Lsi06G014430 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G014430
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionHNH domain-containing protein
Locationchr06: 24963033 .. 24965771 (+)
RNA-Seq ExpressionLsi06G014430
SyntenyLsi06G014430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTAACAAGTAGGAAATGATCTCACGTCTTCAGTTTCTTCCCTTTCTGTCTTCTACAAGTCCGATCCGCTGGCCGGCGTCCCGACTCCGATGGAAACTACTTCCAGTTCGCCGTCACCGACTCGTTCCCGCGGCGGACATAGACACAGTGGCTCCGGAAGTGGGGACGAACGGCCAAGATTTTTCGACGCAAAAGCGAAAGCCATTTGTTGGGCTAAGGCGGATGTTGTCCCTGGCCGACATCCCGAGCGTTGGCGTAAGGACGCCGCTGGTAATGTCGTCTGTAAGCGCTTCTGCAACTGCCAGGGCTGCCTCTGCTTCGAGTACGATCACATTGTCCCTTACTCCAAAGGTAATATGATTTCCATCTCTGACACCTTTAGTCGACGAATGGTTTCTGATTCCTATTCGAGAATTTTTTATTCTGCTGTTGCGTTTTCGTAGTGGATGACTGGATATATTTAAATTTAGGGCTTAGCTCTGATTGTGATGAAATTCGATCGGCGATTGTTTGCAGGCGGCGAATCCACAGCGGATAATTGCCAGATTCTTCAGACTAGAGTAAACAGATTCAAATCGGACAAAAACGACGTTGATACGTCTGAGTTGAAAGGCTATTCATGTGATGTCAAGTTTACTGGTATTTTTTCATCTGCTCCAGTTCTTTCTTTTGGAATGATCATGCTCTACTGTTTAGTTTTATAACACTCGTTAGGGCTCGAGAGTCGAGACTATAAAATGGTAAACCTATGTTTGATGTCTGAATTTTCATATCCCAAATCATGGGCTGCTAATCTATGACCTGTCCATACCAAGTTACATAAAATTAAACTTTTAAAATGATCTCACCTTCTATACTGCCTATTAATTTTCATAAATCCTTTCTGCCGTACTATTCAAACGCAAGAACTTATCAAATTCGCGCTTGTTAAAGCTACGAGTACAACATCCGCTTGGAATACCCAAGTAGTTAACCTTTCTGTTGTTTTGTTCCTTCTTGCAATCTCTCCCAGTAGAATATAGCTTTTAGCATCAATTTCTTTTTCTCTTAGCTGCCCCATATGGCCATATGTTCATCAGCTTTTACATAATCATATGGTTTTTTGCTCAAAATTTGTAATTTCTGTAAATTAAAAACTCAGCCATTCCATGAGAATTGATTGGCTTGACTGTTGTGCTGTTGTTTAACTTTTGACAGTACTTGGTGACATTTTTGGGGGGAGTGGTCTAGAAACATCATTACAGTTTCAGGAAAGTGCTAGAAAGTGAAGTTTCAATTGTTTTAGAAATATCTGTTGTCCTGTTCCATATTAGCTGTCATTGCTGGTGGTAGTTTGTGATTAAAAATTTAAAACAAACGAATACTTGTATTTTAGGTCATCTGAAACACAAATGAAGAATCTCCATAGATTGCTGTCTTTAACTTCGAACTATAAATTCACCTCCTTAATGTTATGAATTCAAATCATGCTGTGTACCCACGTACAATATCAAATATCTTATGAAATTTTAATCATTAAATGATGTAGAGAAATTAAATTGTTATGCATAAGTGGAATAGAGCACGCTCTGTGCATCTTGATCAATACCTAAAACTGGAGAAAGTCCTCTGGGTAGGAATTCTTCAAGGAACACATGGATTACCTCTTAGTTCATTGTCAATTTCCATATTCGGGTTTCAATGAGACATTGAGATCAACTTGCATGTATCTCAACGGATCTCACAAGATAATTACTTTATCTTACTACACGAGAAAAAAAAATGAAGGAATACAAGGGTGTACAGAAAAACCAAGCCCACAAAGCCCCCTTTAAAGAGAAAGGGGTCAAATTAAGAAAAATGTTGCCTAAAGAGTAATTACAAAAGAACTTCGAAAGCGAAGTTCAAAGGGAGACAAGAAACTTCACCAAGGACCAAACATCACTAGAGTCCCTCTCCACCCCTTTAAACACTACTATTTCTCTCTCCATAAATCTCATAACAAAGCACACACCCAACATTCTAAAGAAAGCAGTCATTCTCACTAAAAGAGGCGGATGGAGGAGGTACTCTCCGATCAAACTACTGACACTGCTCTTGGATCTCAAGTGGATGTCCACCATGAATTGAACTCATTGTGCTTAACCTTTCAAACGTGCCCTAGTATCCACTAGACCATCTCATGGTAGTTAAGGTTTTGAAGTCGATGAAAACAATTGACTCTCCCTTCGAGGTTCTTTTCCCCACTCTGTCTTACATCTATATATTTATGATGTATTTGGAGTGCATATTTCGTTGCAGATAAGGAGCTGGACATAATTGAAATGGCTGTTTATGGAGATGTAATCCGGCCAGGGAACCAGTGTCGTTGCAGAACAGTTTCTGAAATGCTTGGCCAATATAAATCCAAAGACCGCTTGGCCCCCTGCAAATTGCCGTATAATGAAGATTCTTTGTAACAACAAAGAAGACTTAAAACTAAAGCATGTTTGCCCCATTTGGTATAGATCTAGAATTTAATACGCTCGATACGAATACTGTTAGATTATATCAATTAGAAACTGTGGACCTCCATTACTTGGTTAGATCAATATCAATGAACATCGACCATCATTTCTTTATGTATTATTAATACAATGTTGTGTGTTCATTAAATAGTTGAAAAGAAAGTTTAAAGACACAAAATTCAAATATGTGGCAAACAGATTTTGGACTTTAAAAATAAGATCTACTAGACACTTTCTAAAAATTTACAAAA

