Lsi07G000450 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi07G000450
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Ribonuclease
Location	chr07: 502947 .. 506686 (+)
RNA-Seq Expression	Lsi07G000450
Synteny	Lsi07G000450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAAGCTACGAAAACAATCAGAACAAAAGCAGCTTCCATGGCGGGAACGACCTAAAAGAAGAGAACACACAATCGAAGAAACGCTCAAGTAAAGAGAGCAAGAGAGATTTTCTTGTAAACTAACGCAGCAAGTAGAAATCAGCAATGTCTTCGCAATATCCACTCCCAAAATGGGCTTCCAAGCCTTGTGTAATGGGCATCGATGAAGCTGGTCGAGGCCCTGTTTTAGGTTTTCTATATTCCTATTCGTTTCGTTCCACTTCTGCCTCTTTAATTCCGCTTCGATCATCATTTTTTGGGACATGATTTTGAATACACTTTTCTTTCTGAAATATTACTGCATTTGATCTCAGGGCCAATGGTGTATGGATGCTTGTACTGCGCTACTTCCTATTTGAAAGAACTTTCGTCTTTGAATTTTGCAGGTTTAGCTTGTTTGCAATTTTCCCGCTTCTATCGATTTTCTTTTAACTGTTGTCCATCATTTCCCTATTTCGTTTACTTTTCTTCCAACTTTTGATGTTTTGCATGTAGACTCAAAAACTTTGAAAGAAGAGAAGAGGGAGGAATTGTTTGAAGATTTGAAGGCTAACGAGTCAATTGGCTGGGCTGTTGATGTCATAGATCCTCGAGAACTTTCATCCAAAATGCTGAACAAGTCAGTCTTTAGTGTTTTATAAATTCTTCCGTTTGCATAAATACTCTAATTTTCACTTTACCTATTCAGTTCGACGGTATAGAGAATTGAACTTAGCTGAAATCTCGTCTTTCTTGAGAAATAGTACTACTTAAGTTGATAATGGGTAAACTTTATATATATTTGTACATGAATTGGTCTTATTAATTCACCACTAGACCCAAAAACTCAAAAACTTGAGTTGATTGATTAAGATAAATGTTGTATTTTTTATATTTATTTTAACTAATGGAAGGTAAGGGAACCTATCTTCATTCAGAGTTAGGAAGAGTCAGCAAAAGACTGGAGCATGGATAATGATGCTTTCCCTCCCCCTTCTATTTTTGTTAACTTATGCACATCAAAGGGTGGGTGACTATGGCTTGGACTAAAGTATTGTTTTTTCTTACTTTATTGGTGAACAGAGTAAAGATAAACCTAAATGAAATATCACATGACTCTGCAATGGGTCTGGTCACCAAGGTTCTAGGCATGGGAGTTCTTTTAACTGAGGTAACTCTCTCCCTGGTTTCTTCATTGATTGATGAAAAGAAACTCAAATTTTTAAGGATACAGTCTCCCAATAGAGAAAGCAGATGAAACTAACAAGGGAAGTCCAATGGATGGCCTCTGGTAACTTCCAGTTATCTACGATCATGATTCTATTGATATCATTTGGAGAAGAGAGAGCATGTGGAATCTTGAACTTCATATGTTATCATTTCATAAGTTCTTTTGGGAGGGAAAAATATAAATTTAAAAAAAGAAATCAAACTACAGATTATTTTCGATGCCTTTCTCTTGTTAATTATAGATCTACACCAAAGTCAACGAGAAGGTAAATGAACCAGTTTTATATCTTGATTACAGTAGCAGGGTGTGACACATTCTTTTTGAGGAAGATCTGTAAGAATTAAATTCTGGAAAGCTATATTCTCGATCTTTTCAAGTCTATAAGCTGGAGGGAAAGAAAATCCGCATTCAGCATATCTCATCTTTCATTGCCTTAAAGTTTTTTTTGTTATCCATTGATGATTTGAAGTCATTTGCAGATTTGTTCTGCCCATTCTTCTTTCAGACTCCATGCATCCAACCTGCCCCATGAACGATGTTAGATTAATCACTTCCATTTATTTTCAGGTTTATGTGGATACTGTTGGAGATCCTGAAAAGTACAGAATTAAGTTGTCTGAAAAATTTCCCAATGTAAAATTTGTAGTTGCAAAGAAAGCTGATAGTTTATATCCCGTTGTCAGTGGGGCAAGCATAGTCGCAAAGGTAATGCTAATGCTTCCTTTGAAGATATAGCATTCCAACTGTTTCCCACATTTAAATTCATCAATTTGACTCCAAAAAAATAAAAATCATCAATTTGACTTGTAGGTCACAAGAGATCGTGCATTGCGGGAATGGGCATTGGAGGAAACAGCTGAAAACATGCATAGAAACTTTGGTTCTGGATACCCTGGAGGTCAATTTCATCTGCTAAATATTTTTTTGCAGCATACTATAATCAGGTTTTCATTGCACAGGGTAAAATCAATAATGCTTTTATGATTTAATCTATATAGATCCAACAACTAAATGTTGGCTAGAAGATCACAAGCACTCGGTGTTTGGGTTCCCCACATTAGTCCGCTTCAGTTGGGGTACCTGCAGTTCATATTTCAAAGACGTTGTTGAAGTATCATGGTTAGTTCTTACTCTGCCTTGTAAGAATTGTAGTTCTTTGCAGAATTAGCTCATTAAGCTATATACAATTTCTTTTATGTTCTCCACTGTATAGAAAGGACAGGCTTTTGTTCTGATCTGTTTGCCTCATGATACCATTATGTATGATGGACGTGGTGTAAATTCTCTTATCCTTCATATTTTTGTGATTATAGCCTTCCATCTCTTATAGCACATTGGAATTGTTTTTGTAAATCATTTGTCATGTTTTGTCATATTTTGTAATTTTATCTTATCAATGTAATGAAGGATAATTTTTTCATTTAAAAAAAAAGATGGACAAATTTCATACTTTTCAGAAATATTTTCACACAATCAAATTATCAGTAATTTAGTTAAAAAAACAGACCACTGTGTTATTAAAAGTTCAAGTATCCTTGTCCAAAATATTGTTAGTTGTCACAACATCCATATAGCACTAGGTATGGTGTGATAAGTGCTTTAGTTGTTGGTGGTGGGATTGATTTAATGAAAGCACTGTCTTAAAATATGTTTGAATATCATATGGAATTTAGTAATTAATGATAGACACTTTTTTTTTTATTAATGTAGTGCACTTCTCATTTTTGCTGAAACATCATCCTGCTCTGATGCGTTGGGATGTTAAGATCTTTCCCATTCTAAATTTTAACTTGATTTGTTCATTGGAAATGAATGGTTGTACCTTTATTCTAGTCTATAGTTATTGTACAATAGTTGGACTTCCAAACAGTTTGGGTTCGTTCCTGATGTCTACTTCTTGATACACTTAGGTTCATTTTGTTGTGTTCGGGTTTATTTACCTACACATTGACTGATCTCATGGGACAATTTTCTCTTTCTAAACATTTCATTGTTAAGAAACTCACAAGATTGTTGTATTCTAAGTAGGTGGCCCTGGCATTCATAACATTGAAACCTATCTTTCAAAATCCTGTGCCACATGGTTACGCGTAGTTTATTTTCATCAATTTCGCTTCTTGTAAATCATCAAATATCATAATCTGATTTATTTGTTTTCCTGTAAGCTGACAATTAGATGCCAAAACTTTTGATACTTCCCTGACAGTCTCACAAACTCAAATTTGACTTTGTTATTAATTTCTTTTGATTTATTCACAGCGATGTCATATTATTATTATTATTATTATTATTATTGTTTATATGCTTGATTTACAGGGAGTCGGATAAAGTGGATGAAGGTGGGGCAACGTCTGGAAGTGGTAAGAAACAATTAAGACTAAGGAATGTTGGTATCACCACATCCAAGAGAAAGAGTGAAGAAATTGAATCAAATGGCAAAGGACGATGCAAGTTTTTCCAGGCTAGAAAACTTGAACAACTCACTCATTTT

