Lsi07G003810 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi07G003810 vs. ExPASy Swiss-Prot
Match: Q8GWB2 (Protein NEOXANTHIN-DEFICIENT 1 OS=Arabidopsis thaliana OX=3702 GN=NDX1 PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 4.9e-68
Identity = 153/329 (46.50%), Postives = 197/329 (59.88%), Query Frame = 0

Query: 1   MEVGEKNCSSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLAN 60
           M+V EK  SSGY KPPWIF+G ALYQ+HLVKA TARA IPKE RLVEAFGYTLGGFFLA+
Sbjct: 1   MDVEEKRVSSGYAKPPWIFKGSALYQIHLVKAATARAFIPKEFRLVEAFGYTLGGFFLAS 60

Query: 61  YEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKRI 120
           Y+DSPAG FDELVVI+GIVWN PTSCAWAA+VLVNS +AC HGRKEVGLPSQVARF+K I
Sbjct: 61  YDDSPAGVFDELVVIAGIVWNPPTSCAWAARVLVNSDEACHHGRKEVGLPSQVARFSKNI 120

Query: 121 EAVPMRQSER--GLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFAS 180
            AVP ++ +R  G L++    + +   ++   V+V+EV    S D   IC     ++ + 
Sbjct: 121 TAVPKQKRDRAFGFLDTFGLGTTLSHPENLMEVKVSEVDSAASTD---ICN---IQIRSD 180

Query: 181 VPVIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDI 240
              + N  + G    +  P S S NT+                                 
Sbjct: 181 ETKVGN--WMGPAIKMALP-SFSGNTI--------------------------------Y 240

Query: 241 QNILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDHHAGEDAEHEQS 300
            + LL YS          C     VR V+PA VS  L    ++  +++H + E  E+E+ 
Sbjct: 241 NSNLLKYS----------CHLHCRVRPVRPAVVSGALEDETEKFTEQNHTSQESLENERQ 278

Query: 301 LCTSVLLSKPILALEFSCMEMQVQAPAVV 328
           L  +V+LSKPI+AL+F C+ MQV+AP V+
Sbjct: 301 LSKAVMLSKPIIALQFKCLTMQVEAPVVI 278

BLAST of Lsi07G003810 vs. ExPASy Swiss-Prot
Match: K4DEY3 (Protein NEOXANTHIN-DEFICIENT 1 OS=Solanum lycopersicum OX=4081 GN=NXD1 PE=4 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 5.4e-59
Identity = 149/344 (43.31%), Postives = 195/344 (56.69%), Query Frame = 0

Query: 1   MEVGEKNCSS-GYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLA 60
           MEV + NC+S GYGKPPWIF+G ALYQLHLVKA+ ARA IPKE +LVEAFGYTLGGFFLA
Sbjct: 1   MEVKDTNCTSLGYGKPPWIFKGSALYQLHLVKAENARAFIPKECKLVEAFGYTLGGFFLA 60

Query: 61  NYEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKR 120
           +Y+DSPAG FDELVVI+G+VWN PTSCAWAA+VLV S +AC HGRK VGLPSQVARF+K+
Sbjct: 61  SYDDSPAGIFDELVVIAGLVWNPPTSCAWAARVLVGSDEACLHGRKVVGLPSQVARFSKK 120

Query: 121 IEAVPMRQSERG----LLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSEL 180
           I A+P +   +         LR +SN    K+   V+VTE+K  T+   + IC       
Sbjct: 121 ITALPQKPESKSSSFLRRIGLRTSSN---YKNHMDVEVTEIKKQTA---MSIC------- 180

Query: 181 FASVPVIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVI 240
                            N+   A++S                   + GW         + 
Sbjct: 181 -----------------NINVNATASQQ----------------DSKGW---------MG 240

Query: 241 VDIQNILLNYSNIPAKFNAGL----CFCSLMVRAVKPAAVSVKLPTSIDRAQDRDHHAGE 300
             I+  L N+S    K+N+ L    C     VRAV+PA VS    +  D+    +  +  
Sbjct: 241 PLIKMSLPNFSG-RTKYNSDLLKYSCQIECRVRAVQPAKVSGPSESDADKENSSEDQSSN 288

Query: 301 -------DAEHEQSLCTSVLLSKPILALEFSCMEMQVQAPAVVS 329
                      +++   SV+LSKPILALEF+ ++M+V+AP  V+
Sbjct: 301 VESVSRVPRGTKRNFSISVMLSKPILALEFNHLKMRVEAPTTVT 288

BLAST of Lsi07G003810 vs. ExPASy Swiss-Prot
Match: Q0IWM5 (Protein NEOXANTHIN-DEFICIENT 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NDX1 PE=3 SV=2)

HSP 1 Score: 210.3 bits (534), Expect = 3.4e-53
Identity = 143/338 (42.31%), Postives = 180/338 (53.25%), Query Frame = 0

