Lsi06G013690 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G013690
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPhosphopyruvate hydratase
Locationchr06: 24266524 .. 24270211 (+)
RNA-Seq ExpressionLsi06G013690
SyntenyLsi06G013690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACAAATGTCCATTGTCCAGTGAGAGTCGCTTTCAGTGTTCTCGTCGCCAATTCCCAACAACCACGCTGCTGGCTGCTGCTCTGCTTTGAACGCCCCTTTTTGGCGGACTTTCCCACTCTATTCTTCTTCTTCTTCTTCTTCTATATATATATATTTATATATATTCATTCCCCATTTCCATTTCTCTGCATCAAAACTTCCATGGCTTCTCTTCCTTCCCCACCCACCATGAATCTCCTCGAAAAATCCTTCCTTTCTTCAACATCTCAATCTTTCATTACCTCAATACCCTCTTCTTCTTCTTCTTCTTCGAAACCCCTTGGCTCCCATGCTATTCGATGTTCTGTTGCTGTGGCGCCTTCTGCTTCCACCAAGGTCTCGAAGGAATTCAAGTTGAAGTCCGTGAAGGCTCGGCAGATCATTGACAGTAGAGGCAATCCCACGGTCGAGGTTGATTTGATTACTGACGATCTCTACCGATCCGCTGTCCCCAGCGGGGCTTCCACTGGGATCTATGAGGCTTTGGAGCTTAGAGATGGGGATAAAACTGTTTATGGAGGTAAAGGTGTGCTTACTGCTGTTAAGAACATCAATGATATATTGGCTCCTAAGCTCGTTGGCGTCGATGTCAGGTCGATTCAATTCTTTCTTTTAATTTGTTTTAATAGACATCAATTTCAACGTTATCGTGCTAATTGCCATTGTGTTCATCTGATTAAATTGTTTCTTCTACTTTCGGACATAATATGGACAAGACTCTTCTCTCCGAGTGTTTTATTGTTTCGGGTTTGCAAAACTTGAACTAGTTTTCTGGTCAAACGAGCTTTTTGTCCTTTTATTTCGTTTGATAGTTGACTGCAAATCTGCTTTGTCATTTAAGTAGACCTTCTATAGTATATGTCCATGAGTCTAGACCACAAATTGCATAATGAGATTTGTTTTTGAAACTTGTGGCCTGTTTAAAACCTTAAAAACACTTTTTTTTTTCACTTGAAATTACTTTTTCAAATATTAGGAAAATCATTTCAAACAGGCCTTCAATTACTTGCATCGTCCTTTTCATTCTGATTGTGAGGCTTGTGTAAAAGGTGGCTTAGTTCACACCGCGTTTTCATCCTGATTGTTGCTAGACAAGGACGTGAATGGAACAGAATAGATAGTGGAAAATTTCGTAGTTGGGGATTCTCAAACTATATGGTTTTGTGTTGAACTTACTAATATCTGACTGGGAAAATTGGTAGTCATTTTATTCAATTTATTACTGAAGATGTAAAATTTCAAGCTTTTTCATTGGTTCGTCTTTTTGTATGTGTTTTCTTTATATATATATATAAGGAATTGAACCTTCATTGAAGCATTCAAAATAACAGGCCCACGTAAAGGAAGTCTCTCTAAAGAAAGGGTATGTCATTGGTCTGTCTTGTTTGAGAAGAGAATCAAATTTATTGACAATGAAATAAGTAGTAAAACTCCCAAACACTTAGGGTGTGTGTTACAAGAAGAATGACTATAATCAGAAAAAAACAAAAGGGTGACTTTTATGGGAGTTGTTTCACTTTTCAATACTTCTGCTTCCTAACCTAAGTTTGTTAACTTCTGTAGGAATCAAGAAGAAGTTGATGCTATCATGCTGGAAATTGATGGAACCCCAAACAAGTCGAAACTTGGGGCAAATGCTATACTAGGAGTTTCTTTGAGCGTCTGCAGAGCTGGTGCTGGCGCCAAGGGACTGCCATTGTATCGGCACATCCAAGAATTATCAGGCACCAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTCATAAATGGGGGTAGCCATGCTGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCTGTAGGAGCTACATCTTTCGCAGAGGCACTTCGCATGGGAAGTGAAGGTTAGCAGCTTCTCCAGTGCTGAAATTTTCTTTTCCATTGTAATGGTGTTAGTTTATGACTCATTTTGCACCTCATAAATATGCTTGTTTCTGTCATTCATATATACAGTCGTCATCATTTTCCTACTTAACCAGTCATTTTTTAGTAGTCTGACCTTAGTATTTTTTTATGAACAGTTTATCACACACTGAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTCGCTCCAAATGTTCAAGATAACAGAGAAGGACTGGTGTTGCTTATTGATGCCATTGAAAAGGCTGGTTATACAGGAAAGGTACAGGATTCTAAATTTCCCTTTTTGTTTTGGAATGGTTAGCAGTTTTCTTACATGTCCTGCTAAGACAAAAAAATAAAAATTTACTTTGATACAGATCAAAATAGGAATGGATGTTGCAGCTTCAGAGTTTTTCACTAAAGAGGGAAAATATGATCTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTTCACTCAGCTCATGGCCTTGGCGAACTCTATAAACAGTTTGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGATCAAGATGATTGGAGCTCCTGGGCTTCTCTACAATCTTCTGTTGATATCCAACTTGTGGGAGATGACTTGCTGGTAACAAATCCAAAGAGAATTGCCGAAGCCATTCAGAAAAAAGCTTGCAATGCTTTACTTCTGAAGGTAAGCAGCCTCTTATTTCGTTCTTTCCTTTCTCTCTATATTTCAAACTAATTGTAGGATTTAGGTTAGTCCTTAAGAACTTGTTTCCTGAATCTTTGGTTCTCCATTGATTTAGGTTCACCTGTCTCCATATGCAGTTAGAAACATGTGATTTTGATGCTGCAATTTGCTCATATGTTAGAAATTTCTCCTGCAGGTTAACCAGATTGGATCAGTGACTGAATCAATTCAAGCTGCTCTTGATTCCAAGGCTGCTGGCTGGGGAGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCTGATCTTTCTGTTGGCTTAGCTAGTGGACAGGTTTCACTCATCTTCAGCTCCTGTCCTATAACTACAAGTCTATAACACATGTATGTTGTTGACAATACATTCACAACTGAATATTGTGTTACTATTCTACAGATTAAAACTGGAGCTCCATGCCGAAGTGAACGGTTAGCGAAATATAATCAGGTAAAGTTTGATCAAATCAGAACTTCCAAAGTTATTGTCCTTGTATTGCTACAACAATTCTAGTTTTACTCAATATATCTACGTATATTTCTTTTTTTGGATGCAGCTTCTTCGAATTGAAGAAGAACTTGGCAGTGTTCGATATGCTGGTGCGTCTTTCAGATCACCTTAGGAGATAAAAGTTCAAGGTGACAGTGTTAATGTATCAATTTTAGGCTGAAGGATGGCTTGATAGAAGAAAATGTAGAGGATTTCTGCAGAAATAAGATGCAAAACTTTTCTGTCAGAGGTGTTCGAATCTGACACGTTACATAGCTGATTCGAGTGTTACCATGAGAGTATTTAAATTTTTGTTTTGAACTATTAGTTTCAAAATAATGCAGTGCTTTGATTCCCATACTACCATATGGGATTCTCTTAGTTTGAAAGCAATTCCATACAGCCAAATATCAGAAACCTGATTAGTTCATCTAAATTATGTCATCAAACATTGAGCAGATGAAAGATATTGCTAAGACTTAGCCATTAACTCGA

