Lsi07G003890 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G003890
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionUbiquitin thioesterase
Locationchr07: 4169903 .. 4176211 (-)
RNA-Seq ExpressionLsi07G003890
SyntenyLsi07G003890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACAAGGCACGATTGAGATTCAGATTCAGATTCATCGGAATTTTTAGTTTCAGTTTCACCATAGTTGACCTCTTTTCATCTTCTTCTGCAGCCCTAATCCGTCTCGCCACCGTCCACTCCGCTTTCTCCCTCATTTCCATTCCGACGCTTCCTCCATTATCACTTACTGCAACAATGGTTATCTCGCTCCTCCATGGCGCCCTTTTCCCTTTCCTCTTTTAGTATCTGTTTCCCCCAAGCACTGACAAGTGAGCACTGAGCGTCCTTCATTGCCCTCAAGCTCTTCAATCTCCATCTCTCGTCTACCTCTTAATCTGTAAGATTCTTTCTCTTTGTCTCTCCTATTTGCTCCTTGCTTAGTATGGTTTTGTTGGATCATTATCGGATCTGGATTCTGATTGACCGGTACTAGTATGGATGGATTTAAGTTTCGATGCTTGCATCGCTTTCTTGGTTGTAGCCTCTATGCGTGGGAAGTATTTTTGTGTCTACGAGTTTATATGCTTACCGAATATGTTGAGGGAATTTAATAATCTTGGACTTTTTTTTCTTTTCTTTTCTTAAATCAAGTTCGAATTAATGATTTATTACTTTCATTTCCAAGTTTTGGGGAAATTAAAGAAGTAATTTTAAGGTCCTGTTGTTTGCCCTAGTTTCTGATGCGAAGTGCTCAAGCTAATCACTAATGCACAGTGAGAAGTAATGACTCAAGATATGAAGTTTCTATCTCGATGTTATGTTTCATTTTGATGAGCGATTTGGAACCTGATTTTTTTTCCCTTTCCTTTATAACTACGAGCACTTTCCAGTTCTTTAGTAGTATAGCCTACTTTCTTGCAACATGATCTTTAAGAACTCGGCTAAGAAATTGGATGTCGACTGTATTAGGTTTTTAAACATTGGATTTATAGATGTGCTTGTTAAAAGTGTGGAATAAGTAATTGCGCTGGTCTGATATTGATATGCAATGTTTTCTTAATAACTGCTGTGGTCTATATTCACTGGCATTCAATTATCCGTATCATATGGTTTGAGAATGATCGAGTTATTAACTTTAAAGATGAGAATAAATATGCTATTGCTACACTTTTTTTTTTTTTTTTTTTTTTTACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTGCTACACATTTATTTATTACTAGATAATGTTTCTTGAACGCTCAGCAGGTGTTCTCCTAGCAGGCAAGTTTATGAGCTGGTTGGATCATCACAGTGAAGTTACAAGGGAAATTCTTGGATAGGTTTTTCAAACCCGACTTCACTTACTGAGGAAACAAAGTATTTGTATTCCTTTAAAAGAATGCAGAACCAGGAAAATGTTGATGCAGATGGGAAAGCGGAGTCTGTAATATCTATTCAAACATCAGAGTACGATAGTTGGGGCAATTTGGGTGATGATGATATCATGCTGCAGAAATCTGCAATTTTTGTTCAGGAAGCTGAGAAAGTTCCATTTGTTGGTGACAAGGCATGTTTCACCTAACTAAGTTTTAATCTCCTGCATTTTCCTGTCTTGATCTTGCCACACTTTTCCCTCCCTTTCTTCCTTTTGTTTGTGTTATCTATGATTCTTTGCATGTAGGCAACCAAGTTTTACTTTTTAGTCTTCCCTTCTTTTCTCATAAATTATGCTATGAAGTAGTGCTCTTATTTTATTTGTTATAACATGTTTGGGTAACTCCCAAAATGTCGTCTAGCAACACAGTTGCTAATGTCTAACATATGTTTGGAAATTATTTGGAACTTAGAAATGTTGGAGGGATTCTGCATCGCTTTTTGCTTCAAAAGCCACCCTTTCTGTATGTTTAATGGCATTTTTGGTGTCTAACCTTTGGGCCTACGTATCTAACCCTTTTTGTAATGTTACTATTTGTTTTTAATTCTTATTTTAACAATTGGTGGTCTATCTTGTTAGTTCTTTTTTCCCCCTACTTTATTTTGGCTTGGCTTGGCTCTATTTTTGCATTTTTATTTATCAAAAGTGAACCTTTATATCTCTGTTTTTTTTCCATTTTTACTCATTTTGGAGTTTGTATCCTTCGAGCATTTGTTTCTCATTTTGTTTTTCATTTTTTTAATTGTATGTTTTGAATGCCTTACAACTGTGGTTTTATAGCTCTAAGATGTTTTTATCAGTTGATTTTTCTTTTACTCCATAATTAAACTTAATGGCAATGGTGTTGCAGGAACCACTTTCTTCTTTAGAAGCTGAATATAAATCCGGAAGTCCCATTTTGCTGGAGAAAATTAAGGTTCTATTTGTTGCTTATTGTTATTTTAGTAGAGTTATTGTCCTTGGAATAATTATTTATGTTAAATGTTACATGTTATGCAGGTGCTCAGTGGCCAATATGCTGCCATTCGTCGAACACGTGGTGATGGGAACTGCTTTTTCCGCAGCTTTATGTTTTCGTATCTTGTAAGTCTAATTCTTTTAATTAATCTCAGCAGCTTGATTATTGTACTTTTTGTGCATCATCGAGCATTGGCTGTTACTGACTTGGCCTCTCAAATCAGGAACATATTTTAGAGTCTCAAGACAGAACTGAAGTTGATCGTATCAAAACAAATGTGGAAAATTGTAGGAAAACACTTCGAAGTCTGGGATATACAGAATTTACTTTCGAAGATTTTTTTGCGGTAAATTGTTACTATTCCCTTTTTAAAATTAATTTATTATACATATATCACCCTCTGCTTTAAGCAGTATGGCTTATTTCTCCCGTTTAACGGGAGGCAGTGGAAGTTGTGAACTTCTTCCTAGTCTCTAAATGTGTTATTGAACATAGCAGATAGAAATATCGCTACAACAAGTTTATTGTTGCCGAGATGTTTTCTTTGCAATGTGTGATGCTAGAGTAGGTTTATTCTCGAGCTTGTTTATGGCTTAAATTTGAGCGACTTGTCAAGGAGTTACACTGATGCAGATATGCATAATTTTGCCAATAGGGAGTCTTTAACCTTGACCTTGTGGGGATACCACATCTTACCTTCTTTTGAATTGGGTTTTATGTTAATATAATTCATTAACTCTCAGATTCGCATAAATAATTGTGTTTGCTCTTTCCTCGTGTTTATCAAGGGGGATTGTGCTCCTGGATTATTGGTTTAAGGAGCCTCAGAAAGCTTGTGGTCAAGTGTTTTACTTGCTTAGCTAGGTTTTACACCTTGCCTCATTGACTTTGTTTCAAAAAGACCCCAATTGGTTTATGATTTTTATCATTTAAAAATATACTATGGTCAAATGAGTAAATAAAGAAAAAATCAAACAAAACGTTTGCTGTTTTACTTTATTGAACCTTTTCACCTAAGGATGTGCTCTTTCTCTCTGATTGATGGATAATTGGATAGGAATGAGGAAATTAGACCAAACTAAAGAGGAAACTATGGAGGGTTGCAAGTTTAATGATAGAAAAAGTGGCGATGATAATCTATTATTGCGATGACAGATTTTAACTTTAACATCTTTACAAATTTGCAAGGACATTTGTAGTTTTTGTAATTGAATTTATTTGTTTTTGTTCTCTATCTCATAGTTCATGCTAACTTTTAATATATGAACATTTTATATATTGTGTCTTCACCTCATTGAAGTCCTGATTCTTTTTTGTTACTCAGTCACATATAAACTCTAAAGAGCTATTCTGCTTCAGTGTGTTTGCCACTTCTATAAACATTGTCCCAGATTTATATCACCTTAAAGATGTCTAGAGTCTAGAGTCTAGACTCATCCTTCTTTATTATTGCCTTCTTTATTTCATTTCGGTATAAACTAGCTAGATATCCTTGGTGGTTAATGATGGATGCCTTAAATATAAATTTTTTATTAATAAATATGTTTAAAATATCTTTACAGCTATTCCTTGAGCAGCTGGAAAGTGCTCTTCAAGGAAATGAAACTTCTATAAGGTGTCAATAACCTGCCCTTCTACCTTGGAATTTTGATTCCCGAATTTTTATTTTATAGTGGAATCTTCTGTTGCCATCGATTATGAAATTCTGCCATTCATGATTGAATTTGACTCTTATTTTTCTTGGATGCAGTCATGATGAACTTGTAACTAGGAGTCGGGACCAGTCTATCTCTGATTATGGTCAGTGATGTTTGTACATTCAATATCCTATTGCCTCTTTCTGTTTCTACTGACAAAATTGTTTTGCTAGTTGTTATGTTTTTCAGGTTTGTTACATCAGGTGAAATACAAAAGCGTTCGGAATTTTTTGAGCCTTTTATTCTGGGATTGACAAATGGCACAGTTGATCAGGTTTGTTAAATTTATAATAGCGTCTACAAGTTTATCATTATGTGTTGACTTTTATCTTCAGATTTATGTTGGAAATTTAACCATACAAGTCATTTGAAATCTAAGTTGATTTGGTTAGGGTGACCCTTTTGTAAGTGTAGTCTCGAGAAGAAGCAGTTAATAGTTAATGTGCAATAATGATGATGTTAGACCATGTATCCTTGGACAGGAATAAGAATGGATCTTCCCGATTTCCATTCTTGTTGTTTACTTCATTTGGGGAATAGAATAAGTACTACCCACTTAAAAATGGAAAACCGTGCTCTTAGTTTTTAGCAACATCTTTACTTCACAAAAGATAATCATTCTCCTATTGCTGTTCTTTGGGTTCAATTTCTTTTCCTAAATCTCTACTTTTTTGTGGGTCTTGCATTTGTTGAACTTTGATTCTCATTCTAAGAATCTGAATGTTAACCAACAATATCAACCACAATTTTATTCTTACTGATCCAGATTTCATAGTAATTTTAGTAAACAAAGTATTTAAAATTTTATTTCAAATCCATAATTTTTCTATCCAATCAGTCGTGATGATTCTTGTACGGTTTTATTCCCATTAAATAAACCATGCCTTAGGTTAATTATTTTATTATCGCATCTATGTTCAAATGAAATCACTTCAGCCTTGTTGTTTTCCCTTTTTTGAGGTCACGTGGGGGATTCGAATTTCTAACCTTTTGGTCGGCTATTATTTCCTTTTTTGTGCATCAGAATCCCAAACAAACAAACAGTAAGCCTTTGGTCTTTATGTTTGGGCGTTAAATTTTCATTTTTAGTTTTCTCTTTTTTGTGGGGAAATTTTGTTGGGCGTATATTAGCTTTCTAAGATGTCAATGGACATTTAATTTCTTATTTAAGAAGAAAGAGAACCTGTTTATCATGAATTCCAATCTCATTCTCTTTGGCACAGTTCTGCAAATCAGCAGTTGAACCAATGGGTGAAGAGAGTGATCATGTACACATAATTGCCTTATCTGATGCTTTGGGTGTGGCGATTCGTGTTCTGTACCTCGATCGGAGCTCTTGTGATTCTGGTGGCCTCAGTGTAAATCACCATGATTTTGTTCCTGCTGCTAGTGAGGTCTCAAGTGATAGTGGTGCTGGCTCTGAGATTAAGATCCCTTTCATCACTTTGCTCTATCGTCCCGGTCATTATGATATCCTTTATCCAAAGTGATGGTAAACAGCTCTCATGAACTTATTTGAAAAATTGCCACAGAAATGTCTCTTCCTCCCACCTTTTATATGCAACAGATTAACTTTATAGTCTGTATAAAATGATTATGTTTAAAAGTATATTGTCGCTGCCATCCTCATTCCTCATGCAAAATGATATTTTCATCTGTAGTTGTCTACCATTATTTGCAAATTTGTTTCATGTCTTTGATTTACTTGGGAATTTTATTCAGTGTTGGAGGTTTTTTGTTCTGGATGGTTCAGCTTGAATTTTATGTCATGTATTTCAGGCCATTGGCAAACCCTGGTCGGGCTCTCAGAGTTGAACTGCATTGACATGACAGACATGGATCCAAGCTTCAACAAGATAAAGGTTGGATTTCATAATTGATGCTTGCCAGATGTTCTGTACTGATTACTGATGAGCTGTTTGAGTAT

