Lsi07G000600 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi07G000600 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 435.3 bits (1118), Expect = 1.8e-120
Identity = 326/905 (36.02%), Postives = 480/905 (53.04%), Query Frame = 0

Query: 16  QIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLEDIVFKADVLLDEL 75
           ++GL +GF+K+  K+     M+QA+L D    +  Y  +K W++KL    ++ D +LD+ 
Sbjct: 20  ELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDC 79

Query: 76  AYEDLRRKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQEHYAAASVAGLV 135
             E  R K  +   +G+            + F  K+  R+K    M+++  A A      
Sbjct: 80  KTEAARFKQAV---LGR-------YHPRTITFCYKVGKRMK---EMMEKLDAIAEERRNF 139

Query: 136 AITSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEMALSVLPIVGMGG 195
            +  +  E   ++  +T   L E  V GR++E  EIV  LI   +    + VLPI+GMGG
Sbjct: 140 HLDERIIERQAAR-RQTGFVLTEPKVYGREKEEDEIVKILINNVSYSEEVPVLPILGMGG 199

Query: 196 LGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPNSGGLESKEALLQ 255
           LGKT LA+++FN + I E+F+  IWVCVSD F   ++++AI+E++   S G      L +
Sbjct: 200 LGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSLGDMDLAPLQK 259

Query: 256 EIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTSRSDEVAKIMETH 315
           ++Q+LL+ K YFLVLDDVWNE+   W+ L+A +LKI    G  +L+T+R +++  IM T 
Sbjct: 260 KLQELLNGKRYFLVLDDVWNEDQEKWDNLRA-VLKIGAS-GASILITTRLEKIGSIMGTL 319

Query: 316 PRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPLVVKVLGGTVKLD 375
             + L  LS + CW LF++ AF     T PKL +I +E+V+  GGVPL  K LGG ++  
Sbjct: 320 QLYQLSNLSQEDCWLLFKQRAFCHQTETSPKLMEIGKEIVKKCGGVPLAAKTLGGLLRFK 379

Query: 376 KNYERLQS-RLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCSNFPRDFEFRKD 435
           +     +  R + + ++P QDEN VL  ++LS   LP   L+QCFAYC+ FP+D +  K+
Sbjct: 380 REESEWEHVRDSEIWNLP-QDENSVLPALRLSYHHLP-LDLRQCFAYCAVFPKDTKIEKE 439

Query: 436 ALIQMWIAQGFIQPPEERNVTMEDVGDKYFNML-SRSLFQDVIKDKRGRTIFCKMHDFIH 495
            LI +W+A  F+    + N+ +EDVG++ +N L  RS FQ+ I+ K G+T F KMHD IH
Sbjct: 440 YLIALWMAHSFLL--SKGNMELEDVGNEVWNELYLRSFFQE-IEVKSGKTYF-KMHDLIH 499

Query: 496 DVACAI---SSDGRRTFDYSDKDARKLHMFTLGRYVIHPEIA--------------NFVY 555
           D+A ++   S+  R     + KD   + MF +  Y     I                FV 
Sbjct: 500 DLATSMFSASASSRSIRQINVKDDEDM-MFIVTNYKDMMSIGFSEVVSSYSPSLFKRFVS 559

Query: 556 LRVLIVHSYFIYELPNSIAKLKYLRYIDISDSAIWKLPESLVLLYNLQTLRL--GRFIKY 615
           LRVL + +    +LP+S+  L +LRY+D+S + I  LP+ L  L NLQTL L   + +  
Sbjct: 560 LRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGNKICSLPKRLCKLQNLQTLDLYNCQSLSC 619

Query: 616 LPNDLRKLVSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSSFVVEFHDGYKIEELGFLK 675
           LP    KL SLR+L     P      MP  +G L  L+TL  FVV    GY++ EL  L 
Sbjct: 620 LPKQTSKLCSLRNLVLDHCP---LTSMPPRIGLLTCLKTLGYFVVGERKGYQLGELRNL- 679

Query: 676 NLKGKLSLLCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKHERESSSYNDLDVLEGLQP 735
           NL+G +S+  LERVKN  EA  A L  K ++  L   W   +  ES    ++ VLE L+P
Sbjct: 680 NLRGAISITHLERVKNDMEAKEANLSAKANLHSLSMSWDRPNRYES---EEVKVLEALKP 739

Query: 736 HKNLQALRIQNFAGQLLP---SRIFVENLVEIYLQDCERCETLPMLGQLSKLEVLEIRNL 795
           H NL+ L I +F G  LP   +   ++N+V I +  CE C  LP  G+L  LE LE+++ 
Sbjct: 740 HPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCENCSCLPPFGELPCLESLELQD- 799

Query: 796 PSVRRIGDEFYGNYCRERTLFPELKTFHIFEMKSLEHWEEIASISNGIMFPHLESLSIIC 855
            SV     E  G   R R  FP L+  HI    +L+  + +        FP LE + I  
Sbjct: 800 GSVEVEYVEDSGFLTRRR--FPSLRKLHIGGFCNLKGLQRMKGAE---QFPVLEEMKISD 859

Query: 856 CVKLTNIPNLFASCWKRLEV--DVDSASLSSSRGHGKLRSLKILGCEKLTKLWNGLEFCN 895
           C      P L  S  K+LE+  + D+  LSS      L SLKI     +T L    E   
Sbjct: 860 CPMFV-FPTL--SSVKKLEIWGEADAGGLSSISNLSTLTSLKIFSNHTVTSLLE--EMFK 883

BLAST of Lsi07G000600 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 2.3e-112
Identity = 321/931 (34.48%), Postives = 481/931 (51.66%), Query Frame = 0

Query: 16  QIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLEDIVFKADVLLDEL 75
           ++ L +GF+ +  ++      +QA+L D    + +   ++ W++KL    ++ D +LDE 
Sbjct: 20  ELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAATYEVDDILDEY 79

Query: 76  AYEDLR-RKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQEHYAAASVAGL 135
             +  R  + E G    K +            FR K+  R+  + + L+   A A     
Sbjct: 80  KTKATRFSQSEYGRYHPKVI-----------PFRHKVGKRMDQVMKKLK---AIAEERKN 139

Query: 136 VAITSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEMALSVLPIVGMG 195
             +  K  E    +  ET S L E  V GRD+E  EIV  LI   +    LSVLPI+GMG
Sbjct: 140 FHLHEKIVERQAVR-RETGSVLTEPQVYGRDKEKDEIVKILINNVSDAQHLSVLPILGMG 199

Query: 196 GLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETL--NPNSGGLESKEA 255
           GLGKT LA+++FN + + E+F   IW+CVS+ F   ++++AI+E++   P  G ++    
Sbjct: 200 GLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESIEGRPLLGEMD-LAP 259

Query: 256 LLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTSRSDEVAKIM 315
           L +++Q+LL+ K Y LVLDDVWNE+   W  L+A +LK+    G  VL T+R ++V  IM
Sbjct: 260 LQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRA-VLKVGAS-GASVLTTTRLEKVGSIM 319

Query: 316 ETHPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPLVVKVLGGTV 375
            T   + L  LS + CW LF + AFG      P L  I +E+V+  GGVPL  K LGG +
Sbjct: 320 GTLQPYELSNLSQEDCWLLFMQRAFGHQEEINPNLVAIGKEIVKKSGGVPLAAKTLGG-I 379

Query: 376 KLDKNYERLQSRLTN--LMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCSNFPRDFE 435
              K  ER    + +  + ++P QDE+ +L  ++LS  +LP   LKQCFAYC+ FP+D +
Sbjct: 380 LCFKREERAWEHVRDSPIWNLP-QDESSILPALRLSYHQLP-LDLKQCFAYCAVFPKDAK 439

Query: 436 FRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFNML-SRSLFQDVIKDKRGRTIFCKMH 495
             K+ LI +W+A GF+    + N+ +EDVGD+ +  L  RS FQ+ I+ K G+T F KMH
Sbjct: 440 MEKEKLISLWMAHGFLL--SKGNMELEDVGDEVWKELYLRSFFQE-IEVKDGKTYF-KMH 499

Query: 496 DFIHDVACAISSDGRRTFDYSDKDARK-LHMFTLG-----RYVIHPEIANFVYLRVLIVH 555
           D IHD+A ++ S    + +  + +     HM ++G      +   P +  F+ LRVL + 
Sbjct: 500 DLIHDLATSLFSANTSSSNIREINKHSYTHMMSIGFAEVVFFYTLPPLEKFISLRVLNLG 559

Query: 556 SYFIYELPNSIAKLKYLRYIDISDSAIWKLPESLVLLYNLQTLRLGRFIKY--LPNDLRK 615
                +LP+SI  L +LRY+++  S +  LP+ L  L NLQTL L    K   LP +  K
Sbjct: 560 DSTFNKLPSSIGDLVHLRYLNLYGSGMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSK 619

Query: 616 LVSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSSFVVEFHDGYKIEELGFLKNLKGKLS 675
           L SLR+L    D S     MP  +G L  L+TL  FVV    GY++ ELG L NL G + 
Sbjct: 620 LGSLRNL--LLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRKKGYQLGELGNL-NLYGSIK 679

Query: 676 LLCLERVKNKKEAMAAKLVEKEHIFHLDFQWG--WKHERESSSYNDLDVLEGLQPHKNLQ 735
           +  LERVKN K+A  A L  K ++  L   W     H  ES    ++ VLE L+PH NL 
Sbjct: 680 ISHLERVKNDKDAKEANLSAKGNLHSLSMSWNNFGPHIYES---EEVKVLEALKPHSNLT 739

Query: 736 ALRIQNFAGQLLP---SRIFVENLVEIYLQDCERCETLPMLGQLSKLEVLEIR----NLP 795
           +L+I  F G  LP   +   ++N+V I + +   C  LP  G L  LE LE+     ++ 
Sbjct: 740 SLKIYGFRGIHLPEWMNHSVLKNIVSILISNFRNCSCLPPFGDLPCLESLELHWGSADVE 799

Query: 796 SVRRIGDEFYGNYCRERTLFPELKTFHIFEMKSLEHWEEIASISNGIMFPHLESLSIICC 855
            V  +  + +  +   R  FP L+   I++  SL   + +        FP LE + I  C
Sbjct: 800 YVEEVDIDVHSGF-PTRIRFPSLRKLDIWDFGSL---KGLLKKEGEEQFPVLEEMIIHEC 859

Query: 856 VKLTNIPNLFASCWKRLEVDVDSASLSSS--RGHGKLRSLKILGCEKLTKLWNGLEFCNS 915
             LT   NL A    R+  +  + S      +    L+ L I  C  L +L   L   N+
Sbjct: 860 PFLTLSSNLRALTSLRICYNKVATSFPEEMFKNLANLKYLTISRCNNLKELPTSLASLNA 915

BLAST of Lsi07G000600 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 5.3e-109
Identity = 324/960 (33.75%), Postives = 481/960 (50.10%), Query Frame = 0

Query: 5   VLKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLEDI 64
           +L+       +++ L +GF+K+  K+      +QA+L+D    +     ++ W++KL   
Sbjct: 9   LLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKLNSA 68

Query: 65  VFKADVLLDELAYEDLRRKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQE 124
            ++ D +L E   E +R +      +G      I+       FR K+  R+K I   L  
Sbjct: 69  AYEVDDILGECKNEAIRFE---QSRLGFYHPGIIN-------FRHKIGRRMKEIMEKLD- 128

Query: 125 HYAAASVAGLVAITSKETEPD-LSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEM 184
             A +          K TE    +   ET   L E  V GRD+E  EIV  LI   N   
Sbjct: 129 --AISEERRKFHFLEKITERQAAAATRETGFVLTEPKVYGRDKEEDEIVKILINNVNVAE 188

Query: 185 ALSVLPIVGMGGLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPN 244
            L V PI+GMGGLGKT LA++IFN E + ++F+  IWVCVSD F   ++++ I+  +  +
Sbjct: 189 ELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIERS 248

Query: 245 SGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTS 304
           S  +E   +  +++Q+LL+ K Y LVLDDVWN++L  W +L+A L    R  G  +L T+
Sbjct: 249 SPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGAR--GASILATT 308

Query: 305 RSDEVAKIMETHPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPL 364
           R ++V  IM T   +HL  LS      LF + AFG      P L  I +E+V+  GGVPL
Sbjct: 309 RLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVKKCGGVPL 368

Query: 365 VVKVLGGTVKLDKNYERLQS-RLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYC 424
             K LGG ++  +     +  R   + S+P QDE+ +L  ++LS   LP   L+QCFAYC
Sbjct: 369 AAKTLGGLLRFKREESEWEHVRDNEIWSLP-QDESSILPALRLSYHHLP-LDLRQCFAYC 428

Query: 425 SNFPRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFNML-SRSLFQDVIKDKRG 484
           + FP+D +  K+ LI +W+A GF+    + N+ +EDVG++ +N L  RS FQ+ I+ K G
Sbjct: 429 AVFPKDTKMIKENLITLWMAHGFLL--SKGNLELEDVGNEVWNELYLRSFFQE-IEAKSG 488

Query: 485 RTIFCKMHDFIHDVACAISSDGRRTFDYSDKDARKL-HMFTLGRYVIHPE-----IANFV 544
            T F K+HD IHD+A ++ S      +  + + +   H  ++G   +        +  FV
Sbjct: 489 NTYF-KIHDLIHDLATSLFSASASCGNIREINVKDYKHTVSIGFAAVVSSYSPSLLKKFV 548

Query: 545 YLRVLIVHSYFIYELPNSIAKLKYLRYIDISDSAIWKLPESLVLLYNLQTLRLGR--FIK 604
            LRVL +    + +LP+SI  L +LRY+D+S +    LPE L  L NLQTL +     + 
Sbjct: 549 SLRVLNLSYSKLEQLPSSIGDLLHLRYLDLSCNNFRSLPERLCKLQNLQTLDVHNCYSLN 608

Query: 605 YLPNDLRKLVSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSSFVVEFHDGYKIEELGFL 664
            LP    KL SLRHL     P       P  +G L  L+TL  F+V    GY++ EL  L
Sbjct: 609 CLPKQTSKLSSLRHLVVDGCP---LTSTPPRIGLLTCLKTLGFFIVGSKKGYQLGELKNL 668