mRNA sequence

AACAAATGTCCATTGTCCAGTGAGAGTCGCTTTCAGTGTTCTCGTCGCCAATTCCCAACAACCACGCTGCTGGCTGCTGCTCTGCTTTGAACGCCCCTTTTTGGCGGACTTTCCCACTCTATTCTTCTTCTTCTTCTTCTTCTATATATATATATTTATATATATTCATTCCCCATTTCCATTTCTCTGCATCAAAACTTCCATGGCTTCTCTTCCTTCCCCACCCACCATGAATCTCCTCGAAAAATCCTTCCTTTCTTCAACATCTCAATCTTTCATTACCTCAATACCCTCTTCTTCTTCTTCTTCTTCGAAACCCCTTGGCTCCCATGCTATTCGATGTTCTGTTGCTGTGGCGCCTTCTGCTTCCACCAAGGTCTCGAAGGAATTCAAGTTGAAGTCCGTGAAGGCTCGGCAGATCATTGACAGTAGAGGCAATCCCACGGTCGAGGTTGATTTGATTACTGACGATCTCTACCGATCCGCTGTCCCCAGCGGGGCTTCCACTGGGATCTATGAGGCTTTGGAGCTTAGAGATGGGGATAAAACTGTTTATGGAGGTAAAGGTGTGCTTACTGCTGTTAAGAACATCAATGATATATTGGCTCCTAAGCTCGTTGGCGTCGATGTCAGGAATCAAGAAGAAGTTGATGCTATCATGCTGGAAATTGATGGAACCCCAAACAAGTCGAAACTTGGGGCAAATGCTATACTAGGAGTTTCTTTGAGCGTCTGCAGAGCTGGTGCTGGCGCCAAGGGACTGCCATTGTATCGGCACATCCAAGAATTATCAGGCACCAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTCATAAATGGGGGTAGCCATGCTGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCTGTAGGAGCTACATCTTTCGCAGAGGCACTTCGCATGGGAAGTGAAGTTTATCACACACTGAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTCGCTCCAAATGTTCAAGATAACAGAGAAGGACTGGTGTTGCTTATTGATGCCATTGAAAAGGCTGGTTATACAGGAAAGATCAAAATAGGAATGGATGTTGCAGCTTCAGAGTTTTTCACTAAAGAGGGAAAATATGATCTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTTCACTCAGCTCATGGCCTTGGCGAACTCTATAAACAGTTTGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGATCAAGATGATTGGAGCTCCTGGGCTTCTCTACAATCTTCTGTTGATATCCAACTTGTGGGAGATGACTTGCTGGTAACAAATCCAAAGAGAATTGCCGAAGCCATTCAGAAAAAAGCTTGCAATGCTTTACTTCTGAAGGTTAACCAGATTGGATCAGTGACTGAATCAATTCAAGCTGCTCTTGATTCCAAGGCTGCTGGCTGGGGAGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCTGATCTTTCTGTTGGCTTAGCTAGTGGACAGATTAAAACTGGAGCTCCATGCCGAAGTGAACGGTTAGCGAAATATAATCAGCTTCTTCGAATTGAAGAAGAACTTGGCAGTGTTCGATATGCTGGTGCGTCTTTCAGATCACCTTAGGAGATAAAAGTTCAAGGTGACAGTGTTAATGTATCAATTTTAGGCTGAAGGATGGCTTGATAGAAGAAAATGTAGAGGATTTCTGCAGAAATAAGATGCAAAACTTTTCTGTCAGAGGTGTTCGAATCTGACACGTTACATAGCTGATTCGAGTGTTACCATGAGAGTATTTAAATTTTTGTTTTGAACTATTAGTTTCAAAATAATGCAGTGCTTTGATTCCCATACTACCATATGGGATTCTCTTAGTTTGAAAGCAATTCCATACAGCCAAATATCAGAAACCTGATTAGTTCATCTAAATTATGTCATCAAACATTGAGCAGATGAAAGATATTGCTAAGACTTAGCCATTAACTCGA

Coding sequence (CDS)

ATGGCTTCTCTTCCTTCCCCACCCACCATGAATCTCCTCGAAAAATCCTTCCTTTCTTCAACATCTCAATCTTTCATTACCTCAATACCCTCTTCTTCTTCTTCTTCTTCGAAACCCCTTGGCTCCCATGCTATTCGATGTTCTGTTGCTGTGGCGCCTTCTGCTTCCACCAAGGTCTCGAAGGAATTCAAGTTGAAGTCCGTGAAGGCTCGGCAGATCATTGACAGTAGAGGCAATCCCACGGTCGAGGTTGATTTGATTACTGACGATCTCTACCGATCCGCTGTCCCCAGCGGGGCTTCCACTGGGATCTATGAGGCTTTGGAGCTTAGAGATGGGGATAAAACTGTTTATGGAGGTAAAGGTGTGCTTACTGCTGTTAAGAACATCAATGATATATTGGCTCCTAAGCTCGTTGGCGTCGATGTCAGGAATCAAGAAGAAGTTGATGCTATCATGCTGGAAATTGATGGAACCCCAAACAAGTCGAAACTTGGGGCAAATGCTATACTAGGAGTTTCTTTGAGCGTCTGCAGAGCTGGTGCTGGCGCCAAGGGACTGCCATTGTATCGGCACATCCAAGAATTATCAGGCACCAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTCATAAATGGGGGTAGCCATGCTGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCTGTAGGAGCTACATCTTTCGCAGAGGCACTTCGCATGGGAAGTGAAGTTTATCACACACTGAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTCGCTCCAAATGTTCAAGATAACAGAGAAGGACTGGTGTTGCTTATTGATGCCATTGAAAAGGCTGGTTATACAGGAAAGATCAAAATAGGAATGGATGTTGCAGCTTCAGAGTTTTTCACTAAAGAGGGAAAATATGATCTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTTCACTCAGCTCATGGCCTTGGCGAACTCTATAAACAGTTTGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGATCAAGATGATTGGAGCTCCTGGGCTTCTCTACAATCTTCTGTTGATATCCAACTTGTGGGAGATGACTTGCTGGTAACAAATCCAAAGAGAATTGCCGAAGCCATTCAGAAAAAAGCTTGCAATGCTTTACTTCTGAAGGTTAACCAGATTGGATCAGTGACTGAATCAATTCAAGCTGCTCTTGATTCCAAGGCTGCTGGCTGGGGAGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCTGATCTTTCTGTTGGCTTAGCTAGTGGACAGATTAAAACTGGAGCTCCATGCCGAAGTGAACGGTTAGCGAAATATAATCAGCTTCTTCGAATTGAAGAAGAACTTGGCAGTGTTCGATATGCTGGTGCGTCTTTCAGATCACCTTAG

Protein sequence

MASLPSPPTMNLLEKSFLSSTSQSFITSIPSSSSSSSKPLGSHAIRCSVAVAPSASTKVSKEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP
Homology
BLAST of Lsi06G013690 vs. ExPASy Swiss-Prot
Match: Q9C9C4 (Enolase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ENO1 PE=1 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 3.4e-227
Identity = 401/482 (83.20%), Postives = 439/482 (91.08%), Query Frame = 0

Query: 13  LEKSFLSS---TSQSFITSIPSSSSSSSKPLGSHAIRCSVAVAPSASTKVSKEFKLKSVK 72
           L++SFLS    + + ++ S PS             ++CSV         V+KE ++K VK
Sbjct: 10  LQRSFLSPSRVSGERYLESAPSCLR-----FRRSGVQCSV---------VAKECRVKGVK 69

Query: 73  ARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYGGKGVLTAVKN 132
           ARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK+VYGGKGVL A+KN
Sbjct: 70  ARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLQAIKN 129

Query: 133 INDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPL 192
           IN+++APKL+GVDVRNQ +VDA+MLE+DGTPNKSKLGANAILGVSLSVCRAGAGAKG+PL
Sbjct: 130 INELVAPKLIGVDVRNQADVDALMLELDGTPNKSKLGANAILGVSLSVCRAGAGAKGVPL 189

Query: 193 YRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEALRMGSEVYH 252
           Y+HIQE SGTKELVMPVPAFNVINGGSHAGN+LAMQEFMILPVGATSF+EA +MGSEVYH
Sbjct: 190 YKHIQETSGTKELVMPVPAFNVINGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSEVYH 249

Query: 253 TLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASE 312
           TLKGIIK KYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASE
Sbjct: 250 TLKGIIKTKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASE 309

Query: 313 FFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQ 372
           FF K+G+YDLNFKKQPNDGAHV SA  L +LY++F+KDFPIVSIEDPFDQDDWSSWASLQ
Sbjct: 310 FFMKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFDQDDWSSWASLQ 369

Query: 373 SSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVM 432
           SSVDIQLVGDDLLVTNPKRIAEAI+K++CNALLLKVNQIG+VTESIQAALDSKAAGWGVM
Sbjct: 370 SSVDIQLVGDDLLVTNPKRIAEAIKKQSCNALLLKVNQIGTVTESIQAALDSKAAGWGVM 429

Query: 433 VSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFR 492
           VSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERL+KYNQLLRIEEELG+VRYAG +FR
Sbjct: 430 VSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLSKYNQLLRIEEELGNVRYAGEAFR 477

BLAST of Lsi06G013690 vs. ExPASy Swiss-Prot
Match: P15007 (Enolase OS=Drosophila melanogaster OX=7227 GN=Eno PE=1 SV=2)

HSP 1 Score: 600.5 bits (1547), Expect = 1.7e-170
Identity = 301/440 (68.41%), Postives = 362/440 (82.27%), Query Frame = 0

Query: 56  STKVSKEFKLKSVKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGD 115
           ST  S E  +K++KARQI DSRGNPTVEVDL T+  L+R+AVPSGASTG++EALELRD D
Sbjct: 61  STCDSNEMTIKAIKARQIYDSRGNPTVEVDLTTELGLFRAAVPSGASTGVHEALELRDND 120

Query: 116 KTVYGGKGVLTAVKNINDILAPKLV--GVDVRNQEEVDAIMLEIDGTPNKSKLGANAILG 175
           K  Y GK VL AV ++ND L P+L+   +DV +Q  +D  M+++DGT NKSK GANAILG
Sbjct: 121 KANYHGKSVLKAVGHVNDTLGPELIKANLDVVDQASIDNFMIKLDGTENKSKFGANAILG 180

Query: 176 VSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPV 235
           VSL+V +AGA  KG+PLY+HI +L+G KE+++PVPAFNVINGGSHAGN LAMQEFMILP 
Sbjct: 181 VSLAVAKAGAAKKGVPLYKHIADLAGNKEIILPVPAFNVINGGSHAGNKLAMQEFMILPT 240

Query: 236 GATSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEK 295
           GATSF EA++MGSEVYH LK +IKAK+G DA  VGDEGGFAPN+Q N+E L L+ DAI K
Sbjct: 241 GATSFTEAMKMGSEVYHHLKNVIKAKFGLDATAVGDEGGFAPNIQSNKEALNLISDAIAK 300