mRNA sequence

AAACAAGGCACGATTGAGATTCAGATTCAGATTCATCGGAATTTTTAGTTTCAGTTTCACCATAGTTGACCTCTTTTCATCTTCTTCTGCAGCCCTAATCCGTCTCGCCACCGTCCACTCCGCTTTCTCCCTCATTTCCATTCCGACGCTTCCTCCATTATCACTTACTGCAACAATGGTTATCTCGCTCCTCCATGGCGCCCTTTTCCCTTTCCTCTTTTAGTATCTGTTTCCCCCAAGCACTGACAAGTGAGCACTGAGCGTCCTTCATTGCCCTCAAGCTCTTCAATCTCCATCTCTCGTCTACCTCTTAATCTCAGGTGTTCTCCTAGCAGGCAAGTTTATGAGCTGGTTGGATCATCACAGTGAAGTTACAAGGGAAATTCTTGGATAGGTTTTTCAAACCCGACTTCACTTACTGAGGAAACAAAGTATTTGTATTCCTTTAAAAGAATGCAGAACCAGGAAAATGTTGATGCAGATGGGAAAGCGGAGTCTGTAATATCTATTCAAACATCAGAGTACGATAGTTGGGGCAATTTGGGTGATGATGATATCATGCTGCAGAAATCTGCAATTTTTGTTCAGGAAGCTGAGAAAGTTCCATTTGTTGGTGACAAGGAACCACTTTCTTCTTTAGAAGCTGAATATAAATCCGGAAGTCCCATTTTGCTGGAGAAAATTAAGGTGCTCAGTGGCCAATATGCTGCCATTCGTCGAACACGTGGTGATGGGAACTGCTTTTTCCGCAGCTTTATGTTTTCGTATCTTGAACATATTTTAGAGTCTCAAGACAGAACTGAAGTTGATCGTATCAAAACAAATGTGGAAAATTGTAGGAAAACACTTCGAAGTCTGGGATATACAGAATTTACTTTCGAAGATTTTTTTGCGCTATTCCTTGAGCAGCTGGAAAGTGCTCTTCAAGGAAATGAAACTTCTATAAGTCATGATGAACTTGTAACTAGGAGTCGGGACCAGTCTATCTCTGATTATGGTCAGTTTGTTACATCAGGTGAAATACAAAAGCGTTCGGAATTTTTTGAGCCTTTTATTCTGGGATTGACAAATGGCACAGTTGATCAGTTCTGCAAATCAGCAGTTGAACCAATGGGTGAAGAGAGTGATCATGTACACATAATTGCCTTATCTGATGCTTTGGGTGTGGCGATTCGTGTTCTGTACCTCGATCGGAGCTCTTGTGATTCTGGTGGCCTCAGTGTAAATCACCATGATTTTGTTCCTGCTGCTAGTGAGGTCTCAAGTGATAGTGGTGCTGGCTCTGAGATTAAGATCCCTTTCATCACTTTGCTCTATCGTCCCGGTCATTATGATATCCTTTATCCAAAGTGATGGCCATTGGCAAACCCTGGTCGGGCTCTCAGAGTTGAACTGCATTGACATGACAGACATGGATCCAAGCTTCAACAAGATAAAGGTTGGATTTCATAATTGATGCTTGCCAGATGTTCTGTACTGATTACTGATGAGCTGTTTGAGTAT