Query: 5   EKNCSSGYGK-PPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLANYED 64
           E   ++GYG+ PPW+FRGRALYQLHLVKA TARA +P+ELRLVEAFGYTLGG FLA Y+D
Sbjct: 9   EAAAAAGYGRGPPWVFRGRALYQLHLVKAATARAFVPRELRLVEAFGYTLGGMFLARYDD 68

Query: 65  SPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKRIEAV 124
           SPAG FDELVVI+GIVWN PTSCAWAA+VLVNS +AC HGRKEVGLPS VA F+ + EA 
Sbjct: 69  SPAGKFDELVVIAGIVWNPPTSCAWAARVLVNSAEACRHGRKEVGLPSHVATFS-QTEAD 128

Query: 125 PMRQ----SERGLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFASV 184
            +R          L+ L   S + +Q +   ++++E KG             C+    ++
Sbjct: 129 ALRNKPLVKSNSFLSLLGMRSTVSNQGNDREIEISETKG------------SCTRHLCNI 188

Query: 185 PVIANQPYPGSDFNVYF-PASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDI 244
            V    P  GS  + +  PA   S  L ++   +E                         
Sbjct: 189 SV----PLTGSHKHKWMGPAIRMS--LPSFSGQIED------------------------ 248

Query: 245 QNILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQD-RDHHAGEDAEHE- 304
              LL YS          C     VR V+PA +     T      D +    G +   E 
Sbjct: 249 HPDLLKYS----------CQVECRVRPVRPAKIWRPRITEPQECPDGKISSKGSEVLAEP 293

Query: 305 --QSLCTSVLLSKPILALEFSCMEMQVQAPAVVSQYFK 333
             Q     VLLSKPILALEF+ +EM V AP +V  + K
Sbjct: 309 DAQKHTVMVLLSKPILALEFNSLEMHVDAPKIVIPHSK 293

BLAST of Lsi07G003810 vs. ExPASy TrEMBL
Match: A0A5D3E1J6 (Protein NEOXANTHIN-DEFICIENT 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002500 PE=4 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 3.2e-107
Identity = 223/344 (64.83%), Postives = 243/344 (70.64%), Query Frame = 0

Query: 1   MEVGEKNC-SSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLA 60
           ME+G++ C SSGYGKPPW FRGRALYQLHLVKA TARACIPKELRLVEAFGYTLGGFFLA
Sbjct: 1   MEIGDQKCSSSGYGKPPWKFRGRALYQLHLVKAGTARACIPKELRLVEAFGYTLGGFFLA 60

Query: 61  NYEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKR 120
           NY+DSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSV+ACDHGRKEVGLPS VARFTKR
Sbjct: 61  NYDDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVEACDHGRKEVGLPSHVARFTKR 120

Query: 121 IEAVPMRQSERGLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFASV 180
           IEAVP RQSERGLL+ LR NSN ++QK+QEHVQVTEVKGPTSID   +C    S    SV
Sbjct: 121 IEAVPKRQSERGLLSFLRENSNFHNQKNQEHVQVTEVKGPTSID---VCNINLS---FSV 180

Query: 181 PVIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDIQ 240
           P      + G    +  P+ S                                    +  
Sbjct: 181 PF---SKWMGPAIKMSLPSYSGH---------------------------------TEYS 240

Query: 241 NILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDHH-----AGEDAE 300
             LL YS          C     VRAVKPA VSV+LP +++RA+D DHH     +GED E
Sbjct: 241 PELLKYS----------CQIQCRVRAVKPATVSVELP-ALNRAEDGDHHSHITRSGEDGE 291

Query: 301 HEQSLCTSVLLSKPILALEFSCMEMQVQAPAVVSQYFKHSLRTP 339
           HEQSLCTSVLLSKPILALEFSCMEMQVQAP VVSQYF HSLRTP
Sbjct: 301 HEQSLCTSVLLSKPILALEFSCMEMQVQAPTVVSQYFNHSLRTP 291

BLAST of Lsi07G003810 vs. ExPASy TrEMBL
Match: A0A1S3BZ40 (protein NEOXANTHIN-DEFICIENT 1 OS=Cucumis melo OX=3656 GN=LOC103494625 PE=4 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 3.2e-107
Identity = 223/344 (64.83%), Postives = 243/344 (70.64%), Query Frame = 0

Query: 1   MEVGEKNC-SSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLA 60
           ME+G++ C SSGYGKPPW FRGRALYQLHLVKA TARACIPKELRLVEAFGYTLGGFFLA
Sbjct: 1   MEIGDQKCSSSGYGKPPWKFRGRALYQLHLVKAGTARACIPKELRLVEAFGYTLGGFFLA 60

Query: 61  NYEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKR 120
           NY+DSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSV+ACDHGRKEVGLPS VARFTKR
Sbjct: 61  NYDDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVEACDHGRKEVGLPSHVARFTKR 120

