Lsi07G003260 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G003260
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionWAT1-related protein
Locationchr07: 3468063 .. 3470065 (-)
RNA-Seq ExpressionLsi07G003260
SyntenyLsi07G003260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGCCAGTCCAAATATTTGCCACCGGAATGCAACTTCTGAGCAAAGTTATTCTGAATCATGGCACCTTCATTTTTGCACTCATGGCCTACCGTCATCTCGTCGCCACCCTCTGCGTTGCACCCTTTGCTTTCTTCTTCGAAAGGTCTGTAGCTTCATATACATAATTACATTATTAATTAATATATCCCTAAGTCTTCACATTTAATTTTATAATTAAAGCTACATTCACGTTGATGTTGCCTACTCACTCCATTCTCGTGCTAATTTGGTTGCAGAAGAAACGCAAACAAGTTGAGCTGCCAGGTTTTCTTTTGGCTTTTTCTCAGTGCCTTCACCGGGTACCATCATCATCATTATTATTACTATGCATTATTCATCTTCATCCTTTATATGATCAATAACCTTCTGTTGCGTACGTGTTTCCCCTTAAGGTTTTTTTTCTTTTCTTCCCTTTCAAAAATTTTGGTAGCATATCTGGTCTAACCTAACCTAATTAACTTTAATTTTATTCCATCTCAAAATGGTAACTTTAATTAACTTATAATTAAGGATAATATAATTGATATCATGTCATAAATGAATAAGGCAGATTGTTAGATATAATAGTGACAGAGTAATTAATATATGTATATATTTGCATGCAGGATAACAGCGGCAATGGGACTGTACTACTATGGTCTGCGAGACACGACAGCTACTTATGCTACCAACTTCCTGAACTTGATTCCCGTAGTGACATTTGTAATCTCCTCCATCCTGAGGTATATGTAAACATGGCATGGCCCATATACTGCAGGCCCTTGAGTATATATATATATGTGTGTGTGTGTAGGTTGATAATAATAAATGGTTTTTGTAGGATGGAGAAAGTGAGGTTGAAGAGAAGAGCAGGGCAAATAACAGTAGTGGGAGCAATTTTGTGTGTTGGGGGAGCATTAATTACGAGTCTTTACAAAGGAAAAGGATTCCACATTGGTCATCACGATCATGTTAATAATAATAATAATAACAACAACAATTGTATAATATCTAATATGGAGGCGGGTGGTCGTCACTGGGGACGAGGCACTCTCCTGCTTATTGGCAGCTGCTTCTCTTATGCTACATGGTTTGTTGTCCAAGTAAGTATTATGCCATCTACACTTTCTGTGCAAATCATCAATAATATAATTCTAATAATAATAATGGGGCTACACACACACACACACATGCTACAGGTGAAGTTGCTGAAACTGTTTCCCTCAAACTACTTGGCCACCATGCTAACATGTGTCATAGCATGCATTCAGTCAACACTGCTTGGCTTGTGCCTCGACACCAACAAGGCTTCTTGGAAGTTGGGTTGGGATCTCCAGCTTCTCACCATTTTCTACTCGGTAACCAGAAATTAAATTAAATTCATATATTATTATTATTATTATTATTATTATTATTATTATTATTATATGATATCATTAATTATATATATGGACAGGGAGCACTGGCCACAGCAGCTACTTTTTGCTTGACCACATGGGCAATTTCGATCCAAGGACCCACTTTCCCTCCAATGTTCAACCCTCTCAGTCTAATCTTTGTGGCAATCTCAGAAGCCATTATACTTGGGGAGGAGATTAAAGTGGGCAAGTACGTACGTACGCATGCTGTTGGATTATATATATATATTTAATTATATATTAAGAAAAAAAAAATGGATGGAAGGATATAATATTAATAAATGATGAGTTGAGTAGAGGTTGTTGTATGTTGTTTGAATTGCAGCATATTGGGGACGTGTGTGATGGTAGCGGGGCTGTACTGTTTCTTGTGGGGTAAGACAAAGGAGATGAAGATGAACAAATCAACACATCTCCCGAGATCATCAGCAGCAGCTGCAGTCGTTGCAATTCATCAACCACCAACCACTTCTCAACCTCCGCCTCTGCACTCACCACCTCTACTCCCAACGCCTGCTTCTTCACCCACTAATAACAACGACAATATTAATAATATTAATTAA

mRNA sequence

ATGTTGCCAGTCCAAATATTTGCCACCGGAATGCAACTTCTGAGCAAAGTTATTCTGAATCATGGCACCTTCATTTTTGCACTCATGGCCTACCGTCATCTCGTCGCCACCCTCTGCGTTGCACCCTTTGCTTTCTTCTTCGAAAGAAGAAACGCAAACAAGTTGAGCTGCCAGGTTTTCTTTTGGCTTTTTCTCAGTGCCTTCACCGGGATAACAGCGGCAATGGGACTGTACTACTATGGTCTGCGAGACACGACAGCTACTTATGCTACCAACTTCCTGAACTTGATTCCCGTAGTGACATTTGTAATCTCCTCCATCCTGAGGATGGAGAAAGTGAGGTTGAAGAGAAGAGCAGGGCAAATAACAGTAGTGGGAGCAATTTTGTGTGTTGGGGGAGCATTAATTACGAGTCTTTACAAAGGAAAAGGATTCCACATTGGTCATCACGATCATGTTAATAATAATAATAATAACAACAACAATTGTATAATATCTAATATGGAGGCGGGTGGTCGTCACTGGGGACGAGGCACTCTCCTGCTTATTGGCAGCTGCTTCTCTTATGCTACATGGTTTGTTGTCCAATCAACACTGCTTGGCTTGTGCCTCGACACCAACAAGGCTTCTTGGAAGTTGGGTTGGGATCTCCAGCTTCTCACCATTTTCTACTCGGGAGCACTGGCCACAGCAGCTACTTTTTGCTTGACCACATGGGCAATTTCGATCCAAGGACCCACTTTCCCTCCAATGTTCAACCCTCTCAGTCTAATCTTTGTGGCAATCTCAGAAGCCATTATACTTGGGGAGGAGATTAAAGTGGGCAACATATTGGGGACGTGTGTGATGGTAGCGGGGCTGTACTGTTTCTTGTGGGGTAAGACAAAGGAGATGAAGATGAACAAATCAACACATCTCCCGAGATCATCAGCAGCAGCTGCAGTCGTTGCAATTCATCAACCACCAACCACTTCTCAACCTCCGCCTCTGCACTCACCACCTCTACTCCCAACGCCTGCTTCTTCACCCACTAATAACAACGACAATATTAATAATATTAATTAA

