDP-PKLDDKTMEDCCSEVEDCLDYVYRRCRNRDK 540

Query: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKT 598
           SIGPLEIRVV  G FD+LMDFCVSQGSS+NQYKTPRC+KS  A++ILDSRV+GR+FS + 
Sbjct: 541 SIGPLEIRVVSLGTFDSLMDFCVSQGSSLNQYKTPRCVKSGGALEILDSRVIGRFFSKRV 600

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BLAST of Lsi07G003990 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 7.1e-203
Identity = 350/590 (59.32%), Postives = 452/590 (76.61%), Query Frame = 0


Query: 10  NESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTF-LNGQSPDLQSFKNSVPVVNY 69
           N+  L+L+E+LT NA Q+Q QV+E+ILT+N + EYL+   LNG++ D ++FKN +PV+ Y
Sbjct: 21  NKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRT-DRETFKNIMPVITY 80

Query: 70  EDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVP 129
           EDI+P I RIANG+ S I+SS+PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+LL+P
Sbjct: 81  EDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMP 140

Query: 130 VLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPD 189
           V++++V GLE GKGMY LFIK E  TP GL ARPVLTSYYKS +F+ RP++ Y  YTSP+
Sbjct: 141 VMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPN 200

Query: 190 ETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEV 249
           ETILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE +W EL  +IR G +
Sbjct: 201 ETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTL 260

Query: 250 SEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMA 309
           S  ITD + R +V+ IL KP+  LAD ++  C + SW+GII ++WP TKY++VIVTG+M+
Sbjct: 261 SSLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMS 320

Query: 310 QYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKND 369
           QYIPTL++YS GLPLV TMYASSECYFG+N  PL KP +VSYTL+P+MA+FEFLPV +N+
Sbjct: 321 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNN 380

Query: 370 GELSHCN-GSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKA 429
           G  +  N   ++   ++ + VDLVDVKLGQ YELVVTT+ GL RYRVGD+L VTGF NKA
Sbjct: 381 GVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNKA 440

Query: 430 PQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTGSIPGHYV 489
           PQF F+ R+NVVLSID+DKT+E +L NA+  A   + P    L+EYTSYADT SIPGHYV
Sbjct: 441 PQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYV 500

Query: 490 LFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAF 549
           LFWEL   G       +   + E+CC ++E+S ++VYR+ R  DKSIGPLEI++V+ G F
Sbjct: 501 LFWELCLDGNT----PIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTF 560

Query: 550 DALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEP 598
           D LMD+ +S G+S+NQYKTPRC+K    I++L+SRVV  YFS K P W P
Sbjct: 561 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604

[Close]

BLAST of Lsi07G003990 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 4.6e-202
Identity = 347/590 (58.81%), Postives = 450/590 (76.27%), Query Frame = 0


Query: 10  NESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTF-LNGQSPDLQSFKNSVPVVNY 69
           N++ L+ +ED+T NA  +Q +V+E+IL++N + EYLK   L G++ D ++FK+ +PVV Y
Sbjct: 21  NKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRT-DRETFKHIMPVVTY 80

Query: 70  EDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVP 129
           EDI+P I RIANG+ S ++ S PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+LL+P
Sbjct: 81  EDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMP 140

Query: 130 VLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPD 189
           V++++V GL++GKGMY LFIK E  TP GL ARPVLTSYYKS +F+NRP++ Y  YTSP+
Sbjct: 141 VMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPN 200

Query: 190 ETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEV 249
           +TILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE +W EL+ +IR G +
Sbjct: 201 QTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTL 260

Query: 250 SEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMA 309
           S  ITDS+ R +V  IL KP+  LAD ++  C + SW+GII ++WP TKY++VIVTG+M+
Sbjct: 261 SSEITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMS 320

Query: 310 QYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKND 369
           QYIPTL++YS GLPLV TMYASSECYFG+N  PL KP +VSYTL+PNMA+FEFLPV +N 
Sbjct: 321 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNS 380

Query: 370 GELSHCN-GSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKA 429
           G  S  +   ++   ++ + VDLVDVKLGQ YELVVTT+ GLYRYRVGD+L V GF N A
Sbjct: 381 GVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNA 440

Query: 430 PQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTGSIPGHYV 489
           PQF F+ R+NVVLSID+DKT+E +L NA+  A   + P    L+EYTSYADT SIPGHYV
Sbjct: 441 PQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYV 500

Query: 490 LFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAF 549
           LFWEL   G       +   + E+CC +IE+SL+SVYR+ R  DKSIGPLEI++V+ G F
Sbjct: 501 LFWELCLNGNT----PIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTF 560

Query: 550 DALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEP 598
           D LMD+ +S G+S+NQYKTPRC+K    I++L+SRVV  YFS K P W P
Sbjct: 561 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

[Close]

BLAST of Lsi07G003990 vs. ExPASy Swiss-Prot
Match: P0C0M3 (Probable indole-3-acetic acid-amido synthetase GH3.11 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.11 PE=2 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 2.6e-197
Identity = 337/581 (58.00%), Postives = 437/581 (75.22%), Query Frame = 0


Query: 14  LKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFKNSVPVVNYEDIKP 73
           L+ LE LT NAK+ QE ++ KIL +N  TEYL  F+NG S ++ +FK  VPVV Y+ + P
Sbjct: 14  LEELEMLTVNAKEAQELILTKILERNQATEYLSKFMNG-STNISAFKRHVPVVTYDKVHP 73

Query: 74  YIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVPVLNKY 133
           YI RIA GE S I+  + I ELL SSGTS G+P++MPS  +DLDR+T+ Y+L++P++NKY
Sbjct: 74  YILRIATGEESSILCGEYILELLRSSGTSRGEPRLMPSILKDLDRRTYLYSLIMPIMNKY 133

Query: 134 VDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDETILC 193
           + GL EGK MYLLF+K E  T  G+  R VLTSYYKS +F +R  + YN YTSPDE ILC
Sbjct: 134 ISGLGEGKAMYLLFVKAETLTDSGIPVRSVLTSYYKSPHFLHRKHDLYNNYTSPDEVILC 193

Query: 194 SDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEVSEWIT 253
            DS+QSMYCQLLCGL++R  VLR+GAVFASAFLR+I FLE +W++L ++IR G+++  IT
Sbjct: 194 PDSQQSMYCQLLCGLVERQHVLRIGAVFASAFLRSISFLEQHWRDLVNDIRIGQLNSSIT 253