mRNA sequence

AAATTAACAAGTAGGAAATGATCTCACGTCTTCAGTTTCTTCCCTTTCTGTCTTCTACAAGTCCGATCCGCTGGCCGGCGTCCCGACTCCGATGGAAACTACTTCCAGTTCGCCGTCACCGACTCGTTCCCGCGGCGGACATAGACACAGTGGCTCCGGAAGTGGGGACGAACGGCCAAGATTTTTCGACGCAAAAGCGAAAGCCATTTGTTGGGCTAAGGCGGATGTTGTCCCTGGCCGACATCCCGAGCGTTGGCGTAAGGACGCCGCTGGTAATGTCGTCTGTAAGCGCTTCTGCAACTGCCAGGGCTGCCTCTGCTTCGAGTACGATCACATTGTCCCTTACTCCAAAGGCGGCGAATCCACAGCGGATAATTGCCAGATTCTTCAGACTAGAGTAAACAGATTCAAATCGGACAAAAACGACGTTGATACGTCTGAGTTGAAAGGCTATTCATGTGATGTCAAGTTTACTGATAAGGAGCTGGACATAATTGAAATGGCTGTTTATGGAGATGTAATCCGGCCAGGGAACCAGTGTCGTTGCAGAACAGTTTCTGAAATGCTTGGCCAATATAAATCCAAAGACCGCTTGGCCCCCTGCAAATTGCCGTATAATGAAGATTCTTTGTAACAACAAAGAAGACTTAAAACTAAAGCATGTTTGCCCCATTTGGTATAGATCTAGAATTTAATACGCTCGATACGAATACTGTTAGATTATATCAATTAGAAACTGTGGACCTCCATTACTTGGTTAGATCAATATCAATGAACATCGACCATCATTTCTTTATGTATTATTAATACAATGTTGTGTGTTCATTAAATAGTTGAAAAGAAAGTTTAAAGACACAAAATTCAAATATGTGGCAAACAGATTTTGGACTTTAAAAATAAGATCTACTAGACACTTTCTAAAAATTTACAAAA

Coding sequence (CDS)

ATGGAAACTACTTCCAGTTCGCCGTCACCGACTCGTTCCCGCGGCGGACATAGACACAGTGGCTCCGGAAGTGGGGACGAACGGCCAAGATTTTTCGACGCAAAAGCGAAAGCCATTTGTTGGGCTAAGGCGGATGTTGTCCCTGGCCGACATCCCGAGCGTTGGCGTAAGGACGCCGCTGGTAATGTCGTCTGTAAGCGCTTCTGCAACTGCCAGGGCTGCCTCTGCTTCGAGTACGATCACATTGTCCCTTACTCCAAAGGCGGCGAATCCACAGCGGATAATTGCCAGATTCTTCAGACTAGAGTAAACAGATTCAAATCGGACAAAAACGACGTTGATACGTCTGAGTTGAAAGGCTATTCATGTGATGTCAAGTTTACTGATAAGGAGCTGGACATAATTGAAATGGCTGTTTATGGAGATGTAATCCGGCCAGGGAACCAGTGTCGTTGCAGAACAGTTTCTGAAATGCTTGGCCAATATAAATCCAAAGACCGCTTGGCCCCCTGCAAATTGCCGTATAATGAAGATTCTTTGTAA

Protein sequence

METTSSSPSPTRSRGGHRHSGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSDKNDVDTSELKGYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNEDSL
Homology
BLAST of Lsi06G014430 vs. ExPASy TrEMBL
Match: A0A5A7UWI1 (HNH endonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002690 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 6.6e-99
Identity = 174/180 (96.67%), Postives = 176/180 (97.78%), Query Frame = 0

Query: 1   METTSSSPSPTRSRGGHRHSGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAA 60
           ME TSSSPSPTRSRGG R SGSGSGDERPRFFDAKAKA CWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSDKNDVDTSELKG 120
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKS+K+DVDTSELKG
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNEDSL 180
           YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNEDSL
Sbjct: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNEDSL 180

BLAST of Lsi06G014430 vs. ExPASy TrEMBL
Match: A0A1S3BVP6 (uncharacterized protein LOC103494011 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494011 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 6.6e-99
Identity = 174/180 (96.67%), Postives = 176/180 (97.78%), Query Frame = 0

Query: 1   METTSSSPSPTRSRGGHRHSGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAA 60
           ME TSSSPSPTRSRGG R SGSGSGDERPRFFDAKAKA CWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSDKNDVDTSELKG 120
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKS+K+DVDTSELKG
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNEDSL 180
           YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNEDSL
Sbjct: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNEDSL 180

BLAST of Lsi06G014430 vs. ExPASy TrEMBL
Match: A0A1S3BVR0 (uncharacterized protein LOC103494011 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494011 PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 3.6e-97
Identity = 174/184 (94.57%), Postives = 176/184 (95.65%), Query Frame = 0

Query: 1   METTSSSPSPTRSRGGHRHSGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAA 60
           ME TSSSPSPTRSRGG R SGSGSGDERPRFFDAKAKA CWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSDKNDVDTSELKG 120
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKS+K+DVDTSELKG
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 121 YSCDVKFT----DKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYN 180
           YSCDVKFT    DKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYN
Sbjct: 121 YSCDVKFTGFLADKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYN 180

BLAST of Lsi06G014430 vs. ExPASy TrEMBL
Match: A0A0A0LUK3 (HNHc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G011480 PE=4 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 4.7e-97
Identity = 170/178 (95.51%), Postives = 174/178 (97.75%), Query Frame = 0

Query: 1   METTSSSPSPTRSRGGHRHSGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAA 60
           ME TSSSPSPTRSRGG R SGSGSGDERPRFFDAKAKA CWAKADVVPGRHPERWRKDAA
Sbjct: 30  MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 89

Query: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSDKNDVDTSELKG 120
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKS+K+DVDTSELKG
Sbjct: 90  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 149

Query: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNED 179
           YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSE+LGQYKSKDRLAPCKLPYNE+
Sbjct: 150 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEE 207