Query: 665 KNLKGKLSLLCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKHERESSSYNDLDVLEGLQ 724
            NL G +S+  LERVKN  +A  A L  K ++  L   W         S  ++ VLE L+
Sbjct: 669 -NLCGSISITHLERVKNDTDA-EANLSAKANLQSLSMSWDNDGPNRYES-KEVKVLEALK 728

Query: 725 PHKNLQALRIQNFAGQLLPSRI---FVENLVEIYLQDCERCETLPMLGQLSKLEVLEIRN 784
           PH NL+ L I  F G   PS I    +E ++ + ++ C+ C  LP  G+L  LE LE++N
Sbjct: 729 PHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSCKNCLCLPPFGELPCLENLELQN 788

Query: 785 -LPSVRRIGDEFYGNYCRERTLFPELKTFHIFEMKSLEHWEEIASISNGIMFPHLESLSI 844
               V  + ++   +    R  FP LK   I+  +SL   + +        FP LE ++I
Sbjct: 789 GSAEVEYVEEDDVHSRFSTRRSFPSLKKLRIWFFRSL---KGLMKEEGEEKFPMLEEMAI 848

Query: 845 ICCVKLTNIPNLFASCWKRLEV--DVDSASLSSSRGHGKLRSLKI--------LGCEKLT 904
           + C  L   P L  S  K+LEV  + ++  LSS      L SL+I        L  E  T
Sbjct: 849 LYC-PLFVFPTL--SSVKKLEVHGNTNTRGLSSISNLSTLTSLRIGANYRATSLPEEMFT 908

Query: 905 KLWNGLEF------------------CNSLENLWISNCSNL------NLQNMHSLTQEFV 916
            L N LEF                   N+L+ L I +C +L       L+ + SLTQ FV
Sbjct: 909 SLTN-LEFLSFFDFKNLKDLPTSLTSLNALKRLQIESCDSLESFPEQGLEGLTSLTQLFV 934

BLAST of Lsi07G000600 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 381.3 bits (978), Expect = 3.0e-104
Identity = 304/907 (33.52%), Postives = 462/907 (50.94%), Query Frame = 0

Query: 5   VLKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLEDI 64
           VL         ++ L +GF+ +  ++      +QA+L D    + +   ++ W++KL   
Sbjct: 9   VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAA 68

Query: 65  VFKADVLLDELAYEDLR-RKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQ 124
            ++ D +LDE   +  R  + E G    K +            FR K+  R+  + + L 
Sbjct: 69  TYEVDDILDEYKTKATRFLQSEYGRYHPKVI-----------PFRHKVGKRMDQVMKKLN 128

Query: 125 EHYAAASVAGLVAITSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEM 184
              A A       +  K  E   +   ET S L E  V GRD+E  EIV  LI  ++   
Sbjct: 129 ---AIAEERKKFHLQEKIIERQ-AATRETGSVLTEPQVYGRDKEKDEIVKILINTASDAQ 188

Query: 185 ALSVLPIVGMGGLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPN 244
            LSVLPI+GMGGLGKT L++++FN + + E F   IW+C+SD F   ++++AI+E++   
Sbjct: 189 KLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIEGK 248

Query: 245 SGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTS 304
           S        L +++Q+LL+ K YFLVLDDVWNE+   W  L+A +LK+    G  VL T+
Sbjct: 249 SLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRA-VLKVGAS-GAFVLTTT 308

Query: 305 RSDEVAKIMETHPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPL 364
           R ++V  IM T   + L  LS + CW LF + AFG      P L  I +E+V+  GGVPL
Sbjct: 309 RLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKEIVKKCGGVPL 368

Query: 365 VVKVLGGTVKLDKNYERLQS-RLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYC 424
             K LGG ++  +     +  R + + ++P QDE+ +L  ++LS   LP   L+QCF YC
Sbjct: 369 AAKTLGGILRFKREEREWEHVRDSPIWNLP-QDESSILPALRLSYHHLP-LDLRQCFVYC 428

Query: 425 SNFPRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFNML-SRSLFQDVIKDKRG 484
           + FP+D +  K+ LI  W+A GF+    + N+ +EDVG++ +N L  RS FQ+ I+ + G
Sbjct: 429 AVFPKDTKMAKENLIAFWMAHGFLL--SKGNLELEDVGNEVWNELYLRSFFQE-IEVESG 488

Query: 485 RTIFCKMHDFIHDVACAISSDGRRTFDYSDKDAR-KLHMFTLGRYVIHPE-----IANFV 544
           +T F KMHD IHD+A ++ S    + +  + +A    +M ++G   +        +  FV
Sbjct: 489 KTYF-KMHDLIHDLATSLFSANTSSSNIREINANYDGYMMSIGFAEVVSSYSPSLLQKFV 548

Query: 545 YLRVLIVHSYFIYELPNSIAKLKYLRYIDISDS-AIWKLPESLVLLYNLQTLRLG--RFI 604
            LRVL + +  + +LP+SI  L +LRY+D+S +  I  LP+ L  L NLQTL L     +
Sbjct: 549 SLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCDSL 608

Query: 605 KYLPNDLRKLVSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSSFVVEFHDGYKIEELGF 664
             LP    KL SLR+L             P  +G L  L++LS FV+    G+++ EL  
Sbjct: 609 SCLPKQTSKLGSLRNLLL---DGCSLTSTPPRIGLLTCLKSLSCFVIGKRKGHQLGELKN 668

Query: 665 LKNLKGKLSLLCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKHERESSSYNDLDVLEGL 724
           L NL G +S+  L+RVK   +A  A L  K ++  L   W    + +     D +VLE L
Sbjct: 669 L-NLYGSISITKLDRVKKDTDAKEANLSAKANLHSLCLSW----DLDGKHRYDSEVLEAL 728

Query: 725 QPHKNLQALRIQNFAGQLLP---SRIFVENLVEIYLQDCERCETLPMLGQLSKLEVLEIR 784
           +PH NL+ L I  F G  LP   ++  ++N+V I ++ CE C  LP  G+L  LE LE+ 
Sbjct: 729 KPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENCSCLPPFGELPCLESLELH 788

Query: 785 -NLPSVRRIGDEFYGNYCRERTLFPELKTFHIFEMKSLEHWEEIASISNGIMFPHLESLS 844
                V  + D  +         FP L+   I++  +L   + +  +     FP LE ++
Sbjct: 789 TGSADVEYVEDNVHPG------RFPSLRKLVIWDFSNL---KGLLKMEGEKQFPVLEEMT 848

Query: 845 IICCVKLTNIPNLFASCWKRLEVDV-DSASLSSSRGHGKLRSLKILGCEKLTKLWNGLEF 895
              C     IP L  S  K L+V V D+  L S      L SL I    + T L    E 
Sbjct: 849 FYWCPMFV-IPTL--SSVKTLKVIVTDATVLRSISNLRALTSLDISDNVEATSLPE--EM 870

BLAST of Lsi07G000600 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 7.5e-103
Identity = 284/962 (29.52%), Postives = 489/962 (50.83%), Query Frame = 0

Query: 27  LSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLEDIVFKADVLLDELAYEDLRRKVEI 86
           L ++  +LL + A+L D +  +   P V+ WV +L D+V+ A+  LD++A E LR  +  
Sbjct: 39  LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98

Query: 87  GEDIGKKVR---------NFISASENPLVFRLKMANRIKAISRMLQEHYAAASVAGLVAI 146
                 ++R         +F+  +   L        R++ ++  L+   +  ++ GL  +
Sbjct: 99  ESSSSNRLRQLRGRMSLGDFLDGNSEHL------ETRLEKVTIRLERLASQRNILGLKEL 158

Query: 147 TSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEMALSVLPIVGMGGLG 206
           T+   +  L     T S +DE  V GRD++  EI+  LI  +  +  ++V+ IVG+GG+G
Sbjct: 159 TAMIPKQRL----PTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVG 218

Query: 207 KTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPNSGGLESKEALLQEI 266
           KT L+++++N + +R  F   +W  VS+ F + KI + + E++          + L  ++
Sbjct: 219 KTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKL 278

Query: 267 QKLLHDKN--YFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTSRSDEVAKIMETH 326
           ++ L      + LVLDD+WNEN + W+ L+   +   +  G+ +LVT+RS  VA IM   
Sbjct: 279 KERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQ--GSQILVTTRSQRVASIMCAV 338

Query: 327 PRHHLKKLSNDHCWSLFEKCAFGSDLPTIPK-LDKIRRELVENFGGVPLVVKVLGGTVKL 386
             H+L+ LS+  CWSLF K  FG+  P + + +  +   +V    G+PL VK LGG ++ 
Sbjct: 339 HVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRF 398

Query: 387 DK---NYER-LQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCSNFPRDFE 446
           +     +ER L SR+ +L +    D++ +L  +++S   LP + LK+CFAYCS FP+   
Sbjct: 399 EGKVIEWERVLSSRIWDLPA----DKSNLLPVLRVSYYYLP-AHLKRCFAYCSIFPKGHA 458

Query: 447 FRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFNML-SRSLFQDVIKDKRGRTIFCKMH 506
           F KD ++ +W+A+GF+Q     +  +E++G++YF+ L SRSL Q      + +T +  MH
Sbjct: 459 FEKDKVVLLWMAEGFLQQTRS-SKNLEELGNEYFSELESRSLLQ------KTKTRYI-MH 518

Query: 507 DFIHDVA--------------CAISSDGRRTF------DYSD-------KDARKLHMF-- 566
           DFI+++A              C +    R  +      +Y++       ++ + L  F  
Sbjct: 519 DFINELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLP 578

Query: 567 ----------TLGRYVIHPEIANFVYLRVLIVHSYFIYELPNSIAK-LKYLRYIDISDSA 626
                      L + V    +     LRVL +  Y I  LP    K + + R++D+S + 
Sbjct: 579 LSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTE 638

Query: 627 IWKLPESLVLLYNLQTLRLG--RFIKYLPNDLRKLVSLRHLEFYTDPSHQFKQMPQHLGR 686
           + KLP+SL  +YNLQTL L     +K LP D+  L++LR+L+       + +QMP+  GR
Sbjct: 639 LEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLI---GTKLRQMPRRFGR 698

Query: 687 LIQLQTLSSFVVEFHDGYKIEELGFLKNLKGKLSLLCLERVKNKKEAMAAKLVEKEHIFH 746
           L  LQTL++F V   DG +I ELG L +L GKL ++ L+RV +  +A  A L  K+H+  
Sbjct: 699 LKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLRE 758

Query: 747 LDFQW------GWKHERESSSYNDLDVLEGLQPHKNLQALRIQNFAGQLLP---SRIFVE 806
           +DF W         +     + N+ +V E L+PH++++ L I+ + G+  P   S     
Sbjct: 759 IDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFS 818

Query: 807 NLVEIYLQDCERCETLPMLGQLSKLEVLEIRNLPSVRRIGDEFYGN----YCRERTLFPE 866
            +V I L++C+ C +LP LGQL  L+ L I  +  ++ IG +FY +      +++  F  
Sbjct: 819 RIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRS 878

Query: 867 LKTFHIFEMKSLEHWEEIASISNGIMFPHLESLSIICCVKLTNIPNLFASCWKRLEVD-- 909
           L+T     +   + W ++  ++ G +FP L+ L I+ C +LT     F      L +   
Sbjct: 879 LETLRFDNLPDWQEWLDV-RVTRGDLFPSLKKLFILRCPELTGTLPTFLPSLISLHIYKC 938

BLAST of Lsi07G000600 vs. ExPASy TrEMBL
Match: A0A6J1ITZ8 (putative disease resistance protein RGA3 OS=Cucurbita maxima OX=3661 GN=LOC111479330 PE=4 SV=1)

HSP 1 Score: 1462.6 bits (3785), Expect = 0.0e+00
Identity = 746/911 (81.89%), Postives = 809/911 (88.80%), Query Frame = 0

Query: 4   EVLKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLED 63
           E+LKKT  LAA+Q+GL WGFKKDL KMRDSLLM+ AILRDVDRIKS+YP VKPWVEKL+D
Sbjct: 12  ELLKKTASLAAQQLGLAWGFKKDLLKMRDSLLMLHAILRDVDRIKSEYPEVKPWVEKLQD 71

Query: 64  IVFKADVLLDELAYEDLRRKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQ 123
           IVF+ADVLLD+LAYE LRRKVE G+D+   VRNF+S S+NPLVFRLKMANRIKAIS ML+
Sbjct: 72  IVFEADVLLDDLAYERLRRKVETGKDV--MVRNFVSLSKNPLVFRLKMANRIKAISTMLK 131

Query: 124 EHYAAASVAGLVAITSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEM 183
           EHY+AASV GLVAITSKE EPDLSQ+HETDSFLDEFGVIGRDEEV EIVNKLIEL N E+
Sbjct: 132 EHYSAASVVGLVAITSKEIEPDLSQIHETDSFLDEFGVIGRDEEVSEIVNKLIELRNSEV 191

Query: 184 ALSVLPIVGMGGLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPN 243
            LSVLPIVGMGGLGKTA+AK+IFNHEVIR NFDR IWVCVSDPFVINKILRAILETLNPN
Sbjct: 192 -LSVLPIVGMGGLGKTAVAKLIFNHEVIRWNFDRTIWVCVSDPFVINKILRAILETLNPN 251

Query: 244 SGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTS 303
           SGGLESKEALLQEIQK L +KNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVV+VTS
Sbjct: 252 SGGLESKEALLQEIQKWLQEKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVVVTS 311

Query: 304 RSDEVAKIMETHPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPL 363
           RS+EVAKIMETH  H LKKLSNDHCWSLFEKCAFGSD PTIPKLD IR+ELVENF G+PL
Sbjct: 312 RSEEVAKIMETHSWHRLKKLSNDHCWSLFEKCAFGSDSPTIPKLDMIRKELVENFCGIPL 371

Query: 364 VVKVLGGTVKLDKNYERLQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCS 423
           VVKVLGGT+KL KNYERLQS L  L  IPLQDENRVLSTIKLSVDRLPS SLKQCFAYCS
Sbjct: 372 VVKVLGGTMKLYKNYERLQSPLKYLTRIPLQDENRVLSTIKLSVDRLPSPSLKQCFAYCS 431