Query: 254 DSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMAQYIPT 313
              CR ++   L  PN  LAD ++ +C   SW+GI+ ++WP  KYIE ++TG+MAQYIP 
Sbjct: 254 SPACRLAMLNFLALPNPELADQVEAICSCGSWKGILGRLWPNVKYIEAVLTGTMAQYIPM 313

Query: 314 LEFYSGG-LPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKNDGELS 373
           LEFY GG +P V TMYASSE YFG+N +PL  P DVSYT+LPNMA+FEF+P+E       
Sbjct: 314 LEFYGGGAIPFVCTMYASSESYFGVNLSPLCSPADVSYTILPNMAYFEFIPLEDGLRLTD 373

Query: 374 HCNGSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKAPQFKF 433
           H      + I+  K V LVDVK+G YYELVVTTF+GLYRYRVGD+L VTGF+N+APQFKF
Sbjct: 374 H-----EEVIENDKLVSLVDVKVGCYYELVVTTFSGLYRYRVGDVLQVTGFYNRAPQFKF 433

Query: 434 VHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTGSIPGHYVLFWEL 493
           + RRNV+LSID+DKTNE+DL N++T AK ++E    LL EYTSY D  ++PGHYVLFWE+
Sbjct: 434 ICRRNVILSIDSDKTNEEDLHNSVTTAKKILENQNYLLLEYTSYTDISTVPGHYVLFWEI 493

Query: 494 KKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAFDALMD 553
           K    +E    L+  L+E CC+++E+SLD VYRRCR+ D+SIGPLEIR+V+ GAFDALMD
Sbjct: 494 KST-HDERPAPLDAQLLESCCAAVEESLDYVYRRCRAHDRSIGPLEIRLVEAGAFDALMD 553

Query: 554 FCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTP 594
             VS GSS+NQYKTPRCI+S  A+K+L+S+V+  +FS + P
Sbjct: 554 LLVSHGSSINQYKTPRCIESSLALKLLNSKVIACFFSPQDP 587

[Close]

BLAST of Lsi07G003990 vs. ExPASy Swiss-Prot
Match: Q8GZ29 (Indole-3-acetic acid-amido synthetase GH3.15 OS=Arabidopsis thaliana OX=3702 GN=GH3.15 PE=1 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 3.8e-196
Identity = 341/601 (56.74%), Postives = 434/601 (72.21%), Query Frame = 0


Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFK 60
           MLPKFDP N ++ L LLEDLT N KQIQ+ V+E IL++N  TEYL+ FLNGQ  D Q+FK
Sbjct: 1   MLPKFDPTNQKACLSLLEDLTTNVKQIQDSVLEAILSRNAQTEYLRGFLNGQ-VDKQNFK 60

Query: 61  NSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKT 120
            +VPVV YEDI+ YI+RIANGEPS +I  +PI+ LLTSSGTSGG PK++P T EDL+++ 
Sbjct: 61  KNVPVVTYEDIRSYIDRIANGEPSDLICDRPISVLLTSSGTSGGVPKLIPLTTEDLEQRI 120

Query: 121 FFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNR 180
            F +L  P+L K++DGL EGK +   F+  E  T  GLM R ++TS+ KS    N  F  
Sbjct: 121 SFSSLYAPLLYKHIDGLSEGKSLIFYFVTRESKTANGLMVRTMVTSFLKSIKQTN-SFLW 180

Query: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELS 240
            ++  SP     C+D+ QSMYCQLLCGLL+RD V R+GA FAS+FL+ IKFLED+W EL 
Sbjct: 181 DSLQVSPHAITTCADTTQSMYCQLLCGLLERDNVARLGAPFASSFLKVIKFLEDHWPELC 240

Query: 241 DNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIE 300
            NIR G +S+WITD+ C + +   LT PN  LA LI+  C + SWE I+K++WPK K IE
Sbjct: 241 SNIRTGRLSDWITDATCTSGIGKFLTAPNPELASLIEQECSKTSWEAILKRLWPKAKCIE 300

Query: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFE 360
            I+TG+MAQYIP LEFYSGGLPL S+ Y SSEC+ G+NFNPL KP DVSYT++P M +FE
Sbjct: 301 SIITGTMAQYIPLLEFYSGGLPLTSSFYGSSECFMGVNFNPLCKPSDVSYTIIPCMGYFE 360

Query: 361 FLPVEKNDGELSHCNGSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMV 420
           FL VEK+  E  H         ++   VDLVDVK+G  YE VVTTF+GLYRYRVGD+L  
Sbjct: 361 FLEVEKDHQEAGH------DPTEKPVVVDLVDVKIGHDYEPVVTTFSGLYRYRVGDVLRA 420

Query: 421 TGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTG 480
           TGF+N AP F FV R+ VVLSID DKT EDDLL A+T AKLL+EP  ++L ++TS  D+ 
Sbjct: 421 TGFYNNAPHFCFVGRQKVVLSIDMDKTYEDDLLKAVTNAKLLLEPHDLMLMDFTSRVDSS 480

Query: 481 SIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIR 540
           S PGHYV++WEL  +  +   E     +MEECC ++E+SLD+VYR+ R  DK+IGPLEI+
Sbjct: 481 SFPGHYVIYWELGSKVKDAKFEP-NRDVMEECCFTVEESLDAVYRKGRKNDKNIGPLEIK 540

Query: 541 VVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEPFRA 600
           VVK GAFD LM+F +S+GSSV+QYKTPR + +EEA+KIL++ V+  + S K P WE    
Sbjct: 541 VVKPGAFDELMNFFLSRGSSVSQYKTPRSVTNEEALKILEANVISEFLSRKIPSWELHEL 592

Query: 601 H 602
           H
Sbjct: 601 H 592

[Close]

BLAST of Lsi07G003990 vs. ExPASy TrEMBL
Match: A0A6J1ISD7 (indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita maxima OX=3661 GN=LOC111480114 PE=3 SV=1)

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 549/604 (90.89%), Postives = 575/604 (95.20%), Query Frame = 0


Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNG-QSPDLQSF 60
           ML  FDPN+NE GLKLLEDLTQNA+QIQEQVIEKILTQN NTEYLK+F+NG  SPD+  F
Sbjct: 1   MLSSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 61  KNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KN+VPVVNYEDIKPYIERIANGEPS+IIS+QPITELLTSSGTSGGQPKMMPSTAEDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120

Query: 121 TFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFN 180
           TFFYNLLVPV+NKYVDGLE+GKGMYLLFIKPE+STP GLMARPVLTSYYKSKNFRNRPFN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKEL 240
           RYNVYTSPDETILC+DS+QSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLED+WKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 241 SDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYI 300
           SDNIR+G VS+WITD NCRASVSL+LTKPNAALADLID MC EK WEGIIKKIWPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDSMCGEKLWEGIIKKIWPKTKYI 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKP DVSYTLLPNMAFF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 361 EFLPVEKNDGELSHCNGSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILM 420
           EFLPVEKN+GELSHCNG SV   DEF+TVDLVDVKLG YYELVVTTFTGLYRYRVGDILM
Sbjct: 361 EFLPVEKNNGELSHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 420