Query: 121 IEAVPMRQSERGLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFASV 180
           IEAVP RQSERGLL+ LR NSN ++QK+QEHVQVTEVKGPTSID   +C    S    SV
Sbjct: 121 IEAVPKRQSERGLLSFLRENSNFHNQKNQEHVQVTEVKGPTSID---VCNINLS---FSV 180

Query: 181 PVIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDIQ 240
           P      + G    +  P+ S                                    +  
Sbjct: 181 PF---SKWMGPAIKMSLPSYSGH---------------------------------TEYS 240

Query: 241 NILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDHH-----AGEDAE 300
             LL YS          C     VRAVKPA VSV+LP +++RA+D DHH     +GED E
Sbjct: 241 PELLKYS----------CQIQCRVRAVKPATVSVELP-ALNRAEDGDHHSHITRSGEDGE 291

Query: 301 HEQSLCTSVLLSKPILALEFSCMEMQVQAPAVVSQYFKHSLRTP 339
           HEQSLCTSVLLSKPILALEFSCMEMQVQAP VVSQYF HSLRTP
Sbjct: 301 HEQSLCTSVLLSKPILALEFSCMEMQVQAPTVVSQYFNHSLRTP 291

BLAST of Lsi07G003810 vs. ExPASy TrEMBL
Match: A0A5A7TTG0 (Protein NEOXANTHIN-DEFICIENT 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002490 PE=4 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.6e-106
Identity = 222/344 (64.53%), Postives = 242/344 (70.35%), Query Frame = 0

Query: 1   MEVGEKNC-SSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLA 60
           ME+G++ C SSGYGKPPW FRGRALYQLHLVKA TARACIPKELRLVEAFGYTLGGFFLA
Sbjct: 1   MEIGDQKCSSSGYGKPPWKFRGRALYQLHLVKAGTARACIPKELRLVEAFGYTLGGFFLA 60

Query: 61  NYEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKR 120
           NY+DSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSV+ACDHGRKEVGLPS VARFTKR
Sbjct: 61  NYDDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVEACDHGRKEVGLPSHVARFTKR 120

Query: 121 IEAVPMRQSERGLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFASV 180
           IEAVP RQSERGLL+  R NSN ++QK+QEHVQVTEVKGPTSID   +C    S    SV
Sbjct: 121 IEAVPKRQSERGLLSFSRENSNFHNQKNQEHVQVTEVKGPTSID---VCNINLS---FSV 180

Query: 181 PVIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDIQ 240
           P      + G    +  P+ S                                    +  
Sbjct: 181 PF---SKWMGPAIKMSLPSYSGH---------------------------------TEYS 240

Query: 241 NILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDHH-----AGEDAE 300
             LL YS          C     VRAVKPA VSV+LP +++RA+D DHH     +GED E
Sbjct: 241 PELLKYS----------CQIQCRVRAVKPATVSVELP-ALNRAEDGDHHSHITRSGEDGE 291

Query: 301 HEQSLCTSVLLSKPILALEFSCMEMQVQAPAVVSQYFKHSLRTP 339
           HEQSLCTSVLLSKPILALEFSCMEMQVQAP VVSQYF HSLRTP
Sbjct: 301 HEQSLCTSVLLSKPILALEFSCMEMQVQAPTVVSQYFNHSLRTP 291

BLAST of Lsi07G003810 vs. ExPASy TrEMBL
Match: A0A6J1CWZ7 (protein NEOXANTHIN-DEFICIENT 1 OS=Momordica charantia OX=3673 GN=LOC111015179 PE=4 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 7.2e-99
Identity = 209/342 (61.11%), Postives = 227/342 (66.37%), Query Frame = 0

Query: 1   MEVGEKNCSSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLAN 60
           MEVGE+NCS GYG+PPW FRGRALYQLHLVK K ARACIPKELRLVEAFGYTLGGFFLA+
Sbjct: 56  MEVGERNCSPGYGRPPWTFRGRALYQLHLVKGKIARACIPKELRLVEAFGYTLGGFFLAS 115

Query: 61  YEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKRI 120
           Y+DSPAGTFDELVVI+GIVWNRPTSCAWAAKVLVNSV ACDHGRKE+GLPSQVARFTKRI
Sbjct: 116 YDDSPAGTFDELVVIAGIVWNRPTSCAWAAKVLVNSVQACDHGRKEIGLPSQVARFTKRI 175

Query: 121 EAVPMRQSERGLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFASVP 180
           EAVP  +SE GLLNSL G  N+Y+QK+QEHVQVTEVKGPTS     IC    S    SVP
Sbjct: 176 EAVPKHRSESGLLNSLGGKINVYNQKNQEHVQVTEVKGPTSTS---ICNINLS---TSVP 235

Query: 181 VIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDIQN 240
           +     + G    +  P+ S     T                                  
Sbjct: 236 L---NKWMGPAIKMSLPSYSGHTEYTPE-------------------------------- 295

Query: 241 ILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDH----HAGEDAEHE 300
            L  YS          C     VRAVKP  VSV+ P     AQ+  H      GE AE E
Sbjct: 296 -LFKYS----------CQIRCRVRAVKPMKVSVEFP-----AQNEHHSCTRRGGEGAEEE 340

