Lsi06G014970 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G014970
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	protein NUCLEAR FUSION DEFECTIVE 4
Location	chr06: 25432208 .. 25435438 (+)
RNA-Seq Expression	Lsi06G014970
Synteny	Lsi06G014970

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGAAAACCGCCAAGAAAAATGATGATGGGAAGTACCAACTATGGCCCACGTAGTAAACCGATGCATTGCCCGCCCTCATTAGTTCGGTGAAGGGAGAACAAATATGAGGTCGTCTTCTACGATACGGAAATGATTCCCATTCTGCTACAACGAACAACTAGAACCAGAAAGATTACCTACCGCCGGAGATGGAAGTTTCTGGGTTTATGCCCAACAAATGGATTGCAACCGCCCTTGGAATTTGGATTCAATGCATCTGCGGTGCTTCCTACACCTTCAGCATCTATTCTTCCGCCCTCAAGTCCACGCAGGCCTACGATCAATCCACCCTCGACACCGTTTCCGTTTTCAAAGACATTGGCGCCAACGCCGGCATAATTTCTGGTTTTCTTTACTCTGCCGTTGCTCCCCCCAATCCCCGCCGTGCTTTTGCCGGGCCATGGGTGGTTCACGCCGCGGGAGCGATTCAGTGCTTCCTCGGATATATTTTCATTTGGGCCGCTGTCTCTGGAGTGATAGATCGGCCGCCGGTGCCGGCGATGTGCTTCTTCATGTTTCTGGCTGCACATGCGCAAACGTTTTTCAACACGGCGAATGTGGTGACTGGCGTGCACAATTTTGCGCACTACAGCGGGACGATTGTGGGAATCATGAAGGTACGAACGAAAACCCTTGGTTATCTTCATTATTGTTATGCAGATTGTTAGGGCTCTGGATTTTCCTTTGATTTCTCCCATTTTCTGAACCCTTATTGTATTTTTCTCTCTGGGAGGTTTGGTCATGGTGAGTGCTATTTAGATACTGTAAAGAATCCATGTACTCTAAATAAAATCTATGTCCTGGAGATGAACTTTGTTTAGCTGGCCTCATTCTTTACAAGAAATTTTATCTTGAAGCATAGAAATGGAAGAAGACGGTGGAGCCACCAAACCAATTTGTCTTTCCTTCCTGCTCTGTTCTTCTCTGCCATAATCTATTTTTGATCAAGAAGGTGGCCAGATCAAAACTCCAAGTTATTGTCTCACGATGGTCCTGGAAATATGAGATTAAACATAAACATAATCATAACCATTCAAATCTAAATGGTTATCAAATGAGACCTAATTAATCGAAAAATCTGGTGTTTAAGCCTAGTGTTATTTACATTACTTGTAAGTATTAGTTTTGTGCTAATTTCTAAACCCAGAAAGTGGTAGTGTTAAAACCATATAGAAGAAGATAAAATATGTGCTGAACTATGAACTTAACTTAAAAATTTAGGTGTATTCTAGACTGCACATATTTTTGTGCATCCCACCAAATTGACCAATCATAATTTTCCACACGACAAATTGTTCTTTGTTCTTTTTTGAAATATTCTAATTCAATTTTTATGTGTGATGAGATTGAGATGTACAAAGATAGGTGCATCTTGTAATACACCTAAGTATTGCTCCTAACTTAAAAGAGTTTAACAGTAATTTTTAGTGACTATATCTGATTCTCATTTATTCCGTGGAAAAGGGGATCACTATCTGAATAGTTTCAAGGTTTTTTATTTGACAAAGTGGGATCACTATTGCTTTTCTTTGGTACAAAACTAAGCACCCCTTTTTCTCACTTCAACTTATCTTTTTTATTGTAATCACTTTTTGGTGTTGGGTTTATCCCATTATTTCATTCATCAATGAAATGTTCCTATTACCCAAAAAGAAAAAGAAGTTGTAGTTGCCTTGTTGAATCTGAGAGCAGATTCCAAGGATTTTCCCCAGATTTTGTATCTTGAATGTGCTATTTTTGAGTTCATATCTTGTGTCTCCTTTTTGTTGGATAGGGATATCTTGGTCTCAGTGGAGCACTGCTAATCCAAGTTTATAATACGACTTGCAATGGAGATCCAAGCAATTTTCTCCTCATGCTGGCGGTTTTGCCCACACTTCTTTCCATCATGTTCATGTGGTTTGTTAGAATTGACAAAACAGAGTCTAGTAATGAGATGAAGCATTTGAACTCATTGTCTGCACTTGCTGTGATAGTTGCTTTTTACCTAATGGTTGTCATCATCTTGAACAACACTTTCACCCTTTCATCATGGACTCGTTTCTTCACATTCTCCATACTCTTGATTCTCCTTGCTGCTCCTCTTGGAATCGCCCTTAATGCTCAGAGGGAGGACTTTAGAGAGTCATCTCCATCATTGTTCACTGAAAAAAGTCATGTTGTTAACAAACCCAAGTCAGTGGATGCAGAGGATTCGGTTGAATATCATGAGTTGCCCGGGGATGAGAGCCAGATAGTGGCTGTTTCGAACTCTAGAATCCCTCAAGCCATGAATGTCTTGGAAGCTATACGCACTATAAACTTCTGGCTGTTGTTTCTTGCCATGGTGTGTGGAATGGGTTCTGGATTAGCCACAATAAACAACATGAGCCAACTTGGCCAGTCTCTTGGTTACACAGCAACAGAAACAAAGACATTTGTTTCATTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCCGGTTACACCTCTGATTTTCTTCTCCACTCATATGGCTGGGCGAGGCCATTGTTAATGGCGATTACTCTACTGATAATGAGTGGCGGCCACATCGTGATTGCCTCTGGTTTCTCTGGAAATCTGTATGTGGGTTCAATTCTTGTTGGCATATGCTACGGCTCACAGTGGTCATTGATGCCCACAATCACTTCAGAGATATTTGGTTTAGAGCATATGGGTACTATTTTCAACACCATAGCCATAGCCAGCCCTTTGGGGTCTTACATTTTTTCTGTTAGAGTTATCGGATACATTTACGACAGAGAGGCTGCCGGAGAACATGGCTCGTGCTCCGGCACACACTGTTTTGTGGTATCTTTTCTTGTCATGGCCGTCGTGGCTTTCTTGGGATTTCTTGTTGCTGCTGCTCTCTTCTTTCGAACCAGGAGATTTTACCGGCTGGCTTTGCAAAGAAGAGTTGAATGTCTCAGTTAAAGGCGCGCTAGAGAGAAGTACGATAAGTAAGATGATTGTTTATAGATTTTATAGATTTCTGGAAGGATTTTGCTGAGTGTAAAGAAATTTGTTGTCTTGCTTCATAGAAGTTTATAACAGTATATCACTCAAGTTATATTTTAAAAATGTTTTATTGTATCATAGGCTGATCAAGAGACCAACCGTATCATTTTTAGTACATATGTTTTATTTTATTTTATTTTTAATAAAAATGATGTTGGTTATGGGGGAA