Coding sequence (CDS)

ATGTTGCCAGTCCAAATATTTGCCACCGGAATGCAACTTCTGAGCAAAGTTATTCTGAATCATGGCACCTTCATTTTTGCACTCATGGCCTACCGTCATCTCGTCGCCACCCTCTGCGTTGCACCCTTTGCTTTCTTCTTCGAAAGAAGAAACGCAAACAAGTTGAGCTGCCAGGTTTTCTTTTGGCTTTTTCTCAGTGCCTTCACCGGGATAACAGCGGCAATGGGACTGTACTACTATGGTCTGCGAGACACGACAGCTACTTATGCTACCAACTTCCTGAACTTGATTCCCGTAGTGACATTTGTAATCTCCTCCATCCTGAGGATGGAGAAAGTGAGGTTGAAGAGAAGAGCAGGGCAAATAACAGTAGTGGGAGCAATTTTGTGTGTTGGGGGAGCATTAATTACGAGTCTTTACAAAGGAAAAGGATTCCACATTGGTCATCACGATCATGTTAATAATAATAATAATAACAACAACAATTGTATAATATCTAATATGGAGGCGGGTGGTCGTCACTGGGGACGAGGCACTCTCCTGCTTATTGGCAGCTGCTTCTCTTATGCTACATGGTTTGTTGTCCAATCAACACTGCTTGGCTTGTGCCTCGACACCAACAAGGCTTCTTGGAAGTTGGGTTGGGATCTCCAGCTTCTCACCATTTTCTACTCGGGAGCACTGGCCACAGCAGCTACTTTTTGCTTGACCACATGGGCAATTTCGATCCAAGGACCCACTTTCCCTCCAATGTTCAACCCTCTCAGTCTAATCTTTGTGGCAATCTCAGAAGCCATTATACTTGGGGAGGAGATTAAAGTGGGCAACATATTGGGGACGTGTGTGATGGTAGCGGGGCTGTACTGTTTCTTGTGGGGTAAGACAAAGGAGATGAAGATGAACAAATCAACACATCTCCCGAGATCATCAGCAGCAGCTGCAGTCGTTGCAATTCATCAACCACCAACCACTTCTCAACCTCCGCCTCTGCACTCACCACCTCTACTCCCAACGCCTGCTTCTTCACCCACTAATAACAACGACAATATTAATAATATTAATTAA

Protein sequence

MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVFFWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAGQITVVGAILCVGGALITSLYKGKGFHIGHHDHVNNNNNNNNNCIISNMEAGGRHWGRGTLLLIGSCFSYATWFVVQSTLLGLCLDTNKASWKLGWDLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVGNILGTCVMVAGLYCFLWGKTKEMKMNKSTHLPRSSAAAAVVAIHQPPTTSQPPPLHSPPLLPTPASSPTNNNDNINNIN
Homology
BLAST of Lsi07G003260 vs. ExPASy Swiss-Prot
Match: Q9FGG3 (WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.9e-44
Identity = 107/323 (33.13%), Postives = 174/323 (53.87%), Query Frame = 0

Query: 4   VQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVFFWL 63
           +Q+  T M L+SK + N G   F  + YR   AT+ +AP AFFFER++A  LS   F  +
Sbjct: 15  IQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKSAPPLSFVTFIKI 74

Query: 64  FLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAGQIT 123
           F+ +  G+T ++ L    L  T+AT A      +P +TF ++ +  ME++++K   G   
Sbjct: 75  FMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGMERLKVKSIQGTAK 134

Query: 124 VVGAILCVGGALITSLYKGKGFHI---GHHDHVNNNNNNNNNCIISNMEAGGRHWGRGTL 183
           +VG  +C+GG +I ++YKG    +    H  H   + + NN     ++  G   W +G +
Sbjct: 135 LVGITVCMGGVIILAIYKGPLLKLPLCPHFYHGQEHPHRNNP---GHVSGGSTSWLKGCV 194

Query: 184 LLIGSCFSYATWFV------------------------VQSTLLGLCLDTNKASWKLGWD 243
           L+I S   +  W V                        +QS ++ + L+ + ++WKLGW+
Sbjct: 195 LMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALERDISAWKLGWN 254

Query: 244 LQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVGN 300
           L+L+ + Y G + T   + L +W I  +GP F  MF PLSL+F  +S AI+L E I +G+
Sbjct: 255 LRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSAILLCEIISLGS 314

BLAST of Lsi07G003260 vs. ExPASy Swiss-Prot
Match: Q9ZUS1 (WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 4.3e-43
Identity = 114/336 (33.93%), Postives = 179/336 (53.27%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           M+ +Q+   GM +LSK +LN G   + L+ YRH VAT+ +APFAF+F+++   K++  +F
Sbjct: 19  MVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKVRPKMTLMIF 78

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
           F + L           LYY G++ TTAT+AT   N++P +TFV++ I  +E+V+L+    
Sbjct: 79  FKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLERVKLRCIRS 138

