Lsi06G015420 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G015420
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionSpermidine synthase
Locationchr06: 25771974 .. 25779002 (-)
RNA-Seq ExpressionLsi06G015420
SyntenyLsi06G015420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AATAAACTTTTTTTTAATGAATAATTAATAGTAAATTAAATATATAGACTCAATCCTATTATCAAAGCATGTGGAAGATTTTATGAAGGGCCCACTTTTTTGCCACCAAGTTGCATTCTATATAAACCCCAAGACCCAACTCCATTACAAAGCCTAATTTTTTTTTTTTAAACAGAGTTTTAAAAGAAAAAAAAAATTTTAAAATTTTTTATTGTTGAATGGCCATGGATGCAAATGGGGTTCTTTCTTTTTCAGATGATTTGGTGTTTAAGCAGAAGAAAGATGAAGTTTTCAACAATGGCTGCTCTCATCAGCATGTTGCAGTTGAAAATCAAAAAGAAGAAGAAGAAGACGACTCCATTAATAGTGATGCTCTCAACCATCCAACCATTCCTGGTTGGTTCTCTGAGCACTGCCCACAATGGCCAGGTGGGTTTTTTCTTTTTTCTTATAAATTATTTTCAATTTTTTCAATTTACTTTTCCATTGTGTGTATTAATAATTTCATCCAAATTTTTTGAAAAGTTAAAGGACATGTTTGAAAGTGATTTTAAAATGGTTAAAATCACTTTTGTCATTTTTAAAATCACCGTGAAATATGTTTTTAATCTTTTAAAATTAATTTTGATGATTGAAAATTGCATCTAAAAGTGTAAAATTGAAAATTAAATTAATTTTGAGTGATTAAAGTGTGTTTTTATGATTTTAAAAATGACAAAAGTGATTTTAACGATTTTAAAATCACTCTCAAACATGTCTTAAGTGTTTGAGACAAATCTCTCTTTCACATCGTTTTTGTTATTAATACCAATCTAAACTAAGTGGATAAAGACGCAAAACCTAATTAGGAGAGTTTAGTTATTATAGTTAGTCGTTATTATAATATGTGGGTTATAATAGTTTGTAGGTTATAATAATTTAGGTTTAGTGGTTGACTTTTTTTTTAATTTTCATTTTGTGTAATAATAATTTGGTCCAAAATTTTGAAAAGTGTAAGGCCTATTTGGTTGAAAATAAAAATTTTGAAAACAATGAATTTGATGACAACATCGGTGTACTTCATATTTTTAGATATGAACATATTGAGGAATTGGATTCTAATTTAAAGAATTGTTTTAATGTATTTAATATTATATTTGTAATCTATAAAATTAGTTGTAATTACCAACTAAATTTTAAGTTGAATATAAAATGTAATTAATTCATTTATGAACATATTTTAATTATTTTTTTGGTATAATTATTATTTGTACTATTATATAATTTATAATAAATTACATAATAGATATTTTAATTTTTTTTAAAAAAAATTGAGTCTATGACATGAAATACCATATTTCTAAATGTTGTCATCTTTTTTTAAATATAATTTTGCATTTCAAGATTTAAAATTCAAAATTTTGATATACTGAACACATAAAAATGTTGTTTTCAAAATTTGAACTATTCTGATCACACTTAATTTGAAAATAGTTTTCAAAAACATAATCCAAATTGTCTGCAAACATATATTACTAAACTTAATGAATCTTAAAACATAAAATAGAATTTCGATTGTCAACAAAACAGATTTTTGATTATGTAAGTCAATATCTCTCTTCCGATTTGTTTTTGTTATTTCTATCAATTCAAATATGACTATATAGTAAAGATACAACCTATATATTACTTTCCTTAAAGTTGAAGAAGACTCAAAGGCCTATCACGCATTTCGTACTAAAAGAACAAAATTCATCTTTGTGTTATTACTTTAGGGGCAAAAGTATATGAGTTGATTGTATGAGAAAGAAATGAGTAAATTTTTTAAATTAAAACAAAAACAAAAAATCAAAAACAATAGATTAGTACTATTTGATGGTACCTACTAAATAATACTTTTTTAATATAACGAGGATGGGAGCTGGAACATCTTACTTAGCAGAGTGGATGCTAAATATCATTGAACTAAACTCGCTTTGACCCTATTAAAATTGTTTGTCACATGTACCTTTAGTTAGGATTTTCTTTTCTCTTTGGTAGAGCGTGTTTGAATTGATTTTCTAAGTGTTTATAAGTGGAAAAAAAATGTTTACAAAGTCAATCTAAATAGACTTTTAATGTCGGAAATCTTATTGTTAGATAGCATGCATTGGCCATTGTAATTATAAAACACAAAGCAAAATCCACTTTGAAAAACATGTTGGTTTAAGTCAGTGTGAAAAGGTTGTCTCTAGCTTCTAAAACATCATCCTTAATTATTAAACATTCTGTTTCAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACAAACATTTTGATGAGATCTATGATAAAAGATGAACTAATTAAAGATGGAATCAAATTTGTACAGGTCAAGCACACTTCTTAAAGGTGGACAAAGTTTTGTTTCAAGGAAAGTCCGATTACCAATCCATGTTGGTGTTTCAGGTATTATATACATATATACAAATATTCGAAACTTGAATATCCAAATATTTGACTTTTAAAAACAACGAGAAAAATACTTTTTCTCAGTCCCTTACTTTTGTTTTTTCTTTGAGTAATAGTTTTATTTGATCTCAAAGTTTCAAAATATAACATTTCTACTCCTAAGTTTTGAGTTAATTTCATTTTGTCTCCTAAATTTCATAATGTTACGTTTTTATTTCTTTTCTTTTTGACAATATGTGCGGTGGGAGAATTGGACTTTCCACCTCAAGGTCCGATAGTACAAATATTAGGTTAGTTTTTTTTTTTTTTTGTTGATAATTTGTGGGATGTGAGAGATTTTAATATCTAACTTCAAAGTCTATAGCATTCTTGTTGGTAGACAACGACCCTCTACAATTGTATGATATTGTCCATTTGGGTATAACTCTCGTGACTTTGTTTTTTGTTTCATTCAAAAAGTCTCATACTATTGGAAATAGTTGTCTTCTCTTATATACCATAGATTTTCCTCTTATCTAATTAATGTAGGATTTTGGGAGAGTTTTTCTAGTACTACGGGAATATCATACTCCATATATTCACATTATAAATTTGTTTCCATAAATATAAGTGGACATCACATTAATGTCACTGGGTCTCATCATATTATTAAAAGCAAGTTGACGTGACACACAATAAATTGAATGAGTACTCCCGTGGTATAAGAAATTTTTTAGGACTTTGGTCACATAATGATTATAAATTTGACACTTTTTTTTAAGGAAGAAAGTCAGATGAATAATTAATGTGAAAAACTAGTAACTTTTGAAAGAACTGATTTCAAAATTACCCAACAAGAAAGGGAGGCAAAAGGGAAAGGGAAAAAGGGAAAGTAATAATTAATTAAGCTCATGCTTAATCCTAATTAATTAGCTAGTTTATGAGTTTAGATTTGTTTATAATTATAATTAAAATGCAGTCATCAGCATATGGAAAGGTGTTTGTGTTGGATGGAGCACTCCAACTCACTGAGAAGGATGAATGTGCTTACCAAGAAATGATTACCCACCTTCCTCTTTGCTCTATTCCAAACCCCAAAAAGGTCATTTCCCTCTCTTACTTTTTCTCTCTCATCACTGTTTTTTTTAAGTTAAACTTTCTATTTTATGTCAAATAGATGTGGACAAATTTAAAATATTTAATAATTAATTGATCTATCATATATATATATATAAATTTAAATTTTATGTTGAATTAAACTATAAGACCTCGATTGGTAACTGTTTGGGTCTTGTTTGGTAACCAATTTCATTTTGGGTTTTTTGTTTTTGAAAATTAAGTCTATTTTCTCACATTTTCTTACAATGGTTTGCAACTTTGTTGAGTATCAGAGTTGAATTCTTAGTCAAATTCCAAAAACAAAAACAAATTTTTTTAGTTTTCAAATTTTGGATTGGTTTTTGAAAACATGGGTAAAAAATAGACGAGAAAACAAGTTATTTAGAGGTGAGATGGGTGTTTGTAGGCTTAATTTTCAAAAACTAAAAATCAAATGGTTATCAAATGAGGCCTTAGTTTTTTGTTTTTTAAAATTAAATCTATACACACTACTTCCATGTATTGGTTTATATGAGTTGTTGTTTACATTTTAGGAGTGCTTTCAAAATCAAAAACCAAACTAAAAAAAAAAAATAGTTTTGAAGAGCTTATTTTTGTTTTTGAAATTTTGGGTAAGAATTCATATATTTACATCCAAAATATGAAAACTATGATTAAAAAAAATTGTTTGAAATAAGCACGAATTTCAAAAACCTAAATAGTTATAAAACGAGATCTAAAGTTTCAATTTTGTGTCTAATAAATTTAATTTGTGAATTTTTAAAAATTCAAAAATTAAAAGACTAAATTTATAATTTTGAATATTAAAAGACTAAATTAACACGAACATCAAAATTTAGAGAATAAATTTATAATTTAGTTTTTTTTAAAAAAAAATAAAAAATTGTGACCTTATATTATTTATGACAATGTGGAAACATGCCATAAAATCATGAGGTGCTATACTAAAGCCTTTTAGCATATATATTATATCAAACTTTTAAGTTAGGATTAAGTTTAATTAATTTCCATTTGAAATCATAATTACTAGGCCACTTTGTATTTGTATTTTTTATTTTTTATTTATTTTTCATCAGGTTTTGCTGATTGGGGGAGGAGATGGTGGCATCCTAAGAGAAATTTCTAGGCATTCTTCCATTGAACAAATTGATATTTGTGAAATAGACACCATGCTCATTGACGTCAGTTCCTCTCTCTTTTTTTAAGCCCTAATTTTATTTTATTATTATTAATTACAATTTTAATTTTTTTACTTTTTGTAGTTTGTAGCTACCTAAACTTTTTATTTCGAGTTTAATAAGTCTTTGAAAATTCTAAATTCTAAAAAATTAATTGATCTATTTGATATAAATTTAAAATTTATGTCTACTCTAAACTTTTAAATTCGTGTCTAATAAATTTGTAAATTTAAAAAAAAAAATCGAAAGTTGAAATATTAAATTTGTAATCTTAATATTTCATCAACTAAATTTATAATTTATTATTATTGCCTCTTCTCTATTTCTTTAACATACTTTAAGACAAAATCTGAAAAAAAAATACCAAATATTTATTCCAAAACAAACTTTTGAAAGAGATTTATCCCAAGAATAATGTATTAAAAAAGCTAAAGTGAGACATGACCATCCTTCTTGGTCGAAGATTCGATCCTCCACACCACAGTTGTCGTACAAAAAAAATGTAAAAAAAAAAAAAAAAAAAGTTACATTCTAACTCATCAGGTTAAGTAGTTAGATTTAAGGGAAATTTTTAAAAATAAAAAAATAAGAAAACTATTTACATAAAATAGCAAAACTTTTAGATAGTTGTGAAAAATGTTGAAGTCTACCAGGGTCTGTTAGTGATAGAAATGATAGAAGTCTATCACTGATAGGTGCTGATAGAAATCTATCAATATCTATCAATATTTTTATTTTTATTTTTTTTTTGTTATTTTTTTGTAAATAGTTTGACATTTTTTCTATCGGTGAAAATTTCACTAGATTTAAATTAATTGAAATTCTAACATCATATCATAGTATTAAATTTTTTTAAATTTTTTTTTTTTTTTGAAGGTGTACAAGAAGTATTTTCCAGAAATTGCAGTTGGGTATAAAGATTCTCGAGTGAATCTTCACATAATAGATGGTTTGATTCAATTAATTATACTATTTTTCTAATTATTATTATTTTTAATATTATAATTTTTTTAATTATGTGTTTTTGCGTTTGAAGGAACTGCGTTTCTGAGTTCCGTTCCAGCAGGAACCTACGATGCAATCATAGTCGACGCTTTCGATCCAATAAGTACAATAATTAACAATATCAAATATATATATATAACTATAAATTAATTATAATTTATTTATTCTTATGTCTTATTTAAAAGCTGTTTAGAAAATATGGTTAGAAAAACTATATGTGGAGCAATTAGACATAAATTGATACCGACTCTCATTTTCTAAGAATGTGTTTGGAATACATTTTTAAGCGTTTAATTTAAAAATAAGTCATTTTAGAAAAGAAAAAAAAATTAGAATGTTTGATAACCACTTTTGAAGTGTATTTTAAATCATTGTTATCAAAAGAATTTAAATAAAAATGAATTTTTTAAAAAAATATTTTTTTTTCTCAAGTCAATCCAAGCGAGCCTTAAGTTTCAAAAAGTTGGATTTGTAGTATTAGATCTTGAGTTTTGAGTTTTATTTTCGATTGGTACTAGATTTCAAAATTTACATTTTTACTCTTGATTTTCAATAAATACACATTTTTCATCTTTATAATGAAAAGAATTATAGTTAATTTAGCTTTTATTAATTTTTCAAAATTAAGTGAATCTTTTTTTCACGTTTTAACTATTTTATTAATTAATTGAAAGTAAACATCAAAGACCAAAAGTGAGTTTAACTCAATGGTGGTATGTACTCTTTTTTTTTTTTAAGTCAGAGGGTATCTTCCATTCCTGTAATTATTATACTAAAAAATAAATGGCAAAAATGTAACCTTCTAAAAAATATAGAGAGACCAAAAGATTTTTTTTCCAAAAATAATAGTTTTTTTTTTTTTTTTTTTTTTTGATGTGGTTAGAGCCAGATGATGAGGTTGTGAGAAGTAGCTTGTTTGAGAAGGTGGCAAAAGCACTTAGGGATGGAGGAGTGCTTTGTATTCAAGCAGAAAGCCTTTGGTTTCATTCTCTTGACATTGAAATTTTGATCTCAAAATGCCAACAAGTATTCAAAGGCTCAGTTCAATATGCTTGGACCATTGTTCCTACTTATCCTAGGTATAAAAATCTCCATCATCTATATATAATCTATCAACTAATTAAATTTTTTTAAAAAAATAATAATAAGATCTAATGAATATATACATTGTGTTTTGTCAGTGGGGTAATTGGTTTCTTGTTGTGTTCCACTCAAGGACCTAAAGTTGACTTCAAAAATCCGATCAATCCCATTGACCCAAATGAGAATTTTGGAGTTGCAACCAAACCGTTGAAGTTTTACAATTCAGAGGCA