mRNA sequence

AGGAAAACCGCCAAGAAAAATGATGATGGGAAGTACCAACTATGGCCCACGTAGTAAACCGATGCATTGCCCGCCCTCATTAGTTCGGTGAAGGGAGAACAAATATGAGGTCGTCTTCTACGATACGGAAATGATTCCCATTCTGCTACAACGAACAACTAGAACCAGAAAGATTACCTACCGCCGGAGATGGAAGTTTCTGGGTTTATGCCCAACAAATGGATTGCAACCGCCCTTGGAATTTGGATTCAATGCATCTGCGGTGCTTCCTACACCTTCAGCATCTATTCTTCCGCCCTCAAGTCCACGCAGGCCTACGATCAATCCACCCTCGACACCGTTTCCGTTTTCAAAGACATTGGCGCCAACGCCGGCATAATTTCTGGTTTTCTTTACTCTGCCGTTGCTCCCCCCAATCCCCGCCGTGCTTTTGCCGGGCCATGGGTGGTTCACGCCGCGGGAGCGATTCAGTGCTTCCTCGGATATATTTTCATTTGGGCCGCTGTCTCTGGAGTGATAGATCGGCCGCCGGTGCCGGCGATGTGCTTCTTCATGTTTCTGGCTGCACATGCGCAAACGTTTTTCAACACGGCGAATGTGGTGACTGGCGTGCACAATTTTGCGCACTACAGCGGGACGATTGTGGGAATCATGAAGGGATATCTTGGTCTCAGTGGAGCACTGCTAATCCAAGTTTATAATACGACTTGCAATGGAGATCCAAGCAATTTTCTCCTCATGCTGGCGGTTTTGCCCACACTTCTTTCCATCATGTTCATGTGGTTTGTTAGAATTGACAAAACAGAGTCTAGTAATGAGATGAAGCATTTGAACTCATTGTCTGCACTTGCTGTGATAGTTGCTTTTTACCTAATGGTTGTCATCATCTTGAACAACACTTTCACCCTTTCATCATGGACTCGTTTCTTCACATTCTCCATACTCTTGATTCTCCTTGCTGCTCCTCTTGGAATCGCCCTTAATGCTCAGAGGGAGGACTTTAGAGAGTCATCTCCATCATTGTTCACTGAAAAAAGTCATGTTGTTAACAAACCCAAGTCAGTGGATGCAGAGGATTCGGTTGAATATCATGAGTTGCCCGGGGATGAGAGCCAGATAGTGGCTGTTTCGAACTCTAGAATCCCTCAAGCCATGAATGTCTTGGAAGCTATACGCACTATAAACTTCTGGCTGTTGTTTCTTGCCATGGTGTGTGGAATGGGTTCTGGATTAGCCACAATAAACAACATGAGCCAACTTGGCCAGTCTCTTGGTTACACAGCAACAGAAACAAAGACATTTGTTTCATTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCCGGTTACACCTCTGATTTTCTTCTCCACTCATATGGCTGGGCGAGGCCATTGTTAATGGCGATTACTCTACTGATAATGAGTGGCGGCCACATCGTGATTGCCTCTGGTTTCTCTGGAAATCTGTATGTGGGTTCAATTCTTGTTGGCATATGCTACGGCTCACAGTGGTCATTGATGCCCACAATCACTTCAGAGATATTTGGTTTAGAGCATATGGGTACTATTTTCAACACCATAGCCATAGCCAGCCCTTTGGGGTCTTACATTTTTTCTGTTAGAGTTATCGGATACATTTACGACAGAGAGGCTGCCGGAGAACATGGCTCGTGCTCCGGCACACACTGTTTTGTGGTATCTTTTCTTGTCATGGCCGTCGTGGCTTTCTTGGGATTTCTTGTTGCTGCTGCTCTCTTCTTTCGAACCAGGAGATTTTACCGGCTGGCTTTGCAAAGAAGAGTTGAATGTCTCAGTTAAAGGCGCGCTAGAGAGAAGTACGATAAGTAAGATGATTGTTTATAGATTTTATAGATTTCTGGAAGGATTTTGCTGAGTGTAAAGAAATTTGTTGTCTTGCTTCATAGAAGTTTATAACAGTATATCACTCAAGTTATATTTTAAAAATGTTTTATTGTATCATAGGCTGATCAAGAGACCAACCGTATCATTTTTAGTACATATGTTTTATTTTATTTTATTTTTAATAAAAATGATGTTGGTTATGGGGGAA

Coding sequence (CDS)

ATGGAAGTTTCTGGGTTTATGCCCAACAAATGGATTGCAACCGCCCTTGGAATTTGGATTCAATGCATCTGCGGTGCTTCCTACACCTTCAGCATCTATTCTTCCGCCCTCAAGTCCACGCAGGCCTACGATCAATCCACCCTCGACACCGTTTCCGTTTTCAAAGACATTGGCGCCAACGCCGGCATAATTTCTGGTTTTCTTTACTCTGCCGTTGCTCCCCCCAATCCCCGCCGTGCTTTTGCCGGGCCATGGGTGGTTCACGCCGCGGGAGCGATTCAGTGCTTCCTCGGATATATTTTCATTTGGGCCGCTGTCTCTGGAGTGATAGATCGGCCGCCGGTGCCGGCGATGTGCTTCTTCATGTTTCTGGCTGCACATGCGCAAACGTTTTTCAACACGGCGAATGTGGTGACTGGCGTGCACAATTTTGCGCACTACAGCGGGACGATTGTGGGAATCATGAAGGGATATCTTGGTCTCAGTGGAGCACTGCTAATCCAAGTTTATAATACGACTTGCAATGGAGATCCAAGCAATTTTCTCCTCATGCTGGCGGTTTTGCCCACACTTCTTTCCATCATGTTCATGTGGTTTGTTAGAATTGACAAAACAGAGTCTAGTAATGAGATGAAGCATTTGAACTCATTGTCTGCACTTGCTGTGATAGTTGCTTTTTACCTAATGGTTGTCATCATCTTGAACAACACTTTCACCCTTTCATCATGGACTCGTTTCTTCACATTCTCCATACTCTTGATTCTCCTTGCTGCTCCTCTTGGAATCGCCCTTAATGCTCAGAGGGAGGACTTTAGAGAGTCATCTCCATCATTGTTCACTGAAAAAAGTCATGTTGTTAACAAACCCAAGTCAGTGGATGCAGAGGATTCGGTTGAATATCATGAGTTGCCCGGGGATGAGAGCCAGATAGTGGCTGTTTCGAACTCTAGAATCCCTCAAGCCATGAATGTCTTGGAAGCTATACGCACTATAAACTTCTGGCTGTTGTTTCTTGCCATGGTGTGTGGAATGGGTTCTGGATTAGCCACAATAAACAACATGAGCCAACTTGGCCAGTCTCTTGGTTACACAGCAACAGAAACAAAGACATTTGTTTCATTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCCGGTTACACCTCTGATTTTCTTCTCCACTCATATGGCTGGGCGAGGCCATTGTTAATGGCGATTACTCTACTGATAATGAGTGGCGGCCACATCGTGATTGCCTCTGGTTTCTCTGGAAATCTGTATGTGGGTTCAATTCTTGTTGGCATATGCTACGGCTCACAGTGGTCATTGATGCCCACAATCACTTCAGAGATATTTGGTTTAGAGCATATGGGTACTATTTTCAACACCATAGCCATAGCCAGCCCTTTGGGGTCTTACATTTTTTCTGTTAGAGTTATCGGATACATTTACGACAGAGAGGCTGCCGGAGAACATGGCTCGTGCTCCGGCACACACTGTTTTGTGGTATCTTTTCTTGTCATGGCCGTCGTGGCTTTCTTGGGATTTCTTGTTGCTGCTGCTCTCTTCTTTCGAACCAGGAGATTTTACCGGCTGGCTTTGCAAAGAAGAGTTGAATGTCTCAGTTAA

