Lsi06G014210 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G014210
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionFlavonoid 3' hydroxylase
Locationchr06: 24754847 .. 24760856 (-)
RNA-Seq ExpressionLsi06G014210
SyntenyLsi06G014210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAGACCAAGGATTCATCAAATAAATTTCGATCGTAGATCCTAACTACTTCAAAGTGGAAATTACAAATTTACCCATAAATCATCTCAACTCCATTTCCCTTTTAAGCACCGCCTTTCCTTCCCTTCCCTTCACTGAAATTCTCTATAACATATATATGGACGAAATTTCTCTCATCGTCGCCGCCGTTGTAGTAACCTTGGCCTACGCCATCCTCCTCCACCGCGCTTTCTTCCTCCGCCGCAGATTCTGCCTCCCGCCTGGTCCAAAACCATGGCCGATCGTAGGAAACATGCCGCAAATCGGCGGAGCGCCTCTCCACTCCCTCCTCGCCGCCATGGCCGGCGAGTACGGACCTCTGATGTACCTCCGATTAGGATCCGTTGACGTGGTCGTGGCGGCGTCGGCGTCGGTGGCGGAGCAACTCTTGAAGATTCACGGCACTAATTTCCTTAGCCGGCCGCCGAACGCCGGCGCAAAATATATTGCGTATAATAATCGGGATATGGTTTTTGCACCGTACGGTCCACGGTGGCGATTACTCCGGAAGATCAGCACCGTCCATCTGTTCTCCTCAAAAGCCCTAGATGATCATCGCCGCATACGGGAGGTTCGTCCTGATATTTTTATTTTTTATTTTTCTTTCTATTTATATCTATGATATGAATGCCTGATTTTCACCCTAATTTTTCTTCTCAGAAAATAGGTTTTCGTATTTAGAAAATAATAATAATAATAATAAGGCAAGTTTTAAAAATAGAAAAGTACAAAATATCAAAATTTTTAAATAGTTTTGATAGGAATGATAGGAATCTATCGGTGATAGGAATCTATCAATGTCTATCGTATTTTTTTTTATTTGTGAAAATTTTTCCTAATAATAATAAGATATTTGTTTATTTATTTATTTTGATTTAAAGACCAATTTCATTAAAAATTAGAAATCAACTGCATCTCCCTTAAAATAATGGAAGAAAGTCAACTAGGAGATAAAGACTTTCTATTTTTTAACACACAAACACAATATAAAAACAATTCATTTTTTAGAAAAAAGAAAAATAAATACAGTTTTTTAGATAGGGTGGTTAAATCAGCAATTGACCATATTATATCTTAACACTCTCCCTCAAAACTTAAGCTGATAGGTGAAGACAAATTTAATAGCCAAGCAAGTGGAATTAGATATTAATTGGGGAGGAAATAACAATGCATGGCTTGAACACAGGACATCCTGGACCACCAGCTCTGATACCATGTTAAATCACTGATTAATCTAAAAGCTTAAGCTGATAGGTAAAGGACAAGTTTAATATCATATCTTAACAAAGACGATGCTAAGGATAAATTTAAGACTTTTGATGTGAGTTTTGAACAACCACGACGATGCTAAGGACAAATTTAAGACTTTTGATGTGAGTTTTGAACGACCATGACGGTGCTAAAGACAAATTTAAGACTTTTGATGTGAGTTTTGAACAACTATTCCCAAATCTCCTTCGATACGACATCTTAAACTATTAAATATAATTATATTATTTAATAAATATTTTAATGTTATCTTTAACTATTTAATATAAAAATTTATTATTAATTATTAATATTATAATTAATTAAATTAAATCATTAAATAAATATTTAATTAGTGTTTATATAAACATAAGTAGTGATTCTACTTATGTCACTTCATATTTTTCATCGACTTTGCGGATGTGATTAATTAGAAATGTTATAGATTATTTTTAAAGGTATATTTCGTCAAAGAAATAAGAGTTGATGGAGTAACTGTAAAAAATACCTAACTTTTAAAAGTTTAAAAAATAACTTTCAAAATGAATTTAAAAATACTGTTAGCATTAGTTTTGGATAGAAATCGTGTCCGTAATATTTGAACTTTCAAAATTAAAAAAATATCTCTAAATTAAGAAAAATAAAAATAAATAATACCCTTACTTTATATCTAAAAATAAATAAAATTGCACGCTAGAAAATAATAGATGCATCAAGGAATTTTAGGACGTAAATATTTTATCAAAATTGTATATTAATAGTTAGATATGTTTTAATAATCAAACACATTTTTTATTTGACTATTTATTAGAAAAGTGAGAATATATATATATATATATATATAAATATCAATATAGTTTATTTTTTTTAACCTAATAAAACTTCAAACAAATTGTTTTTTAACTCTTTTTGTAATTTTATGATATATTTTACTCAAAATGTAAATTTAGAGACCTTTTTTAAAATTTAGCCTAACATCAATTTTAGTGTACTGTGTTACACAACGAATGATTAGATAACATGAGTATGAAAAAATAATGGAACATTTAATTTTTGTCGATAATTTATGTAAAGATGAAACTATACTTTCTTTAAAATCGATCGAGAGTACGTGTAAGCCTATAAACCAAATGTATTTTATAATTATTATTTTCTTTTTCCTTTGTGTAAATTTTGTTTCTTGATAGAATTTAAGGTGGAAAAATAACAATAATAAAAAAAAATATAAAATCATATAGTTTTCTGATATAATTTTTAAACCTAAGACAAGTTAAGTAGGGGGTCATCGTTATAATATAACCATAGTAAAAAGAAAATTATAATTTATGCTAAATTTGAAAACAATGAGAAAATGTGTGACTTTCGTTTGATAATAGATATTAAATAAATATATGCTCTTCAATATAAAAAAAAAATATCAATAATTTTCAATATAGTAAAAATAATTAAAATATATATCCGAATACAGGAGGAGATAGGGGTGCTGGTACAGGCTCTTGCAAGGTCCGGCGAAGCTCCGGCGACCTTGGGGAGCTTGCTCACCGTGTGCACTGCGAATGTGCTTGGACGGACAATGATCAGCCGACGTGTTTTCGGTGATGGTAGCGGCGGCGATCCAGAGGCAAACATATTTAAGGCAATGGTGGAGCAAGTGATGGTGCTGCCCGGAAAGTTCAACCCCGGTGACTTTTTCCCGTCGCTTGAGTGGCTTGATTTGCTGGGTGTGGCAAGTGAGATGAAGAAGGTCCACAAATGGTTCGATGATTTTTTGACCAAGATCGTGGAGGAGCACCGGAATTTATTGGCCGGAGGCGGCGACCGAGGCGGCGACCAAGACTTACTGAGTACTCTTCTGTCATCGAAGGATGAAGCTGAAGATGAGAATGAGAAGCTTAATGACAGTGAGATCAAGGCTTTGTTGTTGGTATTATTTCTCTCACTACTCATTTTTTATTTTTCACTCTCCAAAAAAAAAAATGTTTTTGATTAAATTATTACAATATGCATAAACTTTTGCTAGTTATTTAAAAAATATCCCAATATTACTTTTGATTTTTTATAAACGATTCAAAACTATCATTTTTTTATTTTGTAGAAATCCATAGAATTTTATATAGAAATTAAGATTTGGAATGATGGGTTGTATGACCTAAAATCTAAAACAAAACTTCAGTTTGTCACATTGTTTTAGGTCACCATTACATAGTTACAAGTCTCAGTTTTTATCTAAAATTTGTGAAATTTTTTAGTTTTTATAATTAAGCCTATTTAAAAATTACTCTTAGACCGCGATATAATTCTATCGCGTTAGACTCTAAATTTTTTTTATATTTGTAAATTGTTTGATTCATTTTTCTATATATGAAAACAGAAAAAAAAAATGATTTAAAAGTAAAAAAGCTGTGTAGTTAATTTACATATATTAAAAGTTAATGTAACTTTGCCATTCTTATACATCGAATAAGAATCCATTTAAAAAAAGTAAAAAGAAAAACAGAAATGTAACAACAAAAAAAGTTATCTAAATGAGTTTTTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTTTTAAAAAAAAAAAAAAAAAAAAAAAAGAAAGAAAGAAAAAAAAGGAAAAAATTCATCCCTTCGTGCTAACAAAAACAAATGGAAAAGAGTAGATTGCCAAACCCTTTCATTGTAGAATAACAATCTTAATCAAATTCTTATGGTATTATTATTATTATTTCATGTAGAACATGTTTACAGCTGGAACCGACACGTCAGCTAGCACAGTGGAATGGGCCTTGACAGAGCTCATCAGACATCCCAAAATAATGGCCGAGGCCCAGAAAGAATTGGACACCGTAGTGGGCCGTGATCGCGCAGTTTCCGATCTGGACTTGTCGCAACTGCCCTATCTCCAGGCGGTGGTGAAGGAAACATTCCGGCTCCATCCACCGACGCCGCTCTCCGTCCCTCGCATGGCCGCCGAGAGCTGCGAAATCAACGGCTACCACATCCCCAAAGGCTCCACTCTCTTGGTCGACATATGGGCCATTAGTCGTGACCCAAAAGAATGGGCCGACCCACTCGAGTTCAGGCCCAATAGGTTCCTCCCAGGCGGCGAGAAGGCCCACGTGGATGTGAAGGGAAATGATTTTGAGGTGATTCCGTTTGGGGCTGGGCGGAGGATCTGCGTGGGTATGAGTATGGGCCTGATGATGGTCCAGATGTTAACTGCCACGCTGGCCCATTCTTTTGATTGGGCCTTGCCTAATGGGCTTTTGCCTGATAAGTTGAACATGGACGAGGCCTATGGGCTGACTCTGCGTCGGGCCCAACCCTTGGTTGTGCATCCACGGCCCAGATTATTGCCTGGGGTTTATCAAAAACTGGCTTCAATTTGTTAGATCTAGCATCTATTTCTTGGTTGTAAGTTTTCTTCTATTTGATTTGGACAAAATAGTAATAATAGTTTATAAATGAGTGTGCAAGATAAGTTTTCTTCTATTTGATTTGGACAAAATAGTAATAATAGTTTATAAATGAGTGTGCAAGATATAAGTAGTAGTTGAAGATTAATTTTTGCATGAATAAGTTGTTGTGAAACTCACATCGTCGTTGTACTCTAGAATTGTTAATAAAAGATTTAAATACTATTTTGATTCATGTACTTTTGATTTTTATTTATTTTGGTTCTTATACTTTTAAAAGTTTCATTTTGGTCTCTATACTTTCAACTTTGGTCCATTTTGGTCATGTACTTTTATAATGTTTATTTTGGTCCTTGAATTTTGAAAAAGTGACAATTTTTATCCTTTAAAAATGAAAATGAAGAGATCAAAATGACAAATTTTTTAAAAGTATAACATTAAAATGAACCAAAGTTAAAAGTACAATAACTAAAATGAACATTTTTAAAGTATAAGGACCAAAATGAACTAAAGTTGGAAGTATATGCATCAAAATAAATATTTCAAAGGTAGAAAGACTAAAGTGAATAAAAAGTAAAAGTATAGAGATTAAAGTGTTATTAGAAAAATAGGTAATAAATTGAAAGTAAATGAATCACGTTAGAGAGGAGAAAGGTATATAATTCCTCGGTAAATTTGAGCGCCAATCCAATCTAAGTCCATCAAAGTGCCTATTGCAGTACTTTCCGGCCGGCGGCAACTCATCGCCGGCGTCTTCCGCCTTCCGAGTTTTG

