Lsi06G014310 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G014310
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	SNAP25 homologous protein SNAP33
Location	chr06: 24849521 .. 24852045 (+)
RNA-Seq Expression	Lsi06G014310
Synteny	Lsi06G014310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCACCTTTTAGGAAATCCCCTATAAATGTTGCTAGGCACGGCACAAGCCAACCTGCTAATCCTGCTCGTTCCAGTCCAAACCCAGTTGATTCAAATGACTTAGACAAGAGGCAAAAACACAAGTCCTCAAGAATCACCACTTCTCATCAAGCAACTGTAGCACCAAATCCCAACTCCAATCTTTTCGACGATCATCTCGAGAAAGAGACTGATTCTACTTCATATTCTTCATCTTCAAGTTCATATTCATGGTCATCAAGAAACAGATACAAGAATGATTTCAGGGACTCAGGTGGGATTGAGAATCAAACAGTGGAAGAGTTGGAGAATTATGCTGTATACAAGGCTGAGGAGACAACTAACTCAGTCAACAACTGCCTGAAGATTGCTGAGGACATGAGAGAAGATGCAACCAAAACCTTAGTTGCCTTGCATCAACAGGGTGAGCAAATCACTCGGACCCATGTGGTTACTGCTGATATCGACAACGATCTTAGTCGGGTACGTGGAACGAATTAACCGCCATATCAATGACATGTTTGAAGTGATTTTGAAATGGTTAAAATCACTTTTGTCTTGTTCAATAATCATTCAAAATTAGTTTCATGTTTGGTTTACACTTTTAAATGTGGTTGTCATCCTATTAAAATTGATTTTAAACGATTAAAAACTTGTAAAAAAAAAACTCTTGAACCTGCCCTTTTCTTTCCTTATGTCCTGAATAAACAAACCTATGCACTAAATATAGGCTTAGCTTCATTTATGGATGCTTTTTGTAAAGATATTGAAATTTATTTTTGGTTATGAAATGATCATCAATATTTTACGATGTTCATTGCAGGGTGAGAAACTTCTTGGAAGCTTAGGAGGAATATTCTCTAAGACTTGGAAGCCAAAGAAAGGTCGTCAGATAACAGGCCCTGTTATCTTTGAAGGTAATATTTTCAAATTTTGGCTGTGGAAGAAAATAATTAAGAAAAATAGAACTGCTTTTTATAACTTCTTTTTTCTTTGTTTTATTATTTAATGATCTAAAAGTTTTACATGAATATATATATTTTTTTAATTAAATATTGATTTTTTATGTTATACATTTTTTAAAAAATAAATAAATAGAACAATACGTTTATTTATGTTTTGGAAAAAAAACTTCAAACTGAAAAAAAAAAATAGAACAGTTAATAAACACTTATGTTTCTATTCCTTACTCTAACTCGGGATGTACTAGAAAAAAGACTCAATTATGTAACGATAAAACTAAAAAAATACTATTTACCAAAAACATATAATCTATTGTTGAACGGACCTTACCGCAAATGAATATATTTTGTTTTTTCATTCTCAATAATAAATGAAACTTCTAAAAAAAATTATAGTGAAAATGTTTGGACAAATAAGACTCGTGCTATCTTTTTTAATACTAATTACCCATAAATTGAAGATAAAATTTTTTTAATACTAATTACCCATAAATTGAAGATAAAATTGAGTCAGAAGATCTAAAAACAAGAAACATAAACATTATCTCCGTAATTTTAAATTATTTGAACTAATTTGAACAAACTTAATCTATAGAACCAAAAAGGTTTCAACTTTCCCACTAATCAGATGGATTAAGTTGATGGCCAATTAAGTTTTTAAAAATATTATTAAATTTTTAATAAATAAATTTGATTTGCCTTGGTTGCAGGGGATCAGGCTGTTATAAGCAAGGGCAACCATTTGGAGCAAAGGGAGAAGTTGGGGTTAAGTCGGGCACCAAATCCACGGTCCTCACGTGCCCCACATCCTGAGCCAACCAACTCACTTCAGAAAGTTGAGGTTTGTAGTTATTCAGTTATAGTGCTTCTCATGGAAGTACTTACAGAAAAAGCACTTCACTTACAATACACAAACGTGAAGGGAGCAGAATAGAAGAATATTTGAAAACACTTAACAAGAAATTTGTTCTAAACCAGGTGGAAAAGTTCAAGCAAGAGGATGCTCTTTCAGATTTGAGCAATATATTGGGAGAGCTTAAAGAAATGGCTGTTGATATGGGATCAGAACTAGACAGGTACCTTTTTTTTTTCTTCTTAGAATCACTTCTCAAATTGCCCAGGAGAAATAGAAAAAATATGAATGAGAAATAAAAAAAAACTGAGAACGTTACCAAATGGGTAAAAATGCCAAGTTTTCTTTCTGTTTCTTTTATTTTTGTTAAACTCAGCTGGAATCAATCTCATTATTTCAGGCAAAATAAGGCTTTGGATAACTTGTTTGATGATGCCAATGAGGTCACGATTCGTGTCGATAATGCTAACCAGCGTGCTCGTCATCTGCTTAGAAAGTAGATTGCAAATTTGCAAGGTCTCATCTCATTTTTGCCATCAAGAAATATGATGGTATATGCTTGACTGGATTGGCTTCCCTTCTGGTTTATAGTCTCTTCTTCCAGTATGTCTTTCTTCATCCAAAAAGAAACAAACAGTTTTTTTTTTTTTTCAAAACAGTTGTGAAATAGTTTTGTTATTGCAAGGGCA

mRNA sequence

ATGTCACCTTTTAGGAAATCCCCTATAAATGTTGCTAGGCACGGCACAAGCCAACCTGCTAATCCTGCTCGTTCCAGTCCAAACCCAGTTGATTCAAATGACTTAGACAAGAGGCAAAAACACAAGTCCTCAAGAATCACCACTTCTCATCAAGCAACTGTAGCACCAAATCCCAACTCCAATCTTTTCGACGATCATCTCGAGAAAGAGACTGATTCTACTTCATATTCTTCATCTTCAAGTTCATATTCATGGTCATCAAGAAACAGATACAAGAATGATTTCAGGGACTCAGGTGGGATTGAGAATCAAACAGTGGAAGAGTTGGAGAATTATGCTGTATACAAGGCTGAGGAGACAACTAACTCAGTCAACAACTGCCTGAAGATTGCTGAGGACATGAGAGAAGATGCAACCAAAACCTTAGTTGCCTTGCATCAACAGGGTGAGCAAATCACTCGGACCCATGTGGGTGAGAAACTTCTTGGAAGCTTAGGAGGAATATTCTCTAAGACTTGGAAGCCAAAGAAAGGTCGTCAGATAACAGGCCCTGTTATCTTTGAAGGGGATCAGGCTGTTATAAGCAAGGGCAACCATTTGGAGCAAAGGGAGAAGTTGGGGTTAAGTCGGGCACCAAATCCACGGTCCTCACGTGCCCCACATCCTGAGCCAACCAACTCACTTCAGAAAGTTGAGGTGGAAAAGTTCAAGCAAGAGGATGCTCTTTCAGATTTGAGCAATATATTGGGAGAGCTTAAAGAAATGGCTGTTGATATGGGATCAGAACTAGACAGGCAAAATAAGGCTTTGGATAACTTGTTTGATGATGCCAATGAGGTCACGATTCGTGTCGATAATGCTAACCAGCGTGCTCGTCATCTGCTTAGAAAGTAGATTGCAAATTTGCAAGGTCTCATCTCATTTTTGCCATCAAGAAATATGATGGTATATGCTTGACTGGATTGGCTTCCCTTCTGGTTTATAGTCTCTTCTTCCAGTATGTCTTTCTTCATCCAAAAAGAAACAAACAGTTTTTTTTTTTTTTCAAAACAGTTGTGAAATAGTTTTGTTATTGCAAGGGCA

Coding sequence (CDS)

ATGTCACCTTTTAGGAAATCCCCTATAAATGTTGCTAGGCACGGCACAAGCCAACCTGCTAATCCTGCTCGTTCCAGTCCAAACCCAGTTGATTCAAATGACTTAGACAAGAGGCAAAAACACAAGTCCTCAAGAATCACCACTTCTCATCAAGCAACTGTAGCACCAAATCCCAACTCCAATCTTTTCGACGATCATCTCGAGAAAGAGACTGATTCTACTTCATATTCTTCATCTTCAAGTTCATATTCATGGTCATCAAGAAACAGATACAAGAATGATTTCAGGGACTCAGGTGGGATTGAGAATCAAACAGTGGAAGAGTTGGAGAATTATGCTGTATACAAGGCTGAGGAGACAACTAACTCAGTCAACAACTGCCTGAAGATTGCTGAGGACATGAGAGAAGATGCAACCAAAACCTTAGTTGCCTTGCATCAACAGGGTGAGCAAATCACTCGGACCCATGTGGGTGAGAAACTTCTTGGAAGCTTAGGAGGAATATTCTCTAAGACTTGGAAGCCAAAGAAAGGTCGTCAGATAACAGGCCCTGTTATCTTTGAAGGGGATCAGGCTGTTATAAGCAAGGGCAACCATTTGGAGCAAAGGGAGAAGTTGGGGTTAAGTCGGGCACCAAATCCACGGTCCTCACGTGCCCCACATCCTGAGCCAACCAACTCACTTCAGAAAGTTGAGGTGGAAAAGTTCAAGCAAGAGGATGCTCTTTCAGATTTGAGCAATATATTGGGAGAGCTTAAAGAAATGGCTGTTGATATGGGATCAGAACTAGACAGGCAAAATAAGGCTTTGGATAACTTGTTTGATGATGCCAATGAGGTCACGATTCGTGTCGATAATGCTAACCAGCGTGCTCGTCATCTGCTTAGAAAGTAG

Protein sequence

MSPFRKSPINVARHGTSQPANPARSSPNPVDSNDLDKRQKHKSSRITTSHQATVAPNPNSNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEETTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVGEKLLGSLGGIFSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNANQRARHLLRK

Homology

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BLAST of Lsi06G014310 vs. ExPASy Swiss-Prot
Match: Q9S7P9 (SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.7e-72
Identity = 168/310 (54.19%), Postives = 209/310 (67.42%), Query Frame = 0


Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           M   RKSP N+ +H +    +   S PNP DS+D  D +     S+ TTS  +       
Sbjct: 1   MFGLRKSPANLPKHNS---VDLKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLA---DM 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           +N F     ++ DS   SSS  S   +S+ +YKN+FRDSGGIENQ+V+ELE YAVYKAEE
Sbjct: 61  TNPFGGERVQKGDS---SSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTH-----------VGEKLLGSLGGI 180
           TT SV  CLK+AED+R DAT+TLV LH QGEQITRTH            GEKLLGSLGG+
Sbjct: 121 TTKSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGM 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRS-SRAPHPEPTNS 240
           FSKTWKPKK R I GPV+   D +   + NHLE+REKLGL+ AP  +S +R P PE  ++
Sbjct: 181 FSKTWKPKKTRPINGPVVTR-DDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADA 240

Query: 241 LQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNA 298
            Q+VE+EK KQ+D LSDLS+ILGELK MAVDMGSE+++QNK LD+L DD +E+  RV  +
Sbjct: 241 YQRVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQS 300

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BLAST of Lsi06G014310 vs. ExPASy Swiss-Prot
Match: Q9LMG8 (Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNAP30 PE=3 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 9.9e-65
Identity = 137/220 (62.27%), Postives = 161/220 (73.18%), Query Frame = 0


Query: 89  NRYKNDFRDSGGIENQTVEELENYAVYKAEETTNSVNNCLKIAEDMREDATKTLVALHQQ 148
           ++YKN F DSGG+++QT EELE YAVYKAEETT  VNNCLKIAED+R D  +TL  LHQQ
Sbjct: 46  DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKGVNNCLKIAEDIRSDGARTLEMLHQQ 105

Query: 149 GEQITRTHV-----------GEKLLGSLGGIFSKTWKPKKGRQITGPVIFEGDQAVISKG 208
           GEQI RTH            GEKLL +LGG+FSK WKPKK + ITGP+I   D+      
Sbjct: 106 GEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKPWKPKKTKNITGPMI-TPDKPSKKSE 165

Query: 209 NHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKFKQEDALSDLSNILGELKEMAV 268
           NH E+REKLGL  A    SS+    +PTN+LQKVE EK KQ+D LSDLS+ILG+LK MAV
Sbjct: 166 NHKEEREKLGLG-AKGRSSSQPALDQPTNALQKVEQEKAKQDDGLSDLSDILGDLKSMAV 225

Query: 269 DMGSELDRQNKALDNLFDDANEVTIRVDNANQRARHLLRK 298
           DMGSE+D+QNKALD+L DD +E+  RV  ANQRARHLL K
Sbjct: 226 DMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARHLLSK 263

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BLAST of Lsi06G014310 vs. ExPASy Swiss-Prot
Match: Q9SD96 (SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 3.3e-52
Identity = 124/252 (49.21%), Postives = 165/252 (65.48%), Query Frame = 0


Query: 57  NPNSNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYK 116
           N + N FDD  E     +               R+ +  + SGG ENQTV+ELE+YAVY 
Sbjct: 5   NSSWNPFDDEKEAAKSFSLNPFDDDDDDKEVEKRFTSSLKPSGGKENQTVQELESYAVYN 64

Query: 117 AEETTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHV-----------GEKLLGSL 176
           +EETT +V  CLK+AE++R DA+KTLV L++QG+QITRTH            GEK+LG L
Sbjct: 65  SEETTKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRL 124

Query: 177 GGIFSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPT 236
           GG+FS+TWKPKK R ITGPVI +GD     K   L+ REKLGL+ +  P+S     PE  
Sbjct: 125 GGVFSRTWKPKKSRSITGPVITKGDSPK-RKVIDLKTREKLGLNPSLKPKSKTL--PEAV 184

Query: 237 NSLQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVD 296
           ++ QK ++   KQ++AL+DLS +LGELK MAVDMG+ ++RQ   LD+L D+A+E+  RV 
Sbjct: 185 DAYQKTQIA--KQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVK 244

Query: 297 NANQRARHLLRK 298
            +NQRAR+LLRK
Sbjct: 245 QSNQRARYLLRK 251

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BLAST of Lsi06G014310 vs. ExPASy Swiss-Prot
Match: Q17QQ3 (Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.7e-11
Identity = 60/213 (28.17%), Postives = 99/213 (46.48%), Query Frame = 0


Query: 106 VEELENYAVYKAEETTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVG------- 165
           +EE++  A   A+E+  S    L++ E+ ++   +TLV L +QGEQ+ R   G       
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 166 ----EKLLGSLGGI------------FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQ 225
               EK L  LG               S  +K   G    G V  +  + V       ++
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVV-------DE 130

Query: 226 REKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSE 285
           RE++ +S     R         TN  ++ E+     ++ L  +S I+G L+ MA+DMG+E
Sbjct: 131 REQMAISGGFIRR--------VTNDARENEM-----DENLEQVSGIIGNLRHMALDMGNE 190

Query: 286 LDRQNKALDNLFDDANEVTIRVDNANQRARHLL 296
           +D QN+ +D + + A+    R+D ANQRA  +L
Sbjct: 191 IDTQNRQIDRIMEKADSNKTRIDEANQRATKML 203

[Close]

BLAST of Lsi06G014310 vs. ExPASy Swiss-Prot
Match: P60878 (Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.7e-11
Identity = 60/213 (28.17%), Postives = 99/213 (46.48%), Query Frame = 0


Query: 106 VEELENYAVYKAEETTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVG------- 165
           +EE++  A   A+E+  S    L++ E+ ++   +TLV L +QGEQ+ R   G       
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 166 ----EKLLGSLGGI------------FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQ 225
               EK L  LG               S  +K   G    G V  +  + V       ++
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVV-------DE 130

Query: 226 REKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSE 285
           RE++ +S     R         TN  ++ E+     ++ L  +S I+G L+ MA+DMG+E
Sbjct: 131 REQMAISGGFIRR--------VTNDARENEM-----DENLEQVSGIIGNLRHMALDMGNE 190

Query: 286 LDRQNKALDNLFDDANEVTIRVDNANQRARHLL 296
           +D QN+ +D + + A+    R+D ANQRA  +L
Sbjct: 191 IDTQNRQIDRIMEKADSNKTRIDEANQRATKML 203

[Close]

BLAST of Lsi06G014310 vs. ExPASy TrEMBL
Match: A0A0A0LRS5 (t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G012100 PE=4 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 1.9e-143
Identity = 277/309 (89.64%), Postives = 288/309 (93.20%), Query Frame = 0


Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MSPFRKSPINV RHG+SQPAN ARSSPNPV+SND LDK+QK +SSRIT+SHQAT APNP 
Sbjct: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQK-QSSRITSSHQATAAPNPK 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLEK TDS+SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELE+YAVYKAEE
Sbjct: 61  SALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHV-----------GEKLLGSLGGI 180
           TTNSVNNCLKIAED+REDATKTLVALHQQGEQITRTHV           GEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 298
           QKVEVEK KQ+DALSDLSNILGELKEMAVDMGSELD+QNKALD+LFDDANEVTIRVDNAN
Sbjct: 241 QKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNAN 300

[Close]

BLAST of Lsi06G014310 vs. ExPASy TrEMBL
Match: A0A5A7US66 (Putative SNAP25-like proteinous protein SNAP30 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002820 PE=4 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 3.0e-141
Identity = 276/309 (89.32%), Postives = 285/309 (92.23%), Query Frame = 0


Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MSPFRKSPINV RH ++QPANPARSSPN V+SND LDKRQK +SSRIT+SHQ T APNP 
Sbjct: 1   MSPFRKSPINVVRHSSNQPANPARSSPNLVNSNDNLDKRQK-QSSRITSSHQETAAPNPK 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLEK TDS SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE
Sbjct: 61  SALFDDHLEKGTDS-SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHV-----------GEKLLGSLGGI 180
           TTNSVNNCLKIAED+REDATKTLVALHQQGEQITRTH+           GEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 298
           QKVEVEK KQEDALSDLSNILGELKEMAVDMGSELD+QN+ALDNLFDDANEVTIRVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKEMAVDMGSELDKQNRALDNLFDDANEVTIRVDNAN 300

[Close]

BLAST of Lsi06G014310 vs. ExPASy TrEMBL
Match: A0A1S3BVT2 (putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC103494024 PE=4 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 3.0e-141
Identity = 276/309 (89.32%), Postives = 285/309 (92.23%), Query Frame = 0


Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MSPFRKSPINV RH ++QPANPARSSPN V+SND LDKRQK +SSRIT+SHQ T APNP 
Sbjct: 1   MSPFRKSPINVVRHSSNQPANPARSSPNLVNSNDNLDKRQK-QSSRITSSHQETAAPNPK 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLEK TDS SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE
Sbjct: 61  SALFDDHLEKGTDS-SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHV-----------GEKLLGSLGGI 180
           TTNSVNNCLKIAED+REDATKTLVALHQQGEQITRTH+           GEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 298
           QKVEVEK KQEDALSDLSNILGELKEMAVDMGSELD+QN+ALDNLFDDANEVTIRVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKEMAVDMGSELDKQNRALDNLFDDANEVTIRVDNAN 300

[Close]

BLAST of Lsi06G014310 vs. ExPASy TrEMBL
Match: A0A6J1E197 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC111429687 PE=4 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.7e-139
Identity = 266/309 (86.08%), Postives = 282/309 (91.26%), Query Frame = 0


Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MS FRKSPI  ARHG+SQPANPARS+  P+DSND LDKRQKHKSSRIT+SHQATVAPNP+
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSTSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLEKETD    SSS SSYSWSSRNRYKNDFRDSGG+++QTVEELENYA YKAEE
Sbjct: 61  SNLFDDHLEKETD----SSSHSSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHV-----------GEKLLGSLGGI 180
           TTN+VNNCLKIAEDMREDAT TLVALH QGEQITRTH+           GEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRE+LGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 298
           QKVEVEK KQEDALSDLSNILGELK MAVDMG+ELDRQNKALDNLFDDA+E+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNAN 300

[Close]

BLAST of Lsi06G014310 vs. ExPASy TrEMBL
Match: A0A6J1JJU0 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC111485094 PE=4 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 4.5e-137
Identity = 264/309 (85.44%), Postives = 280/309 (90.61%), Query Frame = 0


Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MS FRKSPI  ARHG+SQPA PARS+  P+DSND  DKRQKHKSSRIT+SHQATVAPNP 
Sbjct: 1   MSHFRKSPIRFARHGSSQPAYPARSTSQPIDSNDNSDKRQKHKSSRITSSHQATVAPNPI 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLEKETD    SSS SSYSWSSRNRYKNDFRDSGG+++QTVEELENYA YKAEE
Sbjct: 61  SNLFDDHLEKETD----SSSHSSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHV-----------GEKLLGSLGGI 180
           TTN+VNNCLKIAEDMREDATKTLVALH QGEQITRTH+           GEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATKTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRE+LGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 298
           QKVEVEK KQEDALSDLSNILGELK MAVDMG+ELDRQNKALD+LFDDA+E+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDHLFDDADEITVRVDNAN 300

[Close]

BLAST of Lsi06G014310 vs. NCBI nr
Match: XP_038879751.1 (SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879752.1 SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879753.1 SNAP25 homologous protein SNAP33-like [Benincasa hispida])

HSP 1 Score: 531.9 bits (1369), Expect = 3.4e-147
Identity = 281/309 (90.94%), Postives = 292/309 (94.50%), Query Frame = 0


Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MSPFRKSPINVARHG+SQP N AR SPNPVDSND LDK+QK+KSSRIT+SHQATVAPNP+
Sbjct: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLE+ET+S SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE
Sbjct: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHV-----------GEKLLGSLGGI 180
           TTN+VNNCLKIAEDMREDATKTLVALHQQGEQITRTHV           GEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRS RAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 298
           +KVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALD+LFDDA+EVTIRVDNAN
Sbjct: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300

[Close]

BLAST of Lsi06G014310 vs. NCBI nr
Match: XP_004138184.1 (SNAP25 homologous protein SNAP33 [Cucumis sativus] >KGN63709.1 hypothetical protein Csa_013922 [Cucumis sativus])

HSP 1 Score: 518.5 bits (1334), Expect = 3.9e-143
Identity = 277/309 (89.64%), Postives = 288/309 (93.20%), Query Frame = 0


Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MSPFRKSPINV RHG+SQPAN ARSSPNPV+SND LDK+QK +SSRIT+SHQAT APNP 
Sbjct: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQK-QSSRITSSHQATAAPNPK 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLEK TDS+SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELE+YAVYKAEE
Sbjct: 61  SALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHV-----------GEKLLGSLGGI 180
           TTNSVNNCLKIAED+REDATKTLVALHQQGEQITRTHV           GEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 298
           QKVEVEK KQ+DALSDLSNILGELKEMAVDMGSELD+QNKALD+LFDDANEVTIRVDNAN
Sbjct: 241 QKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNAN 300

[Close]

BLAST of Lsi06G014310 vs. NCBI nr
Match: XP_008453247.1 (PREDICTED: putative SNAP25 homologous protein SNAP30 [Cucumis melo] >KAA0057978.1 putative SNAP25-like proteinous protein SNAP30 [Cucumis melo var. makuwa])

HSP 1 Score: 511.1 bits (1315), Expect = 6.2e-141
Identity = 276/309 (89.32%), Postives = 285/309 (92.23%), Query Frame = 0


Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MSPFRKSPINV RH ++QPANPARSSPN V+SND LDKRQK +SSRIT+SHQ T APNP 
Sbjct: 1   MSPFRKSPINVVRHSSNQPANPARSSPNLVNSNDNLDKRQK-QSSRITSSHQETAAPNPK 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLEK TDS SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE
Sbjct: 61  SALFDDHLEKGTDS-SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHV-----------GEKLLGSLGGI 180
           TTNSVNNCLKIAED+REDATKTLVALHQQGEQITRTH+           GEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 298
           QKVEVEK KQEDALSDLSNILGELKEMAVDMGSELD+QN+ALDNLFDDANEVTIRVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKEMAVDMGSELDKQNRALDNLFDDANEVTIRVDNAN 300

[Close]

BLAST of Lsi06G014310 vs. NCBI nr
Match: XP_022921394.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921396.1 putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921397.1 putative SNAP25 homologous protein SNAP30 [Cucurbita moschata])

HSP 1 Score: 504.2 bits (1297), Expect = 7.6e-139
Identity = 266/309 (86.08%), Postives = 282/309 (91.26%), Query Frame = 0


Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MS FRKSPI  ARHG+SQPANPARS+  P+DSND LDKRQKHKSSRIT+SHQATVAPNP+
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSTSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLEKETD    SSS SSYSWSSRNRYKNDFRDSGG+++QTVEELENYA YKAEE
Sbjct: 61  SNLFDDHLEKETD----SSSHSSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHV-----------GEKLLGSLGGI 180
           TTN+VNNCLKIAEDMREDAT TLVALH QGEQITRTH+           GEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRE+LGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 298
           QKVEVEK KQEDALSDLSNILGELK MAVDMG+ELDRQNKALDNLFDDA+E+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNAN 300

[Close]

BLAST of Lsi06G014310 vs. NCBI nr
Match: XP_023516514.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_023516515.1 putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_023516516.1 putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 503.1 bits (1294), Expect = 1.7e-138
Identity = 265/309 (85.76%), Postives = 281/309 (90.94%), Query Frame = 0


Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MS FRKSPI  ARHG+SQPANPARS   P+DSND LDKRQKHKSSRIT+SHQATVAPNP+
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSPSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLEKETD    SSS SSYSWSSRNRYKNDFRDSGG+++QTVEELENYA YKAEE
Sbjct: 61  SNLFDDHLEKETD----SSSHSSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHV-----------GEKLLGSLGGI 180
           TTN+VNNCLKIAEDMREDAT TLVALH QGEQITRTH+           GEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGP+IFEGDQAVISKGNHLEQRE+LGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPIIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 298
           QKVEVEK KQEDALSDLSNILGELK MAVDMG+ELDRQNKALDNLFDDA+E+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNAN 300

[Close]

BLAST of Lsi06G014310 vs. TAIR 10
Match: AT5G61210.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 33 )

HSP 1 Score: 274.2 bits (700), Expect = 1.2e-73
Identity = 168/310 (54.19%), Postives = 209/310 (67.42%), Query Frame = 0


Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           M   RKSP N+ +H +    +   S PNP DS+D  D +     S+ TTS  +       
Sbjct: 1   MFGLRKSPANLPKHNS---VDLKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLA---DM 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           +N F     ++ DS   SSS  S   +S+ +YKN+FRDSGGIENQ+V+ELE YAVYKAEE
Sbjct: 61  TNPFGGERVQKGDS---SSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTH-----------VGEKLLGSLGGI 180
           TT SV  CLK+AED+R DAT+TLV LH QGEQITRTH            GEKLLGSLGG+
Sbjct: 121 TTKSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGM 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRS-SRAPHPEPTNS 240
           FSKTWKPKK R I GPV+   D +   + NHLE+REKLGL+ AP  +S +R P PE  ++
Sbjct: 181 FSKTWKPKKTRPINGPVVTR-DDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADA 240

Query: 241 LQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNA 298
            Q+VE+EK KQ+D LSDLS+ILGELK MAVDMGSE+++QNK LD+L DD +E+  RV  +
Sbjct: 241 YQRVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQS 300

[Close]

BLAST of Lsi06G014310 vs. TAIR 10
Match: AT1G13890.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 30 )

HSP 1 Score: 248.4 bits (633), Expect = 7.0e-66
Identity = 137/220 (62.27%), Postives = 161/220 (73.18%), Query Frame = 0


Query: 89  NRYKNDFRDSGGIENQTVEELENYAVYKAEETTNSVNNCLKIAEDMREDATKTLVALHQQ 148
           ++YKN F DSGG+++QT EELE YAVYKAEETT  VNNCLKIAED+R D  +TL  LHQQ
Sbjct: 46  DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKGVNNCLKIAEDIRSDGARTLEMLHQQ 105

Query: 149 GEQITRTHV-----------GEKLLGSLGGIFSKTWKPKKGRQITGPVIFEGDQAVISKG 208
           GEQI RTH            GEKLL +LGG+FSK WKPKK + ITGP+I   D+      
Sbjct: 106 GEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKPWKPKKTKNITGPMI-TPDKPSKKSE 165

Query: 209 NHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKFKQEDALSDLSNILGELKEMAV 268
           NH E+REKLGL  A    SS+    +PTN+LQKVE EK KQ+D LSDLS+ILG+LK MAV
Sbjct: 166 NHKEEREKLGLG-AKGRSSSQPALDQPTNALQKVEQEKAKQDDGLSDLSDILGDLKSMAV 225

Query: 269 DMGSELDRQNKALDNLFDDANEVTIRVDNANQRARHLLRK 298
           DMGSE+D+QNKALD+L DD +E+  RV  ANQRARHLL K
Sbjct: 226 DMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARHLLSK 263

[Close]

BLAST of Lsi06G014310 vs. TAIR 10
Match: AT5G07880.1 (synaptosomal-associated protein SNAP25-like 29 )

HSP 1 Score: 206.8 bits (525), Expect = 2.3e-53
Identity = 124/252 (49.21%), Postives = 165/252 (65.48%), Query Frame = 0


Query: 57  NPNSNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYK 116
           N + N FDD  E     +               R+ +  + SGG ENQTV+ELE+YAVY 
Sbjct: 5   NSSWNPFDDEKEAAKSFSLNPFDDDDDDKEVEKRFTSSLKPSGGKENQTVQELESYAVYN 64

Query: 117 AEETTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHV-----------GEKLLGSL 176
           +EETT +V  CLK+AE++R DA+KTLV L++QG+QITRTH            GEK+LG L
Sbjct: 65  SEETTKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRL 124

Query: 177 GGIFSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPT 236
           GG+FS+TWKPKK R ITGPVI +GD     K   L+ REKLGL+ +  P+S     PE  
Sbjct: 125 GGVFSRTWKPKKSRSITGPVITKGDSPK-RKVIDLKTREKLGLNPSLKPKSKTL--PEAV 184

Query: 237 NSLQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVD 296
           ++ QK ++   KQ++AL+DLS +LGELK MAVDMG+ ++RQ   LD+L D+A+E+  RV 
Sbjct: 185 DAYQKTQIA--KQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVK 244

Query: 297 NANQRARHLLRK 298
            +NQRAR+LLRK
Sbjct: 245 QSNQRARYLLRK 251

[Close]

BLAST of Lsi06G014310 vs. TAIR 10
Match: AT3G09740.1 (syntaxin of plants 71 )

HSP 1 Score: 42.7 bits (99), Expect = 5.8e-04
Identity = 26/70 (37.14%), Postives = 35/70 (50.00%), Query Frame = 0


Query: 223 EPTNSLQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTI 282
           E +   Q+ E+ K KQE  L  +S  L  LK MA DM  ELDRQ   +D +    +  T 
Sbjct: 163 ESSQFRQEYEMRKIKQEQGLDMISEGLDALKNMASDMNEELDRQVPLMDEIDTKVDRATS 222

Query: 283 RVDNANQRAR 293
            + N N R +
Sbjct: 223 DLKNTNVRLK 232

The following BLAST results are available for this feature:

BLAST of Lsi06G014310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7P9	1.7e-72	54.19	SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 ...	[more]
Q9LMG8	9.9e-65	62.27	Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNA...	[more]
Q9SD96	3.3e-52	49.21	SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 ...	[more]
Q17QQ3	1.7e-11	28.17	Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1	[more]
P60878	1.7e-11	28.17	Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1	[more]

BLAST of Lsi06G014310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRS5	1.9e-143	89.64	t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=365...	[more]
A0A5A7US66	3.0e-141	89.32	Putative SNAP25-like proteinous protein SNAP30 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3BVT2	3.0e-141	89.32	putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC10349402...	[more]
A0A6J1E197	3.7e-139	86.08	putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1JJU0	4.5e-137	85.44	putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of Lsi06G014310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879751.1	3.4e-147	90.94	SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879752.1 SNAP25...	[more]
XP_004138184.1	3.9e-143	89.64	SNAP25 homologous protein SNAP33 [Cucumis sativus] >KGN63709.1 hypothetical prot...	[more]
XP_008453247.1	6.2e-141	89.32	PREDICTED: putative SNAP25 homologous protein SNAP30 [Cucumis melo] >KAA0057978....	[more]
XP_022921394.1	7.6e-139	86.08	putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921396.1 p...	[more]
XP_023516514.1	1.7e-138	85.76	putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_02351...	[more]

BLAST of Lsi06G014310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G61210.1	1.2e-73	54.19	soluble N-ethylmaleimide-sensitive factor adaptor protein 33	[more]
AT1G13890.1	7.0e-66	62.27	soluble N-ethylmaleimide-sensitive factor adaptor protein 30	[more]
AT5G07880.1	2.3e-53	49.21	synaptosomal-associated protein SNAP25-like 29	[more]
AT3G09740.1	5.8e-04	37.14	syntaxin of plants 71	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 270..297
None	No IPR available	COILS	Coil	Coil	coord: 128..148
None	No IPR available	GENE3D	1.20.5.110		coord: 100..161 e-value: 1.1E-14 score: 56.1
None	No IPR available	GENE3D	1.20.5.110		coord: 217..297 e-value: 1.4E-20 score: 75.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 46..60
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..97
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 11..30
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 198..227
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 73..89
None	No IPR available	PANTHER	PTHR19305	SYNAPTOSOMAL ASSOCIATED PROTEIN	coord: 22..297
None	No IPR available	PANTHER	PTHR19305:SF30	TARGET SNARE COILED-COIL DOMAIN-CONTAINING PROTEIN-RELATED	coord: 22..297
None	No IPR available	CDD	cd15841	SNARE_Qc	coord: 237..293 e-value: 2.40338E-12 score: 59.1092
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 235..295
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 104..157
IPR000727	Target SNARE coiled-coil homology domain	SMART	SM00397	tSNARE_6	coord: 227..294 e-value: 3.0E-8 score: 43.4
IPR000727	Target SNARE coiled-coil homology domain	PROSITE	PS50192	T_SNARE	coord: 237..294 score: 14.072053
IPR044766	NPSN/SNAP25-like, N-terminal SNARE domain	CDD	cd15861	SNARE_SNAP25N_23N_29N_SEC9N	coord: 111..156 e-value: 2.48187E-14 score: 64.5167

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G014310.1	Lsi06G014310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061025	membrane fusion
biological_process	GO:0015031	protein transport
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0031201	SNARE complex
molecular_function	GO:0005484	SNAP receptor activity