Protein sequence

MEVSGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGANAGIISGFLYSAVAPPNPRRAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCFFMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFFTFSILLILLAAPLGIALNAQREDFRESSPSLFTEKSHVVNKPKSVDAEDSVEYHELPGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQRRVECLS

Homology

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BLAST of Lsi06G014970 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 5.5e-29
Identity = 144/560 (25.71%), Postives = 245/560 (43.75%), Query Frame = 0


Query: 10  KWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGANAGIISGFLY 69
           KW      IWIQ   G ++ FS YSS LKS     Q  L+ ++V  D+G   G  SG   
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 70  SAVAPPNPRRAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCFFMFLAAHAQ 129
                      +    VV  A A   F+GY   W  ++ +I   P   +     LA  + 
Sbjct: 103 ----------GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITL-PYSLVFLCCLLAGLSI 162

Query: 130 TFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLLMLAVLP 189
            +FNTA  +  + +F +     + +   + G+S AL    +N       + +LL+ +++P
Sbjct: 163 CWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVP 222

Query: 190 TLLSIMFMWFV----RIDKTESSNEMKH----LNSLSALAVIVAFYLMVVIILNNTFTLS 249
            ++S   ++ V     +D T   +  +H       L+ LAVI +F+L    +L+++ T S
Sbjct: 223 LVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHL----LLSSSSTSS 282

Query: 250 SWTRFFTFSILLIL-LAAPLGI--------ALNAQREDFRESSPSLFTEKSHVVNKPKSV 309
           +   F    +LL+  L APL +         +NA+     ESS  +      + N+  SV
Sbjct: 283 ARLNFIGAVVLLVFPLCAPLLVYARDYFLPVINARLN--HESSGYVMLNIDELKNQKTSV 342

Query: 310 DAEDSVEYHELPGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATIN 369
            ++   E H     E   V + +    + +     I  + FWL ++A  CG   GL   N
Sbjct: 343 SSKTGYE-HMGTAKEGNTVRLGDEHSFRLL-----ISRLEFWLYYIAYFCGGTIGLVYSN 402

Query: 370 NMSQLGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIM 429
           N+ Q+ QSLG  +T   T V+++S ++F GR       DF+   +   R    AI LL  
Sbjct: 403 NLGQIAQSLGQNST---TLVTIYSSFSFFGRL-LSAAPDFMHKRFRLTRTGWFAIALLPT 462

Query: 430 SGGHIVIASGFSGN--LYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASP 489
                ++A   S    L   + L+G+  G  ++   +ITS++FG   +G   N +    P
Sbjct: 463 PIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIP 522

Query: 490 LGSYIFSVRVIGYIYDREAA-------GEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAAL 544
           +GS ++   +   IY+  A+        +   C G  C+  +F+    ++ LG + + +L
Sbjct: 523 IGSLLYGY-IAASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSL 574

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BLAST of Lsi06G014970 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.5e-10
Identity = 58/206 (28.16%), Postives = 111/206 (53.88%), Query Frame = 0


Query: 334 WLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTF---VSLWSIWNFLGRFGAGYTS 393
           WL  L  V   G      NNM  +  ++     ++ +F   VSL++ ++ + R   G++S
Sbjct: 275 WLFLLCFVFIGGPFEMFQNNMGAILDTVTVENADSPSFSTHVSLFATFSTVSRLVVGFSS 334

Query: 394 DFLLHSYGWARPLLMAITLLIMSGGHIVIASG----FSGNLY--VGSILVGICYGSQWSL 453
           + +      +RP+L+++  L+ +  H+++ SG    F    Y  V +I+ G  YGS ++L
Sbjct: 335 EAMESHV--SRPVLLSVIALVAACIHLMVPSGIFTVFDNAKYFSVVTIVNGFSYGSSFTL 394

Query: 454 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEHGS----CSGTH 513
           +PTI ++++G+ ++GTI+ +  +A  +GS  + + +   +YD  +    GS    CSG H
Sbjct: 395 VPTIVTKVWGIANLGTIWGSFILALAVGSLGYGL-LFAKVYDAASEVGVGSMSQVCSGVH 454

Query: 514 CFVVSFLVMAV-VAFLGFLVAAALFF 526
           C+ ++F++    +AF     AAA+FF
Sbjct: 455 CYGLTFVITGTGLAF----AAAAVFF 473

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BLAST of Lsi06G014970 vs. ExPASy Swiss-Prot
Match: Q96TW9 (Probable transporter MCH1 OS=Wickerhamomyces anomalus OX=4927 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 5.3e-08
Identity = 50/185 (27.03%), Postives = 92/185 (49.73%), Query Frame = 0


Query: 325 LEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 384
           L+ I+ I+ ++L  +++  +G     I NM  L +++   +  +   V++ ++++ L R 
Sbjct: 256 LKFIKDISTYVLLFSLLLSIGPSEMYITNMGSLVKAITPNSLISDQ-VAIHAVFSTLSRL 315

Query: 385 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASG--FSGNLYVGSILVGICYGSQW 444
             G  SDFL+ +Y  +R  L+   +++     I IA+        Y+ S L G  YG  +
Sbjct: 316 SLGALSDFLVTNYQISRSWLLLSIIVLGFFTQIFIATSTFVKDQYYIISALSGFSYGGLF 375