Coding sequence (CDS)

ATGCAGAACCAGGAAAATGTTGATGCAGATGGGAAAGCGGAGTCTGTAATATCTATTCAAACATCAGAGTACGATAGTTGGGGCAATTTGGGTGATGATGATATCATGCTGCAGAAATCTGCAATTTTTGTTCAGGAAGCTGAGAAAGTTCCATTTGTTGGTGACAAGGAACCACTTTCTTCTTTAGAAGCTGAATATAAATCCGGAAGTCCCATTTTGCTGGAGAAAATTAAGGTGCTCAGTGGCCAATATGCTGCCATTCGTCGAACACGTGGTGATGGGAACTGCTTTTTCCGCAGCTTTATGTTTTCGTATCTTGAACATATTTTAGAGTCTCAAGACAGAACTGAAGTTGATCGTATCAAAACAAATGTGGAAAATTGTAGGAAAACACTTCGAAGTCTGGGATATACAGAATTTACTTTCGAAGATTTTTTTGCGCTATTCCTTGAGCAGCTGGAAAGTGCTCTTCAAGGAAATGAAACTTCTATAAGTCATGATGAACTTGTAACTAGGAGTCGGGACCAGTCTATCTCTGATTATGGTCAGTTTGTTACATCAGGTGAAATACAAAAGCGTTCGGAATTTTTTGAGCCTTTTATTCTGGGATTGACAAATGGCACAGTTGATCAGTTCTGCAAATCAGCAGTTGAACCAATGGGTGAAGAGAGTGATCATGTACACATAATTGCCTTATCTGATGCTTTGGGTGTGGCGATTCGTGTTCTGTACCTCGATCGGAGCTCTTGTGATTCTGGTGGCCTCAGTGTAAATCACCATGATTTTGTTCCTGCTGCTAGTGAGGTCTCAAGTGATAGTGGTGCTGGCTCTGAGATTAAGATCCCTTTCATCACTTTGCTCTATCGTCCCGGTCATTATGATATCCTTTATCCAAAGTGA

Protein sequence

MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLSSLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDRIKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISDYGQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDALGVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDILYPK
Homology
BLAST of Lsi07G003890 vs. ExPASy Swiss-Prot
Match: Q8LG98 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 OS=Arabidopsis thaliana OX=3702 GN=OTU1 PE=1 SV=2)

HSP 1 Score: 412.9 bits (1060), Expect = 3.0e-114
Identity = 211/303 (69.64%), Postives = 241/303 (79.54%), Query Frame = 0

Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ ++  D +AE   SI   + + WGN    +    + +   +EA KVP+VGDKEPLS
Sbjct: 1   MQNQIDMVKD-EAEVAASISAIKGEEWGNCSSVE---DQPSFQEEEAAKVPYVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SL AEY+SGSPILLEKIK+L  QY  IRRTRGDGNCFFRSFMFSYLEHILESQDR EVDR
Sbjct: 61  SLAAEYQSGSPILLEKIKILDSQYIGIRRTRGDGNCFFRSFMFSYLEHILESQDRAEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IK NVE CRKTL++LGYT+FTFEDFFALFLEQL+  LQG E SIS+DELV RSRDQS+SD
Sbjct: 121 IKVNVEKCRKTLQNLGYTDFTFEDFFALFLEQLDDILQGTEESISYDELVNRSRDQSVSD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVT+G+I+ R++FFEPFI GL+N TVDQFCKS+VEPMGEESDH+HI ALSDAL
Sbjct: 181 YIVMFFRFVTAGDIRTRADFFEPFITGLSNATVDQFCKSSVEPMGEESDHIHITALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GVAIRV+YLDRSSCDSGG++VNHHDFVP       D     E   PFITLLYRPGHYDIL
Sbjct: 241 GVAIRVVYLDRSSCDSGGVTVNHHDFVPVGITNEKD----EEASAPFITLLYRPGHYDIL 295

BLAST of Lsi07G003890 vs. ExPASy Swiss-Prot
Match: Q96FW1 (Ubiquitin thioesterase OTUB1 OS=Homo sapiens OX=9606 GN=OTUB1 PE=1 SV=2)

HSP 1 Score: 181.0 bits (458), Expect = 1.9e-44
Identity = 116/270 (42.96%), Postives = 155/270 (57.41%), Query Frame = 0

Query: 32  DDDIMLQKSAIFVQEAEKVPFVGDKEPLSSLEAEYKSGSPILLEKIKVLSGQYAAIRRTR 91
           D+ IM Q+  I  + A + P V ++  LS L  EY     I  +KIK L  +Y+ IR+TR
Sbjct: 27  DEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTR 86

Query: 92  GDGNCFFRSFMFSYLEHILESQDRTEVDRIKTNVENCRKTLRSLGYTEFTFEDFFALFLE 151
            DGNCF+R+F FS+LE +L+  D  E+ R K      ++ L S G+TEFT EDF   F++
Sbjct: 87  PDGNCFYRAFGFSHLEALLD--DSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMD 146

