Lsi07G002520 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi07G002520 vs. ExPASy Swiss-Prot
Match: P9WNH2 (Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2788 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.9e-08
Identity = 36/96 (37.50%), Postives = 53/96 (55.21%), Query Frame = 0

Query: 94  PVVILIHGFAA-----EGIVGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIGL 153
           P ++LIHG         G+   L+++++V  PDLL  G S   +AD S A  A  +   L
Sbjct: 38  PAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDLL 97

Query: 154 MKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVV 185
             L IE+ T+VG S GG VA + A   P+LVD +++
Sbjct: 98  SVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLIL 133

BLAST of Lsi07G002520 vs. ExPASy Swiss-Prot
Match: P9WNH3 (Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2715 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.9e-08
Identity = 36/96 (37.50%), Postives = 53/96 (55.21%), Query Frame = 0

Query: 94  PVVILIHGFAA-----EGIVGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIGL 153
           P ++LIHG         G+   L+++++V  PDLL  G S   +AD S A  A  +   L
Sbjct: 38  PAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDLL 97

Query: 154 MKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVV 185
             L IE+ T+VG S GG VA + A   P+LVD +++
Sbjct: 98  SVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLIL 133

BLAST of Lsi07G002520 vs. ExPASy Swiss-Prot
Match: P0A573 (Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB2734 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.9e-08
Identity = 36/96 (37.50%), Postives = 53/96 (55.21%), Query Frame = 0

Query: 94  PVVILIHGFAA-----EGIVGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIGL 153
           P ++LIHG         G+   L+++++V  PDLL  G S   +AD S A  A  +   L
Sbjct: 38  PAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDLL 97

Query: 154 MKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVV 185
             L IE+ T+VG S GG VA + A   P+LVD +++
Sbjct: 98  SVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLIL 133

BLAST of Lsi07G002520 vs. ExPASy Swiss-Prot
Match: A4ST17 (Pimeloyl-[acyl-carrier protein] methyl ester esterase OS=Aeromonas salmonicida (strain A449) OX=382245 GN=bioH PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 3.0e-05
Identity = 33/100 (33.00%), Postives = 57/100 (57.00%), Query Frame = 0

Query: 94  PVVILIHGFAA-----EGIVGALSKQYSVYVPDLLFFGGS-ITDKADRSPAFQAECLAIG 153
           P ++L+HG+        GI   L+  Y +++ DL  FG S + + +D S  + AE +AI 
Sbjct: 12  PDLVLLHGWGMNGAVWHGIAQQLAAHYRLHLVDLPGFGNSPLREGSDYSLPWLAEQIAIV 71

Query: 154 LMKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGS 188
           L     +KC ++G+S GG+VA +LA  +PE + +++   S
Sbjct: 72  LP----QKCHLLGWSLGGLVASQLALTQPERLHSLITVAS 107

BLAST of Lsi07G002520 vs. ExPASy Swiss-Prot
Match: Q6IE26 (Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 8.7e-05
Identity = 29/101 (28.71%), Postives = 51/101 (50.50%), Query Frame = 0

Query: 93  KPVVILIHGF-----AAEGIVGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIG 152
           KP+++L+HGF     +    +     +Y V   DL  +G S  D      +++ +CL   
Sbjct: 91  KPLMLLLHGFPEFWYSWRHQLREFKSEYRVVALDLRGYGES--DAPAHQESYKLDCLIAD 150

Query: 153 ----LMKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVV 185
               L  LG  KC ++G  +GGM+A+ +A   PE++  ++V
Sbjct: 151 IKDILDSLGYSKCVLIGHDWGGMIAWLIAVCYPEMIMKLIV 189

BLAST of Lsi07G002520 vs. ExPASy TrEMBL
Match: A0A0A0KUS2 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G046660 PE=4 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 1.1e-143
Identity = 262/305 (85.90%), Postives = 276/305 (90.49%), Query Frame = 0

Query: 34  MVNLVAAQRPLLHALMKRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQAPKPPQKPTK 93
           MVNLVAAQRPLLHALMK AGVVPYS+DI+PG +MNFW+P+E+LP+TKKGQ PKPPQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK 60

Query: 94  PVVILIHGFAAEGI------VGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIG 153
           PVVILIHGFAAEGI      VGALSKQYSVYVPDLLFFG SITDK +RSPAFQAECLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 120