BLAST of Lsi06G014430 vs. ExPASy TrEMBL
Match: A0A6J1C0N7 (uncharacterized protein LOC111007267 OS=Momordica charantia OX=3673 GN=LOC111007267 PE=4 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 4.7e-97
Identity = 167/180 (92.78%), Postives = 176/180 (97.78%), Query Frame = 0

Query: 1   METTSSSPSPTRSRGGHRHSGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAA 60
           METTS SPSPTRSRGGHRHSGSG+GDERPRFFD KAKAICWAKAD+VPGRHP+RWRKDAA
Sbjct: 1   METTSRSPSPTRSRGGHRHSGSGNGDERPRFFDTKAKAICWAKADIVPGRHPDRWRKDAA 60

Query: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSDKNDVDTSELKG 120
           GNVVCKRFCNCQGCLCFEYDHIVP+SKGGESTA+NCQILQTRVNRFKSDK+DVD S+LKG
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPFSKGGESTAENCQILQTRVNRFKSDKDDVDASQLKG 120

Query: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNEDSL 180
           YSCDV+FTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLP +EDSL
Sbjct: 121 YSCDVRFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPSSEDSL 180

BLAST of Lsi06G014430 vs. NCBI nr
Match: XP_038878253.1 (uncharacterized protein LOC120070538 [Benincasa hispida])

HSP 1 Score: 374.0 bits (959), Expect = 7.2e-100
Identity = 173/180 (96.11%), Postives = 177/180 (98.33%), Query Frame = 0

Query: 1   METTSSSPSPTRSRGGHRHSGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAA 60
           ME TSSSPSPTRSRGGHRHSGSGSGDERPRFFD KAKAICW KADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGHRHSGSGSGDERPRFFDTKAKAICWDKADVVPGRHPERWRKDAA 60

Query: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSDKNDVDTSELKG 120
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKS++NDVDTSELKG
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNRNDVDTSELKG 120

Query: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNEDSL 180
           YSCD+KFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYK+KDRLAPCKLPYNEDSL
Sbjct: 121 YSCDIKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKAKDRLAPCKLPYNEDSL 180

BLAST of Lsi06G014430 vs. NCBI nr
Match: XP_008453224.1 (PREDICTED: uncharacterized protein LOC103494011 isoform X2 [Cucumis melo] >KAA0057965.1 HNH endonuclease [Cucumis melo var. makuwa])

HSP 1 Score: 369.8 bits (948), Expect = 1.4e-98
Identity = 174/180 (96.67%), Postives = 176/180 (97.78%), Query Frame = 0

Query: 1   METTSSSPSPTRSRGGHRHSGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAA 60
           ME TSSSPSPTRSRGG R SGSGSGDERPRFFDAKAKA CWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSDKNDVDTSELKG 120
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKS+K+DVDTSELKG
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNEDSL 180
           YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNEDSL
Sbjct: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNEDSL 180

BLAST of Lsi06G014430 vs. NCBI nr
Match: XP_008453223.1 (PREDICTED: uncharacterized protein LOC103494011 isoform X1 [Cucumis melo])

HSP 1 Score: 364.0 bits (933), Expect = 7.5e-97
Identity = 174/184 (94.57%), Postives = 176/184 (95.65%), Query Frame = 0

Query: 1   METTSSSPSPTRSRGGHRHSGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAA 60
           ME TSSSPSPTRSRGG R SGSGSGDERPRFFDAKAKA CWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSDKNDVDTSELKG 120
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKS+K+DVDTSELKG
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 121 YSCDVKFT----DKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYN 180
           YSCDVKFT    DKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYN
Sbjct: 121 YSCDVKFTGFLADKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYN 180

BLAST of Lsi06G014430 vs. NCBI nr
Match: XP_004138181.1 (uncharacterized protein LOC101204599 [Cucumis sativus] >KAE8652425.1 hypothetical protein Csa_014117 [Cucumis sativus])

HSP 1 Score: 363.6 bits (932), Expect = 9.7e-97
Identity = 170/178 (95.51%), Postives = 174/178 (97.75%), Query Frame = 0

Query: 1   METTSSSPSPTRSRGGHRHSGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAA 60
           ME TSSSPSPTRSRGG R SGSGSGDERPRFFDAKAKA CWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSDKNDVDTSELKG 120
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKS+K+DVDTSELKG
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNED 179
           YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSE+LGQYKSKDRLAPCKLPYNE+
Sbjct: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEE 178

BLAST of Lsi06G014430 vs. NCBI nr
Match: XP_022135260.1 (uncharacterized protein LOC111007267 [Momordica charantia])

HSP 1 Score: 363.6 bits (932), Expect = 9.7e-97
Identity = 167/180 (92.78%), Postives = 176/180 (97.78%), Query Frame = 0

Query: 1   METTSSSPSPTRSRGGHRHSGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAA 60
           METTS SPSPTRSRGGHRHSGSG+GDERPRFFD KAKAICWAKAD+VPGRHP+RWRKDAA
Sbjct: 1   METTSRSPSPTRSRGGHRHSGSGNGDERPRFFDTKAKAICWAKADIVPGRHPDRWRKDAA 60

Query: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSDKNDVDTSELKG 120
           GNVVCKRFCNCQGCLCFEYDHIVP+SKGGESTA+NCQILQTRVNRFKSDK+DVD S+LKG
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPFSKGGESTAENCQILQTRVNRFKSDKDDVDASQLKG 120

Query: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNEDSL 180
           YSCDV+FTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLP +EDSL
Sbjct: 121 YSCDVRFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPSSEDSL 180

BLAST of Lsi06G014430 vs. TAIR 10
Match: AT1G18680.1 (HNH endonuclease domain-containing protein )

HSP 1 Score: 262.7 bits (670), Expect = 2.2e-70
Identity = 119/172 (69.19%), Postives = 140/172 (81.40%), Query Frame = 0

Query: 3   TTSSSPSPTRSRGGHRHSGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAAGN 62
           T ++ P P+      R +   +G+ERPRFFD KAK  CWA AD+VPGRHPERWRKD AGN
Sbjct: 14  TAAAVPPPSPPSSPSRSNVKSNGEERPRFFDGKAKNKCWANADIVPGRHPERWRKDVAGN 73

Query: 63  VVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSDKNDVDTSELKGYS 122
           +VCKRF NC GCLCFEYDHIVPYSKGGES A+NCQILQTRVNRFKS + +VD + LK YS
Sbjct: 74  IVCKRFGNCNGCLCFEYDHIVPYSKGGESIAENCQILQTRVNRFKSAQENVDATTLKSYS 133

Query: 123 CDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLP 175
           C ++FTDKELD+IEMAVYGDV+RPG +CRC+TV+E+LGQ KSKD  A C+LP
Sbjct: 134 CGLQFTDKELDVIEMAVYGDVLRPGKECRCKTVAELLGQSKSKDGKAACELP 185

BLAST of Lsi06G014430 vs. TAIR 10
Match: AT3G47490.1 (HNH endonuclease )

HSP 1 Score: 144.4 bits (363), Expect = 8.6e-35
Identity = 64/134 (47.76%), Postives = 85/134 (63.43%), Query Frame = 0

Query: 20  SGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEY 79
           SG G  +  PR F    K  CW KA+ + GR PERWR+D  GN+V ++   C GCLC +Y
Sbjct: 99  SGYGDPNPEPRSFPYSVKQQCWEKAEKIKGRDPERWRRDHLGNIVFRKLVGCPGCLCHDY 158

Query: 80  DHIVPYSKGGESTADNCQILQTRVNRFKSDKNDVDTSELKGYSCDVKFTDKELDIIEMAV 139
           DHIVPYSKGG+ST +NCQ+LQ +VNR K +K D+  SEL   S   +   +++D+IE+  
Sbjct: 159 DHIVPYSKGGKSTLENCQVLQAKVNRSKGNKTDISRSELIQRSSYCRVAGRDMDLIELTA 218

Query: 140 YGDVIRPGNQCRCR 154
           YG+V R      CR
Sbjct: 219 YGNVQRAPTSSGCR 232

BLAST of Lsi06G014430 vs. TAIR 10
Match: AT3G47490.3 (HNH endonuclease )

HSP 1 Score: 132.5 bits (332), Expect = 3.4e-31
Identity = 64/154 (41.56%), Postives = 85/154 (55.19%), Query Frame = 0

Query: 20  SGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEY 79
           SG G  +  PR F    K  CW KA+ + GR PERWR+D  GN+V ++   C GCLC +Y
Sbjct: 60  SGYGDPNPEPRSFPYSVKQQCWEKAEKIKGRDPERWRRDHLGNIVFRKLVGCPGCLCHDY 119

Query: 80  DHIVPYS--------------------KGGESTADNCQILQTRVNRFKSDKNDVDTSELK 139
           DHIVPYS                    KGG+ST +NCQ+LQ +VNR K +K D+  SEL 
Sbjct: 120 DHIVPYSKDMCPLEHAFDEPHKDFLDLKGGKSTLENCQVLQAKVNRSKGNKTDISRSELI 179

Query: 140 GYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCR 154
             S   +   +++D+IE+  YG+V R      CR
Sbjct: 180 QRSSYCRVAGRDMDLIELTAYGNVQRAPTSSGCR 213

BLAST of Lsi06G014430 vs. TAIR 10
Match: AT3G47490.2 (HNH endonuclease )

HSP 1 Score: 87.4 bits (215), Expect = 1.3e-17
Identity = 36/68 (52.94%), Postives = 44/68 (64.71%), Query Frame = 0

Query: 20  SGSGSGDERPRFFDAKAKAICWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEY 79
           SG G  +  PR F    K  CW KA+ + GR PERWR+D  GN+V ++   C GCLC +Y
Sbjct: 99  SGYGDPNPEPRSFPYSVKQQCWEKAEKIKGRDPERWRRDHLGNIVFRKLVGCPGCLCHDY 158

Query: 80  DHIVPYSK 88
           DHIVPYSK
Sbjct: 159 DHIVPYSK 166

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7UWI16.6e-9996.67HNH endonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002... [more]
A0A1S3BVP66.6e-9996.67uncharacterized protein LOC103494011 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A1S3BVR03.6e-9794.57uncharacterized protein LOC103494011 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0LUK34.7e-9795.51HNHc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G011480 PE=4 S... [more]
A0A6J1C0N74.7e-9792.78uncharacterized protein LOC111007267 OS=Momordica charantia OX=3673 GN=LOC111007... [more]
Match NameE-valueIdentityDescription
XP_038878253.17.2e-10096.11uncharacterized protein LOC120070538 [Benincasa hispida][more]
XP_008453224.11.4e-9896.67PREDICTED: uncharacterized protein LOC103494011 isoform X2 [Cucumis melo] >KAA00... [more]
XP_008453223.17.5e-9794.57PREDICTED: uncharacterized protein LOC103494011 isoform X1 [Cucumis melo][more]
XP_004138181.19.7e-9795.51uncharacterized protein LOC101204599 [Cucumis sativus] >KAE8652425.1 hypothetica... [more]
XP_022135260.19.7e-9792.78uncharacterized protein LOC111007267 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G18680.12.2e-7069.19HNH endonuclease domain-containing protein [more]
AT3G47490.18.6e-3547.76HNH endonuclease [more]
AT3G47490.33.4e-3141.56HNH endonuclease [more]
AT3G47490.21.3e-1752.94HNH endonuclease [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003615HNH nucleaseSMARTSM00507HNH_5coord: 51..105
e-value: 0.0083
score: 25.3
IPR003615HNH nucleaseCDDcd00085HNHccoord: 66..105
e-value: 2.96364E-5
score: 38.2216
IPR002711HNH endonucleasePFAMPF01844HNHcoord: 65..99
e-value: 3.5E-5
score: 23.9
NoneNo IPR availableGENE3D1.10.30.50coord: 48..129
e-value: 1.4E-8
score: 36.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availablePANTHERPTHR33427:SF1F6A14.21 PROTEINcoord: 17..178
NoneNo IPR availablePANTHERPTHR33427HNH ENDONUCLEASEcoord: 17..178

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G014430.1Lsi06G014430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0003676 nucleic acid binding