Query: 296 AGYTGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVS 355
           AGYTGKI+IGMDVAASEF+ K+G+YDL+FK + +D +    A  L  LY++F+KDFPIVS
Sbjct: 301 AGYTGKIEIGMDVAASEFY-KDGQYDLDFKNEKSDKSQWLPADKLANLYQEFIKDFPIVS 360

Query: 356 IEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVT 415
           IEDPFDQD W +W++L    DIQ+VGDDL VTNPKRIA A++KKACN LLLKVNQIG+VT
Sbjct: 361 IEDPFDQDHWEAWSNLTGCTDIQIVGDDLTVTNPKRIATAVEKKACNCLLLKVNQIGTVT 420

Query: 416 ESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLL 475
           ESI A L +K  GWG MVSHRSGETED+FI DL VGL++GQIKTGAPCRSERLAKYNQ+L
Sbjct: 421 ESIAAHLLAKKNGWGTMVSHRSGETEDSFIGDLVVGLSTGQIKTGAPCRSERLAKYNQIL 480

Query: 476 RIEEELGS-VRYAGASFRSP 492
           RIEEE+G+ V++AG SFR P
Sbjct: 481 RIEEEIGAGVKFAGKSFRKP 499

BLAST of Lsi06G013690 vs. ExPASy Swiss-Prot
Match: Q54RK5 (Enolase A OS=Dictyostelium discoideum OX=44689 GN=enoA PE=3 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 3.8e-170
Identity = 302/431 (70.07%), Postives = 358/431 (83.06%), Query Frame = 0

Query: 65  LKSVKARQIIDSRGNPTVEVDLITD-----DLYRSAVPSGASTGIYEALELRDGDKTVYG 124
           +KS+KAR+I++SRGNPTVEVDL T+       +R+AVPSGASTGIYEA+ELRDGDK+ Y 
Sbjct: 4   IKSIKAREILNSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYEAVELRDGDKSRYL 63

Query: 125 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCR 184
           GKGVL A+KNI +++ P ++G  V +Q  +D +M+++DGTPNK KLGANAIL VSL+VCR
Sbjct: 64  GKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPNKGKLGANAILAVSLAVCR 123

Query: 185 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAE 244
           AGA  + LPLY++I E++GTK + +PVPAFNVINGGSHAGN LAMQEFMILPVGA  F E
Sbjct: 124 AGAADRNLPLYKYISEIAGTK-MRLPVPAFNVINGGSHAGNKLAMQEFMILPVGAKDFNE 183

Query: 245 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 304
           A RMGSEVYH LK +I  +YGQDA NVGDEGGFAP +Q N+EGL LL  AIEKAGYTG +
Sbjct: 184 AYRMGSEVYHNLKNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLV 243

Query: 305 KIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 364
           KIGMD AASEF  + G YDL+FK + NDG+ V S   LG+LY++F+K++PI+SIEDPFDQ
Sbjct: 244 KIGMDCAASEFKVENG-YDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQ 303

Query: 365 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAAL 424
           DDW S+  L +SVDIQ+VGDDLLVTNP+RI   I+KKACNALLLKVNQIGSVTESI+AAL
Sbjct: 304 DDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAAL 363

Query: 425 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 484
           DSK A WGVMVSHRSGETED FIADL VGL +GQIKTGAPCRSERLAKYNQL+RI EELG
Sbjct: 364 DSKNASWGVMVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELG 423

Query: 485 -SVRYAGASFR 490
            +  YAG +FR
Sbjct: 424 ENHNYAGLTFR 432

BLAST of Lsi06G013690 vs. ExPASy Swiss-Prot
Match: P42894 (Enolase OS=Neocallimastix frontalis OX=4757 PE=3 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 4.2e-169
Identity = 305/434 (70.28%), Postives = 359/434 (82.72%), Query Frame = 0

Query: 65  LKSVKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKTVYGGKGV 124
           +  V ARQI DSRGNPTVEV++ TD  L+R+AVPSGASTG++EALELRDG K  Y GKGV
Sbjct: 3   ITKVHARQIFDSRGNPTVEVEVTTDKGLFRAAVPSGASTGVHEALELRDGIKADYVGKGV 62

Query: 125 LTAVKNINDILAPKLV--GVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCRAG 184
           L AV+N+N  +AP LV   +DV+NQ+ VD  +L++DGTPNKSKLGANAILGVSL+V RAG
Sbjct: 63  LKAVENVNKTIAPALVAANLDVKNQKAVDDFLLKLDGTPNKSKLGANAILGVSLAVARAG 122

Query: 185 AGAKGLPLYRHIQELSGTK-ELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEA 244
           A  KG+PLY+H+ EL+G K   ++PVP+ NV+NGGSHAGN LAMQEFMILP GA SF EA
Sbjct: 123 AADKGVPLYQHLGELAGNKGPWILPVPSMNVLNGGSHAGNKLAMQEFMILPTGAKSFTEA 182

Query: 245 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIK 304
           L+MGSEVYH LK +IKAKYGQDACNVGDEGGFAPN+QDN+EGL LL +AI KAGYTGK+K
Sbjct: 183 LKMGSEVYHALKSVIKAKYGQDACNVGDEGGFAPNIQDNKEGLELLNEAIAKAGYTGKVK 242

Query: 305 IGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD 364
           IGMDVA+SEF+ K+GKYDL+FK   +D +   S   LG+ YK+   ++PIVSIEDP+DQD
Sbjct: 243 IGMDVASSEFY-KDGKYDLDFKNPNSDPSKWISGEELGQFYKEITSEYPIVSIEDPYDQD 302

Query: 365 DWSSWASLQSSVD--IQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAA 424
           D+ SW+  ++ +   IQ+VGDDL VTNPKRIA AI+KKACN LLLKVNQIG+V+ESIQAA
Sbjct: 303 DFESWSKFRADMQDKIQIVGDDLTVTNPKRIAMAIEKKACNGLLLKVNQIGTVSESIQAA 362

Query: 425 LDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEEL 484
           LD+   GWGVMVSHRSGETED FIADL VGL SGQIKTGAPCRSERLAKYNQLLRIEEEL
Sbjct: 363 LDAFNDGWGVMVSHRSGETEDTFIADLVVGLKSGQIKTGAPCRSERLAKYNQLLRIEEEL 422

Query: 485 G-SVRYAGASFRSP 492
           G +  YAG +FR P
Sbjct: 423 GANATYAGENFRRP 435

BLAST of Lsi06G013690 vs. ExPASy Swiss-Prot
Match: P15429 (Beta-enolase OS=Rattus norvegicus OX=10116 GN=Eno3 PE=1 SV=3)

HSP 1 Score: 594.0 bits (1530), Expect = 1.6e-168
Identity = 303/431 (70.30%), Postives = 357/431 (82.83%), Query Frame = 0

Query: 65  LKSVKARQIIDSRGNPTVEVDLIT-DDLYRSAVPSGASTGIYEALELRDGDKTVYGGKGV 124
           ++ + AR+I+DSRGNPTVEVDL T    +R+AVPSGASTGIYEALELRDGDK+ Y GKGV
Sbjct: 3   MQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKSRYLGKGV 62

Query: 125 LTAVKNINDILAPKLV--GVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCRAG 184
           L AV++IN  L P L+   + V +QE+VD  M+E+DGT NKSK GANAILGVSL+VC+AG
Sbjct: 63  LKAVEHINKTLGPALLEKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAG 122

Query: 185 AGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEAL 244
           A  KG+PLYRHI +L+G  +LV+PVPAFNVINGGSHAGN LAMQEFMILPVGA+SF EA+
Sbjct: 123 AAEKGVPLYRHIADLAGNPDLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAM 182

Query: 245 RMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKI 304
           R+G+EVYH LKG+IKAKYG+DA NVGDEGGFAPN+ +N E L LL  AI+ AGY  K+ I
Sbjct: 183 RIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVI 242

Query: 305 GMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDD 364
           GMDVAASEF+ + GKYDL+F K P+D A   S   LGELYK F+K++P+VSIEDPFDQDD
Sbjct: 243 GMDVAASEFY-RNGKYDLDF-KSPDDPARHISGEKLGELYKSFIKNYPVVSIEDPFDQDD 302

Query: 365 WSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALDS 424
           W++W S  S VDIQ+VGDDL VTNPKRIA+A++KKACN LLLKVNQIGSVTESIQA   +
Sbjct: 303 WATWTSFLSGVDIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLA 362

Query: 425 KAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG-S 484
           ++ GWGVMVSHRSGETED FIADL VGL +GQIKTGAPCRSERLAKYNQL+RIEE LG  
Sbjct: 363 QSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDK 422

Query: 485 VRYAGASFRSP 492
             +AG  FR+P
Sbjct: 423 AVFAGRKFRNP 431

BLAST of Lsi06G013690 vs. ExPASy TrEMBL
Match: A0A5A7US84 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003390 PE=3 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 8.2e-261
Identity = 473/496 (95.36%), Postives = 475/496 (95.77%), Query Frame = 0

Query: 1   MASLPSPPTMNLLEKSFLSSTSQSFITSIP-----SSSSSSSKPLGSHAIRCSVAVAPSA 60
           MASLPSPPTMNLL K    S  QSFI SIP     SSSSSSSKPL S  IRCSVAVAPS 
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STKVSKEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           ST VSKEFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180
           TVYGGKGVLTAVKNI DILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNIKDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE IQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 492
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of Lsi06G013690 vs. ExPASy TrEMBL
Match: A0A1S3BX56 (Phosphopyruvate hydratase OS=Cucumis melo OX=3656 GN=LOC103494088 PE=3 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 8.2e-261
Identity = 473/496 (95.36%), Postives = 475/496 (95.77%), Query Frame = 0

Query: 1   MASLPSPPTMNLLEKSFLSSTSQSFITSIP-----SSSSSSSKPLGSHAIRCSVAVAPSA 60
           MASLPSPPTMNLL K    S  QSFI SIP     SSSSSSSKPL S  IRCSVAVAPS 
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STKVSKEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           ST VSKEFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180
           TVYGGKGVLTAVKNI DILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNIKDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE IQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 492
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of Lsi06G013690 vs. ExPASy TrEMBL
Match: A0A5D3DYS6 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00060 PE=3 SV=1)

HSP 1 Score: 908.7 bits (2347), Expect = 1.1e-260
Identity = 473/496 (95.36%), Postives = 475/496 (95.77%), Query Frame = 0

Query: 1   MASLPSPPTMNLLEKSFLSSTSQSFITSIP-----SSSSSSSKPLGSHAIRCSVAVAPSA 60
           MASLPSPPTMNLL K    S  QSFI SIP     SSSSSSSKPL S  IRCSVAVAPS 
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STKVSKEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           ST VSKEFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180
           TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFTK GKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE IQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 492
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of Lsi06G013690 vs. ExPASy TrEMBL
Match: A0A0A0LPK6 (Phosphopyruvate hydratase OS=Cucumis sativus OX=3659 GN=Csa_1G014400 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.4e-260
Identity = 472/492 (95.93%), Postives = 475/492 (96.54%), Query Frame = 0

Query: 1   MASLPSPPTMNLLEKSFLSSTSQSFITSIP-SSSSSSSKPLGSHAIRCSVAVAPSASTKV 60
           MASLPSP TMNLL KS   S  QSFI  IP SSSSSSSKPL S  IRCSVAVAPS ST V
Sbjct: 1   MASLPSPHTMNLLNKSLSLSPPQSFIALIPSSSSSSSSKPLRSPTIRCSVAVAPSVSTNV 60

Query: 61  SKEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 120
           SKEFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG
Sbjct: 61  SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 120

Query: 121 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCR 180
           GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKS+LGANAILGVSLSVCR
Sbjct: 121 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR 180

Query: 181 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAE 240
           AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+SFAE
Sbjct: 181 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 240

Query: 241 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 300
           ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI
Sbjct: 241 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 300

Query: 301 KIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 360
           KIGMDVAASEFFT EGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ
Sbjct: 301 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 360

Query: 361 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAAL 420
           DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE IQKKACNALLLKVNQIGSVTESIQAAL
Sbjct: 361 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL 420

Query: 421 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 480
           DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG
Sbjct: 421 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 480

Query: 481 SVRYAGASFRSP 492
           SVRYAGASFRSP
Sbjct: 481 SVRYAGASFRSP 492

BLAST of Lsi06G013690 vs. ExPASy TrEMBL
Match: A0A6J1BZW1 (Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111007005 PE=3 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 2.3e-255
Identity = 461/496 (92.94%), Postives = 471/496 (94.96%), Query Frame = 0

Query: 1   MASLPSPPTMNLLEKSFLSSTSQSFITSIPSSSSSSSKP-----LGSHAIRCSVAVAPSA 60
           MASL SPP MNLL  + L S S S I SIPSSSSSSS       L S A+RCSVAVAPSA
Sbjct: 1   MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSA 60

Query: 61  STKVSKEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           +TK SKE+KLKSVKARQIIDSRGNPTVEVDL+TDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  ATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180
           +VYGGKGVLTAVKNINDILAPKLVGVDVRNQE+VDAIMLEIDGT NKSKLGANAILGVSL
Sbjct: 121 SVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYH LKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFTK+GKYDLNFKKQPNDGAHVHSAHGLGELYKQF+KDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSW+SLQSSVDIQLVGDDLLVTNPKRIAEAI KKAC ALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACTALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 492
           EELGSVRYAG SFRSP
Sbjct: 481 EELGSVRYAGESFRSP 496

BLAST of Lsi06G013690 vs. NCBI nr
Match: XP_038879680.1 (enolase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 924.1 bits (2387), Expect = 5.1e-265
Identity = 476/491 (96.95%), Postives = 482/491 (98.17%), Query Frame = 0

Query: 1   MASLPSPPTMNLLEKSFLSSTSQSFITSIPSSSSSSSKPLGSHAIRCSVAVAPSASTKVS 60
           MASLPSPPTMNLLEKS L S+ QSFI+SIP SSSSSSKPL SHAIRCSVAVAPS STK S
Sbjct: 1   MASLPSPPTMNLLEKSLLPSSPQSFISSIP-SSSSSSKPLRSHAIRCSVAVAPSVSTKAS 60

Query: 61  KEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYGG 120
           KEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYGG
Sbjct: 61  KEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYGG 120

Query: 121 KGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCRA 180
           KGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCRA
Sbjct: 121 KGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCRA 180

Query: 181 GAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEA 240
           GAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA SFAEA
Sbjct: 181 GAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAISFAEA 240

Query: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIK 300
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDN+EGLVLLIDAIEKAGYTGKIK
Sbjct: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNKEGLVLLIDAIEKAGYTGKIK 300

Query: 301 IGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD 360
           IGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD
Sbjct: 301 IGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD 360

Query: 361 DWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 420
           DWSSWASLQSSVDIQLVGDDLLVTNPKRIA+ IQKKACNALLLKVNQIGSVTESIQAALD
Sbjct: 361 DWSSWASLQSSVDIQLVGDDLLVTNPKRIAKGIQKKACNALLLKVNQIGSVTESIQAALD 420

Query: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGS 480
           SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEE+GS
Sbjct: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEEIGS 480

Query: 481 VRYAGASFRSP 492
           +RYAGASFRSP
Sbjct: 481 IRYAGASFRSP 490

BLAST of Lsi06G013690 vs. NCBI nr
Match: XP_008453338.1 (PREDICTED: enolase 1, chloroplastic [Cucumis melo] >KAA0058034.1 enolase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 909.1 bits (2348), Expect = 1.7e-260
Identity = 473/496 (95.36%), Postives = 475/496 (95.77%), Query Frame = 0

Query: 1   MASLPSPPTMNLLEKSFLSSTSQSFITSIP-----SSSSSSSKPLGSHAIRCSVAVAPSA 60
           MASLPSPPTMNLL K    S  QSFI SIP     SSSSSSSKPL S  IRCSVAVAPS 
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STKVSKEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           ST VSKEFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180
           TVYGGKGVLTAVKNI DILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNIKDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE IQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 492
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of Lsi06G013690 vs. NCBI nr
Match: TYK28380.1 (enolase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 908.7 bits (2347), Expect = 2.2e-260
Identity = 473/496 (95.36%), Postives = 475/496 (95.77%), Query Frame = 0

Query: 1   MASLPSPPTMNLLEKSFLSSTSQSFITSIP-----SSSSSSSKPLGSHAIRCSVAVAPSA 60
           MASLPSPPTMNLL K    S  QSFI SIP     SSSSSSSKPL S  IRCSVAVAPS 
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STKVSKEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           ST VSKEFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180
           TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFTK GKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE IQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 492
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of Lsi06G013690 vs. NCBI nr
Match: XP_004138202.1 (enolase 1, chloroplastic [Cucumis sativus] >KGN63743.1 hypothetical protein Csa_014309 [Cucumis sativus])

HSP 1 Score: 907.5 bits (2344), Expect = 4.9e-260
Identity = 472/492 (95.93%), Postives = 475/492 (96.54%), Query Frame = 0

Query: 1   MASLPSPPTMNLLEKSFLSSTSQSFITSIP-SSSSSSSKPLGSHAIRCSVAVAPSASTKV 60
           MASLPSP TMNLL KS   S  QSFI  IP SSSSSSSKPL S  IRCSVAVAPS ST V
Sbjct: 1   MASLPSPHTMNLLNKSLSLSPPQSFIALIPSSSSSSSSKPLRSPTIRCSVAVAPSVSTNV 60

Query: 61  SKEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 120
           SKEFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG
Sbjct: 61  SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 120

Query: 121 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCR 180
           GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKS+LGANAILGVSLSVCR
Sbjct: 121 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR 180

Query: 181 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAE 240
           AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+SFAE
Sbjct: 181 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 240

Query: 241 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 300
           ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI
Sbjct: 241 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 300

Query: 301 KIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 360
           KIGMDVAASEFFT EGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ
Sbjct: 301 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 360

Query: 361 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAAL 420
           DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE IQKKACNALLLKVNQIGSVTESIQAAL
Sbjct: 361 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL 420

Query: 421 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 480
           DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG
Sbjct: 421 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 480

Query: 481 SVRYAGASFRSP 492
           SVRYAGASFRSP
Sbjct: 481 SVRYAGASFRSP 492

BLAST of Lsi06G013690 vs. NCBI nr
Match: XP_022134829.1 (enolase 1, chloroplastic [Momordica charantia])

HSP 1 Score: 891.0 bits (2301), Expect = 4.8e-255
Identity = 461/496 (92.94%), Postives = 471/496 (94.96%), Query Frame = 0

Query: 1   MASLPSPPTMNLLEKSFLSSTSQSFITSIPSSSSSSSKP-----LGSHAIRCSVAVAPSA 60
           MASL SPP MNLL  + L S S S I SIPSSSSSSS       L S A+RCSVAVAPSA
Sbjct: 1   MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSA 60

Query: 61  STKVSKEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           +TK SKE+KLKSVKARQIIDSRGNPTVEVDL+TDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  ATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180
           +VYGGKGVLTAVKNINDILAPKLVGVDVRNQE+VDAIMLEIDGT NKSKLGANAILGVSL
Sbjct: 121 SVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYH LKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFTK+GKYDLNFKKQPNDGAHVHSAHGLGELYKQF+KDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSW+SLQSSVDIQLVGDDLLVTNPKRIAEAI KKAC ALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACTALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 492
           EELGSVRYAG SFRSP
Sbjct: 481 EELGSVRYAGESFRSP 496

BLAST of Lsi06G013690 vs. TAIR 10
Match: AT1G74030.1 (enolase 1 )

HSP 1 Score: 788.9 bits (2036), Expect = 2.4e-228
Identity = 401/482 (83.20%), Postives = 439/482 (91.08%), Query Frame = 0

Query: 13  LEKSFLSS---TSQSFITSIPSSSSSSSKPLGSHAIRCSVAVAPSASTKVSKEFKLKSVK 72
           L++SFLS    + + ++ S PS             ++CSV         V+KE ++K VK
Sbjct: 10  LQRSFLSPSRVSGERYLESAPSCLR-----FRRSGVQCSV---------VAKECRVKGVK 69

Query: 73  ARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYGGKGVLTAVKN 132
           ARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK+VYGGKGVL A+KN
Sbjct: 70  ARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLQAIKN 129

Query: 133 INDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPL 192
           IN+++APKL+GVDVRNQ +VDA+MLE+DGTPNKSKLGANAILGVSLSVCRAGAGAKG+PL
Sbjct: 130 INELVAPKLIGVDVRNQADVDALMLELDGTPNKSKLGANAILGVSLSVCRAGAGAKGVPL 189

Query: 193 YRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEALRMGSEVYH 252
           Y+HIQE SGTKELVMPVPAFNVINGGSHAGN+LAMQEFMILPVGATSF+EA +MGSEVYH
Sbjct: 190 YKHIQETSGTKELVMPVPAFNVINGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSEVYH 249

Query: 253 TLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASE 312
           TLKGIIK KYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASE
Sbjct: 250 TLKGIIKTKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASE 309

Query: 313 FFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQ 372
           FF K+G+YDLNFKKQPNDGAHV SA  L +LY++F+KDFPIVSIEDPFDQDDWSSWASLQ
Sbjct: 310 FFMKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFDQDDWSSWASLQ 369

Query: 373 SSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVM 432
           SSVDIQLVGDDLLVTNPKRIAEAI+K++CNALLLKVNQIG+VTESIQAALDSKAAGWGVM
Sbjct: 370 SSVDIQLVGDDLLVTNPKRIAEAIKKQSCNALLLKVNQIGTVTESIQAALDSKAAGWGVM 429

Query: 433 VSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFR 492
           VSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERL+KYNQLLRIEEELG+VRYAG +FR
Sbjct: 430 VSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLSKYNQLLRIEEELGNVRYAGEAFR 477

BLAST of Lsi06G013690 vs. TAIR 10
Match: AT2G36530.1 (Enolase )

HSP 1 Score: 578.2 bits (1489), Expect = 6.4e-165
Identity = 298/435 (68.51%), Postives = 350/435 (80.46%), Query Frame = 0

Query: 68  VKARQIIDSRGNPTVEVDLITDDLYR--SAVPSGASTGIYEALELRDGDKTVYGGKGVLT 127
           VKARQI DSRGNPTVEVD+ T +  +  +AVPSGASTGIYEALELRDG    Y GKGV  
Sbjct: 7   VKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGGSD-YLGKGVSK 66

Query: 128 AVKNINDILAPKLVGVDVRNQEEVDAIML-EIDGTPN-----KSKLGANAILGVSLSVCR 187
           AV N+N+I+ P L+G D   Q  +D  M+ E+DGT N     K KLGANAIL VSL+VC+
Sbjct: 67  AVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVSLAVCK 126

Query: 188 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAE 247
           AGA   G+PLY+HI  L+G  ++V+PVPAFNVINGGSHAGN LAMQEFMILPVGA SF E
Sbjct: 127 AGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAASFKE 186

Query: 248 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 307
           A++MG EVYH LK +IK KYGQDA NVGDEGGFAPN+Q+N+EGL LL  AIEKAGYTGK+
Sbjct: 187 AMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGYTGKV 246

Query: 308 KIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 367
            IGMDVAASEF++++  YDLNFK++ N+G+   S   L +LYK FV ++PIVSIEDPFDQ
Sbjct: 247 VIGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIEDPFDQ 306

Query: 368 DDWSSWASL--QSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQA 427
           DDW  +A +  +   ++Q+VGDDLLVTNPKR+A+AI +K+CNALLLKVNQIGSVTESI+A
Sbjct: 307 DDWEHYAKMTTECGTEVQIVGDDLLVTNPKRVAKAIAEKSCNALLLKVNQIGSVTESIEA 366

Query: 428 ALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEE 487
              SK AGWGVM SHRSGETED FIADL+VGL++GQIKTGAPCRSERLAKYNQLLRIEEE
Sbjct: 367 VKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLLRIEEE 426

Query: 488 LGS-VRYAGASFRSP 492
           LGS   YAG +FR P
Sbjct: 427 LGSEAIYAGVNFRKP 440

BLAST of Lsi06G013690 vs. TAIR 10
Match: AT2G29560.1 (cytosolic enolase )

HSP 1 Score: 462.2 bits (1188), Expect = 5.1e-130
Identity = 233/427 (54.57%), Postives = 308/427 (72.13%), Query Frame = 0

Query: 65  LKSVKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKTVYGGKGV 124
           +  VKARQI+DSRG PTVEVDL T+  ++R++VPSG S+G YEA+ELRDGDK +Y G  V
Sbjct: 47  ITKVKARQILDSRGIPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSV 106

Query: 125 LTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCRAGAG 184
             AVKNIN+ ++  L+G+D + Q ++D  M+++D T  KS+LGANAIL VS++ C+AGA 
Sbjct: 107 AKAVKNINEKISEALIGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAA 166

Query: 185 AKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEALRM 244
            K +PL +H+ +LSG   +V+PVPAF V++GG HA N  A+QE MILP+GA+ F EAL+ 
Sbjct: 167 EKEVPLCKHLSDLSGRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGASRFEEALQW 226

Query: 245 GSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGM 304
           GSE YH LK +I  K G   CNVG++GG AP++   +EGL L+ +AI + GY  KIKI +
Sbjct: 227 GSETYHHLKAVISEKNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTGYNDKIKIAI 286

Query: 305 DVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDDWS 364
           D+AA+ F     KYDL+ K     G +  SA  + ++YK+   D+PIVSIEDPFD++DW 
Sbjct: 287 DIAATNFCLGT-KYDLDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWE 346

Query: 365 SWASLQSSVDIQLVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALDSKA 424
                 S    Q+VGDDLL++N KR+  AIQ+ +CNALLLKVNQIG+VTE+I+    ++ 
Sbjct: 347 HTKYFSSLGICQVVGDDLLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARD 406

Query: 425 AGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG-SVR 484
           A WGV+ SHR GETED+FI+DLSVGLA+G IK GAPCR ER  KYNQLLRIEEELG    
Sbjct: 407 AQWGVVTSHRCGETEDSFISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAV 466

Query: 485 YAGASFR 490
           YAG  ++
Sbjct: 467 YAGEDWK 472

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9C43.4e-22783.20Enolase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ENO1 PE=1 SV=1[more]
P150071.7e-17068.41Enolase OS=Drosophila melanogaster OX=7227 GN=Eno PE=1 SV=2[more]
Q54RK53.8e-17070.07Enolase A OS=Dictyostelium discoideum OX=44689 GN=enoA PE=3 SV=1[more]
P428944.2e-16970.28Enolase OS=Neocallimastix frontalis OX=4757 PE=3 SV=1[more]
P154291.6e-16870.30Beta-enolase OS=Rattus norvegicus OX=10116 GN=Eno3 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A5A7US848.2e-26195.36Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A1S3BX568.2e-26195.36Phosphopyruvate hydratase OS=Cucumis melo OX=3656 GN=LOC103494088 PE=3 SV=1[more]
A0A5D3DYS61.1e-26095.36Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A0A0LPK62.4e-26095.93Phosphopyruvate hydratase OS=Cucumis sativus OX=3659 GN=Csa_1G014400 PE=3 SV=1[more]
A0A6J1BZW12.3e-25592.94Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111007005 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_038879680.15.1e-26596.95enolase 1, chloroplastic [Benincasa hispida][more]
XP_008453338.11.7e-26095.36PREDICTED: enolase 1, chloroplastic [Cucumis melo] >KAA0058034.1 enolase 1 [Cucu... [more]
TYK28380.12.2e-26095.36enolase 1 [Cucumis melo var. makuwa][more]
XP_004138202.14.9e-26095.93enolase 1, chloroplastic [Cucumis sativus] >KGN63743.1 hypothetical protein Csa_... [more]
XP_022134829.14.8e-25592.94enolase 1, chloroplastic [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G74030.12.4e-22883.20enolase 1 [more]
AT2G36530.16.4e-16568.51Enolase [more]
AT2G29560.15.1e-13054.57cytosolic enolase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000941EnolasePRINTSPR00148ENOLASEcoord: 166..182
score: 76.74
coord: 223..236
score: 75.87
coord: 401..415
score: 80.2
coord: 378..389
score: 78.16
coord: 96..110
score: 88.38
coord: 430..447
score: 83.0
IPR000941EnolaseTIGRFAMTIGR01060TIGR01060coord: 66..487
e-value: 1.9E-166
score: 551.9
IPR000941EnolaseSFLDSFLDF00002enolasecoord: 66..478
e-value: 0.0
score: 626.0
IPR000941EnolasePANTHERPTHR11902ENOLASEcoord: 29..491
IPR000941EnolaseHAMAPMF_00318Enolasecoord: 63..478
score: 43.630325
IPR000941EnolaseCDDcd03313enolasecoord: 67..477
e-value: 0.0
score: 745.844
IPR020811Enolase, N-terminalSMARTSM01193Enolase_N_3coord: 65..193
e-value: 6.7E-86
score: 301.4
IPR020811Enolase, N-terminalPFAMPF03952Enolase_Ncoord: 66..193
e-value: 4.2E-56
score: 188.8
IPR020810Enolase, C-terminal TIM barrel domainSMARTSM01192Enolase_C_3coord: 201..491
e-value: 1.9E-193
score: 658.6
IPR020810Enolase, C-terminal TIM barrel domainPFAMPF00113Enolase_Ccoord: 202..489
e-value: 2.1E-147
score: 490.1
IPR036849Enolase-like, C-terminal domain superfamilyGENE3D3.20.20.120coord: 187..491
e-value: 1.2E-152
score: 509.4
IPR036849Enolase-like, C-terminal domain superfamilySUPERFAMILY51604Enolase C-terminal domain-likecoord: 201..484
IPR029017Enolase-like, N-terminalGENE3D3.30.390.10coord: 59..186
e-value: 3.4E-56
score: 190.9
IPR029017Enolase-like, N-terminalSUPERFAMILY54826Enolase N-terminal domain-likecoord: 65..196
NoneNo IPR availableSFLDSFLDG00178enolasecoord: 66..478
e-value: 0.0
score: 626.0
NoneNo IPR availablePANTHERPTHR11902:SF42ENOLASE 1, CHLOROPLASTICcoord: 29..491
IPR020809Enolase, conserved sitePROSITEPS00164ENOLASEcoord: 401..414

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G013690.1Lsi06G013690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
biological_process GO:0010090 trichome morphogenesis
cellular_component GO:0009507 chloroplast
cellular_component GO:0000015 phosphopyruvate hydratase complex
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004634 phosphopyruvate hydratase activity