Query: 301 QSLCTSVLLSKPILALEFSCMEMQVQAPAVVSQYFKHSLRTP 339
           QSL TSVLLSKPILALEFSCMEM+V+AP VVSQYF HSLRTP
Sbjct: 356 QSLSTSVLLSKPILALEFSCMEMKVEAPTVVSQYFNHSLRTP 340

BLAST of Lsi07G003810 vs. ExPASy TrEMBL
Match: A0A6J1IS89 (protein NEOXANTHIN-DEFICIENT 1 OS=Cucurbita maxima OX=3661 GN=LOC111480077 PE=4 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 5.2e-97
Identity = 208/338 (61.54%), Postives = 229/338 (67.75%), Query Frame = 0

Query: 1   MEVGEKNCSSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLAN 60
           ME GEK  S+GYG+PPW FRGRALYQLHLVKAKTAR CIPKELRLVE FGYTLGGFFLAN
Sbjct: 1   MENGEKKRSTGYGRPPWTFRGRALYQLHLVKAKTARKCIPKELRLVEVFGYTLGGFFLAN 60

Query: 61  YEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKRI 120
           Y+DSPAG+FDELVVI+GIVWNRPTSCAWAAKVLVNS +ACDHGRKEVGLPSQVARFTKRI
Sbjct: 61  YDDSPAGSFDELVVIAGIVWNRPTSCAWAAKVLVNSDEACDHGRKEVGLPSQVARFTKRI 120

Query: 121 EAVPMRQSERGLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFASVP 180
           EAVP  +SERGLLNS RG+S+  +QK+QEHVQVTEVK PTSID   +C    S    SVP
Sbjct: 121 EAVPKHRSERGLLNSFRGSSDFCNQKNQEHVQVTEVKNPTSID---VCNINLS---ISVP 180

Query: 181 VIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDIQN 240
           +     + G    +  P+ S     T                                  
Sbjct: 181 L---SKWMGPAIRMSLPSYSGHTENTPE-------------------------------- 240

Query: 241 ILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDHHAGEDAEHEQSLC 300
            LL YS          C     VRAVKPAAV      +I+RA + + H     EHEQSL 
Sbjct: 241 -LLKYS----------CQIQCRVRAVKPAAV------TIERAGEDEQH-----EHEQSLS 275

Query: 301 TSVLLSKPILALEFSCMEMQVQAPAVVSQYFKHSLRTP 339
           T+VLLSKPILALEFSCMEMQVQAP VVSQYFKHSLRTP
Sbjct: 301 TTVLLSKPILALEFSCMEMQVQAPTVVSQYFKHSLRTP 275

BLAST of Lsi07G003810 vs. NCBI nr
Match: XP_038898326.1 (protein NEOXANTHIN-DEFICIENT 1 [Benincasa hispida])

HSP 1 Score: 419.5 bits (1077), Expect = 2.8e-113
Identity = 230/343 (67.06%), Postives = 245/343 (71.43%), Query Frame = 0

Query: 1   MEVGEKNCSSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLAN 60
           ME GEKNCSSGYGKPPW F GRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLAN
Sbjct: 1   METGEKNCSSGYGKPPWTFTGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLAN 60

Query: 61  YEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKRI 120
           Y+DSPAG+FDELVVISGIVWNRPTSCAWAAKVLVNS +ACDHGRKEVGLPSQ ARFTKRI
Sbjct: 61  YDDSPAGSFDELVVISGIVWNRPTSCAWAAKVLVNSDEACDHGRKEVGLPSQAARFTKRI 120

Query: 121 EAVPMRQSERGLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFASVP 180
           EAVP RQSERGLLNSLR NSN ++QK+QEH+QVTE+KGPTSID   +C    S    SVP
Sbjct: 121 EAVPKRQSERGLLNSLRENSNFHNQKNQEHIQVTEMKGPTSID---VCNINLS---ISVP 180

Query: 181 VIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDIQN 240
                 + G    +  P+ S  +  T                                  
Sbjct: 181 F---TKWMGPVIKMSLPSYSGHSEYTPE-------------------------------- 240

Query: 241 ILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDHH-----AGEDAEH 300
            LL YS          C     VRAVKPA VSV+LP S DRAQD DHH     AGEDAEH
Sbjct: 241 -LLKYS----------CQIRCRVRAVKPAVVSVELPASTDRAQDADHHSHNTRAGEDAEH 291

Query: 301 EQSLCTSVLLSKPILALEFSCMEMQVQAPAVVSQYFKHSLRTP 339
           EQSLCTSVLLSKPILALEFSCMEM+VQAP VVSQYFKHSLRTP
Sbjct: 301 EQSLCTSVLLSKPILALEFSCMEMEVQAPTVVSQYFKHSLRTP 291