Query: 445 SLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEHGSCSGTHCF 504
           +L PT+   I+G E  G+ + +  IA  +GS  F + V G +YD  A G     +  +C 
Sbjct: 376 TLYPTVIFSIWGPEIFGSAWGSFMIAPAIGSTTFGM-VFGLVYD-SACGVFAESTTGNCV 435

Query: 505 VVSFL 508
            + FL
Sbjct: 436 SLVFL 437

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BLAST of Lsi06G014970 vs. ExPASy Swiss-Prot
Match: Q6FWD4 (Probable transporter MCH1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.2e-07
Identity = 52/211 (24.64%), Postives = 99/211 (46.92%), Query Frame = 0


Query: 294 AEDSVEYHELPGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINN 353
           +++ VE H    ++S+++  +++   Q M +++  R    ++    + C +G     I N
Sbjct: 244 SDNDVENHYDDNEQSRLLHATHA---QQMTLMKVFRDPVLYIFGATIFCALGPLEMFIAN 303

Query: 354 MSQLGQSL--GYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLH---SYGWARPLLMAIT 413
           M  L   L  G+    +   +S++++ + L R G G T D+      S  W   L + + 
Sbjct: 304 MGSLTNVLAGGHEPAMSSALLSIYALTSTLTRLGTGLTVDYFNKRQLSVKWILLLFLVVG 363

Query: 414 LLIMSGGHIVIASGFSGN---------LYVGSILVGICYGSQWSLMPTITSEIFGLEHMG 473
           L+     +++  S    +          Y+G I+ GI YG  +++ PTIT  ++G +  G
Sbjct: 364 LVTQGKIYMLSMSSLDHSHMVTINRKLFYIG-IMQGIAYGGLFTIYPTITLMVWGEKMFG 423

Query: 474 TIFNTIAIASPLGSYIFSVRVIGYIYDREAA 491
           T + T+ IA  LGS + S  +   +YD E A
Sbjct: 424 TAYGTLMIAPALGSAL-SCLIYADVYDSECA 449

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BLAST of Lsi06G014970 vs. ExPASy TrEMBL
Match: A0A5A7UT75 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002270 PE=4 SV=1)

HSP 1 Score: 995.7 bits (2573), Expect = 7.4e-287
Identity = 506/543 (93.19%), Postives = 522/543 (96.13%), Query Frame = 0


Query: 1   MEVSGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGAN 60
           ME S FM NKW AT LGIWIQCICGASYTFSIYSSALKSTQ+YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVAPPNPRRAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGIISGFLYSAV P NPRRAFAGPW+VHAAGAIQ FLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFA+YSGTIVGIMKGYLGLSGALLIQVYNTTCN DPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTL 240
           FLLMLAVLPT+LSIMFMWFVRIDKTES NEMKHLNSLSALAVIVAFYLMVVIILNN F+L
Sbjct: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLGIALNAQREDFRESSPSLFTEKSHVVNKPKSVDAEDSVEY 300
           SSWTR+FTFSILLILLAAPLGIA+NAQ+EDFR S PSL TE+SHVVNKP+ +DAEDSVEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEY 300

Query: 301 HELPGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP +E QI+ VSNSR PQ MNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPREEDQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFL H+YGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAAGEHG+CSGTHCFVVSFLVMA+VAFLGFLVAAALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
           CLS
Sbjct: 541 CLS 543

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BLAST of Lsi06G014970 vs. ExPASy TrEMBL
Match: A0A1S3BWC2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 SV=1)

HSP 1 Score: 995.7 bits (2573), Expect = 7.4e-287
Identity = 506/543 (93.19%), Postives = 522/543 (96.13%), Query Frame = 0


Query: 1   MEVSGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGAN 60
           ME S FM NKW AT LGIWIQCICGASYTFSIYSSALKSTQ+YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVAPPNPRRAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGIISGFLYSAV P NPRRAFAGPW+VHAAGAIQ FLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFA+YSGTIVGIMKGYLGLSGALLIQVYNTTCN DPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTL 240
           FLLMLAVLPT+LSIMFMWFVRIDKTES NEMKHLNSLSALAVIVAFYLMVVIILNN F+L
Sbjct: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLGIALNAQREDFRESSPSLFTEKSHVVNKPKSVDAEDSVEY 300
           SSWTR+FTFSILLILLAAPLGIA+NAQ+EDFR S PSL TE+SHVVNKP+ +DAEDSVEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEY 300

Query: 301 HELPGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP +E QI+ VSNSR PQ MNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPREEDQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFL H+YGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAAGEHG+CSGTHCFVVSFLVMA+VAFLGFLVAAALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
           CLS
Sbjct: 541 CLS 543

[Close]

BLAST of Lsi06G014970 vs. ExPASy TrEMBL
Match: A0A0A0LUE8 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G008470 PE=4 SV=1)

HSP 1 Score: 975.7 bits (2521), Expect = 7.9e-281
Identity = 497/543 (91.53%), Postives = 518/543 (95.40%), Query Frame = 0


Query: 1   MEVSGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGAN 60
           M+ S FM NKWIAT LGIWIQCICGASYTFSIYSSALKSTQ+YDQSTLDTVSVFKDIGAN
Sbjct: 1   MDPSNFMSNKWIATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVAPPNPRRAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGIISGFLYSAV P NPRRAFAGPW+VHAAGAIQ FLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPFNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFA+YSGTIVGIMKGYLGLSGALLIQVYNTTCN DPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNEDPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTL 240
           FLLMLAVLPT+LS+MFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNN F+L
Sbjct: 181 FLLMLAVLPTVLSVMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLGIALNAQREDFRESSPSLFTEKSHVVNKPKSVDAEDSVEY 300
           SSWTR+FTFSILLILLAAPLGIA+NAQ+EDFR SS SL  EKSHVVNKP+S+DAEDSVEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSSSSLIAEKSHVVNKPESIDAEDSVEY 300

Query: 301 HELPGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP +E+QI+ VSN+R PQ MNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPREENQIMVVSNTRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420
           LGYT TETKTFVSLWSIWNFLGRFGAGYTSDFL H+YGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTETETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAA EHG+CSG HCFVVSF VMA+VAFLGFLVAAALFFRTRRFY+LA QRRV 
Sbjct: 481 IGYIYDREAAREHGACSGIHCFVVSFFVMAIVAFLGFLVAAALFFRTRRFYQLASQRRVN 540

Query: 541 CLS 544
            +S
Sbjct: 541 RVS 543

[Close]

BLAST of Lsi06G014970 vs. ExPASy TrEMBL
Match: A0A6J1IA87 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471070 PE=4 SV=1)

HSP 1 Score: 973.8 bits (2516), Expect = 3.0e-280
Identity = 493/543 (90.79%), Postives = 517/543 (95.21%), Query Frame = 0


Query: 1   MEVSGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGAN 60
           MEV GFM NKW+ATA+GIWIQCICGASYTFSIYSSALKSTQ YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVAPPNPRRAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AG+ISGFLYSAV P + RR+  GPWVVHAAGAIQCFLGYIFIWAAVSG+IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPASRRRSSVGPWVVHAAGAIQCFLGYIFIWAAVSGLIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMKGYLGLSGALLIQVYNT CNGDPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMKGYLGLSGALLIQVYNTACNGDPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTL 240
           F+LMLAV PTLLSIMFMWFVRID+TES NEMKHLNSLSALAVIVAFYLMVVII NNTF+L
Sbjct: 181 FVLMLAVFPTLLSIMFMWFVRIDRTESRNEMKHLNSLSALAVIVAFYLMVVIIFNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLGIALNAQREDFRESSPSLFTEKSHVVNKPKSVDAEDSVEY 300
           SSWTRF TFSILL+LL APLGIA+NAQREDFR SSPS FTE++ V+NKPKS +AEDSVEY
Sbjct: 241 SSWTRFVTFSILLVLLVAPLGIAINAQREDFRMSSPSFFTEENPVLNKPKSANAEDSVEY 300

Query: 301 HELPGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP DE Q +A +NSR P+AMNVLEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPSDELQTMAAANSRAPRAMNVLEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAA +HGSCSGTHCFVVSFLVMA+VAFLG LVAAALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAEKHGSCSGTHCFVVSFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
            LS
Sbjct: 541 RLS 543

[Close]

BLAST of Lsi06G014970 vs. ExPASy TrEMBL
Match: A0A6J1F604 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111441144 PE=4 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 1.1e-277
Identity = 490/543 (90.24%), Postives = 512/543 (94.29%), Query Frame = 0


Query: 1   MEVSGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGAN 60
           MEV GFM NKW+ATA+GIWIQCICGASYTFSIYSSALKSTQ YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVAPPNPRRAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AG+ISGFLYSAV P   RR+ AGPWVVHAAGAIQCFLGYIFIWAAVSG IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPTTRRRSSAGPWVVHAAGAIQCFLGYIFIWAAVSGFIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMKGYLGLSGALLIQVYNT CNG+PSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMKGYLGLSGALLIQVYNTACNGEPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTL 240
           FLLMLAV PTLLSI FMWFVRID+TES NEMKHLNSLSALAVIVAFYLMV II NNTF+L
Sbjct: 181 FLLMLAVFPTLLSITFMWFVRIDRTESRNEMKHLNSLSALAVIVAFYLMVAIIFNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLGIALNAQREDFRESSPSLFTEKSHVVNKPKSVDAEDSVEY 300
           SSWTRF TFSILL+LL APLGIA+NAQRED R SSPS FTE++ V+NKPKS +AEDSVEY
Sbjct: 241 SSWTRFVTFSILLVLLVAPLGIAINAQREDLRMSSPSFFTEENPVLNKPKSANAEDSVEY 300

Query: 301 HELPGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP DE Q +A +NSR P+AMNVLEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPSDELQTMAAANSRAPRAMNVLEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SG SGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGLSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAA +HGSCSGTHCFVVSFLVMA+VAFLG LVAAALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAEKHGSCSGTHCFVVSFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
            LS
Sbjct: 541 RLS 543

[Close]

BLAST of Lsi06G014970 vs. NCBI nr
Match: XP_008453166.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo] >KAA0057917.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ98605.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 995.7 bits (2573), Expect = 1.5e-286
Identity = 506/543 (93.19%), Postives = 522/543 (96.13%), Query Frame = 0


Query: 1   MEVSGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGAN 60
           ME S FM NKW AT LGIWIQCICGASYTFSIYSSALKSTQ+YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVAPPNPRRAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGIISGFLYSAV P NPRRAFAGPW+VHAAGAIQ FLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFA+YSGTIVGIMKGYLGLSGALLIQVYNTTCN DPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTL 240
           FLLMLAVLPT+LSIMFMWFVRIDKTES NEMKHLNSLSALAVIVAFYLMVVIILNN F+L
Sbjct: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLGIALNAQREDFRESSPSLFTEKSHVVNKPKSVDAEDSVEY 300
           SSWTR+FTFSILLILLAAPLGIA+NAQ+EDFR S PSL TE+SHVVNKP+ +DAEDSVEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEY 300

Query: 301 HELPGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP +E QI+ VSNSR PQ MNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPREEDQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFL H+YGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAAGEHG+CSGTHCFVVSFLVMA+VAFLGFLVAAALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
           CLS
Sbjct: 541 CLS 543

[Close]

BLAST of Lsi06G014970 vs. NCBI nr
Match: XP_011659929.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN63641.1 hypothetical protein Csa_013553 [Cucumis sativus])

HSP 1 Score: 975.7 bits (2521), Expect = 1.6e-280
Identity = 497/543 (91.53%), Postives = 518/543 (95.40%), Query Frame = 0


Query: 1   MEVSGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGAN 60
           M+ S FM NKWIAT LGIWIQCICGASYTFSIYSSALKSTQ+YDQSTLDTVSVFKDIGAN
Sbjct: 1   MDPSNFMSNKWIATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVAPPNPRRAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGIISGFLYSAV P NPRRAFAGPW+VHAAGAIQ FLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPFNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFA+YSGTIVGIMKGYLGLSGALLIQVYNTTCN DPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNEDPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTL 240
           FLLMLAVLPT+LS+MFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNN F+L
Sbjct: 181 FLLMLAVLPTVLSVMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLGIALNAQREDFRESSPSLFTEKSHVVNKPKSVDAEDSVEY 300
           SSWTR+FTFSILLILLAAPLGIA+NAQ+EDFR SS SL  EKSHVVNKP+S+DAEDSVEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSSSSLIAEKSHVVNKPESIDAEDSVEY 300

Query: 301 HELPGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP +E+QI+ VSN+R PQ MNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPREENQIMVVSNTRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420
           LGYT TETKTFVSLWSIWNFLGRFGAGYTSDFL H+YGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTETETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAA EHG+CSG HCFVVSF VMA+VAFLGFLVAAALFFRTRRFY+LA QRRV 
Sbjct: 481 IGYIYDREAAREHGACSGIHCFVVSFFVMAIVAFLGFLVAAALFFRTRRFYQLASQRRVN 540

Query: 541 CLS 544
            +S
Sbjct: 541 RVS 543

[Close]

BLAST of Lsi06G014970 vs. NCBI nr
Match: XP_022972528.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 973.8 bits (2516), Expect = 6.2e-280
Identity = 493/543 (90.79%), Postives = 517/543 (95.21%), Query Frame = 0