Query: 154 LMKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFAS 213
           L KLGIEKC+VVGFSYGGMVAFKL ELRPELVDAMVVSGSILAMTDSIS ETL RLGF S
Sbjct: 121 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS 180

Query: 214 SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 273
           SS+LLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGL+ISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT 240

Query: 274 TLETANDSSSISLQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLERP 333
            +           QKIHLLWGENDQIF V+LARKLQ+ELGDNATFRGIPKAGHLVHLERP
Sbjct: 241 IVPKFT-------QKIHLLWGENDQIFNVDLARKLQQELGDNATFRGIPKAGHLVHLERP 298

BLAST of Lsi07G002520 vs. ExPASy TrEMBL
Match: A0A5A7TNT7 (Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00850 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 7.1e-143
Identity = 261/306 (85.29%), Postives = 279/306 (91.18%), Query Frame = 0

Query: 34  MVNLVAAQRPLLHALMKRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQAPKPPQKPTK 93
           MVNLVAAQRPLLHALMK AGV+PYS+DI+PG +MNFW+P++TLP+TKK Q PK PQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVIPYSVDIEPGTIMNFWVPIDTLPKTKKDQPPKTPQKPTK 60

Query: 94  PVVILIHGFAAEGI------VGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIG 153
           PVVILIHGFAAEGI      VGALSKQYSVYVPDLLFFG SITDK +RSPAFQA+CLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAQCLAIG 120

Query: 154 LMKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFAS 213
           L KLGIE+C+VVGFSYGGMVAFKL EL+PELVDAMVVSGSILAMTDSIS +TLQRLGF S
Sbjct: 121 LRKLGIERCSVVGFSYGGMVAFKLVELQPELVDAMVVSGSILAMTDSISDDTLQRLGFKS 180

Query: 214 SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 273
           SS+LLLPTSVKGLKALLSVAAH+KLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHRKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 240

Query: 274 TLETANDSSSIS-LQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLER 333
           T        S+S LQKIHLLWGENDQIF VELARKLQ+ELGDNATFRGIPKAGHLVHLER
Sbjct: 241 TTALMIFHLSLSPLQKIHLLWGENDQIFNVELARKLQQELGDNATFRGIPKAGHLVHLER 300

BLAST of Lsi07G002520 vs. ExPASy TrEMBL
Match: A0A5D3D2C9 (Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G005000 PE=4 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 7.9e-142
Identity = 258/305 (84.59%), Postives = 276/305 (90.49%), Query Frame = 0

Query: 34  MVNLVAAQRPLLHALMKRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQAPKPPQKPTK 93
           MVNLVAAQRPLLHALMK AGV+PYS+DI+PG +MNFW+P++TLP+TKK Q PK PQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVIPYSVDIEPGTIMNFWVPIDTLPKTKKDQPPKTPQKPTK 60

Query: 94  PVVILIHGFAAEGI------VGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIG 153
           PVVILIHGFAAEGI      VGALSKQYSVYVPDLLFFG SITDK +RSPAFQA+CLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAQCLAIG 120

Query: 154 LMKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFAS 213
           L KLGIE+C+VVGFSYGGMVAFKL EL+PELVDAMVVSGSILAMTDSIS +TLQRLGF S
Sbjct: 121 LRKLGIERCSVVGFSYGGMVAFKLVELQPELVDAMVVSGSILAMTDSISDDTLQRLGFKS 180

Query: 214 SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 273
           SS+LLLPTSVKGLKALLSVAAH+KLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHRKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 240

Query: 274 TLETANDSSSISLQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLERP 333
           T+           QKIHLLWGENDQIF VELARKLQ+ELGDNATFRGIPKAGHLVHLERP
Sbjct: 241 TVPKFT-------QKIHLLWGENDQIFNVELARKLQQELGDNATFRGIPKAGHLVHLERP 298

BLAST of Lsi07G002520 vs. ExPASy TrEMBL
Match: A0A1S3BTU7 (putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493198 PE=4 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 7.9e-142
Identity = 258/305 (84.59%), Postives = 276/305 (90.49%), Query Frame = 0

Query: 34  MVNLVAAQRPLLHALMKRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQAPKPPQKPTK 93
           MVNLVAAQRPLLHALMK AGV+PYS+DI+PG +MNFW+P++TLP+TKK Q PK PQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVIPYSVDIEPGTIMNFWVPIDTLPKTKKDQPPKTPQKPTK 60

