Lsi07G002540 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi07G002540
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Prolyl-tRNA synthetase
Location	chr07: 2795962 .. 2797216 (-)
RNA-Seq Expression	Lsi07G002540
Synteny	Lsi07G002540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGCCACGCTGGTTCCGTTCGCCACCACTAGCTGGTTGCTGGCTGCCTGGCTTGGTCCGCGGAGGCCGAAATTCGTGCCGTGACGACAACTCTAAGAATCGAGTTTTGGAAGGAGCTATCACGCCTCAGTTACTGGACTTCAATCCATGGTAGCCAACGCCGAGCTCACGGAATATGGTCATGTTCGAGTTACCATGGTTATTCGTCCCTATGGCTCCGCCATTTTCGAAGCAGATCAGGTGGTGGAAGCTTCAATTTATATTTATTTTGACTAGATCCTCTATCAGTAGATTTAATTTATTCATGTATGTGTACTCGGATGATGATTTCCTTTTGCTACAGGGTTATTTGAATGTTAAGTTCAAGGAGACCAATCACAGGAAGGAACATATACTTTCCACAGGTATGGCTCTGGTATACTTCAAAATTCTCATATGAATGAATATATGCAATTTCTAAATAAAACGTAGTTTCTGATTTGAAACTAAGCCTATCCATTTATGAACTAAATCGTGTGGAACTTGTTGATTCTGAATGCCAGATTGTCGTGAATAGCAACAATACGCTTTACACATGAAGTCGTAAGTTTAACTTTGAACTGTTGAGTATTTGACTAACTGACATTGTTGCTAGAATTATAAGCTAGGTTTATTTCATTAGATACATGAATGTGTGTTCATGTACTCTCTTTAATTTGTGCCTTTTTCAATGCCTTTTGGAGTGGTGTATAAAGGTTCATTTTCCACATTTGTGATCAAGCGAGATTCTCTTCTGTATTGCAAGAGAACAATTTCTTCTCTTCATATCGTAAAATTTAAAGTAAATATTATCGTGCATTTATCTATACTTCCAACATAGTGTAAATTGATGGTTACAAAGTCAATTTTGTCATTGCAATGTTCAGTGTAGCAATAACAATTTATTTAAAGTTTAAAACATATATGATATCTTGTATGTAAGTTCCCAACATTGGAGTTGTTAAGACAAGCATTGCAGTGAAGACTATGATATGTGGTTAGTTATAGTTGACGATGATTCCTTTCAGCTAACCCGGGCTCTTAAACATCTATTTGCTAGAAATGAAAATTTGATTGTTGATCCCAGTTTAAACCATATTCATTCATTGAGAAAGAAGCTAGTCATGTTGCAGTTAGCTCTTGTAACTGTAGGAGAGAAAGAACTTGAAGAAAAGCTTGGGGAGTACATAGACCCAGTTAAGCTAATGGCCCATTTCTGGGCGTTCATTGCTCATTAG

mRNA sequence

AAGCCACGCTGGTTCCGTTCGCCACCACTAGCTGGTTGCTGGCTGCCTGGCTTGGTCCGCGGAGGCCGAAATTCGTGCCGTGACGACAACTCTAAGAATCGAGTTTTGGAAGGAGCTATCACGCCTCAGTTACTGGACTTCAATCCATGGTAGCCAACGCCGAGCTCACGGAATATGGTCATGTTCGAGTTACCATGGTTATTCGTCCCTATGGCTCCGCCATTTTCGAAGCAGATCAGGGTTATTTGAATGTTAAGTTCAAGGAGACCAATCACAGGAAGGAACATATACTTTCCACAGACAAGCATTGCAGTGAAGACTATGATATGTGGTTAGTTATAGTTGACGATGATTCCTTTCAGCTAACCCGGGCTCTTAAACATCTATTTGCTAGAAATGAAAATTTGATTGTTGATCCCAGTTTAAACCATATTCATTCATTGAGAAAGAAGCTAGTCATGTTGCAGTTAGCTCTTGTAACTGTAGGAGAGAAAGAACTTGAAGAAAAGCTTGGGGAGTACATAGACCCAGTTAAGCTAATGGCCCATTTCTGGGCGTTCATTGCTCATTAG

Coding sequence (CDS)