mRNA sequence

CCAAGACCAAGGATTCATCAAATAAATTTCGATCGTAGATCCTAACTACTTCAAAGTGGAAATTACAAATTTACCCATAAATCATCTCAACTCCATTTCCCTTTTAAGCACCGCCTTTCCTTCCCTTCCCTTCACTGAAATTCTCTATAACATATATATGGACGAAATTTCTCTCATCGTCGCCGCCGTTGTAGTAACCTTGGCCTACGCCATCCTCCTCCACCGCGCTTTCTTCCTCCGCCGCAGATTCTGCCTCCCGCCTGGTCCAAAACCATGGCCGATCGTAGGAAACATGCCGCAAATCGGCGGAGCGCCTCTCCACTCCCTCCTCGCCGCCATGGCCGGCGAGTACGGACCTCTGATGTACCTCCGATTAGGATCCGTTGACGTGGTCGTGGCGGCGTCGGCGTCGGTGGCGGAGCAACTCTTGAAGATTCACGGCACTAATTTCCTTAGCCGGCCGCCGAACGCCGGCGCAAAATATATTGCGTATAATAATCGGGATATGGTTTTTGCACCGTACGGTCCACGGTGGCGATTACTCCGGAAGATCAGCACCGTCCATCTGTTCTCCTCAAAAGCCCTAGATGATCATCGCCGCATACGGGAGGAGGAGATAGGGGTGCTGGTACAGGCTCTTGCAAGGTCCGGCGAAGCTCCGGCGACCTTGGGGAGCTTGCTCACCGTGTGCACTGCGAATGTGCTTGGACGGACAATGATCAGCCGACGTGTTTTCGGTGATGGTAGCGGCGGCGATCCAGAGGCAAACATATTTAAGGCAATGGTGGAGCAAGTGATGGTGCTGCCCGGAAAGTTCAACCCCGGTGACTTTTTCCCGTCGCTTGAGTGGCTTGATTTGCTGGGTGTGGCAAGTGAGATGAAGAAGGTCCACAAATGGTTCGATGATTTTTTGACCAAGATCGTGGAGGAGCACCGGAATTTATTGGCCGGAGGCGGCGACCGAGGCGGCGACCAAGACTTACTGAGTACTCTTCTGTCATCGAAGGATGAAGCTGAAGATGAGAATGAGAAGCTTAATGACAGTGAGATCAAGGCTTTGTTGTTGAACATGTTTACAGCTGGAACCGACACGTCAGCTAGCACAGTGGAATGGGCCTTGACAGAGCTCATCAGACATCCCAAAATAATGGCCGAGGCCCAGAAAGAATTGGACACCGTAGTGGGCCGTGATCGCGCAGTTTCCGATCTGGACTTGTCGCAACTGCCCTATCTCCAGGCGGTGGTGAAGGAAACATTCCGGCTCCATCCACCGACGCCGCTCTCCGTCCCTCGCATGGCCGCCGAGAGCTGCGAAATCAACGGCTACCACATCCCCAAAGGCTCCACTCTCTTGGTCGACATATGGGCCATTAGTCGTGACCCAAAAGAATGGGCCGACCCACTCGAGTTCAGGCCCAATAGGTTCCTCCCAGGCGGCGAGAAGGCCCACGTGGATGTGAAGGGAAATGATTTTGAGGTGATTCCGTTTGGGGCTGGGCGGAGGATCTGCGTGGGTATGAGTATGGGCCTGATGATGGTCCAGATGTTAACTGCCACGCTGGCCCATTCTTTTGATTGGGCCTTGCCTAATGGGCTTTTGCCTGATAAGTTGAACATGGACGAGGCCTATGGGCTGACTCTGCGTCGGGCCCAACCCTTGGTTGTGCATCCACGGCCCAGATTATTGCCTGGGTACTTTCCGGCCGGCGGCAACTCATCGCCGGCGTCTTCCGCCTTCCGAGTTTTG

Coding sequence (CDS)