Query: 94  PVVILIHGFAAEGI------VGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIG 153
           PVVILIHGFAAEGI      VGALSKQYSVYVPDLLFFG SITDK +RSPAFQA+CLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAQCLAIG 120

Query: 154 LMKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFAS 213
           L KLGIE+C+VVGFSYGGMVAFKL EL+PELVDAMVVSGSILAMTDSIS +TLQRLGF S
Sbjct: 121 LRKLGIERCSVVGFSYGGMVAFKLVELQPELVDAMVVSGSILAMTDSISDDTLQRLGFKS 180

Query: 214 SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 273
           SS+LLLPTSVKGLKALLSVAAH+KLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHRKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 240

Query: 274 TLETANDSSSISLQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLERP 333
           T+           QKIHLLWGENDQIF VELARKLQ+ELGDNATFRGIPKAGHLVHLERP
Sbjct: 241 TVPKFT-------QKIHLLWGENDQIFNVELARKLQQELGDNATFRGIPKAGHLVHLERP 298

BLAST of Lsi07G002520 vs. ExPASy TrEMBL
Match: A0A6J1C537 (monoacylglycerol lipase abhd6-A-like OS=Momordica charantia OX=3673 GN=LOC111008351 PE=4 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 1.3e-139
Identity = 256/305 (83.93%), Postives = 272/305 (89.18%), Query Frame = 0

Query: 34  MVNLVAAQRPLLHALMKRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQAPKPPQKPTK 93
           MVNLVAAQRPLLH LMK AG+VPY ++I+PG VMNFW+P+ETLP+ KKG  PKPPQKPTK
Sbjct: 1   MVNLVAAQRPLLHRLMKMAGIVPYVIEIEPGTVMNFWVPVETLPKCKKGHEPKPPQKPTK 60

Query: 94  PVVILIHGFAAEGI------VGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIG 153
           PVV+L+HGFAAEGI      VGALSKQYSVYVPDLLFFGGS+TDKADRSPAFQAECLA G
Sbjct: 61  PVVVLVHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGGSVTDKADRSPAFQAECLAAG 120

Query: 154 LMKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFAS 213
           L KLGIEKCTVVGFSYGGMVAFKLAEL PELV +MVVSGSILAMTDSISGETL RLGFAS
Sbjct: 121 LGKLGIEKCTVVGFSYGGMVAFKLAELCPELVVSMVVSGSILAMTDSISGETLSRLGFAS 180

Query: 214 SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 273
           SSELLLP SV GLKALLSVAA+KKLWFPDRLHKD+LEVMFNNRKDRAELL+GLIISNKD+
Sbjct: 181 SSELLLPRSVTGLKALLSVAAYKKLWFPDRLHKDYLEVMFNNRKDRAELLEGLIISNKDS 240

Query: 274 TLETANDSSSISLQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLERP 333
           T+           QKIHLLWGENDQIFKVELARKLQ EL DNATFRGIPKAGHLVHLERP
Sbjct: 241 TVPKFT-------QKIHLLWGENDQIFKVELARKLQRELSDNATFRGIPKAGHLVHLERP 298

BLAST of Lsi07G002520 vs. NCBI nr
Match: XP_038904799.1 (monoacylglycerol lipase ABHD6-like [Benincasa hispida])

HSP 1 Score: 524.2 bits (1349), Expect = 9.2e-145
Identity = 266/305 (87.21%), Postives = 277/305 (90.82%), Query Frame = 0

Query: 34  MVNLVAAQRPLLHALMKRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQAPKPPQKPTK 93
           MVNLVAAQRPLLHALMK AGVVP+S+DI+PG VMNFW+PLE LP TKK Q PKPP+KP K
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVVPFSVDIEPGTVMNFWVPLEALPTTKKDQPPKPPKKPKK 60

Query: 94  PVVILIHGFAAEGI------VGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIG 153
           PVV+LIHGFAAEGI      VGALSKQYSVYVPDL+FFGGS+TDKA+RSPAFQAECLA G
Sbjct: 61  PVVVLIHGFAAEGIVTWQFQVGALSKQYSVYVPDLIFFGGSVTDKAERSPAFQAECLATG 120

Query: 154 LMKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFAS 213
           L KLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGF S
Sbjct: 121 LRKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFES 180

Query: 214 SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 273
           SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT
Sbjct: 181 SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 240

Query: 274 TLETANDSSSISLQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLERP 333
            +           QKIHLLWGENDQIFKVELA+KLQEELGDNATFRGIPKAGHLVHLERP
Sbjct: 241 VVPKFT-------QKIHLLWGENDQIFKVELAQKLQEELGDNATFRGIPKAGHLVHLERP 298

BLAST of Lsi07G002520 vs. NCBI nr
Match: XP_004146500.2 (uncharacterized protein LOC101205115 [Cucumis sativus] >KGN53303.1 hypothetical protein Csa_014480 [Cucumis sativus])

HSP 1 Score: 519.6 bits (1337), Expect = 2.3e-143
Identity = 262/305 (85.90%), Postives = 276/305 (90.49%), Query Frame = 0

Query: 34  MVNLVAAQRPLLHALMKRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQAPKPPQKPTK 93
           MVNLVAAQRPLLHALMK AGVVPYS+DI+PG +MNFW+P+E+LP+TKKGQ PKPPQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK 60

Query: 94  PVVILIHGFAAEGI------VGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIG 153
           PVVILIHGFAAEGI      VGALSKQYSVYVPDLLFFG SITDK +RSPAFQAECLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 120

Query: 154 LMKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFAS 213
           L KLGIEKC+VVGFSYGGMVAFKL ELRPELVDAMVVSGSILAMTDSIS ETL RLGF S
Sbjct: 121 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS 180

Query: 214 SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 273
           SS+LLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGL+ISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT 240

Query: 274 TLETANDSSSISLQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLERP 333
            +           QKIHLLWGENDQIF V+LARKLQ+ELGDNATFRGIPKAGHLVHLERP
Sbjct: 241 IVPKFT-------QKIHLLWGENDQIFNVDLARKLQQELGDNATFRGIPKAGHLVHLERP 298

BLAST of Lsi07G002520 vs. NCBI nr
Match: KAA0044794.1 (putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 516.9 bits (1330), Expect = 1.5e-142
Identity = 261/306 (85.29%), Postives = 279/306 (91.18%), Query Frame = 0

Query: 34  MVNLVAAQRPLLHALMKRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQAPKPPQKPTK 93
           MVNLVAAQRPLLHALMK AGV+PYS+DI+PG +MNFW+P++TLP+TKK Q PK PQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVIPYSVDIEPGTIMNFWVPIDTLPKTKKDQPPKTPQKPTK 60

Query: 94  PVVILIHGFAAEGI------VGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIG 153
           PVVILIHGFAAEGI      VGALSKQYSVYVPDLLFFG SITDK +RSPAFQA+CLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAQCLAIG 120

Query: 154 LMKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFAS 213
           L KLGIE+C+VVGFSYGGMVAFKL EL+PELVDAMVVSGSILAMTDSIS +TLQRLGF S
Sbjct: 121 LRKLGIERCSVVGFSYGGMVAFKLVELQPELVDAMVVSGSILAMTDSISDDTLQRLGFKS 180

Query: 214 SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 273
           SS+LLLPTSVKGLKALLSVAAH+KLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHRKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 240

Query: 274 TLETANDSSSIS-LQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLER 333
           T        S+S LQKIHLLWGENDQIF VELARKLQ+ELGDNATFRGIPKAGHLVHLER
Sbjct: 241 TTALMIFHLSLSPLQKIHLLWGENDQIFNVELARKLQQELGDNATFRGIPKAGHLVHLER 300

BLAST of Lsi07G002520 vs. NCBI nr
Match: XP_008452085.1 (PREDICTED: putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform X1 [Cucumis melo] >TYK16669.1 putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 513.5 bits (1321), Expect = 1.6e-141
Identity = 258/305 (84.59%), Postives = 276/305 (90.49%), Query Frame = 0

Query: 34  MVNLVAAQRPLLHALMKRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQAPKPPQKPTK 93
           MVNLVAAQRPLLHALMK AGV+PYS+DI+PG +MNFW+P++TLP+TKK Q PK PQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVIPYSVDIEPGTIMNFWVPIDTLPKTKKDQPPKTPQKPTK 60

Query: 94  PVVILIHGFAAEGI------VGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIG 153
           PVVILIHGFAAEGI      VGALSKQYSVYVPDLLFFG SITDK +RSPAFQA+CLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAQCLAIG 120

Query: 154 LMKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFAS 213
           L KLGIE+C+VVGFSYGGMVAFKL EL+PELVDAMVVSGSILAMTDSIS +TLQRLGF S
Sbjct: 121 LRKLGIERCSVVGFSYGGMVAFKLVELQPELVDAMVVSGSILAMTDSISDDTLQRLGFKS 180

Query: 214 SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 273
           SS+LLLPTSVKGLKALLSVAAH+KLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHRKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 240

Query: 274 TLETANDSSSISLQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLERP 333
           T+           QKIHLLWGENDQIF VELARKLQ+ELGDNATFRGIPKAGHLVHLERP
Sbjct: 241 TVPKFT-------QKIHLLWGENDQIFNVELARKLQQELGDNATFRGIPKAGHLVHLERP 298

BLAST of Lsi07G002520 vs. NCBI nr
Match: XP_022136704.1 (monoacylglycerol lipase abhd6-A-like [Momordica charantia])

HSP 1 Score: 506.1 bits (1302), Expect = 2.6e-139
Identity = 256/305 (83.93%), Postives = 272/305 (89.18%), Query Frame = 0

Query: 34  MVNLVAAQRPLLHALMKRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQAPKPPQKPTK 93
           MVNLVAAQRPLLH LMK AG+VPY ++I+PG VMNFW+P+ETLP+ KKG  PKPPQKPTK
Sbjct: 1   MVNLVAAQRPLLHRLMKMAGIVPYVIEIEPGTVMNFWVPVETLPKCKKGHEPKPPQKPTK 60

Query: 94  PVVILIHGFAAEGI------VGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIG 153
           PVV+L+HGFAAEGI      VGALSKQYSVYVPDLLFFGGS+TDKADRSPAFQAECLA G
Sbjct: 61  PVVVLVHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGGSVTDKADRSPAFQAECLAAG 120

Query: 154 LMKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFAS 213
           L KLGIEKCTVVGFSYGGMVAFKLAEL PELV +MVVSGSILAMTDSISGETL RLGFAS
Sbjct: 121 LGKLGIEKCTVVGFSYGGMVAFKLAELCPELVVSMVVSGSILAMTDSISGETLSRLGFAS 180

Query: 214 SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 273
           SSELLLP SV GLKALLSVAA+KKLWFPDRLHKD+LEVMFNNRKDRAELL+GLIISNKD+
Sbjct: 181 SSELLLPRSVTGLKALLSVAAYKKLWFPDRLHKDYLEVMFNNRKDRAELLEGLIISNKDS 240

Query: 274 TLETANDSSSISLQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLERP 333
           T+           QKIHLLWGENDQIFKVELARKLQ EL DNATFRGIPKAGHLVHLERP
Sbjct: 241 TVPKFT-------QKIHLLWGENDQIFKVELARKLQRELSDNATFRGIPKAGHLVHLERP 298

BLAST of Lsi07G002520 vs. TAIR 10
Match: AT2G18360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 395.6 bits (1015), Expect = 4.6e-110
Identity = 199/307 (64.82%), Postives = 237/307 (77.20%), Query Frame = 0

Query: 34  MVNLVAAQRPLLHALMKRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQA--PKPPQKP 93
           MVN V  Q+PLL+ LMK AGV+PY+++++PG  MNFWIP ETL +TKK        PQKP
Sbjct: 1   MVNFVDLQKPLLYRLMKLAGVIPYTVELEPGTKMNFWIPKETLKKTKKSDKNFAVEPQKP 60

Query: 94  TKPVVILIHGFAAEGI------VGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLA 153
           TKPV++ IHGFAAEGI      VG+L+K+YSVY+PDLLFFGGS +D ADRSPAFQA CL 
Sbjct: 61  TKPVLLFIHGFAAEGIVTWQFQVGSLAKKYSVYIPDLLFFGGSYSDNADRSPAFQAHCLV 120

Query: 154 IGLMKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGF 213
             L  LGIEK T+VGFSYGGMVAFK+AE  PE+V AMVVSGSILAMTD+IS   L +LGF
Sbjct: 121 KSLRILGIEKFTLVGFSYGGMVAFKIAEEYPEMVQAMVVSGSILAMTDTISESNLNQLGF 180