BLAST of Lsi07G003810 vs. NCBI nr
Match: XP_008454132.1 (PREDICTED: protein NEOXANTHIN-DEFICIENT 1 [Cucumis melo] >TYK29638.1 protein NEOXANTHIN-DEFICIENT 1 [Cucumis melo var. makuwa])

HSP 1 Score: 398.3 bits (1022), Expect = 6.7e-107
Identity = 223/344 (64.83%), Postives = 243/344 (70.64%), Query Frame = 0

Query: 1   MEVGEKNC-SSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLA 60
           ME+G++ C SSGYGKPPW FRGRALYQLHLVKA TARACIPKELRLVEAFGYTLGGFFLA
Sbjct: 1   MEIGDQKCSSSGYGKPPWKFRGRALYQLHLVKAGTARACIPKELRLVEAFGYTLGGFFLA 60

Query: 61  NYEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKR 120
           NY+DSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSV+ACDHGRKEVGLPS VARFTKR
Sbjct: 61  NYDDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVEACDHGRKEVGLPSHVARFTKR 120

Query: 121 IEAVPMRQSERGLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFASV 180
           IEAVP RQSERGLL+ LR NSN ++QK+QEHVQVTEVKGPTSID   +C    S    SV
Sbjct: 121 IEAVPKRQSERGLLSFLRENSNFHNQKNQEHVQVTEVKGPTSID---VCNINLS---FSV 180

Query: 181 PVIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDIQ 240
           P      + G    +  P+ S                                    +  
Sbjct: 181 PF---SKWMGPAIKMSLPSYSGH---------------------------------TEYS 240

Query: 241 NILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDHH-----AGEDAE 300
             LL YS          C     VRAVKPA VSV+LP +++RA+D DHH     +GED E
Sbjct: 241 PELLKYS----------CQIQCRVRAVKPATVSVELP-ALNRAEDGDHHSHITRSGEDGE 291

Query: 301 HEQSLCTSVLLSKPILALEFSCMEMQVQAPAVVSQYFKHSLRTP 339
           HEQSLCTSVLLSKPILALEFSCMEMQVQAP VVSQYF HSLRTP
Sbjct: 301 HEQSLCTSVLLSKPILALEFSCMEMQVQAPTVVSQYFNHSLRTP 291

BLAST of Lsi07G003810 vs. NCBI nr
Match: KAA0044509.1 (protein NEOXANTHIN-DEFICIENT 1 [Cucumis melo var. makuwa])

HSP 1 Score: 396.0 bits (1016), Expect = 3.3e-106
Identity = 222/344 (64.53%), Postives = 242/344 (70.35%), Query Frame = 0

Query: 1   MEVGEKNC-SSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLA 60
           ME+G++ C SSGYGKPPW FRGRALYQLHLVKA TARACIPKELRLVEAFGYTLGGFFLA
Sbjct: 1   MEIGDQKCSSSGYGKPPWKFRGRALYQLHLVKAGTARACIPKELRLVEAFGYTLGGFFLA 60

Query: 61  NYEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKR 120
           NY+DSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSV+ACDHGRKEVGLPS VARFTKR
Sbjct: 61  NYDDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVEACDHGRKEVGLPSHVARFTKR 120

Query: 121 IEAVPMRQSERGLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFASV 180
           IEAVP RQSERGLL+  R NSN ++QK+QEHVQVTEVKGPTSID   +C    S    SV
Sbjct: 121 IEAVPKRQSERGLLSFSRENSNFHNQKNQEHVQVTEVKGPTSID---VCNINLS---FSV 180

Query: 181 PVIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDIQ 240
           P      + G    +  P+ S                                    +  
Sbjct: 181 PF---SKWMGPAIKMSLPSYSGH---------------------------------TEYS 240

Query: 241 NILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDHH-----AGEDAE 300
             LL YS          C     VRAVKPA VSV+LP +++RA+D DHH     +GED E
Sbjct: 241 PELLKYS----------CQIQCRVRAVKPATVSVELP-ALNRAEDGDHHSHITRSGEDGE 291

Query: 301 HEQSLCTSVLLSKPILALEFSCMEMQVQAPAVVSQYFKHSLRTP 339
           HEQSLCTSVLLSKPILALEFSCMEMQVQAP VVSQYF HSLRTP
Sbjct: 301 HEQSLCTSVLLSKPILALEFSCMEMQVQAPTVVSQYFNHSLRTP 291

BLAST of Lsi07G003810 vs. NCBI nr
Match: XP_004152157.1 (protein NEOXANTHIN-DEFICIENT 1 isoform X2 [Cucumis sativus] >KAE8649081.1 hypothetical protein Csa_015269 [Cucumis sativus])

HSP 1 Score: 390.6 bits (1002), Expect = 1.4e-104
Identity = 218/344 (63.37%), Postives = 240/344 (69.77%), Query Frame = 0

Query: 1   MEVGEKNC-SSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLA 60
           ME+G++ C SSGYGKPPW+FRGRALYQLHLVKA TARACIPKELRLVEAFGYTLGGFFLA
Sbjct: 1   MEIGDQKCSSSGYGKPPWMFRGRALYQLHLVKATTARACIPKELRLVEAFGYTLGGFFLA 60

Query: 61  NYEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKR 120
           NY+DSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNS +ACDHGRKEVGLPSQVARFTKR
Sbjct: 61  NYDDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSAEACDHGRKEVGLPSQVARFTKR 120

Query: 121 IEAVPMRQSERGLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFASV 180
           IEAVP  QSE+GLL+ LRGNSN ++QK+QEHVQV EVKGPTS+D   +C    S    SV
Sbjct: 121 IEAVPKHQSEKGLLSFLRGNSNFHNQKNQEHVQVAEVKGPTSMD---VCNINLS---FSV 180

Query: 181 PVIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDIQ 240
           P      + G    +  P+ S     T                                 
Sbjct: 181 PF---SKWMGPAIKMSLPSYSGHTEYTPE------------------------------- 240

Query: 241 NILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDHHA-----GEDAE 300
             LL YS          C     VRAVKPA VS+    +++RA+D DHH+     GE  E
Sbjct: 241 --LLKYS----------CQIRCRVRAVKPATVSI---PALNRAEDGDHHSHITRTGEYGE 289

Query: 301 HEQSLCTSVLLSKPILALEFSCMEMQVQAPAVVSQYFKHSLRTP 339
           HEQSLCTSVLLSKPILALEFSCMEMQVQAP VVSQYFKHSLRTP
Sbjct: 301 HEQSLCTSVLLSKPILALEFSCMEMQVQAPTVVSQYFKHSLRTP 289

BLAST of Lsi07G003810 vs. NCBI nr
Match: XP_022145818.1 (protein NEOXANTHIN-DEFICIENT 1 [Momordica charantia])

HSP 1 Score: 370.5 bits (950), Expect = 1.5e-98
Identity = 209/342 (61.11%), Postives = 227/342 (66.37%), Query Frame = 0

Query: 1   MEVGEKNCSSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLAN 60
           MEVGE+NCS GYG+PPW FRGRALYQLHLVK K ARACIPKELRLVEAFGYTLGGFFLA+
Sbjct: 56  MEVGERNCSPGYGRPPWTFRGRALYQLHLVKGKIARACIPKELRLVEAFGYTLGGFFLAS 115

Query: 61  YEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKRI 120
           Y+DSPAGTFDELVVI+GIVWNRPTSCAWAAKVLVNSV ACDHGRKE+GLPSQVARFTKRI
Sbjct: 116 YDDSPAGTFDELVVIAGIVWNRPTSCAWAAKVLVNSVQACDHGRKEIGLPSQVARFTKRI 175

Query: 121 EAVPMRQSERGLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFASVP 180
           EAVP  +SE GLLNSL G  N+Y+QK+QEHVQVTEVKGPTS     IC    S    SVP
Sbjct: 176 EAVPKHRSESGLLNSLGGKINVYNQKNQEHVQVTEVKGPTSTS---ICNINLS---TSVP 235

Query: 181 VIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDIQN 240
           +     + G    +  P+ S     T                                  
Sbjct: 236 L---NKWMGPAIKMSLPSYSGHTEYTPE-------------------------------- 295

Query: 241 ILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDH----HAGEDAEHE 300
            L  YS          C     VRAVKP  VSV+ P     AQ+  H      GE AE E
Sbjct: 296 -LFKYS----------CQIRCRVRAVKPMKVSVEFP-----AQNEHHSCTRRGGEGAEEE 340

Query: 301 QSLCTSVLLSKPILALEFSCMEMQVQAPAVVSQYFKHSLRTP 339
           QSL TSVLLSKPILALEFSCMEM+V+AP VVSQYF HSLRTP
Sbjct: 356 QSLSTSVLLSKPILALEFSCMEMKVEAPTVVSQYFNHSLRTP 340

BLAST of Lsi07G003810 vs. TAIR 10
Match: AT1G28100.1 (unknown protein; Has 64 Blast hits to 64 proteins in 27 species: Archae - 0; Bacteria - 14; Metazoa - 0; Fungi - 6; Plants - 42; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 259.6 bits (662), Expect = 3.5e-69
Identity = 153/329 (46.50%), Postives = 197/329 (59.88%), Query Frame = 0

Query: 1   MEVGEKNCSSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLAN 60
           M+V EK  SSGY KPPWIF+G ALYQ+HLVKA TARA IPKE RLVEAFGYTLGGFFLA+
Sbjct: 1   MDVEEKRVSSGYAKPPWIFKGSALYQIHLVKAATARAFIPKEFRLVEAFGYTLGGFFLAS 60

Query: 61  YEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKRI 120
           Y+DSPAG FDELVVI+GIVWN PTSCAWAA+VLVNS +AC HGRKEVGLPSQVARF+K I
Sbjct: 61  YDDSPAGVFDELVVIAGIVWNPPTSCAWAARVLVNSDEACHHGRKEVGLPSQVARFSKNI 120

Query: 121 EAVPMRQSER--GLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFAS 180
            AVP ++ +R  G L++    + +   ++   V+V+EV    S D   IC     ++ + 
Sbjct: 121 TAVPKQKRDRAFGFLDTFGLGTTLSHPENLMEVKVSEVDSAASTD---ICN---IQIRSD 180

Query: 181 VPVIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDI 240
              + N  + G    +  P S S NT+                                 
Sbjct: 181 ETKVGN--WMGPAIKMALP-SFSGNTI--------------------------------Y 240

Query: 241 QNILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDHHAGEDAEHEQS 300
            + LL YS          C     VR V+PA VS  L    ++  +++H + E  E+E+ 
Sbjct: 241 NSNLLKYS----------CHLHCRVRPVRPAVVSGALEDETEKFTEQNHTSQESLENERQ 278

Query: 301 LCTSVLLSKPILALEFSCMEMQVQAPAVV 328
           L  +V+LSKPI+AL+F C+ MQV+AP V+
Sbjct: 301 LSKAVMLSKPIIALQFKCLTMQVEAPVVI 278

BLAST of Lsi07G003810 vs. TAIR 10
Match: AT1G28100.3 (unknown protein; Has 64 Blast hits to 64 proteins in 27 species: Archae - 0; Bacteria - 14; Metazoa - 0; Fungi - 6; Plants - 42; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 259.6 bits (662), Expect = 3.5e-69
Identity = 153/329 (46.50%), Postives = 197/329 (59.88%), Query Frame = 0

Query: 1   MEVGEKNCSSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLAN 60
           M+V EK  SSGY KPPWIF+G ALYQ+HLVKA TARA IPKE RLVEAFGYTLGGFFLA+
Sbjct: 1   MDVEEKRVSSGYAKPPWIFKGSALYQIHLVKAATARAFIPKEFRLVEAFGYTLGGFFLAS 60

Query: 61  YEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKRI 120
           Y+DSPAG FDELVVI+GIVWN PTSCAWAA+VLVNS +AC HGRKEVGLPSQVARF+K I
Sbjct: 61  YDDSPAGVFDELVVIAGIVWNPPTSCAWAARVLVNSDEACHHGRKEVGLPSQVARFSKNI 120

Query: 121 EAVPMRQSER--GLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFAS 180
            AVP ++ +R  G L++    + +   ++   V+V+EV    S D   IC     ++ + 
Sbjct: 121 TAVPKQKRDRAFGFLDTFGLGTTLSHPENLMEVKVSEVDSAASTD---ICN---IQIRSD 180

Query: 181 VPVIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDI 240
              + N  + G    +  P S S NT+                                 
Sbjct: 181 ETKVGN--WMGPAIKMALP-SFSGNTI--------------------------------Y 240

Query: 241 QNILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDHHAGEDAEHEQS 300
            + LL YS          C     VR V+PA VS  L    ++  +++H + E  E+E+ 
Sbjct: 241 NSNLLKYS----------CHLHCRVRPVRPAVVSGALEDETEKFTEQNHTSQESLENERQ 278

Query: 301 LCTSVLLSKPILALEFSCMEMQVQAPAVV 328
           L  +V+LSKPI+AL+F C+ MQV+AP V+
Sbjct: 301 LSKAVMLSKPIIALQFKCLTMQVEAPVVI 278

BLAST of Lsi07G003810 vs. TAIR 10
Match: AT1G28100.2 (unknown protein; Has 64 Blast hits to 64 proteins in 27 species: Archae - 0; Bacteria - 14; Metazoa - 0; Fungi - 6; Plants - 42; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 259.6 bits (662), Expect = 3.5e-69
Identity = 153/329 (46.50%), Postives = 197/329 (59.88%), Query Frame = 0

Query: 1   MEVGEKNCSSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLAN 60
           M+V EK  SSGY KPPWIF+G ALYQ+HLVKA TARA IPKE RLVEAFGYTLGGFFLA+
Sbjct: 1   MDVEEKRVSSGYAKPPWIFKGSALYQIHLVKAATARAFIPKEFRLVEAFGYTLGGFFLAS 60

Query: 61  YEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKRI 120
           Y+DSPAG FDELVVI+GIVWN PTSCAWAA+VLVNS +AC HGRKEVGLPSQVARF+K I
Sbjct: 61  YDDSPAGVFDELVVIAGIVWNPPTSCAWAARVLVNSDEACHHGRKEVGLPSQVARFSKNI 120

Query: 121 EAVPMRQSER--GLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICESKCSELFAS 180
            AVP ++ +R  G L++    + +   ++   V+V+EV    S D   IC     ++ + 
Sbjct: 121 TAVPKQKRDRAFGFLDTFGLGTTLSHPENLMEVKVSEVDSAASTD---ICN---IQIRSD 180