Query: 1   MEVSGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGAN 60
           MEV GFM NKW+ATA+GIWIQCICGASYTFSIYSSALKSTQ YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVAPPNPRRAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AG+ISGFLYSAV P + RR+  GPWVVHAAGAIQCFLGYIFIWAAVSG+IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPASRRRSSVGPWVVHAAGAIQCFLGYIFIWAAVSGLIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMKGYLGLSGALLIQVYNT CNGDPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMKGYLGLSGALLIQVYNTACNGDPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTL 240
           F+LMLAV PTLLSIMFMWFVRID+TES NEMKHLNSLSALAVIVAFYLMVVII NNTF+L
Sbjct: 181 FVLMLAVFPTLLSIMFMWFVRIDRTESRNEMKHLNSLSALAVIVAFYLMVVIIFNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLGIALNAQREDFRESSPSLFTEKSHVVNKPKSVDAEDSVEY 300
           SSWTRF TFSILL+LL APLGIA+NAQREDFR SSPS FTE++ V+NKPKS +AEDSVEY
Sbjct: 241 SSWTRFVTFSILLVLLVAPLGIAINAQREDFRMSSPSFFTEENPVLNKPKSANAEDSVEY 300

Query: 301 HELPGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP DE Q +A +NSR P+AMNVLEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPSDELQTMAAANSRAPRAMNVLEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAA +HGSCSGTHCFVVSFLVMA+VAFLG LVAAALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAEKHGSCSGTHCFVVSFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
            LS
Sbjct: 541 RLS 543

[Close]

BLAST of Lsi06G014970 vs. NCBI nr
Match: XP_038879631.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 973.0 bits (2514), Expect = 1.1e-279
Identity = 502/543 (92.45%), Postives = 520/543 (95.76%), Query Frame = 0


Query: 1   MEVSGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGAN 60
           ME+SGFMPNKWIATALGIWIQCICGASYTFSIYS ALKSTQAYDQSTLDTVSVFKDIGAN
Sbjct: 1   MEISGFMPNKWIATALGIWIQCICGASYTFSIYSPALKSTQAYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVAPPNPRRAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGIISGFLYSAV  P P R FAGPWVVHAAGAIQ FLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTTPAP-RPFAGPWVVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNF HYSGT+VGIMKGYLGLSGAL+IQVY TTCNGDPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFEHYSGTVVGIMKGYLGLSGALIIQVY-TTCNGDPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTL 240
           FLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTF+L
Sbjct: 181 FLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLGIALNAQREDFRESSPSLFTEKSHVVNKPKSVDAEDSVEY 300
           S+WTRFFTFSILLILLAAPLGIA+NAQ+EDFRE SPS  +EKS  VNK +S+DAEDSVEY
Sbjct: 241 SAWTRFFTFSILLILLAAPLGIAINAQKEDFREPSPSFISEKSQ-VNKSESMDAEDSVEY 300

Query: 301 HELPGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP +E+QI+AV+NSR PQ +NVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQ+GQS
Sbjct: 301 HELPREENQIMAVANSRTPQPLNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQIGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLL MSGGHI+IA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLGMSGGHIMIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQRRVE 540
           IGYIYDREA  EHGSC+GTHCFVVSFLVMAVVAF G LVAAALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREATEEHGSCNGTHCFVVSFLVMAVVAFFGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
           CLS
Sbjct: 541 CLS 540

[Close]

BLAST of Lsi06G014970 vs. NCBI nr
Match: XP_023531919.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 968.4 bits (2502), Expect = 2.6e-278
Identity = 491/543 (90.42%), Postives = 515/543 (94.84%), Query Frame = 0


Query: 1   MEVSGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGAN 60
           MEV GFM NKW+ATA+GIWIQCICGASYTFSIYSSALKSTQ YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVAPPNPRRAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AG+ISGFLYSAV P   RR+  GPWVVHAAGAIQCFLGYIFIWAAVSG+IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPAARRRSSLGPWVVHAAGAIQCFLGYIFIWAAVSGLIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMKGYLGLSGALLIQVYNT CNG+PSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMKGYLGLSGALLIQVYNTACNGEPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTL 240
           FLLMLAV PTLLSIMFMWFVRID+TES NEMKHLNSLSALAVIVAFY+MVVII NNTF+L
Sbjct: 181 FLLMLAVFPTLLSIMFMWFVRIDRTESRNEMKHLNSLSALAVIVAFYVMVVIIFNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLGIALNAQREDFRESSPSLFTEKSHVVNKPKSVDAEDSVEY 300
           SSWTRF TFSILL+LL APLGIA+NAQRED R SSPS FTE++ V+NKPKS +AEDSVEY
Sbjct: 241 SSWTRFVTFSILLVLLVAPLGIAINAQREDLRMSSPSFFTEENPVLNKPKSANAEDSVEY 300

Query: 301 HELPGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP DE Q +A +NSR P+AMNVLEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPSDELQTMAAANSRAPRAMNVLEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAA +HGSCSGTHCFVVSFLVMA+VAFLG LVAAALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAEKHGSCSGTHCFVVSFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
            LS
Sbjct: 541 RLS 543

[Close]

BLAST of Lsi06G014970 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 626.7 bits (1615), Expect = 1.7e-179
Identity = 322/536 (60.07%), Postives = 403/536 (75.19%), Query Frame = 0


Query: 10  KWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGANAGIISGFLY 69
           KW+A    IWIQC  GASYTF IYS+ LKSTQ+YDQSTLDTVSVFKDIGANAG+ SG LY
Sbjct: 7   KWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVFSGLLY 66

Query: 70  SAVAPPNPR------RAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCFFMF 129
           +       R          GPWVV A GAIQCF GY  IWA+V+G+I +PPVP MC FMF
Sbjct: 67  TYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLMCLFMF 126

Query: 130 LAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLL 189
           LAA +QTFFNTANVV+ V NFA Y GT VGIMKG+LGLSGA+LIQ+Y T C GDP++F+L
Sbjct: 127 LAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDPASFIL 186

Query: 190 MLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSW 249
           +LAV PT+LS++ M  VRI +T  +++ KHLN LSA+++I+A YLM++IIL NTF LSSW
Sbjct: 187 LLAVTPTVLSLLVMPLVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTFGLSSW 246

Query: 250 TRFFTFSILLILLAAPLGIALNAQREDFRESSPSLFTEKSHVVNKPKSVDAEDSVEYHEL 309
               T   LL++LA PL IA  AQR+   ++ P    + S +++ PK+  + +       
Sbjct: 247 ANIVTLVCLLVMLALPLLIARRAQRDGMEKTVPH---DYSPLISSPKATTSGN----QSS 306

Query: 310 PGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGY 369
            GD      +S +     +N+L+A++ ++FWLLFLAM+CGMGSGL+TINN+ Q+G+SL Y
Sbjct: 307 EGDSKVEAGLSEN-----LNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRY 366

Query: 370 TATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGF 429
           ++ E  + VSLWSIWNFLGRFGAGY SD LLH  GW RPLLMA TL  MS GH++IASGF
Sbjct: 367 SSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGF 426