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHHDHVNNNNNNNNNCIISNMEAGGRHWGRGTL 180
              VVG +  VGGA+I +L KG    +     V+ +N    + I S +        +G +
Sbjct: 139 TGKVVGTLATVGGAMIMTLVKGPVLDLFWTKGVSAHNTAGTD-IHSAI--------KGAV 198

Query: 181 LLIGSCFSYATWFVVQSTLL-----------GLCL--------------DTNKASWKLGW 240
           L+   CFSYA + ++Q+  L            +CL                N ++W +GW
Sbjct: 199 LVTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNPSAWAIGW 258

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 300
           D +LLT  YSG + +A  + +    +  +GP F   F+PL +I VAI   II  E++ +G
Sbjct: 259 DTKLLTATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMSTIIFAEQMYLG 318

Query: 301 NILGTCVMVAGLYCFLWGKTKEMKMNKSTHLPRSSA 312
            +LG  V+ AGLY  +WGK K+ K N +  L   SA
Sbjct: 319 RVLGAVVICAGLYLVIWGKGKDYKYNSTLQLDDESA 345

BLAST of Lsi07G003260 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 5.7e-43
Identity = 97/322 (30.12%), Postives = 181/322 (56.21%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           M+ +Q    GM +++K+ LN G   + L+ YRH +AT  +APFAFFFER+   K++  +F
Sbjct: 22  MISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKITFSIF 81

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
             LF+    G       YY GL+ T+ T++    N++P +TF+++ + RME + LK+   
Sbjct: 82  MQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLWC 141

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHHDHVNNNNNNNNNCIISNMEAGGRHWGRGTL 180
           Q  + G ++ V GA++ ++YKG    +    +++  ++++ N   S   +  + + +G++
Sbjct: 142 QAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFLKGSI 201

Query: 181 LLIGSCFSYATWFVVQSTLLG------------LC-------------LDTNKASWKLGW 240
           LLI +  ++A+ FV+Q+ +L             +C             ++ N ++W++GW
Sbjct: 202 LLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAWRIGW 261

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 298
           D+ LL   YSG +A++ ++ +    +  +GP F   F+PL ++ VA+  + +L E+I +G
Sbjct: 262 DMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIFLG 321

BLAST of Lsi07G003260 vs. ExPASy Swiss-Prot
Match: Q8GXB4 (WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 6.9e-41
Identity = 107/327 (32.72%), Postives = 176/327 (53.82%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           M+ VQI   GM + SK+ +  G     L+AYR + AT+   P AFF ER+   K++ ++ 
Sbjct: 12  MVLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATFPVAFFLERKTRPKITLRIL 71

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
             +F  + TG T    LY+ GL++++ T A    NL+P VTF++++I R E V +K+ +G
Sbjct: 72  VQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVTFLLAAIFRQETVGIKKASG 131

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIG----HHDHVNNNNNNNNNCIISNMEAGGRHWG 180
           Q  V+G ++CV GA++ S Y G    IG    H  +  N   + ++       +G  ++ 
Sbjct: 132 QAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENITKHGSS-------SGHSNFF 191

Query: 181 RGTLLLIGSCFSYATWFVVQ------------STLLGLCL-------------DTNKASW 240
            G  L++ +  S+A WF++Q            STLL +CL             D   + W
Sbjct: 192 LGPFLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLL-MCLMGSIQCGAIALISDHTISDW 251

Query: 241 KLGWDLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEE 299
            L   L+ ++  Y+G +A+A  FCL +WA+  +GP +  +F+PL L+ VAI    +L E+
Sbjct: 252 SLSSPLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWALLEEK 311

BLAST of Lsi07G003260 vs. ExPASy Swiss-Prot
Match: Q94AP3 (Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana OX=3702 GN=WAT1 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 9.1e-41
Identity = 100/324 (30.86%), Postives = 169/324 (52.16%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           ML +Q    G  ++S+  LN G        YR+++A L + PFA+F E++    ++    
Sbjct: 24  MLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNIIALLLLLPFAYFLEKKERPAITLNFL 83

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
              F  A  GITA  G Y  GL +T+ T+A++  N +P +TF+++++LR+EKVR+ RR G
Sbjct: 84  IQFFFLALIGITANQGFYLLGLDNTSPTFASSMQNSVPAITFLMAALLRIEKVRINRRDG 143

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHHDHVNNNNNNNNNCIISNM-EAGGRHWGRGT 180
              ++G  LCV GA + +LYKG   +     H++ +    N+ +++ +  A  ++W  G 
Sbjct: 144 ISKILGTALCVAGASVITLYKGPTIYT-PASHLHAHLLTTNSAVLAPLGNAAPKNWTLGC 203

Query: 181 LLLIGSCFSYATWFV------------------------VQSTLLGLCLDTNKASWKLGW 240
           + LIG C S++ W V                        +Q  ++    + +  +W    
Sbjct: 204 IYLIGHCLSWSGWLVFQAPVLKSYPARLSVTSYTCFFGIIQFLIIAAFCERDSQAWVFHS 263

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 300
             +L TI Y+G +A+   F +  W I   GP F  ++ P+  + VAI  +I LGEE  +G
Sbjct: 264 GWELFTILYAGIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASIALGEEFYLG 323

BLAST of Lsi07G003260 vs. ExPASy TrEMBL
Match: A0A6J1E7Y3 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111430706 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 4.1e-145
Identity = 284/371 (76.55%), Postives = 308/371 (83.02%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           MLPVQIFATGMQLLSKVILNHGTF+FALMAYRHLVA LCVAPFAFFFERRNANKLS QV 
Sbjct: 19  MLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAPFAFFFERRNANKLSWQVL 78

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
           FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISS+LR+EKV LKRRAG
Sbjct: 79  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSMLRIEKVSLKRRAG 138

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHH-DHVNNNNNNNNNCIISNMEAGGRHWGRGT 180
           +ITVVGAILCVGG +ITS+Y+GKGFHIGHH  HVN+N NNN +        GGRHWGRGT
Sbjct: 139 RITVVGAILCVGGVVITSVYRGKGFHIGHHVAHVNDNTNNNAS------NEGGRHWGRGT 198

Query: 181 LLLIGSCFSYATWFVV------------------------QSTLLGLCLDTNKASWKLGW 240
           LLL+GSCFSY+TWFVV                        QSTLLGLCLDTN ASWKLGW
Sbjct: 199 LLLLGSCFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLCLDTNNASWKLGW 258

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 300
           DLQLLTI YSGALATAATFCL TWAIS+QGPTFPPMFNPL+LIFVAISE IILGEEIKVG
Sbjct: 259 DLQLLTILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVAISEGIILGEEIKVG 318

Query: 301 NILGTCVMVAGLYCFLWGKTKEMKMNKSTHLPRSSAAAAVVAIHQPPTTSQPPPLHSPPL 347
           ++LGT VMVAGLYCFLWGKTKE+K  KS HLPR++AAA  +AI     TS+P PL S  +
Sbjct: 319 SMLGTGVMVAGLYCFLWGKTKEVK--KSAHLPRATAAA--LAIEAATATSEPAPLPSAAV 378

BLAST of Lsi07G003260 vs. ExPASy TrEMBL
Match: A0A6J1E4N2 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111430706 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 4.1e-145
Identity = 284/371 (76.55%), Postives = 308/371 (83.02%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           MLPVQIFATGMQLLSKVILNHGTF+FALMAYRHLVA LCVAPFAFFFERRNANKLS QV 
Sbjct: 1   MLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAPFAFFFERRNANKLSWQVL 60

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
           FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISS+LR+EKV LKRRAG
Sbjct: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSMLRIEKVSLKRRAG 120

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHH-DHVNNNNNNNNNCIISNMEAGGRHWGRGT 180
           +ITVVGAILCVGG +ITS+Y+GKGFHIGHH  HVN+N NNN +        GGRHWGRGT
Sbjct: 121 RITVVGAILCVGGVVITSVYRGKGFHIGHHVAHVNDNTNNNAS------NEGGRHWGRGT 180

Query: 181 LLLIGSCFSYATWFVV------------------------QSTLLGLCLDTNKASWKLGW 240
           LLL+GSCFSY+TWFVV                        QSTLLGLCLDTN ASWKLGW
Sbjct: 181 LLLLGSCFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLCLDTNNASWKLGW 240

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 300
           DLQLLTI YSGALATAATFCL TWAIS+QGPTFPPMFNPL+LIFVAISE IILGEEIKVG
Sbjct: 241 DLQLLTILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVAISEGIILGEEIKVG 300

Query: 301 NILGTCVMVAGLYCFLWGKTKEMKMNKSTHLPRSSAAAAVVAIHQPPTTSQPPPLHSPPL 347
           ++LGT VMVAGLYCFLWGKTKE+K  KS HLPR++AAA  +AI     TS+P PL S  +
Sbjct: 301 SMLGTGVMVAGLYCFLWGKTKEVK--KSAHLPRATAAA--LAIEAATATSEPAPLPSAAV 360

BLAST of Lsi07G003260 vs. ExPASy TrEMBL
Match: A0A6J1J9I9 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111482962 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 7.7e-144
Identity = 284/371 (76.55%), Postives = 307/371 (82.75%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           MLPVQIFATGMQLLSKVILNHGTF+FALMAYRHLVA LCVAPFA FFERRNANKLS QV 
Sbjct: 1   MLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAPFA-FFERRNANKLSWQVL 60

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
           FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISS+LR+EKV LKRRAG
Sbjct: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSMLRIEKVSLKRRAG 120

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHH-DHVNNNNNNNNNCIISNMEAGGRHWGRGT 180
           +ITVVGAILCVGG +ITS+Y+GKGFHIGHH  HVN+N NNN +        GGRHWGRGT
Sbjct: 121 RITVVGAILCVGGVVITSVYRGKGFHIGHHVAHVNDNTNNNTS------NEGGRHWGRGT 180

Query: 181 LLLIGSCFSYATWFVV------------------------QSTLLGLCLDTNKASWKLGW 240
           LLL+GSCFSY+TWFVV                        QSTLLGLCLDTN ASWKLGW
Sbjct: 181 LLLLGSCFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLCLDTNNASWKLGW 240

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 300
           DLQLLTI YSGALATAATFCL TWAIS+QGPTFPPMFNPL+LIFVAISE IILGEEIKVG
Sbjct: 241 DLQLLTILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVAISEGIILGEEIKVG 300

Query: 301 NILGTCVMVAGLYCFLWGKTKEMKMNKSTHLPRSSAAAAVVAIHQPPTTSQPPPLHSPPL 347
           ++LGT VMVAGLYCFLWGKTKEMK  KS HLPR++AAA  +AI     TS+P PL S  +
Sbjct: 301 SMLGTGVMVAGLYCFLWGKTKEMK--KSAHLPRATAAA--LAIEAATATSEPAPLPSAAV 360

BLAST of Lsi07G003260 vs. ExPASy TrEMBL
Match: A0A6J1JBI3 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111482962 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 7.7e-144
Identity = 284/371 (76.55%), Postives = 307/371 (82.75%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           MLPVQIFATGMQLLSKVILNHGTF+FALMAYRHLVA LCVAPFA FFERRNANKLS QV 
Sbjct: 19  MLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAPFA-FFERRNANKLSWQVL 78

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
           FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISS+LR+EKV LKRRAG
Sbjct: 79  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSMLRIEKVSLKRRAG 138

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHH-DHVNNNNNNNNNCIISNMEAGGRHWGRGT 180
           +ITVVGAILCVGG +ITS+Y+GKGFHIGHH  HVN+N NNN +        GGRHWGRGT
Sbjct: 139 RITVVGAILCVGGVVITSVYRGKGFHIGHHVAHVNDNTNNNTS------NEGGRHWGRGT 198

Query: 181 LLLIGSCFSYATWFVV------------------------QSTLLGLCLDTNKASWKLGW 240
           LLL+GSCFSY+TWFVV                        QSTLLGLCLDTN ASWKLGW
Sbjct: 199 LLLLGSCFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLCLDTNNASWKLGW 258

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 300
           DLQLLTI YSGALATAATFCL TWAIS+QGPTFPPMFNPL+LIFVAISE IILGEEIKVG
Sbjct: 259 DLQLLTILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVAISEGIILGEEIKVG 318

Query: 301 NILGTCVMVAGLYCFLWGKTKEMKMNKSTHLPRSSAAAAVVAIHQPPTTSQPPPLHSPPL 347
           ++LGT VMVAGLYCFLWGKTKEMK  KS HLPR++AAA  +AI     TS+P PL S  +
Sbjct: 319 SMLGTGVMVAGLYCFLWGKTKEMK--KSAHLPRATAAA--LAIEAATATSEPAPLPSAAV 378

BLAST of Lsi07G003260 vs. ExPASy TrEMBL
Match: A0A6J1CI51 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111011392 PE=3 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 1.8e-129
Identity = 259/376 (68.88%), Postives = 291/376 (77.39%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           ML VQ+FATGMQLLSK+ILN GTFIFALMAYRH+VA LCVAPFAFFF+R +ANK S QV 
Sbjct: 24  MLLVQLFATGMQLLSKLILNQGTFIFALMAYRHVVAALCVAPFAFFFDRSHANKFSWQVL 83

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
           FWLFLSAFTGITAAMG+YYYGLRDTTATYATNFLNLIPVVTFVISS+L MEKV ++RRAG
Sbjct: 84  FWLFLSAFTGITAAMGMYYYGLRDTTATYATNFLNLIPVVTFVISSVLGMEKVSVRRRAG 143

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHHDHVNNNNNNNNNCIISNMEAGGR-HWGRGT 180
           ++ +VGAILCVGGALIT  YKGKGFHIGHH    + +      I + +EA    HWGRGT
Sbjct: 144 KVKIVGAILCVGGALITVFYKGKGFHIGHHAIAGHRDVAAPAVIFNEIEADAEAHWGRGT 203

Query: 181 LLLIGSCFSYATWFVV------------------------QSTLLGLCLDTNKASWKLGW 240
           LLL+GSCF YA WFVV                        QST LGLCLD NKA+W LGW
Sbjct: 204 LLLLGSCFCYAAWFVVQVKLLKLFPSKYLATMLTCVIACIQSTALGLCLDRNKAAWTLGW 263

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 300
           DLQLLTI YSGALATAATFCL TWAISIQGPTFPPMFNP++LI VAISE IILGEEI+VG
Sbjct: 264 DLQLLTILYSGALATAATFCLMTWAISIQGPTFPPMFNPVTLILVAISEGIILGEEIRVG 323

Query: 301 NILGTCVMVAGLYCFLWGKTKEMKMNKSTHLPRSSAAAAVVAIHQPPTTSQPPPLHSPPL 352
           NI+GT VMVAGLYCFLWGKTKEMK  KS+HLPR++A A   A      TS+P PL S  +
Sbjct: 324 NIVGTAVMVAGLYCFLWGKTKEMK--KSSHLPRAAAGAVEAA----TATSEPAPLQSAAV 383

BLAST of Lsi07G003260 vs. NCBI nr
Match: XP_038877968.1 (WAT1-related protein At5g64700-like [Benincasa hispida])

HSP 1 Score: 604.4 bits (1557), Expect = 6.4e-169
Identity = 317/366 (86.61%), Postives = 328/366 (89.62%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           MLPVQIFATGMQ+LSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF
Sbjct: 19  MLPVQIFATGMQVLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 78

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
           FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIP VTFVISSILR+EKVRLKRRAG
Sbjct: 79  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPAVTFVISSILRIEKVRLKRRAG 138

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHHDHVNNNNNNNNNCIISNMEAGGRHWGRGTL 180
           Q+TVVGAILCVGGALITSLYKGKGFHIGHHD        ++NCIISNMEAGGRHWGRGTL
Sbjct: 139 QVTVVGAILCVGGALITSLYKGKGFHIGHHD--------DDNCIISNMEAGGRHWGRGTL 198

Query: 181 LLIGSCFSYATWFVV------------------------QSTLLGLCLDTNKASWKLGWD 240
           LL+ SCFSYATWFVV                        QSTLLGLCLDTNKASWKLGWD
Sbjct: 199 LLVASCFSYATWFVVQVKLVKVFPSNYLATMLTCVIACIQSTLLGLCLDTNKASWKLGWD 258

Query: 241 LQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVGN 300
           LQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPL+LIFVAISEAIILGEEIKVGN
Sbjct: 259 LQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLALIFVAISEAIILGEEIKVGN 318

Query: 301 ILGTCVMVAGLYCFLWGKTKEMKMNK-STHLPRSSAAAAVVAIHQPPTTSQPPPLHSPPL 342
           ILGTCVMVAGLYCFLWGKTK+MKMN+ STHLPRS+AAA  VAIH PPTTSQPPPLHSPPL
Sbjct: 319 ILGTCVMVAGLYCFLWGKTKDMKMNELSTHLPRSAAAA--VAIHLPPTTSQPPPLHSPPL 374

BLAST of Lsi07G003260 vs. NCBI nr
Match: XP_023553116.1 (WAT1-related protein At5g64700-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 527.3 bits (1357), Expect = 1.0e-145
Identity = 285/371 (76.82%), Postives = 308/371 (83.02%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           MLPVQIFATGMQLLSKVILNHGTF+FALMAYRHLVA LCVAPFAFFFERRNANKLS QV 
Sbjct: 19  MLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAPFAFFFERRNANKLSWQVL 78

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
           FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISS+LR+EKV LKRRAG
Sbjct: 79  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSMLRIEKVSLKRRAG 138

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHHD-HVNNNNNNNNNCIISNMEAGGRHWGRGT 180
           +ITVVGAILCVGG +ITS+Y+GKGFHIGHHD HVN+N NNN +        GGRHWGRGT
Sbjct: 139 RITVVGAILCVGGVVITSVYRGKGFHIGHHDAHVNDNTNNNAS------NEGGRHWGRGT 198

Query: 181 LLLIGSCFSYATWFVV------------------------QSTLLGLCLDTNKASWKLGW 240
           LLL+GSCFSY+TWFVV                        QSTLLGLCLDTN ASWKLGW
Sbjct: 199 LLLLGSCFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLCLDTNNASWKLGW 258

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 300
           DLQLLTI YSGALATAATFCL TWAIS+QGPTFPPMFNPL+LIFV ISE IILGEEIKVG
Sbjct: 259 DLQLLTILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVGISEGIILGEEIKVG 318

Query: 301 NILGTCVMVAGLYCFLWGKTKEMKMNKSTHLPRSSAAAAVVAIHQPPTTSQPPPLHSPPL 347
           ++LGT VMVAGLYCFLWGKTKEMK  KS HLPR++AAA  +AI     TS+P PL S  +
Sbjct: 319 SMLGTGVMVAGLYCFLWGKTKEMK--KSAHLPRATAAA--LAIEAATATSEPAPLPSAAV 378

BLAST of Lsi07G003260 vs. NCBI nr
Match: KAG6576614.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014666.1 WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 525.8 bits (1353), Expect = 2.9e-145
Identity = 285/371 (76.82%), Postives = 308/371 (83.02%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           MLPVQIFATGMQLLSKVILNHGTF+FALMAYRHLVA LCVAPFAFFFERRNANKLS QV 
Sbjct: 19  MLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAPFAFFFERRNANKLSWQVL 78

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
           FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISS+LR+EKV LKRRAG
Sbjct: 79  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSMLRIEKVSLKRRAG 138

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHH-DHVNNNNNNNNNCIISNMEAGGRHWGRGT 180
           +ITVVGAILCVGG +ITS+Y+GKGFHIGHH  HVN+N NNN +        GGRHWGRGT
Sbjct: 139 RITVVGAILCVGGVVITSVYRGKGFHIGHHVAHVNDNTNNNAS------NEGGRHWGRGT 198

Query: 181 LLLIGSCFSYATWFVV------------------------QSTLLGLCLDTNKASWKLGW 240
           LLL+GSCFSY+TWFVV                        QSTLLGLCLDTN ASWKLGW
Sbjct: 199 LLLLGSCFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLCLDTNNASWKLGW 258

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 300
           DLQLLTI YSGALATAATFCL TWAIS+QGPTFPPMFNPL+LIFVAISE IILGEEIKVG
Sbjct: 259 DLQLLTILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVAISEGIILGEEIKVG 318

Query: 301 NILGTCVMVAGLYCFLWGKTKEMKMNKSTHLPRSSAAAAVVAIHQPPTTSQPPPLHSPPL 347
           ++LGT VMVAGLYCFLWGKTKEMK  KS HLPR++AAA  +AI     TS+P PL S  +
Sbjct: 319 SMLGTGVMVAGLYCFLWGKTKEMK--KSAHLPRATAAA--LAIEAATATSEPAPLPSAAV 378

BLAST of Lsi07G003260 vs. NCBI nr
Match: XP_022922849.1 (WAT1-related protein At5g64700-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 524.2 bits (1349), Expect = 8.5e-145
Identity = 284/371 (76.55%), Postives = 308/371 (83.02%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           MLPVQIFATGMQLLSKVILNHGTF+FALMAYRHLVA LCVAPFAFFFERRNANKLS QV 
Sbjct: 1   MLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAPFAFFFERRNANKLSWQVL 60

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
           FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISS+LR+EKV LKRRAG
Sbjct: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSMLRIEKVSLKRRAG 120

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHH-DHVNNNNNNNNNCIISNMEAGGRHWGRGT 180
           +ITVVGAILCVGG +ITS+Y+GKGFHIGHH  HVN+N NNN +        GGRHWGRGT
Sbjct: 121 RITVVGAILCVGGVVITSVYRGKGFHIGHHVAHVNDNTNNNAS------NEGGRHWGRGT 180

Query: 181 LLLIGSCFSYATWFVV------------------------QSTLLGLCLDTNKASWKLGW 240
           LLL+GSCFSY+TWFVV                        QSTLLGLCLDTN ASWKLGW
Sbjct: 181 LLLLGSCFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLCLDTNNASWKLGW 240

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 300
           DLQLLTI YSGALATAATFCL TWAIS+QGPTFPPMFNPL+LIFVAISE IILGEEIKVG
Sbjct: 241 DLQLLTILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVAISEGIILGEEIKVG 300

Query: 301 NILGTCVMVAGLYCFLWGKTKEMKMNKSTHLPRSSAAAAVVAIHQPPTTSQPPPLHSPPL 347
           ++LGT VMVAGLYCFLWGKTKE+K  KS HLPR++AAA  +AI     TS+P PL S  +
Sbjct: 301 SMLGTGVMVAGLYCFLWGKTKEVK--KSAHLPRATAAA--LAIEAATATSEPAPLPSAAV 360

BLAST of Lsi07G003260 vs. NCBI nr
Match: XP_022922848.1 (WAT1-related protein At5g64700-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 524.2 bits (1349), Expect = 8.5e-145
Identity = 284/371 (76.55%), Postives = 308/371 (83.02%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           MLPVQIFATGMQLLSKVILNHGTF+FALMAYRHLVA LCVAPFAFFFERRNANKLS QV 
Sbjct: 19  MLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAPFAFFFERRNANKLSWQVL 78

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
           FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISS+LR+EKV LKRRAG
Sbjct: 79  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSMLRIEKVSLKRRAG 138

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHH-DHVNNNNNNNNNCIISNMEAGGRHWGRGT 180
           +ITVVGAILCVGG +ITS+Y+GKGFHIGHH  HVN+N NNN +        GGRHWGRGT
Sbjct: 139 RITVVGAILCVGGVVITSVYRGKGFHIGHHVAHVNDNTNNNAS------NEGGRHWGRGT 198

Query: 181 LLLIGSCFSYATWFVV------------------------QSTLLGLCLDTNKASWKLGW 240
           LLL+GSCFSY+TWFVV                        QSTLLGLCLDTN ASWKLGW
Sbjct: 199 LLLLGSCFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLCLDTNNASWKLGW 258

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 300
           DLQLLTI YSGALATAATFCL TWAIS+QGPTFPPMFNPL+LIFVAISE IILGEEIKVG
Sbjct: 259 DLQLLTILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVAISEGIILGEEIKVG 318

Query: 301 NILGTCVMVAGLYCFLWGKTKEMKMNKSTHLPRSSAAAAVVAIHQPPTTSQPPPLHSPPL 347
           ++LGT VMVAGLYCFLWGKTKE+K  KS HLPR++AAA  +AI     TS+P PL S  +
Sbjct: 319 SMLGTGVMVAGLYCFLWGKTKEVK--KSAHLPRATAAA--LAIEAATATSEPAPLPSAAV 378

BLAST of Lsi07G003260 vs. TAIR 10
Match: AT5G64700.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.8e-45
Identity = 107/323 (33.13%), Postives = 174/323 (53.87%), Query Frame = 0

Query: 4   VQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVFFWL 63
           +Q+  T M L+SK + N G   F  + YR   AT+ +AP AFFFER++A  LS   F  +
Sbjct: 15  IQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKSAPPLSFVTFIKI 74

Query: 64  FLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAGQIT 123
           F+ +  G+T ++ L    L  T+AT A      +P +TF ++ +  ME++++K   G   
Sbjct: 75  FMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGMERLKVKSIQGTAK 134

Query: 124 VVGAILCVGGALITSLYKGKGFHI---GHHDHVNNNNNNNNNCIISNMEAGGRHWGRGTL 183
           +VG  +C+GG +I ++YKG    +    H  H   + + NN     ++  G   W +G +
Sbjct: 135 LVGITVCMGGVIILAIYKGPLLKLPLCPHFYHGQEHPHRNNP---GHVSGGSTSWLKGCV 194

Query: 184 LLIGSCFSYATWFV------------------------VQSTLLGLCLDTNKASWKLGWD 243
           L+I S   +  W V                        +QS ++ + L+ + ++WKLGW+
Sbjct: 195 LMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALERDISAWKLGWN 254

Query: 244 LQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVGN 300
           L+L+ + Y G + T   + L +W I  +GP F  MF PLSL+F  +S AI+L E I +G+
Sbjct: 255 LRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSAILLCEIISLGS 314

BLAST of Lsi07G003260 vs. TAIR 10
Match: AT2G37460.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 176.8 bits (447), Expect = 3.1e-44
Identity = 114/336 (33.93%), Postives = 179/336 (53.27%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           M+ +Q+   GM +LSK +LN G   + L+ YRH VAT+ +APFAF+F+++   K++  +F
Sbjct: 19  MVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKVRPKMTLMIF 78

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
           F + L           LYY G++ TTAT+AT   N++P +TFV++ I  +E+V+L+    
Sbjct: 79  FKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLERVKLRCIRS 138

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHHDHVNNNNNNNNNCIISNMEAGGRHWGRGTL 180
              VVG +  VGGA+I +L KG    +     V+ +N    + I S +        +G +
Sbjct: 139 TGKVVGTLATVGGAMIMTLVKGPVLDLFWTKGVSAHNTAGTD-IHSAI--------KGAV 198

Query: 181 LLIGSCFSYATWFVVQSTLL-----------GLCL--------------DTNKASWKLGW 240
           L+   CFSYA + ++Q+  L            +CL                N ++W +GW
Sbjct: 199 LVTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNPSAWAIGW 258

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 300
           D +LLT  YSG + +A  + +    +  +GP F   F+PL +I VAI   II  E++ +G
Sbjct: 259 DTKLLTATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMSTIIFAEQMYLG 318

Query: 301 NILGTCVMVAGLYCFLWGKTKEMKMNKSTHLPRSSA 312
            +LG  V+ AGLY  +WGK K+ K N +  L   SA
Sbjct: 319 RVLGAVVICAGLYLVIWGKGKDYKYNSTLQLDDESA 345

BLAST of Lsi07G003260 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 176.4 bits (446), Expect = 4.0e-44
Identity = 97/322 (30.12%), Postives = 181/322 (56.21%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           M+ +Q    GM +++K+ LN G   + L+ YRH +AT  +APFAFFFER+   K++  +F
Sbjct: 22  MISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKITFSIF 81

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
             LF+    G       YY GL+ T+ T++    N++P +TF+++ + RME + LK+   
Sbjct: 82  MQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLWC 141

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHHDHVNNNNNNNNNCIISNMEAGGRHWGRGTL 180
           Q  + G ++ V GA++ ++YKG    +    +++  ++++ N   S   +  + + +G++
Sbjct: 142 QAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFLKGSI 201

Query: 181 LLIGSCFSYATWFVVQSTLLG------------LC-------------LDTNKASWKLGW 240
           LLI +  ++A+ FV+Q+ +L             +C             ++ N ++W++GW
Sbjct: 202 LLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAWRIGW 261