mRNA sequence

AATAAACTTTTTTTTAATGAATAATTAATAGTAAATTAAATATATAGACTCAATCCTATTATCAAAGCATGTGGAAGATTTTATGAAGGGCCCACTTTTTTGCCACCAAGTTGCATTCTATATAAACCCCAAGACCCAACTCCATTACAAAGCCTAATTTTTTTTTTTTAAACAGAGTTTTAAAAGAAAAAAAAAATTTTAAAATTTTTTATTGTTGAATGGCCATGGATGCAAATGGGGTTCTTTCTTTTTCAGATGATTTGGTGTTTAAGCAGAAGAAAGATGAAGTTTTCAACAATGGCTGCTCTCATCAGCATGTTGCAGTTGAAAATCAAAAAGAAGAAGAAGAAGACGACTCCATTAATAGTGATGCTCTCAACCATCCAACCATTCCTGGTTGGTTCTCTGAGCACTGCCCACAATGGCCAGGTCAAGCACACTTCTTAAAGGTGGACAAAGTTTTGTTTCAAGGAAAGTCCGATTACCAATCCATGTTGGTGTTTCAGTCATCAGCATATGGAAAGGTTTTGCTGATTGGGGGAGGAGATGGTGGCATCCTAAGAGAAATTTCTAGGCATTCTTCCATTGAACAAATTGATATTTGTGAAATAGACACCATGCTCATTGACGTGTACAAGAAGTATTTTCCAGAAATTGCAGTTGGGTATAAAGATTCTCGAGTGAATCTTCACATAATAGATGGAACTGCGTTTCTGAGTTCCGTTCCAGCAGGAACCTACGATGCAATCATAGTCGACGCTTTCGATCCAATAAAGCCAGATGATGAGGTTGTGAGAAGTAGCTTGTTTGAGAAGGTGGCAAAAGCACTTAGGGATGGAGGAGTGCTTTGTATTCAAGCAGAAAGCCTTTGGTTTCATTCTCTTGACATTGAAATTTTGATCTCAAAATGCCAACAAGTATTCAAAGGCTCAGTTCAATATGCTTGGACCATTGTTCCTACTTATCCTAGTGGGGTAATTGGTTTCTTGTTGTGTTCCACTCAAGGACCTAAAGTTGACTTCAAAAATCCGATCAATCCCATTGACCCAAATGAGAATTTTGGAGTTGCAACCAAACCGTTGAAGTTTTACAATTCAGAGGCA