ATGGTAGCCAACGCCGAGCTCACGGAATATGGTCATGTTCGAGTTACCATGGTTATTCGTCCCTATGGCTCCGCCATTTTCGAAGCAGATCAGGGTTATTTGAATGTTAAGTTCAAGGAGACCAATCACAGGAAGGAACATATACTTTCCACAGACAAGCATTGCAGTGAAGACTATGATATGTGGTTAGTTATAGTTGACGATGATTCCTTTCAGCTAACCCGGGCTCTTAAACATCTATTTGCTAGAAATGAAAATTTGATTGTTGATCCCAGTTTAAACCATATTCATTCATTGAGAAAGAAGCTAGTCATGTTGCAGTTAGCTCTTGTAACTGTAGGAGAGAAAGAACTTGAAGAAAAGCTTGGGGAGTACATAGACCCAGTTAAGCTAATGGCCCATTTCTGGGCGTTCATTGCTCATTAG

Protein sequence

MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNHRKEHILSTDKHCSEDYDMWLVIVDDDSFQLTRALKHLFARNENLIVDPSLNHIHSLRKKLVMLQLALVTVGEKELEEKLGEYIDPVKLMAHFWAFIAH

Homology

[Close]

BLAST of Lsi07G002540 vs. ExPASy Swiss-Prot
Match: Q9FYR6 (Proline--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA6 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 5.6e-10
Identity = 31/43 (72.09%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 1   MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNH 44
           ++A+AEL +YG VR TMVIRPYG AI+EA Q YLNVKFKET H
Sbjct: 80  VIASAELADYGPVRGTMVIRPYGYAIWEAIQDYLNVKFKETGH 122

[Close]

BLAST of Lsi07G002540 vs. ExPASy Swiss-Prot
Match: A0Q2I3 (Proline--tRNA ligase OS=Clostridium novyi (strain NT) OX=386415 GN=proS PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 6.4e-06
Identity = 24/48 (50.00%), Postives = 32/48 (66.67%), Query Frame = 0


Query: 1  MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNHRKEHI 49
          +V  AEL +Y  V+  M+IRPYG AI+E  Q YL+ KFKET H   ++
Sbjct: 25 IVKKAELVDYASVKGCMIIRPYGYAIWENIQKYLDTKFKETGHENVYM 72

[Close]

BLAST of Lsi07G002540 vs. ExPASy Swiss-Prot
Match: Q1AX25 (Proline--tRNA ligase OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=proS PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 8.4e-06
Identity = 35/117 (29.91%), Postives = 59/117 (50.43%), Query Frame = 0


Query: 1   MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNHRKEH--ILSTDKHCSED 60
           +V  A+L +YG VR T  IRPYG AI+E  Q  L+ +FK T H   +  +L  + + +++
Sbjct: 22  LVRMAKLADYGPVRGTFAIRPYGFAIWERIQADLDARFKATGHVNAYFPLLIPESYLTKE 81

Query: 61  YD---------MWLVIVDDDSFQLTRALKHLFARNENLIVDPSLNHIHSLRKKLVML 107
            +          W+ +  DD  +   A++     +E++I D     IHS R   V++
Sbjct: 82  AEHVEGFAPECAWVTVGGDDELEERLAIR---PTSESIICDFYRKWIHSYRDLPVLI 135

[Close]

BLAST of Lsi07G002540 vs. ExPASy Swiss-Prot
Match: Q8ESQ5 (Proline--tRNA ligase OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831) OX=221109 GN=proS PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 2.4e-05
Identity = 36/111 (32.43%), Postives = 54/111 (48.65%), Query Frame = 0


Query: 1   MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNHRK--------EHILSTD 60
           +V  A+L +YG VR TM+I+PYG AI+E  +  L+  FKET H          E +L  +
Sbjct: 26  VVKQADLVDYGQVRGTMIIKPYGYAIWENIKNELDRMFKETGHTNVAFPLFIPESLLQKE 85

Query: 61  KHCSEDYD---MWLVIVDDDSFQLTRALKHLFARNENLIVDPSLNHIHSLR 101
           K   E +     W+    ++      A++     +E L  D   N+IHS R
Sbjct: 86  KDHVEGFAPEVAWVTHGGEEELTERIAVR---PTSEVLFCDYYSNNIHSYR 133

[Close]

BLAST of Lsi07G002540 vs. ExPASy Swiss-Prot
Match: Q72GF9 (Proline--tRNA ligase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=proS PE=3 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 5.4e-05
Identity = 23/47 (48.94%), Postives = 32/47 (68.09%), Query Frame = 0