Query: 152 QLESALQGNETSISHDELVTRSRDQSISD----YGQFVTSGEIQKRSEFFEPFILGLTNG 211
            +E      E   S  +L+    DQS SD    Y + +TSG +Q+ S+FFE FI G    
Sbjct: 147 LIEQV----EKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEG--GR 206

Query: 212 TVDQFCKSAVEPMGEESDHVHIIALSDALGVAIRVLYLDRSSCDSGGLSVNHHDFVPAAS 271
           TV +FC+  VEPM +ESDH+HIIAL+ AL V+I+V Y+DR      G + N H F P  S
Sbjct: 207 TVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGE----GGTTNPHIF-PEGS 266

Query: 272 EVSSDSGAGSEIKIPFITLLYRPGHYDILY 298
           E             P + LLYRPGHYDILY
Sbjct: 267 E-------------PKVYLLYRPGHYDILY 270

BLAST of Lsi07G003890 vs. ExPASy Swiss-Prot
Match: Q7TQI3 (Ubiquitin thioesterase OTUB1 OS=Mus musculus OX=10090 GN=Otub1 PE=1 SV=2)

HSP 1 Score: 181.0 bits (458), Expect = 1.9e-44
Identity = 116/270 (42.96%), Postives = 155/270 (57.41%), Query Frame = 0

Query: 32  DDDIMLQKSAIFVQEAEKVPFVGDKEPLSSLEAEYKSGSPILLEKIKVLSGQYAAIRRTR 91
           D+ IM Q+  I  + A + P V ++  LS L  EY     I  +KIK L  +Y+ IR+TR
Sbjct: 27  DEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTR 86

Query: 92  GDGNCFFRSFMFSYLEHILESQDRTEVDRIKTNVENCRKTLRSLGYTEFTFEDFFALFLE 151
            DGNCF+R+F FS+LE +L+  D  E+ R K      ++ L S G+TEFT EDF   F++
Sbjct: 87  PDGNCFYRAFGFSHLEALLD--DSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMD 146

Query: 152 QLESALQGNETSISHDELVTRSRDQSISD----YGQFVTSGEIQKRSEFFEPFILGLTNG 211
            +E      E   S  +L+    DQS SD    Y + +TSG +Q+ S+FFE FI G    
Sbjct: 147 LIEQV----EKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEG--GR 206

Query: 212 TVDQFCKSAVEPMGEESDHVHIIALSDALGVAIRVLYLDRSSCDSGGLSVNHHDFVPAAS 271
           TV +FC+  VEPM +ESDH+HIIAL+ AL V+I+V Y+DR      G + N H F P  S
Sbjct: 207 TVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGE----GGTTNPHVF-PEGS 266

Query: 272 EVSSDSGAGSEIKIPFITLLYRPGHYDILY 298
           E             P + LLYRPGHYDILY
Sbjct: 267 E-------------PKVYLLYRPGHYDILY 270

BLAST of Lsi07G003890 vs. ExPASy Swiss-Prot
Match: B2RYG6 (Ubiquitin thioesterase OTUB1 OS=Rattus norvegicus OX=10116 GN=Otub1 PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.9e-44
Identity = 116/270 (42.96%), Postives = 155/270 (57.41%), Query Frame = 0

Query: 32  DDDIMLQKSAIFVQEAEKVPFVGDKEPLSSLEAEYKSGSPILLEKIKVLSGQYAAIRRTR 91
           D+ IM Q+  I  + A + P V ++  LS L  EY     I  +KIK L  +Y+ IR+TR
Sbjct: 27  DEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTR 86

Query: 92  GDGNCFFRSFMFSYLEHILESQDRTEVDRIKTNVENCRKTLRSLGYTEFTFEDFFALFLE 151
            DGNCF+R+F FS+LE +L+  D  E+ R K      ++ L S G+TEFT EDF   F++
Sbjct: 87  PDGNCFYRAFGFSHLEALLD--DSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMD 146

Query: 152 QLESALQGNETSISHDELVTRSRDQSISD----YGQFVTSGEIQKRSEFFEPFILGLTNG 211
            +E      E   S  +L+    DQS SD    Y + +TSG +Q+ S+FFE FI G    
Sbjct: 147 LIEQV----EKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEG--GR 206

Query: 212 TVDQFCKSAVEPMGEESDHVHIIALSDALGVAIRVLYLDRSSCDSGGLSVNHHDFVPAAS 271
           TV +FC+  VEPM +ESDH+HIIAL+ AL V+I+V Y+DR      G + N H F P  S
Sbjct: 207 TVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGE----GGTTNPHVF-PEGS 266

Query: 272 EVSSDSGAGSEIKIPFITLLYRPGHYDILY 298
           E             P + LLYRPGHYDILY
Sbjct: 267 E-------------PKVYLLYRPGHYDILY 270

BLAST of Lsi07G003890 vs. ExPASy Swiss-Prot
Match: Q9VL00 (Ubiquitin thioesterase otubain-like OS=Drosophila melanogaster OX=7227 GN=CG4968 PE=2 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 4.8e-43
Identity = 113/273 (41.39%), Postives = 154/273 (56.41%), Query Frame = 0