mRNA sequence

GGAAGCTACGAAAACAATCAGAACAAAAGCAGCTTCCATGGCGGGAACGACCTAAAAGAAGAGAACACACAATCGAAGAAACGCTCAAGTAAAGAGAGCAAGAGAGATTTTCTTAAATCAGCAATGTCTTCGCAATATCCACTCCCAAAATGGGCTTCCAAGCCTTGTGTAATGGGCATCGATGAAGCTGGTCGAGGCCCTGTTTTAGGGCCAATGGTGTATGGATGCTTGTACTGCGCTACTTCCTATTTGAAAGAACTTTCGTCTTTGAATTTTGCAGACTCAAAAACTTTGAAAGAAGAGAAGAGGGAGGAATTGTTTGAAGATTTGAAGGCTAACGAGTCAATTGGCTGGGCTGTTGATGTCATAGATCCTCGAGAACTTTCATCCAAAATGCTGAACAAAGTAAAGATAAACCTAAATGAAATATCACATGACTCTGCAATGGGTCTGGTCACCAAGGTTCTAGGCATGGGAGTTCTTTTAACTGAGATCTACACCAAAGTCAACGAGAAGGTTTATGTGGATACTGTTGGAGATCCTGAAAAGTACAGAATTAAGTTGTCTGAAAAATTTCCCAATGTAAAATTTGTAGTTGCAAAGAAAGCTGATAGTTTATATCCCGTTGTCAGTGGGGCAAGCATAGTCGCAAAGGTCACAAGAGATCGTGCATTGCGGGAATGGGCATTGGAGGAAACAGCTGAAAACATGCATAGAAACTTTGGTTCTGGATACCCTGGAGCATACTATAATCAGGTTTTCATTGCACAGGATCCAACAACTAAATGTTGGCTAGAAGATCACAAGCACTCGGTGTTTGGGTTCCCCACATTAGTCCGCTTCAGTTGGGGTACCTGCAGTTCATATTTCAAAGACGTTGTTGAAGTGGCCCTGGCATTCATAACATTGAAACCTATCTTTCAAAATCCTGTGCCACATGGTTACGCGGAGTCGGATAAAGTGGATGAAGGTGGGGCAACGTCTGGAAGTGGTAAGAAACAATTAAGACTAAGGAATGTTGGTATCACCACATCCAAGAGAAAGAGTGAAGAAATTGAATCAAATGGCAAAGGACGATGCAAGTTTTTCCAGGCTAGAAAACTTGAACAACTCACTCATTTT

Coding sequence (CDS)

GGAAGCTACGAAAACAATCAGAACAAAAGCAGCTTCCATGGCGGGAACGACCTAAAAGAAGAGAACACACAATCGAAGAAACGCTCAAGTAAAGAGAGCAAGAGAGATTTTCTTAAATCAGCAATGTCTTCGCAATATCCACTCCCAAAATGGGCTTCCAAGCCTTGTGTAATGGGCATCGATGAAGCTGGTCGAGGCCCTGTTTTAGGGCCAATGGTGTATGGATGCTTGTACTGCGCTACTTCCTATTTGAAAGAACTTTCGTCTTTGAATTTTGCAGACTCAAAAACTTTGAAAGAAGAGAAGAGGGAGGAATTGTTTGAAGATTTGAAGGCTAACGAGTCAATTGGCTGGGCTGTTGATGTCATAGATCCTCGAGAACTTTCATCCAAAATGCTGAACAAAGTAAAGATAAACCTAAATGAAATATCACATGACTCTGCAATGGGTCTGGTCACCAAGGTTCTAGGCATGGGAGTTCTTTTAACTGAGATCTACACCAAAGTCAACGAGAAGGTTTATGTGGATACTGTTGGAGATCCTGAAAAGTACAGAATTAAGTTGTCTGAAAAATTTCCCAATGTAAAATTTGTAGTTGCAAAGAAAGCTGATAGTTTATATCCCGTTGTCAGTGGGGCAAGCATAGTCGCAAAGGTCACAAGAGATCGTGCATTGCGGGAATGGGCATTGGAGGAAACAGCTGAAAACATGCATAGAAACTTTGGTTCTGGATACCCTGGAGCATACTATAATCAGGTTTTCATTGCACAGGATCCAACAACTAAATGTTGGCTAGAAGATCACAAGCACTCGGTGTTTGGGTTCCCCACATTAGTCCGCTTCAGTTGGGGTACCTGCAGTTCATATTTCAAAGACGTTGTTGAAGTGGCCCTGGCATTCATAACATTGAAACCTATCTTTCAAAATCCTGTGCCACATGGTTACGCGGAGTCGGATAAAGTGGATGAAGGTGGGGCAACGTCTGGAAGTGGTAAGAAACAATTAAGACTAAGGAATGTTGGTATCACCACATCCAAGAGAAAGAGTGAAGAAATTGAATCAAATGGCAAAGGACGATGCAAGTTTTTCCAGGCTAGAAAACTTGAACAACTCACTCATTTT

Protein sequence

GSYENNQNKSSFHGGNDLKEENTQSKKRSSKESKRDFLKSAMSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEEKREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVLLTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRFSWGTCSSYFKDVVEVALAFITLKPIFQNPVPHGYAESDKVDEGGATSGSGKKQLRLRNVGITTSKRKSEEIESNGKGRCKFFQARKLEQLTHF

Homology

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BLAST of Lsi07G000450 vs. ExPASy Swiss-Prot
Match: Q9SEZ6 (Ribonuclease H2 subunit A OS=Arabidopsis thaliana OX=3702 GN=At2g25100 PE=2 SV=2)

HSP 1 Score: 412.9 bits (1060), Expect = 3.8e-114
Identity = 211/333 (63.36%), Postives = 255/333 (76.58%), Query Frame = 0


Query: 42  MSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEE 101
           M S+   P+WAS+PC+MGIDEAGRGPVLGPMVYGC+YC  SY   L+SL+FADSKTLKEE
Sbjct: 1   MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEE 60

Query: 102 KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 161
           KREEL+E LK ++S+GWAVDVIDPRELS+KML K K NLNEISH+SAMGL+ +VL MGVL
Sbjct: 61  KREELYESLKLDKSLGWAVDVIDPRELSAKMLAKNKTNLNEISHNSAMGLIKRVLDMGVL 120

Query: 162 LTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 221
           LTE         Y+DTVGDP+KYRIKLSE+FP++KFVV+KKADSL+P+VSGASIVAKVTR
Sbjct: 121 LTE--------AYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTR 180

Query: 222 DRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRF 281
           DRAL+EW +EET E+++RNFGSGYPG          DP TK WL  HKHSVFGFP+LVRF
Sbjct: 181 DRALKEWLVEETGEDINRNFGSGYPG----------DPETKAWLVQHKHSVFGFPSLVRF 240

Query: 282 SWGTCSSYFKDVVEVALAFITLKPIFQNPVPHGYAESDKVDEGGATSGSGKKQLRLRNVG 341
           SWGTC+++ K  VEVA                   E+D+ +E G    S K+Q +L + G
Sbjct: 241 SWGTCTTHLKGEVEVAW------------------EADENEESG-NGSSSKRQAKLSSFG 296

Query: 342 ITTSKRKSEEIESNGKGRCKFFQARKLEQLTHF 375
             T +++SEEIES+GKGRCKF QARK++QLT F
Sbjct: 301 FKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF 296

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BLAST of Lsi07G000450 vs. ExPASy Swiss-Prot
Match: O75792 (Ribonuclease H2 subunit A OS=Homo sapiens OX=9606 GN=RNASEH2A PE=1 SV=2)

HSP 1 Score: 234.6 bits (597), Expect = 1.9e-60
Identity = 118/250 (47.20%), Postives = 158/250 (63.20%), Query Frame = 0


Query: 47  PLPKWASK-PCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEEKREE 106
           P+P    K PCV+G+DEAGRGPVLGPMVY   YC    L +L +L  ADSKTL E +RE 
Sbjct: 19  PVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERER 78

Query: 107 LFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVLLTEI 166
           LF  ++  + +GWA+DV+ P  +S+ ML +VK NLN +SHD+A GL+   L  GV +T+ 
Sbjct: 79  LFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQ- 138

Query: 167 YTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRAL 226
                  V+VDTVG PE Y+ +L + FP ++  V  KAD+LYPVVS ASI AKV RD+A+
Sbjct: 139 -------VFVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAV 198

Query: 227 REWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRFSWGT 286
           ++W   E  +++  ++GSGYP           DP TK WL++H   VFGFP  VRFSW T
Sbjct: 199 KKWQFVEKLQDLDTDYGSGYP----------NDPKTKAWLKEHVEPVFGFPQFVRFSWRT 250

Query: 287 CSSYFKDVVE 296
             +  +   E
Sbjct: 259 AQTILEKEAE 250

[Close]

BLAST of Lsi07G000450 vs. ExPASy Swiss-Prot
Match: Q2TBT5 (Ribonuclease H2 subunit A OS=Bos taurus OX=9913 GN=RNASEH2A PE=1 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 3.5e-59
Identity = 120/255 (47.06%), Postives = 160/255 (62.75%), Query Frame = 0


Query: 42  MSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEE 101
           +SS  P P    +PCV+G+DEAGRGPVLGPMVY   YC  S L++L +L  ADSKTL E 
Sbjct: 16  LSSPVP-PVCLKEPCVLGVDEAGRGPVLGPMVYAICYCPLSRLEDLEALKVADSKTLSES 75

Query: 102 KREELFEDLKAN-ESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGV 161
           +R+ LF  ++ + + +GWA+DV+ P  +S+ ML +VK NLN +SHD+A GLV   L  GV
Sbjct: 76  ERDRLFAKMEEDGDFVGWALDVLSPNLISTSMLGRVKYNLNALSHDTATGLVQFALDQGV 135

Query: 162 LLTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVT 221
            + +        V+VDTVG PE Y+ +L ++FP ++  V  KAD+LYPVVS ASI AKV 
Sbjct: 136 NVAQ--------VFVDTVGLPETYQERLQQRFPGIEVTVKAKADALYPVVSAASICAKVA 195

Query: 222 RDRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVR 281
           RD+A++ W   E  +++  ++GSGYP           DP TK WL  H   VFGFP  VR
Sbjct: 196 RDQAVKNWKFVEKLQDLDTDYGSGYP----------NDPKTKAWLRKHVDPVFGFPQFVR 251

Query: 282 FSWGTCSSYFKDVVE 296
           FSW T  S  +   E
Sbjct: 256 FSWRTAQSILESEAE 251

[Close]

BLAST of Lsi07G000450 vs. ExPASy Swiss-Prot
Match: Q5U209 (Ribonuclease H2 subunit A OS=Rattus norvegicus OX=10116 GN=Rnaseh2a PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 3.5e-59
Identity = 116/251 (46.22%), Postives = 158/251 (62.95%), Query Frame = 0


Query: 47  PLPKWASK-PCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEEKREE 106
           P+P    K PCV+G+DEAGRGPVLGPMVY   YC  S L +L +L  ADSKTL E +RE 
Sbjct: 19  PVPAVCLKEPCVLGVDEAGRGPVLGPMVYAICYCPLSRLADLEALKVADSKTLTENERER 78

Query: 107 LFEDLKAN-ESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVLLTE 166
           LF  ++ + + +GWA+D++ P  +S+ ML +VK NLN +SHD+A GL+   +   V +T+
Sbjct: 79  LFAKMEEDGDFVGWALDILSPNLISTSMLGRVKYNLNSMSHDTAAGLIQHAMDQNVKVTQ 138

Query: 167 IYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRA 226
                   V+VDTVG PE Y+ +L ++FP ++  V  KADSL+PVVS ASI+AKV RD+A
Sbjct: 139 --------VFVDTVGMPETYQARLQQRFPGIEVTVKAKADSLFPVVSAASIIAKVARDQA 198

Query: 227 LREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRFSWG 286
           ++ W   E+ + +  ++GSGYP           DP TK WL  H   VFGFP  VRFSW 
Sbjct: 199 VKNWQFVESLQGLDSDYGSGYP----------NDPKTKAWLRKHVDPVFGFPQFVRFSWS 251

Query: 287 TCSSYFKDVVE 296
           T  +  +   E
Sbjct: 259 TAQAILEKEAE 251

[Close]

BLAST of Lsi07G000450 vs. ExPASy Swiss-Prot
Match: Q9CWY8 (Ribonuclease H2 subunit A OS=Mus musculus OX=10090 GN=Rnaseh2a PE=1 SV=2)

HSP 1 Score: 228.8 bits (582), Expect = 1.0e-58
Identity = 118/251 (47.01%), Postives = 156/251 (62.15%), Query Frame = 0


Query: 47  PLPKWASK-PCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEEKREE 106
           P+P    K PCV+G+DEAGRGPVLGPMVY   YC  S L +L +L  ADSKTL E +RE 
Sbjct: 19  PVPAVCLKEPCVLGVDEAGRGPVLGPMVYAICYCPLSRLADLEALKVADSKTLTENERER 78

Query: 107 LFEDLKAN-ESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVLLTE 166
           LF  ++ + + +GWA+DV+ P  +S+ ML +VK NLN +SHD+A GL+   L   V +T+
Sbjct: 79  LFAKMEEDGDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTAAGLIQYALDQNVNVTQ 138

Query: 167 IYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRA 226
                   V+VDTVG PE Y+ +L + FP ++  V  KADSL+PVVS ASI AKV RD+A
Sbjct: 139 --------VFVDTVGMPETYQARLQQHFPGIEVTVKAKADSLFPVVSAASIFAKVARDKA 198

Query: 227 LREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRFSWG 286
           ++ W   E  +++  ++GSGYP           DP TK WL  H   VFGFP  VRFSW 
Sbjct: 199 VKNWQFVENLQDLDSDYGSGYP----------NDPKTKAWLRKHVDPVFGFPQFVRFSWS 251

Query: 287 TCSSYFKDVVE 296
           T  +  +   E
Sbjct: 259 TAQAILEKEAE 251

[Close]

BLAST of Lsi07G000450 vs. ExPASy TrEMBL
Match: A0A6J1IPY0 (Ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111479443 PE=3 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 2.0e-150
Identity = 281/333 (84.38%), Postives = 287/333 (86.19%), Query Frame = 0


Query: 42  MSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEE 101
           MSSQ PLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYLKELSSLNFADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 102 KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 161
           KREELFE LKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 162 LTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 221
           LTE        VYVDTVGDPEKYRIKLSEKFPNVKFVV+KKADSLYPVVSGASIVAKVTR
Sbjct: 121 LTE--------VYVDTVGDPEKYRIKLSEKFPNVKFVVSKKADSLYPVVSGASIVAKVTR 180

Query: 222 DRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRF 281
           DR LR+WALEETAENMHRNFGSGYPG          DPTTKCWLEDHKHSVFGFP+LVRF
Sbjct: 181 DRVLRDWALEETAENMHRNFGSGYPG----------DPTTKCWLEDHKHSVFGFPSLVRF 240

Query: 282 SWGTCSSYFKDVVEVALAFITLKPIFQNPVPHGYAESDKVDEGGATSGSGKKQLRLRNVG 341
           SWGTC+SYFKD+VEV+                   ESDKVDE GAT GSGKKQLRL NVG
Sbjct: 241 SWGTCTSYFKDIVEVSW------------------ESDKVDEDGATGGSGKKQLRLSNVG 297

Query: 342 ITTSKRKSEEIESNGKGRCKFFQARKLEQLTHF 375
           ITTSKRKSEEIESN KGRCKFFQARKLEQLTHF
Sbjct: 301 ITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of Lsi07G000450 vs. ExPASy TrEMBL
Match: A0A6J1GTR5 (Ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111457459 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 3.4e-150
Identity = 281/333 (84.38%), Postives = 285/333 (85.59%), Query Frame = 0


Query: 42  MSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEE 101
           MSSQ PLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYLKELSSLNFADSK LKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKALKEE 60

Query: 102 KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 161
           KREELFE LK NESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 162 LTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 221
           LTE        VYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR
Sbjct: 121 LTE--------VYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 180

Query: 222 DRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRF 281
           DR LR+WALEETAENMHRNFGSGYPG          DPTTKCWLEDHKHSVFGFPTLVRF
Sbjct: 181 DRVLRDWALEETAENMHRNFGSGYPG----------DPTTKCWLEDHKHSVFGFPTLVRF 240

Query: 282 SWGTCSSYFKDVVEVALAFITLKPIFQNPVPHGYAESDKVDEGGATSGSGKKQLRLRNVG 341
           SWGTC+SYFKD+VEV+                   ESDKVDE GAT GSGKKQLRL NVG
Sbjct: 241 SWGTCTSYFKDIVEVSW------------------ESDKVDEDGATGGSGKKQLRLSNVG 297

Query: 342 ITTSKRKSEEIESNGKGRCKFFQARKLEQLTHF 375
           ITTSKRKSEEIESN KGRCKFFQARKLEQLTHF
Sbjct: 301 ITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of Lsi07G000450 vs. ExPASy TrEMBL
Match: A0A0A0KTZ4 (Ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_4G015800 PE=3 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 7.6e-150
Identity = 281/333 (84.38%), Postives = 288/333 (86.49%), Query Frame = 0


Query: 42  MSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEE 101
           MSSQ  LPKW SKPCVMGIDEAGRGPVLGPMVYGCLYCA+SYLK+LSSLNFADSKTLKEE
Sbjct: 1   MSSQDSLPKWTSKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKQLSSLNFADSKTLKEE 60

Query: 102 KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 161
           KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVL MGVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLSMGVL 120

Query: 162 LTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 221
           LTE        VYVDTVGDPEKYRIKLSEKFPN+KFVVAKKADSLYPVVSGASIVAKVTR
Sbjct: 121 LTE--------VYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVAKVTR 180

Query: 222 DRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRF 281
           DRALREWALEETAENMHRNFGSGYPG          DPTTKCWLEDHKHSVFGFPTLVRF
Sbjct: 181 DRALREWALEETAENMHRNFGSGYPG----------DPTTKCWLEDHKHSVFGFPTLVRF 240

Query: 282 SWGTCSSYFKDVVEVALAFITLKPIFQNPVPHGYAESDKVDEGGATSGSGKKQLRLRNVG 341
           SWGTC+SYFKDVVEV+                   ESDKVDE GAT GSGKKQLRL NVG
Sbjct: 241 SWGTCTSYFKDVVEVSW------------------ESDKVDEDGATGGSGKKQLRLSNVG 297

Query: 342 ITTSKRKSEEIESNGKGRCKFFQARKLEQLTHF 375
           ITTSKRKSEEIES+GKGRCKFFQARKLEQL+ F
Sbjct: 301 ITTSKRKSEEIESSGKGRCKFFQARKLEQLSRF 297

[Close]

BLAST of Lsi07G000450 vs. ExPASy TrEMBL
Match: A0A5A7TT92 (Ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001310 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.9e-149
Identity = 281/333 (84.38%), Postives = 286/333 (85.89%), Query Frame = 0


Query: 42  MSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEE 101
           MS Q  LPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCA+SYLKELSSLNFADSKTLKEE
Sbjct: 1   MSLQDSLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKELSSLNFADSKTLKEE 60

Query: 102 KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 161
           KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISH SAMGLVTKVL MGVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHGSAMGLVTKVLSMGVL 120

Query: 162 LTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 221
           LTE        VYVDTVGDPEKYRIKLSEKFPN+KFVVAKKADSLYPVVSGASIVAKVTR
Sbjct: 121 LTE--------VYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVAKVTR 180

Query: 222 DRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRF 281
           DRALREWALEETAENMHRNFGSGYPG          DPTTKCWLEDHKHSVFGFPTLVRF
Sbjct: 181 DRALREWALEETAENMHRNFGSGYPG----------DPTTKCWLEDHKHSVFGFPTLVRF 240

Query: 282 SWGTCSSYFKDVVEVALAFITLKPIFQNPVPHGYAESDKVDEGGATSGSGKKQLRLRNVG 341
           SWGTC+SYFKDVVEV+                   ESDKVDE GAT GSGKKQLRL NVG
Sbjct: 241 SWGTCTSYFKDVVEVSW------------------ESDKVDEDGATGGSGKKQLRLSNVG 297

Query: 342 ITTSKRKSEEIESNGKGRCKFFQARKLEQLTHF 375
           ITTSKRKSEEIESN KGRCKFFQARKLEQL+ F
Sbjct: 301 ITTSKRKSEEIESNAKGRCKFFQARKLEQLSRF 297

[Close]

BLAST of Lsi07G000450 vs. ExPASy TrEMBL
Match: A0A1S3BXP1 (Ribonuclease OS=Cucumis melo OX=3656 GN=LOC103494542 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.9e-149
Identity = 281/333 (84.38%), Postives = 286/333 (85.89%), Query Frame = 0


Query: 42  MSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEE 101
           MS Q  LPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCA+SYLKELSSLNFADSKTLKEE
Sbjct: 1   MSLQDSLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKELSSLNFADSKTLKEE 60

Query: 102 KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 161
           KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISH SAMGLVTKVL MGVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHGSAMGLVTKVLSMGVL 120

Query: 162 LTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 221
           LTE        VYVDTVGDPEKYRIKLSEKFPN+KFVVAKKADSLYPVVSGASIVAKVTR
Sbjct: 121 LTE--------VYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVAKVTR 180

Query: 222 DRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRF 281
           DRALREWALEETAENMHRNFGSGYPG          DPTTKCWLEDHKHSVFGFPTLVRF
Sbjct: 181 DRALREWALEETAENMHRNFGSGYPG----------DPTTKCWLEDHKHSVFGFPTLVRF 240

Query: 282 SWGTCSSYFKDVVEVALAFITLKPIFQNPVPHGYAESDKVDEGGATSGSGKKQLRLRNVG 341
           SWGTC+SYFKDVVEV+                   ESDKVDE GAT GSGKKQLRL NVG
Sbjct: 241 SWGTCTSYFKDVVEVSW------------------ESDKVDEDGATGGSGKKQLRLSNVG 297

Query: 342 ITTSKRKSEEIESNGKGRCKFFQARKLEQLTHF 375
           ITTSKRKSEEIESN KGRCKFFQARKLEQL+ F
Sbjct: 301 ITTSKRKSEEIESNAKGRCKFFQARKLEQLSRF 297

[Close]

BLAST of Lsi07G000450 vs. NCBI nr
Match: XP_038902264.1 (ribonuclease H2 subunit A [Benincasa hispida])

HSP 1 Score: 550.1 bits (1416), Expect = 1.5e-152
Identity = 284/333 (85.29%), Postives = 293/333 (87.99%), Query Frame = 0


Query: 42  MSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEE 101
           MSSQYP+PKWASKPCVMGIDEAGRGPVLGPMVYGCLYCA+SYLKELSSLNFADSKTLKEE
Sbjct: 1   MSSQYPVPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKELSSLNFADSKTLKEE 60

Query: 102 KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 161
           KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLG+GVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGIGVL 120

Query: 162 LTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 221
           LTE        VYVDTVGDPEKYRIKLSEKFP+VKFVVAKKADSLYPVVSGASIVAKVTR
Sbjct: 121 LTE--------VYVDTVGDPEKYRIKLSEKFPSVKFVVAKKADSLYPVVSGASIVAKVTR 180

Query: 222 DRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRF 281
           DRALREWALEETAENMHRNFGSGYPG          DPTTKCWLEDHKHSVFGFPTLVRF
Sbjct: 181 DRALREWALEETAENMHRNFGSGYPG----------DPTTKCWLEDHKHSVFGFPTLVRF 240

Query: 282 SWGTCSSYFKDVVEVALAFITLKPIFQNPVPHGYAESDKVDEGGATSGSGKKQLRLRNVG 341
           SWGTC+SYFKD+VEV+                   ESDKVDE GAT+GSGKKQLRL +VG
Sbjct: 241 SWGTCTSYFKDIVEVSW------------------ESDKVDEDGATTGSGKKQLRLSSVG 297

Query: 342 ITTSKRKSEEIESNGKGRCKFFQARKLEQLTHF 375
           ITTSKRKSEEIESN KGRCKFFQARKLEQLTHF
Sbjct: 301 ITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of Lsi07G000450 vs. NCBI nr
Match: XP_023527419.1 (ribonuclease H2 subunit A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 544.7 bits (1402), Expect = 6.4e-151
Identity = 283/333 (84.98%), Postives = 287/333 (86.19%), Query Frame = 0


Query: 42  MSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEE 101
           MSSQ PLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYLKELSSLNFADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 102 KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 161
           KREELFE LKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 162 LTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 221
           LTE        VYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR
Sbjct: 121 LTE--------VYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 180

Query: 222 DRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRF 281
           DR LR+WALEETAENMHRNFGSGYPG          DPTTKCWLEDHKHSVFGFPTLVRF
Sbjct: 181 DRVLRDWALEETAENMHRNFGSGYPG----------DPTTKCWLEDHKHSVFGFPTLVRF 240

Query: 282 SWGTCSSYFKDVVEVALAFITLKPIFQNPVPHGYAESDKVDEGGATSGSGKKQLRLRNVG 341
           SWGTC+SYFKD+VEV+                   ESDKVDE GAT GSGKKQLRL NVG
Sbjct: 241 SWGTCTSYFKDIVEVSW------------------ESDKVDEDGATGGSGKKQLRLSNVG 297

Query: 342 ITTSKRKSEEIESNGKGRCKFFQARKLEQLTHF 375
           ITTSKRKSEEIESN KGRCKFFQARKLEQLTHF
Sbjct: 301 ITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of Lsi07G000450 vs. NCBI nr
Match: XP_022979887.1 (ribonuclease H2 subunit A [Cucurbita maxima])

HSP 1 Score: 542.0 bits (1395), Expect = 4.1e-150
Identity = 281/333 (84.38%), Postives = 287/333 (86.19%), Query Frame = 0


Query: 42  MSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEE 101
           MSSQ PLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYLKELSSLNFADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 102 KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 161
           KREELFE LKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 162 LTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 221
           LTE        VYVDTVGDPEKYRIKLSEKFPNVKFVV+KKADSLYPVVSGASIVAKVTR
Sbjct: 121 LTE--------VYVDTVGDPEKYRIKLSEKFPNVKFVVSKKADSLYPVVSGASIVAKVTR 180

Query: 222 DRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRF 281
           DR LR+WALEETAENMHRNFGSGYPG          DPTTKCWLEDHKHSVFGFP+LVRF
Sbjct: 181 DRVLRDWALEETAENMHRNFGSGYPG----------DPTTKCWLEDHKHSVFGFPSLVRF 240

Query: 282 SWGTCSSYFKDVVEVALAFITLKPIFQNPVPHGYAESDKVDEGGATSGSGKKQLRLRNVG 341
           SWGTC+SYFKD+VEV+                   ESDKVDE GAT GSGKKQLRL NVG
Sbjct: 241 SWGTCTSYFKDIVEVSW------------------ESDKVDEDGATGGSGKKQLRLSNVG 297

Query: 342 ITTSKRKSEEIESNGKGRCKFFQARKLEQLTHF 375
           ITTSKRKSEEIESN KGRCKFFQARKLEQLTHF
Sbjct: 301 ITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of Lsi07G000450 vs. NCBI nr
Match: XP_022955431.1 (ribonuclease H2 subunit A [Cucurbita moschata])

HSP 1 Score: 541.2 bits (1393), Expect = 7.1e-150
Identity = 281/333 (84.38%), Postives = 285/333 (85.59%), Query Frame = 0


Query: 42  MSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEE 101
           MSSQ PLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYLKELSSLNFADSK LKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKALKEE 60

Query: 102 KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 161
           KREELFE LK NESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 162 LTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 221
           LTE        VYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR
Sbjct: 121 LTE--------VYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 180

Query: 222 DRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRF 281
           DR LR+WALEETAENMHRNFGSGYPG          DPTTKCWLEDHKHSVFGFPTLVRF
Sbjct: 181 DRVLRDWALEETAENMHRNFGSGYPG----------DPTTKCWLEDHKHSVFGFPTLVRF 240

Query: 282 SWGTCSSYFKDVVEVALAFITLKPIFQNPVPHGYAESDKVDEGGATSGSGKKQLRLRNVG 341
           SWGTC+SYFKD+VEV+                   ESDKVDE GAT GSGKKQLRL NVG
Sbjct: 241 SWGTCTSYFKDIVEVSW------------------ESDKVDEDGATGGSGKKQLRLSNVG 297

Query: 342 ITTSKRKSEEIESNGKGRCKFFQARKLEQLTHF 375
           ITTSKRKSEEIESN KGRCKFFQARKLEQLTHF
Sbjct: 301 ITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of Lsi07G000450 vs. NCBI nr
Match: KAG6582048.1 (Ribonuclease H2 subunit A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 540.0 bits (1390), Expect = 1.6e-149
Identity = 281/333 (84.38%), Postives = 285/333 (85.59%), Query Frame = 0


Query: 42  MSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEE 101
           MSSQ PLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYLKELSSLNFADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 102 KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 161
           KREELFE LK NESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 162 LTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 221
           LTE        VYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR
Sbjct: 121 LTE--------VYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 180

Query: 222 DRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRF 281
           DR LR+WALEETAENMHRNFGSGYPG          DPTTKCWLEDHKHSVFGFPTLVRF
Sbjct: 181 DRVLRDWALEETAENMHRNFGSGYPG----------DPTTKCWLEDHKHSVFGFPTLVRF 240

Query: 282 SWGTCSSYFKDVVEVALAFITLKPIFQNPVPHGYAESDKVDEGGATSGSGKKQLRLRNVG 341
           SWGTC+SYFKD+VEV+                   ESDKVDE GAT GSGKKQLRL NVG
Sbjct: 241 SWGTCTSYFKDIVEVSW------------------ESDKVDEDGATGGSGKKQLRLSNVG 297

Query: 342 ITTSKRKSEEIESNGKGRCKFFQARKLEQLTHF 375
           ITTSKRKSEEIESN KGRCKFFQARKLEQLT F
Sbjct: 301 ITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 297

[Close]

BLAST of Lsi07G000450 vs. TAIR 10
Match: AT2G25100.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 412.9 bits (1060), Expect = 2.7e-115
Identity = 211/333 (63.36%), Postives = 255/333 (76.58%), Query Frame = 0


Query: 42  MSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEE 101
           M S+   P+WAS+PC+MGIDEAGRGPVLGPMVYGC+YC  SY   L+SL+FADSKTLKEE
Sbjct: 1   MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEE 60

Query: 102 KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 161
           KREEL+E LK ++S+GWAVDVIDPRELS+KML K K NLNEISH+SAMGL+ +VL MGVL
Sbjct: 61  KREELYESLKLDKSLGWAVDVIDPRELSAKMLAKNKTNLNEISHNSAMGLIKRVLDMGVL 120

Query: 162 LTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 221
           LTE         Y+DTVGDP+KYRIKLSE+FP++KFVV+KKADSL+P+VSGASIVAKVTR
Sbjct: 121 LTE--------AYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTR 180

Query: 222 DRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRF 281
           DRAL+EW +EET E+++RNFGSGYPG          DP TK WL  HKHSVFGFP+LVRF
Sbjct: 181 DRALKEWLVEETGEDINRNFGSGYPG----------DPETKAWLVQHKHSVFGFPSLVRF 240

Query: 282 SWGTCSSYFKDVVEVALAFITLKPIFQNPVPHGYAESDKVDEGGATSGSGKKQLRLRNVG 341
           SWGTC+++ K  VEVA                   E+D+ +E G    S K+Q +L + G
Sbjct: 241 SWGTCTTHLKGEVEVAW------------------EADENEESG-NGSSSKRQAKLSSFG 296

Query: 342 ITTSKRKSEEIESNGKGRCKFFQARKLEQLTHF 375
             T +++SEEIES+GKGRCKF QARK++QLT F
Sbjct: 301 FKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF 296

[Close]

BLAST of Lsi07G000450 vs. TAIR 10
Match: AT2G25100.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 377.1 bits (967), Expect = 1.6e-104
Identity = 198/333 (59.46%), Postives = 239/333 (71.77%), Query Frame = 0


Query: 42  MSSQYPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCATSYLKELSSLNFADSKTLKEE 101
           M S+   P+WAS+PC+MGIDEAGRGPVLGPMVYGC+YC  SY   L+SL+FADSKTLKEE
Sbjct: 1   MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEE 60

Query: 102 KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 161
           KREEL+E LK ++S+GWAVDVIDPRELS+KML                    KVL MGVL
Sbjct: 61  KREELYESLKLDKSLGWAVDVIDPRELSAKML-------------------AKVLDMGVL 120

Query: 162 LTEIYTKVNEKVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTR 221
           LTE         Y+DTVGDP+KYRIKLSE+FP++KFVV+KKADSL+P+VSGASIVAKVTR
Sbjct: 121 LTE--------AYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTR 180

Query: 222 DRALREWALEETAENMHRNFGSGYPGAYYNQVFIAQDPTTKCWLEDHKHSVFGFPTLVRF 281
           DRAL+EW +EET E+++RNFGSGYPG          DP TK WL  HKHSVFGFP+LVRF
Sbjct: 181 DRALKEWLVEETGEDINRNFGSGYPG----------DPETKAWLVQHKHSVFGFPSLVRF 240

Query: 282 SWGTCSSYFKDVVEVALAFITLKPIFQNPVPHGYAESDKVDEGGATSGSGKKQLRLRNVG 341
           SWGTC+++ K  VEVA                   E+D+ +E G    S K+Q +L + G
Sbjct: 241 SWGTCTTHLKGEVEVAW------------------EADENEESG-NGSSSKRQAKLSSFG 277

Query: 342 ITTSKRKSEEIESNGKGRCKFFQARKLEQLTHF 375
             T +++SEEIES+GKGRCKF QARK++QLT F
Sbjct: 301 FKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF 277

The following BLAST results are available for this feature:

BLAST of Lsi07G000450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SEZ6	3.8e-114	63.36	Ribonuclease H2 subunit A OS=Arabidopsis thaliana OX=3702 GN=At2g25100 PE=2 SV=2	[more]
O75792	1.9e-60	47.20	Ribonuclease H2 subunit A OS=Homo sapiens OX=9606 GN=RNASEH2A PE=1 SV=2	[more]
Q2TBT5	3.5e-59	47.06	Ribonuclease H2 subunit A OS=Bos taurus OX=9913 GN=RNASEH2A PE=1 SV=1	[more]
Q5U209	3.5e-59	46.22	Ribonuclease H2 subunit A OS=Rattus norvegicus OX=10116 GN=Rnaseh2a PE=2 SV=1	[more]
Q9CWY8	1.0e-58	47.01	Ribonuclease H2 subunit A OS=Mus musculus OX=10090 GN=Rnaseh2a PE=1 SV=2	[more]

BLAST of Lsi07G000450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IPY0	2.0e-150	84.38	Ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111479443 PE=3 SV=1	[more]
A0A6J1GTR5	3.4e-150	84.38	Ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111457459 PE=3 SV=1	[more]
A0A0A0KTZ4	7.6e-150	84.38	Ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_4G015800 PE=3 SV=1	[more]
A0A5A7TT92	4.9e-149	84.38	Ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001310 ...	[more]
A0A1S3BXP1	4.9e-149	84.38	Ribonuclease OS=Cucumis melo OX=3656 GN=LOC103494542 PE=3 SV=1	[more]

BLAST of Lsi07G000450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902264.1	1.5e-152	85.29	ribonuclease H2 subunit A [Benincasa hispida]	[more]
XP_023527419.1	6.4e-151	84.98	ribonuclease H2 subunit A [Cucurbita pepo subsp. pepo]	[more]
XP_022979887.1	4.1e-150	84.38	ribonuclease H2 subunit A [Cucurbita maxima]	[more]
XP_022955431.1	7.1e-150	84.38	ribonuclease H2 subunit A [Cucurbita moschata]	[more]
KAG6582048.1	1.6e-149	84.38	Ribonuclease H2 subunit A, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lsi07G000450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G25100.1	2.7e-115	63.36	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G25100.2	1.6e-104	59.46	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 25..235 e-value: 1.4E-65 score: 222.7
IPR024567	Ribonuclease HII/HIII domain	PFAM	PF01351	RNase_HII	coord: 58..285 e-value: 4.3E-44 score: 150.7
IPR023160	Ribonuclease HII, helix-loop-helix cap domain superfamily	GENE3D	1.10.10.460	Ribonuclease hii. Domain 2	coord: 236..293 e-value: 1.4E-14 score: 55.5
IPR004649	Ribonuclease H2, subunit A	TIGRFAM	TIGR00729	TIGR00729	coord: 57..288 e-value: 5.9E-49 score: 164.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..36
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 14..36
None	No IPR available	PANTHER	PTHR10954:SF19	RIBONUCLEASE	coord: 298..373 coord: 47..297
None	No IPR available	CDD	cd07181	RNase_HII_eukaryota_like	coord: 58..292 e-value: 1.59646E-134 score: 381.096
IPR001352	Ribonuclease HII/HIII	PANTHER	PTHR10954	RIBONUCLEASE H2 SUBUNIT A	coord: 298..373 coord: 47..297
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 56..291

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi07G000450.1	Lsi07G000450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043137	DNA replication, removal of RNA primer
biological_process	GO:0006298	mismatch repair
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0016070	RNA metabolic process
cellular_component	GO:0032299	ribonuclease H2 complex
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity
molecular_function	GO:0003676	nucleic acid binding