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 298
           D+ LL   YSG +A++ ++ +    +  +GP F   F+PL ++ VA+  + +L E+I +G
Sbjct: 262 DMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIFLG 321

BLAST of Lsi07G003260 vs. TAIR 10
Match: AT1G09380.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 169.5 bits (428), Expect = 4.9e-42
Identity = 107/327 (32.72%), Postives = 176/327 (53.82%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           M+ VQI   GM + SK+ +  G     L+AYR + AT+   P AFF ER+   K++ ++ 
Sbjct: 12  MVLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATFPVAFFLERKTRPKITLRIL 71

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
             +F  + TG T    LY+ GL++++ T A    NL+P VTF++++I R E V +K+ +G
Sbjct: 72  VQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVTFLLAAIFRQETVGIKKASG 131

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIG----HHDHVNNNNNNNNNCIISNMEAGGRHWG 180
           Q  V+G ++CV GA++ S Y G    IG    H  +  N   + ++       +G  ++ 
Sbjct: 132 QAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENITKHGSS-------SGHSNFF 191

Query: 181 RGTLLLIGSCFSYATWFVVQ------------STLLGLCL-------------DTNKASW 240
            G  L++ +  S+A WF++Q            STLL +CL             D   + W
Sbjct: 192 LGPFLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLL-MCLMGSIQCGAIALISDHTISDW 251

Query: 241 KLGWDLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEE 299
            L   L+ ++  Y+G +A+A  FCL +WA+  +GP +  +F+PL L+ VAI    +L E+
Sbjct: 252 SLSSPLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWALLEEK 311

BLAST of Lsi07G003260 vs. TAIR 10
Match: AT1G75500.1 (Walls Are Thin 1 )

HSP 1 Score: 169.1 bits (427), Expect = 6.4e-42
Identity = 100/324 (30.86%), Postives = 169/324 (52.16%), Query Frame = 0

Query: 1   MLPVQIFATGMQLLSKVILNHGTFIFALMAYRHLVATLCVAPFAFFFERRNANKLSCQVF 60
           ML +Q    G  ++S+  LN G        YR+++A L + PFA+F E++    ++    
Sbjct: 24  MLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNIIALLLLLPFAYFLEKKERPAITLNFL 83

Query: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEKVRLKRRAG 120
              F  A  GITA  G Y  GL +T+ T+A++  N +P +TF+++++LR+EKVR+ RR G
Sbjct: 84  IQFFFLALIGITANQGFYLLGLDNTSPTFASSMQNSVPAITFLMAALLRIEKVRINRRDG 143

Query: 121 QITVVGAILCVGGALITSLYKGKGFHIGHHDHVNNNNNNNNNCIISNM-EAGGRHWGRGT 180
              ++G  LCV GA + +LYKG   +     H++ +    N+ +++ +  A  ++W  G 
Sbjct: 144 ISKILGTALCVAGASVITLYKGPTIYT-PASHLHAHLLTTNSAVLAPLGNAAPKNWTLGC 203

Query: 181 LLLIGSCFSYATWFV------------------------VQSTLLGLCLDTNKASWKLGW 240
           + LIG C S++ W V                        +Q  ++    + +  +W    
Sbjct: 204 IYLIGHCLSWSGWLVFQAPVLKSYPARLSVTSYTCFFGIIQFLIIAAFCERDSQAWVFHS 263

Query: 241 DLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLSLIFVAISEAIILGEEIKVG 300
             +L TI Y+G +A+   F +  W I   GP F  ++ P+  + VAI  +I LGEE  +G
Sbjct: 264 GWELFTILYAGIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASIALGEEFYLG 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FGG33.9e-4433.13WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2... [more]
Q9ZUS14.3e-4333.93WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2... [more]
Q9FL415.7e-4330.12WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2... [more]
Q8GXB46.9e-4132.72WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2... [more]
Q94AP39.1e-4130.86Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana OX=3702 GN=WAT1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E7Y34.1e-14576.55WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111430706 PE=3 SV=1[more]
A0A6J1E4N24.1e-14576.55WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111430706 PE=3 SV=1[more]
A0A6J1J9I97.7e-14476.55WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111482962 PE=3 SV=1[more]
A0A6J1JBI37.7e-14476.55WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111482962 PE=3 SV=1[more]
A0A6J1CI511.8e-12968.88WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111011392 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038877968.16.4e-16986.61WAT1-related protein At5g64700-like [Benincasa hispida][more]
XP_023553116.11.0e-14576.82WAT1-related protein At5g64700-like [Cucurbita pepo subsp. pepo][more]
KAG6576614.12.9e-14576.82WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG701466... [more]
XP_022922849.18.5e-14576.55WAT1-related protein At5g64700-like isoform X2 [Cucurbita moschata][more]
XP_022922848.18.5e-14576.55WAT1-related protein At5g64700-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G64700.12.8e-4533.13nodulin MtN21 /EamA-like transporter family protein [more]
AT2G37460.13.1e-4433.93nodulin MtN21 /EamA-like transporter family protein [more]
AT5G07050.14.0e-4430.12nodulin MtN21 /EamA-like transporter family protein [more]
AT1G09380.14.9e-4232.72nodulin MtN21 /EamA-like transporter family protein [more]
AT1G75500.16.4e-4230.86Walls Are Thin 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 10..131
e-value: 2.2E-14
score: 53.8
coord: 218..291
e-value: 6.0E-8
score: 33.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 322..354
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 322..340
NoneNo IPR availablePANTHERPTHR31218:SF233WAT1-RELATED PROTEINcoord: 195..305
NoneNo IPR availablePANTHERPTHR31218:SF233WAT1-RELATED PROTEINcoord: 3..199
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 209..295
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 31..139
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 3..199
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 195..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G003260.1Lsi07G003260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity