Lsi07G001870 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G001870
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionMitochondrial transcription termination factor family protein
Locationchr07: 2048644 .. 2049630 (+)
RNA-Seq ExpressionLsi07G001870
SyntenyLsi07G001870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCTGCCGTAAAGGCGTTGTATCAACGGAAAAGCCTCAATCCGTTTACAAATATCTTTCAGAGCTCGGATTCTCCGACGCCCACATTCAATCGGCGATTCGACTCGTGCCGCAGATTGCGTTTTCTAGCATTGAAAAAACTCTGAAACCGAAGATTGAGTTCTTCCAGAATCTTGGCTTGGTCGGCTCTGATTTGGGTAAGTTCATTTCCAGGCATTCTTCGCTTTTGACTGTTAGTTTGGAGAAGAAATTGATGCCCAGTGTTGAGATTCTTAAGAATGTTTTCCCCAAGGATAATTGTAATAGAGATCTCCTGTGTGTTATGCGGCGATGTTCTGATATGGTTATGAGATCCCCGTATAAAAGGTTGTCTGTAAATATTAATTACTTACAAAGTTGTGGGATTGTTGATTCTCAACTCTCTATGTTACTGAAGATGCAACCTGCATTTTTTGGTAAGCGTGAATCTCGAGTTAAAGATATTGTTTCCATGGTTGTAGAAACTGGTTTTTCTACCGATACTAAAATGTTTGTTCATGGACTTCAAGCTATCAGTTGTGTAAGTAATGCGACCTTTAACAAGAAAGTGGACCTGATTTGTAGCTTTGGAATAACTGAGAAAGAATGTATGAGAATGTTTACTTCTGCTCCTTTTTTGATAAGGACTTCCATTGATAAACTTAAGCTTGGTCTAGAATTCTTCATGAATGAGGCAAAACTTAGCAAATCACATATTCTTGGTAAACCTACTTGTTTGATGCACTCCATGCAGGGGAGGGTGCTCCCTCGTTATAGAGTTCTACAGGTTGTGAAGTCGAAGAGGCTATTGAAGAAGCAACCCAAATTTATCCATATATTGGGGATGTCTGATCAGGATTTCTTGGATAAATTCGTGTGTAGGTTTCCTGATAATATGAAAGATTTGTTGGTGGCCTTTAGAGGTAATTCTGTGGGTGCATTTCAGCCTAAGGAGTTAACAACATGA

mRNA sequence

ATGAGCTGCCGTAAAGGCGTTGTATCAACGGAAAAGCCTCAATCCGTTTACAAATATCTTTCAGAGCTCGGATTCTCCGACGCCCACATTCAATCGGCGATTCGACTCGTGCCGCAGATTGCGTTTTCTAGCATTGAAAAAACTCTGAAACCGAAGATTGAGTTCTTCCAGAATCTTGGCTTGGTCGGCTCTGATTTGGGTAAGTTCATTTCCAGGCATTCTTCGCTTTTGACTGTTAGTTTGGAGAAGAAATTGATGCCCAGTGTTGAGATTCTTAAGAATGTTTTCCCCAAGGATAATTGTAATAGAGATCTCCTGTGTGTTATGCGGCGATGTTCTGATATGGTTATGAGATCCCCGTATAAAAGGTTGTCTGTAAATATTAATTACTTACAAAGTTGTGGGATTGTTGATTCTCAACTCTCTATGTTACTGAAGATGCAACCTGCATTTTTTGGTAAGCGTGAATCTCGAGTTAAAGATATTGTTTCCATGGTTGTAGAAACTGGTTTTTCTACCGATACTAAAATGTTTGTTCATGGACTTCAAGCTATCAGTTGTGTAAGTAATGCGACCTTTAACAAGAAAGTGGACCTGATTTGTAGCTTTGGAATAACTGAGAAAGAATGTATGAGAATGTTTACTTCTGCTCCTTTTTTGATAAGGACTTCCATTGATAAACTTAAGCTTGGTCTAGAATTCTTCATGAATGAGGCAAAACTTAGCAAATCACATATTCTTGGTAAACCTACTTGTTTGATGCACTCCATGCAGGGGAGGGTGCTCCCTCGTTATAGAGTTCTACAGGTTGTGAAGTCGAAGAGGCTATTGAAGAAGCAACCCAAATTTATCCATATATTGGGGATGTCTGATCAGGATTTCTTGGATAAATTCGTGTGTAGGTTTCCTGATAATATGAAAGATTTGTTGGTGGCCTTTAGAGGTAATTCTGTGGGTGCATTTCAGCCTAAGGAGTTAACAACATGA

Coding sequence (CDS)

ATGAGCTGCCGTAAAGGCGTTGTATCAACGGAAAAGCCTCAATCCGTTTACAAATATCTTTCAGAGCTCGGATTCTCCGACGCCCACATTCAATCGGCGATTCGACTCGTGCCGCAGATTGCGTTTTCTAGCATTGAAAAAACTCTGAAACCGAAGATTGAGTTCTTCCAGAATCTTGGCTTGGTCGGCTCTGATTTGGGTAAGTTCATTTCCAGGCATTCTTCGCTTTTGACTGTTAGTTTGGAGAAGAAATTGATGCCCAGTGTTGAGATTCTTAAGAATGTTTTCCCCAAGGATAATTGTAATAGAGATCTCCTGTGTGTTATGCGGCGATGTTCTGATATGGTTATGAGATCCCCGTATAAAAGGTTGTCTGTAAATATTAATTACTTACAAAGTTGTGGGATTGTTGATTCTCAACTCTCTATGTTACTGAAGATGCAACCTGCATTTTTTGGTAAGCGTGAATCTCGAGTTAAAGATATTGTTTCCATGGTTGTAGAAACTGGTTTTTCTACCGATACTAAAATGTTTGTTCATGGACTTCAAGCTATCAGTTGTGTAAGTAATGCGACCTTTAACAAGAAAGTGGACCTGATTTGTAGCTTTGGAATAACTGAGAAAGAATGTATGAGAATGTTTACTTCTGCTCCTTTTTTGATAAGGACTTCCATTGATAAACTTAAGCTTGGTCTAGAATTCTTCATGAATGAGGCAAAACTTAGCAAATCACATATTCTTGGTAAACCTACTTGTTTGATGCACTCCATGCAGGGGAGGGTGCTCCCTCGTTATAGAGTTCTACAGGTTGTGAAGTCGAAGAGGCTATTGAAGAAGCAACCCAAATTTATCCATATATTGGGGATGTCTGATCAGGATTTCTTGGATAAATTCGTGTGTAGGTTTCCTGATAATATGAAAGATTTGTTGGTGGCCTTTAGAGGTAATTCTGTGGGTGCATTTCAGCCTAAGGAGTTAACAACATGA

Protein sequence

MSCRKGVVSTEKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLGLVGSDLGKFISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSPYKRLSVNINYLQSCGIVDSQLSMLLKMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVHGLQAISCVSNATFNKKVDLICSFGITEKECMRMFTSAPFLIRTSIDKLKLGLEFFMNEAKLSKSHILGKPTCLMHSMQGRVLPRYRVLQVVKSKRLLKKQPKFIHILGMSDQDFLDKFVCRFPDNMKDLLVAFRGNSVGAFQPKELTT
Homology
BLAST of Lsi07G001870 vs. ExPASy Swiss-Prot
Match: F4JVI3 (Transcription termination factor MTERF5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF5 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.5e-08
Identity = 47/165 (28.48%), Postives = 80/165 (48.48%), Query Frame = 0

Query: 12  KPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLGLVGSDLGKFIS 71
           K + V ++L +LG   + I + +   PQI   S+   LKP + F + LG+  +   K IS
Sbjct: 231 KIKPVVEFLLDLGIPKSDIPTILCKRPQICGISLTDNLKPTMAFLETLGIDKNQWAKIIS 290

Query: 72  RHSSLLTVSLEKKLMPSVEILKNV-FPKDNCNRDLLCVMRRCSDMVMRSPYKRLSVNINY 131
           R  ++LT S  +KL  +VE L      ++   R    ++ RC +++  S   +L   + Y
Sbjct: 291 RFPAILTYS-RQKLTSTVEFLSQTGLTEEQIGR----ILTRCPNIMSYSVEDKLRPTMEY 350

Query: 132 LQSCGIVDSQLSMLLKMQPAFFG-KRESRVKDIVSMVVETGFSTD 175
            +S  +    +++LL   P  FG   ES +K +    +E GF  D
Sbjct: 351 FRSLNV---DVAVLLHRCPQTFGLSIESNLKPVTEFFLEKGFGLD 387

BLAST of Lsi07G001870 vs. ExPASy Swiss-Prot
Match: Q9FM80 (Transcription termination factor MTERF9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF9 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.2e-07
Identity = 67/302 (22.19%), Postives = 124/302 (41.06%), Query Frame = 0

Query: 19  YLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLGLVGSDLGKFISRHSSLLT 78
           YL  +G     I+  +   PQI   ++E  LK  I F   LG+  S +G+ ++   SL +
Sbjct: 225 YLLSVGVKHRDIKRMLLRQPQILQYTVENNLKAHISFLMGLGIPNSKIGQIVAATPSLFS 284

Query: 79  VSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSPYKRLSVNINYLQSCGIVD 138
            S+E  L P++  L                                      ++  GI +
Sbjct: 285 YSVENSLRPTIRYL--------------------------------------IEEVGIKE 344

Query: 139 SQLSMLLKMQPAFFGKRESRVKDIVSMVV--ETGFSTDT--KMFVHGLQAISCVSNATFN 198
           + +  ++++ P    +R     +   M +  E G   D+  KM     Q +    +  F 
Sbjct: 345 TDVGKVVQLSPQILVQRLDITWNTRYMFLSKELGAPRDSVVKMVKKHPQLLHYSIDDGFL 404

Query: 199 KKVDLICSFGITEKECMRMFTSAPFLIRTSI-DKLKLGLEFFMNEAKLSKSHILGK-PTC 258
            +++ + S G+   + +++ TS   ++  S+ D LK    + +NE   ++ HIL K P  
Sbjct: 405 PRINFLRSIGMCNSDILKVLTSLTQVLSLSLEDNLKPKYMYLVNELN-NEVHILTKYPMY 464

Query: 259 LMHSMQGRVLPRYRVLQVVKSKRLLKKQPKFIHILGMSDQDFLDKFVCRFPDNMKDLLVA 315
           L  S+  R+ PR+R L  +K    ++K P  +  L  +D+ F  ++         D  +A
Sbjct: 465 LSLSLDQRIRPRHRFLVELKK---VRKGPFPLSSLVPNDESFCQQWA----GTSVDTYLA 480

BLAST of Lsi07G001870 vs. ExPASy Swiss-Prot
Match: F4IHL3 (Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF2 PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 2.6e-05
Identity = 35/149 (23.49%), Postives = 64/149 (42.95%), Query Frame = 0

Query: 6   GVVSTEKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLGLVGSD 65
           G  S +  +    YL E G S   +   +   P +   SIE+  KP +++F  LG+    
Sbjct: 345 GFFSFQVMEKKINYLKEFGLSTEEVGRLLAYKPHLMGCSIEERWKPLVKYFYYLGIPKEG 404

Query: 66  LGKFISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSPYKRL- 125
           + + +     L  + LEK + P V  L+ +   +    ++L    +   ++  S YK++ 
Sbjct: 405 MKRILVVKPILYCIDLEKTIAPKVRFLQEMGIPNEAIGNMLV---KFPSLLTNSLYKKIR 464

Query: 126 SVNINYLQSCGIVDSQLSMLLKMQPAFFG 154
            V I  L   G+    +  ++ M PA  G
Sbjct: 465 PVVIFLLTRAGVTQKDIGKVIAMDPALLG 490

BLAST of Lsi07G001870 vs. ExPASy Swiss-Prot
Match: Q9SZL6 (Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MTERF6 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 2.6e-05
Identity = 48/200 (24.00%), Postives = 89/200 (44.50%), Query Frame = 0

Query: 11  EKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLGL-VGSDLGKF 70
           EK   +  +   LG  +  +   I   P++   SI+  L   + F  +LGL     +GK 
Sbjct: 112 EKLCPLLAFFQALGVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKV 171

Query: 71  ISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSPYKRLSVNIN 130
           + ++  L+  S++K+L P+ E LK+          +  V+     ++ R   K L  N +
Sbjct: 172 LVKNPFLMGYSVDKRLRPTTEFLKSSVGLS--EDGIKSVVMNFPQLLCRDVNKILKPNYD 231

Query: 131 YLQSCGIVDSQLSMLLKMQPAFFGKR-----ESRVKDIVSMVVE--TGFSTDTKMFVHGL 190
           YL+ CG  DSQ++ ++   P    K      + R++ +V ++       ++  + F HGL
Sbjct: 232 YLKECGFGDSQIATMVTGYPQILIKSVKNSLQPRIRFLVQVMGRGMDEVASYPEFFHHGL 291

Query: 191 QAISCVSNATFNKKVDLICS 203
           +     S     KK ++ CS
Sbjct: 292 KK-KVESRFKLVKKNNIDCS 308

BLAST of Lsi07G001870 vs. ExPASy Swiss-Prot
Match: Q84X53 (Transcription termination factor MTEF1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF1 PE=2 SV=2)

HSP 1 Score: 50.1 bits (118), Expect = 5.7e-05
Identity = 36/116 (31.03%), Postives = 59/116 (50.86%), Query Frame = 0

Query: 10  TEKPQS----VYKYLS-ELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLGLVGS 69
           T  P+S    V ++LS E+  S+  I  +I   P++  SS++  L+P + F + LG VG 
Sbjct: 94  TSDPESEILPVLRFLSNEISISEQDIPKSISRCPRLLISSVDYQLRPALTFLKTLGFVGR 153

Query: 70  DLGKFISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSP 121
           D     SR++ LL  ++E+ L+P +E L+           L       + MV+RSP
Sbjct: 154 D--TITSRNTVLLVSNVERTLIPKIEYLE---------EGLGFTREEVAKMVVRSP 198

BLAST of Lsi07G001870 vs. ExPASy TrEMBL
Match: A0A6J1JB80 (uncharacterized protein LOC111482884 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482884 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.2e-143
Identity = 261/328 (79.57%), Postives = 292/328 (89.02%), Query Frame = 0

Query: 1   MSCRKGVVSTEKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLG 60
           MS R+G+ STEKPQSVYKYLSELGFS+AHIQS IRL PQIAFS+IEKTLKPKIEFFQNLG
Sbjct: 64  MSSRRGIASTEKPQSVYKYLSELGFSEAHIQSTIRLAPQIAFSNIEKTLKPKIEFFQNLG 123

Query: 61  LVGSDLGKFISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSP 120
           LVGSDLG+FIS+HS+LLT+SLEK+LMPSVEILK+VFPKD CN DLL V+RRCSDM+MRSP
Sbjct: 124 LVGSDLGRFISKHSTLLTISLEKRLMPSVEILKSVFPKDECNSDLLQVLRRCSDMLMRSP 183

Query: 121 YKRLSVNINYLQSCGIVDSQLSMLLKMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVH 180
           Y RL VNINYLQSCGIV SQLSMLLK QPA FG RES+V+D+VSMVVETGFST+T+MFVH
Sbjct: 184 YTRLPVNINYLQSCGIVGSQLSMLLKRQPALFGMRESQVRDLVSMVVETGFSTNTRMFVH 243

Query: 181 GLQAISCVSNATFNKKVDLICSFGITEKECMRMFTSAPFLIRTSIDKLKLGLEFFMNEAK 240
           GL AIS VSNATF KKV+LICSFGITEKECMRMFTSAP LIRTS+ KLK GLEFFMNEA+
Sbjct: 244 GLHAISSVSNATFKKKVELICSFGITEKECMRMFTSAPVLIRTSVGKLKRGLEFFMNEAE 303

Query: 241 LSKSHILGKPTCLMHSMQGRVLPRYRVLQVVKSKRLLKKQPKFIHILGMSDQDFLDKFVC 300
           +S+S I+ KPTCLMH+MQGRVLPRYRVLQVV SKRL KK  + I ILGMSD++FLDKFV 
Sbjct: 304 VSRSDIVRKPTCLMHAMQGRVLPRYRVLQVVNSKRLSKKHLQLIDILGMSDENFLDKFVH 363

Query: 301 RFPDNMKDLLVAFRGNSVGAFQPKELTT 329
           RFPD++ +LL AFRG  +   QPKEL T
Sbjct: 364 RFPDHVTELLAAFRGQCMDELQPKELET 391

BLAST of Lsi07G001870 vs. ExPASy TrEMBL
Match: A0A6J1EN21 (uncharacterized protein LOC111435860 OS=Cucurbita moschata OX=3662 GN=LOC111435860 PE=4 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 2.1e-143
Identity = 262/328 (79.88%), Postives = 293/328 (89.33%), Query Frame = 0

Query: 1   MSCRKGVVSTEKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLG 60
           M  R+G+ STEKPQSVYKYLSELGFS AHIQS IRL PQIAFS+IE+TLKPKIEFFQNLG
Sbjct: 64  MRSRRGLESTEKPQSVYKYLSELGFSKAHIQSTIRLAPQIAFSNIERTLKPKIEFFQNLG 123

Query: 61  LVGSDLGKFISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSP 120
           LVGSDLG+FIS+HS+LLTVSLEK+LMPSVEILK+VFPKD CN DLL VM+RCSDM+MRSP
Sbjct: 124 LVGSDLGRFISKHSTLLTVSLEKRLMPSVEILKSVFPKDECNSDLLQVMQRCSDMLMRSP 183

Query: 121 YKRLSVNINYLQSCGIVDSQLSMLLKMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVH 180
           Y RL VNI+YLQSCGIV SQLSMLLK QPA FG RES+V+DIVSMVVETGFST+TKMFVH
Sbjct: 184 YTRLLVNISYLQSCGIVGSQLSMLLKRQPALFGMRESQVRDIVSMVVETGFSTNTKMFVH 243

Query: 181 GLQAISCVSNATFNKKVDLICSFGITEKECMRMFTSAPFLIRTSIDKLKLGLEFFMNEAK 240
           GL +IS VSNATF KKV+LICSFGITEKECMRMFTSAP LIRTS+ KLK GLEFFMNEA+
Sbjct: 244 GLHSISSVSNATFKKKVELICSFGITEKECMRMFTSAPGLIRTSVGKLKRGLEFFMNEAE 303

Query: 241 LSKSHILGKPTCLMHSMQGRVLPRYRVLQVVKSKRLLKKQPKFIHILGMSDQDFLDKFVC 300
           +S+S I+ KPTCLMH+MQGRVLPRYRVLQVVKSKRLL+K P+ + ILGMSD+DFLDKFV 
Sbjct: 304 VSRSDIVCKPTCLMHAMQGRVLPRYRVLQVVKSKRLLEKHPQLLDILGMSDEDFLDKFVH 363

Query: 301 RFPDNMKDLLVAFRGNSVGAFQPKELTT 329
           RFPD++ +LL AFRG  +   QPKEL T
Sbjct: 364 RFPDHVTELLAAFRGQCMDELQPKELET 391

BLAST of Lsi07G001870 vs. ExPASy TrEMBL
Match: A0A6J1END2 (uncharacterized protein LOC111436067 OS=Cucurbita moschata OX=3662 GN=LOC111436067 PE=3 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 3.9e-142
Identity = 260/328 (79.27%), Postives = 289/328 (88.11%), Query Frame = 0

Query: 1   MSCRKGVVSTEKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLG 60
           M  R+G+ STEKPQSVYKYLSELGFS AHIQS IRL PQIAFS+IEKTLKPKIEFFQN G
Sbjct: 64  MRSRRGIESTEKPQSVYKYLSELGFSKAHIQSTIRLAPQIAFSNIEKTLKPKIEFFQNFG 123

Query: 61  LVGSDLGKFISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSP 120
           LVGSDLG+FIS+HS+LLTVSLEKKLMPSVEILK+VFPKD CN DLL  M+RC D +MRSP
Sbjct: 124 LVGSDLGRFISKHSTLLTVSLEKKLMPSVEILKSVFPKDECNSDLLQGMQRCCDKLMRSP 183

Query: 121 YKRLSVNINYLQSCGIVDSQLSMLLKMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVH 180
           Y RL VNINYLQSCGIV SQLSMLLK QPA FGKRES+V++IVSMVVETGFST+TKMFVH
Sbjct: 184 YTRLLVNINYLQSCGIVGSQLSMLLKRQPALFGKRESQVREIVSMVVETGFSTNTKMFVH 243

Query: 181 GLQAISCVSNATFNKKVDLICSFGITEKECMRMFTSAPFLIRTSIDKLKLGLEFFMNEAK 240
           GL AIS V+NATF KKV+LICSFGITEKECMRMFTSAP LIRTS+ KLK GLEFFMNEA+
Sbjct: 244 GLHAISSVTNATFKKKVELICSFGITEKECMRMFTSAPVLIRTSVGKLKRGLEFFMNEAE 303

Query: 241 LSKSHILGKPTCLMHSMQGRVLPRYRVLQVVKSKRLLKKQPKFIHILGMSDQDFLDKFVC 300
           +S+S I+ KPTCLMH+MQGRVLPRYRVLQVVKSK LLKK P+ + ILGMSD+DFLDKFV 
Sbjct: 304 VSRSDIVRKPTCLMHAMQGRVLPRYRVLQVVKSKSLLKKHPQLLDILGMSDEDFLDKFVH 363

Query: 301 RFPDNMKDLLVAFRGNSVGAFQPKELTT 329
           RFPD++ +LL AFRG  +   QPK+L T
Sbjct: 364 RFPDHVIELLAAFRGQCMDELQPKQLET 391

BLAST of Lsi07G001870 vs. ExPASy TrEMBL
Match: A0A6J1J5Y4 (uncharacterized protein LOC111482884 isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111482884 PE=3 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 4.3e-141
Identity = 259/324 (79.94%), Postives = 285/324 (87.96%), Query Frame = 0

Query: 1   MSCRKGVVSTEKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLG 60
           MS R+G+ STEKPQSVYKYLSELGFS+AHIQS IRL PQIAFS+IEKTLKPKIEFFQNLG
Sbjct: 64  MSSRRGIASTEKPQSVYKYLSELGFSEAHIQSTIRLAPQIAFSNIEKTLKPKIEFFQNLG 123

Query: 61  LVGSDLGKFISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSP 120
           LVGSDLG FIS+HS+LLT+SLEK+LMPSVEILK+VFPKD CN D L VMRRCSDM+ RSP
Sbjct: 124 LVGSDLGWFISKHSTLLTISLEKRLMPSVEILKSVFPKDECNSDFLQVMRRCSDMLKRSP 183

Query: 121 YKRLSVNINYLQSCGIVDSQLSMLLKMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVH 180
           Y RL VNINYLQSCGIV SQLSMLLK QPA FG RES+V+DIVSMVVETGFST+TKMFVH
Sbjct: 184 YTRLLVNINYLQSCGIVGSQLSMLLKRQPALFGMRESQVRDIVSMVVETGFSTNTKMFVH 243

Query: 181 GLQAISCVSNATFNKKVDLICSFGITEKECMRMFTSAPFLIRTSIDKLKLGLEFFMNEAK 240
           GL AIS VSNATF KKV+LICSFGITEKECMRMFTSAP LIRTS+ KLK GLEFFMNEA+
Sbjct: 244 GLHAISSVSNATFKKKVELICSFGITEKECMRMFTSAPVLIRTSVGKLKRGLEFFMNEAE 303

Query: 241 LSKSHILGKPTCLMHSMQGRVLPRYRVLQVVKSKRLLKKQPKFIHILGMSDQDFLDKFVC 300
           +S+S I+ KPTCLMH+MQGRVLPRYRVLQVV SKRL KK  + I ILGMSD++FLDKFV 
Sbjct: 304 VSRSDIVRKPTCLMHAMQGRVLPRYRVLQVVNSKRLSKKHLQLIDILGMSDENFLDKFVH 363

Query: 301 RFPDNMKDLLVAFRGNSVGAFQPK 325
           RFPD + +LL AFRG  +   QPK
Sbjct: 364 RFPDQVTELLAAFRGQCMDELQPK 387

BLAST of Lsi07G001870 vs. ExPASy TrEMBL
Match: A0A6J1C8W1 (uncharacterized protein LOC111008506 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008506 PE=3 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.6e-138
Identity = 255/326 (78.22%), Postives = 284/326 (87.12%), Query Frame = 0

Query: 1   MSCRKGVVSTEKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLG 60
           MSCRKGV STEKP+SV KYLSELGFSDAHIQSAIR+ PQIAFSS+EKTLKPKIEFFQNLG
Sbjct: 61  MSCRKGVESTEKPRSVCKYLSELGFSDAHIQSAIRISPQIAFSSVEKTLKPKIEFFQNLG 120

Query: 61  LVGSDLGKFISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSP 120
           LVGSDLGKFIS HSSLLTVSL+ KL PSVEILKNVFPKD  N +LL VMRRCSD++MR P
Sbjct: 121 LVGSDLGKFISNHSSLLTVSLKNKLRPSVEILKNVFPKDEDNSNLLQVMRRCSDILMRCP 180

Query: 121 YKRLSVNINYLQSCGIVDSQLSMLLKMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVH 180
             RL VNINY +SCGIVDSQLSMLLK QP  FG+RESRV+D+VSM VETGFST+TKMFVH
Sbjct: 181 ESRLLVNINYFRSCGIVDSQLSMLLKRQPVLFGRRESRVRDLVSMAVETGFSTNTKMFVH 240

Query: 181 GLQAISCVSNATFNKKVDLICSFGITEKECMRMFTSAPFLIRTSIDKLKLGLEFFMNEAK 240
           GL AIS VSNATF KKV+LICSFG TEKECM+MFTSAP LIRTSI KLK G+EFFMNEAK
Sbjct: 241 GLHAISSVSNATFKKKVELICSFGFTEKECMKMFTSAPVLIRTSIAKLKTGIEFFMNEAK 300

Query: 241 LSKSHILGKPTCLMHSMQGRVLPRYRVLQVVKSKRLLKKQPKFIHILGMSDQDFLDKFVC 300
           +S+S I+ +PT LMHSMQGRVLPRYRVLQVVKSKRL +K P+ +  LG+S++DF DKFV 
Sbjct: 301 VSRSLIVRRPTSLMHSMQGRVLPRYRVLQVVKSKRLTRKNPELVDTLGISEEDFFDKFVY 360

Query: 301 RFPDNMKDLLVAFRGNSVGAFQPKEL 327
           RFPDN++DLLVA+ G  V A + KEL
Sbjct: 361 RFPDNVEDLLVAYSGQCVDALRRKEL 386

BLAST of Lsi07G001870 vs. NCBI nr
Match: XP_038888519.1 (transcription termination factor MTERF5, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 526.6 bits (1355), Expect = 1.6e-145
Identity = 264/320 (82.50%), Postives = 290/320 (90.62%), Query Frame = 0

Query: 1   MSCRKGVVSTEKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLG 60
           MSCR+G+ STEKPQSVYKYLS+LGFSDAHI+SAIRL PQIAFS+IEKTLKPKIEFFQNLG
Sbjct: 67  MSCRRGLQSTEKPQSVYKYLSDLGFSDAHIRSAIRLAPQIAFSNIEKTLKPKIEFFQNLG 126

Query: 61  LVGSDLGKFISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSP 120
           LVGSDL KFISRHS+LLTVSLEKKLMPSVEILKN+ PKDNCN DLL V+RR SD +MR+P
Sbjct: 127 LVGSDLSKFISRHSTLLTVSLEKKLMPSVEILKNILPKDNCNDDLLLVIRRWSDRLMRTP 186

Query: 121 YKRLSVNINYLQSCGIVDSQLSMLLKMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVH 180
            KRLS+NINYLQSCGIVDSQL MLLK Q + FG+ ESR+KDIVS+VVE GFST+T+MFVH
Sbjct: 187 CKRLSININYLQSCGIVDSQLLMLLKRQSSLFGRSESRLKDIVSVVVEIGFSTNTRMFVH 246

Query: 181 GLQAISCVSNATFNKKVDLICSFGITEKECMRMFTSAPFLIRTSIDKLKLGLEFFMNEAK 240
           GL AIS VS  T  KKV+LICSFGITEKECMRMFTSAP LIRTSI KLKLGLEFFMNEAK
Sbjct: 247 GLHAISSVSKVTLKKKVELICSFGITEKECMRMFTSAPVLIRTSIGKLKLGLEFFMNEAK 306

Query: 241 LSKSHILGKPTCLMHSMQGRVLPRYRVLQVVKSKRLLKKQPKFIHILGMSDQDFLDKFVC 300
           +SKS I+G P CLMH+MQGRVLPRYRVLQ+VKSKRL+KK PKFI  LGMSD+DFLD+FVC
Sbjct: 307 VSKSDIVGSPACLMHAMQGRVLPRYRVLQIVKSKRLMKKHPKFILTLGMSDEDFLDRFVC 366

Query: 301 RFPDNMKDLLVAFRGNSVGA 321
           RFPDNMKDLLVA+RGNSV A
Sbjct: 367 RFPDNMKDLLVAYRGNSVSA 386

BLAST of Lsi07G001870 vs. NCBI nr
Match: KAG7015351.1 (Transcription termination factor MTERF8, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 522.7 bits (1345), Expect = 2.3e-144
Identity = 264/328 (80.49%), Postives = 292/328 (89.02%), Query Frame = 0

Query: 1   MSCRKGVVSTEKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLG 60
           M  R+G+ STEKPQSVYKYLSELGFS AHIQS IRL PQIAFS+IE+TLKPKIEFFQNLG
Sbjct: 64  MRSRRGIKSTEKPQSVYKYLSELGFSKAHIQSTIRLAPQIAFSNIERTLKPKIEFFQNLG 123

Query: 61  LVGSDLGKFISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSP 120
           LVGSDLG+FIS+HS+LLTVSLEKKLMPSVEILK+VFPKD CN DLL VM+RCSD +MRSP
Sbjct: 124 LVGSDLGRFISKHSTLLTVSLEKKLMPSVEILKSVFPKDECNSDLLQVMQRCSDKLMRSP 183

Query: 121 YKRLSVNINYLQSCGIVDSQLSMLLKMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVH 180
           Y RL VNINYLQSCGIV SQLSMLLK QPA FGKRES+V+DIVSMVVETGFST+TKMFVH
Sbjct: 184 YTRLLVNINYLQSCGIVGSQLSMLLKRQPALFGKRESQVRDIVSMVVETGFSTNTKMFVH 243

Query: 181 GLQAISCVSNATFNKKVDLICSFGITEKECMRMFTSAPFLIRTSIDKLKLGLEFFMNEAK 240
           GL AIS V+NATF KKV+LICSFGITEKECMRMFTSAP LIRTS+ KLK GLEFFMNEA+
Sbjct: 244 GLHAISSVTNATFQKKVELICSFGITEKECMRMFTSAPVLIRTSVGKLKRGLEFFMNEAE 303

Query: 241 LSKSHILGKPTCLMHSMQGRVLPRYRVLQVVKSKRLLKKQPKFIHILGMSDQDFLDKFVC 300
           +S+S I+ KPTCLMH+MQGRVLPRYRVLQVVKSK LLKK P+ + ILGMSD+DFLDKFV 
Sbjct: 304 VSRSDIVRKPTCLMHAMQGRVLPRYRVLQVVKSKSLLKKHPQLLDILGMSDEDFLDKFVH 363

Query: 301 RFPDNMKDLLVAFRGNSVGAFQPKELTT 329
           RFPD++ +LL AFRG  +   QPKEL T
Sbjct: 364 RFPDHVTELLAAFRGQCMDELQPKELET 391

BLAST of Lsi07G001870 vs. NCBI nr
Match: XP_022984673.1 (uncharacterized protein LOC111482884 isoform X1 [Cucurbita maxima] >XP_022984674.1 uncharacterized protein LOC111482884 isoform X2 [Cucurbita maxima] >XP_022984675.1 uncharacterized protein LOC111482884 isoform X3 [Cucurbita maxima])

HSP 1 Score: 519.2 bits (1336), Expect = 2.5e-143
Identity = 261/328 (79.57%), Postives = 292/328 (89.02%), Query Frame = 0

Query: 1   MSCRKGVVSTEKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLG 60
           MS R+G+ STEKPQSVYKYLSELGFS+AHIQS IRL PQIAFS+IEKTLKPKIEFFQNLG
Sbjct: 64  MSSRRGIASTEKPQSVYKYLSELGFSEAHIQSTIRLAPQIAFSNIEKTLKPKIEFFQNLG 123

Query: 61  LVGSDLGKFISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSP 120
           LVGSDLG+FIS+HS+LLT+SLEK+LMPSVEILK+VFPKD CN DLL V+RRCSDM+MRSP
Sbjct: 124 LVGSDLGRFISKHSTLLTISLEKRLMPSVEILKSVFPKDECNSDLLQVLRRCSDMLMRSP 183

Query: 121 YKRLSVNINYLQSCGIVDSQLSMLLKMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVH 180
           Y RL VNINYLQSCGIV SQLSMLLK QPA FG RES+V+D+VSMVVETGFST+T+MFVH
Sbjct: 184 YTRLPVNINYLQSCGIVGSQLSMLLKRQPALFGMRESQVRDLVSMVVETGFSTNTRMFVH 243

Query: 181 GLQAISCVSNATFNKKVDLICSFGITEKECMRMFTSAPFLIRTSIDKLKLGLEFFMNEAK 240
           GL AIS VSNATF KKV+LICSFGITEKECMRMFTSAP LIRTS+ KLK GLEFFMNEA+
Sbjct: 244 GLHAISSVSNATFKKKVELICSFGITEKECMRMFTSAPVLIRTSVGKLKRGLEFFMNEAE 303

