Lsi06G013600 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G013600
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	protein MIZU-KUSSEI 1-like
Location	chr06: 24171038 .. 24172557 (-)
RNA-Seq Expression	Lsi06G013600
Synteny	Lsi06G013600

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCTCTCTCTCTCCCCCCCAATATACTATCTCAAATGGTAGATATATTTCTCACCTTTCAGAAGCCGGCTTTGCCTTGGCCTTTTGGATTTACTGACACTGCAACTGCATGACTTTCTTAAACCTTCAATACTTAAATGACGGTTTTTTGTCTTTGGAGGAATCAGAAGATGGGTCTCGAGAGAGTGACTATTAGATATTTTTGTTGTATATGACATAATGTAGACTTTATGCTCAACAACGTAGGTTTATGCGTTTGCTCAGTGCCCCAACAAGCTTTGTGCGTCATTCTCTTACATGGATTTCGATTTGATTTTCGTTAGATTGTCTATTGTGAACAGCTACGGCTTATACTTAGGGTGGCTTTTGAAGCCATCGTTTAACTGTTTGTGATTCAAGTCAACCAAATGCCAATCTCACAAGTAAGTAACACAGACATTGTCTCTCTCTCTCTCTCTTTCTTTTTAATTTGAAGTCTCTCTCTCTTATGTCTCCCCGTATCAGTTGGTGGGTCAGTCTATGTTTCTTTTGTCAGGATTCTCTTGCAGCACATCAATGAACCTTGAAACTAAGGATTAAATGATGAGAGCCCCACATGGCAAATAAAAGGGGAGAAACCACATTCTCTCTATTATACACTCCTCTCATTCTCTCTCCTACCAACCAACCTTCTAAAGAATCTACCTTACTTCCTTCCCCCTTGTTTCTCAGCTGCGGACAGTAATGGCTCTGGAATCCTCCTCCAAAAGGCACTTCCATTGGACAAGGAAGGTTAGTAATGAAGATGAAGATGATTCCGCCTTCAAATCCTCAGAATCTGCCGCAGAAGATAAGCAGGAGAACTTCAAGTCCATTTCACAGGAGCCAGAGGAACAGCAGCAGGTTCGAGCACAATTGACCAGGAGGAAACTGCAACAGCTTGCCGTCTTTCGGTTCCGTTCGGTTCTCACCGCCCTCGGCCGCAACCGCAACCCACAGCTTGGTCTCCGTTCTCGAGTTGTCGGAACACTATTTGGTTCTCGCCGGGGGCATGTTCATTTTGCATTCCAGAGAGATCCCAATTCACAACCAGCTTTCTTGATTGAGCTTGCTACTCCAATCAGTGGTCTGGTTAAGGAAATGGCATCTGGATTGGTTCGAATTGCGCTGGAATGTGATAAAGAGAAAAATGAGAACAAGAAACCAGGAAGGAGACTGCTTGAAGAGCCTGTTTGGAGGACGTTTTGCAATGGGAAGAAATGTGGGTTTGCTTCAAGGAGAGAATGTGGACCTAAGGAGTTGAAGATCTTGAAGGCTGTGGAACCAATTTCAATGGGCGCTGGCGTTTTACCGGTAAACGAAGGCACCGAGGGCAGTGGCGGTTGCAGTGGGGAAGGAGAAGGTGGATCAGATGGTGAGATCATGTACATGAGGGCAAAGTTTGAGAGAATTGTAGGCTCAAGGGATTCTGAAGCTTTCTACATGATGAACCCAGACAGTAATGGAGCTCCTGAGCTCAGTATCTATTTGCTAAGAGTATAA

mRNA sequence

TCCTCTCTCTCTCCCCCCCAATATACTATCTCAAATGGTAGATATATTTCTCACCTTTCAGAAGCCGGCTTTGCCTTGGCCTTTTGGATTTACTGACACTGCAACTGCATGACTTTCTTAAACCTTCAATACTTAAATGACGGTTTTTTGTCTTTGGAGGAATCAGAAGATGGGTCTCGAGAGAGTGACTATTAGATATTTTTGTTGTATATGACATAATGTAGACTTTATGCTCAACAACGTAGGTTTATGCGTTTGCTCAGTGCCCCAACAAGCTTTGTGCGTCATTCTCTTACATGGATTTCGATTTGATTTTCGTTAGATTGTCTATTGTGAACAGCTACGGCTTATACTTAGGGTGGCTTTTGAAGCCATCGTTTAACTGTTTGTGATTCAAGTCAACCAAATGCCAATCTCACAACTGCGGACAGTAATGGCTCTGGAATCCTCCTCCAAAAGGCACTTCCATTGGACAAGGAAGGTTAGTAATGAAGATGAAGATGATTCCGCCTTCAAATCCTCAGAATCTGCCGCAGAAGATAAGCAGGAGAACTTCAAGTCCATTTCACAGGAGCCAGAGGAACAGCAGCAGGTTCGAGCACAATTGACCAGGAGGAAACTGCAACAGCTTGCCGTCTTTCGGTTCCGTTCGGTTCTCACCGCCCTCGGCCGCAACCGCAACCCACAGCTTGGTCTCCGTTCTCGAGTTGTCGGAACACTATTTGGTTCTCGCCGGGGGCATGTTCATTTTGCATTCCAGAGAGATCCCAATTCACAACCAGCTTTCTTGATTGAGCTTGCTACTCCAATCAGTGGTCTGGTTAAGGAAATGGCATCTGGATTGGTTCGAATTGCGCTGGAATGTGATAAAGAGAAAAATGAGAACAAGAAACCAGGAAGGAGACTGCTTGAAGAGCCTGTTTGGAGGACGTTTTGCAATGGGAAGAAATGTGGGTTTGCTTCAAGGAGAGAATGTGGACCTAAGGAGTTGAAGATCTTGAAGGCTGTGGAACCAATTTCAATGGGCGCTGGCGTTTTACCGGTAAACGAAGGCACCGAGGGCAGTGGCGGTTGCAGTGGGGAAGGAGAAGGTGGATCAGATGGTGAGATCATGTACATGAGGGCAAAGTTTGAGAGAATTGTAGGCTCAAGGGATTCTGAAGCTTTCTACATGATGAACCCAGACAGTAATGGAGCTCCTGAGCTCAGTATCTATTTGCTAAGAGTATAA