Query: 214 ASSSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNK 273
            SS++LLLPTSVKGLK L ++A HK +WFP RL KDF+EVM  NRK+RAELL+ L+ISNK
Sbjct: 181 KSSADLLLPTSVKGLKTLFTLAVHKPMWFPKRLFKDFIEVMITNRKERAELLEALVISNK 240

Query: 274 DTTLETANDSSSISLQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLE 333
           D T+           QKIHLLWGE+DQIF +E A+ ++E+LG+NAT   I KAGHL HLE
Sbjct: 241 DVTIPRFQ-------QKIHLLWGESDQIFNLEFAKSMKEQLGENATMESIKKAGHLAHLE 300

BLAST of Lsi07G002520 vs. TAIR 10
Match: AT4G36610.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 384.4 bits (986), Expect = 1.1e-106
Identity = 190/305 (62.30%), Postives = 234/305 (76.72%), Query Frame = 0

Query: 34  MVNLVAAQRPLLHALMKRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQAPKPPQKPTK 93
           MVN V  Q+PLL+ LMK AGVVPY+L+I+PG  +NFW+P ETL +      P  P KP K
Sbjct: 1   MVNFVEVQKPLLYGLMKMAGVVPYTLEIEPGTKINFWVPKETLKKNSGTGKPTKPDKPKK 60

Query: 94  PVVILIHGFAAEGI------VGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIG 153
           PVV+LIHGFA EGI      VGALSK+YSVY+PDLLFFGGS TD +DRSPAFQA+CL  G
Sbjct: 61  PVVLLIHGFAGEGIVTWQFQVGALSKKYSVYIPDLLFFGGSYTDNSDRSPAFQADCLVKG 120

Query: 154 LMKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFAS 213
           L  LG++K   VGFSYGGMVAFK+AE  P++V A+VVSGSI  MTD+I+  +L RLGF+S
Sbjct: 121 LRILGVDKFVPVGFSYGGMVAFKIAEAYPDMVRAIVVSGSIPTMTDTINEASLNRLGFSS 180

Query: 214 SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 273
           S++LLLPTSV GLKAL ++A HK LWFP RL KD++EVMFNNRK+RAELL+ +++SNK+ 
Sbjct: 181 STDLLLPTSVTGLKALFTIAVHKPLWFPKRLFKDYIEVMFNNRKERAELLEAVVVSNKEA 240

Query: 274 TLETANDSSSISLQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLERP 333
            +           +KIH LWGE+DQIF +ELAR ++E++G+NAT   I KAGHLV LERP
Sbjct: 241 QIPHFP-------RKIHFLWGESDQIFDLELARDMKEQIGENATIESIKKAGHLVQLERP 298

BLAST of Lsi07G002520 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 156.0 bits (393), Expect = 6.1e-38
Identity = 100/297 (33.67%), Postives = 157/297 (52.86%), Query Frame = 0

Query: 44  LLHALMKRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQAPKPPQKPT-KPVVILIHGF 103
           LL      AG+   +L ID    + FW           G  P    + T KP ++L+HGF
Sbjct: 15  LLRRRFSSAGLSLQTLSIDSETTIQFW-----------GPPPSSSSENTQKPSLLLLHGF 74

Query: 104 AAEGI------VGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIGLMKLGIEKC 163
               +      V  LS  + +YVPDL+FFGGS +   +RS  FQA C+   + KL +E+ 
Sbjct: 75  GPSAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEVERF 134

Query: 164 TVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFASSSELLLPTS 223
           +VVG SYGG VA+ +A++ PE V+ +V++ S + +  S +   + R       E++LP S
Sbjct: 135 SVVGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSDNEAFIARAKCHRIKEVMLPAS 194

Query: 224 VKGLKALLSVAAHKKL-WFPDRLHKDFLEVMFN-NRKDRAELLQGLIISNKDTTLETAND 283
              L+    + + K+L + PD +  DF + M++  R+++AELL+GL I   D T      
Sbjct: 195 ATDLRRFSGMVSSKRLDYVPDFVLNDFCQKMYSEKREEKAELLEGLSIGKDDKT------ 254