Query: 241 LSKSHILGKPTCLMHSMQGRVLPRYRVLQVVKSKRLLKKQPKFIHILGMSDQDFLDKFVC 300
           +S+S I+ KPTCLMH+MQGRVLPRYRVLQVV SKRL KK  + I ILGMSD++FLDKFV 
Sbjct: 304 VSRSDIVRKPTCLMHAMQGRVLPRYRVLQVVNSKRLSKKHLQLIDILGMSDENFLDKFVH 363

Query: 301 RFPDNMKDLLVAFRGNSVGAFQPKELTT 329
           RFPD++ +LL AFRG  +   QPKEL T
Sbjct: 364 RFPDHVTELLAAFRGQCMDELQPKELET 391

BLAST of Lsi07G001870 vs. NCBI nr
Match: XP_022929189.1 (uncharacterized protein LOC111435860 [Cucurbita moschata])

HSP 1 Score: 518.5 bits (1334), Expect = 4.3e-143
Identity = 262/328 (79.88%), Postives = 293/328 (89.33%), Query Frame = 0

Query: 1   MSCRKGVVSTEKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLG 60
           M  R+G+ STEKPQSVYKYLSELGFS AHIQS IRL PQIAFS+IE+TLKPKIEFFQNLG
Sbjct: 64  MRSRRGLESTEKPQSVYKYLSELGFSKAHIQSTIRLAPQIAFSNIERTLKPKIEFFQNLG 123

Query: 61  LVGSDLGKFISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSP 120
           LVGSDLG+FIS+HS+LLTVSLEK+LMPSVEILK+VFPKD CN DLL VM+RCSDM+MRSP
Sbjct: 124 LVGSDLGRFISKHSTLLTVSLEKRLMPSVEILKSVFPKDECNSDLLQVMQRCSDMLMRSP 183

Query: 121 YKRLSVNINYLQSCGIVDSQLSMLLKMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVH 180
           Y RL VNI+YLQSCGIV SQLSMLLK QPA FG RES+V+DIVSMVVETGFST+TKMFVH
Sbjct: 184 YTRLLVNISYLQSCGIVGSQLSMLLKRQPALFGMRESQVRDIVSMVVETGFSTNTKMFVH 243

Query: 181 GLQAISCVSNATFNKKVDLICSFGITEKECMRMFTSAPFLIRTSIDKLKLGLEFFMNEAK 240
           GL +IS VSNATF KKV+LICSFGITEKECMRMFTSAP LIRTS+ KLK GLEFFMNEA+
Sbjct: 244 GLHSISSVSNATFKKKVELICSFGITEKECMRMFTSAPGLIRTSVGKLKRGLEFFMNEAE 303

Query: 241 LSKSHILGKPTCLMHSMQGRVLPRYRVLQVVKSKRLLKKQPKFIHILGMSDQDFLDKFVC 300
           +S+S I+ KPTCLMH+MQGRVLPRYRVLQVVKSKRLL+K P+ + ILGMSD+DFLDKFV 
Sbjct: 304 VSRSDIVCKPTCLMHAMQGRVLPRYRVLQVVKSKRLLEKHPQLLDILGMSDEDFLDKFVH 363

Query: 301 RFPDNMKDLLVAFRGNSVGAFQPKELTT 329
           RFPD++ +LL AFRG  +   QPKEL T
Sbjct: 364 RFPDHVTELLAAFRGQCMDELQPKELET 391

BLAST of Lsi07G001870 vs. NCBI nr
Match: XP_023552363.1 (transcription termination factor MTEF1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 517.7 bits (1332), Expect = 7.3e-143
Identity = 261/328 (79.57%), Postives = 291/328 (88.72%), Query Frame = 0

Query: 1   MSCRKGVVSTEKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLG 60
           MS R+G+ STEKPQSVYKYLSELGFS AHIQS IRL PQIAFS+IE+TLKPKIEFFQNLG
Sbjct: 64  MSSRRGLESTEKPQSVYKYLSELGFSKAHIQSTIRLAPQIAFSNIERTLKPKIEFFQNLG 123

Query: 61  LVGSDLGKFISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSP 120
           LVGSDLG+F+S+HS+LLT+SLEK+LMPSVEILK+VFPKD CN DLL VM+RCSDM+MRSP
Sbjct: 124 LVGSDLGRFVSKHSTLLTISLEKRLMPSVEILKSVFPKDECNSDLLQVMQRCSDMLMRSP 183

Query: 121 YKRLSVNINYLQSCGIVDSQLSMLLKMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVH 180
           Y RL VNINYLQSCGIV SQLSMLLK QPA FG RES+V+DIVSMVVETGFST+TKMFVH
Sbjct: 184 YTRLLVNINYLQSCGIVGSQLSMLLKRQPALFGMRESQVRDIVSMVVETGFSTNTKMFVH 243

Query: 181 GLQAISCVSNATFNKKVDLICSFGITEKECMRMFTSAPFLIRTSIDKLKLGLEFFMNEAK 240
           GL +IS VSNATF KKV LICSFGITEKECMRMFTSAP LIRTS+ KLK GLEFFMNEA+
Sbjct: 244 GLHSISSVSNATFKKKVGLICSFGITEKECMRMFTSAPGLIRTSVGKLKRGLEFFMNEAE 303

Query: 241 LSKSHILGKPTCLMHSMQGRVLPRYRVLQVVKSKRLLKKQPKFIHILGMSDQDFLDKFVC 300
           +S+S I+ KPTCLMH+MQGRVLPRYRVLQVVKS RLLKK P+ + ILGMSD+DFLDKFV 
Sbjct: 304 VSRSDIVRKPTCLMHAMQGRVLPRYRVLQVVKSTRLLKKHPQLLDILGMSDEDFLDKFVH 363

Query: 301 RFPDNMKDLLVAFRGNSVGAFQPKELTT 329
           RFPD++ +LL AFRG  +   QP EL T
Sbjct: 364 RFPDHVTELLAAFRGQCMDELQPMELET 391

BLAST of Lsi07G001870 vs. TAIR 10
Match: AT5G64950.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.5e-61
Identity = 128/313 (40.89%), Postives = 207/313 (66.13%), Query Frame = 0

Query: 9   STEKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLGLVGSDLGK 68
           S E+P+SV + L    FSD  IQ +IR+ P++ F ++EK L+PK+ FF+++G  GS LGK
Sbjct: 67  SLEQPRSVIQMLKSYSFSDTQIQKSIRVHPRMMFYNVEKILEPKLRFFKDIGFTGSGLGK 126

Query: 69  FISRHSSLLTVSLEKKLMPSVEILKN-VFPKDNCNRDLLCVMRRCSDMVM-RSPYKRLSV 128
           F+S++SS++ VSL KKL+P+VEILK+ V PK   + DL  ++ RC  +++ R P   L  
Sbjct: 127 FVSQNSSVVGVSLVKKLIPTVEILKSIVAPK---HEDLPVILSRCGWLLLSRDPNLFLLP 186

Query: 129 NINYLQSCGIVDSQLSMLLKMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVHGLQAIS 188
           NI+YL++CGIV SQL+ LL+ QP  F   E +++  VS  ++ GF+ +++M VH + ++S
Sbjct: 187 NISYLETCGIVGSQLASLLRRQPRIFNLSEEKLRGYVSRALDLGFTLNSRMLVHAVISLS 246

Query: 189 CVSNATFNKKVDLICSFGITEKECMRMFTSAPFLIRTSIDKLKLGLEFFMNEAKLSKSHI 248
            +S  TF++KV L  + G +E E   +   +P LIR S DKL LG EF++    L +  +
Sbjct: 247 SLSEKTFDRKVKLFMANGFSEDEITDIIRRSPGLIRCSEDKLTLGFEFYLKRMGLEREAL 306

Query: 249 LGKPTCLMHSMQGRVLPRYRVLQVVKSKRLL----KKQPKFIHILGMSDQDFLDKFVCRF 308
             +P  L ++++ RV+PR +VLQ+++ K LL    KK+   + I+ M+++ FL+K+V RF
Sbjct: 307 AKRPCVLSYNLEKRVIPRLKVLQILREKGLLLKEEKKKKNMVGIVEMTEEAFLEKYVVRF 366

Query: 309 PDNM-KDLLVAFR 315
            D + ++LLVA++
Sbjct: 367 GDEIAEELLVAYK 376

BLAST of Lsi07G001870 vs. TAIR 10
Match: AT1G21150.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 154.1 bits (388), Expect = 2.0e-37
Identity = 90/311 (28.94%), Postives = 157/311 (50.48%), Query Frame = 0

Query: 7   VVSTEKPQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLGLVGSDL 66
           +VS++KP SV     + GF++  I S I+  P++   S E  + PK+ FF ++G   SD 
Sbjct: 82  LVSSKKPDSVLALFKDHGFTNDQITSVIKSFPRVLSLSPEDVISPKLMFFSSIGFSTSDT 141

Query: 67  GKFISRHSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSPYKRLSV 126
            K IS    +L+ SL K+L+P  + LK++  ++      L    RC  + +      +S+
Sbjct: 142 AKMISSSPKMLSYSLHKRLIPCYDSLKSILVEEESVVKCLKRGIRCFSLKIT---HCVSL 201

Query: 127 NINYLQSCGIVDSQLSMLLKMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVHGLQAIS 186
            ++  +  G+ D  +  L++  P  F  RE R  ++++ V   GF      FVH + A  
Sbjct: 202 RVSICRELGVPDKSIKWLVQASPFTFFSRERRFNEVLNRVCSYGFDPKKAGFVHAMVAFD 261

Query: 187 CVSNATFNKKVDLICSFGITEKECMRMFTSAPFLIRTSIDKLKLGLEFFMNEAKLSKSHI 246
           C S +   +K  L   FG ++++ +      P  +  S +K+   LE+ +N   L    I
Sbjct: 262 CTSESAMERKFKLFQCFGWSKEDFVAAIMRFPNCVTVSDEKIMYTLEYLVNNIGLQARDI 321

Query: 247 LGKPTCLMHSMQGRVLPRYRVLQVVKSKRLLKKQP-KFIHILGMSDQDFLDKFVCRFPDN 306
           + +P  L  SM+ R+ PR +V+ ++ SK L+KK+   +  IL +   +F+DKFV ++ D 
Sbjct: 322 VARPVVLSLSMEKRIKPRNQVISLLLSKGLVKKEDINYFTILKLKSSEFMDKFVLKYQDE 381

Query: 307 MKDLLVAFRGN 317
           M  L+  F  N
Sbjct: 382 MPQLVQPFTSN 389

BLAST of Lsi07G001870 vs. TAIR 10
Match: AT1G62120.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 88.2 bits (217), Expect = 1.3e-17
Identity = 84/351 (23.93%), Postives = 143/351 (40.74%), Query Frame = 0

Query: 13  PQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLGLVGSDLGKFISR 72
           P SV   L   GF+D+ I + IR  P++     EK+L PK++F Q++G   S+L + +S 
Sbjct: 88  PDSVLSLLRSHGFTDSQISNIIRTFPRLLILDAEKSLAPKLQFLQSIGASSSELTETVSA 147

Query: 73  HSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSPYKRLSVNINYLQ 132
              +L     K L    + +K +   D  ++    + + C  +   S  +    N+  L+
Sbjct: 148 VPKILGKRKGKSLSRYYDFVKVIIEADKSSK----LEKLCHSLPEGSKQENKIRNLLVLR 207

Query: 133 SCGIVDSQL-SMLLKMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVHGLQAISCVSN- 192
             G+    L S+L+       GK   + K+ +   VE GF   T  FV  L  +  +S+ 
Sbjct: 208 EMGVPQRLLFSLLISDAGDVCGK--EKFKESLKKAVEIGFDPTTATFVKALNVLYGLSDK 267

Query: 193 --------------------ATFNK--------------KVDLICSFGITEKECMRMFTS 252
                               A F K               V+     G +  E + M   
Sbjct: 268 GIENKFNACKRLGLAVDDVWAMFKKWPNILTKSEKKIENSVETFLGLGFSRDEFLMMVKR 327

Query: 253 APFLIRTSIDKLKLGLEFFMNEAKLSKSHILGKPTCLMHSMQGRVLPRYRVLQVVKSKRL 312
            P  I  S + +K   EF + E       +   P  L +S++ R +PR  V++V+ SK L
Sbjct: 328 FPQCIGYSTELMKTKTEFLVTEMNWPLKAVASIPQVLGYSLEKRTVPRCNVIKVLISKGL 387

Query: 313 LKKQ-PKFIHILGMSDQDFLDKFVCRFPDN--MKDLLVAFRGNSVGAFQPK 325
           L+ + P    +L  + + FL  +V +  D   + +L+  F G+ V     K
Sbjct: 388 LESELPPISSVLTSTSEVFLYMYVRKHDDKQLVAELMAIFTGDRVSLTDQK 432

BLAST of Lsi07G001870 vs. TAIR 10
Match: AT1G62010.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 85.9 bits (211), Expect = 6.6e-17
Identity = 88/350 (25.14%), Postives = 145/350 (41.43%), Query Frame = 0

Query: 15  SVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLGLVGSDLGKFISRHS 74
           SV   L   GF+D+ I S IR   ++   +   +L  K++F Q+ G   S+L + +S   
Sbjct: 72  SVLDLLRSYGFTDSQISSIIRSDSRVLIDNDATSLGSKLQFLQSRGASSSELTEVVSTVP 131

Query: 75  SLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSPYKRLSVNINYLQSC 134
            +L     K L    + +K +   D  ++       + S  + +    R   NI  L+  
Sbjct: 132 KILGKREGKSLSRYYDFIKVIIEADKSSK-----YEKISHSLAQGNKIR---NILVLREL 191