Query: 1  MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNHRKEH 48
          ++  AEL +YG VR T+V+RPYG AI+E  Q  L+  FKET H+  +
Sbjct: 21 VIQKAELADYGPVRGTIVVRPYGYAIWENIQQVLDRMFKETGHQNAY 67

[Close]

BLAST of Lsi07G002540 vs. ExPASy TrEMBL
Match: A0A6J1C8A3 (Prolyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111008350 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 4.2e-08
Identity = 32/43 (74.42%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 1   MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNH 44
           ++ANAEL +YG VR TMVIRPYG AI+EA Q YLNVKFKET H
Sbjct: 89  VIANAELADYGPVRGTMVIRPYGYAIWEAIQAYLNVKFKETGH 131

[Close]

BLAST of Lsi07G002540 vs. ExPASy TrEMBL
Match: G7J5P9 (Prolyl-tRNA synthetase OS=Medicago truncatula OX=3880 GN=11420887 PE=3 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 5.5e-08
Identity = 32/43 (74.42%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 1   MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNH 44
           ++ANAEL +YG VR TMVIRPYG AI+EA Q YLNVKFKET H
Sbjct: 79  IIANAELADYGPVRGTMVIRPYGYAIWEAIQDYLNVKFKETGH 121

[Close]

BLAST of Lsi07G002540 vs. ExPASy TrEMBL
Match: A0A6A3CZI1 (Prolyl-tRNA synthetase OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00000916pilonHSYRG00247 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 5.5e-08
Identity = 32/43 (74.42%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 1   MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNH 44
           ++ANAEL +YG VR TMVIRPYG AI+EA Q YLNVKFKET H
Sbjct: 88  IIANAELADYGPVRGTMVIRPYGYAIWEAIQDYLNVKFKETGH 130

[Close]

BLAST of Lsi07G002540 vs. ExPASy TrEMBL
Match: A0A6J1B9H7 (Prolyl-tRNA synthetase OS=Herrania umbratica OX=108875 GN=LOC110425282 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 5.5e-08
Identity = 32/43 (74.42%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 1   MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNH 44
           ++ANAEL +YG VR TMVIRPYG AI+EA Q YLNVKFKET H
Sbjct: 119 VIANAELADYGPVRGTMVIRPYGYAIWEAIQDYLNVKFKETGH 161

[Close]

BLAST of Lsi07G002540 vs. ExPASy TrEMBL
Match: B9RIU3 (Prolyl-tRNA synthetase OS=Ricinus communis OX=3988 GN=RCOM_1582810 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 5.5e-08
Identity = 32/43 (74.42%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 1   MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNH 44
           ++ANAEL +YG VR TMVIRPYG AI+EA Q YLNVKFKET H
Sbjct: 85  VIANAELADYGPVRGTMVIRPYGYAIWEAIQDYLNVKFKETGH 127

[Close]

BLAST of Lsi07G002540 vs. NCBI nr
Match: KAF3612616.1 (hypothetical protein FXO38_36752 [Capsicum annuum])

HSP 1 Score: 68.2 bits (165), Expect = 6.6e-08
Identity = 32/44 (72.73%), Postives = 37/44 (84.09%), Query Frame = 0


Query: 1   MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNHR 45
           ++ANAEL +YG VR TMVIRPYG AI+EA Q Y+NVKFKET HR
Sbjct: 86  VIANAELADYGPVRGTMVIRPYGYAIWEAIQEYMNVKFKETGHR 129

[Close]

BLAST of Lsi07G002540 vs. NCBI nr
Match: XP_022136703.1 (proline--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 67.8 bits (164), Expect = 8.6e-08
Identity = 32/43 (74.42%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 1   MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNH 44
           ++ANAEL +YG VR TMVIRPYG AI+EA Q YLNVKFKET H
Sbjct: 89  VIANAELADYGPVRGTMVIRPYGYAIWEAIQAYLNVKFKETGH 131

[Close]

BLAST of Lsi07G002540 vs. NCBI nr
Match: XP_021892106.1 (proline--tRNA ligase, chloroplastic/mitochondrial [Carica papaya])

HSP 1 Score: 67.4 bits (163), Expect = 1.1e-07
Identity = 32/43 (74.42%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 1   MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNH 44
           ++ANAEL +YG VR TMVIRPYG AI+EA Q YLNVKFKET H
Sbjct: 81  VIANAELADYGPVRGTMVIRPYGYAIWEAIQDYLNVKFKETGH 123