Coding sequence (CDS)

ATGCCAATCTCACAACTGCGGACAGTAATGGCTCTGGAATCCTCCTCCAAAAGGCACTTCCATTGGACAAGGAAGGTTAGTAATGAAGATGAAGATGATTCCGCCTTCAAATCCTCAGAATCTGCCGCAGAAGATAAGCAGGAGAACTTCAAGTCCATTTCACAGGAGCCAGAGGAACAGCAGCAGGTTCGAGCACAATTGACCAGGAGGAAACTGCAACAGCTTGCCGTCTTTCGGTTCCGTTCGGTTCTCACCGCCCTCGGCCGCAACCGCAACCCACAGCTTGGTCTCCGTTCTCGAGTTGTCGGAACACTATTTGGTTCTCGCCGGGGGCATGTTCATTTTGCATTCCAGAGAGATCCCAATTCACAACCAGCTTTCTTGATTGAGCTTGCTACTCCAATCAGTGGTCTGGTTAAGGAAATGGCATCTGGATTGGTTCGAATTGCGCTGGAATGTGATAAAGAGAAAAATGAGAACAAGAAACCAGGAAGGAGACTGCTTGAAGAGCCTGTTTGGAGGACGTTTTGCAATGGGAAGAAATGTGGGTTTGCTTCAAGGAGAGAATGTGGACCTAAGGAGTTGAAGATCTTGAAGGCTGTGGAACCAATTTCAATGGGCGCTGGCGTTTTACCGGTAAACGAAGGCACCGAGGGCAGTGGCGGTTGCAGTGGGGAAGGAGAAGGTGGATCAGATGGTGAGATCATGTACATGAGGGCAAAGTTTGAGAGAATTGTAGGCTCAAGGGATTCTGAAGCTTTCTACATGATGAACCCAGACAGTAATGGAGCTCCTGAGCTCAGTATCTATTTGCTAAGAGTATAA

Protein sequence

MPISQLRTVMALESSSKRHFHWTRKVSNEDEDDSAFKSSESAAEDKQENFKSISQEPEEQQQVRAQLTRRKLQQLAVFRFRSVLTALGRNRNPQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSQPAFLIELATPISGLVKEMASGLVRIALECDKEKNENKKPGRRLLEEPVWRTFCNGKKCGFASRRECGPKELKILKAVEPISMGAGVLPVNEGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELSIYLLRV

Homology

[Close]

BLAST of Lsi06G013600 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 2.8e-45
Identity = 93/180 (51.67%), Postives = 122/180 (67.78%), Query Frame = 0


Query: 97  LRSRVVGTLFGSRRGHVHFAFQRDPNSQPAFLIELATPISGLVKEMASGLVRIALECDKE 156
           L  RV GTL+G +RGHV F+ Q +  S P  L++LA   + LVKEM+SGLVRIALEC+K 
Sbjct: 125 LGRRVTGTLYGHKRGHVTFSVQYNQRSDPVLLLDLAMSTATLVKEMSSGLVRIALECEK- 184

Query: 157 KNENKKPGRRLLEEPVWRTFCNGKKCGFASRR--ECGPKELKILKAVEPISMGAGVLPVN 216
                + G +L +EP W  +CNG+KCG+A  R   C   + ++L  V  +++GAGV+P  
Sbjct: 185 ---RHRSGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTP 244

Query: 217 EGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELSIYLLRV 275
           +  +     SG G G   GE++YMR KFER+VGSRDSEAFYMMNPD NG PELSI+LLR+
Sbjct: 245 KTID---DVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFLLRI 297

[Close]

BLAST of Lsi06G013600 vs. ExPASy TrEMBL
Match: A0A6J1E1K6 (protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111430002 PE=4 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 3.2e-129
Identity = 244/265 (92.08%), Postives = 253/265 (95.47%), Query Frame = 0


Query: 10  MALESSSKRHFHWTRKVSNEDEDDSAFKSSESAAEDKQENFKSISQEPEEQQQVRAQLTR 69
           MA+E SSKRHFHWTRKVSNE EDDS FKSS+SA E+KQEN KSI QEP+EQQQ RAQLTR
Sbjct: 1   MAMEFSSKRHFHWTRKVSNE-EDDSTFKSSDSATEEKQENAKSILQEPQEQQQFRAQLTR 60

Query: 70  RKLQQLAVFRFRSVLTALGRNRNPQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSQPAFLI 129
           RKLQQLAV RFRSVLTALGRNRNPQLGLRSRV+GTLFGSRRGHVHFAFQRDP+S PAFLI
Sbjct: 61  RKLQQLAVSRFRSVLTALGRNRNPQLGLRSRVIGTLFGSRRGHVHFAFQRDPSSHPAFLI 120