Query: 181 VPVIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKCLSQVIVDI 240
              + N  + G    +  P S S NT+                                 
Sbjct: 181 ETKVGN--WMGPAIKMALP-SFSGNTI--------------------------------Y 240

Query: 241 QNILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDHHAGEDAEHEQS 300
            + LL YS          C     VR V+PA VS  L    ++  +++H + E  E+E+ 
Sbjct: 241 NSNLLKYS----------CHLHCRVRPVRPAVVSGALEDETEKFTEQNHTSQESLENERQ 278

Query: 301 LCTSVLLSKPILALEFSCMEMQVQAPAVV 328
           L  +V+LSKPI+AL+F C+ MQV+AP V+
Sbjct: 301 LSKAVMLSKPIIALQFKCLTMQVEAPVVI 278

BLAST of Lsi07G003810 vs. TAIR 10
Match: AT1G28100.4 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 64 Blast hits to 64 proteins in 27 species: Archae - 0; Bacteria - 14; Metazoa - 0; Fungi - 6; Plants - 42; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 252.3 bits (643), Expect = 5.5e-67
Identity = 153/337 (45.40%), Postives = 197/337 (58.46%), Query Frame = 0

Query: 1   MEVGEKNCSSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLAN 60
           M+V EK  SSGY KPPWIF+G ALYQ+HLVKA TARA IPKE RLVEAFGYTLGGFFLA+
Sbjct: 1   MDVEEKRVSSGYAKPPWIFKGSALYQIHLVKAATARAFIPKEFRLVEAFGYTLGGFFLAS 60

Query: 61  YEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFT--- 120
           Y+DSPAG FDELVVI+GIVWN PTSCAWAA+VLVNS +AC HGRKEVGLPSQVARF+   
Sbjct: 61  YDDSPAGVFDELVVIAGIVWNPPTSCAWAARVLVNSDEACHHGRKEVGLPSQVARFSKVS 120

Query: 121 -----KRIEAVPMRQSER--GLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSIDRLPICES 180
                K I AVP ++ +R  G L++    + +   ++   V+V+EV    S D   IC  
Sbjct: 121 DTLFLKNITAVPKQKRDRAFGFLDTFGLGTTLSHPENLMEVKVSEVDSAASTD---ICN- 180

Query: 181 KCSELFASVPVIANQPYPGSDFNVYFPASSSSNTLTAYGLLVEPITVFLSANGWDQLSKC 240
              ++ +    + N  + G    +  P S S NT+                         
Sbjct: 181 --IQIRSDETKVGN--WMGPAIKMALP-SFSGNTI------------------------- 240

Query: 241 LSQVIVDIQNILLNYSNIPAKFNAGLCFCSLMVRAVKPAAVSVKLPTSIDRAQDRDHHAG 300
                    + LL YS          C     VR V+PA VS  L    ++  +++H + 
Sbjct: 241 -------YNSNLLKYS----------CHLHCRVRPVRPAVVSGALEDETEKFTEQNHTSQ 286

Query: 301 EDAEHEQSLCTSVLLSKPILALEFSCMEMQVQAPAVV 328
           E  E+E+ L  +V+LSKPI+AL+F C+ MQV+AP V+
Sbjct: 301 ESLENERQLSKAVMLSKPIIALQFKCLTMQVEAPVVI 286

BLAST of Lsi07G003810 vs. TAIR 10
Match: AT1G28100.5 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 64 Blast hits to 64 proteins in 27 species: Archae - 0; Bacteria - 14; Metazoa - 0; Fungi - 6; Plants - 42; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 224.6 bits (571), Expect = 1.2e-58
Identity = 110/165 (66.67%), Postives = 130/165 (78.79%), Query Frame = 0

Query: 1   MEVGEKNCSSGYGKPPWIFRGRALYQLHLVKAKTARACIPKELRLVEAFGYTLGGFFLAN 60
           M+V EK  SSGY KPPWIF+G ALYQ+HLVKA TARA IPKE RLVEAFGYTLGGFFLA+
Sbjct: 1   MDVEEKRVSSGYAKPPWIFKGSALYQIHLVKAATARAFIPKEFRLVEAFGYTLGGFFLAS 60

Query: 61  YEDSPAGTFDELVVISGIVWNRPTSCAWAAKVLVNSVDACDHGRKEVGLPSQVARFTKRI 120
           Y+DSPAG FDELVVI+GIVWN PTSCAWAA+VLVNS +AC HGRKEVGLPSQVARF+K I
Sbjct: 61  YDDSPAGVFDELVVIAGIVWNPPTSCAWAARVLVNSDEACHHGRKEVGLPSQVARFSKNI 120

Query: 121 EAVPMRQSER--GLLNSLRGNSNIYDQKSQEHVQVTEVKGPTSID 164
            AVP ++ +R  G L++    + +   ++   V+V+EV    S D
Sbjct: 121 TAVPKQKRDRAFGFLDTFGLGTTLSHPENLMEVKVSEVDSAASTD 165