Query: 430 SGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGY 489
            GNLYVGS++VG+CYGSQWSLMPTITSE+FG+ HMGTIFNTI++ASP+GSYIFSVR+IGY
Sbjct: 427 QGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGY 486

Query: 490 IYDREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQRRV 540
           IYD+ A+GE  +C G+HCF +SF++MA VAF GFLVA  LFFRT+  YR  L +R+
Sbjct: 487 IYDKTASGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYRQILVKRL 530

[Close]

BLAST of Lsi06G014970 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 578.9 bits (1491), Expect = 4.1e-165
Identity = 297/532 (55.83%), Postives = 390/532 (73.31%), Query Frame = 0


Query: 10  KWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGANAGIISGFLY 69
           KW+A    IWIQC  G SYTF IYS+ LKSTQ+YDQSTLDTVSVFKDIG N G++SG +Y
Sbjct: 9   KWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVLSGLVY 68

Query: 70  SAVAPPNPRR----AFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCFFMFLA 129
           +A      RR       GPWVV   GAI  F GY  +WA+V+G+I RPPVP MC FMF+A
Sbjct: 69  TAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCLFMFIA 128

Query: 130 AHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLLML 189
           A + TF NTANVV+ + NFA Y GT VGIMKG++GLSGA+LIQ+Y   C GDP  F+L+L
Sbjct: 129 AQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKTFILLL 188

Query: 190 AVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTR 249
           A++P+LLS++ M  VR+ KT + +E KHL+ LS L++I+A YLM+ IIL +T +L SW  
Sbjct: 189 AIVPSLLSVLVMPLVRVYKTSTVDEKKHLDGLSTLSLIIAAYLMITIILKSTLSLPSWAN 248

Query: 250 FFTFSILLILLAAPLGIALNAQREDFRESSPSLFTEKSHVVNKPKSVDAEDSVEYHELPG 309
             T ++LL+LL++PL +A+ A R+   +   S++   S +V+   +++A  S E   L  
Sbjct: 249 AVTLAVLLVLLSSPLLVAVRAHRDSIEKPLSSVY---SPLVD---NLEATTSGEILMLDE 308

Query: 310 DESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTA 369
           D+S             +N+L+A+  ++FWLLFLAM+CGMGSG++TINN+ Q+G+SL YT+
Sbjct: 309 DKS-------------LNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGESLRYTS 368

Query: 370 TETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSG 429
            E  + ++LW+IWNF+GRFG GY SD+LLH  GW RPLLMA TL  M+ GH++IASGF G
Sbjct: 369 VEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLIIASGFQG 428

Query: 430 NLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIY 489
           NLY GSI+VGICYGSQWSLMPTITSE+FG++HMGTI+NTI+IASP+GSYIFSVR+IGYIY
Sbjct: 429 NLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSVRLIGYIY 488

Query: 490 DREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQR 538
           DR   GE  +C G HCF ++++V+A VAFLGFLV+  L FRT+  YR   ++
Sbjct: 489 DRTIIGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIYRQIFEK 521

[Close]

BLAST of Lsi06G014970 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 574.7 bits (1480), Expect = 7.8e-164
Identity = 304/538 (56.51%), Postives = 383/538 (71.19%), Query Frame = 0


Query: 6   FMPNKWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGANAGIIS 65
           F   KW+A A  IWIQ   GASYTF IYSS LKS+Q+YDQSTLDTVSV+KDIGAN GI+S
Sbjct: 3   FANTKWVAAAASIWIQSFSGASYTFGIYSSVLKSSQSYDQSTLDTVSVYKDIGANVGILS 62

Query: 66  GFLYSAVAPPNPRRA--FAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCFFMF 125
           G  Y+AVA         F+GPW+V   G +Q F+GY FIW A SGVI RPPV  MC FMF
Sbjct: 63  GLFYTAVASRKSGNGGFFSGPWLVIFVGLLQWFVGYGFIWMATSGVIPRPPVAMMCLFMF 122

Query: 126 LAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLL 185
            A H Q FFNTA VVT V NF+ Y GT VGIMKGYLGLSGA+L+Q+Y+  C GDP N++L
Sbjct: 123 FAGHCQPFFNTAIVVTAVRNFSDYGGTAVGIMKGYLGLSGAILVQMYHIFCGGDPRNYIL 182

Query: 186 MLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSW 245
           +LAV+P+LL +  M FVR   T  + + KHLN LSA+++I+  YLMVVI++ N   +S  
Sbjct: 183 LLAVVPSLLILTLMPFVRTYDTVIAGDKKHLNGLSAISLIIVTYLMVVILVENIIGMSMP 242

Query: 246 TRFFTFSILLILLAAPLGIALNAQREDFRE--SSPSLFTEKSHVVNKPKSVDAEDSVEYH 305
            +  +F+ LL+LLA+PL +A+ AQRE+     S     TE++ +++ PK   + D  +  
Sbjct: 243 MKICSFTFLLLLLASPLLVAVRAQREEEHRFLSLDFPVTERTTLLDSPKLNSSSDVKDV- 302

Query: 306 ELPGDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSL 365
                           +   MNVLEAI T NFWLLF+AM+CGMGSGLATINN+ Q+G+SL
Sbjct: 303 ----------------MTNDMNVLEAICTTNFWLLFVAMICGMGSGLATINNIRQMGESL 362

Query: 366 GYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIAS 425
            Y+  +  + VSLWSIWNFLGRFG+GY SD  LHS+GW RP+ MAITL +M+ GHIV+AS
Sbjct: 363 RYSTVQLNSLVSLWSIWNFLGRFGSGYISDTYLHSHGWPRPVFMAITLGLMAIGHIVMAS 422

Query: 426 GFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVI 485
           G  G+LY+GS+LVG+ YGSQWSLMPTITSEIFG+ HMGTIF TI+IASP+GSY FSV+VI
Sbjct: 423 GLLGSLYIGSLLVGLAYGSQWSLMPTITSEIFGVLHMGTIFYTISIASPVGSYFFSVKVI 482

Query: 486 GYIYDREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQRRV 540
           GY+YD+ A+ +  SC G HCF  SFL+MA +A LG LVA  L  RT++FY   + +R+
Sbjct: 483 GYLYDKVASEDDHSCYGNHCFRTSFLIMAAMALLGSLVALVLLLRTKKFYATLVAKRI 523

[Close]

BLAST of Lsi06G014970 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 557.0 bits (1434), Expect = 1.7e-158
Identity = 290/534 (54.31%), Postives = 384/534 (71.91%), Query Frame = 0


Query: 10  KWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGANAGIISGFLY 69
           KW+A A  IWIQ   GA+YTF+IYSS LKS+Q+YDQSTLD VSVFKDIG   GIISGFLY
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 70  SAVAPPNPRRAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCFFMFLAAHAQ 129
           +A+   +  R F GPWVV   G +Q F+G+ FIWA+V G+I  PPVP MC F+FLA H+ 
Sbjct: 67  TAMT--SKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSL 126