Query: 284 SSSISLQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLERPCVYN 332
           + S   Q + L+WGE DQ+F +++A  L+E LG  AT + I K  H+   E+   +N
Sbjct: 255 NVSPIQQDVMLIWGEQDQVFPLKMAHDLKEMLGTKATLKVIQKTSHIPQTEKSKEFN 294

BLAST of Lsi07G002520 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 146.4 bits (368), Expect = 4.9e-35
Identity = 88/290 (30.34%), Postives = 157/290 (54.14%), Query Frame = 0

Query: 50  KRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQAPKPPQKPTKPVVILIHGFAAEGI-- 109
           KR+G+ P ++D+  G V+NFW+              KP  KP KP ++LIHG  A  I  
Sbjct: 20  KRSGLRPVTIDLKDGTVVNFWV-----------SKTKPESKP-KPNLLLIHGLGATAIWQ 79

Query: 110 ----VGALSKQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIGLMKLGIEKCTVVGFSY 169
                  LS+ +++Y+PDL+FFGGS T + +RS  FQA+ L   L    ++K ++VG SY
Sbjct: 80  WYDVARRLSRYFNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSVKKFSLVGLSY 139

Query: 170 GGMVAFKLAELRPELVDAMVVSGSILAMTD-SISGETLQRLGFASSSELLLPTSVKGLKA 229
           GG V +++A +  + V+ +V+  + + + +  +     +      +S++L+P SVK L+ 
Sbjct: 140 GGFVGYRMASMYADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILVPESVKKLRE 199

Query: 230 LLSVAAHKKL---WFPDRLHKDFLE--VMFNNRKDRAELLQGLIISNKDTTLETANDSSS 289
           L+    +K       P  L  DF+E  +  +N +++ EL++ +    KD  +        
Sbjct: 200 LMGYIFYKPALARLVPTCLLHDFIEHALTRDNMEEKRELIKAI---PKDRIISEIPKLKQ 259

Query: 290 ISLQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLERP 328
            +L    ++WGE+DQ+F +E+ ++L++ +GDN     I + GH+ + E+P
Sbjct: 260 PTL----IIWGEHDQVFPLEMGKRLEKHVGDNGKLVIIKRTGHIFNFEKP 290

BLAST of Lsi07G002520 vs. TAIR 10
Match: AT4G33180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 146.0 bits (367), Expect = 6.4e-35
Identity = 93/295 (31.53%), Postives = 154/295 (52.20%), Query Frame = 0

Query: 45  LHALMKRAGVVPYSLDIDPGIVMNFWIPLETLPRTKKGQAPKPP----QKPTKPVVILIH 104
           L   ++ AG+   +L ID    ++FW P              PP        +PV++L+H
Sbjct: 17  LRRCLRAAGLTSQTLSIDSETTIHFWGP--------------PPLDHRSDDDRPVMLLLH 76

Query: 105 GFAAEGI------VGALS-KQYSVYVPDLLFFGGSITDKADRSPAFQAECLAIGLMKLGI 164
           GF    +      + A S   + VY PDL+FFG S +   +R+  FQAEC+A  + K+GI
Sbjct: 77  GFGPSSMWQWRRQMQAFSPSAFRVYSPDLVFFGDSTSSSTNRTEVFQAECMAKLMAKIGI 136

Query: 165 EKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFASSSELLL 224
            K  V G SYGG VA+ +A++ PE V+ +V++ S + M        LQR       +++L
Sbjct: 137 GKYNVAGTSYGGFVAYHMAKMWPEKVEKVVIASSGINMRKCDGESLLQRSNCECIEKVML 196

Query: 225 PTSVKGLKALLSVAAHKKL--WFPDRLHKDFLEVMF-NNRKDRAELLQGLIISNKDTTLE 284
           P++    + L+++A+  +L   FPD L  D +  ++  NRK++ ELL+G+     +    
Sbjct: 197 PSTATEFRTLMALASSWRLVRMFPDALWNDVINNLYKKNRKEKIELLKGVTFGRSENL-- 256

Query: 285 TANDSSSISLQKIHLLWGENDQIFKVELARKLQEELGDNATFRGIPKAGHLVHLE 326
              +  S+S Q++ ++WG+ DQIF V++A +L+E LGD      I    H+  +E
Sbjct: 257 ---NIDSLS-QEVLIVWGDKDQIFPVKMAYELKEILGDKTKLEIIDNTSHVPQIE 291