Query: 130 ELATPISGLVKEMASGLVRIALECDKEKNENKKPGRRLLEEPVWRTFCNGKKCGFASRRE 189
           ELATPISGLVKEMASGLVRIALECDKEK+ NKKPG RLLEEPVWRTFCNGKKCGFASRRE
Sbjct: 121 ELATPISGLVKEMASGLVRIALECDKEKDVNKKPGGRLLEEPVWRTFCNGKKCGFASRRE 180

Query: 190 CGPKELKILKAVEPISMGAGVLPVNEGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGSR 249
           CGPKELKILKAVEPISMGAGVLPV+EGTEGSGG SGEGEGGSDG+IMYMRAKFERIVGSR
Sbjct: 181 CGPKELKILKAVEPISMGAGVLPVDEGTEGSGGGSGEGEGGSDGDIMYMRAKFERIVGSR 240

Query: 250 DSEAFYMMNPDSNGAPELSIYLLRV 275
           DSEAFYMMNPDSNGAPELSIYLLRV
Sbjct: 241 DSEAFYMMNPDSNGAPELSIYLLRV 264

[Close]

BLAST of Lsi06G013600 vs. ExPASy TrEMBL
Match: A0A6J1JA11 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111484901 PE=4 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 5.4e-129
Identity = 244/266 (91.73%), Postives = 254/266 (95.49%), Query Frame = 0


Query: 10  MALESSSKRHFHWTRKVSNEDEDDSAFKSSESAAEDKQENFKSISQEPEEQQQVRAQLTR 69
           MALE SS+RHFHWTRKVSNE+EDDS FKSS+SA E+KQEN KSI QEP+EQQQVRAQLTR
Sbjct: 1   MALEFSSRRHFHWTRKVSNEEEDDSTFKSSDSAKEEKQENAKSILQEPQEQQQVRAQLTR 60

Query: 70  RKLQQLAVFRFRSVLTALGRNRNPQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSQPAFLI 129
           RKLQQLAV RFRSVLTALGRNRNPQLGLRSRV+GTLFGSRRGHVHFAFQRDP+S PAFLI
Sbjct: 61  RKLQQLAVSRFRSVLTALGRNRNPQLGLRSRVIGTLFGSRRGHVHFAFQRDPSSHPAFLI 120

Query: 130 ELATPISGLVKEMASGLVRIALECDKEKNENKKPGRRLLEEPVWRTFCNGKKCGFASRRE 189
           ELATPISGLVKEMASGLVRIALECDKEK+ NKKPG RLLEEPVWRTFCNGKKCGFASRRE
Sbjct: 121 ELATPISGLVKEMASGLVRIALECDKEKDVNKKPGGRLLEEPVWRTFCNGKKCGFASRRE 180

Query: 190 CGPKELKILKAVEPISMGAGVLPV-NEGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGS 249
           CGPKELKILKAVEPISMGAGVLPV +EGTEGS G SGEGEGGSDG+IMYMRAKFERIVGS
Sbjct: 181 CGPKELKILKAVEPISMGAGVLPVEDEGTEGSSGGSGEGEGGSDGDIMYMRAKFERIVGS 240

Query: 250 RDSEAFYMMNPDSNGAPELSIYLLRV 275
           RDSEAFYMMNPDSNGAPELSIYLLRV
Sbjct: 241 RDSEAFYMMNPDSNGAPELSIYLLRV 266

[Close]

BLAST of Lsi06G013600 vs. ExPASy TrEMBL
Match: A0A5D3DX17 (Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00150 PE=4 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.6e-128
Identity = 245/266 (92.11%), Postives = 249/266 (93.61%), Query Frame = 0


Query: 10  MALESSSKRHFHWTRKVSNEDEDDSAFKSSESAAEDKQENFKSISQEPEEQQQVRAQLTR 69
           MALESSS+RHFHWTRKVSNEDEDDS F S ESA E KQEN K ISQEPEEQQQVRAQLTR
Sbjct: 1   MALESSSRRHFHWTRKVSNEDEDDSTFNSLESAVEKKQENAKPISQEPEEQQQVRAQLTR 60

Query: 70  RKLQQLAVFRFRSVLTALGRNRNPQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSQPAFLI 129
           RKLQQLAV RFRSVLTALGRNRN Q GLRSRVVGTLFGSRRGHVHFAFQRDPNS PAFLI
Sbjct: 61  RKLQQLAVSRFRSVLTALGRNRNSQHGLRSRVVGTLFGSRRGHVHFAFQRDPNSNPAFLI 120

Query: 130 ELATPISGLVKEMASGLVRIALECDKEKNENKK-PGRRLLEEPVWRTFCNGKKCGFASRR 189
           ELATPISGLVKEMASGLVRIALECDKEK+ENKK PGRRLLEEPVWRTFCNGKKCGFASRR
Sbjct: 121 ELATPISGLVKEMASGLVRIALECDKEKDENKKPPGRRLLEEPVWRTFCNGKKCGFASRR 180

Query: 190 ECGPKELKILKAVEPISMGAGVLPVNEGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGS 249
           ECGPKELKILKAVEPISMGAGVLPVNE  +G  GCS EGEGGSDG+IMYMRAKFERIVGS
Sbjct: 181 ECGPKELKILKAVEPISMGAGVLPVNEDAKGCDGCSTEGEGGSDGDIMYMRAKFERIVGS 240

Query: 250 RDSEAFYMMNPDSNGAPELSIYLLRV 275
           RDSEAFYMMNPDSNGAPELSIYLLRV
Sbjct: 241 RDSEAFYMMNPDSNGAPELSIYLLRV 266

[Close]

BLAST of Lsi06G013600 vs. ExPASy TrEMBL
Match: A0A1S3BVG6 (protein MIZU-KUSSEI 1 OS=Cucumis melo OX=3656 GN=LOC103494096 PE=4 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.6e-128
Identity = 245/266 (92.11%), Postives = 249/266 (93.61%), Query Frame = 0


Query: 10  MALESSSKRHFHWTRKVSNEDEDDSAFKSSESAAEDKQENFKSISQEPEEQQQVRAQLTR 69
           MALESSS+RHFHWTRKVSNEDEDDS F S ESA E KQEN K ISQEPEEQQQVRAQLTR
Sbjct: 1   MALESSSRRHFHWTRKVSNEDEDDSTFNSLESAVEKKQENAKPISQEPEEQQQVRAQLTR 60

Query: 70  RKLQQLAVFRFRSVLTALGRNRNPQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSQPAFLI 129
           RKLQQLAV RFRSVLTALGRNRN Q GLRSRVVGTLFGSRRGHVHFAFQRDPNS PAFLI
Sbjct: 61  RKLQQLAVSRFRSVLTALGRNRNSQHGLRSRVVGTLFGSRRGHVHFAFQRDPNSNPAFLI 120

Query: 130 ELATPISGLVKEMASGLVRIALECDKEKNENKK-PGRRLLEEPVWRTFCNGKKCGFASRR 189
           ELATPISGLVKEMASGLVRIALECDKEK+ENKK PGRRLLEEPVWRTFCNGKKCGFASRR
Sbjct: 121 ELATPISGLVKEMASGLVRIALECDKEKDENKKPPGRRLLEEPVWRTFCNGKKCGFASRR 180

Query: 190 ECGPKELKILKAVEPISMGAGVLPVNEGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGS 249
           ECGPKELKILKAVEPISMGAGVLPVNE  +G  GCS EGEGGSDG+IMYMRAKFERIVGS
Sbjct: 181 ECGPKELKILKAVEPISMGAGVLPVNEDAKGCDGCSTEGEGGSDGDIMYMRAKFERIVGS 240

Query: 250 RDSEAFYMMNPDSNGAPELSIYLLRV 275
           RDSEAFYMMNPDSNGAPELSIYLLRV
Sbjct: 241 RDSEAFYMMNPDSNGAPELSIYLLRV 266

[Close]

BLAST of Lsi06G013600 vs. ExPASy TrEMBL
Match: A0A6J1KGY0 (uncharacterized protein LOC111495163 OS=Cucurbita maxima OX=3661 GN=LOC111495163 PE=4 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 1.0e-127
Identity = 242/273 (88.64%), Postives = 254/273 (93.04%), Query Frame = 0


Query: 3   ISQLRTVMALESSSKRHFHWTRKVSNEDEDDSAFKSSESAAEDK-QENFKSISQEPEEQQ 62
           +SQL+ VMALESSS+RHFHWTRKVSNEDEDDS FKSSESA E+K +EN K ISQ+ + QQ
Sbjct: 125 VSQLQPVMALESSSRRHFHWTRKVSNEDEDDSTFKSSESAPEEKEEENAKPISQDSQHQQ 184

Query: 63  QVRAQLTRRKLQQLAVFRFRSVLTALGRNRNPQLGLRSRVVGTLFGSRRGHVHFAFQRDP 122
           QV AQL+RRKLQQLAV RFRSVLTALGR+RN QLGLRSRVVGTLFGSRRGHVHFAFQRDP
Sbjct: 185 QVGAQLSRRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDP 244

Query: 123 NSQPAFLIELATPISGLVKEMASGLVRIALECDKEKNENKKPGRRLLEEPVWRTFCNGKK 182
           NS PAFLIELATPISGLVKEMASGLVRIALECDKEK ENKKPGRRLLEEP+WRTFCNGKK
Sbjct: 245 NSHPAFLIELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKK 304

Query: 183 CGFASRRECGPKELKILKAVEPISMGAGVLPVNEGTEGSGGCSGEGEGGSDGEIMYMRAK 242
           CGFASRRECGPKE KILKAVEPISMGAGVLPVNEGTEG G   GEG+ GSDGEIMYMRAK
Sbjct: 305 CGFASRRECGPKEWKILKAVEPISMGAGVLPVNEGTEGCGSGGGEGKEGSDGEIMYMRAK 364

Query: 243 FERIVGSRDSEAFYMMNPDSNGAPELSIYLLRV 275
           FERIVGSRDSEAFYMMNPDSNGAPELS+YLLRV
Sbjct: 365 FERIVGSRDSEAFYMMNPDSNGAPELSVYLLRV 397

[Close]

BLAST of Lsi06G013600 vs. NCBI nr
Match: XP_038879125.1 (protein MIZU-KUSSEI 1 [Benincasa hispida])

HSP 1 Score: 483.0 bits (1242), Expect = 1.7e-132
Identity = 248/265 (93.58%), Postives = 254/265 (95.85%), Query Frame = 0


Query: 10  MALESSSKRHFHWTRKVSNEDEDDSAFKSSESAAEDKQENFKSISQEPEEQQQVRAQLTR 69
           MALESSS+RHFHWTRKVSNEDEDDS F SSESA E K+EN K ISQEPEEQQQVRAQLTR
Sbjct: 1   MALESSSRRHFHWTRKVSNEDEDDSTFNSSESAVEQKEENAKPISQEPEEQQQVRAQLTR 60

Query: 70  RKLQQLAVFRFRSVLTALGRNRNPQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSQPAFLI 129
           RKLQQLAV RFRSVLTALGRNRN +  LRSRVVGTLFGSR+GHVHFAFQRDPNS PAFLI
Sbjct: 61  RKLQQLAVSRFRSVLTALGRNRNTRHSLRSRVVGTLFGSRQGHVHFAFQRDPNSHPAFLI 120

Query: 130 ELATPISGLVKEMASGLVRIALECDKEKNENKKPGRRLLEEPVWRTFCNGKKCGFASRRE 189
           ELATPISGLVKEMASGLVRIALECDKEK+ENKKPGRRLLEEPVWRTFCNGKKCGFASRRE
Sbjct: 121 ELATPISGLVKEMASGLVRIALECDKEKDENKKPGRRLLEEPVWRTFCNGKKCGFASRRE 180

Query: 190 CGPKELKILKAVEPISMGAGVLPVNEGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGSR 249
           CGPKELKILKAVEPISMGAGVLPVN+GTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGSR
Sbjct: 181 CGPKELKILKAVEPISMGAGVLPVNKGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGSR 240

Query: 250 DSEAFYMMNPDSNGAPELSIYLLRV 275
           DSEAFYMMNPDSNGAPELSIYLLRV
Sbjct: 241 DSEAFYMMNPDSNGAPELSIYLLRV 265

[Close]

BLAST of Lsi06G013600 vs. NCBI nr
Match: XP_023516545.1 (protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 478.0 bits (1229), Expect = 5.4e-131
Identity = 245/265 (92.45%), Postives = 255/265 (96.23%), Query Frame = 0


Query: 10  MALESSSKRHFHWTRKVSNEDEDDSAFKSSESAAEDKQENFKSISQEPEEQQQVRAQLTR 69
           MA E SS+RHFHWTRKVSNE+EDDS FKSSESA E+KQEN KSI QEP+EQQQVRAQLTR
Sbjct: 1   MAQEFSSRRHFHWTRKVSNEEEDDSTFKSSESATEEKQENAKSILQEPQEQQQVRAQLTR 60

Query: 70  RKLQQLAVFRFRSVLTALGRNRNPQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSQPAFLI 129
           RKLQQLAV RFRSVLTALGRNRNPQLGLRSRV+GTLFGSRRGHVHFAFQRDP+S PAFLI
Sbjct: 61  RKLQQLAVSRFRSVLTALGRNRNPQLGLRSRVIGTLFGSRRGHVHFAFQRDPSSHPAFLI 120

Query: 130 ELATPISGLVKEMASGLVRIALECDKEKNENKKPGRRLLEEPVWRTFCNGKKCGFASRRE 189
           ELATPISGLVKEMASGLVRIALECDKEK+ NKKPGRRLLEEPVWRTFCNGKKCGFASRRE
Sbjct: 121 ELATPISGLVKEMASGLVRIALECDKEKDVNKKPGRRLLEEPVWRTFCNGKKCGFASRRE 180

Query: 190 CGPKELKILKAVEPISMGAGVLPVNEGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGSR 249
           CGPKELKILKAVEPISMGAGVLPV+EGTEG+GG SGEGEGGSDG+IMYMRAKFERIVGSR
Sbjct: 181 CGPKELKILKAVEPISMGAGVLPVDEGTEGTGGGSGEGEGGSDGDIMYMRAKFERIVGSR 240

Query: 250 DSEAFYMMNPDSNGAPELSIYLLRV 275
           DSEAFYMMNPDSNGAPELSIYLLRV
Sbjct: 241 DSEAFYMMNPDSNGAPELSIYLLRV 265

[Close]

BLAST of Lsi06G013600 vs. NCBI nr
Match: XP_022921862.1 (protein MIZU-KUSSEI 1-like [Cucurbita moschata])

HSP 1 Score: 471.1 bits (1211), Expect = 6.6e-129
Identity = 244/265 (92.08%), Postives = 253/265 (95.47%), Query Frame = 0


Query: 10  MALESSSKRHFHWTRKVSNEDEDDSAFKSSESAAEDKQENFKSISQEPEEQQQVRAQLTR 69
           MA+E SSKRHFHWTRKVSNE EDDS FKSS+SA E+KQEN KSI QEP+EQQQ RAQLTR
Sbjct: 1   MAMEFSSKRHFHWTRKVSNE-EDDSTFKSSDSATEEKQENAKSILQEPQEQQQFRAQLTR 60

Query: 70  RKLQQLAVFRFRSVLTALGRNRNPQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSQPAFLI 129
           RKLQQLAV RFRSVLTALGRNRNPQLGLRSRV+GTLFGSRRGHVHFAFQRDP+S PAFLI
Sbjct: 61  RKLQQLAVSRFRSVLTALGRNRNPQLGLRSRVIGTLFGSRRGHVHFAFQRDPSSHPAFLI 120

Query: 130 ELATPISGLVKEMASGLVRIALECDKEKNENKKPGRRLLEEPVWRTFCNGKKCGFASRRE 189
           ELATPISGLVKEMASGLVRIALECDKEK+ NKKPG RLLEEPVWRTFCNGKKCGFASRRE
Sbjct: 121 ELATPISGLVKEMASGLVRIALECDKEKDVNKKPGGRLLEEPVWRTFCNGKKCGFASRRE 180

Query: 190 CGPKELKILKAVEPISMGAGVLPVNEGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGSR 249
           CGPKELKILKAVEPISMGAGVLPV+EGTEGSGG SGEGEGGSDG+IMYMRAKFERIVGSR
Sbjct: 181 CGPKELKILKAVEPISMGAGVLPVDEGTEGSGGGSGEGEGGSDGDIMYMRAKFERIVGSR 240

Query: 250 DSEAFYMMNPDSNGAPELSIYLLRV 275
           DSEAFYMMNPDSNGAPELSIYLLRV
Sbjct: 241 DSEAFYMMNPDSNGAPELSIYLLRV 264

[Close]

BLAST of Lsi06G013600 vs. NCBI nr
Match: XP_022987312.1 (protein MIZU-KUSSEI 1-like [Cucurbita maxima])

HSP 1 Score: 470.3 bits (1209), Expect = 1.1e-128
Identity = 244/266 (91.73%), Postives = 254/266 (95.49%), Query Frame = 0


Query: 10  MALESSSKRHFHWTRKVSNEDEDDSAFKSSESAAEDKQENFKSISQEPEEQQQVRAQLTR 69
           MALE SS+RHFHWTRKVSNE+EDDS FKSS+SA E+KQEN KSI QEP+EQQQVRAQLTR
Sbjct: 1   MALEFSSRRHFHWTRKVSNEEEDDSTFKSSDSAKEEKQENAKSILQEPQEQQQVRAQLTR 60

Query: 70  RKLQQLAVFRFRSVLTALGRNRNPQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSQPAFLI 129
           RKLQQLAV RFRSVLTALGRNRNPQLGLRSRV+GTLFGSRRGHVHFAFQRDP+S PAFLI
Sbjct: 61  RKLQQLAVSRFRSVLTALGRNRNPQLGLRSRVIGTLFGSRRGHVHFAFQRDPSSHPAFLI 120

Query: 130 ELATPISGLVKEMASGLVRIALECDKEKNENKKPGRRLLEEPVWRTFCNGKKCGFASRRE 189
           ELATPISGLVKEMASGLVRIALECDKEK+ NKKPG RLLEEPVWRTFCNGKKCGFASRRE
Sbjct: 121 ELATPISGLVKEMASGLVRIALECDKEKDVNKKPGGRLLEEPVWRTFCNGKKCGFASRRE 180

Query: 190 CGPKELKILKAVEPISMGAGVLPV-NEGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGS 249
           CGPKELKILKAVEPISMGAGVLPV +EGTEGS G SGEGEGGSDG+IMYMRAKFERIVGS
Sbjct: 181 CGPKELKILKAVEPISMGAGVLPVEDEGTEGSSGGSGEGEGGSDGDIMYMRAKFERIVGS 240

Query: 250 RDSEAFYMMNPDSNGAPELSIYLLRV 275
           RDSEAFYMMNPDSNGAPELSIYLLRV
Sbjct: 241 RDSEAFYMMNPDSNGAPELSIYLLRV 266

[Close]

BLAST of Lsi06G013600 vs. NCBI nr
Match: XP_008453350.1 (PREDICTED: protein MIZU-KUSSEI 1 [Cucumis melo] >KAA0058042.1 protein MIZU-KUSSEI 1 [Cucumis melo var. makuwa] >TYK28387.1 protein MIZU-KUSSEI 1 [Cucumis melo var. makuwa])

HSP 1 Score: 468.8 bits (1205), Expect = 3.3e-128
Identity = 245/266 (92.11%), Postives = 249/266 (93.61%), Query Frame = 0


Query: 10  MALESSSKRHFHWTRKVSNEDEDDSAFKSSESAAEDKQENFKSISQEPEEQQQVRAQLTR 69
           MALESSS+RHFHWTRKVSNEDEDDS F S ESA E KQEN K ISQEPEEQQQVRAQLTR
Sbjct: 1   MALESSSRRHFHWTRKVSNEDEDDSTFNSLESAVEKKQENAKPISQEPEEQQQVRAQLTR 60

Query: 70  RKLQQLAVFRFRSVLTALGRNRNPQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSQPAFLI 129
           RKLQQLAV RFRSVLTALGRNRN Q GLRSRVVGTLFGSRRGHVHFAFQRDPNS PAFLI
Sbjct: 61  RKLQQLAVSRFRSVLTALGRNRNSQHGLRSRVVGTLFGSRRGHVHFAFQRDPNSNPAFLI 120

Query: 130 ELATPISGLVKEMASGLVRIALECDKEKNENKK-PGRRLLEEPVWRTFCNGKKCGFASRR 189
           ELATPISGLVKEMASGLVRIALECDKEK+ENKK PGRRLLEEPVWRTFCNGKKCGFASRR
Sbjct: 121 ELATPISGLVKEMASGLVRIALECDKEKDENKKPPGRRLLEEPVWRTFCNGKKCGFASRR 180

Query: 190 ECGPKELKILKAVEPISMGAGVLPVNEGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGS 249
           ECGPKELKILKAVEPISMGAGVLPVNE  +G  GCS EGEGGSDG+IMYMRAKFERIVGS
Sbjct: 181 ECGPKELKILKAVEPISMGAGVLPVNEDAKGCDGCSTEGEGGSDGDIMYMRAKFERIVGS 240

Query: 250 RDSEAFYMMNPDSNGAPELSIYLLRV 275
           RDSEAFYMMNPDSNGAPELSIYLLRV
Sbjct: 241 RDSEAFYMMNPDSNGAPELSIYLLRV 266

[Close]

BLAST of Lsi06G013600 vs. TAIR 10
Match: AT5G23100.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 293.1 bits (749), Expect = 2.3e-79
Identity = 176/291 (60.48%), Postives = 196/291 (67.35%), Query Frame = 0


Query: 10  MALESSSKRH--FHWTRKVSNEDEDDSAFKSSESAAEDKQENFKSISQEPEEQQQVRAQL 69
           MA + +SKRH  FHWTRKV +++ DD     S         N K  S        V    
Sbjct: 1   MAKDFASKRHNSFHWTRKVGSDENDD----VSSHKPLPHHNNTKPSSSSSSSSSNVITH- 60

Query: 70  TRRKLQQLAVFRFRSVLTALGRNR--NPQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSQP 129
            ++KLQ  AV R RSV+  L R R  N   GL SRVVGTLFGSRRGHVHF+ Q+DPNS P
Sbjct: 61  -KKKLQSFAVSRLRSVIATLSRARPGNQNSGLGSRVVGTLFGSRRGHVHFSIQKDPNSPP 120

Query: 130 AFLIELATPISGLVKEMASGLVRIALECDK--------EKNENKKPG------------- 189
           AFLIELATPISGLVKEMASGLVRIALECDK        EKN   + G             
Sbjct: 121 AFLIELATPISGLVKEMASGLVRIALECDKGKEEEEGEEKNGTLRHGGGDKTKTTTTAAV 180

Query: 190 -RRLLEEPVWRTFCNGKKCGFASRRECGPKELKILKAVEPISMGAGVLPVNEGTEGSGGC 249
            RRL+EEP+WRT+CNGKKCGFA+RRECG KE K+LKA+E +SMGAGVLP  E   G GG 
Sbjct: 181 SRRLVEEPMWRTYCNGKKCGFATRRECGEKEKKVLKALEMVSMGAGVLPETEEIGGGGG- 240

Query: 250 SGEGEGGSDGEIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELSIYLLRV 275
                GG  G+IMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELSIYLLR+
Sbjct: 241 -----GG--GDIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELSIYLLRI 277

[Close]

BLAST of Lsi06G013600 vs. TAIR 10
Match: AT3G25640.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 233.8 bits (595), Expect = 1.6e-61
Identity = 143/271 (52.77%), Postives = 177/271 (65.31%), Query Frame = 0


Query: 14  SSSKRHFHWTRKVSNEDEDDSAFKSSESAAEDKQENFKSI------SQEPEEQQQVRAQL 73
           S SKR+F+W +K   ED+D+      E   +D   N K +      S +P    Q  AQ 
Sbjct: 15  SLSKRYFNWKKKKVQEDDDE----EEEEEEDDYNHNEKKVLTRFNFSSDPTRPDQFNAQQ 74

Query: 74  TRRKLQQLAVFRFRSVLTALGRNRNPQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSQPAF 133
             +K ++  + + R    ALG + +   GL  RVVGTLFG+RRGHV+FA Q DP   PA 
Sbjct: 75  IMKKKKKKTLEKIR---YALGFSNS---GLGFRVVGTLFGNRRGHVYFAVQDDPTRLPAV 134

Query: 134 LIELATPISGLVKEMASGLVRIALECDKEKNENKKPGRRLLEEPVWRTFCNGKKCGFASR 193
           LI+L TP S LV+EMASGLVRIALE    K ++KK   +LLEE  WRT+CNGKKCG+A+R
Sbjct: 135 LIQLPTPTSVLVREMASGLVRIALETAAYKTDSKK---KLLEESTWRTYCNGKKCGYAAR 194

Query: 194 RECGPKELKILKAVEPISMGAGVLPVNEGT---EGSGGCSGEGEGGSDGEIMYMRAKFER 253
           +ECG  E K+LKAV PI+MGAGVLP    T   EG+G       G   GE+MYMRA+FER
Sbjct: 195 KECGEAEWKVLKAVGPITMGAGVLPATTTTVDEEGNGAV-----GSEKGELMYMRARFER 254

Query: 254 IVGSRDSEAFYMMNPD-SNGAPELSIYLLRV 275
           +VGSRDSEAFYMMNPD S+G PELS+Y LRV
Sbjct: 255 VVGSRDSEAFYMMNPDVSSGGPELSVYFLRV 267

[Close]

BLAST of Lsi06G013600 vs. TAIR 10
Match: AT2G41660.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 183.7 bits (465), Expect = 2.0e-46
Identity = 93/180 (51.67%), Postives = 122/180 (67.78%), Query Frame = 0


Query: 97  LRSRVVGTLFGSRRGHVHFAFQRDPNSQPAFLIELATPISGLVKEMASGLVRIALECDKE 156
           L  RV GTL+G +RGHV F+ Q +  S P  L++LA   + LVKEM+SGLVRIALEC+K 
Sbjct: 125 LGRRVTGTLYGHKRGHVTFSVQYNQRSDPVLLLDLAMSTATLVKEMSSGLVRIALECEK- 184

Query: 157 KNENKKPGRRLLEEPVWRTFCNGKKCGFASRR--ECGPKELKILKAVEPISMGAGVLPVN 216
                + G +L +EP W  +CNG+KCG+A  R   C   + ++L  V  +++GAGV+P  
Sbjct: 185 ---RHRSGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTP 244

Query: 217 EGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELSIYLLRV 275
           +  +     SG G G   GE++YMR KFER+VGSRDSEAFYMMNPD NG PELSI+LLR+
Sbjct: 245 KTID---DVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFLLRI 297

[Close]

BLAST of Lsi06G013600 vs. TAIR 10
Match: AT5G06990.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 169.1 bits (427), Expect = 5.0e-42
Identity = 100/248 (40.32%), Postives = 151/248 (60.89%), Query Frame = 0


Query: 37  KSSESAAEDKQENFKSISQEPEEQQQVRAQLTRRKLQQLAVFRFRSVLTAL--------G 96
           KS +S A  + E   ++ Q     +   ++ T+   +  +VFR   +++ +        G
Sbjct: 23  KSKKSTATVRPE--ITLEQPSGRNKTTGSKSTKLFRRVRSVFRSLPIMSPMCKFPVGGGG 82

Query: 97  RNRNPQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSQPAFLIELATPISGLVKEMASGLVR 156
           R     +   +RV GTLFG R+  V+ A Q +P S P  L+ELA P   L++++  GLVR
Sbjct: 83  RLHENHVHGGTRVTGTLFGYRKTRVNLAVQENPRSLPILLLELAIPTGKLLQDLGVGLVR 142

Query: 157 IALECDKEKNENKKPGRRLLEEPVWRTFCNGKKCGFASRRECGPKELKILKAVEPISMGA 216
           IALEC+K+ +E  K    +++EP+W  +CNGKK G+  +R+   ++L +++ +  +SMGA
Sbjct: 143 IALECEKKPSEKTK----IIDEPIWALYCNGKKSGYGVKRQPTEEDLVVMQMLHAVSMGA 202

Query: 217 GVLPVNEG--TEGSGGCSGEGEGGSDGEIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPE 275
           GVLPV+ G  TE SGG  G   G  +G++ YMRA FER++GSRDSE +YMMNPD N  PE
Sbjct: 203 GVLPVSSGAITEQSGGGGG---GQQEGDLTYMRAHFERVIGSRDSETYYMMNPDGNSGPE 261

[Close]

BLAST of Lsi06G013600 vs. TAIR 10
Match: AT4G39610.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 166.8 bits (421), Expect = 2.5e-41
Identity = 84/194 (43.30%), Postives = 124/194 (63.92%), Query Frame = 0


Query: 92  NPQLGL------RSRVVGTLFGSRRGHVHFAFQRDPNSQPAFLIELATPISGLVKEMASG 151
           +P LGL       SR+ GTLFG R+G V  + Q +P   P+ ++ELA   + L KE+++G
Sbjct: 82  HPGLGLPDPHHNTSRITGTLFGYRKGRVSLSIQENPKCLPSLVVELAMQTTTLQKELSTG 141

Query: 152 LVRIALECDKE---KNENKKPGRR--LLEEPVWRTFCNGKKCGFASRRECGPKELKILKA 211
           +VRIALE +K+    N N K  ++  +LEEP+W  +C G+K G+  +RE   ++L +++ 
Sbjct: 142 MVRIALETEKQPRADNNNSKTEKKTDILEEPLWTMYCKGEKTGYGVKREATEEDLNVMEL 201

Query: 212 VEPISMGAGVLPVNEGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGSRDSEAFYMMNPD 271
           + P+SMGAGVLP N  +E           G DGE+ YMRA FER++GS+DSE FYM++P+
Sbjct: 202 LRPVSMGAGVLPGNSESE-----------GPDGEMAYMRAYFERVIGSKDSETFYMLSPE 261

Query: 272 SNGAPELSIYLLRV 275
            N  PELS + +RV
Sbjct: 262 GNNGPELSFFFVRV 264

The following BLAST results are available for this feature:

BLAST of Lsi06G013600 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22227	2.8e-45	51.67	Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1	[more]

BLAST of Lsi06G013600 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E1K6	3.2e-129	92.08	protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111430002 PE=4 SV...	[more]
A0A6J1JA11	5.4e-129	91.73	protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111484901 PE=4 SV=1	[more]
A0A5D3DX17	1.6e-128	92.11	Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold62...	[more]
A0A1S3BVG6	1.6e-128	92.11	protein MIZU-KUSSEI 1 OS=Cucumis melo OX=3656 GN=LOC103494096 PE=4 SV=1	[more]
A0A6J1KGY0	1.0e-127	88.64	uncharacterized protein LOC111495163 OS=Cucurbita maxima OX=3661 GN=LOC111495163...	[more]

BLAST of Lsi06G013600 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879125.1	1.7e-132	93.58	protein MIZU-KUSSEI 1 [Benincasa hispida]	[more]
XP_023516545.1	5.4e-131	92.45	protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022921862.1	6.6e-129	92.08	protein MIZU-KUSSEI 1-like [Cucurbita moschata]	[more]
XP_022987312.1	1.1e-128	91.73	protein MIZU-KUSSEI 1-like [Cucurbita maxima]	[more]
XP_008453350.1	3.3e-128	92.11	PREDICTED: protein MIZU-KUSSEI 1 [Cucumis melo] >KAA0058042.1 protein MIZU-KUSSE...	[more]

BLAST of Lsi06G013600 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23100.1	2.3e-79	60.48	Protein of unknown function, DUF617	[more]
AT3G25640.1	1.6e-61	52.77	Protein of unknown function, DUF617	[more]
AT2G41660.1	2.0e-46	51.67	Protein of unknown function, DUF617	[more]
AT5G06990.1	5.0e-42	40.32	Protein of unknown function, DUF617	[more]
AT4G39610.1	2.5e-41	43.30	Protein of unknown function, DUF617	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006460	Protein MIZU-KUSSEI 1-like, plant	PFAM	PF04759	DUF617	coord: 101..273 e-value: 4.8E-69 score: 231.6
IPR006460	Protein MIZU-KUSSEI 1-like, plant	TIGRFAM	TIGR01570	TIGR01570	coord: 101..274 e-value: 2.2E-63 score: 211.0
IPR006460	Protein MIZU-KUSSEI 1-like, plant	PANTHER	PTHR31696	PROTEIN MIZU-KUSSEI 1	coord: 13..274
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 24..58
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 24..53
None	No IPR available	PANTHER	PTHR31696:SF23	BNAC07G49860D PROTEIN	coord: 13..274

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G013600.1	Lsi06G013600.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010274	hydrotropism