Query: 32  DDDIMLQKSAIFVQEAEKVPFVGDKEPLSSLEAEYKSGSPILLEKIKVLSGQYAAIRRTR 91
           D+ I+ QK  I  + ++  P V ++ PL+ L AEY SG  I   KI+ LS +Y  IRRTR
Sbjct: 12  DELIIQQKRDIEKEISDTTPLVSEQLPLTCLYAEY-SGDEIFTAKIQDLSKKYKFIRRTR 71

Query: 92  GDGNCFFRSFMFSYLEHILESQDRTEVDRIKTNVENCRKTLRSLGYTEFTFEDFFALFLE 151
            DGNCFFR+F +SYLE+++   + +     K   E  ++ L  LG+  FT EDF   F+E
Sbjct: 72  PDGNCFFRAFAYSYLEYLI--SNTSAYQEFKKLAEESKEKLVQLGFPSFTLEDFHETFME 131

Query: 152 QLESALQGNE--TSISHDELVTRSRDQSISDYG----QFVTSGEIQKRSEFFEPFILGLT 211
            ++     N    S   DEL     +Q  SDY     + +TSG++Q+ ++F++ FI G  
Sbjct: 132 VIQRVSPDNAGGHSTVQDELHKIFNEQGYSDYVVVYLRLITSGKLQEEADFYQNFIEG-- 191

Query: 212 NGTVDQFCKSAVEPMGEESDHVHIIALSDALGVAIRVLYLDRSSCDSGGLSVNHHDFVPA 271
           + T++ F    VEPM +ESDH+HIIAL  ALG  +RV YLDR      G +V  HDF P 
Sbjct: 192 DLTIEAFRHLEVEPMYKESDHIHIIALCTALGAGVRVEYLDRGE----GGTVKAHDF-PE 251

Query: 272 ASEVSSDSGAGSEIKIPFITLLYRPGHYDILYP 299
            SE             P I L+YRPGHYDILYP
Sbjct: 252 GSE-------------PRIYLIYRPGHYDILYP 261

BLAST of Lsi07G003890 vs. ExPASy TrEMBL
Match: A0A1S3BZ50 (Ubiquitin thioesterase OS=Cucumis melo OX=3656 GN=LOC103494633 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 5.7e-156
Identity = 284/303 (93.73%), Postives = 292/303 (96.37%), Query Frame = 0

Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+N+D DGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS
Sbjct: 1   MQNQDNLDTDGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQD+TEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNE+SISHDELV RSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHDELVIRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEIQKRSEFFEPFI+GLTNGTVDQFCK+AVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRVLYLDRSSCDSGGLSVNHHDFVPA +EVSSDS A SEIKIPFITLLYRPGHYDIL
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVSSDS-AASEIKIPFITLLYRPGHYDIL 300

BLAST of Lsi07G003890 vs. ExPASy TrEMBL
Match: A0A0A0KX37 (Ubiquitin thioesterase OS=Cucumis sativus OX=3659 GN=Csa_4G007720 PE=3 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 3.7e-155
Identity = 283/303 (93.40%), Postives = 292/303 (96.37%), Query Frame = 0

Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQEN+DADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS
Sbjct: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQD+TEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNE+SISH+ELV RSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEIQKRSEFFEPFI+GLTNGTVDQFCK+AVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRVLYLDRSSCDSGGLSVNHHDFVPA +EV+S S A SEIKIPFITLLYRPGHYDIL
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGS-AASEIKIPFITLLYRPGHYDIL 300

BLAST of Lsi07G003890 vs. ExPASy TrEMBL
Match: A0A5A7TN16 (Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002440 PE=4 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 5.0e-152
Identity = 280/303 (92.41%), Postives = 288/303 (95.05%), Query Frame = 0

Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQEN+D DGKAES+ISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPF    EPLS
Sbjct: 1   MQNQENLDTDGKAESIISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPF----EPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQD+TEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNE+SISHDELV RSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHDELVIRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEIQKRSEFFEPFI+GLTNGTVDQFCK+AVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRVLYLDRSSCDSGGLSVNHHDFVPA +EVSSDS A SEIKIPFITLLYRPGHYDIL
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVSSDS-AASEIKIPFITLLYRPGHYDIL 298

BLAST of Lsi07G003890 vs. ExPASy TrEMBL
Match: A0A5D3E124 (Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002440 PE=4 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 8.5e-152
Identity = 280/303 (92.41%), Postives = 288/303 (95.05%), Query Frame = 0

Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+N+D DGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPF    EPLS
Sbjct: 1   MQNQDNLDTDGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPF----EPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQD+TEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNE+SISHDELV RSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHDELVIRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEIQKRSEFFEPFI+GLTNGTVDQFCK+AVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRVLYLDRSSCDSGGLSVNHHDFVPA +EVSSDS A SEIKIPFITLLYRPGHYDIL
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVSSDS-AASEIKIPFITLLYRPGHYDIL 298

BLAST of Lsi07G003890 vs. ExPASy TrEMBL
Match: A0A6J1GWQ8 (Ubiquitin thioesterase OS=Cucurbita moschata OX=3662 GN=LOC111457855 PE=3 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 1.9e-151
Identity = 275/303 (90.76%), Postives = 287/303 (94.72%), Query Frame = 0

Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQEN+DAD   ESVISIQTS+YD WGNLGDDDI+ Q+SAIFV+EAEKVPFVGDKEPL+
Sbjct: 1   MQNQENLDADSNVESVISIQTSDYDCWGNLGDDDILQQQSAIFVEEAEKVPFVGDKEPLT 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQY AIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYIAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           I+TNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELV RSRDQSISD
Sbjct: 121 IRTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVIRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEIQKRSEFFEPFI+GLTNGTV+QFCKSAVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVEQFCKSAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRVLYLDRSSCDSGG+SVNHHDFVPA S+VSSDS AGSE KIPFITLLYRPGHYDIL
Sbjct: 241 GVPIRVLYLDRSSCDSGGVSVNHHDFVPAVSDVSSDS-AGSETKIPFITLLYRPGHYDIL 300

BLAST of Lsi07G003890 vs. NCBI nr
Match: XP_038896692.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 [Benincasa hispida] >XP_038896698.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 [Benincasa hispida] >XP_038896706.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 [Benincasa hispida])

HSP 1 Score: 567.4 bits (1461), Expect = 7.4e-158
Identity = 289/303 (95.38%), Postives = 294/303 (97.03%), Query Frame = 0

Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQEN+DADGK ESVISIQTSEYDSWGNLGD+DIMLQKSAIFVQEAEKVPFVGDKEPLS
Sbjct: 1   MQNQENLDADGKVESVISIQTSEYDSWGNLGDEDIMLQKSAIFVQEAEKVPFVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELV RSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVIRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEIQKRSEFFEPFI+GLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDS AGS+IKIPFITLLYRPGHYDIL
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDS-AGSDIKIPFITLLYRPGHYDIL 300

BLAST of Lsi07G003890 vs. NCBI nr
Match: XP_008454143.1 (PREDICTED: ubiquitin thioesterase otubain-like [Cucumis melo] >XP_008454144.1 PREDICTED: ubiquitin thioesterase otubain-like [Cucumis melo])

HSP 1 Score: 560.1 bits (1442), Expect = 1.2e-155
Identity = 284/303 (93.73%), Postives = 292/303 (96.37%), Query Frame = 0

Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+N+D DGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS
Sbjct: 1   MQNQDNLDTDGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQD+TEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNE+SISHDELV RSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHDELVIRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEIQKRSEFFEPFI+GLTNGTVDQFCK+AVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRVLYLDRSSCDSGGLSVNHHDFVPA +EVSSDS A SEIKIPFITLLYRPGHYDIL
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVSSDS-AASEIKIPFITLLYRPGHYDIL 300

BLAST of Lsi07G003890 vs. NCBI nr
Match: XP_004152159.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 [Cucumis sativus] >XP_011653009.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 [Cucumis sativus] >KGN52967.1 hypothetical protein Csa_014673 [Cucumis sativus])

HSP 1 Score: 557.4 bits (1435), Expect = 7.6e-155
Identity = 283/303 (93.40%), Postives = 292/303 (96.37%), Query Frame = 0

Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQEN+DADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS
Sbjct: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQD+TEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNE+SISH+ELV RSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEIQKRSEFFEPFI+GLTNGTVDQFCK+AVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRVLYLDRSSCDSGGLSVNHHDFVPA +EV+S S A SEIKIPFITLLYRPGHYDIL
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGS-AASEIKIPFITLLYRPGHYDIL 300

BLAST of Lsi07G003890 vs. NCBI nr
Match: KAA0044504.1 (ubiquitin thioesterase otubain-like [Cucumis melo var. makuwa])

HSP 1 Score: 547.0 bits (1408), Expect = 1.0e-151
Identity = 280/303 (92.41%), Postives = 288/303 (95.05%), Query Frame = 0

Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQEN+D DGKAES+ISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPF    EPLS
Sbjct: 1   MQNQENLDTDGKAESIISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPF----EPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQD+TEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNE+SISHDELV RSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHDELVIRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEIQKRSEFFEPFI+GLTNGTVDQFCK+AVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRVLYLDRSSCDSGGLSVNHHDFVPA +EVSSDS A SEIKIPFITLLYRPGHYDIL
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVSSDS-AASEIKIPFITLLYRPGHYDIL 298

BLAST of Lsi07G003890 vs. NCBI nr
Match: TYK29632.1 (ubiquitin thioesterase otubain-like [Cucumis melo var. makuwa])

HSP 1 Score: 546.2 bits (1406), Expect = 1.8e-151
Identity = 280/303 (92.41%), Postives = 288/303 (95.05%), Query Frame = 0

Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+N+D DGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPF    EPLS
Sbjct: 1   MQNQDNLDTDGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPF----EPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQD+TEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNE+SISHDELV RSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHDELVIRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEIQKRSEFFEPFI+GLTNGTVDQFCK+AVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRVLYLDRSSCDSGGLSVNHHDFVPA +EVSSDS A SEIKIPFITLLYRPGHYDIL
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVSSDS-AASEIKIPFITLLYRPGHYDIL 298

BLAST of Lsi07G003890 vs. TAIR 10
Match: AT1G28120.1 (CONTAINS InterPro DOMAIN/s: Ovarian tumour, otubain (InterPro:IPR003323), Ubiquitin thioesterase Otubain (InterPro:IPR016615), Peptidase C65, otubain (InterPro:IPR019400); Has 413 Blast hits to 411 proteins in 139 species: Archae - 0; Bacteria - 0; Metazoa - 184; Fungi - 83; Plants - 88; Viruses - 0; Other Eukaryotes - 58 (source: NCBI BLink). )

HSP 1 Score: 412.9 bits (1060), Expect = 2.2e-115
Identity = 211/303 (69.64%), Postives = 241/303 (79.54%), Query Frame = 0

Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ ++  D +AE   SI   + + WGN    +    + +   +EA KVP+VGDKEPLS
Sbjct: 1   MQNQIDMVKD-EAEVAASISAIKGEEWGNCSSVE---DQPSFQEEEAAKVPYVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SL AEY+SGSPILLEKIK+L  QY  IRRTRGDGNCFFRSFMFSYLEHILESQDR EVDR
Sbjct: 61  SLAAEYQSGSPILLEKIKILDSQYIGIRRTRGDGNCFFRSFMFSYLEHILESQDRAEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IK NVE CRKTL++LGYT+FTFEDFFALFLEQL+  LQG E SIS+DELV RSRDQS+SD
Sbjct: 121 IKVNVEKCRKTLQNLGYTDFTFEDFFALFLEQLDDILQGTEESISYDELVNRSRDQSVSD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVT+G+I+ R++FFEPFI GL+N TVDQFCKS+VEPMGEESDH+HI ALSDAL
Sbjct: 181 YIVMFFRFVTAGDIRTRADFFEPFITGLSNATVDQFCKSSVEPMGEESDHIHITALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GVAIRV+YLDRSSCDSGG++VNHHDFVP       D     E   PFITLLYRPGHYDIL
Sbjct: 241 GVAIRVVYLDRSSCDSGGVTVNHHDFVPVGITNEKD----EEASAPFITLLYRPGHYDIL 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LG983.0e-11469.64OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 OS=Arabidopsis thalian... [more]
Q96FW11.9e-4442.96Ubiquitin thioesterase OTUB1 OS=Homo sapiens OX=9606 GN=OTUB1 PE=1 SV=2[more]
Q7TQI31.9e-4442.96Ubiquitin thioesterase OTUB1 OS=Mus musculus OX=10090 GN=Otub1 PE=1 SV=2[more]
B2RYG61.9e-4442.96Ubiquitin thioesterase OTUB1 OS=Rattus norvegicus OX=10116 GN=Otub1 PE=1 SV=1[more]
Q9VL004.8e-4341.39Ubiquitin thioesterase otubain-like OS=Drosophila melanogaster OX=7227 GN=CG4968... [more]
Match NameE-valueIdentityDescription
A0A1S3BZ505.7e-15693.73Ubiquitin thioesterase OS=Cucumis melo OX=3656 GN=LOC103494633 PE=3 SV=1[more]
A0A0A0KX373.7e-15593.40Ubiquitin thioesterase OS=Cucumis sativus OX=3659 GN=Csa_4G007720 PE=3 SV=1[more]
A0A5A7TN165.0e-15292.41Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5D3E1248.5e-15292.41Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1GWQ81.9e-15190.76Ubiquitin thioesterase OS=Cucurbita moschata OX=3662 GN=LOC111457855 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038896692.17.4e-15895.38OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 [Benincasa hispida] >X... [more]
XP_008454143.11.2e-15593.73PREDICTED: ubiquitin thioesterase otubain-like [Cucumis melo] >XP_008454144.1 PR... [more]
XP_004152159.17.6e-15593.40OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 [Cucumis sativus] >XP_... [more]
KAA0044504.11.0e-15192.41ubiquitin thioesterase otubain-like [Cucumis melo var. makuwa][more]
TYK29632.11.8e-15192.41ubiquitin thioesterase otubain-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G28120.12.2e-11569.64CONTAINS InterPro DOMAIN/s: Ovarian tumour, otubain (InterPro:IPR003323), Ubiqui... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042468Peptidase C65, otubain, subdomain 1GENE3D3.30.200.60Peptidase C65 Otubain, subdomain 1coord: 51..297
e-value: 6.2E-74
score: 250.4
IPR016615Ubiquitin thioesterase OtubainPIRSFPIRSF013503OTUBcoord: 21..299
e-value: 3.2E-84
score: 280.1
IPR042467Peptidase C65, otubain, subdomain 2GENE3D1.20.1300.20Peptidase C65 Otubain, subdomain 2coord: 91..238
e-value: 6.2E-74
score: 250.4
IPR019400Peptidase C65, otubainPFAMPF10275Peptidase_C65coord: 46..298
e-value: 1.0E-80
score: 270.7
IPR019400Peptidase C65, otubainPANTHERPTHR12931UBIQUITIN THIOLESTERASE PROTEIN OTUBcoord: 46..299
NoneNo IPR availablePIRSRPIRSR013503-2PIRSR013503-2coord: 64..294
e-value: 7.0E-70
score: 232.8
NoneNo IPR availablePANTHERPTHR12931:SF30UBIQUITIN THIOESTERASEcoord: 46..299
IPR003323OTU domainPROSITEPS50802OTUcoord: 85..299
score: 12.061941
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 52..298

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G003890.1Lsi07G003890.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071108 protein K48-linked deubiquitination
biological_process GO:0016579 protein deubiquitination
cellular_component GO:0005634 nucleus
molecular_function GO:0061815 deubiquitinase, acting on linear ubiquitin
molecular_function GO:1990380 Lys48-specific deubiquitinase activity
molecular_function GO:0019784 NEDD8-specific protease activity
molecular_function GO:0004843 thiol-dependent deubiquitinase
molecular_function GO:0043130 ubiquitin binding
molecular_function GO:0016787 hydrolase activity