Query: 421 VTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADT 480
           VTGFHN APQF+FVHRRNVVLSIDTDKTNEDDLLNAITKAKLL+EPLGVLLTEYTSYADT
Sbjct: 421 VTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTSYADT 480

Query: 481 GSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEI 540
           GSIPGHYVLFWELK RGG E+L ELE  LMEECCSS+EQSLDSVYRRCRSKDKSIGPLEI
Sbjct: 481 GSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEQSLDSVYRRCRSKDKSIGPLEI 540

Query: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEPFR 600
           RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSR VGR+FS KTPFWEPFR
Sbjct: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRAVGRFFSRKTPFWEPFR 600

Query: 601 AHYS 604
           A YS
Sbjct: 601 AQYS 604

[Close]

BLAST of Lsi07G003990 vs. ExPASy TrEMBL
Match: A0A5A7TMG2 (Indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002300 PE=3 SV=1)

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 557/612 (91.01%), Postives = 578/612 (94.44%), Query Frame = 0


Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLN----GQSPDL 60
           MLP FDPNNNESGLKLLEDLT NAKQIQEQVI KILTQN NTEYLK+FL+      S DL
Sbjct: 1   MLPNFDPNNNESGLKLLEDLTTNAKQIQEQVIRKILTQNSNTEYLKSFLHNHHYSHSLDL 60

Query: 61  QSFKNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL 120
           Q+FK+SVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL
Sbjct: 61  QTFKHSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL 120

Query: 121 DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNR 180
           DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTP GLMARPVLTSYYKSKNFRNR
Sbjct: 121 DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRNR 180

Query: 181 PFNRYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYW 240
           PFN+YNVYTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLEDYW
Sbjct: 181 PFNKYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDYW 240

Query: 241 KELSDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKT 300
           KELSDNIR GE+S+WI+D NCRASVSLILTK N  LADLIDG+C EKSWEGIIKK+WPKT
Sbjct: 241 KELSDNIRKGELSQWISDPNCRASVSLILTKSNPVLADLIDGLCGEKSWEGIIKKLWPKT 300

Query: 301 KYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNM 360
           KYIEVIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINFNPLSKP DVSYTLLPNM
Sbjct: 301 KYIEVIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPSDVSYTLLPNM 360

Query: 361 AFFEFLPVEKNDGELSHCNGSSVQSI-DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVG 420
           AFFEFLPVEKNDGEL+HCNG++  S+ DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVG
Sbjct: 361 AFFEFLPVEKNDGELTHCNGTTNTSVQDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVG 420

Query: 421 DILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTS 480
           DIL VTGFHNKAPQFKF+HRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTS
Sbjct: 421 DILKVTGFHNKAPQFKFIHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTS 480

Query: 481 YADTGSIPGHYVLFWELKKRGGN--ENLEEL-EVGLMEECCSSIEQSLDSVYRRCRSKDK 540
           YADT SIPGHYVLFWE+K+R G+  EN EEL EV LMEECCSSIE+SLDSVYRRCRSKDK
Sbjct: 481 YADTESIPGHYVLFWEMKRRSGSNYENFEELVEVDLMEECCSSIEESLDSVYRRCRSKDK 540

Query: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKT 600
           SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVV RYFS KT
Sbjct: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVARYFSKKT 600

Query: 601 PFWEPFRAHYSH 605
           PFWEPFR  YSH
Sbjct: 601 PFWEPFRGQYSH 612

[Close]

BLAST of Lsi07G003990 vs. ExPASy TrEMBL
Match: A0A1S3BYQ7 (indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo OX=3656 GN=LOC103494645 PE=3 SV=1)

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 557/612 (91.01%), Postives = 578/612 (94.44%), Query Frame = 0


Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLN----GQSPDL 60
           MLP FDPNNNESGLKLLEDLT NAKQIQEQVI KILTQN NTEYLK+FL+      S DL
Sbjct: 1   MLPNFDPNNNESGLKLLEDLTTNAKQIQEQVIRKILTQNSNTEYLKSFLHNHHYSHSLDL 60

Query: 61  QSFKNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL 120
           Q+FK+SVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL
Sbjct: 61  QTFKHSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL 120

Query: 121 DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNR 180
           DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTP GLMARPVLTSYYKSKNFRNR
Sbjct: 121 DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRNR 180

Query: 181 PFNRYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYW 240
           PFN+YNVYTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLEDYW
Sbjct: 181 PFNKYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDYW 240

Query: 241 KELSDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKT 300
           KELSDNIR GE+S+WI+D NCRASVSLILTK N  LADLIDG+C EKSWEGIIKK+WPKT
Sbjct: 241 KELSDNIRKGELSQWISDPNCRASVSLILTKSNPVLADLIDGLCGEKSWEGIIKKLWPKT 300

Query: 301 KYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNM 360
           KYIEVIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINFNPLSKP DVSYTLLPNM
Sbjct: 301 KYIEVIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPSDVSYTLLPNM 360

Query: 361 AFFEFLPVEKNDGELSHCNGSSVQSI-DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVG 420
           AFFEFLPVEKNDGEL+HCNG++  S+ DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVG
Sbjct: 361 AFFEFLPVEKNDGELTHCNGTTNTSVQDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVG 420

Query: 421 DILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTS 480
           DIL VTGFHNKAPQFKF+HRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTS
Sbjct: 421 DILKVTGFHNKAPQFKFIHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTS 480

Query: 481 YADTGSIPGHYVLFWELKKRGGN--ENLEEL-EVGLMEECCSSIEQSLDSVYRRCRSKDK 540
           YADT SIPGHYVLFWE+K+R G+  EN EEL EV LMEECCSSIE+SLDSVYRRCRSKDK
Sbjct: 481 YADTESIPGHYVLFWEMKRRSGSNYENFEELVEVDLMEECCSSIEESLDSVYRRCRSKDK 540

Query: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKT 600
           SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVV RYFS KT
Sbjct: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVARYFSKKT 600

Query: 601 PFWEPFRAHYSH 605
           PFWEPFR  YSH
Sbjct: 601 PFWEPFRGQYSH 612

[Close]

BLAST of Lsi07G003990 vs. ExPASy TrEMBL
Match: A0A6J1GWE7 (indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita moschata OX=3662 GN=LOC111457496 PE=3 SV=1)