Query: 130 TFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGD--PSNFLLMLAV 189
            FFNTANVVT   NF+ Y GT VGIM+G+LGLSGA+LIQ+Y+  C G+  P+ F+L+LA+
Sbjct: 127 PFFNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAI 186

Query: 190 LPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFF 249
           +PTL+  + M FVR+ +T + ++ KHL+ LSA+++I+A YLMVVI + N   LS   + F
Sbjct: 187 VPTLVMFLAMPFVRVYETVTISDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQIF 246

Query: 250 TFSILLILLAAPLGIALNAQREDFRESSPSL---FTEKSHVVNKPKSVDAEDSVEYHELP 309
           +F ++L+LLA+PL +A+ A RE  R++  SL     + S +++ P               
Sbjct: 247 SFILVLLLLASPLLVAVRALREK-RQTLSSLDGPVLDTSALLDPP--------------- 306

Query: 310 GDESQIVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYT 369
              S I    +  + +  N+LEA+ T+NFWLLFLAM+CGMGSG AT+NNM Q+G+SL Y+
Sbjct: 307 --SSNIFPDGDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYS 366

Query: 370 ATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFS 429
           + +  + VSLWSIWNFLGRFGAGY SD  LH + W RP+ MAITL +M+ GHI++ASG  
Sbjct: 367 SVQLNSLVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQ 426

Query: 430 GNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYI 489
           G+LY GS+L+G+ YGSQWSLMPTITSEIFG+ HMGTI+ TI+IA P+GSYI SV+VIGY 
Sbjct: 427 GSLYAGSVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYF 486

Query: 490 YDREAAGEHGSCSGTHCFVVSFLVMAVVAFLGFLVAAALFFRTRRFYRLALQRR 539
           YD+ A+ +  SC G+ CF  SF++MA VA  G LVA+ LFFRT +FY+  + +R
Sbjct: 487 YDKVASEDDNSCFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYKNLVAKR 520

[Close]

BLAST of Lsi06G014970 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 341.3 bits (874), Expect = 1.5e-93
Identity = 202/560 (36.07%), Postives = 322/560 (57.50%), Query Frame = 0


Query: 7   MPNKWIATALGIWIQCICGASYTFSIYSSALKSTQAYDQSTLDTVSVFKDIGANAGIISG 66
           +  +W      + I    GA+Y F IYS  +K T  YDQ+TL+ +S FKD+GAN G+++G
Sbjct: 18  LTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVLAG 77

Query: 67  FLYSAVAPPNPRRAFAGPWVVHAAGAIQCFLGYIFIWAAVSGVIDRPPVPAMCFFMFLAA 126
            L + V P         PW +   GAI  F GY  IW AV+  I +P V  MC ++ + A
Sbjct: 78  LL-NEVTP---------PWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGA 137

Query: 127 HAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLLMLA 186
           ++Q+F NT ++VT V NF    G ++GI+KGY+GLSGA++ Q+Y      D    +LM+ 
Sbjct: 138 NSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIG 197

Query: 187 VLPTLLSIMFMWFVRIDKTE-SSNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTR 246
            LP ++S  F+  +RI K +  +NE+K   +   +++ +A +LMVVII+N     +    
Sbjct: 198 WLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEF 257

Query: 247 FFTFSILLILLAAPLGIALNAQREDFRESSPSLFTEK--SHVVNKPKSVDAEDSVEYHEL 306
             + +++++LL  P+ + +  +++ ++E   +L      + V  KPK     DS E+ + 
Sbjct: 258 GGSAAVVIVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKL----DSSEFKDD 317

Query: 307 PGDESQIVAVSNSRIPQA-------------MNVLEAIRTINFWLLFLAMVCGMGSGLAT 366
            G+ES+ V V   + P                 +L+A+ +++  +LFLA +CG+G  L  
Sbjct: 318 DGEESKEV-VEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTA 377

Query: 367 INNMSQLGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLL 426
           I+N+ Q+G SLGY      TFVSL SIWN+ GR  +G  S+  L  Y + RPL++ + LL
Sbjct: 378 IDNLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLL 437

Query: 427 IMSGGHIVIASGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASP 486
           +   GH++IA    G LYV S+++G C+G+QW L+  I SEIFGL++  T++N  ++ASP
Sbjct: 438 LSCAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASP 497

Query: 487 LGSYIFSVRVIGYIYDREAA------------GEHGSCSGTHCFVVSFLVMAVVAFLGFL 539
           +GSY+ +VRV GY+YD EA             G+  +C GT CF +SF+++A V   G L
Sbjct: 498 IGSYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVL 557

The following BLAST results are available for this feature:

BLAST of Lsi06G014970 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	5.5e-29	25.71	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]
Q6CGU8	1.5e-10	28.16	Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28...	[more]
Q96TW9	5.3e-08	27.03	Probable transporter MCH1 OS=Wickerhamomyces anomalus OX=4927 GN=MCH1 PE=3 SV=1	[more]
Q6FWD4	1.2e-07	24.64	Probable transporter MCH1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM ...	[more]

BLAST of Lsi06G014970 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UT75	7.4e-287	93.19	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BWC2	7.4e-287	93.19	protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 ...	[more]
A0A0A0LUE8	7.9e-281	91.53	Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G00847...	[more]
A0A6J1IA87	3.0e-280	90.79	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471...	[more]
A0A6J1F604	1.1e-277	90.24	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of Lsi06G014970 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008453166.1	1.5e-286	93.19	PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo] >KAA0057917.1 prote...	[more]
XP_011659929.1	1.6e-280	91.53	protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN63641.1 hypothetical pr...	[more]
XP_022972528.1	6.2e-280	90.79	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima]	[more]
XP_038879631.1	1.1e-279	92.45	protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida]	[more]
XP_023531919.1	2.6e-278	90.42	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi06G014970 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74780.1	1.7e-179	60.07	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT1G18940.1	4.1e-165	55.83	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT2G34350.1	7.8e-164	56.51	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT2G34355.1	1.7e-158	54.31	Major facilitator superfamily protein	[more]
AT2G39210.1	1.5e-93	36.07	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011701	Major facilitator superfamily	PFAM	PF07690	MFS_1	coord: 335..528 e-value: 8.2E-10 score: 38.1
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 10..263 e-value: 4.1E-65 score: 219.7
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 213..525 e-value: 2.0E-11 score: 45.2
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 216..529
None	No IPR available	PANTHER	PTHR21576:SF22	F25A4.25 PROTEIN	coord: 9..540
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 9..540
None	No IPR available	CDD	cd17354	MFS_Mch1p_like	coord: 10..516 e-value: 8.65897E-118 score: 351.169

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G014970.1	Lsi06G014970.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0022857	transmembrane transporter activity