Query: 424 NFPRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFN-MLSRSLFQDVIKDKRGR 483
           NFP DFEFRKD+LIQMWIAQGFIQPPEE NVTMEDVGD YFN +LSRSLFQDV+KDKRGR
Sbjct: 432 NFPHDFEFRKDSLIQMWIAQGFIQPPEEVNVTMEDVGDNYFNILLSRSLFQDVVKDKRGR 491

Query: 484 TIFCKMHDFIHDVACAISSDGR-RTFDYSDKDARKLHMFTLGRYVIHPEIANFVYLRVLI 543
            ++ KMHDFIHDVA +IS+DGR RT D S++  RKLHM T GR+V H EI NF YLRVLI
Sbjct: 492 AVYFKMHDFIHDVASSISNDGRKRTLDSSNEHLRKLHMLTFGRHVFHSEIGNFTYLRVLI 551

Query: 544 VH-SYFIYELPNSIAKLKYLRYIDISDSAIWKLPESLVLLYNLQTLRLGRFIKYLPNDLR 603
            H SYFI ELPNSI  LK+LRYID+S+S+I KLPESL LLYNLQTL+LGRF++ LP++LR
Sbjct: 552 THSSYFILELPNSIGTLKHLRYIDVSNSSISKLPESLGLLYNLQTLKLGRFVQDLPSNLR 611

Query: 604 KLVSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSSFVVEFHDGYKIEELGFLKNLKGKL 663
            L+ LRHLEFYTDPS+QFKQMP HLGRLIQLQTLS F+V  H+GYKI+ELG LKNLKG L
Sbjct: 612 NLICLRHLEFYTDPSNQFKQMPPHLGRLIQLQTLSCFIVGLHEGYKIDELGSLKNLKGNL 671

Query: 664 SLLCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKHERESSSYNDLDVLEGLQPHKNLQA 723
           SL CLERVKNKKEAMAAKLVEKE IFHL+FQWGWKHERE  +YNDLDVLEGLQPHKNLQA
Sbjct: 672 SLYCLERVKNKKEAMAAKLVEKEKIFHLEFQWGWKHEREGDNYNDLDVLEGLQPHKNLQA 731

Query: 724 LRIQNFAGQLLPSRIFVENLVEIYLQDCERCETLPMLGQLSKLEVLEIRNLPSVRRIGDE 783
           LRI+NFAG+LLPSRIFVEN+VEIYL DCERCETLPMLGQLSKLE+LEIRNLPSVRRIGDE
Sbjct: 732 LRIRNFAGELLPSRIFVENMVEIYLHDCERCETLPMLGQLSKLEILEIRNLPSVRRIGDE 791

Query: 784 FYGNYCRERTLFPELKTFHIFEMKSLEHWEEIASISNGIMFPHLESLSIICCVKLTNIPN 843
           FYGNY RERTLF  LK+FHIFEMK+LEHW EI+ ISN  MFPHLESLS ICC KL NIPN
Sbjct: 792 FYGNYFRERTLFLNLKSFHIFEMKNLEHWGEISCISNDTMFPHLESLSFICCGKLMNIPN 851

Query: 844 LFASCWKRLE-VDVDSASLSSSRGHGKLRSLKILGCEKLTKLWNGLEFCNSLENLWISNC 903
              S  KRLE  DV S +  S RG GKLRSLKILGCE L+KL +GLEFC+S+ENLWISN 
Sbjct: 852 FVVSYCKRLEGDDVTSRTFPSFRGCGKLRSLKILGCENLSKLRDGLEFCSSVENLWISNF 911

Query: 904 SNLNLQNMHSL 911
           S+LNLQNM SL
Sbjct: 912 SDLNLQNMQSL 919

BLAST of Lsi07G000600 vs. ExPASy TrEMBL
Match: A0A6J1CUL2 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014727 PE=4 SV=1)

HSP 1 Score: 1243.4 bits (3216), Expect = 0.0e+00
Identity = 648/938 (69.08%), Postives = 754/938 (80.38%), Query Frame = 0

Query: 4   EVLKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLED 63
           E+LK+ VKLAAEQ  L WGF K+L K+RDSLLMV+AILRDVDRIK ++  VK WVEKL++
Sbjct: 12  ELLKQAVKLAAEQTALAWGFNKELLKLRDSLLMVEAILRDVDRIKGEHQAVKLWVEKLQE 71

Query: 64  IVFKADVLLDELAYEDLRRKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQ 123
           IVF+ADVLLD+LAYE LRRKVE G +    V NF+S S+NPL FRLKMAN+I+AI+RML 
Sbjct: 72  IVFEADVLLDDLAYEHLRRKVEAGNE--AMVSNFVSISKNPLAFRLKMANKIRAIARMLD 131

Query: 124 EHYAAASVAGLVAITSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEM 183
           +HY++A+  GLVA+T KE EPD SQ+ ETDSFLDEFGVIGR+ EV EIVN+LI+ S+ + 
Sbjct: 132 KHYSSAAAVGLVAVTPKEIEPDFSQIQETDSFLDEFGVIGREIEVSEIVNRLIDFSHRD- 191

Query: 184 ALSVLPIVGMGGLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPN 243
           ALSVLPIVGMGGLGKTAL KV+FNHE I+ NFDR IWVCVS+PFVI KILRAILETLNPN
Sbjct: 192 ALSVLPIVGMGGLGKTALMKVVFNHEFIKGNFDRTIWVCVSEPFVIKKILRAILETLNPN 251

Query: 244 SGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTS 303
            GGL+SKEALLQE+QKLL+DK  FLVLDDVWNENL+LWNELKACLLKI+++ GN V+VT+
Sbjct: 252 FGGLDSKEALLQELQKLLNDKRCFLVLDDVWNENLTLWNELKACLLKISQRSGNTVVVTT 311

Query: 304 RSDEVAKIMETHPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPL 363
           RSD+VA++METHPRHHLK LS+DHCWSLF+   FG++   I +LD ++ ELV+ FGG+PL
Sbjct: 312 RSDKVAEVMETHPRHHLKSLSDDHCWSLFKSYVFGNESLDIQELDIVKGELVKRFGGIPL 371

Query: 364 VVKVLGGTVKLDKNYERLQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCS 423
           VVKVLGGTVK D+N E LQS L NLM IPL+ EN VLSTIKLSVDRLPSSSLKQCFAYCS
Sbjct: 372 VVKVLGGTVKFDENSEGLQSTLENLMRIPLEGENHVLSTIKLSVDRLPSSSLKQCFAYCS 431

Query: 424 NFPRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFN-MLSRSLFQDVIKDKRGR 483
           NFP+DFEFRK ALIQMWIAQGFIQ     NVTMEDVGDKYFN +LSRSLFQDV+KD RGR
Sbjct: 432 NFPQDFEFRKQALIQMWIAQGFIQSLGGSNVTMEDVGDKYFNVLLSRSLFQDVVKDSRGR 491

Query: 484 TIFCKMHDFIHDVACAISS------------DG-------------RRTFDYSDKDARKL 543
            I+CKMHD IHDVAC IS+            DG              RT D S+ DARKL
Sbjct: 492 IIYCKMHDLIHDVACVISNAQKLKLNLLDSLDGIRRRGEDRSIGHRIRTLDCSENDARKL 551

Query: 544 HMFTLGRYVIHPEIANFVYLRVLIVHSYFIYELPNSIAKLKYLRYIDISDSAIWKLPESL 603
           H+     +V H +I +FVYLRVLI HS+FI +LP+S+AKLK+LRYIDIS S I +LPES+
Sbjct: 552 HVLAFDSHVFHNKILDFVYLRVLIAHSWFINKLPDSVAKLKHLRYIDISHSTIRELPESI 611

Query: 604 VLLYNLQTLRLGRFIKYLPNDLRKLVSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSSF 663
           VLLYNLQTLRLG F+K LP +LRKLVSLRHLE +T+P +   QMPQ LG+L QLQTLSSF
Sbjct: 612 VLLYNLQTLRLGSFLKDLPKNLRKLVSLRHLEIFTNPCYS-TQMPQRLGQLSQLQTLSSF 671

Query: 664 VVEFHDGYKIEELGFLKNLKGKLSLLCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKHE 723
           VV F +G +IEELG LKNLKGKLSLL LERVK+KKEAM AKLVEK++I  L FQW    E
Sbjct: 672 VVGFDEGCRIEELGTLKNLKGKLSLLRLERVKSKKEAMTAKLVEKDNISDLYFQWCLIGE 731

Query: 724 RESSSYNDLDVLEGLQPHKNLQALRIQNFAGQLLPSRIFVENLVEIYLQDCERCETLPML 783
           R+ S+YNDL+VLEGLQPHKNLQ LRI NFAG+LLP+  FVE LVEIYL  CERC+TLPML
Sbjct: 732 RQGSNYNDLNVLEGLQPHKNLQVLRIHNFAGELLPNGTFVEKLVEIYLHGCERCKTLPML 791

Query: 784 GQLSKLEVLEIRNLPSVRRIGDEFYGNYCRERTLFPELKTFHIFEMKSLEHWEEIASISN 843
           GQLSKLEVLEIR+L SVR IG+EFYGNY  +RTLF +L+TFHI EM SLEHWEEI ++ N
Sbjct: 792 GQLSKLEVLEIRDLHSVRSIGEEFYGNYRDKRTLFHKLRTFHISEMSSLEHWEEIRAVPN 851

Query: 844 GIMFPHLESLSIICCVKLTNIPNLFASCWKRLEVDVDSASLSSSRGHGKLRSLKILGCEK 903
           G +FPHLESLSI+CC++LT+IPN FAS                  G  KLRSLKILGCE 
Sbjct: 852 GTIFPHLESLSIVCCLRLTSIPNFFAS------------HEEERLGSAKLRSLKILGCEN 911

Query: 904 LTKLWNGLEFCNSLENLWISNCSNLN----LQNMHSLT 912
           LT+L NGLEFC+SL+ +WI NCSNLN    LQNMH+L+
Sbjct: 912 LTELPNGLEFCSSLKTMWIGNCSNLNSVPSLQNMHNLS 933

BLAST of Lsi07G000600 vs. ExPASy TrEMBL
Match: A0A0A0KW56 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G015840 PE=4 SV=1)

HSP 1 Score: 1225.3 bits (3169), Expect = 0.0e+00
Identity = 640/939 (68.16%), Postives = 754/939 (80.30%), Query Frame = 0

Query: 4   EVLKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLED 63
           E+LKKTVKLAAEQIGL WGF  +LS +RDSLLMV+AILRDVDRIK+++  VK WVEKLE 
Sbjct: 12  ELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQAVKLWVEKLEA 71

Query: 64  IVFKADVLLDELAYEDLRRKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQ 123
           I+F+ DVLLDELAYEDLRRKVE  +++   V NFIS S+ PLVFRLKMAN+IK I++ML+
Sbjct: 72  IIFEVDVLLDELAYEDLRRKVEPQKEM--MVSNFISFSKTPLVFRLKMANKIKNIAKMLE 131

Query: 124 EHYAAASVAGLVAITSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEM 183
            HY+AAS  GLVAI SK+TEPD SQ+ ETDSFLDE+GVIGR+ EVLEIVN  ++LS  E 
Sbjct: 132 RHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEYGVIGRESEVLEIVNVSVDLSYRE- 191

Query: 184 ALSVLPIVGMGGLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPN 243
            LSVLPIVGMGGLGKTALAKVIFNHE+I+ NFDR +WVCVS+PF+I KILRAILETLN +
Sbjct: 192 NLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRAVWVCVSEPFLIKKILRAILETLNSH 251

Query: 244 SGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTS 303
            GGL+SKEALLQE+QKLL+DK YFLVLDDVWNEN  LWNELK CLLKI+++ GNVV+VT+
Sbjct: 252 FGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQRSGNVVVVTT 311

Query: 304 RSDEVAKIMETHPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPL 363
           RSD VA+IMETH R+HL KLS+DHCWSLF+K AFG++L  IP+LD +++ELV+ FGG+PL
Sbjct: 312 RSDRVAEIMETHSRYHLTKLSDDHCWSLFKKYAFGNELLRIPELDIVQKELVKRFGGIPL 371

Query: 364 VVKVLGGTVKLDKNYERLQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCS 423
            VKV+GG VK D+N+E LQ  L NLM + LQDEN V+STIKL+VDRLP  SLKQCFAYCS
Sbjct: 372 AVKVMGGIVKFDENHEGLQKSLENLMRLQLQDENHVVSTIKLTVDRLPLPSLKQCFAYCS 431

Query: 424 NFPRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFN-MLSRSLFQDVIKDKRGR 483
           NFP+DF+FRK+ALIQMWIAQGFIQP    +  MED+G+KYFN +LSR LFQD++KD RGR
Sbjct: 432 NFPKDFKFRKEALIQMWIAQGFIQPSLGSDEMMEDIGEKYFNVLLSRFLFQDIVKDNRGR 491

Query: 484 TIFCKMHDFIHDVACAISS------------DGR-------------RTFDYSDKDARKL 543
            IFCKMHD IHDVACAIS+            DG              +T D ++  +RKL
Sbjct: 492 IIFCKMHDLIHDVACAISNSPGLKWDPSDLFDGEPWRRQACFASLELKTPDCNENPSRKL 551

Query: 544 HMFTLGRYVIHPEIANFVYLRVLIVHSYFIYELPNSIAKLKYLRYIDISDSAIWKLPESL 603
           HM T   +V H ++ NF+YLRVLI HS+FI +LPNSIAKLK+LRY+DIS S I +LP+S 
Sbjct: 552 HMLTFDSHVFHNKVTNFLYLRVLITHSWFICKLPNSIAKLKHLRYLDISYSTIRELPDSA 611

Query: 604 VLLYNLQTLRLGRFIKYLPNDLRKLVSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSSF 663
           VLLYNLQTL+L RF+  LP +LRKLVSLRHLEF++DP +  KQMPQHLG+LIQLQTLSSF
Sbjct: 612 VLLYNLQTLKLSRFLNGLPKNLRKLVSLRHLEFFSDPCNP-KQMPQHLGKLIQLQTLSSF 671