Coding sequence (CDS)

ATGGCCATGGATGCAAATGGGGTTCTTTCTTTTTCAGATGATTTGGTGTTTAAGCAGAAGAAAGATGAAGTTTTCAACAATGGCTGCTCTCATCAGCATGTTGCAGTTGAAAATCAAAAAGAAGAAGAAGAAGACGACTCCATTAATAGTGATGCTCTCAACCATCCAACCATTCCTGGTTGGTTCTCTGAGCACTGCCCACAATGGCCAGGTCAAGCACACTTCTTAAAGGTGGACAAAGTTTTGTTTCAAGGAAAGTCCGATTACCAATCCATGTTGGTGTTTCAGTCATCAGCATATGGAAAGGTTTTGCTGATTGGGGGAGGAGATGGTGGCATCCTAAGAGAAATTTCTAGGCATTCTTCCATTGAACAAATTGATATTTGTGAAATAGACACCATGCTCATTGACGTGTACAAGAAGTATTTTCCAGAAATTGCAGTTGGGTATAAAGATTCTCGAGTGAATCTTCACATAATAGATGGAACTGCGTTTCTGAGTTCCGTTCCAGCAGGAACCTACGATGCAATCATAGTCGACGCTTTCGATCCAATAAAGCCAGATGATGAGGTTGTGAGAAGTAGCTTGTTTGAGAAGGTGGCAAAAGCACTTAGGGATGGAGGAGTGCTTTGTATTCAAGCAGAAAGCCTTTGGTTTCATTCTCTTGACATTGAAATTTTGATCTCAAAATGCCAACAAGTATTCAAAGGCTCAGTTCAATATGCTTGGACCATTGTTCCTACTTATCCTAGTGGGGTAATTGGTTTCTTGTTGTGTTCCACTCAAGGACCTAAAGTTGACTTCAAAAATCCGATCAATCCCATTGACCCAAATGAGAATTTTGGAGTTGCAACCAAACCGTTGAAGTTTTACAATTCAGAGGCA

Protein sequence

MAMDANGVLSFSDDLVFKQKKDEVFNNGCSHQHVAVENQKEEEEDDSINSDALNHPTIPGWFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAYGKVLLIGGGDGGILREISRHSSIEQIDICEIDTMLIDVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIVDAFDPIKPDDEVVRSSLFEKVAKALRDGGVLCIQAESLWFHSLDIEILISKCQQVFKGSVQYAWTIVPTYPSGVIGFLLCSTQGPKVDFKNPINPIDPNENFGVATKPLKFYNSEA
Homology
BLAST of Lsi06G015420 vs. ExPASy Swiss-Prot
Match: Q9ZS45 (Spermidine synthase OS=Solanum lycopersicum OX=4081 GN=SPDSYN PE=2 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 2.5e-92
Identity = 170/304 (55.92%), Postives = 210/304 (69.08%), Query Frame = 0

Query: 26  NNGCSHQHVAVENQKEEEEDDSINSDALNHPTIPGWFSEHCPQWPGQAHFLKVDKVLFQG 85
           NNGC          ++EE    I+S       +PGWFSE  P WPG+AH LKV+K+LFQG
Sbjct: 35  NNGC----------EKEESSPYISS------VLPGWFSEISPLWPGEAHSLKVEKILFQG 94

Query: 86  KSDYQSMLVFQSSAYG-----------------------------------KVLLIGGGD 145
           KSDYQ++LVFQSS YG                                   KVL+IGGGD
Sbjct: 95  KSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGD 154

Query: 146 GGILREISRHSSIEQIDICEIDTMLIDVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVP 205
           GG+LRE+SRHSS+EQIDICEID M+++V K++FP++AVGY+D RVNLHI DG AFL +VP
Sbjct: 155 GGVLREVSRHSSVEQIDICEIDKMVVEVAKQFFPDVAVGYEDPRVNLHIGDGVAFLKNVP 214

Query: 206 AGTYDAIIVDAFDPIKPDDEVVRSSLFEKVAKALRDGGVLCIQAESLWFHSLDIEILISK 265
           AGTYDA+IVD+ DPI P  E+     FE +AKALR GGV+  QAES+W H   IE +++ 
Sbjct: 215 AGTYDAVIVDSSDPIGPAQELFEKPFFESIAKALRPGGVVSTQAESIWLHMHIIEEIVAN 274

Query: 266 CQQVFKGSVQYAWTIVPTYPSGVIGFLLCSTQGPKVDFKNPINPIDPNENFGVATKPLKF 295
           C+Q+FKGSV YAWT VPTYPSG+IGF+LCST+GP VDFKNPINPID +E+   + +PLKF
Sbjct: 275 CRQIFKGSVNYAWTTVPTYPSGMIGFMLCSTEGPAVDFKNPINPID-DESPAKSIEPLKF 321

BLAST of Lsi06G015420 vs. ExPASy Swiss-Prot
Match: O48659 (Spermidine synthase 2 OS=Hyoscyamus niger OX=4079 PE=2 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 2.5e-92
Identity = 163/286 (56.99%), Postives = 198/286 (69.23%), Query Frame = 0

Query: 44  EDDSINSDALNHPTIPGWFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAYG-- 103
           E+   N  A     +PGWFSE  P WPG+AH LKV+K+LFQGKSDYQ+++VFQSS YG  
Sbjct: 2   EEQGNNESAYISSILPGWFSEISPLWPGEAHSLKVEKILFQGKSDYQNVVVFQSSTYGKV 61

Query: 104 ---------------------------------KVLLIGGGDGGILREISRHSSIEQIDI 163
                                            KVL+IGGGDGG+LRE+SRHSS+EQIDI
Sbjct: 62  LVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVSRHSSVEQIDI 121

Query: 164 CEIDTMLIDVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIVDAFDPIKPD 223
           CEID M+IDV K++FP +A+GY+D RV LH+ DG AFL +VP GTYDA+IVD+ DPI P 
Sbjct: 122 CEIDKMVIDVSKQFFPNVAIGYEDPRVKLHVGDGVAFLKNVPEGTYDAVIVDSSDPIGPA 181

Query: 224 DEVVRSSLFEKVAKALRDGGVLCIQAESLWFHSLDIEILISKCQQVFKGSVQYAWTIVPT 283
            E+     FE VA+AL  GGV+C QAES+W H   IE ++S C+Q+FKGSV YAWT VPT
Sbjct: 182 QELFEKPFFESVARALCPGGVVCTQAESIWLHMHIIEDIVSNCRQIFKGSVNYAWTTVPT 241

Query: 284 YPSGVIGFLLCSTQGPKVDFKNPINPIDPNENFGVATKPLKFYNSE 295
           YPSGVIGF+LCST+GP VDFKNPINPID +++      PLKFYNSE
Sbjct: 242 YPSGVIGFMLCSTEGPAVDFKNPINPIDADDSHTKTRGPLKFYNSE 287

BLAST of Lsi06G015420 vs. ExPASy Swiss-Prot
Match: O82147 (Spermidine synthase OS=Coffea arabica OX=13443 PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.2e-92
Identity = 168/294 (57.14%), Postives = 204/294 (69.39%), Query Frame = 0

Query: 38  NQKEE--EEDDSINSDALNHPTIPGWFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVF 97
           N KEE  +  D ++S       IPGWFSE  P WPG+AH LKV+K+LFQGKSDYQ+++VF
Sbjct: 8   NSKEEAAQLPDGVSS------VIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQNVMVF 67

Query: 98  QSSAYG-----------------------------------KVLLIGGGDGGILREISRH 157
           QSS YG                                   KVL+IGGGDGG+LRE++RH
Sbjct: 68  QSSTYGKVLVLDGVIQLTERDECAYQEMIAHLPLCSIPSPKKVLVIGGGDGGVLREVARH 127

Query: 158 SSIEQIDICEIDTMLIDVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIVD 217
            S+EQIDICEID M++DV K++FP++AVG++D RV LHI DG AFL +VP GTYDAIIVD
Sbjct: 128 LSVEQIDICEIDKMVVDVSKQFFPDVAVGFEDPRVVLHIGDGVAFLKAVPEGTYDAIIVD 187

Query: 218 AFDPIKPDDEVVRSSLFEKVAKALRDGGVLCIQAESLWFHSLDIEILISKCQQVFKGSVQ 277
           + DPI P  E+     FE VAKALR GGV+C QAES+W H   IE +++ C+Q+FKGSV 
Sbjct: 188 SSDPIGPAQELFEKPFFESVAKALRPGGVVCTQAESIWLHMHIIEDIVANCRQIFKGSVN 247

Query: 278 YAWTIVPTYPSGVIGFLLCSTQGPKVDFKNPINPIDPNENFGVATKPLKFYNSE 295
           YAWT VPTYPSGVIGF+LCST+GP VDFK+PINPID N+      KPLKFYNSE
Sbjct: 248 YAWTTVPTYPSGVIGFMLCSTEGPPVDFKHPINPIDANDGRSKTMKPLKFYNSE 295

BLAST of Lsi06G015420 vs. ExPASy Swiss-Prot
Match: O48658 (Spermidine synthase 1 OS=Hyoscyamus niger OX=4079 PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 2.7e-91
Identity = 168/304 (55.26%), Postives = 209/304 (68.75%), Query Frame = 0

Query: 26  NNGCSHQHVAVENQKEEEEDDSINSDALNHPTIPGWFSEHCPQWPGQAHFLKVDKVLFQG 85
           +NGCS+      N+KE     S+         +PGWFSE  P WPG+AH LKV+K+LFQG
Sbjct: 7   SNGCSN------NEKESPYISSV---------LPGWFSEISPLWPGEAHSLKVEKILFQG 66

Query: 86  KSDYQSMLVFQSSAYG-----------------------------------KVLLIGGGD 145
           KSDYQ+++VFQSS YG                                   KVL+IGGGD
Sbjct: 67  KSDYQNVMVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGD 126

Query: 146 GGILREISRHSSIEQIDICEIDTMLIDVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVP 205
           GG+LRE+SRHSS+EQIDICEID M+++V K++FP++AVGY+D RVNLHI DG AFL +VP
Sbjct: 127 GGVLREVSRHSSVEQIDICEIDKMVVEVAKEFFPDVAVGYEDPRVNLHIGDGVAFLKNVP 186

Query: 206 AGTYDAIIVDAFDPIKPDDEVVRSSLFEKVAKALRDGGVLCIQAESLWFHSLDIEILISK 265
           AGTYDA+IVD+ DPI P  E+     FE +A+ALR GGV+  QAES+W H   IE +++ 
Sbjct: 187 AGTYDAVIVDSSDPIGPAQELFEKPFFESIARALRPGGVVSTQAESIWLHMHIIEEIVAN 246

Query: 266 CQQVFKGSVQYAWTIVPTYPSGVIGFLLCSTQGPKVDFKNPINPIDPNENFGVATKPLKF 295
           C+Q+FKGSV YAWT VPTYPSG+IGF+LCST+GP VDFKNPINPID +E+      PLKF
Sbjct: 247 CRQIFKGSVNYAWTTVPTYPSGMIGFMLCSTEGPAVDFKNPINPID-DESGPKTIAPLKF 294

BLAST of Lsi06G015420 vs. ExPASy Swiss-Prot
Match: Q96557 (Spermidine synthase 2 OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 2.7e-91
Identity = 166/304 (54.61%), Postives = 209/304 (68.75%), Query Frame = 0

Query: 26  NNGCSHQHVAVENQKEEEEDDSINSDALNHPTIPGWFSEHCPQWPGQAHFLKVDKVLFQG 85
           NNGC++          ++E   I+S       +PGWFSE  P WPG+AH LKV+K+LFQG
Sbjct: 10  NNGCTN----------DKESPCISS------VLPGWFSEISPLWPGEAHSLKVEKILFQG 69

Query: 86  KSDYQSMLVFQSSAYG-----------------------------------KVLLIGGGD 145
           KSDYQ ++VFQS+ YG                                   KVL+IGGGD
Sbjct: 70  KSDYQDVMVFQSTTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGD 129

Query: 146 GGILREISRHSSIEQIDICEIDTMLIDVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVP 205
           GG+LRE+SRHSS+EQIDICEID M+++V K++FP++AVGY+D RVNLHI DG AFL +VP
Sbjct: 130 GGVLREVSRHSSVEQIDICEIDKMVVEVAKQFFPDVAVGYEDPRVNLHIGDGVAFLKNVP 189

Query: 206 AGTYDAIIVDAFDPIKPDDEVVRSSLFEKVAKALRDGGVLCIQAESLWFHSLDIEILISK 265
           AGTYDA+IVD+ DPI P  E+     FE +A+ALR GGV+  QAES+W H   IE +++ 
Sbjct: 190 AGTYDAVIVDSSDPIGPAQELFEKPFFETIARALRPGGVVSTQAESIWLHMHIIEEIVAN 249

Query: 266 CQQVFKGSVQYAWTIVPTYPSGVIGFLLCSTQGPKVDFKNPINPIDPNENFGVATKPLKF 295
           C+++FKGSV YAWT VPTYPSG+IGF+LCST+GP VDFKNPINPID +E+ G    PLKF
Sbjct: 250 CREIFKGSVNYAWTTVPTYPSGMIGFMLCSTEGPAVDFKNPINPID-DESHGQTIGPLKF 296

BLAST of Lsi06G015420 vs. ExPASy TrEMBL
Match: A0A6J1JLE9 (spermidine synthase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485649 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.4e-138
Identity = 251/329 (76.29%), Postives = 270/329 (82.07%), Query Frame = 0

Query: 1   MAMDANGVLSFSDDLVFKQKKDEVFNNGCSHQHVAVENQKEEEEDDSINSDALNHPTIPG 60
           MAM+ANGV+SF+D L+FKQ+K+E FNNG SH H+AV    E+ EDDSIN   LNHP+IPG
Sbjct: 1   MAMEANGVVSFADKLLFKQQKEEAFNNGGSHLHLAV---AEKVEDDSINGVPLNHPSIPG 60

Query: 61  WFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAYG------------------- 120
           WFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQS+AYG                   
Sbjct: 61  WFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSAAYGKVFVLDGALQLTEKDECAY 120

Query: 121 ----------------KVLLIGGGDGGILREISRHSSIEQIDICEIDTMLIDVYKKYFPE 180
                           KVL+IGGGDGGILREISRHSSI+QIDICEIDTMLI+VYKK+FPE
Sbjct: 121 QEMITHLPLCSIPNPKKVLVIGGGDGGILREISRHSSIDQIDICEIDTMLIEVYKKFFPE 180

Query: 181 IAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIVDAFDPIKPDDEVVRSSLFEKVAKALR 240
           IAVGYKDSRVN+HIIDG AFL+SV  GTYDAIIVDAFDPIKPDDEVVRS LFE V KALR
Sbjct: 181 IAVGYKDSRVNVHIIDGIAFLNSVTPGTYDAIIVDAFDPIKPDDEVVRSRLFEMVGKALR 240

Query: 241 DGGVLCIQAESLWFHSLDIEILISKCQQVFKGSVQYAWTIVPTYPSGVIGFLLCSTQGPK 295
            GGVLCIQAESLWFH LDI+ LISKC QVFKGSVQYAWTIVP YPSGVIGFLLCST+GPK
Sbjct: 241 AGGVLCIQAESLWFHCLDIQNLISKCHQVFKGSVQYAWTIVPAYPSGVIGFLLCSTRGPK 300

BLAST of Lsi06G015420 vs. ExPASy TrEMBL
Match: A0A6J1E1D2 (spermidine synthase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111429923 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.4e-138
Identity = 252/329 (76.60%), Postives = 270/329 (82.07%), Query Frame = 0

Query: 1   MAMDANGVLSFSDDLVFKQKKDEVFNNGCSHQHVAVENQKEEEEDDSINSDALNHPTIPG 60
           MAM+ANGV+SF+D L+FKQ+K+E FNNG SH H+AV    E+ EDDSIN   L+HP+IPG
Sbjct: 1   MAMEANGVVSFADKLLFKQQKEEAFNNGGSHLHLAV---AEKVEDDSINGVPLDHPSIPG 60

Query: 61  WFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAYG------------------- 120
           WFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQS+AYG                   
Sbjct: 61  WFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSAAYGKVFVLDGALQLTEKDECAY 120

Query: 121 ----------------KVLLIGGGDGGILREISRHSSIEQIDICEIDTMLIDVYKKYFPE 180
                           KVL+IGGGDGGILREISRHSSI+QIDICEIDTMLIDVYKK+FPE
Sbjct: 121 QEMITHLPLCSIPNPKKVLVIGGGDGGILREISRHSSIDQIDICEIDTMLIDVYKKFFPE 180

Query: 181 IAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIVDAFDPIKPDDEVVRSSLFEKVAKALR 240
           IAVGYKDSRVN+HIIDG AFL+SV  GTYDAIIVDAFDPIKPDDEVVRS LFE VA+ALR
Sbjct: 181 IAVGYKDSRVNVHIIDGIAFLNSVAPGTYDAIIVDAFDPIKPDDEVVRSRLFEMVARALR 240

Query: 241 DGGVLCIQAESLWFHSLDIEILISKCQQVFKGSVQYAWTIVPTYPSGVIGFLLCSTQGPK 295
            GGVLCIQAESLWFH LDI+ LISKC QVFKGSVQYAWTIVP YPSGVIGFLLCSTQGPK
Sbjct: 241 AGGVLCIQAESLWFHCLDIQNLISKCHQVFKGSVQYAWTIVPAYPSGVIGFLLCSTQGPK 300

BLAST of Lsi06G015420 vs. ExPASy TrEMBL
Match: A0A0A0LP68 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G005540 PE=3 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.6e-137
Identity = 259/340 (76.18%), Postives = 274/340 (80.59%), Query Frame = 0

Query: 3   MDANGVLSFSDDLVFKQKKDEVF--NNGCSHQHVAV-------ENQKEEEEDDSI---NS 62
           MDANGVLSFSDDLVFKQKKDEV   NNGCS  HV V       ++++EEEED+SI     
Sbjct: 1   MDANGVLSFSDDLVFKQKKDEVLINNNGCS-DHVQVSEVMEDKKDKEEEEEDNSIIINGD 60

Query: 63  DALNHPTIPGWFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAYG--------- 122
           D LN+PTIPGWFSEHCPQWPGQAHFLKVDKVLFQG S YQSMLVFQ+SAYG         
Sbjct: 61  DPLNYPTIPGWFSEHCPQWPGQAHFLKVDKVLFQGNSAYQSMLVFQTSAYGKVFVLDGAL 120

Query: 123 --------------------------KVLLIGGGDGGILREISRHSSIEQIDICEIDTML 182
                                     KVL+IGGGDGGILREISRHSSIE IDICEIDTML
Sbjct: 121 QLTEKDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGILREISRHSSIEHIDICEIDTML 180

Query: 183 IDVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIVDAFDPIKPDDEVVRSS 242
           IDVYKKYFPEIAVGYKD RVNLHIIDG  FLSSVP G+YDAIIVDAFDPIKPDDEV+RS 
Sbjct: 181 IDVYKKYFPEIAVGYKDYRVNLHIIDGNVFLSSVPPGSYDAIIVDAFDPIKPDDEVMRSK 240

Query: 243 LFEKVAKALRDGGVLCIQAESLWFHSLDIEILISKCQQVFKGSVQYAWTIVPTYPSGVIG 295
           LFEKVAKAL++GGVLCIQAESLWFHSLDI+ILISKC+QVFKGSVQYAWTIVP YPSGVIG
Sbjct: 241 LFEKVAKALKEGGVLCIQAESLWFHSLDIQILISKCKQVFKGSVQYAWTIVPAYPSGVIG 300

BLAST of Lsi06G015420 vs. ExPASy TrEMBL
Match: A0A1S3BVF4 (spermidine synthase 2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493919 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.4e-137
Identity = 259/345 (75.07%), Postives = 274/345 (79.42%), Query Frame = 0

Query: 3   MDANGVLSFSDDLVFKQKKDEVFN----NGCSHQHVA----------VENQKEEEEDDSI 62
           MDANGVLSFSDDLVFK+KKDEV N    NGCSH  V            E ++EEEED+SI
Sbjct: 1   MDANGVLSFSDDLVFKKKKDEVLNNNNDNGCSHLQVLEAEHQKDEEDEEEEEEEEEDNSI 60

Query: 63  ---NSDALNHPTIPGWFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAYG---- 122
                D L+HPTIPGWF EHCPQWPGQAHFLKVDKVLFQGKS YQ+MLVFQSSAYG    
Sbjct: 61  IINGDDPLDHPTIPGWFCEHCPQWPGQAHFLKVDKVLFQGKSAYQAMLVFQSSAYGKVFV 120

Query: 123 -------------------------------KVLLIGGGDGGILREISRHSSIEQIDICE 182
                                          KVL+IGGGDGGILREISRHSSIEQIDICE
Sbjct: 121 LDGALQLTEKDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGILREISRHSSIEQIDICE 180

Query: 183 IDTMLIDVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIVDAFDPIKPDDE 242
           IDTMLIDVYKKYFPEIAVGYKD RVNLHIIDG+AFLSSVP G+YDAIIVDAFDPIKPDDE
Sbjct: 181 IDTMLIDVYKKYFPEIAVGYKDYRVNLHIIDGSAFLSSVPPGSYDAIIVDAFDPIKPDDE 240

Query: 243 VVRSSLFEKVAKALRDGGVLCIQAESLWFHSLDIEILISKCQQVFKGSVQYAWTIVPTYP 295
           VVRS LFE VAKAL++GGVLCIQAESLWF SLDI+ILISKC+QVFKGSVQYAWTIVP YP
Sbjct: 241 VVRSKLFENVAKALKEGGVLCIQAESLWFPSLDIQILISKCKQVFKGSVQYAWTIVPAYP 300

BLAST of Lsi06G015420 vs. ExPASy TrEMBL
Match: A0A5A7UWC7 (Spermidine synthase 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001770 PE=3 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.1e-134
Identity = 257/346 (74.28%), Postives = 272/346 (78.61%), Query Frame = 0

Query: 3   MDANGVLSFSDDLVFKQKKDEVFN----NGCSHQHVA----------VENQKEEEEDDSI 62
           MDANGVLSFSDDLVFK+KKDEV N    NGCSH  V            E ++EEEED+SI
Sbjct: 1   MDANGVLSFSDDLVFKKKKDEVLNNNNDNGCSHLQVLEAEHQKDEEDEEEEEEEEEDNSI 60

Query: 63  ---NSDALNHPTIPGWFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAYG---- 122
                D L+HPTIPGWF EHCPQWPGQAHFLKVDKVLFQGKS YQ+ML   SSAYG    
Sbjct: 61  IINGDDPLDHPTIPGWFCEHCPQWPGQAHFLKVDKVLFQGKSAYQAML---SSAYGKVFV 120

Query: 123 -------------------------------KVLLIGGGDGGILREISRHSSIEQIDICE 182
                                          KVL+IGGGDGGILREISRHSSIEQIDICE
Sbjct: 121 LDGALQLTEKDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGILREISRHSSIEQIDICE 180

Query: 183 IDTMLIDVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIVDAFDPIKPDDE 242
           IDTMLIDVYKKYFPEIAVGYKD RVNLHIIDG+AFLSSVP G+YDAIIVDAFDPIKPDDE
Sbjct: 181 IDTMLIDVYKKYFPEIAVGYKDYRVNLHIIDGSAFLSSVPPGSYDAIIVDAFDPIKPDDE 240

Query: 243 VVRSSLFEKVAKALRDGGVLCIQAESLWFHSLDIEILISKCQQVFKGSVQYAWTIVPTYP 296
           VVRS LFE VAKAL++GGVLCIQAESLWF SLDI+ILISKC+QVFKGSVQYAWTIVP YP
Sbjct: 241 VVRSKLFENVAKALKEGGVLCIQAESLWFPSLDIQILISKCKQVFKGSVQYAWTIVPAYP 300

BLAST of Lsi06G015420 vs. NCBI nr
Match: XP_038879915.1 (spermidine synthase 2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 526.2 bits (1354), Expect = 1.9e-145
Identity = 276/373 (73.99%), Postives = 286/373 (76.68%), Query Frame = 0

Query: 1   MAMDANGVLSFSDDLVFKQKKDEVFNNGCSHQ-HV-AVENQKEEEEDDSI---------- 60
           MAMDANGVLSFSDDLVFKQKK+EVFNNGCSH  HV +VE+Q  +EEDDSI          
Sbjct: 1   MAMDANGVLSFSDDLVFKQKKEEVFNNGCSHNLHVISVEDQNLDEEDDSIKINGDGDALN 60

Query: 61  --------------------------------NSDALNHPTIPGWFSEHCPQWPGQAHFL 120
                                           ++DALNHPTIPGWFSEHCPQWPGQAHFL
Sbjct: 61  HPTIPGWFSEHCPQWPVEEKKDEEDDSIKINGDADALNHPTIPGWFSEHCPQWPGQAHFL 120

Query: 121 KVDKVLFQGKSDYQSMLVFQSSAYG----------------------------------- 180
           KVDKVLFQGKSDYQSMLVFQSSAYG                                   
Sbjct: 121 KVDKVLFQGKSDYQSMLVFQSSAYGKVFVLDGALQLTEKDECAYQEMITHLPLCSIPNPK 180

Query: 181 KVLLIGGGDGGILREISRHSSIEQIDICEIDTMLIDVYKKYFPEIAVGYKDSRVNLHIID 240
           KVLLIGGGDGGILREISRHSSIEQIDICEIDTMLIDVYKKYFPEIAVGYKD RVNLHIID
Sbjct: 181 KVLLIGGGDGGILREISRHSSIEQIDICEIDTMLIDVYKKYFPEIAVGYKDYRVNLHIID 240

Query: 241 GTAFLSSVPAGTYDAIIVDAFDPIKPDDEVVRSSLFEKVAKALRDGGVLCIQAESLWFHS 295
           G AFL+SVPAGTYDAIIVDAFDPIKPDDEVV++ LFEKVAKALRDGGVLCIQAESLWFHS
Sbjct: 241 GIAFLNSVPAGTYDAIIVDAFDPIKPDDEVVKNGLFEKVAKALRDGGVLCIQAESLWFHS 300

BLAST of Lsi06G015420 vs. NCBI nr
Match: XP_038879914.1 (spermidine synthase 2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 517.7 bits (1332), Expect = 6.6e-143
Identity = 274/398 (68.84%), Postives = 284/398 (71.36%), Query Frame = 0

Query: 1   MAMDANGVLSFSDDLVFKQKKDEVFNNGCSHQ---------------------------- 60
           MAMDANGVLSFSDDLVFKQKK+EVFNNGCSH                             
Sbjct: 1   MAMDANGVLSFSDDLVFKQKKEEVFNNGCSHNLHVISVEDQNLDEEDDSIKINGDGDALN 60

Query: 61  -------------------------------------HVAVENQKEEEEDDSI----NSD 120
                                                HV    +K++EEDDSI    ++D
Sbjct: 61  HPTIPGWFSEHCPQWPDDLVFKQKKKEVFNHGSSHNLHVIAVEEKKDEEDDSIKINGDAD 120

Query: 121 ALNHPTIPGWFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAYG---------- 180
           ALNHPTIPGWFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAYG          
Sbjct: 121 ALNHPTIPGWFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAYGKVFVLDGALQ 180

Query: 181 -------------------------KVLLIGGGDGGILREISRHSSIEQIDICEIDTMLI 240
                                    KVLLIGGGDGGILREISRHSSIEQIDICEIDTMLI
Sbjct: 181 LTEKDECAYQEMITHLPLCSIPNPKKVLLIGGGDGGILREISRHSSIEQIDICEIDTMLI 240

Query: 241 DVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIVDAFDPIKPDDEVVRSSL 295
           DVYKKYFPEIAVGYKD RVNLHIIDG AFL+SVPAGTYDAIIVDAFDPIKPDDEVV++ L
Sbjct: 241 DVYKKYFPEIAVGYKDYRVNLHIIDGIAFLNSVPAGTYDAIIVDAFDPIKPDDEVVKNGL 300

BLAST of Lsi06G015420 vs. NCBI nr
Match: XP_022988394.1 (spermidine synthase 1-like [Cucurbita maxima])

HSP 1 Score: 502.3 bits (1292), Expect = 2.9e-138
Identity = 251/329 (76.29%), Postives = 270/329 (82.07%), Query Frame = 0

Query: 1   MAMDANGVLSFSDDLVFKQKKDEVFNNGCSHQHVAVENQKEEEEDDSINSDALNHPTIPG 60
           MAM+ANGV+SF+D L+FKQ+K+E FNNG SH H+AV    E+ EDDSIN   LNHP+IPG
Sbjct: 1   MAMEANGVVSFADKLLFKQQKEEAFNNGGSHLHLAV---AEKVEDDSINGVPLNHPSIPG 60

Query: 61  WFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAYG------------------- 120
           WFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQS+AYG                   
Sbjct: 61  WFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSAAYGKVFVLDGALQLTEKDECAY 120

Query: 121 ----------------KVLLIGGGDGGILREISRHSSIEQIDICEIDTMLIDVYKKYFPE 180
                           KVL+IGGGDGGILREISRHSSI+QIDICEIDTMLI+VYKK+FPE
Sbjct: 121 QEMITHLPLCSIPNPKKVLVIGGGDGGILREISRHSSIDQIDICEIDTMLIEVYKKFFPE 180

Query: 181 IAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIVDAFDPIKPDDEVVRSSLFEKVAKALR 240
           IAVGYKDSRVN+HIIDG AFL+SV  GTYDAIIVDAFDPIKPDDEVVRS LFE V KALR
Sbjct: 181 IAVGYKDSRVNVHIIDGIAFLNSVTPGTYDAIIVDAFDPIKPDDEVVRSRLFEMVGKALR 240

Query: 241 DGGVLCIQAESLWFHSLDIEILISKCQQVFKGSVQYAWTIVPTYPSGVIGFLLCSTQGPK 295
            GGVLCIQAESLWFH LDI+ LISKC QVFKGSVQYAWTIVP YPSGVIGFLLCST+GPK
Sbjct: 241 AGGVLCIQAESLWFHCLDIQNLISKCHQVFKGSVQYAWTIVPAYPSGVIGFLLCSTRGPK 300

BLAST of Lsi06G015420 vs. NCBI nr
Match: XP_022921767.1 (spermidine synthase 1-like [Cucurbita moschata])

HSP 1 Score: 502.3 bits (1292), Expect = 2.9e-138
Identity = 252/329 (76.60%), Postives = 270/329 (82.07%), Query Frame = 0

Query: 1   MAMDANGVLSFSDDLVFKQKKDEVFNNGCSHQHVAVENQKEEEEDDSINSDALNHPTIPG 60
           MAM+ANGV+SF+D L+FKQ+K+E FNNG SH H+AV    E+ EDDSIN   L+HP+IPG
Sbjct: 1   MAMEANGVVSFADKLLFKQQKEEAFNNGGSHLHLAV---AEKVEDDSINGVPLDHPSIPG 60

Query: 61  WFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAYG------------------- 120
           WFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQS+AYG                   
Sbjct: 61  WFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSAAYGKVFVLDGALQLTEKDECAY 120

Query: 121 ----------------KVLLIGGGDGGILREISRHSSIEQIDICEIDTMLIDVYKKYFPE 180
                           KVL+IGGGDGGILREISRHSSI+QIDICEIDTMLIDVYKK+FPE
Sbjct: 121 QEMITHLPLCSIPNPKKVLVIGGGDGGILREISRHSSIDQIDICEIDTMLIDVYKKFFPE 180

Query: 181 IAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIVDAFDPIKPDDEVVRSSLFEKVAKALR 240
           IAVGYKDSRVN+HIIDG AFL+SV  GTYDAIIVDAFDPIKPDDEVVRS LFE VA+ALR
Sbjct: 181 IAVGYKDSRVNVHIIDGIAFLNSVAPGTYDAIIVDAFDPIKPDDEVVRSRLFEMVARALR 240

Query: 241 DGGVLCIQAESLWFHSLDIEILISKCQQVFKGSVQYAWTIVPTYPSGVIGFLLCSTQGPK 295
            GGVLCIQAESLWFH LDI+ LISKC QVFKGSVQYAWTIVP YPSGVIGFLLCSTQGPK
Sbjct: 241 AGGVLCIQAESLWFHCLDIQNLISKCHQVFKGSVQYAWTIVPAYPSGVIGFLLCSTQGPK 300

BLAST of Lsi06G015420 vs. NCBI nr
Match: XP_011657853.1 (spermidine synthase 2 isoform X1 [Cucumis sativus] >KAE8652405.1 hypothetical protein Csa_013137 [Cucumis sativus])

HSP 1 Score: 501.5 bits (1290), Expect = 4.9e-138
Identity = 261/342 (76.32%), Postives = 276/342 (80.70%), Query Frame = 0

Query: 1   MAMDANGVLSFSDDLVFKQKKDEVF--NNGCSHQHVAV-------ENQKEEEEDDSI--- 60
           MAMDANGVLSFSDDLVFKQKKDEV   NNGCS  HV V       ++++EEEED+SI   
Sbjct: 1   MAMDANGVLSFSDDLVFKQKKDEVLINNNGCS-DHVQVSEVMEDKKDKEEEEEDNSIIIN 60

Query: 61  NSDALNHPTIPGWFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAYG------- 120
             D LN+PTIPGWFSEHCPQWPGQAHFLKVDKVLFQG S YQSMLVFQ+SAYG       
Sbjct: 61  GDDPLNYPTIPGWFSEHCPQWPGQAHFLKVDKVLFQGNSAYQSMLVFQTSAYGKVFVLDG 120

Query: 121 ----------------------------KVLLIGGGDGGILREISRHSSIEQIDICEIDT 180
                                       KVL+IGGGDGGILREISRHSSIE IDICEIDT
Sbjct: 121 ALQLTEKDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGILREISRHSSIEHIDICEIDT 180

Query: 181 MLIDVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIVDAFDPIKPDDEVVR 240
           MLIDVYKKYFPEIAVGYKD RVNLHIIDG  FLSSVP G+YDAIIVDAFDPIKPDDEV+R
Sbjct: 181 MLIDVYKKYFPEIAVGYKDYRVNLHIIDGNVFLSSVPPGSYDAIIVDAFDPIKPDDEVMR 240

Query: 241 SSLFEKVAKALRDGGVLCIQAESLWFHSLDIEILISKCQQVFKGSVQYAWTIVPTYPSGV 295
           S LFEKVAKAL++GGVLCIQAESLWFHSLDI+ILISKC+QVFKGSVQYAWTIVP YPSGV
Sbjct: 241 SKLFEKVAKALKEGGVLCIQAESLWFHSLDIQILISKCKQVFKGSVQYAWTIVPAYPSGV 300

BLAST of Lsi06G015420 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 324.7 bits (831), Expect = 7.6e-89
Identity = 158/289 (54.67%), Postives = 197/289 (68.17%), Query Frame = 0

Query: 41  EEEEDDSINSDALNHPTIPGWFSEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAY 100
           E E  D     A     IPGWFSE  P WPG+AH LKV+KVLFQGKSDYQ ++VFQS+ Y
Sbjct: 71  ETENGDQKKEPACFSTVIPGWFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATY 130

Query: 101 G-----------------------------------KVLLIGGGDGGILREISRHSSIEQ 160
           G                                   KVL+IGGGDGG+LRE++RH+SIEQ
Sbjct: 131 GKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQ 190

Query: 161 IDICEIDTMLIDVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIVDAFDPI 220
           ID+CEID M++DV K++FP++A+GY+D RVNL I DG AFL +   G+YDA+IVD+ DPI
Sbjct: 191 IDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSSDPI 250

Query: 221 KPDDEVVRSSLFEKVAKALRDGGVLCIQAESLWFHSLDIEILISKCQQVFKGSVQYAWTI 280
            P  E+     F+ VA+ALR GGV+C QAESLW H   IE ++S C+++FKGSV YAWT 
Sbjct: 251 GPAKELFEKPFFQSVARALRPGGVVCTQAESLWLHMDIIEDIVSNCREIFKGSVNYAWTS 310

Query: 281 VPTYPSGVIGFLLCSTQGPKVDFKNPINPIDPNENFGVATKPLKFYNSE 295
           VPTYPSGVIGF+LCST+GP VDFK+P+NPID  E+   +  PLKFYN+E
Sbjct: 311 VPTYPSGVIGFMLCSTEGPDVDFKHPLNPID--ESSSKSNGPLKFYNAE 357

BLAST of Lsi06G015420 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 318.9 bits (816), Expect = 4.2e-87
Identity = 166/327 (50.76%), Postives = 212/327 (64.83%), Query Frame = 0

Query: 3   MDANGVLSFSDDLVFKQKKDEVFNNGCSHQHVAVENQKEEEEDDSINSDALNHPTIPGWF 62
           M +    S +D  V + ++ E  NNG +   +  EN   E ++ S  S       IPGWF
Sbjct: 1   MSSTQEASVTDLPVKRPREAEEDNNGGA---METENGGGEIKEPSCMSS-----IIPGWF 60

Query: 63  SEHCPQWPGQAHFLKVDKVLFQGKSDYQSMLVFQSSAYG--------------------- 122
           SE  P WPG+AH LKV+K+LFQGKSDYQ ++VFQS+ YG                     
Sbjct: 61  SEISPMWPGEAHSLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQE 120

Query: 123 --------------KVLLIGGGDGGILREISRHSSIEQIDICEIDTMLIDVYKKYFPEIA 182
                         KVL+IGGGDGG+LRE++RHSS+EQIDICEID M++DV K+YFP +A
Sbjct: 121 MITHLPLCSISNPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVA 180

Query: 183 VGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIVDAFDPIKPDDEVVRSSLFEKVAKALRDG 242
           VGY+D RVNL I DG AFL +   GTYDA+IVD+ DPI P  E+     FE V +ALR G
Sbjct: 181 VGYEDPRVNLIIGDGVAFLKNAAEGTYDAVIVDSSDPIGPAKELFEKPFFESVNRALRPG 240

Query: 243 GVLCIQAESLWFHSLDIEILISKCQQVFKGSVQYAWTIVPTYPSGVIGFLLCSTQGPKVD 295
           GV+C QAESLW H   IE ++S C+ +FKGSV YAWT VPTYPSGVIGF+LCS++GP+VD
Sbjct: 241 GVVCTQAESLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFMLCSSEGPQVD 300

BLAST of Lsi06G015420 vs. TAIR 10
Match: AT5G53120.1 (spermidine synthase 3 )

HSP 1 Score: 290.0 bits (741), Expect = 2.1e-78
Identity = 153/295 (51.86%), Postives = 187/295 (63.39%), Query Frame = 0

Query: 51  DALNHPT-IPGWFSE---------------HCPQWPGQAHFLKVDKVLFQGKSDYQSMLV 110
           DA  H T + GWFSE               + P WPG+AH LKV+KVLF+ KSD+Q +LV
Sbjct: 44  DAKCHSTVVSGWFSEPHPRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLV 103

Query: 111 FQSSAYGK-----------------------------------VLLIGGGDGGILREISR 170
           F+S+ YGK                                   VL++GGGDGG+LREISR
Sbjct: 104 FESATYGKVLVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISR 163

Query: 171 HSSIEQIDICEIDTMLIDVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIV 230
           HSS+E IDICEID M+IDV KK+FPE+AVG+ D RV LHI D   FL   P G YDAIIV
Sbjct: 164 HSSVEVIDICEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIV 223

Query: 231 DAFDPIKPDDEVVRSSLFEKVAKALRDGGVLCIQAESLWFHSLDIEILISKCQQVFKGSV 290
           D+ DP+ P   +V    FE +A+AL+ GGVLC  AES+W H+  IE +IS C+Q FK SV
Sbjct: 224 DSSDPVGPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SV 283

Query: 291 QYAWTIVPTYPSGVIGFLLCSTQGPKVDFKNPINPIDPNENFGVATKPLKFYNSE 295
            YAW+ VPTYPSGVIGF+LCST+GP VDFKNPINPI+  +      + LKFYNS+
Sbjct: 284 HYAWSSVPTYPSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSD 337

BLAST of Lsi06G015420 vs. TAIR 10
Match: AT5G53120.2 (spermidine synthase 3 )

HSP 1 Score: 290.0 bits (741), Expect = 2.1e-78
Identity = 153/295 (51.86%), Postives = 187/295 (63.39%), Query Frame = 0

Query: 51  DALNHPT-IPGWFSE---------------HCPQWPGQAHFLKVDKVLFQGKSDYQSMLV 110
           DA  H T + GWFSE               + P WPG+AH LKV+KVLF+ KSD+Q +LV
Sbjct: 44  DAKCHSTVVSGWFSEPHPRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLV 103

Query: 111 FQSSAYGK-----------------------------------VLLIGGGDGGILREISR 170
           F+S+ YGK                                   VL++GGGDGG+LREISR
Sbjct: 104 FESATYGKVLVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISR 163

Query: 171 HSSIEQIDICEIDTMLIDVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIV 230
           HSS+E IDICEID M+IDV KK+FPE+AVG+ D RV LHI D   FL   P G YDAIIV
Sbjct: 164 HSSVEVIDICEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIV 223

Query: 231 DAFDPIKPDDEVVRSSLFEKVAKALRDGGVLCIQAESLWFHSLDIEILISKCQQVFKGSV 290
           D+ DP+ P   +V    FE +A+AL+ GGVLC  AES+W H+  IE +IS C+Q FK SV
Sbjct: 224 DSSDPVGPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SV 283

Query: 291 QYAWTIVPTYPSGVIGFLLCSTQGPKVDFKNPINPIDPNENFGVATKPLKFYNSE 295
            YAW+ VPTYPSGVIGF+LCST+GP VDFKNPINPI+  +      + LKFYNS+
Sbjct: 284 HYAWSSVPTYPSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSD 337

BLAST of Lsi06G015420 vs. TAIR 10
Match: AT5G53120.3 (spermidine synthase 3 )

HSP 1 Score: 290.0 bits (741), Expect = 2.1e-78
Identity = 153/295 (51.86%), Postives = 187/295 (63.39%), Query Frame = 0

Query: 51  DALNHPT-IPGWFSE---------------HCPQWPGQAHFLKVDKVLFQGKSDYQSMLV 110
           DA  H T + GWFSE               + P WPG+AH LKV+KVLF+ KSD+Q +LV
Sbjct: 44  DAKCHSTVVSGWFSEPHPRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLV 103

Query: 111 FQSSAYGK-----------------------------------VLLIGGGDGGILREISR 170
           F+S+ YGK                                   VL++GGGDGG+LREISR
Sbjct: 104 FESATYGKVLVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISR 163

Query: 171 HSSIEQIDICEIDTMLIDVYKKYFPEIAVGYKDSRVNLHIIDGTAFLSSVPAGTYDAIIV 230
           HSS+E IDICEID M+IDV KK+FPE+AVG+ D RV LHI D   FL   P G YDAIIV
Sbjct: 164 HSSVEVIDICEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIV 223

Query: 231 DAFDPIKPDDEVVRSSLFEKVAKALRDGGVLCIQAESLWFHSLDIEILISKCQQVFKGSV 290
           D+ DP+ P   +V    FE +A+AL+ GGVLC  AES+W H+  IE +IS C+Q FK SV
Sbjct: 224 DSSDPVGPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SV 283

Query: 291 QYAWTIVPTYPSGVIGFLLCSTQGPKVDFKNPINPIDPNENFGVATKPLKFYNSE 295
            YAW+ VPTYPSGVIGF+LCST+GP VDFKNPINPI+  +      + LKFYNS+
Sbjct: 284 HYAWSSVPTYPSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSD 337

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZS452.5e-9255.92Spermidine synthase OS=Solanum lycopersicum OX=4081 GN=SPDSYN PE=2 SV=1[more]
O486592.5e-9256.99Spermidine synthase 2 OS=Hyoscyamus niger OX=4079 PE=2 SV=1[more]
O821473.2e-9257.14Spermidine synthase OS=Coffea arabica OX=13443 PE=2 SV=1[more]
O486582.7e-9155.26Spermidine synthase 1 OS=Hyoscyamus niger OX=4079 PE=2 SV=1[more]
Q965572.7e-9154.61Spermidine synthase 2 OS=Datura stramonium OX=4076 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JLE91.4e-13876.29spermidine synthase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485649 PE=3 SV=1[more]
A0A6J1E1D21.4e-13876.60spermidine synthase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111429923 PE=3 SV... [more]
A0A0A0LP682.6e-13776.18PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G005540 PE=3 S... [more]
A0A1S3BVF43.4e-13775.07spermidine synthase 2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493919 PE... [more]
A0A5A7UWC72.1e-13474.28Spermidine synthase 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
Match NameE-valueIdentityDescription
XP_038879915.11.9e-14573.99spermidine synthase 2-like isoform X2 [Benincasa hispida][more]
XP_038879914.16.6e-14368.84spermidine synthase 2-like isoform X1 [Benincasa hispida][more]
XP_022988394.12.9e-13876.29spermidine synthase 1-like [Cucurbita maxima][more]
XP_022921767.12.9e-13876.60spermidine synthase 1-like [Cucurbita moschata][more]
XP_011657853.14.9e-13876.32spermidine synthase 2 isoform X1 [Cucumis sativus] >KAE8652405.1 hypothetical pr... [more]
Match NameE-valueIdentityDescription
AT1G23820.17.6e-8954.67spermidine synthase 1 [more]
AT1G70310.14.2e-8750.76spermidine synthase 2 [more]
AT5G53120.12.1e-7851.86spermidine synthase 3 [more]
AT5G53120.22.1e-7851.86spermidine synthase 3 [more]
AT5G53120.32.1e-7851.86spermidine synthase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 86..295
e-value: 4.7E-64
score: 218.0
NoneNo IPR availablePFAMPF01564Spermine_synthcoord: 97..270
e-value: 6.6E-50
score: 169.1
NoneNo IPR availablePANTHERPTHR11558:SF44SPERMIDINE SYNTHASEcoord: 54..104
coord: 102..294
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 124..213
e-value: 3.44987E-5
score: 40.1059
IPR035246Spermidine synthase, tetramerisation domainPFAMPF17284Spermine_synt_Ncoord: 60..107
e-value: 1.8E-15
score: 56.5
IPR001045Spermidine/spermine synthasesPANTHERPTHR11558SPERMIDINE/SPERMINE SYNTHASEcoord: 102..294
IPR001045Spermidine/spermine synthasesPANTHERPTHR11558SPERMIDINE/SPERMINE SYNTHASEcoord: 54..104
IPR001045Spermidine/spermine synthasesHAMAPMF_00198Spermidine_synthcoord: 60..293
score: 19.060966
IPR030373Polyamine biosynthesis domain, conserved sitePROSITEPS01330PABS_1coord: 103..116
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 102..261
score: 45.743484
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 59..101
score: 8.839154
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 59..293

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G015420.1Lsi06G015420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006596 polyamine biosynthetic process
molecular_function GO:0016740 transferase activity
molecular_function GO:0003824 catalytic activity