Lsi07G000360 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi07G000360 vs. ExPASy Swiss-Prot
Match: F4JLM5 (Isoleucine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g10320 PE=2 SV=1)

HSP 1 Score: 1852.8 bits (4798), Expect = 0.0e+00
Identity = 905/1196 (75.67%), Postives = 1026/1196 (85.79%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            M+EVCEGK+FSFP+ EE VL +W++I AF+TQL+RT +LPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MEEVCEGKEFSFPRQEEDVLSFWTEIDAFKTQLKRTENLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQ MTGHHVTRRFGWDCHGLPVENEID+KL IKRRD+V+KMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQTMTGHHVTRRFGWDCHGLPVENEIDRKLNIKRRDEVIKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYV EWEK+ITR GRWIDFKNDYKTMDL FMESVWWVFSQL+EK LVY+GFKVM
Sbjct: 121  CRSIVTRYVAEWEKVITRCGRWIDFKNDYKTMDLPFMESVWWVFSQLWEKNLVYRGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQ+YK+VPDPEIMV FPV+GD  NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQNYKEVPDPEIMVTFPVIGDQDNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPN--ANDAINKSNPKKKGSSNSK 300
            NA FVYVKVRNK +GKVY+VA+SRLSALP +K K    N  A D   K+NPK KG   +K
Sbjct: 241  NAKFVYVKVRNKNTGKVYIVAESRLSALPTDKPKAKLSNGPAGDT-KKANPKAKG---AK 300

Query: 301  TENAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCA 360
             E+A  DS+E+LE+  GASLVGKKY PLFDYF++FS  AFRVVAD YVTDDSGTGIVHCA
Sbjct: 301  PESAA-DSYEVLEKFNGASLVGKKYEPLFDYFSDFSSEAFRVVADDYVTDDSGTGIVHCA 360

Query: 361  PAFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQG 420
            PAFGEDDYRVC+ N++I KGENL+VAVDDDG FT +IT FSGRYVKDADKDIIEAVKA+G
Sbjct: 361  PAFGEDDYRVCLLNKIIKKGENLVVAVDDDGLFTERITHFSGRYVKDADKDIIEAVKAKG 420

Query: 421  RLVKSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCD---------KEKRF 480
            RLVK+GSFTHSYPFCWRSDTPLIYRAVPSW V +  + + ++   +         K+KRF
Sbjct: 421  RLVKTGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEQLKEKLLKSNEQTEWVPGYVKDKRF 480

Query: 481  HNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDH 540
            HNWLENARDWA+SRSRFWGTPLP+W+S+DGEE+++MDS+EKLE+LSGVKVFDLHRH IDH
Sbjct: 481  HNWLENARDWAISRSRFWGTPLPIWISDDGEEVVIMDSVEKLEKLSGVKVFDLHRHHIDH 540

Query: 541  ITIPSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQ 600
            ITIPS RG E+GVLRRV+DVFDCWFESGSMPYAYIHYPFEN ELFEKNFPG FVAEGLDQ
Sbjct: 541  ITIPSSRGDEFGVLRRVEDVFDCWFESGSMPYAYIHYPFENKELFEKNFPGDFVAEGLDQ 600

Query: 601  TRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADAL 660
            TRGWFYTLMVLSTALF KPAF+NLICNGLVLAEDGKKM+K+L+NYP P+E+I++YGADA+
Sbjct: 601  TRGWFYTLMVLSTALFEKPAFKNLICNGLVLAEDGKKMAKKLRNYPPPLEVIDEYGADAV 660

Query: 661  RLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQ 720
            RLYLINSPVVRAE LRFKKEGV GVV+DVFLPWYNAYRFLVQNAKRLE EG  PF P D 
Sbjct: 661  RLYLINSPVVRAEPLRFKKEGVLGVVKDVFLPWYNAYRFLVQNAKRLETEGGVPFVPTDL 720

Query: 721  ATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRL 780
            AT+Q S N+LDQWI+SATQSLV FVR+EMD YRLYTVVP LLKFLDNLTNIYVRFNRKRL
Sbjct: 721  ATIQ-SANILDQWIHSATQSLVRFVREEMDAYRLYTVVPRLLKFLDNLTNIYVRFNRKRL 780

Query: 781  KGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQ 840
            KGRTGE+DC  ALSTL++VLLTSCKVMAPFTPFFTE LYQNLRK   GSEES+HYCS P 
Sbjct: 781  KGRTGEDDCHTALSTLFNVLLTSCKVMAPFTPFFTETLYQNLRKACKGSEESVHYCSIPP 840

Query: 841  EGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLRE 900
              G  GERIE SVTRMM IIDLARNIRER+  PLK PL+EMIVVHPD DFL+DI G LRE
Sbjct: 841  REGMEGERIELSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADFLNDITGVLRE 900

Query: 901  YVLEELNIRSLIPCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEK 960
            YVLEELN+RSL+PC+DTLKYASL+AEPDFSVLGKRLGK+MG+VAKEVK MSQ+DILAFE+
Sbjct: 901  YVLEELNVRSLVPCNDTLKYASLKAEPDFSVLGKRLGKSMGLVAKEVKEMSQKDILAFEE 960

Query: 961  VGEVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAR 1020
             GEVTIA H LK ++IK+VR F+RPD + + EID+AGDGDVLVILDLR D+SL EAG AR
Sbjct: 961  AGEVTIANHLLKETDIKIVRVFKRPDDLKENEIDSAGDGDVLVILDLRADDSLVEAGFAR 1020

Query: 1021 EIVNRIQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPLLPSTVL 1080
            EIVNRIQKLRKK+ LEPTD VEVYF+SLDED SV+++VL +QE  I ++IGS LL S+++
Sbjct: 1021 EIVNRIQKLRKKSGLEPTDFVEVYFQSLDEDESVSKQVLVSQEQNIKDSIGSTLLLSSLM 1080

Query: 1081 PSYAVTLAEESF----------HNVAGISFSIILARPVPMFNSAAITALYEGNEKFARAL 1140
            PS+AV +A+E+F            V  +S+ I LARP   FN  A+ ALY G+ K A  L
Sbjct: 1081 PSHAVIIADETFTPKETSDESVKKVPKLSYKISLARPALKFNEEAVLALYSGDVKSATGL 1140

Query: 1141 EVYLLSRDLSNLKSEFSSGNGKIRVDFIEGQPIVEVLLGDHVFLTAGDQFLKANSS 1176
            + YLLSRD SNLKSEF +G+GKI V  IE  P+  V+LG+H+ L+ GD  L   ++
Sbjct: 1141 QTYLLSRDHSNLKSEFQAGDGKITVSCIENVPVATVVLGEHLHLSVGDDLLSKRNA 1190

BLAST of Lsi07G000360 vs. ExPASy Swiss-Prot
Match: P41252 (Isoleucine--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=IARS1 PE=1 SV=2)

HSP 1 Score: 1163.3 bits (3008), Expect = 0.0e+00
Identity = 579/1106 (52.35%), Postives = 765/1106 (69.17%), Query Frame = 0

Query: 8    KDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKD 67
            ++ +FP  EE +LE+W++   F+  L++++  P++ FYDGPPFATGLPHYGHILAGTIKD
Sbjct: 7    ENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKD 66

Query: 68   IVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTR 127
            IVTRY   +G HV RRFGWDCHGLPVE EID+ LGI+  +DV KMGI +YN +CR+IV R
Sbjct: 67   IVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMR 126

Query: 128  YVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCK 187
            Y  EW+  ++R GRWIDF NDYKT+  +FMESVWWVF QLY+KGLVY+G KVMP+ST C 
Sbjct: 127  YSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACN 186

Query: 188  TPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCVNANFVYV 247
            TPLSNFE+ Q+YKDV DP + V FP L + +  + VAWTTTPWTLPSNLA+CVN    YV
Sbjct: 187  TPLSNFESHQNYKDVQDPSVFVTFP-LEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYV 246

Query: 248  KVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTENAVEDSF 307
            K+++   G++ ++ ++RLSAL                                  +E  +
Sbjct: 247  KIKDVARGRLLILMEARLSAL--------------------------------YKLESDY 306

Query: 308  EILEEVLGASLVGKKYVPLFDYFNEFSD-VAFRVVADSYVTDDSGTGIVHCAPAFGEDDY 367
            EILE   GA L GKKY PLFDYF +  +  AF V+ D+YV ++ GTG+VH AP FG +DY
Sbjct: 307  EILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDY 366

Query: 368  RVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSF 427
            RVC++  +I K    +  VD  GCFTT++TDF+G+YVKDADK II  +K QGRL+ + +F
Sbjct: 367  RVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTF 426

Query: 428  THSYPFCWRSDTPLIYRAVPSWQVHLFSILKSV-----ICF----CDKEKRFHNWLENAR 487
            THSYPFCWRSDTPLIY+AVPSW V + +++  +     +C+      +EKRF NWL++AR
Sbjct: 427  THSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLKDAR 486

Query: 488  DWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHITIPSRRG 547
            DW +SR+R+WGTP+P+WVS+D EE++ + S+ +LE+LSG K+ DLHR  +DH+TIPSR G
Sbjct: 487  DWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTIPSRCG 546

Query: 548  LEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFYTL 607
               G L R+ +VFDCWFESGSMPYA +HYPFEN   FE  FP  F+AEG+DQTRGWFYTL
Sbjct: 547  --KGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTL 606

Query: 608  MVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLINSP 667
            +VL+TALFG+P F+N+I NGLVLA DG+KMSKR KNYP P+ II  YGADALRLYLINSP
Sbjct: 607  LVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSP 666

Query: 668  VVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKSFN 727
            VVRAE LRFK+EGV  V++DV LPWYNAYRFL+QN  RL+ E    F   ++ T+++S N
Sbjct: 667  VVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFL-YNENTVRESPN 726

Query: 728  VLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEED 787
            + D+WI S  QSL+ F   EM  YRLYTVVP L+KF+D LTN YVR NR+RLKG  G ED
Sbjct: 727  ITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMED 786

Query: 788  CRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLR------KVSDGSEESIHYCSYPQEG 847
            C +AL TL+ VLL+ C++MAP+TPF TE++YQNL+       V D    SIHY   P+  
Sbjct: 787  CVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVR 846

Query: 848  GKR-GERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREY 907
             +   ++ E +V++M ++I+L R IR+R   P+K PL+E++V+H D + L DI   L +Y
Sbjct: 847  EELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIK-SLEKY 906

Query: 908  VLEELNIRSLIPCDDTLKYA-SLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEK 967
            ++EELN+R +    D  KY   LRAEPD  VLGKRL  A   V   +K +S E++  F+K
Sbjct: 907  IIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSSEELEQFQK 966

Query: 968  VGEVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAR 1027
             G + +  H L   +I+++  F +  G T  + +A  D   LV+LD+ PD+S+ + G+AR
Sbjct: 967  TGTIVVEGHELHDEDIRLMYTFDQATGGT-AQFEAHSDAQALVLLDVTPDQSMVDEGMAR 1026

Query: 1028 EIVNRIQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPLLPSTVL 1087
            E++NRIQKLRKK  L PTD + VY+K+  E T +   V+ +   +I   I +PL P  V 
Sbjct: 1027 EVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYL-NSVIESHTEFIFTTIKAPLKPYPVS 1071

Query: 1088 PSYAVTLAEESFHNVAGISFSIILAR 1096
            PS  V + E++   + G    I L R
Sbjct: 1087 PSDKVLIQEKT--QLKGSELEITLTR 1071

BLAST of Lsi07G000360 vs. ExPASy Swiss-Prot
Match: Q8BU30 (Isoleucine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Iars1 PE=1 SV=2)

HSP 1 Score: 1158.7 bits (2996), Expect = 0.0e+00
Identity = 580/1091 (53.16%), Postives = 758/1091 (69.48%), Query Frame = 0

Query: 8    KDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKD 67
            ++ SFP  EE +LE+WS    F+  L++++  P++ FYDGPPFATGLPHYGHILAGTIKD
Sbjct: 7    ENISFPAEEEKILEFWSKHNCFQECLKQSKLRPKFTFYDGPPFATGLPHYGHILAGTIKD 66

Query: 68   IVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTR 127
            IVTRY   +G HV RRFGWDCHGLPVE EID+ LGIK  +DV KMGI +YN++CR+IV R
Sbjct: 67   IVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNKQCRAIVMR 126

Query: 128  YVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCK 187
            Y  EW+  +TR GRWIDF NDYKT+  +FMESVWWVF QLY+KGLVY+G KVMP+ST C 
Sbjct: 127  YSAEWKSTVTRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACG 186

Query: 188  TPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCVNANFVYV 247
            TPLSNFE+ Q+YKDV DP + V FP L + +N + VAWTTTPWTLPSNLALCVN    YV
Sbjct: 187  TPLSNFESNQNYKDVQDPSVFVTFP-LEEDENTSLVAWTTTPWTLPSNLALCVNPEIQYV 246

Query: 248  KVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTENAVEDSF 307
            K+++   GK++++ ++RLSAL   KQ                              E  +
Sbjct: 247  KIKDVARGKLFILTEARLSAL--YKQ------------------------------ESDY 306

Query: 308  EILEEVLGASLVGKKYVPLFDYFNEFSD-VAFRVVADSYVTDDSGTGIVHCAPAFGEDDY 367
            EILE   GASL GKKY PLFDYF +  +  AF V+ D YV D+ GTG+VH AP FG DD+
Sbjct: 307  EILERFPGASLKGKKYKPLFDYFIKCKENGAFTVLVDHYVKDEEGTGVVHQAPYFGADDH 366

Query: 368  RVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSF 427
            RVC++  +I K    +  VD  GCFT ++T F G+YVKDADK+II  +K QGRL+ +G+F
Sbjct: 367  RVCMDFNIIQKDSVPVCPVDASGCFTEEVTHFVGQYVKDADKNIIRMLKEQGRLLAAGTF 426

Query: 428  THSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCD---------KEKRFHNWLENAR 487
            THSYPFCWRSDTPLIY++VPSW V +  ++  ++   D         +EKRF NWL+ AR
Sbjct: 427  THSYPFCWRSDTPLIYKSVPSWFVRVEPMVDQLLKNNDLCYWVPEFVREKRFGNWLKEAR 486

Query: 488  DWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHITIPSRRG 547
            DWA+SR+R+WGTP+P+WVSED EE++ + S+ +LE+LSG K+ DLHR  IDH+TIPSR G
Sbjct: 487  DWAISRNRYWGTPIPLWVSEDLEEVVCIGSVAELEELSGTKISDLHRESIDHLTIPSRCG 546

Query: 548  LEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFYTL 607
                 LRRV +VFDCWFESGSMPYA +HYPFE+   FE  FP  F+AEG+DQTRGWFYTL
Sbjct: 547  --KAPLRRVSEVFDCWFESGSMPYAQVHYPFESKREFEDAFPADFIAEGIDQTRGWFYTL 606

Query: 608  MVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLINSP 667
            +VL+TALFG+P F+N+I NGL+LA DG+KMSKR KNYP P+ II+ YGADALRLYLINSP
Sbjct: 607  LVLATALFGQPPFKNVIVNGLILASDGQKMSKRKKNYPDPVSIIDKYGADALRLYLINSP 666

Query: 668  VVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKSFN 727
            VVRAE LRFK+EGV  V++DV LPWYNAYRF +QN  RL  E    F   ++ T+++S N
Sbjct: 667  VVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVFRLHKEEEVKFL-YNEHTVRESPN 726

Query: 728  VLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEED 787
            + D+W+ S  QSL+ F   EM  YRLYTVVP L+KF+D LTN YVR NR+RLKG +G ED
Sbjct: 727  ITDRWVLSFMQSLLGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGESGVED 786

Query: 788  CRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLR------KVSDGSEESIHYCSYPQEG 847
            C +AL TL+ VLL+ C++MAP+TPF TE++YQNL+       + D    SIHY   P+  
Sbjct: 787  CVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKLLIDPASLRDKDTLSIHYLMLPRVR 846

Query: 848  GKR-GERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREY 907
             +   ++ E +V+RM ++I+L R IR+R   P+K PL+E++V+H D + L+DI   L +Y
Sbjct: 847  EELIDKKTENAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALEDIR-SLEKY 906

Query: 908  VLEELNIRSLIPCDDTLKYA-SLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEK 967
            ++EELN+R +    D  KY   LRAEPD  VLGKRL  A   V   +K +S E++  F+K
Sbjct: 907  IIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMMAIKRLSNEELERFQK 966

Query: 968  VGEVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAR 1027
             G + +  H L   +I+++  F +  G T  + +A  D   LV+LD+ PD+S+ + G+AR
Sbjct: 967  SGSIVVEGHELHEEDIRLMYTFDQATGGT-AQFEAHSDAQALVLLDVTPDQSMVDEGMAR 1026

Query: 1028 EIVNRIQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPLLPSTVL 1081
            E++NRIQKLRKK  L PTD + VY+ +  E   +   V+ +   +I   I +PL P  V 
Sbjct: 1027 EVINRIQKLRKKCNLVPTDEITVYYNAKSEGRYL-NNVIESHTDFIFATIKAPLKPYPVP 1058

BLAST of Lsi07G000360 vs. ExPASy Swiss-Prot
Match: Q54YD4 (Probable isoleucine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=ileS PE=3 SV=1)

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 559/1077 (51.90%), Postives = 743/1077 (68.99%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            M+EV +  DF+    E  +L+YW DIKAFET ++ +   PEY FYDGPPFATGLPHYGHI
Sbjct: 1    MEEVPQKIDFA--GEESKILKYWDDIKAFETSVKMSEGKPEYSFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKD +TRY   TGHHV RRFGWDCHGLP+E EID+  G++ ++DVLKMGI  YN+ 
Sbjct: 61   LAGTIKDTITRYAHQTGHHVERRFGWDCHGLPIEFEIDKLHGVRTKEDVLKMGIPTYNQH 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIV +Y  EWE ++ R GRWID KN+YKTMD  FMESVWWVF +L++K LVY+GFKVM
Sbjct: 121  CRSIVMKYSHEWEIVVNRMGRWIDMKNNYKTMDTPFMESVWWVFQELFKKDLVYQGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYS GC TPLSNFEA  +YKDV DP  +V+F  L D +  + +AWTTTPWTLPSNLAL V
Sbjct: 181  PYSIGCTTPLSNFEASSNYKDVSDPACVVSFQTL-DDEKVSILAWTTTPWTLPSNLALTV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            N    Y+K+ +    +++++ K+R S L                 KS             
Sbjct: 241  NPKMEYIKINDIKRNQIFILGKNRTSIL----------------YKS------------- 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDV-AFRVVADSYVTDDSGTGIVHCAP 360
               +  + +LE + G  L+GKKYVP+F YF   +++  F V+   +VTDDSGTGIVH AP
Sbjct: 301  ---DKEYTVLETMKGTDLIGKKYVPMFPYFASDANMGGFVVIGGDFVTDDSGTGIVHTAP 360

Query: 361  AFGEDDYRVCIENQVINKGE---NLIVAVDDDGCFTTKITDFSGRYVKDAD--KDIIEAV 420
            A+GEDD+ VCI N VI++ +    ++ +VD +GCFT+ +TDF+G  VKDA+  K I   +
Sbjct: 361  AYGEDDFNVCIANGVISRDQFKRPILNSVDANGCFTSDVTDFAGMMVKDAETTKQISIYL 420

Query: 421  KAQGRLVKSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCD---------K 480
            K +GR+V S +  HSYP+CWRSDTPLIY+AV SW V + SI   ++   D         K
Sbjct: 421  KNKGRMVNSANLVHSYPYCWRSDTPLIYKAVGSWFVRVESIRDKLLANNDKTYWVPDFVK 480

Query: 481  EKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRH 540
            EKRF NWL+NA DWAVSR+R+WGTP+P+W+SEDGEE++V+ SI++LE+LSGV+V DLHR 
Sbjct: 481  EKRFANWLKNATDWAVSRNRYWGTPIPLWISEDGEEVVVIGSIDELERLSGVRVTDLHRE 540

Query: 541  KIDHITIPSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAE 600
             IDHITIPS++G   G LRR++DVFDCWFESGSMPYA  HYPFEN + FEK FP HF+AE
Sbjct: 541  SIDHITIPSQKG--KGTLRRIEDVFDCWFESGSMPYAQQHYPFENKDKFEKIFPAHFIAE 600

Query: 601  GLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYG 660
            GLDQTRGWFYTL+VLSTALF KP F+NLI NGLVLA DGKKMSKRLKNYP PME+++  G
Sbjct: 601  GLDQTRGWFYTLLVLSTALFDKPPFQNLIVNGLVLAADGKKMSKRLKNYPDPMEVVSKVG 660

Query: 661  ADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFS 720
            AD+LRLYLINSPVVRAETL+F+++GV  +++DVFLPW+NAYRF VQN  R E      F 
Sbjct: 661  ADSLRLYLINSPVVRAETLKFQEKGVQDMIKDVFLPWFNAYRFFVQNCLRFEKATNTTFQ 720

Query: 721  PVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFN 780
            P D      S NV+D+WI ++ QSL+ FVR EM  YRLYTVVP L++F+++LTN +VR N
Sbjct: 721  P-DIKVALASENVMDKWILASCQSLIAFVRAEMAAYRLYTVVPKLVRFIEDLTNWFVRLN 780

Query: 781  RKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKV--SDGSEESIH 840
            RKRLKG  G+ DC  AL+ LY VL+T C  M PFTPFFTE +YQNL+K    +   +S+H
Sbjct: 781  RKRLKGSNGDADCLAALNILYEVLMTICIAMGPFTPFFTEYMYQNLKKALPKEKQMDSVH 840

Query: 841  YCSYPQEGGKR-GERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDD 900
            Y  +P+   +    RIEE+++RM  +I+L R  R+R  KP+K PL+E +V+  ++ +L D
Sbjct: 841  YVMFPEPIQEAFNTRIEEAISRMQVVIELGRAARDRRTKPIKFPLKEFMVITENQQYLAD 900

Query: 901  IAGKLREYVLEELNIRSLIPCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQE 960
            +   L+ Y+LEELNI++++   D   +  + AE D   LG RL      ++  +  ++ E
Sbjct: 901  LE-SLKSYILEELNIQNIVLTSDEGSFVVVTAEADNKRLGARLKNDFKKISPLISKLTNE 960

Query: 961  DILAFEKVGEVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESL 1020
             +  F+K   + I  H L   ++K++R +   +G T    + +G+ ++L +LDL  D +L
Sbjct: 961  QLREFQKTQTIDILGHELTSEDLKIIRKY---NGETTNS-EPSGNEEILTVLDLTVDSAL 1020

Query: 1021 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAI 1060
            +E G+ARE++NR+Q+LRKK+ L   D V +++ + + +   A   +     YI E I
Sbjct: 1021 YEKGLARELINRVQRLRKKSGLTFDDPVSMFYHTKEAELKTA---IENNNDYIKETI 1031

BLAST of Lsi07G000360 vs. ExPASy Swiss-Prot
Match: Q21926 (Isoleucine--tRNA ligase, cytoplasmic OS=Caenorhabditis elegans OX=6239 GN=iars-1 PE=3 SV=1)

HSP 1 Score: 1075.1 bits (2779), Expect = 0.0e+00
Identity = 550/1069 (51.45%), Postives = 707/1069 (66.14%), Query Frame = 0

Query: 9    DFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDI 68
            + +F + E+ V + W D   F+  +E ++D P + FYDGPPFATGLPHYGH+L  TIKD+
Sbjct: 10   NINFAREEDKVAQKWQDENTFQRSVELSKDRPHFTFYDGPPFATGLPHYGHMLTSTIKDV 69

Query: 69   VTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTRY 128
            V R+    GH+V RRFGWD HGLPVE EID+ LGI    DV+KMGI  YN ECR IV RY
Sbjct: 70   VGRWAHQNGHYVERRFGWDTHGLPVEYEIDKTLGISGPQDVMKMGIANYNNECRKIVMRY 129

Query: 129  VGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCKT 188
             GEWEK + R GRW+DFK+DYKT+   FMESVWW FS+L++KGLVYKG KVMP+ST C T
Sbjct: 130  SGEWEKTMGRLGRWVDFKHDYKTLYPWFMESVWWAFSELHKKGLVYKGVKVMPFSTACST 189

Query: 189  PLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCVNANFVYVK 248
            PLSNFEAGQ+YKDV DP + V F +L  P N   VAWTTTPWTLPSNLAL V+ + +YV 
Sbjct: 190  PLSNFEAGQNYKDVVDPAVFVGFKLLDCP-NRQLVAWTTTPWTLPSNLALVVHPDMLYVV 249

Query: 249  VRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTENAVEDSFE 308
             ++K +G  YVV + RL  L                                    D+ E
Sbjct: 250  TKDKTTGIEYVVLEERLGELK----------------------------------NDNLE 309

Query: 309  ILEEVLGASLVGKKYVPLFDYFNEFSDV--AFRVVADSYVTDDSGTGIVHCAPAFGEDDY 368
            ++E++ G+ L   +Y PLF YF    +   AFRV+ D++VT DSGTG+VH AP FGE D+
Sbjct: 310  VIEKLAGSQLKDLRYEPLFPYFAYMREERNAFRVLNDTFVTSDSGTGVVHQAPYFGEIDF 369

Query: 369  RVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSF 428
            +VC+ N VI K + +I  VD+ G +T+++ D+ G YVKDADK II+ +K  G LV+    
Sbjct: 370  QVCVANGVIAKDQKMICPVDESGKYTSEVPDYQGVYVKDADKLIIKRLKEMGNLVRQAEV 429

Query: 429  THSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCD---------KEKRFHNWLENAR 488
             HSYPFCWRSDTPL+Y+AVPSW +++ +++  ++   D         KEKRF NWL +AR
Sbjct: 430  KHSYPFCWRSDTPLLYKAVPSWFINVETLIPRLLANNDETYWVPAFVKEKRFANWLRDAR 489

Query: 489  DWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHITIPSRRG 548
            DWAVSR+RFWGTP+ +WVSEDGEE++ + SI +LE+LSG K+ DLHR  +D +TIPSR G
Sbjct: 490  DWAVSRNRFWGTPINLWVSEDGEEVVCVGSIAELEELSGQKITDLHRESVDDVTIPSRSG 549

Query: 549  LEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFYTL 608
               GVL+RV +VFDCWFESGSMPYA  HYPFEN ++FE NFP  F+AEG+DQTRGWFYTL
Sbjct: 550  --RGVLKRVSEVFDCWFESGSMPYAQNHYPFENRKIFEDNFPADFIAEGIDQTRGWFYTL 609

Query: 609  MVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLINSP 668
            +VLSTALF KP F+NLICNGLVLA DG KMSK  KNYP PM I+N YGADALRLYLINSP
Sbjct: 610  LVLSTALFNKPPFKNLICNGLVLASDGAKMSKSKKNYPDPMLIVNKYGADALRLYLINSP 669

Query: 669  VVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKSFN 728
            VVR E LRF++EGV  +++DVFLPW+NAYRF VQN +  E E    F   D      S N
Sbjct: 670  VVRGENLRFREEGVRDLLKDVFLPWFNAYRFFVQNVQAYEHETGNVF---DMNVHVASEN 729

Query: 729  VLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEED 788
            V+D+WI S T SLV FVRKEMD YRLY VV  L KF D LTNIY+R NRKR+KG  G  +
Sbjct: 730  VMDRWIESFTNSLVAFVRKEMDSYRLYAVVGPLTKFFDTLTNIYIRLNRKRVKGDNGLHE 789

Query: 789  CRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQ-EGGKRGE 848
               AL+ L  VL+   ++MAPFTPFF E ++ NL+KV   +EES+H+   P+ +     E
Sbjct: 790  QHHALAALGRVLILIVRLMAPFTPFFCEYIWLNLKKVIGSTEESVHFLMLPKPDESLIDE 849

Query: 849  RIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYVLEELN 908
             +E  V  M  +IDL R +R+R    +K PL+EMIV++ D  FL+D+   L  Y+L ELN
Sbjct: 850  TVERRVEVMRNVIDLVRLVRDREGLAVKYPLKEMIVINRDSQFLEDVK-SLESYILLELN 909

Query: 909  IRSLIPCDDTLKYA-SLRAEPDFSVLGKRLGKAMGVVAKEVK-AMSQEDILAFEKVGEVT 968
            +R L    D  KY  +L+AEP+F +LG RL      VA  +K  +++ ++  F   G++T
Sbjct: 910  VRKLTVSQDKQKYGITLKAEPNFKILGARLKGEQKKVADYLKNQITESELEKFLLEGKLT 969

Query: 969  IATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNR 1028
            +  H L   E+ V        G   K      D   +V++D   DESL E G+ RE+ NR
Sbjct: 970  VLGHELSSEEVAVSYAAGSDQGHGYK---THSDAKTIVMIDTTEDESLIEEGLCREVTNR 1029

Query: 1029 IQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPL 1064
            +Q+LRK+A L  TDT  V+      D+ +A +V+ A+   I  A G+P+
Sbjct: 1030 VQRLRKQAKLVSTDTAHVHIVVSPADSQLA-KVVAAKLNDIVSATGTPI 1033

BLAST of Lsi07G000360 vs. ExPASy TrEMBL
Match: A0A5A7TMQ4 (Isoleucyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003320 PE=3 SV=1)

HSP 1 Score: 2307.3 bits (5978), Expect = 0.0e+00
Identity = 1137/1181 (96.27%), Postives = 1158/1181 (98.05%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNAND INKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDGINKSNPKKKGSSNSKTE 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            N+VEDSFEILEEVLGASLVGKKYVPLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCFT+KITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKS------VICFCDKEKRFHNWLE 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSWQ    ++L++      V  F  KEKRFHNWLE
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWQKLKENLLENNEKTYWVPDFV-KEKRFHNWLE 480

Query: 481  NARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHITIPS 540
            NARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVKVFDLHRHKIDHITIPS
Sbjct: 481  NARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHITIPS 540

Query: 541  RRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWF 600
            RRG EYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWF
Sbjct: 541  RRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWF 600

Query: 601  YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLI 660
            YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLI
Sbjct: 601  YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLI 660

Query: 661  NSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQK 720
            NSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQK
Sbjct: 661  NSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQK 720

Query: 721  SFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTG 780
            SFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTG
Sbjct: 721  SFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTG 780

Query: 781  EEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEGGKR 840
            EEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEGGKR
Sbjct: 781  EEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEGGKR 840

Query: 841  GERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYVLEE 900
            GERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYVLEE
Sbjct: 841  GERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYVLEE 900

Query: 901  LNIRSLIPCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKVGEVT 960
            LNIRSLIPC+DTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQEDILAFEKVGE+T
Sbjct: 901  LNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVGEIT 960

Query: 961  IATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNR 1020
            IATHCL+LSEIKVVRDF+RPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNR
Sbjct: 961  IATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNR 1020

Query: 1021 IQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPLLPSTVLPSYAV 1080
            IQKLRKKAALEPTDTVEVYF+S DE+TSVA RVL AQ+LYISEAIGSPLLPSTVLPSYAV
Sbjct: 1021 IQKLRKKAALEPTDTVEVYFRSQDENTSVAHRVLQAQDLYISEAIGSPLLPSTVLPSYAV 1080

Query: 1081 TLAEESFHNVAGISFSIILARPVPMFNSAAITALYEGNEKFARALEVYLLSRDLSNLKSE 1140
            TLAEESFHNVAGISFSIILARP P+F+S AITALYEGNEKFARALEVYLLSRDLSNLKSE
Sbjct: 1081 TLAEESFHNVAGISFSIILARPAPVFDSDAITALYEGNEKFARALEVYLLSRDLSNLKSE 1140

Query: 1141 FSSGNGKIRVDFIEGQPIVEVLLGDHVFLTAGDQFLKANSS 1176
            FSSGNGKIRVDFI+GQP VEV+LG+HVFLTAGDQ+LKANSS
Sbjct: 1141 FSSGNGKIRVDFIDGQPSVEVVLGEHVFLTAGDQYLKANSS 1180

BLAST of Lsi07G000360 vs. ExPASy TrEMBL
Match: A0A1S3BX29 (Isoleucyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103494549 PE=3 SV=1)

HSP 1 Score: 2305.8 bits (5974), Expect = 0.0e+00
Identity = 1134/1184 (95.78%), Postives = 1157/1184 (97.72%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNAND INKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDGINKSNPKKKGSSNSKTE 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            N+VEDSFEILEEVLGASLVGKKYVPLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCFT+KITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCD---------KEKRFHN 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSW V +  + ++++   +         KEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHIT 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVKVFDLHRHKIDHIT
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  IPSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
            IPSRRG EYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
            YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  LEELNIRSLIPCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960
            LEELNIRSLIPC+DTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            E+TIATHCL+LSEIKVVRDF+RPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EITIATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

Query: 1021 VNRIQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPLLPSTVLPS 1080
            VNRIQKLRKKAALEPTDTVEVYF+S DEDTSVA RVL AQ+LYISEAIGS LLPSTVLPS
Sbjct: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAHRVLQAQDLYISEAIGSXLLPSTVLPS 1080

Query: 1081 YAVTLAEESFHNVAGISFSIILARPVPMFNSAAITALYEGNEKFARALEVYLLSRDLSNL 1140
            YAVTLAEESFHNVAGISFSIILARP P+F+S AITALYEGNEKFARALEVYLLSRDLSNL
Sbjct: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFDSDAITALYEGNEKFARALEVYLLSRDLSNL 1140

Query: 1141 KSEFSSGNGKIRVDFIEGQPIVEVLLGDHVFLTAGDQFLKANSS 1176
            KSEFSSGNGKIRVDFI+GQP VEV+LG+HVFLTAGDQ+LKANSS
Sbjct: 1141 KSEFSSGNGKIRVDFIDGQPSVEVVLGEHVFLTAGDQYLKANSS 1184

BLAST of Lsi07G000360 vs. ExPASy TrEMBL
Match: A0A0A0KTX5 (Isoleucyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G013540 PE=3 SV=1)

HSP 1 Score: 2304.2 bits (5970), Expect = 0.0e+00
Identity = 1134/1184 (95.78%), Postives = 1156/1184 (97.64%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLY+KGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNA NAND INKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            N+VEDSFEILEEVLGASLVGKKYVPLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCF +KITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCD---------KEKRFHN 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSW V +  + ++++   +         KEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHIT 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVKVFDLHRHKIDHIT
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  IPSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
            IPSRRG EYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
            YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  LEELNIRSLIPCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960
            LEELNIRSLIPC+DTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            EVTIATHCLKLSEIKVVRDF+RPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

Query: 1021 VNRIQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPLLPSTVLPS 1080
            VNRIQKLRKKAALEPTDTVEVYF+S DEDTSVAQRVL AQELYISEAIGSPLLPSTVLPS
Sbjct: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS 1080

Query: 1081 YAVTLAEESFHNVAGISFSIILARPVPMFNSAAITALYEGNEKFARALEVYLLSRDLSNL 1140
            YAVTLAEESFHNVAGISFSIILARP P+FNS AITALYEGNEKFAR+LEVYLLSRDLSNL
Sbjct: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL 1140

Query: 1141 KSEFSSGNGKIRVDFIEGQPIVEVLLGDHVFLTAGDQFLKANSS 1176
            KSEFS+GNGKIRVDFI+GQP VEV+L +HVFLTAGDQ+LKANSS
Sbjct: 1141 KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1184

BLAST of Lsi07G000360 vs. ExPASy TrEMBL
Match: A0A6J1GTY1 (Isoleucyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457524 PE=3 SV=1)

HSP 1 Score: 2258.8 bits (5852), Expect = 0.0e+00
Identity = 1111/1185 (93.76%), Postives = 1145/1185 (96.62%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEE VLEYWSDI+AFETQLERT+DLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEVVLEYWSDIRAFETQLERTKDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQL+EKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLFEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMV FPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPN-ANDAINKSNPKKKGSSNSKT 300
            NANFVYVKVRNK SGKVYVVAKSRLSALPVEKQKVNAPN + + +NKSNPKKKGSSNSKT
Sbjct: 241  NANFVYVKVRNKASGKVYVVAKSRLSALPVEKQKVNAPNGSTEVVNKSNPKKKGSSNSKT 300

Query: 301  ENAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            EN+VEDS+E+LEEVLGASLVGKKY PLFDYF EFSDVAFRVVAD+YVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSYEVLEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADNYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGR 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKIT FSGRYVKDADKDIIEAVKAQGR
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITQFSGRYVKDADKDIIEAVKAQGR 420

Query: 421  LVKSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCD---------KEKRFH 480
            LVKSGSFTHSYPFCWRSDTPLIYRAVPSW V +  + + ++   +         KEKRFH
Sbjct: 421  LVKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKEKLLENNEKTYWVPDFVKEKRFH 480

Query: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHI 540
            NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHI
Sbjct: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHI 540

Query: 541  TIPSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600
            TIPSRRG EYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT
Sbjct: 541  TIPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600

Query: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR 660
            RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR
Sbjct: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR 660

Query: 661  LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA 720
            LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA
Sbjct: 661  LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA 720

Query: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780
            TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK
Sbjct: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780

Query: 781  GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE 840
            GRTGEEDCRIALSTLYHVLLTSC VMAPFTPFFTE LYQNLRKVSDGSEESIHYCSYPQE
Sbjct: 781  GRTGEEDCRIALSTLYHVLLTSCMVMAPFTPFFTEALYQNLRKVSDGSEESIHYCSYPQE 840

Query: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREY 900
            GGKRGERIEESVTRMMTIIDLARNIRERHNKPLK PLREMIVVHPDKDFLDDIAGKLREY
Sbjct: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDDIAGKLREY 900

Query: 901  VLEELNIRSLIPCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKV 960
            VLEELNIRSLIPC+DTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKV
Sbjct: 901  VLEELNIRSLIPCNDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKV 960

Query: 961  GEVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVARE 1020
            GEVTIATHCL+LS+IKVVRDF+RPDGMTDKE+DAAGDGDVLVILDLRPDESLFEAGVARE
Sbjct: 961  GEVTIATHCLRLSDIKVVRDFKRPDGMTDKEMDAAGDGDVLVILDLRPDESLFEAGVARE 1020

Query: 1021 IVNRIQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPLLPSTVLP 1080
            IVNRIQKLRKKAALEPTD+VEVYF+S DED SVAQRVLNAQELYISEAIGSPLLPS +LP
Sbjct: 1021 IVNRIQKLRKKAALEPTDSVEVYFRSQDEDASVAQRVLNAQELYISEAIGSPLLPSALLP 1080

Query: 1081 SYAVTLAEESFHNVAGISFSIILARPVPMFNSAAITALYEGNEKFARALEVYLLSRDLSN 1140
            S+AVTLAEESF NVAGISFSIILARPVP+FNS A+TALY+GNEKFARALEVYLLSRDLSN
Sbjct: 1081 SHAVTLAEESFQNVAGISFSIILARPVPVFNSDAVTALYKGNEKFARALEVYLLSRDLSN 1140

Query: 1141 LKSEFSSGNGKIRVDFIEGQPIVEVLLGDHVFLTAGDQFLKANSS 1176
            LK+EF SGNGK+RVD+I+GQP VEV LG+HVFLTAGD+FLKA SS
Sbjct: 1141 LKTEF-SGNGKMRVDYIDGQPGVEVALGEHVFLTAGDKFLKAKSS 1184

BLAST of Lsi07G000360 vs. ExPASy TrEMBL
Match: A0A6J1GH53 (Isoleucyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111454148 PE=3 SV=1)

HSP 1 Score: 2257.3 bits (5848), Expect = 0.0e+00
Identity = 1113/1185 (93.92%), Postives = 1139/1185 (96.12%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGI+KYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMV FPVL DP NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNA-NDAINKSNPKKKGSSNSKT 300
            NANF+YVKVRNKVSGKV+VVAKSRLSALPVEKQKV+APN   D   KSNPK KGSSNSKT
Sbjct: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVSAPNGPTDVSKKSNPKTKGSSNSKT 300

Query: 301  ENAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            EN+VEDSFEILEEVLGASLVGKKY PLFDYF E SDVAFRVVADSYVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEVSDVAFRVVADSYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGR 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGR
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGR 420

Query: 421  LVKSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCD---------KEKRFH 480
            LVKSGSFTHSYPFCWRSDTPLIYRAVPSW V +  + + ++   +         KEKRFH
Sbjct: 421  LVKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKEKLLENNEKTYWVPDFVKEKRFH 480

Query: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHI 540
            NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSG KVFDLHRHKIDHI
Sbjct: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDLHRHKIDHI 540

Query: 541  TIPSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600
            TIPS+RG EYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT
Sbjct: 541  TIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600

Query: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR 660
            RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR
Sbjct: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR 660

Query: 661  LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA 720
            LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEG A FSP+DQA
Sbjct: 661  LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLARFSPLDQA 720

Query: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780
            TLQKSFNVLDQWINSATQSLVYFVRKEMD YRLYTVVPYLLKFLDNLTNIYVRFNRKRLK
Sbjct: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780

Query: 781  GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE 840
            GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE
Sbjct: 781  GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE 840

Query: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREY 900
            GGKRGERIEESVTRMMTIIDLARNIRERHNKPLK PLREMIVVHPDKDFLDDIAGKLREY
Sbjct: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDDIAGKLREY 900

Query: 901  VLEELNIRSLIPCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKV 960
            VLEELNIRSLIPC+DTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQEDILAFEKV
Sbjct: 901  VLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQEDILAFEKV 960

Query: 961  GEVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVARE 1020
            G VTIATHCL+LSEIKVVRDFRRPDGMTDKEIDA+GDGDVLVILDLRPDESLFEAGVARE
Sbjct: 961  GNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESLFEAGVARE 1020

Query: 1021 IVNRIQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPLLPSTVLP 1080
            IVNRIQKLRKKAALEPTDTVEVYF+SLD+D SVA+RVL AQELYISEAIGSPLLPS VLP
Sbjct: 1021 IVNRIQKLRKKAALEPTDTVEVYFRSLDDDISVAERVLRAQELYISEAIGSPLLPSAVLP 1080

Query: 1081 SYAVTLAEESFHNVAGISFSIILARPVPMFNSAAITALYEGNEKFARALEVYLLSRDLSN 1140
            SYAVTLAEESFHNVAGISFSIILARPVP+FNS +ITALYEGNEK ARALEVY LSRDLSN
Sbjct: 1081 SYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVYFLSRDLSN 1140

Query: 1141 LKSEFSSGNGKIRVDFIEGQPIVEVLLGDHVFLTAGDQFLKANSS 1176
            LKSEFSSGNGKIRVDFI+GQP VEV+LG+HVFLTAGDQFLKA SS
Sbjct: 1141 LKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS 1185

BLAST of Lsi07G000360 vs. NCBI nr
Match: KAA0044582.1 (isoleucine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 2307.3 bits (5978), Expect = 0.0e+00
Identity = 1137/1181 (96.27%), Postives = 1158/1181 (98.05%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNAND INKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDGINKSNPKKKGSSNSKTE 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            N+VEDSFEILEEVLGASLVGKKYVPLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCFT+KITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKS------VICFCDKEKRFHNWLE 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSWQ    ++L++      V  F  KEKRFHNWLE
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWQKLKENLLENNEKTYWVPDFV-KEKRFHNWLE 480

Query: 481  NARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHITIPS 540
            NARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVKVFDLHRHKIDHITIPS
Sbjct: 481  NARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHITIPS 540

Query: 541  RRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWF 600
            RRG EYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWF
Sbjct: 541  RRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWF 600

Query: 601  YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLI 660
            YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLI
Sbjct: 601  YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLI 660

Query: 661  NSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQK 720
            NSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQK
Sbjct: 661  NSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQK 720

Query: 721  SFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTG 780
            SFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTG
Sbjct: 721  SFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTG 780

Query: 781  EEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEGGKR 840
            EEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEGGKR
Sbjct: 781  EEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEGGKR 840

Query: 841  GERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYVLEE 900
            GERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYVLEE
Sbjct: 841  GERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYVLEE 900

Query: 901  LNIRSLIPCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKVGEVT 960
            LNIRSLIPC+DTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQEDILAFEKVGE+T
Sbjct: 901  LNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVGEIT 960

Query: 961  IATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNR 1020
            IATHCL+LSEIKVVRDF+RPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNR
Sbjct: 961  IATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNR 1020

Query: 1021 IQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPLLPSTVLPSYAV 1080
            IQKLRKKAALEPTDTVEVYF+S DE+TSVA RVL AQ+LYISEAIGSPLLPSTVLPSYAV
Sbjct: 1021 IQKLRKKAALEPTDTVEVYFRSQDENTSVAHRVLQAQDLYISEAIGSPLLPSTVLPSYAV 1080

Query: 1081 TLAEESFHNVAGISFSIILARPVPMFNSAAITALYEGNEKFARALEVYLLSRDLSNLKSE 1140
            TLAEESFHNVAGISFSIILARP P+F+S AITALYEGNEKFARALEVYLLSRDLSNLKSE
Sbjct: 1081 TLAEESFHNVAGISFSIILARPAPVFDSDAITALYEGNEKFARALEVYLLSRDLSNLKSE 1140

Query: 1141 FSSGNGKIRVDFIEGQPIVEVLLGDHVFLTAGDQFLKANSS 1176
            FSSGNGKIRVDFI+GQP VEV+LG+HVFLTAGDQ+LKANSS
Sbjct: 1141 FSSGNGKIRVDFIDGQPSVEVVLGEHVFLTAGDQYLKANSS 1180

BLAST of Lsi07G000360 vs. NCBI nr
Match: XP_008453992.2 (PREDICTED: LOW QUALITY PROTEIN: isoleucine--tRNA ligase, cytoplasmic-like [Cucumis melo])

HSP 1 Score: 2305.8 bits (5974), Expect = 0.0e+00
Identity = 1134/1184 (95.78%), Postives = 1157/1184 (97.72%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNAND INKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDGINKSNPKKKGSSNSKTE 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            N+VEDSFEILEEVLGASLVGKKYVPLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCFT+KITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCD---------KEKRFHN 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSW V +  + ++++   +         KEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHIT 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVKVFDLHRHKIDHIT
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  IPSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
            IPSRRG EYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
            YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  LEELNIRSLIPCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960
            LEELNIRSLIPC+DTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            E+TIATHCL+LSEIKVVRDF+RPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EITIATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

Query: 1021 VNRIQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPLLPSTVLPS 1080
            VNRIQKLRKKAALEPTDTVEVYF+S DEDTSVA RVL AQ+LYISEAIGS LLPSTVLPS
Sbjct: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAHRVLQAQDLYISEAIGSXLLPSTVLPS 1080

Query: 1081 YAVTLAEESFHNVAGISFSIILARPVPMFNSAAITALYEGNEKFARALEVYLLSRDLSNL 1140
            YAVTLAEESFHNVAGISFSIILARP P+F+S AITALYEGNEKFARALEVYLLSRDLSNL
Sbjct: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFDSDAITALYEGNEKFARALEVYLLSRDLSNL 1140

Query: 1141 KSEFSSGNGKIRVDFIEGQPIVEVLLGDHVFLTAGDQFLKANSS 1176
            KSEFSSGNGKIRVDFI+GQP VEV+LG+HVFLTAGDQ+LKANSS
Sbjct: 1141 KSEFSSGNGKIRVDFIDGQPSVEVVLGEHVFLTAGDQYLKANSS 1184

BLAST of Lsi07G000360 vs. NCBI nr
Match: XP_004152106.1 (isoleucine--tRNA ligase, cytoplasmic [Cucumis sativus] >XP_011653047.1 isoleucine--tRNA ligase, cytoplasmic [Cucumis sativus] >XP_011653048.1 isoleucine--tRNA ligase, cytoplasmic [Cucumis sativus] >XP_031739930.1 isoleucine--tRNA ligase, cytoplasmic [Cucumis sativus] >KGN53060.1 hypothetical protein Csa_014568 [Cucumis sativus])

HSP 1 Score: 2304.2 bits (5970), Expect = 0.0e+00
Identity = 1134/1184 (95.78%), Postives = 1156/1184 (97.64%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLY+KGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNA NAND INKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            N+VEDSFEILEEVLGASLVGKKYVPLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCF +KITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCD---------KEKRFHN 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSW V +  + ++++   +         KEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHIT 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVKVFDLHRHKIDHIT
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  IPSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
            IPSRRG EYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
            YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  LEELNIRSLIPCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960
            LEELNIRSLIPC+DTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            EVTIATHCLKLSEIKVVRDF+RPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

Query: 1021 VNRIQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPLLPSTVLPS 1080
            VNRIQKLRKKAALEPTDTVEVYF+S DEDTSVAQRVL AQELYISEAIGSPLLPSTVLPS
Sbjct: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS 1080

Query: 1081 YAVTLAEESFHNVAGISFSIILARPVPMFNSAAITALYEGNEKFARALEVYLLSRDLSNL 1140
            YAVTLAEESFHNVAGISFSIILARP P+FNS AITALYEGNEKFAR+LEVYLLSRDLSNL
Sbjct: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL 1140

Query: 1141 KSEFSSGNGKIRVDFIEGQPIVEVLLGDHVFLTAGDQFLKANSS 1176
            KSEFS+GNGKIRVDFI+GQP VEV+L +HVFLTAGDQ+LKANSS
Sbjct: 1141 KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1184

BLAST of Lsi07G000360 vs. NCBI nr
Match: XP_038898074.1 (isoleucine--tRNA ligase, cytoplasmic isoform X2 [Benincasa hispida])

HSP 1 Score: 2293.5 bits (5942), Expect = 0.0e+00
Identity = 1134/1184 (95.78%), Postives = 1150/1184 (97.13%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMV FPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNAND++ KSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDSV-KSNPKKKGSSNSKTE 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            N+VEDSFEILEEVLGASLVGKKYVPLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCD---------KEKRFHN 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSW V +  + + ++   +         KEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKEKLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHIT 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVKVFDLHRHKIDHIT
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  IPSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
            IPS+RG EYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVE FEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVEHFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
            YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVH DKDFLDDIAGKLREYV
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHSDKDFLDDIAGKLREYV 900

Query: 901  LEELNIRSLIPCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960
            LEELNIRSLI CDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLITCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            EVTIATHCL+LSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

Query: 1021 VNRIQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPLLPSTVLPS 1080
            VNRIQKLRKKAALEPTDTVEVYFKS DED SVAQRVL AQELYISEAIGSPLLPSTVLPS
Sbjct: 1021 VNRIQKLRKKAALEPTDTVEVYFKSQDEDISVAQRVLRAQELYISEAIGSPLLPSTVLPS 1080

Query: 1081 YAVTLAEESFHNVAGISFSIILARPVPMFNSAAITALYEGNEKFARALEVYLLSRDLSNL 1140
            YAVTLAEESFHNVAGISFSIILARP   FNS AITALYEGNEKFARALEVYLLSRDL+NL
Sbjct: 1081 YAVTLAEESFHNVAGISFSIILARPALAFNSDAITALYEGNEKFARALEVYLLSRDLTNL 1140

Query: 1141 KSEFSSGNGKIRVDFIEGQPIVEVLLGDHVFLTAGDQFLKANSS 1176
            KSEFSSGNGKIRVDFI+GQP VEVLLG+HVFLTAGDQFLKA +S
Sbjct: 1141 KSEFSSGNGKIRVDFIDGQPSVEVLLGEHVFLTAGDQFLKAKAS 1183

BLAST of Lsi07G000360 vs. NCBI nr
Match: XP_023537189.1 (isoleucine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2262.6 bits (5862), Expect = 0.0e+00
Identity = 1115/1185 (94.09%), Postives = 1141/1185 (96.29%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGI+KYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMV FPVL DP NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNA-NDAINKSNPKKKGSSNSKT 300
            NANF+YVKVRNKVSGKV+VVAKSRLSALPVEKQKVNAPN   D   KSNPK KGSSNSKT
Sbjct: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300

Query: 301  ENAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            EN+VEDSFEILEEVLGASLVGKKY PLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGR 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGR
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGR 420

Query: 421  LVKSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCD---------KEKRFH 480
            LVKSGSFTHSYPFCWRSDTPLIYRAVPSW V +  + + ++   +         KEKRFH
Sbjct: 421  LVKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKEKLLENNEKTYWVPDFVKEKRFH 480

Query: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHI 540
            NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSG KVFDLHRHKIDHI
Sbjct: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDLHRHKIDHI 540

Query: 541  TIPSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600
            TIPS+RG EYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT
Sbjct: 541  TIPSQRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600

Query: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR 660
            RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEI+NDYGADALR
Sbjct: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVNDYGADALR 660

Query: 661  LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA 720
            LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEG A FSP+DQA
Sbjct: 661  LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLAQFSPLDQA 720

Query: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780
            TLQKSFNVLDQWINSATQSLVYFVRKEMD YRLYTVVPYLLKFLDNLTNIYVRFNRKRLK
Sbjct: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780

Query: 781  GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE 840
            GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE
Sbjct: 781  GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE 840

Query: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREY 900
            GGKRGERIEESVTRMMTIIDLARNIRERHNKPLK PLREMIVVHPDKDFLDDIAGKLREY
Sbjct: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDDIAGKLREY 900

Query: 901  VLEELNIRSLIPCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKV 960
            VLEELNIRSLIPC+DTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQEDILAFEKV
Sbjct: 901  VLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQEDILAFEKV 960

Query: 961  GEVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVARE 1020
            G VTIATHCL+LSEIKVVRDFRRPDGMTDKEIDA+GDGDVLVILDLRPDESLFEAGVARE
Sbjct: 961  GNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESLFEAGVARE 1020

Query: 1021 IVNRIQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPLLPSTVLP 1080
            IVNRIQKLRKKAALEPTDTVEVYF+SLD+D SVA+RVL AQELYISEAIGSPLLPS VLP
Sbjct: 1021 IVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSPLLPSAVLP 1080

Query: 1081 SYAVTLAEESFHNVAGISFSIILARPVPMFNSAAITALYEGNEKFARALEVYLLSRDLSN 1140
            SYAVTLAEESFHNVAGISFSIILARPVP+FNS +ITALYEGNEK ARALEVYLLSRDLSN
Sbjct: 1081 SYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVYLLSRDLSN 1140

Query: 1141 LKSEFSSGNGKIRVDFIEGQPIVEVLLGDHVFLTAGDQFLKANSS 1176
            LKSEFSSGNGKIRVDFI+GQP VEV+LG+HVFLTAGDQFLKA SS
Sbjct: 1141 LKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS 1185

BLAST of Lsi07G000360 vs. TAIR 10
Match: AT4G10320.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 1852.8 bits (4798), Expect = 0.0e+00
Identity = 905/1196 (75.67%), Postives = 1026/1196 (85.79%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            M+EVCEGK+FSFP+ EE VL +W++I AF+TQL+RT +LPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MEEVCEGKEFSFPRQEEDVLSFWTEIDAFKTQLKRTENLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQ MTGHHVTRRFGWDCHGLPVENEID+KL IKRRD+V+KMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQTMTGHHVTRRFGWDCHGLPVENEIDRKLNIKRRDEVIKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYV EWEK+ITR GRWIDFKNDYKTMDL FMESVWWVFSQL+EK LVY+GFKVM
Sbjct: 121  CRSIVTRYVAEWEKVITRCGRWIDFKNDYKTMDLPFMESVWWVFSQLWEKNLVYRGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQ+YK+VPDPEIMV FPV+GD  NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQNYKEVPDPEIMVTFPVIGDQDNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPN--ANDAINKSNPKKKGSSNSK 300
            NA FVYVKVRNK +GKVY+VA+SRLSALP +K K    N  A D   K+NPK KG   +K
Sbjct: 241  NAKFVYVKVRNKNTGKVYIVAESRLSALPTDKPKAKLSNGPAGDT-KKANPKAKG---AK 300

Query: 301  TENAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCA 360
             E+A  DS+E+LE+  GASLVGKKY PLFDYF++FS  AFRVVAD YVTDDSGTGIVHCA
Sbjct: 301  PESAA-DSYEVLEKFNGASLVGKKYEPLFDYFSDFSSEAFRVVADDYVTDDSGTGIVHCA 360

Query: 361  PAFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQG 420
            PAFGEDDYRVC+ N++I KGENL+VAVDDDG FT +IT FSGRYVKDADKDIIEAVKA+G
Sbjct: 361  PAFGEDDYRVCLLNKIIKKGENLVVAVDDDGLFTERITHFSGRYVKDADKDIIEAVKAKG 420

Query: 421  RLVKSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCD---------KEKRF 480
            RLVK+GSFTHSYPFCWRSDTPLIYRAVPSW V +  + + ++   +         K+KRF
Sbjct: 421  RLVKTGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEQLKEKLLKSNEQTEWVPGYVKDKRF 480

Query: 481  HNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDH 540
            HNWLENARDWA+SRSRFWGTPLP+W+S+DGEE+++MDS+EKLE+LSGVKVFDLHRH IDH
Sbjct: 481  HNWLENARDWAISRSRFWGTPLPIWISDDGEEVVIMDSVEKLEKLSGVKVFDLHRHHIDH 540

Query: 541  ITIPSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQ 600
            ITIPS RG E+GVLRRV+DVFDCWFESGSMPYAYIHYPFEN ELFEKNFPG FVAEGLDQ
Sbjct: 541  ITIPSSRGDEFGVLRRVEDVFDCWFESGSMPYAYIHYPFENKELFEKNFPGDFVAEGLDQ 600

Query: 601  TRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADAL 660
            TRGWFYTLMVLSTALF KPAF+NLICNGLVLAEDGKKM+K+L+NYP P+E+I++YGADA+
Sbjct: 601  TRGWFYTLMVLSTALFEKPAFKNLICNGLVLAEDGKKMAKKLRNYPPPLEVIDEYGADAV 660

Query: 661  RLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQ 720
            RLYLINSPVVRAE LRFKKEGV GVV+DVFLPWYNAYRFLVQNAKRLE EG  PF P D 
Sbjct: 661  RLYLINSPVVRAEPLRFKKEGVLGVVKDVFLPWYNAYRFLVQNAKRLETEGGVPFVPTDL 720

Query: 721  ATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRL 780
            AT+Q S N+LDQWI+SATQSLV FVR+EMD YRLYTVVP LLKFLDNLTNIYVRFNRKRL
Sbjct: 721  ATIQ-SANILDQWIHSATQSLVRFVREEMDAYRLYTVVPRLLKFLDNLTNIYVRFNRKRL 780

Query: 781  KGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQ 840
            KGRTGE+DC  ALSTL++VLLTSCKVMAPFTPFFTE LYQNLRK   GSEES+HYCS P 
Sbjct: 781  KGRTGEDDCHTALSTLFNVLLTSCKVMAPFTPFFTETLYQNLRKACKGSEESVHYCSIPP 840

Query: 841  EGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLRE 900
              G  GERIE SVTRMM IIDLARNIRER+  PLK PL+EMIVVHPD DFL+DI G LRE
Sbjct: 841  REGMEGERIELSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADFLNDITGVLRE 900

Query: 901  YVLEELNIRSLIPCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEK 960
            YVLEELN+RSL+PC+DTLKYASL+AEPDFSVLGKRLGK+MG+VAKEVK MSQ+DILAFE+
Sbjct: 901  YVLEELNVRSLVPCNDTLKYASLKAEPDFSVLGKRLGKSMGLVAKEVKEMSQKDILAFEE 960

Query: 961  VGEVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAR 1020
             GEVTIA H LK ++IK+VR F+RPD + + EID+AGDGDVLVILDLR D+SL EAG AR
Sbjct: 961  AGEVTIANHLLKETDIKIVRVFKRPDDLKENEIDSAGDGDVLVILDLRADDSLVEAGFAR 1020

Query: 1021 EIVNRIQKLRKKAALEPTDTVEVYFKSLDEDTSVAQRVLNAQELYISEAIGSPLLPSTVL 1080
            EIVNRIQKLRKK+ LEPTD VEVYF+SLDED SV+++VL +QE  I ++IGS LL S+++
Sbjct: 1021 EIVNRIQKLRKKSGLEPTDFVEVYFQSLDEDESVSKQVLVSQEQNIKDSIGSTLLLSSLM 1080

Query: 1081 PSYAVTLAEESF----------HNVAGISFSIILARPVPMFNSAAITALYEGNEKFARAL 1140
            PS+AV +A+E+F            V  +S+ I LARP   FN  A+ ALY G+ K A  L
Sbjct: 1081 PSHAVIIADETFTPKETSDESVKKVPKLSYKISLARPALKFNEEAVLALYSGDVKSATGL 1140

Query: 1141 EVYLLSRDLSNLKSEFSSGNGKIRVDFIEGQPIVEVLLGDHVFLTAGDQFLKANSS 1176
            + YLLSRD SNLKSEF +G+GKI V  IE  P+  V+LG+H+ L+ GD  L   ++
Sbjct: 1141 QTYLLSRDHSNLKSEFQAGDGKITVSCIENVPVATVVLGEHLHLSVGDDLLSKRNA 1190

BLAST of Lsi07G000360 vs. TAIR 10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 289.3 bits (739), Expect = 1.4e-77
Identity = 230/887 (25.93%), Postives = 387/887 (43.63%), Query Frame = 0

Query: 16  EEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQVM 75
           E  + + W + + F+ ++    +   +I +DGPP+A G  H GH L   +KDI+ RY+++
Sbjct: 123 EPELQKLWEENQVFK-RVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLL 182

Query: 76  TGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTRYVGEWEKI 135
             + V    GWDCHGLP+E ++ Q L  + R ++  +   K   +        V    + 
Sbjct: 183 QNYKVQYVPGWDCHGLPIELKVLQSLDQEVRKELTPL---KLRAKAAKFAKATVKTQMES 242

Query: 136 ITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCKTPLSNFEA 195
             R G W D+ N Y T+D ++  +   VF Q+  KG +Y+G K + +S   +T L+  E 
Sbjct: 243 FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 302

Query: 196 GQSYKDVPDPEIMVAFPVLGDPQ---------NAAFVAWTTTPWTLPSNLALCVNANFVY 255
                 +    I   F ++G  +         N     WTTTPWT+P+N A+ VNA   Y
Sbjct: 303 EYPEGHI-SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 362

Query: 256 VKVRNKVSGKVYVVAKSRLSALP----VEKQKVNAPNANDAINKSNPKKKGSSNSKTENA 315
             V  +   +      S    +P      +QK+    A D +    P  +     K    
Sbjct: 363 SVVEVQSFSEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLV----PALEAKWGVK---- 422

Query: 316 VEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPAFG 375
                 I +  LG+ L   +Y    D  N    V   V+   Y+T +SGTG+VH AP  G
Sbjct: 423 ----LSISKTFLGSDLENCRYTHPID--NRDCPV---VIGGDYITTESGTGLVHTAPGHG 482

Query: 376 EDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYV-KDADKDIIEAVKAQGRLV 435
           ++DY   ++      G  L+  VDD+G FT +   F G  V  + +  ++  +     LV
Sbjct: 483 QEDYATGLK-----YGLPLVSPVDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLV 542

Query: 436 KSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCDKEK--------RFHNWL 495
              S+ H YP+ WR+  P I+RA   W   +     + +   +  K        R     
Sbjct: 543 MEESYAHKYPYDWRTKKPTIFRATEQWFASVEGFRTATMDAINNVKWVPHQAVNRISAMT 602

Query: 496 ENARDWAVSRSRFWGTPLPVWVS-EDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHITI 555
            +  DW +SR R WG P+P +   +  E ++  ++I  ++ +   K  D   +      +
Sbjct: 603 SSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLL 662

Query: 556 PSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRG 615
           P +   +     +  D  D WF+SGS  +A +    E +     +FP     EG DQ RG
Sbjct: 663 PEKYRDKAADYEKGTDTMDVWFDSGS-SWAGVLGKREGL-----SFPADVYLEGTDQHRG 722

Query: 616 WFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIND--------- 675
           WF + ++ S A  GK  +  +I +G VL E G KMSK L N   P  +I           
Sbjct: 723 WFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPA 782

Query: 676 YGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAP 735
           YGAD +RL+ ++S     + L   +  +   + D++       R+L+ N     ++   P
Sbjct: 783 YGADVMRLW-VSSVDYTGDVLIGPQ--ILRQMSDIYRKLRGTLRYLLGNLHDWRVDNAVP 842

Query: 736 FSPVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKF-LDNLTNIYV 795
           +         +   ++DQ      +++V  +++  + Y+ + +   + +F + +L+N Y 
Sbjct: 843 Y---------QDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYF 902

Query: 796 RFNRKRL-KGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNL---RKVSDGS 855
              + RL  G T     R   + L   LL+  +V+AP  P   E ++QNL    +  DGS
Sbjct: 903 DIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDGS 962

BLAST of Lsi07G000360 vs. TAIR 10
Match: AT5G49030.3 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 289.3 bits (739), Expect = 1.4e-77
Identity = 230/887 (25.93%), Postives = 387/887 (43.63%), Query Frame = 0

Query: 16   EEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQVM 75
            E  + + W + + F+ ++    +   +I +DGPP+A G  H GH L   +KDI+ RY+++
Sbjct: 309  EPELQKLWEENQVFK-RVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLL 368

Query: 76   TGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTRYVGEWEKI 135
              + V    GWDCHGLP+E ++ Q L  + R ++  +   K   +        V    + 
Sbjct: 369  QNYKVQYVPGWDCHGLPIELKVLQSLDQEVRKELTPL---KLRAKAAKFAKATVKTQMES 428

Query: 136  ITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCKTPLSNFEA 195
              R G W D+ N Y T+D ++  +   VF Q+  KG +Y+G K + +S   +T L+  E 
Sbjct: 429  FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 488

Query: 196  GQSYKDVPDPEIMVAFPVLGDPQ---------NAAFVAWTTTPWTLPSNLALCVNANFVY 255
                  +    I   F ++G  +         N     WTTTPWT+P+N A+ VNA   Y
Sbjct: 489  EYPEGHI-SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 548

Query: 256  VKVRNKVSGKVYVVAKSRLSALP----VEKQKVNAPNANDAINKSNPKKKGSSNSKTENA 315
              V  +   +      S    +P      +QK+    A D +    P  +     K    
Sbjct: 549  SVVEVQSFSEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLV----PALEAKWGVK---- 608

Query: 316  VEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPAFG 375
                  I +  LG+ L   +Y    D  N    V   V+   Y+T +SGTG+VH AP  G
Sbjct: 609  ----LSISKTFLGSDLENCRYTHPID--NRDCPV---VIGGDYITTESGTGLVHTAPGHG 668

Query: 376  EDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYV-KDADKDIIEAVKAQGRLV 435
            ++DY   ++      G  L+  VDD+G FT +   F G  V  + +  ++  +     LV
Sbjct: 669  QEDYATGLK-----YGLPLVSPVDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLV 728

Query: 436  KSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCDKEK--------RFHNWL 495
               S+ H YP+ WR+  P I+RA   W   +     + +   +  K        R     
Sbjct: 729  MEESYAHKYPYDWRTKKPTIFRATEQWFASVEGFRTATMDAINNVKWVPHQAVNRISAMT 788

Query: 496  ENARDWAVSRSRFWGTPLPVWVS-EDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHITI 555
             +  DW +SR R WG P+P +   +  E ++  ++I  ++ +   K  D   +      +
Sbjct: 789  SSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLL 848

Query: 556  PSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRG 615
            P +   +     +  D  D WF+SGS  +A +    E +     +FP     EG DQ RG
Sbjct: 849  PEKYRDKAADYEKGTDTMDVWFDSGS-SWAGVLGKREGL-----SFPADVYLEGTDQHRG 908

Query: 616  WFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIND--------- 675
            WF + ++ S A  GK  +  +I +G VL E G KMSK L N   P  +I           
Sbjct: 909  WFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPA 968

Query: 676  YGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAP 735
            YGAD +RL+ ++S     + L   +  +   + D++       R+L+ N     ++   P
Sbjct: 969  YGADVMRLW-VSSVDYTGDVLIGPQ--ILRQMSDIYRKLRGTLRYLLGNLHDWRVDNAVP 1028

Query: 736  FSPVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKF-LDNLTNIYV 795
            +         +   ++DQ      +++V  +++  + Y+ + +   + +F + +L+N Y 
Sbjct: 1029 Y---------QDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYF 1088

Query: 796  RFNRKRL-KGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNL---RKVSDGS 855
               + RL  G T     R   + L   LL+  +V+AP  P   E ++QNL    +  DGS
Sbjct: 1089 DIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDGS 1148

BLAST of Lsi07G000360 vs. TAIR 10
Match: AT5G49030.2 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 282.0 bits (720), Expect = 2.3e-75
Identity = 217/830 (26.14%), Postives = 365/830 (43.98%), Query Frame = 0

Query: 16  EEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQVM 75
           E  + + W + + F+ ++    +   +I +DGPP+A G  H GH L   +KDI+ RY+++
Sbjct: 123 EPELQKLWEENQVFK-RVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLL 182

Query: 76  TGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTRYVGEWEKI 135
             + V    GWDCHGLP+E ++ Q L  + R ++  +   K   +        V    + 
Sbjct: 183 QNYKVQYVPGWDCHGLPIELKVLQSLDQEVRKELTPL---KLRAKAAKFAKATVKTQMES 242

Query: 136 ITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCKTPLSNFEA 195
             R G W D+ N Y T+D ++  +   VF Q+  KG +Y+G K + +S   +T L+  E 
Sbjct: 243 FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 302

Query: 196 GQSYKDVPDPEIMVAFPVLGDPQ---------NAAFVAWTTTPWTLPSNLALCVNANFVY 255
                 +    I   F ++G  +         N     WTTTPWT+P+N A+ VNA   Y
Sbjct: 303 EYPEGHI-SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 362

Query: 256 VKVRNKVSGKVYVVAKSRLSALP----VEKQKVNAPNANDAINKSNPKKKGSSNSKTENA 315
             V  +   +      S    +P      +QK+    A D +    P  +     K    
Sbjct: 363 SVVEVQSFSEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLV----PALEAKWGVK---- 422

Query: 316 VEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPAFG 375
                 I +  LG+ L   +Y    D  N    V   V+   Y+T +SGTG+VH AP  G
Sbjct: 423 ----LSISKTFLGSDLENCRYTHPID--NRDCPV---VIGGDYITTESGTGLVHTAPGHG 482

Query: 376 EDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYV-KDADKDIIEAVKAQGRLV 435
           ++DY   ++      G  L+  VDD+G FT +   F G  V  + +  ++  +     LV
Sbjct: 483 QEDYATGLK-----YGLPLVSPVDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLV 542

Query: 436 KSGSFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCDKEK--------RFHNWL 495
              S+ H YP+ WR+  P I+RA   W   +     + +   +  K        R     
Sbjct: 543 MEESYAHKYPYDWRTKKPTIFRATEQWFASVEGFRTATMDAINNVKWVPHQAVNRISAMT 602

Query: 496 ENARDWAVSRSRFWGTPLPVWVS-EDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHITI 555
            +  DW +SR R WG P+P +   +  E ++  ++I  ++ +   K  D   +      +
Sbjct: 603 SSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLL 662

Query: 556 PSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRG 615
           P +   +     +  D  D WF+SGS  +A +    E +     +FP     EG DQ RG
Sbjct: 663 PEKYRDKAADYEKGTDTMDVWFDSGS-SWAGVLGKREGL-----SFPADVYLEGTDQHRG 722

Query: 616 WFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIND--------- 675
           WF + ++ S A  GK  +  +I +G VL E G KMSK L N   P  +I           
Sbjct: 723 WFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPA 782

Query: 676 YGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAP 735
           YGAD +RL+ ++S     + L   +  +   + D++       R+L+ N     ++   P
Sbjct: 783 YGADVMRLW-VSSVDYTGDVLIGPQ--ILRQMSDIYRKLRGTLRYLLGNLHDWRVDNAVP 842

Query: 736 FSPVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKF-LDNLTNIYV 795
           +         +   ++DQ      +++V  +++  + Y+ + +   + +F + +L+N Y 
Sbjct: 843 Y---------QDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYF 902

Query: 796 RFNRKRL-KGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNL 812
              + RL  G T     R   + L   LL+  +V+AP  P   E ++QNL
Sbjct: 903 DIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNL 907

BLAST of Lsi07G000360 vs. TAIR 10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 179.9 bits (455), Expect = 1.2e-44
Identity = 210/950 (22.11%), Postives = 358/950 (37.68%), Query Frame = 0

Query: 8   KDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKD 67
           K F F   EE + ++W     F+   ++      ++    PP  TG  H GH +  T++D
Sbjct: 71  KTFDF-SSEEKIYKWWESQGYFKPNFDQGGS--PFVIPMPPPNVTGSLHMGHAMFVTLED 130

Query: 68  IVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKL---GIKRRDDVLKMGIDKYNEECRSI 127
           I+ RY  M G       G D  G+  +  +++ L   GIKR D    +G D++ +     
Sbjct: 131 IMVRYNRMNGRPTLWLPGTDHAGIATQLVVEKMLASEGIKRVD----LGRDEFTKRVWEW 190

Query: 128 VTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYST 187
             +Y G     I R G   D+  +  T+D +   +V   F +L++KGL+Y+G  ++ +S 
Sbjct: 191 KEKYGGTITNQIKRLGASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGSYMVNWSP 250

Query: 188 GCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCVNANF 247
             +T +S+ E    Y + P     + + V G P        TT P TL  ++AL V    
Sbjct: 251 NLQTAVSDLEV--EYSEEPGFLYHIKYRVAGSPDFLTIA--TTRPETLFGDVALAV---- 310

Query: 248 VYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTENAVE 307
                                                      +P+    S    + A+ 
Sbjct: 311 -------------------------------------------HPEDDRYSKYVGQTAI- 370

Query: 308 DSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPAFGED 367
                        +   ++VP              ++AD YV  D GTG++  +P    +
Sbjct: 371 -----------VPMTYGRHVP--------------IIADKYVDKDFGTGVLKISPGHDHN 430

Query: 368 DYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSG 427
           DY +  +      G  ++  ++ D         F G    +  + +   ++  G  VK  
Sbjct: 431 DYLLARK-----LGLPILNVMNKDATLNDVAGLFCGLDRFEVREKLWADLEEIGLAVKKE 490

Query: 428 SFTHSYPFCWRSDTPLIYRAVPSWQVHLFSILKSVICFCDK----------EKRFHNWLE 487
             T   P   R    +       W VH+  + +  +   +           EK +++WL 
Sbjct: 491 PHTLRVPRSQRGGEVIEPLVSKQWFVHMDPLAEKALLAVENKELTIIPERFEKIYNHWLT 550

Query: 488 NARDWAVSRSRFWGTPLPVW--VSEDGEE--ILVMDSIEKLEQLSGVKVFDLHRHKIDHI 547
           N +DW +SR  +WG  +PVW  V +D EE  I+   + E LE     K  + +   ++  
Sbjct: 551 NIKDWCISRQLWWGHRIPVWYVVGKDCEEDYIVAKSAEEALE-----KALEKYGKDVEIY 610

Query: 548 TIPSRRGLEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 607
             P              DV D WF S   P++ + +P    + F   +P + +  G D  
Sbjct: 611 QDP--------------DVLDTWFSSSLWPFSTLGWPDVAAKDFNNFYPTNMLETGHDIL 670

Query: 608 RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR 667
             W   ++++     G   F ++  +GL+    G+KMSK L N   P++ I D+G DALR
Sbjct: 671 FFWVARMVMMGIEFTGTVPFSHVYLHGLIRDSQGRKMSKSLGNVIDPLDTIKDFGTDALR 730

Query: 668 LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLE----IEGFAPFSP 727
             +        + L    E +          W NA +F++ +   L      E       
Sbjct: 731 FTIALG--TAGQDLNLSTERLTANKAFTNKLW-NAGKFVLHSLPSLSDTSAWENLLDLK- 790

Query: 728 VDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFL-DNLTNIYVRFN 787
           +D+     S  + + W  S    L+  V    +      V      F   +  + Y+  +
Sbjct: 791 LDKEETLLSLPLPECWAVSKLHILIDSVTASYEKLFFGDVGRETYDFFWSDFADWYIEAS 850

Query: 788 RKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYC 847
           + RL G  G      + + L +V     K++ PF PF TE L+Q L       +E++   
Sbjct: 851 KSRLYGSGGNSVSLASQAVLLYVFENILKLLHPFMPFVTEDLWQAL----PYRKEALIVS 892

Query: 848 SYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAG 907
            +PQ    R     ES+ R   +  L R IR           R    V P K     + G
Sbjct: 911 PWPQNSLPRN---VESIKRFENLQALTRAIRN---------ARAEYSVEPVKRISASVVG 892

Query: 908 --KLREYVLEELNIRSLIPCDDTLKYASLRAEPDFSVLGKRLGKAMGVVA 934
             ++ EY+ +E  + +L+   D        A P  + L   L  + G+ A
Sbjct: 971 SAEVIEYISKEKEVLALLSRLDLNNVHFSNAPPGDANLSVHLVASEGLEA 892