Query: 664 VVEFHDGYKIEELGFLKNLKGKLSLLCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKHE 723
           VV F DG KIEEL  L+NLKGKLSLLCLERVK+KKEAMAA LVEK +I +L F W  + E
Sbjct: 672 VVGFDDGCKIEELRSLRNLKGKLSLLCLERVKSKKEAMAANLVEKRNISYLSFYWALRCE 731

Query: 724 R-ESSSYNDLDVLEGLQPHKNLQALRIQNFAGQLLPSRIFVENLVEIYLQDCERCETLPM 783
           R E S+YNDL+VLEGLQPHKNLQALRIQNF G+LLP+ IFVENLVEIYL +CE CETLP 
Sbjct: 732 RSEGSNYNDLNVLEGLQPHKNLQALRIQNFLGKLLPNVIFVENLVEIYLHECEMCETLPT 791

Query: 784 LGQLSKLEVLEIRNLPSVRRIGDEFYGNYCRERTLFPELKTFHIFEMKSLEHWEEIASIS 843
           LGQLSKLEVLE+R L SVR IG+EFYGNY  +  LFP LK FHI EM +LE+WEEI  +S
Sbjct: 792 LGQLSKLEVLELRCLYSVRSIGEEFYGNYLEKMILFPTLKAFHICEMINLENWEEIMVVS 851

Query: 844 NGIMFPHLESLSIICCVKLTNIPNLFASCWKRLEVDVDSASLSSSRGHGKLRSLKILGCE 903
           NG +F +LES +I+CC +LT+IPNLFAS           +S  S +   KLRSLKILGCE
Sbjct: 852 NGTIFSNLESFNIVCCPRLTSIPNLFAS--------QHESSFPSLQHSAKLRSLKILGCE 911

Query: 904 KLTKLWNGLEFCNSLENLWISNCSNLN----LQNMHSLT 912
            L K  NGLEFC+SLEN+WISNCSNLN    LQNM +LT
Sbjct: 912 SLQKQPNGLEFCSSLENMWISNCSNLNYPPSLQNMQNLT 938

BLAST of Lsi07G000600 vs. ExPASy TrEMBL
Match: A0A5D3CZ17 (Putative disease resistance protein RGA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001180 PE=4 SV=1)

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 635/927 (68.50%), Postives = 752/927 (81.12%), Query Frame = 0

Query: 4   EVLKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLED 63
           E+LKKTVKLAAEQIGL WGF  +LS +RDSLLMV+AILRDVDRIK+++  VK WVEKLE 
Sbjct: 12  ELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQAVKLWVEKLEA 71

Query: 64  IVFKADVLLDELAYEDLRRKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQ 123
           IVF+ DVLLDELAYEDLRRKVE  ++    V NFIS S+ PLVFRLKMAN+IK I++ML+
Sbjct: 72  IVFEVDVLLDELAYEDLRRKVESQKE--AIVSNFISFSKTPLVFRLKMANKIKNIAKMLE 131

Query: 124 EHYAAASVAGLVAITSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEM 183
           +HY+AAS  GLVAI SK+TEPD SQ+ ETDSFLDE GVIGR+ EVLEIVN  ++LS  E 
Sbjct: 132 KHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEHGVIGRETEVLEIVNVSVDLSYRE- 191

Query: 184 ALSVLPIVGMGGLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPN 243
            LSVLPIVGMGGLGKTALAKVIFNHE+I+ NFDR +WVCVS+PF+I KILRAILETLN N
Sbjct: 192 GLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTVWVCVSEPFLIKKILRAILETLNSN 251

Query: 244 SGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTS 303
            GGL+SKEALLQE+QKLL+DK YFLVLDDVWNEN  LWNELK CLLKI+++ GNVV+VT+
Sbjct: 252 FGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQRSGNVVVVTT 311

Query: 304 RSDEVAKIMETHPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPL 363
           RSD VA+IMETH R+HL KLS+DHCWSLF+K AFG++L  IP+LD +++ELV+ FGG+PL
Sbjct: 312 RSDRVAEIMETHSRYHLTKLSDDHCWSLFKKYAFGNELLGIPELDIVQKELVKRFGGIPL 371

Query: 364 VVKVLGGTVKLDKNYERLQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCS 423
            VKV+GG VK D+N+E LQ  L NLM + LQDEN V+STIKL+VDRLP SSLKQCFAYCS
Sbjct: 372 AVKVMGGIVKFDENHEGLQKSLENLMRLQLQDENHVVSTIKLTVDRLPLSSLKQCFAYCS 431

Query: 424 NFPRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFN-MLSRSLFQDVIKDKRGR 483
           NFP+DF+FR++AL+QMWIAQGFIQP    +  MED+G+KYFN +LSR LFQD++KD RGR
Sbjct: 432 NFPKDFKFRREALVQMWIAQGFIQPSLGSDEMMEDIGEKYFNVLLSRFLFQDIVKDNRGR 491

Query: 484 TIFCKMHDFIHDVACAISS------------DGR-RTFDYSDKDARKLHMFTLGRYVIHP 543
            IFCKMHD IHDVACAIS+            DG  +T D ++  +RKLHM T   +V H 
Sbjct: 492 IIFCKMHDLIHDVACAISNSQGLKWDPSDLLDGELKTPDCNENHSRKLHMLTFDSHVFHN 551

Query: 544 EIANFVYLRVLIVHSYFIYELPNSIAKLKYLRYIDISDSAIWKLPESLVLLYNLQTLRLG 603
           ++ +F++LRVLI HS+FI +LPNSIAKLK+LRY+DIS S I +LP+S+VLLYNLQTL+L 
Sbjct: 552 KVTDFIHLRVLIAHSWFICKLPNSIAKLKHLRYLDISYSTIRELPDSVVLLYNLQTLKLS 611

Query: 604 RFIKYLPNDLRKLVSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSSFVVEFHDGYKIEE 663
           RF   LP +LRKLVSLRHLEF++DP +  KQMPQHLG+LIQLQTLS FVV F DG KIEE
Sbjct: 612 RFFNDLPKNLRKLVSLRHLEFFSDPCNT-KQMPQHLGKLIQLQTLSGFVVGFDDGCKIEE 671

Query: 664 LGFLKNLKGKLSLLCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKHER-ESSSYNDLDV 723
           L  L+NLKG L+LLCLERVK+K EAMAA LVEK +I  L F W  + ER E S+YNDL+V
Sbjct: 672 LRSLRNLKGNLNLLCLERVKSKMEAMAANLVEKGNISFLYFYWTLRSERSEGSNYNDLNV 731

Query: 724 LEGLQPHKNLQALRIQNFAGQLLPSRIFVENLVEIYLQDCERCETLPMLGQLSKLEVLEI 783
           LEGLQPHKNLQALRI+NF G+LLP+ IFVENLVEIYL +CE CETLP LGQLSKLEVLE+
Sbjct: 732 LEGLQPHKNLQALRIRNFLGKLLPNVIFVENLVEIYLHECEICETLPTLGQLSKLEVLEL 791

Query: 784 RNLPSVRRIGDEFYGNYCRERTLFPELKTFHIFEMKSLEHWEEIASISNGIMFPHLESLS 843
           R L SVR IG+EFYGNY  +R LFP LKTFHI EM +LE+WEEI  +SNG +F +LESL+
Sbjct: 792 RCLCSVRSIGEEFYGNYREKRILFPTLKTFHICEMINLENWEEIMVVSNGTIFSNLESLN 851

Query: 844 IICCVKLTNIPNLFASCWKRLEVDVDSASLSSSRGHGKLRSLKILGCEKLTKLWNGLEFC 903
           I+CC +LT+IPNLFA            +S  S +   KLRSLKILGCE L K  NGLEFC
Sbjct: 852 IVCCPRLTSIPNLFAY--------HHESSFPSFQLSAKLRSLKILGCESLQKQPNGLEFC 911

Query: 904 NSLENLWISNCSNLN----LQNMHSLT 912
           +SLEN+WISNCSNLN    L+NM +LT
Sbjct: 912 SSLENMWISNCSNLNYPPSLRNMQNLT 926

BLAST of Lsi07G000600 vs. ExPASy TrEMBL
Match: A0A1S3BXI2 (putative disease resistance protein RGA3 OS=Cucumis melo OX=3656 GN=LOC103494528 PE=4 SV=1)

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 635/927 (68.50%), Postives = 752/927 (81.12%), Query Frame = 0

Query: 4   EVLKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLED 63
           E+LKKTVKLAAEQIGL WGF  +LS +RDSLLMV+AILRDVDRIK+++  VK WVEKLE 
Sbjct: 12  ELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQAVKLWVEKLEA 71

Query: 64  IVFKADVLLDELAYEDLRRKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQ 123
           IVF+ DVLLDELAYEDLRRKVE  ++    V NFIS S+ PLVFRLKMAN+IK I++ML+
Sbjct: 72  IVFEVDVLLDELAYEDLRRKVESQKE--AIVSNFISFSKTPLVFRLKMANKIKNIAKMLE 131

Query: 124 EHYAAASVAGLVAITSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEM 183
           +HY+AAS  GLVAI SK+TEPD SQ+ ETDSFLDE GVIGR+ EVLEIVN  ++LS  E 
Sbjct: 132 KHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEHGVIGRETEVLEIVNVSVDLSYRE- 191

Query: 184 ALSVLPIVGMGGLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPN 243
            LSVLPIVGMGGLGKTALAKVIFNHE+I+ NFDR +WVCVS+PF+I KILRAILETLN N
Sbjct: 192 GLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTVWVCVSEPFLIKKILRAILETLNSN 251

Query: 244 SGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTS 303
            GGL+SKEALLQE+QKLL+DK YFLVLDDVWNEN  LWNELK CLLKI+++ GNVV+VT+
Sbjct: 252 FGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQRSGNVVVVTT 311

Query: 304 RSDEVAKIMETHPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPL 363
           RSD VA+IMETH R+HL KLS+DHCWSLF+K AFG++L  IP+LD +++ELV+ FGG+PL
Sbjct: 312 RSDRVAEIMETHSRYHLTKLSDDHCWSLFKKYAFGNELLGIPELDIVQKELVKRFGGIPL 371

Query: 364 VVKVLGGTVKLDKNYERLQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCS 423
            VKV+GG VK D+N+E LQ  L NLM + LQDEN V+STIKL+VDRLP SSLKQCFAYCS
Sbjct: 372 AVKVMGGIVKFDENHEGLQKSLENLMRLQLQDENHVVSTIKLTVDRLPLSSLKQCFAYCS 431

Query: 424 NFPRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFN-MLSRSLFQDVIKDKRGR 483
           NFP+DF+FR++AL+QMWIAQGFIQP    +  MED+G+KYFN +LSR LFQD++KD RGR
Sbjct: 432 NFPKDFKFRREALVQMWIAQGFIQPSLGSDEMMEDIGEKYFNVLLSRFLFQDIVKDNRGR 491

Query: 484 TIFCKMHDFIHDVACAISS------------DGR-RTFDYSDKDARKLHMFTLGRYVIHP 543
            IFCKMHD IHDVACAIS+            DG  +T D ++  +RKLHM T   +V H 
Sbjct: 492 IIFCKMHDLIHDVACAISNSQGLKWDPSDLLDGELKTPDCNENHSRKLHMLTFDSHVFHN 551

Query: 544 EIANFVYLRVLIVHSYFIYELPNSIAKLKYLRYIDISDSAIWKLPESLVLLYNLQTLRLG 603
           ++ +F++LRVLI HS+FI +LPNSIAKLK+LRY+DIS S I +LP+S+VLLYNLQTL+L 
Sbjct: 552 KVTDFIHLRVLIAHSWFICKLPNSIAKLKHLRYLDISYSTIRELPDSVVLLYNLQTLKLS 611

Query: 604 RFIKYLPNDLRKLVSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSSFVVEFHDGYKIEE 663
           RF   LP +LRKLVSLRHLEF++DP +  KQMPQHLG+LIQLQTLS FVV F DG KIEE
Sbjct: 612 RFFNDLPKNLRKLVSLRHLEFFSDPCNT-KQMPQHLGKLIQLQTLSGFVVGFDDGCKIEE 671

Query: 664 LGFLKNLKGKLSLLCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKHER-ESSSYNDLDV 723
           L  L+NLKG L+LLCLERVK+K EAMAA LVEK +I  L F W  + ER E S+YNDL+V
Sbjct: 672 LRSLRNLKGNLNLLCLERVKSKMEAMAANLVEKGNISFLYFYWTLRSERSEGSNYNDLNV 731

Query: 724 LEGLQPHKNLQALRIQNFAGQLLPSRIFVENLVEIYLQDCERCETLPMLGQLSKLEVLEI 783
           LEGLQPHKNLQALRI+NF G+LLP+ IFVENLVEIYL +CE CETLP LGQLSKLEVLE+
Sbjct: 732 LEGLQPHKNLQALRIRNFLGKLLPNVIFVENLVEIYLHECEICETLPTLGQLSKLEVLEL 791

Query: 784 RNLPSVRRIGDEFYGNYCRERTLFPELKTFHIFEMKSLEHWEEIASISNGIMFPHLESLS 843
           R L SVR IG+EFYGNY  +R LFP LKTFHI EM +LE+WEEI  +SNG +F +LESL+
Sbjct: 792 RCLCSVRSIGEEFYGNYREKRILFPTLKTFHICEMINLENWEEIMVVSNGTIFSNLESLN 851

Query: 844 IICCVKLTNIPNLFASCWKRLEVDVDSASLSSSRGHGKLRSLKILGCEKLTKLWNGLEFC 903
           I+CC +LT+IPNLFA            +S  S +   KLRSLKILGCE L K  NGLEFC
Sbjct: 852 IVCCPRLTSIPNLFAY--------HHESSFPSFQLSAKLRSLKILGCESLQKQPNGLEFC 911

Query: 904 NSLENLWISNCSNLN----LQNMHSLT 912
           +SLEN+WISNCSNLN    L+NM +LT
Sbjct: 912 SSLENMWISNCSNLNYPPSLRNMQNLT 926

BLAST of Lsi07G000600 vs. NCBI nr
Match: XP_038874765.1 (putative disease resistance protein RGA3 [Benincasa hispida])

HSP 1 Score: 1626.7 bits (4211), Expect = 0.0e+00
Identity = 822/910 (90.33%), Postives = 855/910 (93.96%), Query Frame = 0

Query: 4   EVLKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLED 63
           E+LKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKS+YP VKPWVEKLED
Sbjct: 12  EILKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSEYPDVKPWVEKLED 71

Query: 64  IVFKADVLLDELAYEDLRRKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQ 123
           IVF+ADVLLDELAYEDLR KVEIG+D+GKKV NFISASENPL FRLKMANRIKAISRMLQ
Sbjct: 72  IVFEADVLLDELAYEDLRLKVEIGKDLGKKVHNFISASENPLFFRLKMANRIKAISRMLQ 131

Query: 124 EHYAAASVAGLVAITSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEM 183
           EHYAAASV GLVAITSKE EPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEM
Sbjct: 132 EHYAAASVVGLVAITSKEIEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEM 191

Query: 184 ALSVLPIVGMGGLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPN 243
            LSVLPIVGMGGLGKTALAKVIFNHEVI+ENFDR IWVCVSDPFVINKILRAILETLNPN
Sbjct: 192 PLSVLPIVGMGGLGKTALAKVIFNHEVIKENFDRFIWVCVSDPFVINKILRAILETLNPN 251

Query: 244 SGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTS 303
           SGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLK+TRKFGNVV+VTS
Sbjct: 252 SGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKVTRKFGNVVIVTS 311

Query: 304 RSDEVAKIMETHPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPL 363
           RSDEVAKIMETHPRH+LKKLSNDHCW LFEKCAFGSDLPTIPKLDKIRRELVENFGG+PL
Sbjct: 312 RSDEVAKIMETHPRHYLKKLSNDHCWLLFEKCAFGSDLPTIPKLDKIRRELVENFGGIPL 371

Query: 364 VVKVLGGTVKLDKNYERLQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCS 423
           VVKVLGGTVKLDKNYERLQS LTNLM IPLQDENRVLSTIKLSVDRLPS SLKQCFAYCS
Sbjct: 372 VVKVLGGTVKLDKNYERLQSPLTNLMRIPLQDENRVLSTIKLSVDRLPSPSLKQCFAYCS 431

Query: 424 NFPRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFN-MLSRSLFQDVIKDKRGR 483
           NFPRDFEFRKDALIQMWIAQGFIQP EERN+TMEDVGDKYFN +LSRSLFQDV+KDKRGR
Sbjct: 432 NFPRDFEFRKDALIQMWIAQGFIQPSEERNITMEDVGDKYFNILLSRSLFQDVVKDKRGR 491

Query: 484 TIFCKMHDFIHDVACAISSDGRRTFDYSDKDARKLHMFTLGRYVIHPEIANFVYLRVLIV 543
           T+ CKMHDFIHDVACAIS+  RRTFDYSDK ARKLHM T  R V H EIAN +YLRVLI 
Sbjct: 492 TVHCKMHDFIHDVACAISTYRRRTFDYSDKAARKLHMLTFSRRVFHHEIANCIYLRVLIA 551

Query: 544 HSYFIYELPNSIAKLKYLRYIDISDSAIWKLPESLVLLYNLQTLRLGRFIKYLPNDLRKL 603
            SY I ELPNSIA LK+LRYIDIS+S I KLPES+VLLYNLQTLRLGRF++ LPNDLRKL
Sbjct: 552 DSYVICELPNSIAALKHLRYIDISNSLIRKLPESIVLLYNLQTLRLGRFVEDLPNDLRKL 611

Query: 604 VSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSSFVVEFHDGYKIEELGFLKNLKGKLSL 663
           VSLRHLEFY D S+QFKQMP+HLG+LIQLQTLSSFVV  H+GYKIEELGFLKNLKGKLSL
Sbjct: 612 VSLRHLEFYIDRSNQFKQMPKHLGQLIQLQTLSSFVVGLHEGYKIEELGFLKNLKGKLSL 671

Query: 664 LCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKHERESSSYNDLDVLEGLQPHKNLQALR 723
            CLERVKNKKEAMAAKLVEKE+IFHLDFQWGWKH RE SSYNDLDVL+GLQPHKNLQAL 
Sbjct: 672 FCLERVKNKKEAMAAKLVEKENIFHLDFQWGWKHVREGSSYNDLDVLDGLQPHKNLQALT 731

Query: 724 IQNFAGQLLPSRIFVENLVEIYLQDCERCETLPMLGQLSKLEVLEIRNLPSVRRIGDEFY 783
           IQNFAG+LLPSRIFVENLVEIYLQDCERCETLPMLGQL KLEVLEIRNL SVR IGDEFY
Sbjct: 732 IQNFAGELLPSRIFVENLVEIYLQDCERCETLPMLGQLLKLEVLEIRNLHSVRSIGDEFY 791

Query: 784 GNYCRERTLFPELKTFHIFEMKSLEHWEEIASISNGIMFPHLESLSIICCVKLTNIPNLF 843
           GNYC+ERTLFPELKTFHIFEMKSLEHWEEIASISNGIMFPHLESLSII CVKL NIPN+F
Sbjct: 792 GNYCKERTLFPELKTFHIFEMKSLEHWEEIASISNGIMFPHLESLSIISCVKLMNIPNIF 851

Query: 844 ASCWKRLEVDVDSASLSSSRGHGKLRSLKILGCEKLTKL-WNGLEFCNSLENLWISNCSN 903
           AS WKRLEVD DS S SS R HGKLRSLKILGCEKLTKL W  LEFC+SLENLWIS+CSN
Sbjct: 852 ASGWKRLEVDADSVSFSSFRSHGKLRSLKILGCEKLTKLPW--LEFCSSLENLWISSCSN 911

Query: 904 LNLQNMHSLT 912
           LNLQNMHSL+
Sbjct: 912 LNLQNMHSLS 919

BLAST of Lsi07G000600 vs. NCBI nr
Match: XP_023527716.1 (putative disease resistance protein RGA3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1471.8 bits (3809), Expect = 0.0e+00
Identity = 753/911 (82.66%), Postives = 811/911 (89.02%), Query Frame = 0

Query: 4   EVLKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLED 63
           E+LKKTV LAA+QIGL WGFKKDL KMRDSLLM+ AILRDVDRIKS+YP VKPWVEKL+D
Sbjct: 12  ELLKKTVSLAAQQIGLAWGFKKDLLKMRDSLLMLHAILRDVDRIKSEYPEVKPWVEKLQD 71

Query: 64  IVFKADVLLDELAYEDLRRKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQ 123
           IVF+ADVLLD+LAYE LRRKVE G+D    VRNF+S S+NPLVFRLKMANRIKAISRML+
Sbjct: 72  IVFEADVLLDDLAYERLRRKVETGKD--AMVRNFVSLSKNPLVFRLKMANRIKAISRMLK 131

Query: 124 EHYAAASVAGLVAITSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEM 183
           EHY+AASV GLVAITSKE EPDLSQ+HETDSFLDEFGVIGRDEEV EIVNKLIEL N E 
Sbjct: 132 EHYSAASVVGLVAITSKEIEPDLSQIHETDSFLDEFGVIGRDEEVSEIVNKLIELRNSE- 191

Query: 184 ALSVLPIVGMGGLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPN 243
            LSVLPIVGMGGLGKTA+AK+IFNHEVIR NFDR IWVCVSDPFVINKILRAILETLNPN
Sbjct: 192 TLSVLPIVGMGGLGKTAVAKLIFNHEVIRGNFDRTIWVCVSDPFVINKILRAILETLNPN 251

Query: 244 SGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTS 303
           SGGLESKEALLQEIQK L DKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVV+VTS
Sbjct: 252 SGGLESKEALLQEIQKWLQDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVVVTS 311

Query: 304 RSDEVAKIMETHPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPL 363
           RS+EVAKIMETH  H LKKLSNDHCWSLFEKCAFGSD PTIPKLD IR+ELVENF G+PL
Sbjct: 312 RSEEVAKIMETHSWHRLKKLSNDHCWSLFEKCAFGSDSPTIPKLDMIRKELVENFCGIPL 371

Query: 364 VVKVLGGTVKLDKNYERLQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCS 423
           VVKVLGGT+KL KNYERLQS L  L  IPLQDENRVLSTIKLSVDRLPS SLKQCFAYCS
Sbjct: 372 VVKVLGGTMKLYKNYERLQSPLKYLTRIPLQDENRVLSTIKLSVDRLPSPSLKQCFAYCS 431

Query: 424 NFPRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFN-MLSRSLFQDVIKDKRGR 483
           NFP DFEFRKD+LIQMWIAQGFIQPPEE NVTMED+GD YFN +LSRSLFQDV+KDKRGR
Sbjct: 432 NFPHDFEFRKDSLIQMWIAQGFIQPPEEVNVTMEDIGDNYFNILLSRSLFQDVVKDKRGR 491

Query: 484 TIFCKMHDFIHDVACAISSDGRR-TFDYSDKDARKLHMFTLGRYVIHPEIANFVYLRVLI 543
            ++ KMHDFIHDVACAIS+DGR+ T D S++  RKLHM T GR+V H EIANF YLRVLI
Sbjct: 492 AVYFKMHDFIHDVACAISNDGRKTTLDSSNEHPRKLHMLTFGRHVFHSEIANFTYLRVLI 551

Query: 544 VH-SYFIYELPNSIAKLKYLRYIDISDSAIWKLPESLVLLYNLQTLRLGRFIKYLPNDLR 603
            H SYFI ELPNSI  LK+LRYIDIS+S+I KLPESL LLYNLQTL+LGRFI+ LP++LR
Sbjct: 552 THSSYFILELPNSIGSLKHLRYIDISNSSISKLPESLGLLYNLQTLKLGRFIQDLPSNLR 611

Query: 604 KLVSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSSFVVEFHDGYKIEELGFLKNLKGKL 663
            L+ LRHLEFYTDPS+QFKQMPQHLG+LIQLQTLS F+V  H+GYKI+ELG LKNLKG L
Sbjct: 612 NLICLRHLEFYTDPSNQFKQMPQHLGQLIQLQTLSCFIVGLHEGYKIDELGSLKNLKGNL 671

Query: 664 SLLCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKHERESSSYNDLDVLEGLQPHKNLQA 723
           SL CLERVKNKKEAMAAKLVEKE IFHL+FQWGWKHERE  +YNDLDVLEGLQPHKNLQA
Sbjct: 672 SLYCLERVKNKKEAMAAKLVEKEKIFHLEFQWGWKHEREGDNYNDLDVLEGLQPHKNLQA 731

Query: 724 LRIQNFAGQLLPSRIFVENLVEIYLQDCERCETLPMLGQLSKLEVLEIRNLPSVRRIGDE 783
           LRI+NFAG+LLPSRIFVENLVEIYL DCERCETLPMLGQL KLE LEIRNLPSVRRIGDE
Sbjct: 732 LRIRNFAGELLPSRIFVENLVEIYLHDCERCETLPMLGQLPKLEALEIRNLPSVRRIGDE 791

Query: 784 FYGNYCRERTLFPELKTFHIFEMKSLEHWEEIASISNGIMFPHLESLSIICCVKLTNIPN 843
           FYGNY RERTLF  LK+FHIFEMK+LEHW EI+ ISN  MFPHLESLSIICC KL NIPN
Sbjct: 792 FYGNYFRERTLFLNLKSFHIFEMKNLEHWGEISCISNYTMFPHLESLSIICCGKLMNIPN 851

Query: 844 LFASCWKRLE-VDVDSASLSSSRGHGKLRSLKILGCEKLTKLWNGLEFCNSLENLWISNC 903
              S  KRLE  DV S +  S RG GKLRSLKILGCE L+KL +GLEFC+S+ENLW+SN 
Sbjct: 852 FVVSYCKRLEGDDVTSRTFPSFRGCGKLRSLKILGCENLSKLRDGLEFCSSVENLWLSNF 911

Query: 904 SNLNLQNMHSL 911
           S+LNLQNM SL
Sbjct: 912 SDLNLQNMQSL 919

BLAST of Lsi07G000600 vs. NCBI nr
Match: XP_022979680.1 (putative disease resistance protein RGA3 [Cucurbita maxima])

HSP 1 Score: 1462.6 bits (3785), Expect = 0.0e+00
Identity = 746/911 (81.89%), Postives = 809/911 (88.80%), Query Frame = 0

Query: 4   EVLKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLED 63
           E+LKKT  LAA+Q+GL WGFKKDL KMRDSLLM+ AILRDVDRIKS+YP VKPWVEKL+D
Sbjct: 12  ELLKKTASLAAQQLGLAWGFKKDLLKMRDSLLMLHAILRDVDRIKSEYPEVKPWVEKLQD 71

Query: 64  IVFKADVLLDELAYEDLRRKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQ 123
           IVF+ADVLLD+LAYE LRRKVE G+D+   VRNF+S S+NPLVFRLKMANRIKAIS ML+
Sbjct: 72  IVFEADVLLDDLAYERLRRKVETGKDV--MVRNFVSLSKNPLVFRLKMANRIKAISTMLK 131

Query: 124 EHYAAASVAGLVAITSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEM 183
           EHY+AASV GLVAITSKE EPDLSQ+HETDSFLDEFGVIGRDEEV EIVNKLIEL N E+
Sbjct: 132 EHYSAASVVGLVAITSKEIEPDLSQIHETDSFLDEFGVIGRDEEVSEIVNKLIELRNSEV 191

Query: 184 ALSVLPIVGMGGLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPN 243
            LSVLPIVGMGGLGKTA+AK+IFNHEVIR NFDR IWVCVSDPFVINKILRAILETLNPN
Sbjct: 192 -LSVLPIVGMGGLGKTAVAKLIFNHEVIRWNFDRTIWVCVSDPFVINKILRAILETLNPN 251

Query: 244 SGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTS 303
           SGGLESKEALLQEIQK L +KNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVV+VTS
Sbjct: 252 SGGLESKEALLQEIQKWLQEKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVVVTS 311

Query: 304 RSDEVAKIMETHPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPL 363
           RS+EVAKIMETH  H LKKLSNDHCWSLFEKCAFGSD PTIPKLD IR+ELVENF G+PL
Sbjct: 312 RSEEVAKIMETHSWHRLKKLSNDHCWSLFEKCAFGSDSPTIPKLDMIRKELVENFCGIPL 371

Query: 364 VVKVLGGTVKLDKNYERLQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCS 423
           VVKVLGGT+KL KNYERLQS L  L  IPLQDENRVLSTIKLSVDRLPS SLKQCFAYCS
Sbjct: 372 VVKVLGGTMKLYKNYERLQSPLKYLTRIPLQDENRVLSTIKLSVDRLPSPSLKQCFAYCS 431

Query: 424 NFPRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFN-MLSRSLFQDVIKDKRGR 483
           NFP DFEFRKD+LIQMWIAQGFIQPPEE NVTMEDVGD YFN +LSRSLFQDV+KDKRGR
Sbjct: 432 NFPHDFEFRKDSLIQMWIAQGFIQPPEEVNVTMEDVGDNYFNILLSRSLFQDVVKDKRGR 491

Query: 484 TIFCKMHDFIHDVACAISSDGR-RTFDYSDKDARKLHMFTLGRYVIHPEIANFVYLRVLI 543
            ++ KMHDFIHDVA +IS+DGR RT D S++  RKLHM T GR+V H EI NF YLRVLI
Sbjct: 492 AVYFKMHDFIHDVASSISNDGRKRTLDSSNEHLRKLHMLTFGRHVFHSEIGNFTYLRVLI 551

Query: 544 VH-SYFIYELPNSIAKLKYLRYIDISDSAIWKLPESLVLLYNLQTLRLGRFIKYLPNDLR 603
            H SYFI ELPNSI  LK+LRYID+S+S+I KLPESL LLYNLQTL+LGRF++ LP++LR
Sbjct: 552 THSSYFILELPNSIGTLKHLRYIDVSNSSISKLPESLGLLYNLQTLKLGRFVQDLPSNLR 611

Query: 604 KLVSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSSFVVEFHDGYKIEELGFLKNLKGKL 663
            L+ LRHLEFYTDPS+QFKQMP HLGRLIQLQTLS F+V  H+GYKI+ELG LKNLKG L
Sbjct: 612 NLICLRHLEFYTDPSNQFKQMPPHLGRLIQLQTLSCFIVGLHEGYKIDELGSLKNLKGNL 671

Query: 664 SLLCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKHERESSSYNDLDVLEGLQPHKNLQA 723
           SL CLERVKNKKEAMAAKLVEKE IFHL+FQWGWKHERE  +YNDLDVLEGLQPHKNLQA
Sbjct: 672 SLYCLERVKNKKEAMAAKLVEKEKIFHLEFQWGWKHEREGDNYNDLDVLEGLQPHKNLQA 731

Query: 724 LRIQNFAGQLLPSRIFVENLVEIYLQDCERCETLPMLGQLSKLEVLEIRNLPSVRRIGDE 783
           LRI+NFAG+LLPSRIFVEN+VEIYL DCERCETLPMLGQLSKLE+LEIRNLPSVRRIGDE
Sbjct: 732 LRIRNFAGELLPSRIFVENMVEIYLHDCERCETLPMLGQLSKLEILEIRNLPSVRRIGDE 791

Query: 784 FYGNYCRERTLFPELKTFHIFEMKSLEHWEEIASISNGIMFPHLESLSIICCVKLTNIPN 843
           FYGNY RERTLF  LK+FHIFEMK+LEHW EI+ ISN  MFPHLESLS ICC KL NIPN
Sbjct: 792 FYGNYFRERTLFLNLKSFHIFEMKNLEHWGEISCISNDTMFPHLESLSFICCGKLMNIPN 851

Query: 844 LFASCWKRLE-VDVDSASLSSSRGHGKLRSLKILGCEKLTKLWNGLEFCNSLENLWISNC 903
              S  KRLE  DV S +  S RG GKLRSLKILGCE L+KL +GLEFC+S+ENLWISN 
Sbjct: 852 FVVSYCKRLEGDDVTSRTFPSFRGCGKLRSLKILGCENLSKLRDGLEFCSSVENLWISNF 911

Query: 904 SNLNLQNMHSL 911
           S+LNLQNM SL
Sbjct: 912 SDLNLQNMQSL 919

BLAST of Lsi07G000600 vs. NCBI nr
Match: XP_022145224.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 1243.4 bits (3216), Expect = 0.0e+00
Identity = 648/938 (69.08%), Postives = 754/938 (80.38%), Query Frame = 0

Query: 4   EVLKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLED 63
           E+LK+ VKLAAEQ  L WGF K+L K+RDSLLMV+AILRDVDRIK ++  VK WVEKL++
Sbjct: 12  ELLKQAVKLAAEQTALAWGFNKELLKLRDSLLMVEAILRDVDRIKGEHQAVKLWVEKLQE 71

Query: 64  IVFKADVLLDELAYEDLRRKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQ 123
           IVF+ADVLLD+LAYE LRRKVE G +    V NF+S S+NPL FRLKMAN+I+AI+RML 
Sbjct: 72  IVFEADVLLDDLAYEHLRRKVEAGNE--AMVSNFVSISKNPLAFRLKMANKIRAIARMLD 131

Query: 124 EHYAAASVAGLVAITSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEM 183
           +HY++A+  GLVA+T KE EPD SQ+ ETDSFLDEFGVIGR+ EV EIVN+LI+ S+ + 
Sbjct: 132 KHYSSAAAVGLVAVTPKEIEPDFSQIQETDSFLDEFGVIGREIEVSEIVNRLIDFSHRD- 191

Query: 184 ALSVLPIVGMGGLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPN 243
           ALSVLPIVGMGGLGKTAL KV+FNHE I+ NFDR IWVCVS+PFVI KILRAILETLNPN
Sbjct: 192 ALSVLPIVGMGGLGKTALMKVVFNHEFIKGNFDRTIWVCVSEPFVIKKILRAILETLNPN 251

Query: 244 SGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTS 303
            GGL+SKEALLQE+QKLL+DK  FLVLDDVWNENL+LWNELKACLLKI+++ GN V+VT+
Sbjct: 252 FGGLDSKEALLQELQKLLNDKRCFLVLDDVWNENLTLWNELKACLLKISQRSGNTVVVTT 311

Query: 304 RSDEVAKIMETHPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPL 363
           RSD+VA++METHPRHHLK LS+DHCWSLF+   FG++   I +LD ++ ELV+ FGG+PL
Sbjct: 312 RSDKVAEVMETHPRHHLKSLSDDHCWSLFKSYVFGNESLDIQELDIVKGELVKRFGGIPL 371

Query: 364 VVKVLGGTVKLDKNYERLQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCS 423
           VVKVLGGTVK D+N E LQS L NLM IPL+ EN VLSTIKLSVDRLPSSSLKQCFAYCS
Sbjct: 372 VVKVLGGTVKFDENSEGLQSTLENLMRIPLEGENHVLSTIKLSVDRLPSSSLKQCFAYCS 431

Query: 424 NFPRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFN-MLSRSLFQDVIKDKRGR 483
           NFP+DFEFRK ALIQMWIAQGFIQ     NVTMEDVGDKYFN +LSRSLFQDV+KD RGR
Sbjct: 432 NFPQDFEFRKQALIQMWIAQGFIQSLGGSNVTMEDVGDKYFNVLLSRSLFQDVVKDSRGR 491

Query: 484 TIFCKMHDFIHDVACAISS------------DG-------------RRTFDYSDKDARKL 543
            I+CKMHD IHDVAC IS+            DG              RT D S+ DARKL
Sbjct: 492 IIYCKMHDLIHDVACVISNAQKLKLNLLDSLDGIRRRGEDRSIGHRIRTLDCSENDARKL 551

Query: 544 HMFTLGRYVIHPEIANFVYLRVLIVHSYFIYELPNSIAKLKYLRYIDISDSAIWKLPESL 603
           H+     +V H +I +FVYLRVLI HS+FI +LP+S+AKLK+LRYIDIS S I +LPES+
Sbjct: 552 HVLAFDSHVFHNKILDFVYLRVLIAHSWFINKLPDSVAKLKHLRYIDISHSTIRELPESI 611

Query: 604 VLLYNLQTLRLGRFIKYLPNDLRKLVSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSSF 663
           VLLYNLQTLRLG F+K LP +LRKLVSLRHLE +T+P +   QMPQ LG+L QLQTLSSF
Sbjct: 612 VLLYNLQTLRLGSFLKDLPKNLRKLVSLRHLEIFTNPCYS-TQMPQRLGQLSQLQTLSSF 671

Query: 664 VVEFHDGYKIEELGFLKNLKGKLSLLCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKHE 723
           VV F +G +IEELG LKNLKGKLSLL LERVK+KKEAM AKLVEK++I  L FQW    E
Sbjct: 672 VVGFDEGCRIEELGTLKNLKGKLSLLRLERVKSKKEAMTAKLVEKDNISDLYFQWCLIGE 731

Query: 724 RESSSYNDLDVLEGLQPHKNLQALRIQNFAGQLLPSRIFVENLVEIYLQDCERCETLPML 783
           R+ S+YNDL+VLEGLQPHKNLQ LRI NFAG+LLP+  FVE LVEIYL  CERC+TLPML
Sbjct: 732 RQGSNYNDLNVLEGLQPHKNLQVLRIHNFAGELLPNGTFVEKLVEIYLHGCERCKTLPML 791

Query: 784 GQLSKLEVLEIRNLPSVRRIGDEFYGNYCRERTLFPELKTFHIFEMKSLEHWEEIASISN 843
           GQLSKLEVLEIR+L SVR IG+EFYGNY  +RTLF +L+TFHI EM SLEHWEEI ++ N
Sbjct: 792 GQLSKLEVLEIRDLHSVRSIGEEFYGNYRDKRTLFHKLRTFHISEMSSLEHWEEIRAVPN 851

Query: 844 GIMFPHLESLSIICCVKLTNIPNLFASCWKRLEVDVDSASLSSSRGHGKLRSLKILGCEK 903
           G +FPHLESLSI+CC++LT+IPN FAS                  G  KLRSLKILGCE 
Sbjct: 852 GTIFPHLESLSIVCCLRLTSIPNFFAS------------HEEERLGSAKLRSLKILGCEN 911

Query: 904 LTKLWNGLEFCNSLENLWISNCSNLN----LQNMHSLT 912
           LT+L NGLEFC+SL+ +WI NCSNLN    LQNMH+L+
Sbjct: 912 LTELPNGLEFCSSLKTMWIGNCSNLNSVPSLQNMHNLS 933

BLAST of Lsi07G000600 vs. NCBI nr
Match: XP_038900029.1 (putative disease resistance protein RGA3 [Benincasa hispida])

HSP 1 Score: 1231.1 bits (3184), Expect = 0.0e+00
Identity = 649/939 (69.12%), Postives = 755/939 (80.40%), Query Frame = 0

Query: 4   EVLKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLED 63
           E+LKKTVKLAA+QIGL WGFK +LS +RDSLLMV+AILRDVDRIK+++  VK WVEKLED
Sbjct: 12  ELLKKTVKLAAKQIGLAWGFKNELSNLRDSLLMVEAILRDVDRIKAEHQAVKLWVEKLED 71

Query: 64  IVFKADVLLDELAYEDLRRKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQ 123
           IVF+ADVLLDELAYED+RRK E  +++   V NF+S S+NPLVFRLKMAN+IK I+RML+
Sbjct: 72  IVFEADVLLDELAYEDIRRKAEPQKEV--TVSNFVSFSKNPLVFRLKMANKIKNIARMLE 131

Query: 124 EHYAAASVAGLVAITSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEM 183
           +HY+AAS  GLVAI SK+ EPD SQ+ ETDSF DEFGVIGR+ EVLEIV   ++LS  E 
Sbjct: 132 KHYSAASTVGLVAIISKQIEPDFSQIQETDSFPDEFGVIGREAEVLEIVKISVDLSYRE- 191

Query: 184 ALSVLPIVGMGGLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPN 243
           A+SVLPIVGMGGLGKTALAKVIFNHE+I+ NFDR IWVCVS+PF+I KILR ILETLNPN
Sbjct: 192 AVSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTIWVCVSEPFLIKKILRTILETLNPN 251

Query: 244 SGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTS 303
            GGL+SKEALLQE+QKLL+DK YFLVLDDVWNEN  LWNELK+CLLKI+++ GNVV+VT+
Sbjct: 252 FGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKSCLLKISQRSGNVVVVTT 311

Query: 304 RSDEVAKIMETHPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPL 363
           RS  VA+IMETH R+HL KLS+DHCWSLF+K AFG++   IP+LD +++ELV+ FGG+PL
Sbjct: 312 RSGRVAEIMETHSRYHLTKLSDDHCWSLFKKYAFGNEFLGIPELDIVQKELVKRFGGIPL 371

Query: 364 VVKVLGGTVKLDKNYERLQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCS 423
           VVKVLGG VK D+ +E LQ  L NLM I LQDEN V+STIKL+VDRLP SSLKQCFAYCS
Sbjct: 372 VVKVLGGIVKFDETHEGLQQTLENLMKIQLQDENHVVSTIKLTVDRLPLSSLKQCFAYCS 431

Query: 424 NFPRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFN-MLSRSLFQDVIKDKRGR 483
           NFP+DF+FRK+ALIQMWIAQGFIQPPE  +  MED+G+KYFN +LSR LFQD++KD RGR
Sbjct: 432 NFPKDFKFRKEALIQMWIAQGFIQPPEGSDEMMEDIGEKYFNVLLSRLLFQDIVKDNRGR 491

Query: 484 TIFCKMHDFIHDVACAISS------------DGR-------------RTFDYSDKDARKL 543
            +FCKMHD IHDVA AIS+            DG              RT D ++  +RKL
Sbjct: 492 IVFCKMHDLIHDVALAISNSQELILDPLDLLDGEPWRREACSASHKIRTPDCNENHSRKL 551

Query: 544 HMFTLGRYVIHPEIANFVYLRVLIVHSYFIYELPNSIAKLKYLRYIDISDSAIWKLPESL 603
           HM T   +V H +I +F+YLRVLI HS+FI +LPNSIAKLK+LRY+DIS S I +LPESL
Sbjct: 552 HMLTFDSHVFHNKITDFIYLRVLIAHSWFICKLPNSIAKLKHLRYLDISYSTIRELPESL 611

Query: 604 VLLYNLQTLRLGRFIKYLPNDLRKLVSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSSF 663
            LLYNLQTL+L RF+  LP +LRKLVSLRHLEF+ DP    KQMPQHLG+LIQLQTLSSF
Sbjct: 612 FLLYNLQTLKLSRFLNDLPKNLRKLVSLRHLEFFADPC-DTKQMPQHLGKLIQLQTLSSF 671

Query: 664 VVEFHDGYKIEELGFLKNLKGKLSLLCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKHE 723
           VV F DG KIEELG L+NLKGKL+LLCLERVK+KKEAMAA LVEK +I  L F W  + E
Sbjct: 672 VVGFDDGCKIEELGPLRNLKGKLNLLCLERVKSKKEAMAANLVEKGNISFLYFHWTLRCE 731

Query: 724 R-ESSSYNDLDVLEGLQPHKNLQALRIQNFAGQLLPSRIFVENLVEIYLQDCERCETLPM 783
           R E S+YNDL+VLEGLQPHKNLQALRI NF G+LLP+ IFVENLVEIYL DCE CE LPM
Sbjct: 732 RSEGSNYNDLNVLEGLQPHKNLQALRIHNFLGKLLPNGIFVENLVEIYLHDCEICEALPM 791

Query: 784 LGQLSKLEVLEIRNLPSVRRIGDEFYGNYCRERTLFPELKTFHIFEMKSLEHWEEIASIS 843
           LGQLSKLEVLE+R L SV  IG+EFYGNY  +RTLFP+LK FHI EM SLE+WEEI  +S
Sbjct: 792 LGQLSKLEVLELRCLHSVGSIGEEFYGNYREKRTLFPKLKAFHINEMISLENWEEIMVVS 851

Query: 844 NGIMFPHLESLSIICCVKLTNIPNLFASCWKRLEVDVDSASLSSSRGHGKLRSLKILGCE 903
           NG +FPHLESLSI+CC +LTNIPN FAS           +S  S +G  KLRSLKILGCE
Sbjct: 852 NGTIFPHLESLSIVCCPRLTNIPNFFAS--------HHESSFPSFQGSTKLRSLKILGCE 911

Query: 904 KLTKLWNGLEFCNSLENLWISNCSNLN----LQNMHSLT 912
            L KL N LEFC+SLEN+ ISN S LN    LQNM +LT
Sbjct: 912 NLRKLPNDLEFCSSLENMRISNSSKLNSPPRLQNMQNLT 938

BLAST of Lsi07G000600 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 376.7 bits (966), Expect = 5.3e-104
Identity = 284/962 (29.52%), Postives = 489/962 (50.83%), Query Frame = 0

Query: 27  LSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLEDIVFKADVLLDELAYEDLRRKVEI 86
           L ++  +LL + A+L D +  +   P V+ WV +L D+V+ A+  LD++A E LR  +  
Sbjct: 39  LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98

Query: 87  GEDIGKKVR---------NFISASENPLVFRLKMANRIKAISRMLQEHYAAASVAGLVAI 146
                 ++R         +F+  +   L        R++ ++  L+   +  ++ GL  +
Sbjct: 99  ESSSSNRLRQLRGRMSLGDFLDGNSEHL------ETRLEKVTIRLERLASQRNILGLKEL 158

Query: 147 TSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEMALSVLPIVGMGGLG 206
           T+   +  L     T S +DE  V GRD++  EI+  LI  +  +  ++V+ IVG+GG+G
Sbjct: 159 TAMIPKQRL----PTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVG 218

Query: 207 KTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPNSGGLESKEALLQEI 266
           KT L+++++N + +R  F   +W  VS+ F + KI + + E++          + L  ++
Sbjct: 219 KTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKL 278

Query: 267 QKLLHDKN--YFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTSRSDEVAKIMETH 326
           ++ L      + LVLDD+WNEN + W+ L+   +   +  G+ +LVT+RS  VA IM   
Sbjct: 279 KERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQ--GSQILVTTRSQRVASIMCAV 338

Query: 327 PRHHLKKLSNDHCWSLFEKCAFGSDLPTIPK-LDKIRRELVENFGGVPLVVKVLGGTVKL 386
             H+L+ LS+  CWSLF K  FG+  P + + +  +   +V    G+PL VK LGG ++ 
Sbjct: 339 HVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRF 398

Query: 387 DK---NYER-LQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCSNFPRDFE 446
           +     +ER L SR+ +L +    D++ +L  +++S   LP + LK+CFAYCS FP+   
Sbjct: 399 EGKVIEWERVLSSRIWDLPA----DKSNLLPVLRVSYYYLP-AHLKRCFAYCSIFPKGHA 458

Query: 447 FRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFNML-SRSLFQDVIKDKRGRTIFCKMH 506
           F KD ++ +W+A+GF+Q     +  +E++G++YF+ L SRSL Q      + +T +  MH
Sbjct: 459 FEKDKVVLLWMAEGFLQQTRS-SKNLEELGNEYFSELESRSLLQ------KTKTRYI-MH 518

Query: 507 DFIHDVA--------------CAISSDGRRTF------DYSD-------KDARKLHMF-- 566
           DFI+++A              C +    R  +      +Y++       ++ + L  F  
Sbjct: 519 DFINELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLP 578

Query: 567 ----------TLGRYVIHPEIANFVYLRVLIVHSYFIYELPNSIAK-LKYLRYIDISDSA 626
                      L + V    +     LRVL +  Y I  LP    K + + R++D+S + 
Sbjct: 579 LSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTE 638

Query: 627 IWKLPESLVLLYNLQTLRLG--RFIKYLPNDLRKLVSLRHLEFYTDPSHQFKQMPQHLGR 686
           + KLP+SL  +YNLQTL L     +K LP D+  L++LR+L+       + +QMP+  GR
Sbjct: 639 LEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLI---GTKLRQMPRRFGR 698

Query: 687 LIQLQTLSSFVVEFHDGYKIEELGFLKNLKGKLSLLCLERVKNKKEAMAAKLVEKEHIFH 746
           L  LQTL++F V   DG +I ELG L +L GKL ++ L+RV +  +A  A L  K+H+  
Sbjct: 699 LKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLRE 758

Query: 747 LDFQW------GWKHERESSSYNDLDVLEGLQPHKNLQALRIQNFAGQLLP---SRIFVE 806
           +DF W         +     + N+ +V E L+PH++++ L I+ + G+  P   S     
Sbjct: 759 IDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFS 818

Query: 807 NLVEIYLQDCERCETLPMLGQLSKLEVLEIRNLPSVRRIGDEFYGN----YCRERTLFPE 866
            +V I L++C+ C +LP LGQL  L+ L I  +  ++ IG +FY +      +++  F  
Sbjct: 819 RIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRS 878

Query: 867 LKTFHIFEMKSLEHWEEIASISNGIMFPHLESLSIICCVKLTNIPNLFASCWKRLEVD-- 909
           L+T     +   + W ++  ++ G +FP L+ L I+ C +LT     F      L +   
Sbjct: 879 LETLRFDNLPDWQEWLDV-RVTRGDLFPSLKKLFILRCPELTGTLPTFLPSLISLHIYKC 938

BLAST of Lsi07G000600 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 306.2 bits (783), Expect = 8.8e-83
Identity = 251/871 (28.82%), Postives = 420/871 (48.22%), Query Frame = 0

Query: 27  LSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLEDIVFKADVLLDELAYEDLRRKV-- 86
           L +++ +L+    +L D D+       VK W+  ++D  F+A+ +LDEL  E LRR+V  
Sbjct: 36  LKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVA 95

Query: 87  EIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQEHYAAASVAGLVAITSKETEP 146
           E G  +G   +N ++  E     + K+  +++ + R+L+ H     V GL    S+  EP
Sbjct: 96  EAG-GLGGLFQNLMAGRE---AIQKKIEPKMEKVVRLLEHHVKHIEVIGLKEY-SETREP 155

Query: 147 DLSQVHET-DSFLDEFGVIGRDEEVLEIVNKLI---ELSNGEMALSVLPIVGMGGLGKTA 206
              Q   +    L +  ++GR E+ L +VN L+   E+S G+ A  V+ +VGM G+GKT 
Sbjct: 156 QWRQASRSRPDDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPA--VISVVGMPGVGKTT 215

Query: 207 LAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPNSGGLESKEALLQEIQKL 266
           L +++FN   + E+F+  +W+     F +  + +A+L+ +  ++   E   +L  +++K 
Sbjct: 216 LTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKT 275

Query: 267 LHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTSRSDEVAKIMETHPRHHL 326
           L  K + LVLDD W+E+ S W   +          G+ +++T+RS+ V+ + +    + +
Sbjct: 276 LSGKRFLLVLDDFWSESDSEWESFQVAFTDAEE--GSKIVLTTRSEIVSTVAKAEKIYQM 335

Query: 327 KKLSNDHCWSLFEKCAFGS-DLPTI-PKLDKIRRELVENFGGVPLVVKVLGGTVKLDKNY 386
           K ++N+ CW L  + AFG+  + +I  +L+ I + + E   G+PL  + +   ++   N 
Sbjct: 336 KLMTNEECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNP 395

Query: 387 ERLQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCSNFPRDFEFRKDALIQ 446
           +   +   N  S      N +L  +KLS D LP   LK+CFA CS FP+   F ++ L+ 
Sbjct: 396 DDWYAVSKNFSSY----TNSILPVLKLSYDSLP-PQLKRCFALCSIFPKGHVFDREELVL 455

Query: 447 MWIAQGFIQPPEERNVTMEDVGDKYF-NMLSRSLFQDVIKDKRGRTIFCKMHDFIHDVAC 506
           +W+A   +  P      +ED+G+ Y  +++++S FQ +       T F  MHD ++D+A 
Sbjct: 456 LWMAIDLLYQPRSSR-RLEDIGNDYLGDLVAQSFFQRL---DITMTSFV-MHDLMNDLAK 515

Query: 507 AISSD-----------------GRRTFDYSDKDA----------------------RKLH 566
           A+S D                    +F  S  DA                        L 
Sbjct: 516 AVSGDFCFRLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFLRTILPFNSPTSLE 575

Query: 567 MFTLGRYVIHPEIANFVYLRVLIVHSYFIYELPNSIAKLKYLRYIDISDSAIWKLPESLV 626
              L   V++P +     LR+L +  Y I  LP S+  LK LRY+D+S + I +LPE + 
Sbjct: 576 SLQLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDLSSTKIKELPEFVC 635

Query: 627 LLYNLQTLRLG--RFIKYLPNDLRKLVSLRHLEFYTDPSHQFKQMPQHLGRLIQLQTLSS 686
            L NLQTL L   R +  LP  + +L++LR L+    P     +MP  + +L  LQ LS+
Sbjct: 636 TLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTP---LVEMPPGIKKLRSLQKLSN 695

Query: 687 FVVEFHDGYKIEELGFLKNLKGKLSLLCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKH 746
           FV+    G  + EL  L +L+G L +  L+ V    EA  A L  K  +  L  +W  K 
Sbjct: 696 FVIGRLSGAGLHELKELSHLRGTLRISELQNVAFASEAKDAGLKRKPFLDGLILKWTVKG 755

Query: 747 ER-ESSSYNDL-----DVLEGLQPHKNLQALRIQNFAGQLLP-----SRIFVENLVEIYL 806
                 S+N L     +VL  L+PH +L+   I+++ G   P     S  F   +  + L
Sbjct: 756 SGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFF--GITSVTL 815

Query: 807 QDCERCETLPMLGQLSKLEVLEIRNLPSVRRIG-DEFYGNYCRERTLFPELKTFHIFEMK 836
             C  C +LP +GQL  L+ L I     ++++G D F+G        F  L+    + M 
Sbjct: 816 SSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGVPFQSLQILKFYGMP 875

BLAST of Lsi07G000600 vs. TAIR 10
Match: AT1G53350.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 234.6 bits (597), Expect = 3.3e-61
Identity = 237/893 (26.54%), Postives = 407/893 (45.58%), Query Frame = 0

Query: 22  GFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLEDIVFKADVLLDELAYEDLR 81
           G  + +  ++  L  +Q++L+D D  K++   V+ ++E ++DIV+ AD +++     +LR
Sbjct: 26  GIDEQVDGLKRQLGRLQSLLKDADAKKNETERVRNFLEDVKDIVYDADDIIESFLLNELR 85

Query: 82  RKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQEHYAAASVAGLVAIT--- 141
            K    + I K+VR         LV R K A+ I+ I++ + E        G+  I    
Sbjct: 86  GK---EKGIKKQVRTLACF----LVDRRKFASDIEGITKRISEVIVGMQSLGIQHIADGG 145

Query: 142 --SKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEMALSVLPIVGMGGL 201
             S   +    ++ +T S   E  ++G D+ V E+V+ L+E      ++ V+ + GMGG+
Sbjct: 146 GRSLSLQERQREIRQTFSRNSESDLVGLDQSVEELVDHLVE----NDSVQVVSVSGMGGI 205

Query: 202 GKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNP-NSGGLESKEALLQ 261
           GKT LA+ +F+H+++R +FD   WVCVS  F    + + IL+ L P + G ++  E  LQ
Sbjct: 206 GKTTLARQVFHHDIVRRHFDGFSWVCVSQQFTRKDVWQRILQDLRPYDEGIIQMDEYTLQ 265

Query: 262 -EIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTSRSDEVAKIME- 321
            E+ +LL    Y LVLDDVW E    W+ +KA       K G  +L+TSR++ +    + 
Sbjct: 266 GELFELLESGRYLLVLDDVWKE--EDWDRIKAV---FPHKRGWKMLLTSRNEGLGLHADP 325

Query: 322 THPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDK-IRRELVENFGGVPLVVKVLGG-- 381
           T      + L+ +  W LFE+        T  K+D+ + +E+V   GG+PL VKVLGG  
Sbjct: 326 TCFAFRPRILTPEQSWKLFERIVSSRRDKTEFKVDEAMGKEMVTYCGGLPLAVKVLGGLL 385

Query: 382 -----TVKLDKNYERLQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCSNF 441
                 ++  + +  + + +     +   + N V   + LS + LP   LK CF Y ++F
Sbjct: 386 AKKHTVLEWKRVHSNIVTHIVGKSGLSDDNSNSVYRVLSLSYEDLP-MQLKHCFFYLAHF 445

Query: 442 PRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFNMLSRSLFQDVIKD-KRGRTI 501
           P D++     L   W+A+G I P  + + T++D G+ Y   L R     V +     R  
Sbjct: 446 PEDYKIDVKILFNYWVAEGIITPFHDGS-TIQDTGESYLEELVRRNMVVVEESYLTSRIE 505

Query: 502 FCKMHDFIHDVACAISSD------------------------GRRTFDYS---------- 561
           +C+MHD + +V  + + +                         RR   +S          
Sbjct: 506 YCQMHDMMREVCLSKAKEENFIRVVKVPTTTSTTINAQSPCRSRRLVLHSGNALHMLGHK 565

Query: 562 -DKDARKLHMFTLGRYVIHPEIANFVYLRVLIVHSYFIYE---LPNSIAKLKYLRYIDIS 621
            +K AR + +F +      P     + L  ++  SY  +E   LP+SI  L +LR++ + 
Sbjct: 566 DNKKARSVLIFGVEEKFWKPRGFQCLPLLRVLDLSYVQFEGGKLPSSIGDLIHLRFLSLY 625

Query: 622 DSAIWKLPESLVLLYNLQTLRLG---RFIKYLPNDLRKLVSLRHLEFYTDPSHQFKQMPQ 681
           ++ +  LP SL  L  L  L LG   R + ++PN L+++  LR+L     P     +   
Sbjct: 626 EAGVSHLPSSLGNLKLLLCLNLGVADRLLVHVPNVLKEMQELRYLRL---PRSMPAKTKL 685

Query: 682 HLGRLIQLQTLSSFVVEFHDGYKIEELGFLKNLKGKLSLLCLERVKNKKEAMAAKLVEKE 741
            LG L+ L++L++F  +      +  +  L  L    S  C        E +   L E  
Sbjct: 686 ELGDLVNLESLTNFSTKHGSVTDLLRMTKLSVLNVIFSGEC------TFETLLLSLRELR 745

Query: 742 HIFHLDFQWGWKHERESSSYNDLDVLEGLQPHKNLQALRIQNFAGQLLPSRIFVENLVEI 801
           ++  L F   ++    ++   +L VL+ +       ++ +  F  Q      F  +L  I
Sbjct: 746 NLETLSFH-DFQKVSVANHGGELLVLDFIHLKDLTLSMHLPRFPDQYR----FPPHLAHI 805

Query: 802 YLQDCERCETLPMLGQLSKLEVLE-IRNLPSVRRIGDEFYG-NYCRERTLFPELKTFHIF 853
           +L  C R E  PM        +LE + +L SV      F G      +  FP+L    + 
Sbjct: 806 WLIGC-RMEEDPM-------PILEKLLHLKSVYLSSGAFLGRRMVCSKGGFPQLLALKMS 865

BLAST of Lsi07G000600 vs. TAIR 10
Match: AT1G58807.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 217.6 bits (553), Expect = 4.1e-56
Identity = 248/993 (24.97%), Postives = 441/993 (44.41%), Query Frame = 0

Query: 12  LAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLEDIVFKADVL 71
           L +++  L  G +  +++++  L M+ + L+D +  K     VK  VE++++I++  +  
Sbjct: 16  LLSQECELFQGVEDQVTELKRDLNMLSSFLKDANAKKHTSAVVKNCVEEIKEIIYDGEDT 75

Query: 72  LDELAYEDLRRKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQEHYAAASV 131
           ++    E    K      I K +R       +   + L +      IS+++++  +    
Sbjct: 76  IETFVLEQNLGKT---SGIKKSIRRLACIIPDRRRYALGIGGLSNRISKVIRDMQSFGVQ 135

Query: 132 AGLVAITSKETEPDLS-QVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEMALSVLPI 191
             +V    K+ + D   ++ +  S  D+   +G +  V ++V  L++ +N    + V+ I
Sbjct: 136 QAIVDGGYKQPQGDKQREMRQKFSKDDDSDFVGLEANVKKLVGYLVDEAN----VQVVSI 195

Query: 192 VGMGGLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPNSGGLE-- 251
            GMGGLGKT LAK +FNHE ++  FD + WVCVS  F    + + IL  L P     +  
Sbjct: 196 TGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQKILRDLKPKEEEKKIM 255

Query: 252 --SKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTSRSD 311
             +++ L  E+ +LL      +VLDD+W +    W  +K  +   T+  G  VL+TSR++
Sbjct: 256 EMTQDTLQGELIRLLETSKSLIVLDDIWEK--EDWELIKP-IFPPTK--GWKVLLTSRNE 315

Query: 312 EVAKIMET-HPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRREL----VENFGGV 371
            VA    T +     + L+ +  W+LF++ A         K+D+ + EL    +++ GG+
Sbjct: 316 SVAMRRNTSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKIDEEKEELGKLMIKHCGGL 375

Query: 372 PLVVKVLGGTVK---LDKNYERLQSRLTNLM-----SIPLQDENRVLSTIKLSVDRLPSS 431
           PL ++VLGG +       ++ RL   + + +     +    + N   + + LS + LP S
Sbjct: 376 PLAIRVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNTCNNVLSLSFEELP-S 435

Query: 432 SLKQCFAYCSNFPRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFNMLSRSLFQ 491
            LK CF Y ++FP D+E + + L   W A+G  QP      T+ DVGD Y   L R    
Sbjct: 436 YLKHCFLYLAHFPEDYEIKVENLSYYWAAEGIFQPRHYDGETIRDVGDVYIEELVRRNMV 495

Query: 492 DVIKD-KRGRTIFCKMHDFIHDV--------------------ACAISSDGRRTFDYS-- 551
              +D K  R   C +HD + +V                    A   S+   R F Y   
Sbjct: 496 ISERDVKTSRFETCHLHDMMREVCLLKAKEENFLQITSSRPSTANLQSTVTSRRFVYQYP 555

Query: 552 -----DKD-----ARKLHMFTLGRY-VIHPEIANFVYLRVLIVHSYFIY--ELPNSIAKL 611
                +KD      R L + TLG + +          LRVL +    I   +L + I KL
Sbjct: 556 TTLHVEKDINNPKLRALVVVTLGSWNLAGSSFTRLELLRVLDLIEVKIKGGKLASCIGKL 615

Query: 612 KYLRYIDISDSAIWKLPESLVLLYNLQTLRLGRFIK--YLPNDLRKLVSLRHLEFYTDPS 671
            +LRY+ +  + +  +P SL  L  L  L L  F +  ++PN L  +  LR+L     PS
Sbjct: 616 IHLRYLSLEYAEVTHIPYSLGNLKLLIYLNLASFGRSTFVPNVLMGMQELRYLAL---PS 675

Query: 672 HQFKQMPQHLGRLIQLQTLSSFVVEFHDGYKIEELGFLKNLKGKLSLLCLERVKNKKEAM 731
              ++    L  L++L+TL +F  E      +E+L  +  L   L++  +E  +   E +
Sbjct: 676 DMGRKTKLELSNLVKLETLENFSTE---NSSLEDLCGMVRL-STLNIKLIE--ETSLETL 735

Query: 732 AAKLVEKEHIFHLDFQWGWKHERESSSYNDLDVLEGLQPHKNLQALRIQNFAGQLLPSRI 791
           AA +   +++  L+    + H  E  +     V + +    +L+ L ++ +  +L   + 
Sbjct: 736 AASIGGLKYLEKLEI---YDHGSEMRTKEAGIVFDFV----HLKRLWLKLYMPRLSTEQH 795

Query: 792 FVENLVEIYLQDCERCE-TLPMLGQLSKLEVLEIRNLPSVRRIGDEFYGN--YCRERTLF 851
           F  +L  +YL+ C   E  +P+L +L +L+ LE+          + F G    C     F
Sbjct: 796 FPSHLTTLYLESCRLEEDPMPILEKLLQLKELELG--------FESFSGKKMVCSSGG-F 855

Query: 852 PELKTFHIFEMKSLEHWE------------------EIASISNGIMFPHLESLSI-ICCV 898
           P+L+   + +++  E W+                  ++  + +  +  HL S+S+  CC+
Sbjct: 856 PQLQRLSLLKLEEWEDWKVEESSMPLLRTLDIQVCRKLKQLPDEHLPSHLTSISLFFCCL 915

BLAST of Lsi07G000600 vs. TAIR 10
Match: AT5G35450.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 217.6 bits (553), Expect = 4.1e-56
Identity = 228/891 (25.59%), Postives = 397/891 (44.56%), Query Frame = 0

Query: 22  GFKKDLSKMRDSLLMVQAILRDVDRIKSDYPGVKPWVEKLEDIVFKADVLLDELAYEDLR 81
           G  + +  ++  L  +Q++L+D D  K     V+ ++E ++D+VF A+ +++      LR
Sbjct: 26  GIDEQVDGLKRQLRGLQSLLKDADAKKHGSDRVRNFLEDVKDLVFDAEDIIESYVLNKLR 85

Query: 82  RKVEIGEDIGKKVRNFISASENPLVFRLKMANRIKAISRMLQEHYAAASVAGLV------ 141
                GE  GK V+N +      L  R K+A+ I+ I++ + +        G+       
Sbjct: 86  -----GE--GKGVKNHVRRLACFLTDRHKVASDIEGITKRISKVIGEMQSLGIQQQIIDG 145

Query: 142 --AITSKETEPDLSQVHETDSFLDEFGVIGRDEEVLEIVNKLIELSNGEMALSVLPIVGM 201
             +++ ++ + ++ Q     S   E  ++G ++ V E+V  ++E+ N    + V+ I GM
Sbjct: 146 GRSLSLQDIQREIRQTFPNSS---ESDLVGVEQSVEELVGPMVEIDN----IQVVSISGM 205

Query: 202 GGLGKTALAKVIFNHEVIRENFDRVIWVCVSDPFVINKILRAILETLNPNSGG-LESKEA 261
           GG+GKT LA+ IF+H+++R +FD   WVCVS  F    + + IL+ L P+ G  L+  E 
Sbjct: 206 GGIGKTTLARQIFHHDLVRRHFDGFAWVCVSQQFTQKHVWQRILQELRPHDGEILQMDEY 265

Query: 262 LLQ-EIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKITRKFGNVVLVTSRSDEVAKI 321
            +Q ++ +LL    Y +VLDDVW E    W+ +K       RK G  +L+TSR++ V   
Sbjct: 266 TIQGKLFQLLETGRYLVVLDDVWKE--EDWDRIKEV---FPRKRGWKMLLTSRNEGVGLH 325

Query: 322 ME-THPRHHLKKLSNDHCWSLFEKCAFGSDLPTIPKLDKIRRELVENFGGVPLVVKVLGG 381
            + T      + L+    W LFE+     +     +++ I +E+V   GG+PL VKVLGG
Sbjct: 326 ADPTCLSFRARILNPKESWKLFERIVPRRNETEYEEMEAIGKEMVTYCGGLPLAVKVLGG 385

Query: 382 TV-------KLDKNYERLQSRLTNLMSIPLQDENRVLSTIKLSVDRLPSSSLKQCFAYCS 441
            +       +  +  E + +++     +     N V   + LS + LP + LK CF Y +
Sbjct: 386 LLANKHTASEWKRVSENIGAQIVGKSCLDDNSLNSVYRILSLSYEDLP-TDLKHCFLYLA 445

Query: 442 NFPRDFEFRKDALIQMWIAQGFIQPPEERNVTMEDVGDKYFNMLSRSLFQDVIKDKRG-- 501
           +FP D++ +   L   W A+G         +T+ D G+ Y   L R     VI +K    
Sbjct: 446 HFPEDYKIKTRTLYSYWAAEGIYD-----GLTILDSGEDYLEELVRRNL--VIAEKSNLS 505

Query: 502 -RTIFCKMHDFIHDVACA------------ISSDGRRTFDYSDKDARKLHMFTLGRYVIH 561
            R   C+MHD + +V  +            + +        S   +R+L + +   + I 
Sbjct: 506 WRLKLCQMHDMMREVCISKAKVENFLQIIKVPTSTSTIIAQSPSRSRRLTVHSGKAFHIL 565

Query: 562 PEIANFVYLRVLIVHSYFIYELPNSIAKLKYLRYIDISDSAI--WKLPESLVLLYNLQTL 621
                   L VL +      +  +    L  LR +D+S       KLP S+  L +L+ L
Sbjct: 566 GHKKKVRSLLVLGLKEDLWIQSASRFQSLPLLRVLDLSSVKFEGGKLPSSIGGLIHLRFL 625

Query: 622 RLGR-FIKYLPNDLRKLVSLRHLEFYTD---PSHQFKQMPQHLGRLIQLQTLSSFVVEFH 681
            L +  + +LP+ +R L  + +L  +     P H    +P  L  +++L+ L S  ++ H
Sbjct: 626 SLHQAVVSHLPSTIRNLKLMLYLNLHVAIGVPVH----VPNVLKEMLELRYL-SLPLDMH 685

Query: 682 DGYKIEELGFLKNLKGKLSLLCLERVKNKKEAMAAKLVEKEHIFHLDFQWGWKHERESSS 741
           D  K+ ELG L NL+    L C     +    +    + K   F + F      E  SSS
Sbjct: 686 DKTKL-ELGDLVNLE---YLWCFSTQHSSVTDLLR--MTKLRFFGVSFSERCTFENLSSS 745

Query: 742 ---YNDLDVLEGLQPHK---------------NLQALRIQNFAGQLLPSRIFVENLVEIY 801
              +  L+ L  +   K               +L+ L +     ++        ++  IY
Sbjct: 746 LRQFRKLETLSFIYSRKTYMVDYVGEFVLDFIHLKKLSLGVHLSKIPDQHQLPPHIAHIY 805

Query: 802 LQDCERCE-TLPMLGQLSKLEVLEIRNLPSVRRIGDEFYGNYCRERTLFPELKTFHIFEM 853
           L  C   E  +P+L +L  L+ +E+R    + R            +  FP+L+   I E 
Sbjct: 806 LLFCHMEEDPMPILEKLLHLKSVELRRKAFIGR-------RMVCSKGGFPQLRALQISEQ 865