[Close]

BLAST of Lsi07G002540 vs. NCBI nr
Match: XP_024172424.1 (proline--tRNA ligase, chloroplastic/mitochondrial [Rosa chinensis] >PRQ18103.1 putative proline--tRNA ligase [Rosa chinensis])

HSP 1 Score: 67.4 bits (163), Expect = 1.1e-07
Identity = 32/43 (74.42%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 1   MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNH 44
           ++ANAEL +YG VR TMVIRPYG AI+EA Q YLNVKFKET H
Sbjct: 81  VIANAELADYGPVRGTMVIRPYGYAIWEAIQDYLNVKFKETGH 123

[Close]

BLAST of Lsi07G002540 vs. NCBI nr
Match: KAG6425666.1 (hypothetical protein SASPL_116111 [Salvia splendens])

HSP 1 Score: 67.4 bits (163), Expect = 1.1e-07
Identity = 32/43 (74.42%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 1   MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNH 44
           ++ANAEL +YG VR TMVIRPYG AI+EA Q YLNVKFKET H
Sbjct: 350 IIANAELADYGPVRGTMVIRPYGYAIWEAIQDYLNVKFKETGH 392

[Close]

BLAST of Lsi07G002540 vs. TAIR 10
Match: AT5G52520.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 4.0e-11
Identity = 31/43 (72.09%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 1   MVANAELTEYGHVRVTMVIRPYGSAIFEADQGYLNVKFKETNH 44
           ++A+AEL +YG VR TMVIRPYG AI+EA Q YLNVKFKET H
Sbjct: 80  VIASAELADYGPVRGTMVIRPYGYAIWEAIQDYLNVKFKETGH 122

The following BLAST results are available for this feature:

BLAST of Lsi07G002540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYR6	5.6e-10	72.09	Proline--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=370...	[more]
A0Q2I3	6.4e-06	50.00	Proline--tRNA ligase OS=Clostridium novyi (strain NT) OX=386415 GN=proS PE=3 SV=...	[more]
Q1AX25	8.4e-06	29.91	Proline--tRNA ligase OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 /...	[more]
Q8ESQ5	2.4e-05	32.43	Proline--tRNA ligase OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 ...	[more]
Q72GF9	5.4e-05	48.94	Proline--tRNA ligase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / H...	[more]

BLAST of Lsi07G002540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C8A3	4.2e-08	74.42	Prolyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111008350 PE=3 SV=1	[more]
G7J5P9	5.5e-08	74.42	Prolyl-tRNA synthetase OS=Medicago truncatula OX=3880 GN=11420887 PE=3 SV=2	[more]
A0A6A3CZI1	5.5e-08	74.42	Prolyl-tRNA synthetase OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00000916pilonH...	[more]
A0A6J1B9H7	5.5e-08	74.42	Prolyl-tRNA synthetase OS=Herrania umbratica OX=108875 GN=LOC110425282 PE=3 SV=1	[more]
B9RIU3	5.5e-08	74.42	Prolyl-tRNA synthetase OS=Ricinus communis OX=3988 GN=RCOM_1582810 PE=3 SV=1	[more]

BLAST of Lsi07G002540 vs. NCBI nr


Match Name	E-value	Identity	Description
KAF3612616.1	6.6e-08	72.73	hypothetical protein FXO38_36752 [Capsicum annuum]	[more]
XP_022136703.1	8.6e-08	74.42	proline--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia]	[more]
XP_021892106.1	1.1e-07	74.42	proline--tRNA ligase, chloroplastic/mitochondrial [Carica papaya]	[more]
XP_024172424.1	1.1e-07	74.42	proline--tRNA ligase, chloroplastic/mitochondrial [Rosa chinensis] >PRQ18103.1 p...	[more]
KAG6425666.1	1.1e-07	74.42	hypothetical protein SASPL_116111 [Salvia splendens]	[more]

BLAST of Lsi07G002540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52520.1	4.0e-11	72.09	Class II aaRS and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 1..106 e-value: 4.9E-6 score: 28.3

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi07G002540.1	Lsi07G002540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009553	embryo sac development
biological_process	GO:0048481	plant ovule development
biological_process	GO:0006433	prolyl-tRNA aminoacylation
biological_process	GO:0010109	regulation of photosynthesis
biological_process	GO:0048316	seed development
cellular_component	GO:0017101	aminoacyl-tRNA synthetase multienzyme complex
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004827	proline-tRNA ligase activity