Query: 135 GIVDSQLSMLL--KMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVHGLQAISCVSNAT 194
           G+   +L +LL  K QP   GK +      +  VVE GF   T  FVH L  +  +S+ T
Sbjct: 192 GVPQKRLLLLLISKSQPV-CGKEKFDAS--LKKVVEMGFDPTTSTFVHALHMLYQMSDKT 251

Query: 195 FNKKVDLICSFGI------------------TEKEC-----------------MRMFTSA 254
             +K+ +  S G                   +EK+                  M MF   
Sbjct: 252 IEEKIRVYRSVGFSVDDVWAMFKKWPRSLTHSEKKVANSIETFLGLGFSRDVFMMMFKRF 311

Query: 255 PFLIRTSIDKLKLGLEFFMNEAKLSKSHILGKPTCLMHSMQGRVLPRYRVLQVVKSKRLL 314
           P  I  S + +K   EF + E       +   P  L +S++ R +PR  V++V+ SK LL
Sbjct: 312 PPCIGYSTEAVKKKTEFLVKEMNWPVKAVASIPQVLGYSLEKRTVPRCNVIKVLMSKGLL 371

Query: 315 KKQ-PKFIHILGMSDQDFLDKFVCRFPDN--MKDLLVAFRGNSVGAFQPK 325
           + + P    +L  + + FL+ +V +  D   + +L+  F GN V     K
Sbjct: 372 ESELPPMSSVLTSTSESFLNLYVSKHDDKQLVAELMAIFTGNRVSLTDQK 410

BLAST of Lsi07G001870 vs. TAIR 10
Match: AT1G61990.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 81.3 bits (199), Expect = 1.6e-15
Identity = 78/346 (22.54%), Postives = 144/346 (41.62%), Query Frame = 0

Query: 13  PQSVYKYLSELGFSDAHIQSAIRLVPQIAFSSIEKTLKPKIEFFQNLGLVGSDLGKFISR 72
           P SV       GF+D+ I + I   P +  +  +K L  K++  Q+ G   S++ + +S 
Sbjct: 84  PDSVLSLFRSYGFTDSQISTIITDYPLLLVADAKKALGRKLQILQSRGASSSEITEIVST 143

Query: 73  HSSLLTVSLEKKLMPSVEILKNVFPKDNCNRDLLCVMRRCSDMVMRSPYKRLSVNINYLQ 132
              +L    +K +    + +K++   D  +           ++   S   ++  N++ L+
Sbjct: 144 VPRILG---KKSITVYYDAVKDIIVADTSS---------SYELPQGSQGNKIR-NVSALR 203

Query: 133 SCGIVDSQLSMLL--KMQPAFFGKRESRVKDIVSMVVETGFSTDTKMFVHGLQAISCVSN 192
             G+    L  LL  K QP   GK        +  VVE GF   T  FV  L+ +  +S 
Sbjct: 204 ELGMPSRLLLPLLVSKSQPV-CGKENFDAS--LKKVVEMGFDPTTTKFVLALRMLYQMSE 263

Query: 193 ATFNKKVDLICSFGITEKECMRMFTSAPFLIRT--------------------------- 252
            T  +KV +  S G T  +   +F   P +++                            
Sbjct: 264 KTIEEKVVVFRSLGFTVDDVWEIFKKTPSVLKVSKKKILKSAETFLDLGYSRAEFLMMVK 323

Query: 253 --------SIDKLKLGLEFFMNEAKLSKSHILGKPTCLMHSMQGRVLPRYRVLQVVKSKR 312
                   S++ +K   EF + + K  ++ ++  P    +SM+ R++PR  +L+ + SK 
Sbjct: 324 RYPPCIEYSVESVKKKNEFLVKKMKWPRNALVLHPQVFGYSMEKRIIPRCNILEALLSKG 383

Query: 313 LLKK---QPKFIHILGMSDQDFLDKFVCRFPDNMKDLLVAFRGNSV 319
           LL+K    P    +L  +D+ FLD++V +  + +  L+  F   SV
Sbjct: 384 LLRKGSELPAVSSVLSCTDEGFLDRYVMKHNELVPTLMAIFTKGSV 413

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JVI32.5e-0828.48Transcription termination factor MTERF5, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9FM801.2e-0722.19Transcription termination factor MTERF9, chloroplastic OS=Arabidopsis thaliana O... [more]
F4IHL32.6e-0523.49Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9SZL62.6e-0524.00Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidop... [more]
Q84X535.7e-0531.03Transcription termination factor MTEF1, chloroplastic OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A6J1JB801.2e-14379.57uncharacterized protein LOC111482884 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1EN212.1e-14379.88uncharacterized protein LOC111435860 OS=Cucurbita moschata OX=3662 GN=LOC1114358... [more]
A0A6J1END23.9e-14279.27uncharacterized protein LOC111436067 OS=Cucurbita moschata OX=3662 GN=LOC1114360... [more]
A0A6J1J5Y44.3e-14179.94uncharacterized protein LOC111482884 isoform X4 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1C8W12.6e-13878.22uncharacterized protein LOC111008506 isoform X1 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
XP_038888519.11.6e-14582.50transcription termination factor MTERF5, chloroplastic-like [Benincasa hispida][more]
KAG7015351.12.3e-14480.49Transcription termination factor MTERF8, chloroplastic, partial [Cucurbita argyr... [more]
XP_022984673.12.5e-14379.57uncharacterized protein LOC111482884 isoform X1 [Cucurbita maxima] >XP_022984674... [more]
XP_022929189.14.3e-14379.88uncharacterized protein LOC111435860 [Cucurbita moschata][more]
XP_023552363.17.3e-14379.57transcription termination factor MTEF1, chloroplastic-like [Cucurbita pepo subsp... [more]
Match NameE-valueIdentityDescription
AT5G64950.11.5e-6140.89Mitochondrial transcription termination factor family protein [more]
AT1G21150.12.0e-3728.94Mitochondrial transcription termination factor family protein [more]
AT1G62120.11.3e-1723.93Mitochondrial transcription termination factor family protein [more]
AT1G62010.16.6e-1725.14Mitochondrial transcription termination factor family protein [more]
AT1G61990.11.6e-1522.54Mitochondrial transcription termination factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 142..172
e-value: 19.0
score: 14.2
coord: 31..62
e-value: 0.012
score: 24.8
coord: 244..274
e-value: 2100.0
score: 1.4
coord: 67..99
e-value: 2.5
score: 17.1
coord: 106..137
e-value: 140.0
score: 10.6
coord: 211..242
e-value: 29.0
score: 13.6
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 106..199
e-value: 1.7E-6
score: 27.2
coord: 18..98
e-value: 9.3E-12
score: 44.5
coord: 124..295
e-value: 4.8E-20
score: 71.7
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 8..315
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 101..307
e-value: 6.0E-36
score: 125.9
NoneNo IPR availablePANTHERPTHR13068:SF173EMB|CAB62602.1coord: 8..315

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G001870.1Lsi07G001870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006353 DNA-templated transcription, termination
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003690 double-stranded DNA binding