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 544/604 (90.07%), Postives = 574/604 (95.03%), Query Frame = 0


Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNG-QSPDLQSF 60
           MLP FDPN+NE GLKLLEDLTQNA+QIQEQVIEKILTQN NTEYLK+F+NG  SPD+  F
Sbjct: 1   MLPSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 61  KNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KN+VPVVNYEDIKPYIERIANGEPS+IIS+QPI ELLTSSGTSGG PKMMPSTAEDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPIIELLTSSGTSGGHPKMMPSTAEDLDRK 120

Query: 121 TFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFN 180
           TFFYNLLVPV+NKYVDGLE+GKGMYLLFIKPE+STP GLMARPVLTSYYKSKNFRNRPFN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKEL 240
           RYNVYTSPDETILC+DS+QSMYCQLLCGL+QR++VLRVGAVFASAFLRAIKFLED+WKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQREDVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 241 SDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYI 300
           SDNIR+G VS+WITD NCRASVSL+LTKPNAALADLID MC EK WEGIIKKIWPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPKTKYI 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKP DVSYTLLPNMAFF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 361 EFLPVEKNDGELSHCNGSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILM 420
           EFLPVEKN+GELSHCNG SV   DEF+TVDLVDVKLG YYELVVTTFTGLYRYRVGDILM
Sbjct: 361 EFLPVEKNNGELSHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 420

Query: 421 VTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADT 480
           VTGFHN APQF+FVHRRNVVLSIDTDKTNEDDLLNAITKAKLL+EPLGVLLTEYTSYADT
Sbjct: 421 VTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTSYADT 480

Query: 481 GSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEI 540
           GSIPGHYVLFWELK RGG E+L ELE  LMEECCSS+E+SLDSVYRRCRSKDKSIGPLEI
Sbjct: 481 GSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEKSLDSVYRRCRSKDKSIGPLEI 540

Query: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEPFR 600
           RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAI+ILDSR VGR+FS KTPFWEPFR
Sbjct: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRAVGRFFSRKTPFWEPFR 600

Query: 601 AHYS 604
           A YS
Sbjct: 601 AQYS 604

[Close]

BLAST of Lsi07G003990 vs. ExPASy TrEMBL
Match: A0A0A0KVQ0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007100 PE=3 SV=1)

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 554/611 (90.67%), Postives = 576/611 (94.27%), Query Frame = 0


Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFL---NGQSPDLQ 60
           MLP FDPNNNESGLKLLEDLT NAKQIQEQVI+KIL QN NTEYLK+FL   +  S DLQ
Sbjct: 1   MLPNFDPNNNESGLKLLEDLTTNAKQIQEQVIQKILIQNSNTEYLKSFLHNHHSHSLDLQ 60

Query: 61  SFKNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLD 120
           +FK+SVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLD
Sbjct: 61  TFKHSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLD 120

Query: 121 RKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRP 180
           RKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTP GLMARPVLTSYYKSKNFRNRP
Sbjct: 121 RKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRNRP 180

Query: 181 FNRYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWK 240
           FN+YNVYTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLEDYWK
Sbjct: 181 FNKYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDYWK 240

Query: 241 ELSDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTK 300
           ELSDNIR GE+S+WI+D NCRASVS +LTK N  LADLIDG+C EKSWEGIIKK+WPKTK
Sbjct: 241 ELSDNIRKGELSQWISDPNCRASVSSVLTKSNPVLADLIDGLCGEKSWEGIIKKLWPKTK 300

Query: 301 YIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMA 360
           YIEVIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINFNPLSKP DVSYTLLPNMA
Sbjct: 301 YIEVIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPSDVSYTLLPNMA 360

Query: 361 FFEFLPVEKNDGELSHCNGSSVQSI-DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGD 420
           FFEFLPVEKNDGELSHCNG++  S+ +EFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGD
Sbjct: 361 FFEFLPVEKNDGELSHCNGTTNTSVQEEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGD 420

Query: 421 ILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSY 480
           IL VTGFHNKAPQFKF+HRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSY
Sbjct: 421 ILKVTGFHNKAPQFKFMHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSY 480

Query: 481 ADTGSIPGHYVLFWELKKRGGN--ENLEEL-EVGLMEECCSSIEQSLDSVYRRCRSKDKS 540
           ADT SIPGHYVLFWE+KKR G+  EN EEL EV LMEECCSSIE+SLDSVYRRCRSKDKS
Sbjct: 481 ADTESIPGHYVLFWEMKKRSGSNYENFEELVEVDLMEECCSSIEESLDSVYRRCRSKDKS 540

Query: 541 IGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTP 600
           IGPLEIRVVK GAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVV RYFS KTP
Sbjct: 541 IGPLEIRVVKQGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVARYFSKKTP 600

Query: 601 FWEPFRAHYSH 605
           FWEPFR  YSH
Sbjct: 601 FWEPFRGQYSH 611

[Close]

BLAST of Lsi07G003990 vs. NCBI nr
Match: XP_038900786.1 (indole-3-acetic acid-amido synthetase GH3.17-like [Benincasa hispida])

HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 568/614 (92.51%), Postives = 583/614 (94.95%), Query Frame = 0


Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFK 60
           MLPKFDP NNESGLKLLEDLTQNAKQIQEQVI+KILTQNFNTEYL TFLNG S DLQ+FK
Sbjct: 1   MLPKFDPTNNESGLKLLEDLTQNAKQIQEQVIQKILTQNFNTEYLNTFLNGHSLDLQTFK 60

Query: 61  NSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKT 120
           NSVPVVNYEDIKPYIERIANGEPS IISSQPITELLTSSGTSGGQPKMMPST EDLD KT
Sbjct: 61  NSVPVVNYEDIKPYIERIANGEPSQIISSQPITELLTSSGTSGGQPKMMPSTTEDLDNKT 120

Query: 121 FFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNR 180
           FFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTP GLMARPVLTSYYKSKNFRNRPFN+
Sbjct: 121 FFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRNRPFNK 180

Query: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELS 240
           YNVYTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLEDYWKELS
Sbjct: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDYWKELS 240

Query: 241 DNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIE 300
           DNIRNGE+S+WITD NCR +VS ILTKPNAALADLIDG+C EKSWEGIIKKIWPKTKYIE
Sbjct: 241 DNIRNGEISKWITDPNCRVAVSSILTKPNAALADLIDGLCCEKSWEGIIKKIWPKTKYIE 300

Query: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFE 360
           VIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFE
Sbjct: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFE 360

Query: 361 FLPVEKNDGELSHCNG-SSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILM 420
           FLPVEKNDGELSHCNG SSVQ+IDE KTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILM
Sbjct: 361 FLPVEKNDGELSHCNGRSSVQAIDELKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILM 420

Query: 421 VTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADT 480
           VTGFHN APQFKF+HRRNVVLSIDTDKTNEDDLLNAITKAKLL+EPLGVLLTEYTSYADT
Sbjct: 421 VTGFHNNAPQFKFIHRRNVVLSIDTDKTNEDDLLNAITKAKLLIEPLGVLLTEYTSYADT 480

Query: 481 GSIPGHYVLFWELKKRGGNENLEE---------LEVGLMEECCSSIEQSLDSVYRRCRSK 540
            SIPGHYV+FWELKKR GNENLEE         +EVGLMEECCSSIE+SLDSVYRRCRSK
Sbjct: 481 ESIPGHYVVFWELKKREGNENLEEENEEEEEEVVEVGLMEECCSSIEESLDSVYRRCRSK 540

Query: 541 DKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFST 600
           DKSIG LEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVV RYFS 
Sbjct: 541 DKSIGALEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVARYFSK 600

Query: 601 KTPFWEPFRAHYSH 605
           KTPFWEPF+  YSH
Sbjct: 601 KTPFWEPFKPQYSH 614

[Close]

BLAST of Lsi07G003990 vs. NCBI nr
Match: XP_022980842.1 (indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita maxima])

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 549/604 (90.89%), Postives = 575/604 (95.20%), Query Frame = 0


Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNG-QSPDLQSF 60
           ML  FDPN+NE GLKLLEDLTQNA+QIQEQVIEKILTQN NTEYLK+F+NG  SPD+  F
Sbjct: 1   MLSSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 61  KNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KN+VPVVNYEDIKPYIERIANGEPS+IIS+QPITELLTSSGTSGGQPKMMPSTAEDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120

Query: 121 TFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFN 180
           TFFYNLLVPV+NKYVDGLE+GKGMYLLFIKPE+STP GLMARPVLTSYYKSKNFRNRPFN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKEL 240
           RYNVYTSPDETILC+DS+QSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLED+WKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 241 SDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYI 300
           SDNIR+G VS+WITD NCRASVSL+LTKPNAALADLID MC EK WEGIIKKIWPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDSMCGEKLWEGIIKKIWPKTKYI 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKP DVSYTLLPNMAFF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 361 EFLPVEKNDGELSHCNGSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILM 420
           EFLPVEKN+GELSHCNG SV   DEF+TVDLVDVKLG YYELVVTTFTGLYRYRVGDILM
Sbjct: 361 EFLPVEKNNGELSHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 420

Query: 421 VTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADT 480
           VTGFHN APQF+FVHRRNVVLSIDTDKTNEDDLLNAITKAKLL+EPLGVLLTEYTSYADT
Sbjct: 421 VTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTSYADT 480

Query: 481 GSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEI 540
           GSIPGHYVLFWELK RGG E+L ELE  LMEECCSS+EQSLDSVYRRCRSKDKSIGPLEI
Sbjct: 481 GSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEQSLDSVYRRCRSKDKSIGPLEI 540

Query: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEPFR 600
           RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSR VGR+FS KTPFWEPFR
Sbjct: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRAVGRFFSRKTPFWEPFR 600

Query: 601 AHYS 604
           A YS
Sbjct: 601 AQYS 604

[Close]

BLAST of Lsi07G003990 vs. NCBI nr
Match: XP_008454158.1 (PREDICTED: indole-3-acetic acid-amido synthetase GH3.17-like [Cucumis melo] >KAA0044492.1 indole-3-acetic acid-amido synthetase GH3.17-like [Cucumis melo var. makuwa])

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 557/612 (91.01%), Postives = 578/612 (94.44%), Query Frame = 0


Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLN----GQSPDL 60
           MLP FDPNNNESGLKLLEDLT NAKQIQEQVI KILTQN NTEYLK+FL+      S DL
Sbjct: 1   MLPNFDPNNNESGLKLLEDLTTNAKQIQEQVIRKILTQNSNTEYLKSFLHNHHYSHSLDL 60

Query: 61  QSFKNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL 120
           Q+FK+SVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL
Sbjct: 61  QTFKHSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL 120

Query: 121 DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNR 180
           DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTP GLMARPVLTSYYKSKNFRNR
Sbjct: 121 DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRNR 180

Query: 181 PFNRYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYW 240
           PFN+YNVYTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLEDYW
Sbjct: 181 PFNKYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDYW 240

Query: 241 KELSDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKT 300
           KELSDNIR GE+S+WI+D NCRASVSLILTK N  LADLIDG+C EKSWEGIIKK+WPKT
Sbjct: 241 KELSDNIRKGELSQWISDPNCRASVSLILTKSNPVLADLIDGLCGEKSWEGIIKKLWPKT 300

Query: 301 KYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNM 360
           KYIEVIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINFNPLSKP DVSYTLLPNM
Sbjct: 301 KYIEVIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPSDVSYTLLPNM 360

Query: 361 AFFEFLPVEKNDGELSHCNGSSVQSI-DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVG 420
           AFFEFLPVEKNDGEL+HCNG++  S+ DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVG
Sbjct: 361 AFFEFLPVEKNDGELTHCNGTTNTSVQDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVG 420

Query: 421 DILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTS 480
           DIL VTGFHNKAPQFKF+HRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTS
Sbjct: 421 DILKVTGFHNKAPQFKFIHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTS 480

Query: 481 YADTGSIPGHYVLFWELKKRGGN--ENLEEL-EVGLMEECCSSIEQSLDSVYRRCRSKDK 540
           YADT SIPGHYVLFWE+K+R G+  EN EEL EV LMEECCSSIE+SLDSVYRRCRSKDK
Sbjct: 481 YADTESIPGHYVLFWEMKRRSGSNYENFEELVEVDLMEECCSSIEESLDSVYRRCRSKDK 540

Query: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKT 600
           SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVV RYFS KT
Sbjct: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVARYFSKKT 600

Query: 601 PFWEPFRAHYSH 605
           PFWEPFR  YSH
Sbjct: 601 PFWEPFRGQYSH 612

[Close]

BLAST of Lsi07G003990 vs. NCBI nr
Match: KAG6581972.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018391.1 Indole-3-acetic acid-amido synthetase GH3.17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1121.3 bits (2899), Expect = 0.0e+00
Identity = 547/604 (90.56%), Postives = 575/604 (95.20%), Query Frame = 0


Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNG-QSPDLQSF 60
           MLP FDPN+NE GLKLLEDLTQNA+QIQEQVIEKILTQN NTEYLK+F+NG  SPD+  F
Sbjct: 1   MLPSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 61  KNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KN+VPVVNYEDIKPYIERIANGEPS+IIS+QPITELLTSSGTSGG PKMMPSTAEDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGHPKMMPSTAEDLDRK 120

Query: 121 TFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFN 180
           TFFYNLLVPV+NKYVDGLE+GKGMYLLFIKPE+STP GLMARPVLTSYYKSKNFRNRPFN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKEL 240
           RYNVYTSPDETILC+DS+QSMYCQLLCGL+QRD+VLRVGAVFASAFLRAIKFLED+WKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQRDDVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 241 SDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYI 300
           SDNIR+G VS+WITD NCRASVSL+LTKPNAALADLID MC EK WEGIIKKIWPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPKTKYI 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKP DVSYTLLPNMAFF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 361 EFLPVEKNDGELSHCNGSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILM 420
           EFLPVEKN+GELSHCNG SV   DEF+TVDLVDVKLG YYELVVTTFTGLYRYRVGDILM
Sbjct: 361 EFLPVEKNNGELSHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 420

Query: 421 VTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADT 480
           VTGFHN APQF+FVHRRNVVLSIDTDKTNEDDLLNAITKAKLL+EPLGVLLTEYTSYADT
Sbjct: 421 VTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTSYADT 480

Query: 481 GSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEI 540
           GSIPGHYVLFWELK RGG E+L ELE  LMEECCSS+EQSLDSVYRRCRSKDKSIGPLEI
Sbjct: 481 GSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEQSLDSVYRRCRSKDKSIGPLEI 540

Query: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEPFR 600
           RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAI+ILDSR VGR+FS KTPFWEPFR
Sbjct: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRAVGRFFSRKTPFWEPFR 600

Query: 601 AHYS 604
           A YS
Sbjct: 601 AQYS 604

[Close]

BLAST of Lsi07G003990 vs. NCBI nr
Match: XP_023528388.1 (indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 546/604 (90.40%), Postives = 575/604 (95.20%), Query Frame = 0


Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNG-QSPDLQSF 60
           MLP FDPN++E GLKLLEDLTQNA+QIQEQVIEKILTQN NTEYLK+F+NG  SPD+  F
Sbjct: 1   MLPSFDPNDDEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 61  KNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KN+VPVVNYEDIKPYIERIANGEPS+IIS+QPITELLTSSGTSGG PKMMPSTAEDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGHPKMMPSTAEDLDRK 120

Query: 121 TFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFN 180
           TFFYNLLVPV+NKYVDGLE+GKGMYLLFIKPE+STP GLMARPVLTSYYKSKNFRNRPFN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKEL 240
           RYNVYTSPDETILC+DS+QSMYCQLLCGL+QRD+VLRVGAVFASAFLRAIKFLED+WKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQRDDVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 241 SDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYI 300
           SDNIR+G VS+WITD NCRASVSL+LTKPNAALADLID MC EK WEGIIKKIWPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPKTKYI 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKP DVSYTLLPNMAFF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 361 EFLPVEKNDGELSHCNGSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILM 420
           EFLPVEKN+GELSHCNG SV   DEF+TVDLVDVKLG YYELVVTTFTGLYRYRVGDILM
Sbjct: 361 EFLPVEKNNGELSHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 420

Query: 421 VTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADT 480
           VTGFHN APQF+FVHRRNVVLSIDTDKTNEDDLLNAITKAKLL+EPLGVLLTEYTSYADT
Sbjct: 421 VTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTSYADT 480

Query: 481 GSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEI 540
           GSIPGHYVLFWELK RGG E+L ELE  LMEECCSS+EQSLDSVYRRCRSKDKSIGPLEI
Sbjct: 481 GSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEQSLDSVYRRCRSKDKSIGPLEI 540

Query: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEPFR 600
           RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAI+ILDSR VGR+FS KTPFWEPFR
Sbjct: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRAVGRFFSRKTPFWEPFR 600

Query: 601 AHYS 604
           A YS
Sbjct: 601 AQYS 604

[Close]

BLAST of Lsi07G003990 vs. TAIR 10
Match: AT1G28130.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 892.1 bits (2304), Expect = 2.5e-259
Identity = 435/605 (71.90%), Postives = 515/605 (85.12%), Query Frame = 0


Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQS-PDLQSF 60
           M+P +DPN+ E+GLKLLEDLT NA+ IQ+QV+ +IL+QN  T+YL+ FL+G++  + QSF
Sbjct: 1   MIPSYDPNDTEAGLKLLEDLTTNAEAIQQQVLHQILSQNSGTQYLRAFLDGEADKNQQSF 60

Query: 61  KNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KN VPVVNY+D+KP+I+RIA+GE S I+S+QPITELLTSSGTS G+PK+MPSTAE+L+RK
Sbjct: 61  KNKVPVVNYDDVKPFIQRIADGESSDIVSAQPITELLTSSGTSAGKPKLMPSTAEELERK 120

Query: 121 TFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFN 180
           TFFY++LVP++NKYVDGL+EGKGMYLLFIKPE+ TP GLMARPVLTSYYKS++FRNRPFN
Sbjct: 121 TFFYSMLVPIMNKYVDGLDEGKGMYLLFIKPEIKTPSGLMARPVLTSYYKSQHFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKEL 240
           +YNVYTSPD+TILC DSKQSMYCQLLCGL+QR  VLRVGAVFASAFLRA+KFLED++KEL
Sbjct: 181 KYNVYTSPDQTILCQDSKQSMYCQLLCGLVQRSHVLRVGAVFASAFLRAVKFLEDHYKEL 240

Query: 241 SDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYI 300
             +IR G V+ WITDS+CR SV  IL  PN  LAD I+  C EKSWEGI+++IWPK KY+
Sbjct: 241 CADIRTGTVTSWITDSSCRDSVLSILNGPNQELADEIESECAEKSWEGILRRIWPKAKYV 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGIN NPL  P DVSYTLLPNMA+F
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINLNPLCDPADVSYTLLPNMAYF 360

Query: 361 EFLPV-EKNDGELSHCNGSSVQSID----EFKTVDLVDVKLGQYYELVVTTFTGLYRYRV 420
           EFLPV +K+  E+     S+    D    E   V+LV+V++GQYYE+V+TTFTGLYRYRV
Sbjct: 361 EFLPVDDKSHEEIHFATHSNTDDDDDALKEDLIVNLVNVEVGQYYEIVITTFTGLYRYRV 420

Query: 421 GDILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKL--LVEPLGVLLTE 480
           GDIL VTGFHNKAPQF+FV RRNVVLSIDTDKT+E+DLLNA+T+AKL  L  P  +LLTE
Sbjct: 421 GDILKVTGFHNKAPQFRFVQRRNVVLSIDTDKTSEEDLLNAVTQAKLNHLQHPSSLLLTE 480

Query: 481 YTSYADTGSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDK 540
           YTSYADT SIPGHYVLFWELK R  N+   +L+   ME+CCS +E  LD VYRRCR++DK
Sbjct: 481 YTSYADTSSIPGHYVLFWELKPRHSNDP-PKLDDKTMEDCCSEVEDCLDYVYRRCRNRDK 540

Query: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKT 598
           SIGPLEIRVV  G FD+LMDFCVSQGSS+NQYKTPRC+KS  A++ILDSRV+GR+FS + 
Sbjct: 541 SIGPLEIRVVSLGTFDSLMDFCVSQGSSLNQYKTPRCVKSGGALEILDSRVIGRFFSKRV 600

[Close]

BLAST of Lsi07G003990 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 708.4 bits (1827), Expect = 5.0e-204
Identity = 350/590 (59.32%), Postives = 452/590 (76.61%), Query Frame = 0


Query: 10  NESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTF-LNGQSPDLQSFKNSVPVVNY 69
           N+  L+L+E+LT NA Q+Q QV+E+ILT+N + EYL+   LNG++ D ++FKN +PV+ Y
Sbjct: 21  NKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRT-DRETFKNIMPVITY 80

Query: 70  EDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVP 129
           EDI+P I RIANG+ S I+SS+PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+LL+P
Sbjct: 81  EDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMP 140

Query: 130 VLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPD 189
           V++++V GLE GKGMY LFIK E  TP GL ARPVLTSYYKS +F+ RP++ Y  YTSP+
Sbjct: 141 VMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPN 200

Query: 190 ETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEV 249
           ETILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE +W EL  +IR G +
Sbjct: 201 ETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTL 260

Query: 250 SEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMA 309
           S  ITD + R +V+ IL KP+  LAD ++  C + SW+GII ++WP TKY++VIVTG+M+
Sbjct: 261 SSLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMS 320

Query: 310 QYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKND 369
           QYIPTL++YS GLPLV TMYASSECYFG+N  PL KP +VSYTL+P+MA+FEFLPV +N+
Sbjct: 321 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNN 380

Query: 370 GELSHCN-GSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKA 429
           G  +  N   ++   ++ + VDLVDVKLGQ YELVVTT+ GL RYRVGD+L VTGF NKA
Sbjct: 381 GVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNKA 440

Query: 430 PQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTGSIPGHYV 489
           PQF F+ R+NVVLSID+DKT+E +L NA+  A   + P    L+EYTSYADT SIPGHYV
Sbjct: 441 PQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYV 500

Query: 490 LFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAF 549
           LFWEL   G       +   + E+CC ++E+S ++VYR+ R  DKSIGPLEI++V+ G F
Sbjct: 501 LFWELCLDGNT----PIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTF 560

Query: 550 DALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEP 598
           D LMD+ +S G+S+NQYKTPRC+K    I++L+SRVV  YFS K P W P
Sbjct: 561 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604

[Close]

BLAST of Lsi07G003990 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 705.7 bits (1820), Expect = 3.3e-203
Identity = 347/590 (58.81%), Postives = 450/590 (76.27%), Query Frame = 0


Query: 10  NESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTF-LNGQSPDLQSFKNSVPVVNY 69
           N++ L+ +ED+T NA  +Q +V+E+IL++N + EYLK   L G++ D ++FK+ +PVV Y
Sbjct: 21  NKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRT-DRETFKHIMPVVTY 80

Query: 70  EDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVP 129
           EDI+P I RIANG+ S ++ S PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+LL+P
Sbjct: 81  EDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMP 140

Query: 130 VLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPD 189
           V++++V GL++GKGMY LFIK E  TP GL ARPVLTSYYKS +F+NRP++ Y  YTSP+
Sbjct: 141 VMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPN 200

Query: 190 ETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEV 249
           +TILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE +W EL+ +IR G +
Sbjct: 201 QTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTL 260

Query: 250 SEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMA 309
           S  ITDS+ R +V  IL KP+  LAD ++  C + SW+GII ++WP TKY++VIVTG+M+
Sbjct: 261 SSEITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMS 320

Query: 310 QYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKND 369
           QYIPTL++YS GLPLV TMYASSECYFG+N  PL KP +VSYTL+PNMA+FEFLPV +N 
Sbjct: 321 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNS 380

Query: 370 GELSHCN-GSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKA 429
           G  S  +   ++   ++ + VDLVDVKLGQ YELVVTT+ GLYRYRVGD+L V GF N A
Sbjct: 381 GVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNA 440

Query: 430 PQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTGSIPGHYV 489
           PQF F+ R+NVVLSID+DKT+E +L NA+  A   + P    L+EYTSYADT SIPGHYV
Sbjct: 441 PQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYV 500

Query: 490 LFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAF 549
           LFWEL   G       +   + E+CC +IE+SL+SVYR+ R  DKSIGPLEI++V+ G F
Sbjct: 501 LFWELCLNGNT----PIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTF 560

Query: 550 DALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEP 598
           D LMD+ +S G+S+NQYKTPRC+K    I++L+SRVV  YFS K P W P
Sbjct: 561 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

[Close]

BLAST of Lsi07G003990 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 694.9 bits (1792), Expect = 5.8e-200
Identity = 338/586 (57.68%), Postives = 442/586 (75.43%), Query Frame = 0


Query: 14  LKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFKNSVPVVNYEDIKP 73
           LK +E++T+N   +QE+V+ +ILT+N NTEYLK F      D ++FK+ VPVV YED+KP
Sbjct: 24  LKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVTYEDLKP 83

Query: 74  YIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVPVLNKY 133
            I+RI+NG+ S I+SS PITE LTSSGTS G+ K+MP+  EDLDR+   Y+LL+PV+N Y
Sbjct: 84  EIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLMPVMNLY 143

Query: 134 VDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDETILC 193
           V GL++GKG+Y LF+K E  T  GL ARPVLTSYYKS +F+ RP++ YNVYTSP+E ILC
Sbjct: 144 VPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSPNEAILC 203

Query: 194 SDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEVSEWIT 253
           SDS QSMY Q+LCGLL R EVLR+GAVFAS  LRAI FL++ WKEL+ +I  G +S  I 
Sbjct: 204 SDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGTLSSRIF 263

Query: 254 DSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMAQYIPT 313
           D   +  +S ILTKP+  LA+ + G+C +++WEGII KIWP TKY++VIVTG+MAQYIPT
Sbjct: 264 DPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAMAQYIPT 323

Query: 314 LEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKNDGELSH 373
           LE+YSGGLP+  TMYASSE YFGIN  P+ KP +VSYT++PNMA+FEFLP   +DG+   
Sbjct: 324 LEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP-HNHDGD--- 383

Query: 374 CNGSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKAPQFKFV 433
             G++  S+DE   V+L +V++G+ YELV+TT+ GLYRYRVGDIL VTGFHN APQFKF+
Sbjct: 384 --GAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFI 443

Query: 434 HRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTGSIPGHYVLFWELK 493
            R+NV+LS+++DKT+E +L  A+  A  L    G  + EYTSYA+T +IPGHYV++WEL 
Sbjct: 444 RRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWELL 503

Query: 494 KRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAFDALMDF 553
            R  +  L   EV  M +CC  +E+SL+SVYR+ R  DKSIGPLEIRVV++G F+ LMD+
Sbjct: 504 GRDQSNALMSEEV--MAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELMDY 563

Query: 554 CVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEPFR 600
            +S+G+S+NQYK PRC+     +++LDSRVV  +FS   P W P R
Sbjct: 564 AISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPER 601

[Close]

BLAST of Lsi07G003990 vs. TAIR 10
Match: AT1G28130.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 688.7 bits (1776), Expect = 4.1e-198
Identity = 338/462 (73.16%), Postives = 387/462 (83.77%), Query Frame = 0


Query: 143 MYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDETILCSDSKQSMYC 202
           MYLLFIKPE+ TP GLMARPVLTSYYKS++FRNRPFN+YNVYTSPD+TILC DSKQSMYC
Sbjct: 1   MYLLFIKPEIKTPSGLMARPVLTSYYKSQHFRNRPFNKYNVYTSPDQTILCQDSKQSMYC 60

Query: 203 QLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEVSEWITDSNCRASVS 262
           QLLCGL+QR  VLRVGAVFASAFLRA+KFLED++KEL  +IR G V+ WITDS+CR SV 
Sbjct: 61  QLLCGLVQRSHVLRVGAVFASAFLRAVKFLEDHYKELCADIRTGTVTSWITDSSCRDSVL 120

Query: 263 LILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMAQYIPTLEFYSGGLP 322
            IL  PN  LAD I+  C EKSWEGI+++IWPK KY+EVIVTGSMAQYIPTLEFYSGGLP
Sbjct: 121 SILNGPNQELADEIESECAEKSWEGILRRIWPKAKYVEVIVTGSMAQYIPTLEFYSGGLP 180

Query: 323 LVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPV-EKNDGELSHCNGSSVQS 382
           LVSTMYASSECYFGIN NPL  P DVSYTLLPNMA+FEFLPV +K+  E+     S+   
Sbjct: 181 LVSTMYASSECYFGINLNPLCDPADVSYTLLPNMAYFEFLPVDDKSHEEIHFATHSNTDD 240

Query: 383 ID----EFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKAPQFKFVHRRN 442
            D    E   V+LV+V++GQYYE+V+TTFTGLYRYRVGDIL VTGFHNKAPQF+FV RRN
Sbjct: 241 DDDALKEDLIVNLVNVEVGQYYEIVITTFTGLYRYRVGDILKVTGFHNKAPQFRFVQRRN 300

Query: 443 VVLSIDTDKTNEDDLLNAITKAKL--LVEPLGVLLTEYTSYADTGSIPGHYVLFWELKKR 502
           VVLSIDTDKT+E+DLLNA+T+AKL  L  P  +LLTEYTSYADT SIPGHYVLFWELK R
Sbjct: 301 VVLSIDTDKTSEEDLLNAVTQAKLNHLQHPSSLLLTEYTSYADTSSIPGHYVLFWELKPR 360

Query: 503 GGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAFDALMDFCV 562
             N+   +L+   ME+CCS +E  LD VYRRCR++DKSIGPLEIRVV  G FD+LMDFCV
Sbjct: 361 HSNDP-PKLDDKTMEDCCSEVEDCLDYVYRRCRNRDKSIGPLEIRVVSLGTFDSLMDFCV 420

Query: 563 SQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEP 598
           SQGSS+NQYKTPRC+KS  A++ILDSRV+GR+FS + P WEP
Sbjct: 421 SQGSSLNQYKTPRCVKSGGALEILDSRVIGRFFSKRVPQWEP 461

The following BLAST results are available for this feature:

BLAST of Lsi07G003990 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FZ87	3.5e-258	71.90	Indole-3-acetic acid-amido synthetase GH3.17 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
O81829	7.1e-203	59.32	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q9LSQ4	4.6e-202	58.81	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
P0C0M3	2.6e-197	58.00	Probable indole-3-acetic acid-amido synthetase GH3.11 OS=Oryza sativa subsp. jap...	[more]
Q8GZ29	3.8e-196	56.74	Indole-3-acetic acid-amido synthetase GH3.15 OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Lsi07G003990 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ISD7	0.0e+00	90.89	indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A5A7TMG2	0.0e+00	91.01	Indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo var. makuwa OX...	[more]
A0A1S3BYQ7	0.0e+00	91.01	indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A6J1GWE7	0.0e+00	90.07	indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A0A0KVQ0	0.0e+00	90.67	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007100 PE=3 SV=1	[more]

BLAST of Lsi07G003990 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900786.1	0.0e+00	92.51	indole-3-acetic acid-amido synthetase GH3.17-like [Benincasa hispida]	[more]
XP_022980842.1	0.0e+00	90.89	indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita maxima]	[more]
XP_008454158.1	0.0e+00	91.01	PREDICTED: indole-3-acetic acid-amido synthetase GH3.17-like [Cucumis melo] >KAA...	[more]
KAG6581972.1	0.0e+00	90.56	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_023528388.1	0.0e+00	90.40	indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi07G003990 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G28130.1	2.5e-259	71.90	Auxin-responsive GH3 family protein	[more]
AT4G27260.1	5.0e-204	59.32	Auxin-responsive GH3 family protein	[more]
AT5G54510.1	3.3e-203	58.81	Auxin-responsive GH3 family protein	[more]
AT4G37390.1	5.8e-200	57.68	Auxin-responsive GH3 family protein	[more]
AT1G28130.2	4.1e-198	73.16	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 10..37
None	No IPR available	PANTHER	PTHR31901:SF33	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.17	coord: 3..595
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 17..573 e-value: 2.9E-203 score: 676.5
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 3..595

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi07G003990.1	Lsi07G003990.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016881	acid-amino acid ligase activity