ATGGACGAAATTTCTCTCATCGTCGCCGCCGTTGTAGTAACCTTGGCCTACGCCATCCTCCTCCACCGCGCTTTCTTCCTCCGCCGCAGATTCTGCCTCCCGCCTGGTCCAAAACCATGGCCGATCGTAGGAAACATGCCGCAAATCGGCGGAGCGCCTCTCCACTCCCTCCTCGCCGCCATGGCCGGCGAGTACGGACCTCTGATGTACCTCCGATTAGGATCCGTTGACGTGGTCGTGGCGGCGTCGGCGTCGGTGGCGGAGCAACTCTTGAAGATTCACGGCACTAATTTCCTTAGCCGGCCGCCGAACGCCGGCGCAAAATATATTGCGTATAATAATCGGGATATGGTTTTTGCACCGTACGGTCCACGGTGGCGATTACTCCGGAAGATCAGCACCGTCCATCTGTTCTCCTCAAAAGCCCTAGATGATCATCGCCGCATACGGGAGGAGGAGATAGGGGTGCTGGTACAGGCTCTTGCAAGGTCCGGCGAAGCTCCGGCGACCTTGGGGAGCTTGCTCACCGTGTGCACTGCGAATGTGCTTGGACGGACAATGATCAGCCGACGTGTTTTCGGTGATGGTAGCGGCGGCGATCCAGAGGCAAACATATTTAAGGCAATGGTGGAGCAAGTGATGGTGCTGCCCGGAAAGTTCAACCCCGGTGACTTTTTCCCGTCGCTTGAGTGGCTTGATTTGCTGGGTGTGGCAAGTGAGATGAAGAAGGTCCACAAATGGTTCGATGATTTTTTGACCAAGATCGTGGAGGAGCACCGGAATTTATTGGCCGGAGGCGGCGACCGAGGCGGCGACCAAGACTTACTGAGTACTCTTCTGTCATCGAAGGATGAAGCTGAAGATGAGAATGAGAAGCTTAATGACAGTGAGATCAAGGCTTTGTTGTTGAACATGTTTACAGCTGGAACCGACACGTCAGCTAGCACAGTGGAATGGGCCTTGACAGAGCTCATCAGACATCCCAAAATAATGGCCGAGGCCCAGAAAGAATTGGACACCGTAGTGGGCCGTGATCGCGCAGTTTCCGATCTGGACTTGTCGCAACTGCCCTATCTCCAGGCGGTGGTGAAGGAAACATTCCGGCTCCATCCACCGACGCCGCTCTCCGTCCCTCGCATGGCCGCCGAGAGCTGCGAAATCAACGGCTACCACATCCCCAAAGGCTCCACTCTCTTGGTCGACATATGGGCCATTAGTCGTGACCCAAAAGAATGGGCCGACCCACTCGAGTTCAGGCCCAATAGGTTCCTCCCAGGCGGCGAGAAGGCCCACGTGGATGTGAAGGGAAATGATTTTGAGGTGATTCCGTTTGGGGCTGGGCGGAGGATCTGCGTGGGTATGAGTATGGGCCTGATGATGGTCCAGATGTTAACTGCCACGCTGGCCCATTCTTTTGATTGGGCCTTGCCTAATGGGCTTTTGCCTGATAAGTTGAACATGGACGAGGCCTATGGGCTGACTCTGCGTCGGGCCCAACCCTTGGTTGTGCATCCACGGCCCAGATTATTGCCTGGGTACTTTCCGGCCGGCGGCAACTCATCGCCGGCGTCTTCCGCCTTCCGAGTTTTG

Protein sequence

MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTANVLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWALPNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGYFPAGGNSSPASSAFRVL
Homology
[Close]
BLAST of Lsi06G014210 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 8.7e-189
Identity = 326/511 (63.80%), Postives = 404/511 (79.06%), Query Frame = 0 

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           M+ +SLI+  V+ +     +L   F  R    LPPGPKPWPI+GN+  +G  P H   AA
Sbjct: 1   MEILSLILYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKP-HQSTAA 60

Query: 61  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
           MA  YGPLMYL++G VDVVVAASASVA Q LK H  NF SRPPN+GA+++AYN +D+VFA
Sbjct: 61  MAQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFA 120

Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWR+LRKI +VHLFS+KALDD R +R++E+  L +ALA +G+ P  LG LL VCT 
Sbjct: 121 PYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTT 180

Query: 181 NVLGRTMISRRVFGDGSGG-DPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
           N L R M+ +RVF DGSG  DP+A  FK+MV ++MV+ G FN GDF P L WLD+ GVA+
Sbjct: 181 NALARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAA 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSK-DEAEDENEKLNDSEI 300
           +MKK+H  FD FLT I+EEH+     G   G  +DLLSTL+S K D+A+++  KL D+EI
Sbjct: 241 KMKKLHARFDAFLTDILEEHK-----GKIFGEMKDLLSTLISLKNDDADNDGGKLTDTEI 300

Query: 301 KALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPY 360
           KALLLN+F AGTDTS+STVEWA+ ELIR+PKI+A+AQ+E+D VVGRDR V +LDL+QL Y
Sbjct: 301 KALLLNLFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTY 360

Query: 361 LQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEF 420
           L+A+VKETFRLHP TPLS+PR+A+ESCEINGY IPKGSTLL+++WAI+RDP  WADPLEF
Sbjct: 361 LEAIVKETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEF 420

Query: 421 RPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWALPN 480
           RP RFLPGGEK  VDV+GNDFEVIPFGAGRRIC GM++G+ MVQ++ ATL H+F+W L +
Sbjct: 421 RPERFLPGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVS 480

Query: 481 GLLPDKLNMDEAYGLTLRRAQPLVVHPRPRL 510
           G LP+ LNM+EAYGLTL+RA PLVVHPRPRL
Sbjct: 481 GQLPEMLNMEEAYGLTLQRADPLVVHPRPRL 505

[Close]
BLAST of Lsi06G014210 vs. ExPASy Swiss-Prot
Match: Q9SD85 (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 7.9e-182
Identity = 316/518 (61.00%), Postives = 395/518 (76.25%), Query Frame = 0 

Query: 5   SLIVAAVVVTLAYAILLHRAFFLRRRFC----LPPGPKPWPIVGNMPQIGGAPLHSLLAA 64
           +L +  ++ T+ + IL  R F  RR       LPPGP PWPI+GN+P +G  P H  L+A
Sbjct: 3   TLFLTILLATVLFLIL--RIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSA 62

Query: 65  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 124
           M   YGP+++LRLG VDVVVAAS SVAEQ LKIH  NF SRPPN+GAK++AYN +D+VFA
Sbjct: 63  MVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFA 122

Query: 125 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 184
           PYG RWRLLRKIS+VHLFS+KAL+D + +R+EE+G L + L R G  P  LG L+ +C  
Sbjct: 123 PYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVV 182

Query: 185 NVLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASE 244
           N LGR MI RR+F  G+  D +A+ F++MV ++M L G FN GDF PSL+WLDL GVA +
Sbjct: 183 NALGREMIGRRLF--GADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGK 242

Query: 245 MKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSK-DEAEDENEKLNDSEIK 304
           MK++HK FD FL+ I++EH       G      D+LSTL+S K  + + +   L D+EIK
Sbjct: 243 MKRLHKRFDAFLSSILKEHEM----NGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIK 302

Query: 305 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 364
           ALLLNMFTAGTDTSASTV+WA+ ELIRHP IM +AQ+ELD VVGRDR V++ D++QLPYL
Sbjct: 303 ALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYL 362

Query: 365 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 424
           QAV+KE FRLHPPTPLS+P +A+ESCEINGYHIPKGSTLL +IWAI+RDP +W+DPL F+
Sbjct: 363 QAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFK 422

Query: 425 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWALPNG 484
           P RFLPGGEK+ VDVKG+DFE+IPFGAGRRIC G+S+GL  +Q LTATL   FDW L  G
Sbjct: 423 PERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGG 482

Query: 485 LLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGYFPAG 518
           + P+KLNM+E+YGLTL+RA PLVVHP+PRL P  +  G
Sbjct: 483 VTPEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVYGLG 511

[Close]
BLAST of Lsi06G014210 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 8.8e-165
Identity = 291/511 (56.95%), Postives = 371/511 (72.60%), Query Frame = 0 

Query: 5   SLIVAAVVVTLAYAILLHRAFFLRRRFC----LPPGPKPWPIVGNMPQIGGAPLHSLLAA 64
           +L++++ +V L Y          RRR      LPPGP  WPI+GN+PQ+G  P H  L A
Sbjct: 11  TLLLSSFIVYLLY----------RRRSAQCPPLPPGPNGWPILGNLPQLGAKP-HQTLDA 70

Query: 65  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 124
           ++ +YGPL  LRLGSV+VVVA+S++VA Q L+ H  NF +RPPN+GA+++AYN +D+VFA
Sbjct: 71  LSKQYGPLFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFA 130

Query: 125 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAP--ATLGSLLTVC 184
           PYGPRWR+LRK+ +VHLFS KALDD R +R+ E+  LV+ L R  +      LG  L VC
Sbjct: 131 PYGPRWRMLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVC 190

Query: 185 TANVLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVA 244
             N L R M+ RRVF D      EA+ FK MV ++M L G FN GDF P L WLDL GV 
Sbjct: 191 ATNALARAMLGRRVFADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVV 250

Query: 245 SEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEI 304
            +MK++H+ +D FL +++EE++     G       DLLS L+  K+   +   KLN+++I
Sbjct: 251 GKMKRLHRRYDAFLDRVIEENQANAKSG-------DLLSVLIRLKEADAEGEIKLNNTDI 310

Query: 305 KALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPY 364
           KALLLN+FTAGTDTS+STVEW L ELIRHP I+ + Q ELD+V+GRDR V++ DL  LPY
Sbjct: 311 KALLLNLFTAGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPY 370

Query: 365 LQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEF 424
           LQAVVKETFRLHP TPLS+PRMA+E C +NGY IPK +TLLV++W+I RD   W DPLEF
Sbjct: 371 LQAVVKETFRLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEF 430

Query: 425 RPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWALPN 484
           RP+RFLPGGE+ HVDVKGNDFEVIPFGAGRRIC G+S+GL MVQ +TAT+ H++DW+LP 
Sbjct: 431 RPSRFLPGGEREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPK 490

Query: 485 GLLPDKLNMDEAYGLTLRRAQPLVVHPRPRL 510
           G    KL+M+EAYGLTL+RA PL+V P PRL
Sbjct: 491 GQECQKLDMEEAYGLTLQRAVPLMVQPIPRL 503

[Close]
BLAST of Lsi06G014210 vs. ExPASy Swiss-Prot
Match: Q7G602 (Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B3 PE=2 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 1.5e-161
Identity = 282/493 (57.20%), Postives = 357/493 (72.41%), Query Frame = 0 

Query: 28  RRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGEYGPLMYLRLGSVDVVVAASASVA 87
           R+R  LPPGP+ WP++GN+PQ+G  P H  + A+A +YGPL  LR G  +VVVAASA VA
Sbjct: 36  RKRRPLPPGPRGWPVLGNLPQLGDKP-HHTMCALARQYGPLFRLRFGCAEVVVAASAPVA 95

Query: 88  EQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAPYGPRWRLLRKISTVHLFSSKALDDHR 147
            Q L+ H  NF +RPPN+GA+++AYN +D+VFAPYG RWR LRK+  +HLFS+KALDD R
Sbjct: 96  AQFLRGHDANFSNRPPNSGAEHVAYNYQDLVFAPYGARWRALRKLCALHLFSAKALDDLR 155

Query: 148 RIREEEIGVLVQALARSGEAPATLGSLLTVCTANVLGRTMISRRVFG-DGSGGDPEANIF 207
            +RE E+ ++V+ LAR   A   LG    VC  N L R  I  RVF  DG  G   A  F
Sbjct: 156 AVREGEVALMVRNLARQQAASVALGQEANVCATNTLARATIGHRVFAVDGGEG---AREF 215

Query: 208 KAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASEMKKVHKWFDDFLTKIVEEHRNLLAGG 267
           K MV ++M L G FN GDF P+L WLD  GV ++MK++H+ +D+ +   + E +      
Sbjct: 216 KEMVVELMQLAGVFNVGDFVPALRWLDPQGVVAKMKRLHRRYDNMMNGFINERKAGAQPD 275

Query: 268 GDRGGD--QDLLSTLLSSKDE---AEDENEKLNDSEIKALLLNMFTAGTDTSASTVEWAL 327
           G   G+   DLLS LL+   E    + + EK+ +++IKALLLN+FTAGTDT++STVEWAL
Sbjct: 276 GVAAGEHGNDLLSVLLARMQEEQKLDGDGEKITETDIKALLLNLFTAGTDTTSSTVEWAL 335

Query: 328 TELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYLQAVVKETFRLHPPTPLSVPRMA 387
            ELIRHP ++ EAQ ELDTVVGR R VS+ DL +LPYL AV+KETFRLHP TPLS+PR A
Sbjct: 336 AELIRHPDVLKEAQHELDTVVGRGRLVSESDLPRLPYLTAVIKETFRLHPSTPLSLPREA 395

Query: 388 AESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEV 447
           AE CE++GY IPKG+TLLV++WAI+RDP +W DPL+++P+RFLPG   A VDVKG DF +
Sbjct: 396 AEECEVDGYRIPKGATLLVNVWAIARDPTQWPDPLQYQPSRFLPGRMHADVDVKGADFGL 455

Query: 448 IPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWALPNGLLPDKLNMDEAYGLTLRRAQPL 507
           IPFGAGRRIC G+S GL MV ++TATL H FDW L NG  PDKLNM+EAYGLTL+RA PL
Sbjct: 456 IPFGAGRRICAGLSWGLRMVTLMTATLVHGFDWTLANGATPDKLNMEEAYGLTLQRAVPL 515

Query: 508 VVHPRPRLLPGYF 515
           +V P PRLLP  +
Sbjct: 516 MVQPVPRLLPSAY 524

[Close]
BLAST of Lsi06G014210 vs. ExPASy Swiss-Prot
Match: Q8LM92 (Flavonoid 3'-monooxygenase CYP75B4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B4 PE=2 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 3.9e-157
Identity = 283/522 (54.21%), Postives = 358/522 (68.58%), Query Frame = 0 

Query: 6   LIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGEY 65
           L   A+ V + YA++  RA   R    LPPGP+ WP++GN+PQ+GG   H  L  M   Y
Sbjct: 14  LTTVALSVIVCYALVFSRAGKARAPLPLPPGPRGWPVLGNLPQLGG-KTHQTLHEMTKVY 73

Query: 66  GPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAPYGPR 125
           GPL+ LR GS DVVVA SA VA Q L+ H  NF SRP N+G +++AYN RD+VF PYGPR
Sbjct: 74  GPLIRLRFGSSDVVVAGSAPVAAQFLRTHDANFSSRPRNSGGEHMAYNGRDVVFGPYGPR 133

Query: 126 WRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPAT-------LGSLLTVC 185
           WR +RKI  V+LFS++ALDD R  RE E  ++V++LA +  AP +       LG  + VC
Sbjct: 134 WRAMRKICAVNLFSARALDDLRAFREREAVLMVRSLAEASAAPGSSSPAAVVLGKEVNVC 193

Query: 186 TANVLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVA 245
           T N L R  + RRVF  G+G    A  FK +V +VM + G  N GDF P+L WLD  GV 
Sbjct: 194 TTNALSRAAVGRRVFAAGAG--EGAREFKEIVLEVMEVGGVLNVGDFVPALRWLDPQGVV 253

Query: 246 SEMKKVHKWFDDFLTKIVEEHR--NLLAGGGDRGGDQDLLSTLLSSKDEAE----DENEK 305
           + MKK+H+ FDD +  I+ E R  +LL     R   +DLL  LL+   E E     E+++
Sbjct: 254 ARMKKLHRRFDDMMNAIIAERRAGSLLKPTDSREEGKDLLGLLLAMVQEQEWLAAGEDDR 313

Query: 306 LNDSEIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLD 365
           + D+EIKAL+LN+F AGTDT+++ VEW + ELIRHP I+  AQ+ELD VVGRDR +S+ D
Sbjct: 314 ITDTEIKALILNLFVAGTDTTSTIVEWTMAELIRHPDILKHAQEELDVVVGRDRLLSESD 373

Query: 366 LSQLPYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEW 425
           LS L +  A++KETFRLHP TPLS+PRMA+E CEI GY IPKG+ LLV++W I+RDP  W
Sbjct: 374 LSHLTFFHAIIKETFRLHPSTPLSLPRMASEECEIAGYRIPKGAELLVNVWGIARDPAIW 433

Query: 426 ADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSF 485
            DPLE++P+RFLPGG    VDVKGNDF +IPFGAGRRIC G+S GL MV M  ATL H+F
Sbjct: 434 PDPLEYKPSRFLPGGTHTDVDVKGNDFGLIPFGAGRRICAGLSWGLRMVTMTAATLVHAF 493

Query: 486 DWALPNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGYF 515
           DW LP    PDKLNMDEA+ L L+RA+PLVVHP PRLLP  +
Sbjct: 494 DWQLPADQTPDKLNMDEAFTLLLQRAEPLVVHPVPRLLPSAY 532

[Close]
BLAST of Lsi06G014210 vs. ExPASy TrEMBL
Match: A0A5A7UTD5 (Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002910 PE=3 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 4.3e-255
Identity = 445/518 (85.91%), Postives = 473/518 (91.31%), Query Frame = 0 

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           M+EI+LIVAA ++TLA A LLHR FFLRRRF LPPGPKPWPIVGNMPQIGGAPLHSLLAA
Sbjct: 1   MEEITLIVAA-IITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60

Query: 61  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
           MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120

Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWRLLRKIS VHLFSSKALDDHRRIR++EI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 180

Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
           N LGRTMI RRVFGDGSG D  E+N FKAMVEQVMVL GKFNPGDFFP LE LDL+GV S
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLA---GGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
           EMKKVHKWFDDFLTKIVEEHRNLLA   GG   GG QDLLSTLLS KDE  DENEKLND+
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDT 300

Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
           EIKALLLNMFTAGTDTSASTVEWALTELIRHP++MA+AQ+ELD+VVGRDRAVSDLDL QL
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQL 360

Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
            YLQAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPKEW DPL
Sbjct: 361 VYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPL 420

Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWAL 480
           EF PNRFLP GEK HVDVKGNDFE IPFGAGRRICVG+S+GL MVQMLTAT+ HSFDWAL
Sbjct: 421 EFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWAL 480

Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGYF 515
           PNGL PDKLNMDE YGLTLRRAQPLVVHPRPRLL G +
Sbjct: 481 PNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVY 517

[Close]
BLAST of Lsi06G014210 vs. ExPASy TrEMBL
Match: A0A1S3BVU8 (flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 4.3e-255
Identity = 445/518 (85.91%), Postives = 473/518 (91.31%), Query Frame = 0 

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           M+EI+LIVAA ++TLA A LLHR FFLRRRF LPPGPKPWPIVGNMPQIGGAPLHSLLAA
Sbjct: 1   MEEITLIVAA-IITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60

Query: 61  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
           MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120

Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWRLLRKIS VHLFSSKALDDHRRIR++EI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 180

Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
           N LGRTMI RRVFGDGSG D  E+N FKAMVEQVMVL GKFNPGDFFP LE LDL+GV S
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLA---GGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
           EMKKVHKWFDDFLTKIVEEHRNLLA   GG   GG QDLLSTLLS KDE  DENEKLND+
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDT 300

Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
           EIKALLLNMFTAGTDTSASTVEWALTELIRHP++MA+AQ+ELD+VVGRDRAVSDLDL QL
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQL 360

Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
            YLQAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPKEW DPL
Sbjct: 361 VYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPL 420

Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWAL 480
           EF PNRFLP GEK HVDVKGNDFE IPFGAGRRICVG+S+GL MVQMLTAT+ HSFDWAL
Sbjct: 421 EFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWAL 480

Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGYF 515
           PNGL PDKLNMDE YGLTLRRAQPLVVHPRPRLL G +
Sbjct: 481 PNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVY 517

[Close]
BLAST of Lsi06G014210 vs. ExPASy TrEMBL
Match: A0A6J1EZJ7 (flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 2.3e-245
Identity = 428/518 (82.63%), Postives = 465/518 (89.77%), Query Frame = 0 

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRR---FCLPPGPKPWPIVGNMPQIGGAPLHSL 60
           MDEISL  +A  +TLA  +LL+R  F +R      LPPGPKPWPIVGN+PQIGGAPLHSL
Sbjct: 1   MDEISLFFSA-AITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60

Query: 61  LAAMAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDM 120
           LA M+GEYGPLMYLR GSVDVVVAASA++AEQ LKI  TNFLSRPPNAGAKYIAYN +DM
Sbjct: 61  LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTV 180
           VFAPYGPRWRLLRKIS VHLFSSKAL+DHRR+REEE+GVLVQALARSGEAPATLGSLLTV
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180

Query: 181 CTANVLGRTMISRRVFGDGS-GGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLG 240
           CTAN LGRTMI RRVFGDGS GGDP ++ FKAMVEQVM+L GKFNPGDFF  LEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLG 240

Query: 241 VASEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
           VA EMKKVHKWFDD ++KI+EEHRNLLA GG  G  QDLLSTLLS++D A+DENEKLND+
Sbjct: 241 VAGEMKKVHKWFDDLMSKILEEHRNLLATGGG-GEHQDLLSTLLSTRDRAKDENEKLNDT 300

Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
           EIKALLLNMFTAGTDTSASTVEWAL ELIRHPK+M +AQ+ELD+VVGRDR VSDLDL +L
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKL 360

Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
            YLQAVVKETFRLHPPTPLS+PRMAAESCEI+GYHIPKGSTLLVD+WAISRDPKEW  PL
Sbjct: 361 GYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPL 420

Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWAL 480
           EFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMSMGL MVQMLTA+L HSFDWAL
Sbjct: 421 EFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWAL 480

Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGYF 515
           P+GL P +LNMDEA+GLTLRRAQPLV HPRPRL PG +
Sbjct: 481 PSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVY 516

[Close]
BLAST of Lsi06G014210 vs. ExPASy TrEMBL
Match: A0A6J1L0D6 (flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 7.5e-244
Identity = 425/518 (82.05%), Postives = 463/518 (89.38%), Query Frame = 0 

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRR---FCLPPGPKPWPIVGNMPQIGGAPLHSL 60
           MDEISL  +A  +TLA  +LL+R  F +R      LPPGPKPWP VGN+PQIGGAPLHS 
Sbjct: 1   MDEISLFFSA-AITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQ 60

Query: 61  LAAMAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDM 120
           LAAM+GEYGPLMYLR GS+DVVVAASA++AEQ LKI  TNFLSRPPNAGAKYIAYN +DM
Sbjct: 61  LAAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTV 180
           VFAPYGPRWRLLRKIS VHLFSSKAL+D+R +REEE+GVLVQALAR GEAPATLGSLLTV
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTV 180

Query: 181 CTANVLGRTMISRRVFGDGS-GGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLG 240
           CTAN LGRTMI RRVFGDGS GGDP ++ FKAMVEQVM+L GKFNPG+FFPSLEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLG 240

Query: 241 VASEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
           VA EMKKVHKWFDD L+KI+EEHR LLA GG+    QDLLSTLLS++D AEDENEKLND+
Sbjct: 241 VAREMKKVHKWFDDLLSKILEEHRTLLASGGEH---QDLLSTLLSTRDHAEDENEKLNDT 300

Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
           EIKALLLNMFTAGTDTSASTVEWAL ELIRHPK+M +AQ+ELD+VVGRDR VSDLDL +L
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKL 360

Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
            YLQAVVKETFRLHPPTPLS+PRMAAESCEINGYHIPKGSTLLVD+WAISRDPKEW  PL
Sbjct: 361 GYLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPL 420

Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWAL 480
           EFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMSMGL MVQMLTA+L HSFDWAL
Sbjct: 421 EFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWAL 480

Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGYF 515
           P+GL P +LNMDEA+GLTLRRAQPLV HPRPRL PG +
Sbjct: 481 PSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVY 514

[Close]
BLAST of Lsi06G014210 vs. ExPASy TrEMBL
Match: A0A0A0LP99 (Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 1.9e-226
Identity = 403/508 (79.33%), Postives = 433/508 (85.24%), Query Frame = 0 

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           M+EI+ IVAA ++TLA A LLHRAFFLRRR  LPPGPKPWPIVGNMPQIG A LHSLLA 
Sbjct: 1   MEEITPIVAA-LITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLAT 60

Query: 61  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
           MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120

Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIA 180

Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
           N LGRTMI RRVFGDGSG D  E+N FK MVEQVMVL GKFNPGDFFP LEWLDL+GV  
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGR 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIK 300
           EMKKVHKWFDDFLTKIVEEHRNLLA G   GG QDLLSTLLS KD+ EDENEKLND+EIK
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGVG-GGHQDLLSTLLSMKDDGEDENEKLNDTEIK 300

Query: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 360
           ALLL +F                           ++  L+  +GRDRAVSD+DL QL YL
Sbjct: 301 ALLLVLFL--------------------------SKFYLNCFIGRDRAVSDVDLHQLVYL 360

Query: 361 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 420
           QAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPK+W DPLEFR
Sbjct: 361 QAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFR 420

Query: 421 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWALPNG 480
           PNRFLP GEK HVDVKGNDFEVIPFGAGRRICVG+S+GL MVQMLTAT+ HSFDW LPNG
Sbjct: 421 PNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNG 480

Query: 481 LLPDKLNMDEAYGLTLRRAQPLVVHPRP 508
           L PDKLNMDE YGLTLRRAQPL++HPRP
Sbjct: 481 LTPDKLNMDEHYGLTLRRAQPLIMHPRP 480

[Close]
BLAST of Lsi06G014210 vs. NCBI nr
Match: XP_038878698.1 (flavonoid 3'-monooxygenase [Benincasa hispida])

HSP 1 Score: 909.1 bits (2348), Expect = 1.8e-260
Identity = 460/515 (89.32%), Postives = 477/515 (92.62%), Query Frame = 0 

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           MDEI+LIVAA VVTLA  ILLHR FF R RF LPPGPKPWPIVGNMPQIGGAPLHSLLAA
Sbjct: 1   MDEIALIVAA-VVTLASTILLHRTFFRRSRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60

Query: 61  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
           MAGEYG LMYLRLGSV VVVAASA+VAE+LLK+H TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61  MAGEYGSLMYLRLGSVGVVVAASAAVAEKLLKVHDTNFLSRPPNAGAKYIAYNYQDMVFA 120

Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEI VLVQALA SGE PA LGSLLTVCTA
Sbjct: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIAVLVQALASSGEVPAALGSLLTVCTA 180

Query: 181 NVLGRTMISRRVFGD-GSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
           N LGRTMI RRVFGD GSG D EA  FKAMVEQVM+L GKFNPGDFFPSLEWLDLLGVAS
Sbjct: 181 NALGRTMIGRRVFGDGGSGDDSEAKQFKAMVEQVMILAGKFNPGDFFPSLEWLDLLGVAS 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIK 300
           EMKKVHKWFDDFLTKIVEEHRNLLA G   GG QDLLSTLLSS   AEDENEK+ND+EIK
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGRG-GGHQDLLSTLLSS---AEDENEKINDTEIK 300

Query: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 360
           ALLLNMFTAGTDTSASTVEWALTELIRHPK+MA+AQ+ELD+VVGRDRAVSDLDL QL YL
Sbjct: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSVVGRDRAVSDLDLPQLVYL 360

Query: 361 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 420
           QAVVKETFRLHPPTPLS+PRMAAESCEINGYHIPKG+TLLVD+WAI RDPKEWADPLEFR
Sbjct: 361 QAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGTTLLVDVWAIGRDPKEWADPLEFR 420

Query: 421 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWALPNG 480
           PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGL MVQMLTATL H FDWALPNG
Sbjct: 421 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLVHYFDWALPNG 480

Query: 481 LLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGYF 515
           LLPD+LNMDEAYGLTLRRAQPLVVHPRPRLLPG +
Sbjct: 481 LLPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 510

[Close]
BLAST of Lsi06G014210 vs. NCBI nr
Match: XP_008453263.1 (PREDICTED: flavonoid 3'-monooxygenase [Cucumis melo] >KAA0057987.1 flavonoid 3'-monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 890.2 bits (2299), Expect = 8.8e-255
Identity = 445/518 (85.91%), Postives = 473/518 (91.31%), Query Frame = 0 

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           M+EI+LIVAA ++TLA A LLHR FFLRRRF LPPGPKPWPIVGNMPQIGGAPLHSLLAA
Sbjct: 1   MEEITLIVAA-IITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60

Query: 61  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
           MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120

Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWRLLRKIS VHLFSSKALDDHRRIR++EI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 180

Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
           N LGRTMI RRVFGDGSG D  E+N FKAMVEQVMVL GKFNPGDFFP LE LDL+GV S
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLA---GGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
           EMKKVHKWFDDFLTKIVEEHRNLLA   GG   GG QDLLSTLLS KDE  DENEKLND+
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDT 300

Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
           EIKALLLNMFTAGTDTSASTVEWALTELIRHP++MA+AQ+ELD+VVGRDRAVSDLDL QL
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQL 360

Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
            YLQAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPKEW DPL
Sbjct: 361 VYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPL 420

Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWAL 480
           EF PNRFLP GEK HVDVKGNDFE IPFGAGRRICVG+S+GL MVQMLTAT+ HSFDWAL
Sbjct: 421 EFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWAL 480

Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGYF 515
           PNGL PDKLNMDE YGLTLRRAQPLVVHPRPRLL G +
Sbjct: 481 PNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVY 517

[Close]
BLAST of Lsi06G014210 vs. NCBI nr
Match: XP_004138192.1 (flavonoid 3'-monooxygenase [Cucumis sativus] >KGN63725.2 hypothetical protein Csa_014030 [Cucumis sativus])

HSP 1 Score: 881.3 bits (2276), Expect = 4.1e-252
Identity = 438/515 (85.05%), Postives = 470/515 (91.26%), Query Frame = 0 

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           M+EI+ IVAA ++TLA A LLHRAFFLRRR  LPPGPKPWPIVGNMPQIG A LHSLLA 
Sbjct: 1   MEEITPIVAA-LITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLAT 60

Query: 61  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
           MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120

Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIA 180

Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
           N LGRTMI RRVFGDGSG D  E+N FK MVEQVMVL GKFNPGDFFP LEWLDL+GV  
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGR 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIK 300
           EMKKVHKWFDDFLTKIVEEHRNLLA G   GG QDLLSTLLS KD+ EDENEKLND+EIK
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGVG-GGHQDLLSTLLSMKDDGEDENEKLNDTEIK 300

Query: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 360
           ALLLNMFTAGTDTSASTVEWALTELIRHP++MA+AQ+ELD++VGRDRAVSD+DL QL YL
Sbjct: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYL 360

Query: 361 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 420
           QAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPK+W DPLEFR
Sbjct: 361 QAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFR 420

Query: 421 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWALPNG 480
           PNRFLP GEK HVDVKGNDFEVIPFGAGRRICVG+S+GL MVQMLTAT+ HSFDW LPNG
Sbjct: 421 PNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNG 480

Query: 481 LLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGYF 515
           L PDKLNMDE YGLTLRRAQPL++HPRPRLL G +
Sbjct: 481 LTPDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVY 513

[Close]
BLAST of Lsi06G014210 vs. NCBI nr
Match: XP_023529494.1 (flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo] >XP_023529495.1 flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 862.1 bits (2226), Expect = 2.6e-246
Identity = 429/520 (82.50%), Postives = 467/520 (89.81%), Query Frame = 0 

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRR---FCLPPGPKPWPIVGNMPQIGGAPLHSL 60
           MDEISL  +A  +TLA  +LL+R  F +R      LPPGPKPWPIVGN+PQIGGAPLHSL
Sbjct: 1   MDEISLFFSA-AITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60

Query: 61  LAAMAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDM 120
           LA M+GEYGPLMYLR GSVDVVVAASA++AEQ LKI  TNFLSRPPNAGAKYIAYN +DM
Sbjct: 61  LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTV 180
           VFAPYGPRWRLLRKIS VHLFSSKAL+DHRR+REEE+GVLVQALARSGEAPATLGSLLTV
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180

Query: 181 CTANVLGRTMISRRVFGDGS-GGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLG 240
           CTAN LGRTMI RRVFGDGS GGDP ++ FKAMVEQVM+L GKFNPGDFFPSL+WLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFPSLDWLDLLG 240

Query: 241 VASEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGG--DQDLLSTLLSSKDEAEDENEKLN 300
           VA EMKKVHKWFDD L+KI+EEHRNLLA GG  GG   QDLLSTLLS++D AEDENEKLN
Sbjct: 241 VAREMKKVHKWFDDLLSKILEEHRNLLASGGGGGGGEHQDLLSTLLSARDRAEDENEKLN 300

Query: 301 DSEIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLS 360
           D+EIKALLLNMFTAGTDTSASTVEWAL ELIRHPK+M +AQ+ELD++VGRDR VSDLDL 
Sbjct: 301 DTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMHQAQQELDSIVGRDRVVSDLDLP 360

Query: 361 QLPYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWAD 420
           +L YLQAVVKETFRLHPPTPLS+PRMAAESCEI+GY IPKGSTLLVD+WAISRDPKEW  
Sbjct: 361 KLGYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYRIPKGSTLLVDVWAISRDPKEWTS 420

Query: 421 PLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDW 480
           PLEFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMSMGL MVQ+LTA+L HSFDW
Sbjct: 421 PLEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQILTASLVHSFDW 480

Query: 481 ALPNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGYF 515
           ALP+GL P +LNMDEA+GLTLRRAQPLV HPRPRL PG +
Sbjct: 481 ALPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVY 519

[Close]
BLAST of Lsi06G014210 vs. NCBI nr
Match: XP_022933611.1 (flavonoid 3'-monooxygenase [Cucurbita moschata])

HSP 1 Score: 857.8 bits (2215), Expect = 4.8e-245
Identity = 428/518 (82.63%), Postives = 465/518 (89.77%), Query Frame = 0 

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRR---FCLPPGPKPWPIVGNMPQIGGAPLHSL 60
           MDEISL  +A  +TLA  +LL+R  F +R      LPPGPKPWPIVGN+PQIGGAPLHSL
Sbjct: 1   MDEISLFFSA-AITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60

Query: 61  LAAMAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDM 120
           LA M+GEYGPLMYLR GSVDVVVAASA++AEQ LKI  TNFLSRPPNAGAKYIAYN +DM
Sbjct: 61  LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTV 180
           VFAPYGPRWRLLRKIS VHLFSSKAL+DHRR+REEE+GVLVQALARSGEAPATLGSLLTV
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180

Query: 181 CTANVLGRTMISRRVFGDGS-GGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLG 240
           CTAN LGRTMI RRVFGDGS GGDP ++ FKAMVEQVM+L GKFNPGDFF  LEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLG 240

Query: 241 VASEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
           VA EMKKVHKWFDD ++KI+EEHRNLLA GG  G  QDLLSTLLS++D A+DENEKLND+
Sbjct: 241 VAGEMKKVHKWFDDLMSKILEEHRNLLATGGG-GEHQDLLSTLLSTRDRAKDENEKLNDT 300

Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
           EIKALLLNMFTAGTDTSASTVEWAL ELIRHPK+M +AQ+ELD+VVGRDR VSDLDL +L
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKL 360

Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
            YLQAVVKETFRLHPPTPLS+PRMAAESCEI+GYHIPKGSTLLVD+WAISRDPKEW  PL
Sbjct: 361 GYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPL 420

Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWAL 480
           EFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMSMGL MVQMLTA+L HSFDWAL
Sbjct: 421 EFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWAL 480

Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGYF 515
           P+GL P +LNMDEA+GLTLRRAQPLV HPRPRL PG +
Sbjct: 481 PSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVY 516

[Close]
BLAST of Lsi06G014210 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 638.3 bits (1645), Expect = 5.6e-183
Identity = 316/518 (61.00%), Postives = 395/518 (76.25%), Query Frame = 0 

Query: 5   SLIVAAVVVTLAYAILLHRAFFLRRRFC----LPPGPKPWPIVGNMPQIGGAPLHSLLAA 64
           +L +  ++ T+ + IL  R F  RR       LPPGP PWPI+GN+P +G  P H  L+A
Sbjct: 3   TLFLTILLATVLFLIL--RIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSA 62

Query: 65  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 124
           M   YGP+++LRLG VDVVVAAS SVAEQ LKIH  NF SRPPN+GAK++AYN +D+VFA
Sbjct: 63  MVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFA 122

Query: 125 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 184
           PYG RWRLLRKIS+VHLFS+KAL+D + +R+EE+G L + L R G  P  LG L+ +C  
Sbjct: 123 PYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVV 182

Query: 185 NVLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASE 244
           N LGR MI RR+F  G+  D +A+ F++MV ++M L G FN GDF PSL+WLDL GVA +
Sbjct: 183 NALGREMIGRRLF--GADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGK 242

Query: 245 MKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSK-DEAEDENEKLNDSEIK 304
           MK++HK FD FL+ I++EH       G      D+LSTL+S K  + + +   L D+EIK
Sbjct: 243 MKRLHKRFDAFLSSILKEHEM----NGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIK 302

Query: 305 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 364
           ALLLNMFTAGTDTSASTV+WA+ ELIRHP IM +AQ+ELD VVGRDR V++ D++QLPYL
Sbjct: 303 ALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYL 362

Query: 365 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 424
           QAV+KE FRLHPPTPLS+P +A+ESCEINGYHIPKGSTLL +IWAI+RDP +W+DPL F+
Sbjct: 363 QAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFK 422

Query: 425 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWALPNG 484
           P RFLPGGEK+ VDVKG+DFE+IPFGAGRRIC G+S+GL  +Q LTATL   FDW L  G
Sbjct: 423 PERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGG 482

Query: 485 LLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGYFPAG 518
           + P+KLNM+E+YGLTL+RA PLVVHP+PRL P  +  G
Sbjct: 483 VTPEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVYGLG 511

[Close]
BLAST of Lsi06G014210 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 338.6 bits (867), Expect = 9.2e-93
Identity = 182/503 (36.18%), Postives = 285/503 (56.66%), Query Frame = 0 

Query: 9   AAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGEYGPL 68
           A +++T  ++IL +  F    +  LPPGP+  PIVGN+P +    LH+    +A  +GP+
Sbjct: 21  AVLILTATFSILWY-IFKRSPQPPLPPGPRGLPIVGNLPFL-DPDLHTYFTKLAQSHGPI 80

Query: 69  MYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAPYGPRWRL 128
             L LGS   VV  S S+A ++LK    NF +      A+ + Y   D+V+ PYG  WR+
Sbjct: 81  FKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRM 140

Query: 129 LRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSG--EAPATLGSLLTVCTANVLGRT 188
           LRK+    LFS K LD    +R +EI    + L + G  ++P  +G  L +   N++   
Sbjct: 141 LRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMNLMMNM 200

Query: 189 MISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASEMKKVHK 248
           +    V  +    +     FK ++ ++  L G  N  DFFP L   DL G+  +M    +
Sbjct: 201 LWGGSVKAEDM--ESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVKKMHLYAR 260

Query: 249 WFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIKALLLNMF 308
             D  L + +E+ + L +  GD G  +D L  L+  +D+  D +  +  + +KA+L++M 
Sbjct: 261 DLDAILDRAIEQMQRLRSRDGDDGECKDFLQHLMKLRDQEADSDVPITMNHVKAVLMDMV 320

Query: 309 TAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYLQAVVKET 368
             GT++S +T+E+ + ELI +P++M  AQ+ELD VVG+D  V +  ++ LPY+ AV+KET
Sbjct: 321 VGGTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITSLPYILAVLKET 380

Query: 369 FRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFRPNRFLPG 428
            RL+P  PL VP   +E+  + GY IPK + + +++W+I RDP  W  P EFRP RFL  
Sbjct: 381 LRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPERFL-- 440

Query: 429 GEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWALPNGLLPDKLN 488
            +K   D  G D+  +PFG+GRRIC G+++   M+    ATL HSFDW +P+G +   L+
Sbjct: 441 -DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWTIPDGHV---LD 500

Query: 489 MDEAYGLTLRRAQPLVVHPRPRL 510
           ++E +G+ L+   PLV  P PRL
Sbjct: 501 LEEKFGIVLKLKTPLVALPIPRL 513

[Close]
BLAST of Lsi06G014210 vs. TAIR 10
Match: AT1G01280.1 (cytochrome P450, family 703, subfamily A, polypeptide 2 )

HSP 1 Score: 337.4 bits (864), Expect = 2.0e-92
Identity = 191/512 (37.30%), Postives = 283/512 (55.27%), Query Frame = 0 

Query: 6   LIVAAVVVTLAYAILLHRAFFLRRRFC----LPPGPKPWPIVGNMPQIGGAPLHSLLAAM 65
           L++A++   L   +LL R  +L+   C    LPPGP   PI+GN+ Q+G  P H  LA++
Sbjct: 3   LVLASLFAVLILNVLLWR--WLKASACKAQRLPPGPPRLPILGNLLQLGPLP-HRDLASL 62

Query: 66  AGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAP 125
             +YGPL+YLRLG+VD +         ++L      F SRP    A ++AY   D+  AP
Sbjct: 63  CDKYGPLVYLRLGNVDAITTNDPDTIREILLRQDDVFSSRPKTLAAVHLAYGCGDVALAP 122

Query: 126 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEA--PATLGSLLTVCT 185
            GP W+ +R+I   HL ++K L+     R EE   L++ + +  E   P  L  +L   +
Sbjct: 123 MGPHWKRMRRICMEHLLTTKRLESFTTQRAEEARYLIRDVFKRSETGKPINLKEVLGAFS 182

Query: 186 ANVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVA 245
            N + R ++ ++ FG GS   P EA  F  +  ++  L G    GD+ P   W+D  G  
Sbjct: 183 MNNVTRMLLGKQFFGPGSLVSPKEAQEFLHITHKLFWLLGVIYLGDYLPFWRWVDPSGCE 242

Query: 246 SEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEI 305
            EM+ V K  D+F TKI++EHR       D+ GD D +  LLS     E+    + D EI
Sbjct: 243 KEMRDVEKRVDEFHTKIIDEHRRAKLEDEDKNGDMDFVDVLLSL--PGENGKAHMEDVEI 302

Query: 306 KALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPY 365
           KAL+ +M  A TDTSA T EWA+ E I+ P++M + Q+ELD VVG +R V + DL  L Y
Sbjct: 303 KALIQDMIAAATDTSAVTNEWAMAEAIKQPRVMRKIQEELDNVVGSNRMVDESDLVHLNY 362

Query: 366 LQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEF 425
           L+ VV+ETFR+HP  P  +P  +  +  INGY+IP  + + ++   + R+ K W D  +F
Sbjct: 363 LRCVVRETFRMHPAGPFLIPHESVRATTINGYYIPAKTRVFINTHGLGRNTKIWDDVEDF 422

Query: 426 RPNRFLPGGEKAHVDVK-GNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWALP 485
           RP R  P      V++  G DF+++PF AG+R C G  +G+ MV M  A L H F+W+ P
Sbjct: 423 RPERHWPVEGSGRVEISHGPDFKILPFSAGKRKCPGAPLGVTMVLMALARLFHCFEWSSP 482

Query: 486 NGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRL 510
                  ++  E YG+T+ +A+PL    +PRL
Sbjct: 483 G-----NIDTVEVYGMTMPKAKPLRAIAKPRL 504

[Close]
BLAST of Lsi06G014210 vs. TAIR 10
Match: AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6 )

HSP 1 Score: 335.1 bits (858), Expect = 1.0e-91
Identity = 181/508 (35.63%), Postives = 284/508 (55.91%), Query Frame = 0 

Query: 4   ISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAG 63
           I+L   A+++ +A   +L   F    +  LPPGP+  PIVGN+P +    LH+    +A 
Sbjct: 13  INLTPYAILILIAIFSILWYLFKRSPQPHLPPGPRGLPIVGNLPFL-DPDLHTYFTKLAE 72

Query: 64  EYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAPYG 123
            YGP+  L LGS   VV  + S+A ++LK    NF +      A+ + Y   D+V+ PYG
Sbjct: 73  SYGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYG 132

Query: 124 PRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSG--EAPATLGSLLTVCTAN 183
             WR+LRK+  + L S + L+    +R +EI    + L + G  E+P  +G  + +   N
Sbjct: 133 AEWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLTMMN 192

Query: 184 VLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASEM 243
           +    +    V  +    +     FK ++ ++  L G+ N  DFFP L   DL G+  +M
Sbjct: 193 LTMNMLWGGSVKAEEM--ESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGLVKKM 252

Query: 244 KKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIKAL 303
               +  D  L + +E+ + L    GD G  +D L  L+  KD+  D    +  + +KA+
Sbjct: 253 HVCARELDAILDRAIEQMQLLRTRDGDDGECKDFLQHLMKLKDQEADSEVPITVNHVKAV 312

Query: 304 LLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYLQA 363
           L+++   GTDTS +T+E+A+ ELIR P++M  AQ+ELD VVG+D  + +  +++LP++ A
Sbjct: 313 LVDLVVGGTDTSTNTIEFAMAELIRKPELMKRAQQELDEVVGKDNIIEESHITRLPFISA 372

Query: 364 VVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFRPN 423
           ++KET RL+P  PL VP   +E+  + GY IPK + + +++W+I RDP  W  P EFRP 
Sbjct: 373 IMKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPE 432

Query: 424 RFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWALPNGLL 483
           RFL   +K   D  G D+  +PFG+GRRIC G+++   M+    ATL HSFDW +P G +
Sbjct: 433 RFL---DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWKIPEGHI 492

Query: 484 PDKLNMDEAYGLTLRRAQPLVVHPRPRL 510
              L++ E +G+ L+   PLV  P PRL
Sbjct: 493 ---LDLKEKFGIVLKLKSPLVALPVPRL 511

[Close]
BLAST of Lsi06G014210 vs. TAIR 10
Match: AT3G48280.1 (cytochrome P450, family 71, subfamily A, polypeptide 25 )

HSP 1 Score: 334.7 bits (857), Expect = 1.3e-91
Identity = 179/506 (35.38%), Postives = 290/506 (57.31%), Query Frame = 0 

Query: 11  VVVTLAYAILLHRAFFLRRRFC-----LPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGEY 70
           +++ L ++I+     FL+++        PP P   P++GN+ Q+ G   H  L  ++  Y
Sbjct: 3   MMIILLWSIIFMTILFLKKQLSGKKGKTPPSPPGLPLIGNLHQL-GRHTHRSLCDLSRRY 62

Query: 71  GPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAPYGPR 130
           GPLM L LG V V++ +SA +A+++LK H   F +RP +  ++ + YNNRD+  APYG  
Sbjct: 63  GPLMLLHLGRVPVLIVSSADMAQEILKTHDQAFANRPRSKLSQKLLYNNRDVASAPYGEY 122

Query: 131 WRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTANVLGR 190
           WR ++ +  +HL S+K +   R +REEEI +++  + +S   P  +  +L   T +V+ R
Sbjct: 123 WRQMKSVCVIHLLSNKMVRSFRDVREEEITLMMAKIRKSSSLPFNVSKVLECLTNDVICR 182

Query: 191 TMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLD-LLGVASEMKKV 250
             + R+      GG+ +   FK + +++  L G F+ G F P L W+D + G  +++ K+
Sbjct: 183 VALGRKY-----GGETD---FKKLTDRLSELLGTFSIGSFVPWLAWVDWIRGWDAQLDKM 242

Query: 251 HKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIKALLLN 310
            K  DDF  K+V++H +     GDR    DL+  LL  K E +    ++    IKA+ L+
Sbjct: 243 GKDLDDFFEKVVQDHED-----GDRRDGTDLIDALLRVKRE-KSPGFEIERVSIKAITLD 302

Query: 311 MFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYLQAVVK 370
           +F  G+DTS + +EWA+TEL+RHPK +   Q+E+ T+      VS+ D+  + YL+AV+K
Sbjct: 303 VFVGGSDTSFTLLEWAMTELLRHPKSLNRLQEEVRTICKGKSRVSEDDIQGMKYLKAVIK 362

Query: 371 ETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWA-DPLEFRPNRF 430
           E  RLHPP P+  P  + E  ++  YHIP G+ ++++ WAI R+   W  D  EF+P R 
Sbjct: 363 EALRLHPPFPMMAPHESTEDVKLRDYHIPAGTQVMMNAWAIGREVATWGPDAEEFKPERH 422

Query: 431 LPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLMMVQMLTATLAHSFDWALPNGLLPD 490
           L       VD +G +FE++PFGAGRRIC  +S  +++ +++ A L H FDW LP     D
Sbjct: 423 L----DTSVDFRGQNFELLPFGAGRRICPAVSFAVVLNEVVLANLVHGFDWKLPEESKED 482

Query: 491 KLNMDEAYGLTLRRAQPLVVHPRPRL 510
           K ++ E+ G ++ R  PL     P L
Sbjct: 483 KTDVAESSGFSVHREFPLYAVASPYL 489

The following BLAST results are available for this feature:
BLAST of Lsi06G014210 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SBQ98.7e-18963.80Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1[more]
Q9SD857.9e-18261.00Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1[more]
A0A4D6Q4158.8e-16556.95Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN... [more]
Q7G6021.5e-16157.20Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Q8LM923.9e-15754.21Flavonoid 3'-monooxygenase CYP75B4 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
BLAST of Lsi06G014210 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7UTD54.3e-25585.91Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3BVU84.3e-25585.91flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1[more]
A0A6J1EZJ72.3e-24582.63flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV... [more]
A0A6J1L0D67.5e-24482.05flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1[more]
A0A0A0LP991.9e-22679.33Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1[more]
BLAST of Lsi06G014210 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038878698.11.8e-26089.32flavonoid 3'-monooxygenase [Benincasa hispida][more]
XP_008453263.18.8e-25585.91PREDICTED: flavonoid 3'-monooxygenase [Cucumis melo] >KAA0057987.1 flavonoid 3'-... [more]
XP_004138192.14.1e-25285.05flavonoid 3'-monooxygenase [Cucumis sativus] >KGN63725.2 hypothetical protein Cs... [more]
XP_023529494.12.6e-24682.50flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo] >XP_023529495.1 flavonoi... [more]
XP_022933611.14.8e-24582.63flavonoid 3'-monooxygenase [Cucurbita moschata][more]
BLAST of Lsi06G014210 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G07990.15.6e-18361.00Cytochrome P450 superfamily protein [more]
AT4G12310.19.2e-9336.18cytochrome P450, family 706, subfamily A, polypeptide 5 [more]
AT1G01280.12.0e-9237.30cytochrome P450, family 703, subfamily A, polypeptide 2 [more]
AT4G12320.11.0e-9135.63cytochrome P450, family 706, subfamily A, polypeptide 6 [more]
AT3G48280.11.3e-9135.38cytochrome P450, family 71, subfamily A, polypeptide 25 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 276..299
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 7..508
NoneNo IPR availablePANTHERPTHR47956:SF3FLAVONOID 3'-MONOOXYGENASE CYP75B4coord: 7..508
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 86..107
score: 23.48
coord: 62..81
score: 35.95
coord: 360..378
score: 39.36
coord: 297..314
score: 32.51
coord: 441..451
score: 57.81
coord: 317..343
score: 35.71
coord: 401..425
score: 38.36
coord: 451..474
score: 23.36
IPR001128Cytochrome P450PRINTSPR00385P450coord: 308..325
score: 38.24
coord: 451..462
score: 26.24
coord: 442..451
score: 60.9
coord: 361..372
score: 45.39
IPR001128Cytochrome P450PFAMPF00067p450coord: 34..486
e-value: 1.6E-107
score: 360.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 28..509
e-value: 5.1E-130
score: 436.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 34..508
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 444..453

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G014210.1Lsi06G014210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen