Lsi06G012560 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G012560
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	tRNA pseudouridine synthase
Location	chr06: 23140294 .. 23147089 (-)
RNA-Seq Expression	Lsi06G012560
Synteny	Lsi06G012560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCCAAAACTACCGACAAGAGAGTCGAATTACTCAGGCCCCATTGGCTGGGCCTATTGGACTTCTCCAGTTGGACCAACATGGGCTTGCCCTCTTTCATTTCCTCGACTGCATCTTCTACGTGGATTTACAATCTCATCCCTGCTGCGACGGCGGCTGCAACTGCAAAACACGGTTGTCCGTTCTCAGGCAGGGCGGGGCGGTGGTTATGATACCAAATCCGAGGGCTAGACTTGTATCACCATCAGGTGCCGGTTTTGGTTTCAAGATTCTGATGGAGAATCGAGCTCAGCAAAAAGACTATCTTCACTATAATCACACTGATCGCTGCAGAAATGCTAGATGGACTGCCAGGTTTTTCTTTCGTACATTTTCATTATCTATCGCTTTATTTGATTTGAGATTTTACTCTTGGAATGAGGTTCGTACTTTGTTGTTGTAATTCAATCTGAATCTGAGAAGTCTGTCAATCAAGCTGTTATTATGCTATTGGCAGGGAAAGCTACCAACTCATGTATGGAAGGCCGTGGCAAGAAGTGCTCGATTTTTACTTTCATGCTGTTAATGGAAGGTCCTCACTAACCTCGTTGTTCGGAACTGAGGCGAGTCTCCAATTTCTACATTATCTTTAAACTTCGTTCCTCATTCTCTATAATTATTCCGTTTGTTGAAATGGAAGCAATTGAATCGTGAGCTACGACTCCTTTTCCCTCCCTTGCGAGACTATGAATCCTTCTCTCGTGTAATGCTAAGAGCAGTCTTTATATATCTTAAGCCTCTTTCGTTATAATGTTGTAGTTAAGAAATCCTTTTTTTTTTTTTTTTTCCCTCTGTTGAGGTTCTTTGATTCTTAAAATAATGGCTTCCATCTAAGAGAGCCAATTGCTTTAACATTGTACTGTGTAGGCTCTAAATTTAGCTTGGATTCTCAGTTCTGGAGATGATTTTTAATCAAATCCTTGCTATCAATTGTTACATTTGATTTATTGATCACGTTCATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATATTGTTTTATAAATTCCACTGGTTGATTAAAATTAGGAGAAATGGGGATGAAATTTTAGTTCTACAATTATCTTGTACAAAACAAGGAGATATCATTGATTTTTTTAATTTATTGTATATAGTACTTTATACACAGATACGTCTTGATCATGATGATGCTGAGACTCCATTGATTTACAACACAGCAGAGTTAAACAGAGCTTCAGTTAAAGATAAGACTGGGAGGTGGGCAAGAGCAACATTTAAGATTGTTCTTTCCTACCATGGAAATTCCTTTGATGGGTGGCAAAAGCAGCCAGGCTTGAATACTGTTCAGGGGTAAGCTTATTTCATGGAAGTTACCATATGCACAAGTAATATTGTATGCAATTGGGAGCTAGTGACGTTCTTTTCATTATCAGATCTATTAAACTGAAGAATTGTTGAAAACTTGTAATTTATAGTAAGCGTTTGAGAAGTTAGTTCTAGAGATACAATTGAAAATCTCATGCCTTCATTTCAAGTGAAACTTTCGTCTTGTGGACAATGTCCAAATTATGTCAATCAACCTTGTTAATCTCACCTTTCTGGTACTGTATCTTCTTCTTCCAGTATAATAGTTTGAAGTAATGTTTTAATATCTTTAGATGTATAAATAGTTTGATTTCAATCTAGAGATGGAAAACGCTGCTGTATATTGGAGGATTATGTCTACTTGTATTCTTTGGCAGCTTGGTAGAAAGATCTCTAGGGAAGTTTGTAGATGAGAAGAAAGCTCAGATGCTGAAAGATAGATGCTTACCAATAGAAGGATGTGCTGTGGTTGCTGGGCGCACTGACAAAGGGGTGACAGCTCTGCGACAAGTTTGCTCATTCTGTATGTTGGCTTTCTAATTTCTGTTGACGTGATTTTTTGATTGGATATTTAATTTCACAACTCTGCTTTCATTTTCTTAGATTCTGCTTTCATTTTCTTAGATTCTATCTCAGCTCAAATATGAACTCTTATATTAGAAATAGAATAAATTATATCTTTTATATTATTTCTTGACATCTTTGATAGTTGCAATGAATAGCCAAGCCATTAATTGCTTGTCACATCTGTTTCATGAACTCCACTTTTATTGTGCTTCACTTAAACACACAAATAGTCAAATAGGTGACTTTGTCTCGACTGCCCTATGCTCAAAGTGTTGGAAGGGTATGCCAAAAGACCTGTTAAAAGCAATTAGTATTGAAACAAGGGTGGTGCGAAGTCTATATTTAATATTATCACAACTCTTTTATTGATTAAAGTCTCATGAACATTTTCCTTTTTAACCAAACAGTAGGAGGTACCACACTGGAACTAAACCCCGGAATGACCTCACAATAAAAAGTAGCAACAGTAAAAAGGATTTGTGCGAACATGTCTTTTTTGTGGATAAGACGCACTGTATTTCTTTTAATTAAGCTAAATCCTTAGAAGCTTTGACCAAGATACCCCTCTCAGATTATTAATTCAAGTAGTTCTGCAGCATGGAAGTTGGAGTGCATCTCGTTTATTACCATCTGCAGAATTCCTTATCTGACGCTATAGAAAGGAAAAGTTGGCGAAGAAGATTGAAGTTTTTATTTGAATTATTTCCAATCTGTGCCTTTATACCCATCTCAGATTATTAATTCAAGTAGTTCTGCAGCATGGAAGTTGGAGTGTATCTTGTTTATTACCATCTGCAGAATTCCTTATCTGACGCTATAGAAAGGAAAAGTTGGCGAAGAAGATTGATGTTTTTATTTGAATTATTTCCAATCTGTGCCTTTATACCAATAATAGAATTCAAGCTAGACATCCAAACTACTCGGCCATCACAGTACCAATAATAAAATTCAACCTGAACCATGCTTCCTTTGTTTGAGTTCTTTAACATAAATATATACATGAAACTTGTTTAGAAAGAGCAATTACTATATGTATAGTTTTTGAGTTTTTTCTGTTTCTTTCCATCTTTGGTTTCAAATTACCTTTGGAGGGAAACATCCTCTTTCTGACTTCACCCACTTAATTTTTATTATTGTTTTCTATTTTAGAGAATCAATATTCGACTTCGGTTAAACTCCCATACTTGTAGATACTTGGCGCACAGATGTTAAACCTCAAGACATTGAAGATTCCATCAATAGTGCAGCTCCTGGAAAACTCAGAACATTATCTGTTTCCCAGGTAAAATTAAGGTTATTGGAAATTGATGTGGTTCCCCCGTGTCTAGCTTTAATAGTGTTGCAGATGTAACTTGTTTATTTCTCTTTCATACATGTAACTTCTCAAATTACTTACTGCTGTAGGTGTCTCGTATCTTCCATCCAAATTTCTCTGCTAAATGGAGGCGATATCTGTATATTTTCCCTCTGAATGATGGAGAGAACATGAAGCCAGATTATGCAATTTGGGAAGATGTAGAAAGCTGTAGAAAACAGGAAAATTATGATAAAATAGAAGTCTTAAATGTTGAAGAATGCAAGAACGAGATTCATGATAATTTGGTCCTTGGTCCAAATGATGAGGAGTTTGTGTCGGCTGATAAACCTCAAACATTTAGTATAAGGAGGGTTAACCAACTACTTAGGAAACTAGAAGGAAAGTTGTTGTCGTACAAGATGTTTGCACGTGACACCAAAGCATCAAGAAATGAGTGAGAATCATGCATTGATCCAATTATTCGCTTTATTTTTGTTAGTTATTCTAGCTTCTAATTTTAGTATCATAATTTTTTTTGTTTTTGTTTTGATTATATTTATCACTTTCTGCTCGGTAAGACCCTTGACATTTTTCTTATTCTGCTTATAAAGAAAATCGCTAATTTTCTTCTTATTCTGTTGGTCATTCTTTTAAACTCTTTTAGGAGCTATATATAGGAATGTGAAGAGGGGTGAATCCAGGCTTTGTGCTCTTTTGAGGGAGAGAGGGAGGGAGGGGATTAATGTGCTTGTATCCTGAACATGATCAGGATAGTTAGTCTATAGACTGACTAAACAAAGTTCTAGATTTTATCAGTCAAAAAGTTCTTTTTTAAAAATTTTGTCAATATGAGATTAATGTGCTTGCTTGTAGCTTTCTTTTTCCCAAACGTTTCCTAATCAATGACAGGGAGATGTTCTCGACTTTATGACATGTACTAATGGCAACTATCGTCCTTTTCAGAGGTCCTCCTACGGAGTGTTTTGTTTACCATGCACGAGCAGCAGAAGCTAGATTACCATGCTTGGTATTTTTCCTACTTCTGGTCCTCTTCATGCTCCATCCTCCTTTTCCTTCTACACTTCTCCCCTCTTGAAATCTATTTGTGGTGTTGTAACTGTGAAACAATATTCTATGCTCTAGCTCAGTAACCCTGAAAAGAAGTAAAAACATGTTATTTGCATTGTGTTTTTCTCTGATTCACATAGAAAACAGAAGATGAATTTGTGGAAGTGTATGAACCGAGGAAACATAGAAGAAATTGACTTTGACATTATATCTTCAACGTGGAACATAGCAAACTAGCTGTAGTATGTGCTCGATTCAATGAATATTCAAATTTGTTGCCCTTGCTCTCTCTTCTGTTTCTCTTCTTATTTGTTTTTGTATTCTAATCTTCTCTCTCTCTCTCTCTCTCTCCTTAGGAGAGGTTGTTGTCTCATGTTTATGTAGAGCTTTGTTTCTTAAATCGATACCTTATTCTTTGTCCTTTTTTGCTTGATTTTGCTTTATAGTGAAACTGTGAAAGCATGTTTGTTTCCTATTCGTGTCAACATGCTATCAACATTTTTCTCTATTGAACTGAACTGACTTCTTTCTGTCCAAATTTTTTTAGTTGTTTATCCAACAAAGTTAGTGATGTTAGTTAATGCAGCTACTCCAAATTTGGGGATCTGACTAAGTGGTGTTGTTCTATTGACAGGACCAAGGAGTAGGAAGAAGTGTTATGTGCGTTGAGCTGGTTGCTAATCGCTTCTTGCGGAAGGTATCTTATTCATCTCCGTGGTTGCCTATGCTATAAAGACCATATTGTACTGAACGTAAAATTGGTCTATTTAGATTTAGATTTAGATAATCCTTTCTTCAACTGCTTAAACTCGAGAGTTAACATCTTAAACGTCAGCAAGGGAATTTCCCCTTCGTTTGAGCAAAACGTGATACAACCATTAGGGCCATGTCCAAATGAAAAAGACACTAATTAATATGTCTTCTGTTGAATCATTGTTTCTTTAGCAGTTCAATCAATTCTAATGGAAGTTCAATCAGTTCTAATGGAATCTAATCTTAATATCCTACGAATTTCCTTAGCACCCAAATGTTGCTAGGTCAAACGAGTTGTCTCATAAAATTAGTCTAATGTGATATTAGTAATAAGCTGGCTCGGAGGCTCACGGATATTAATAATAATGATGATAAAATCTAATGTCACCCTTGCAGATGGTACGGGTGCTTGTTGCGACAGCAATTAGAGAGGCAGCTGCTGGTGCTGGAGAGGATGCGTTACTAGAGCTGATGGACGCCACATGTCGACGTGCCACTGCTCCACCTGCGCCTCCTGATGGCCTCAGTCTTGTTGATGTAGGATATACAGAATTTGATTCACGCAATTGCTTCATTTTAAATGACTAATTTTGTCCTCCTGATCAAAGTTGCTTAATAGGCTAATTGCCGCTTGGGGTATTATCCAACCTTTTGATTGGTGGTGTTATTATTACCATATTGATTTAGTGATTGAATTGTGTTAAGAGAAATATCTGCTTAGGGTTTCAAATTTGGCATGCAGTTTATCACACATCACACTTAGGGTGTTTGTATTTAAGGTTGGAGACGGTTTTGTTTGGATCAACTCTTCAAAAGGGTAGGATCAACCACTTTAGAGAGAATAAAT

mRNA sequence

GCCCAAAACTACCGACAAGAGAGTCGAATTACTCAGGCCCCATTGGCTGGGCCTATTGGACTTCTCCAGTTGGACCAACATGGGCTTGCCCTCTTTCATTTCCTCGACTGCATCTTCTACGTGGATTTACAATCTCATCCCTGCTGCGACGGCGGCTGCAACTGCAAAACACGGTTGTCCGTTCTCAGGCAGGGCGGGGCGGTGGTTATGATACCAAATCCGAGGGCTAGACTTGTATCACCATCAGGTGCCGGTTTTGGTTTCAAGATTCTGATGGAGAATCGAGCTCAGCAAAAAGACTATCTTCACTATAATCACACTGATCGCTGCAGAAATGCTAGATGGACTGCCAGGGAAAGCTACCAACTCATGTATGGAAGGCCGTGGCAAGAAGTGCTCGATTTTTACTTTCATGCTGTTAATGGAAGGTCCTCACTAACCTCGTTGTTCGGAACTGAGGCGATACTTTATACACAGATACGTCTTGATCATGATGATGCTGAGACTCCATTGATTTACAACACAGCAGAGTTAAACAGAGCTTCAGTTAAAGATAAGACTGGGAGGTGGGCAAGAGCAACATTTAAGATTGTTCTTTCCTACCATGGAAATTCCTTTGATGGGTGGCAAAAGCAGCCAGGCTTGAATACTGTTCAGGGCTTGGTAGAAAGATCTCTAGGGAAGTTTGTAGATGAGAAGAAAGCTCAGATGCTGAAAGATAGATGCTTACCAATAGAAGGATGTGCTGTGGTTGCTGGGCGCACTGACAAAGGGGTGACAGCTCTGCGACAAAATTCCTTATCTGACGCTATAGAAAGGAAAAGTTGGCGAAGAAGATTGATAGAATCAATATTCGACTTCGATACTTGGCGCACAGATGTTAAACCTCAAGACATTGAAGATTCCATCAATAGTGCAGCTCCTGGAAAACTCAGAACATTATCTGTTTCCCAGGTGTCTCGTATCTTCCATCCAAATTTCTCTGCTAAATGGAGGCGATATCTGTATATTTTCCCTCTGAATGATGGAGAGAACATGAAGCCAGATTATGCAATTTGGGAAGATGTAGAAAGCTGTAGAAAACAGGAAAATTATGATAAAATAGAAGTCTTAAATGTTGAAGAATGCAAGAACGAGATTCATGATAATTTGGTCCTTGGTCCAAATGATGAGGAGTTTGTGTCGGCTGATAAACCTCAAACATTTAGTATAAGGAGGGTTAACCAACTACTTAGGAAACTAGAAGGAAAGTTGTTGTCGTACAAGATGTTTGCACGTGACACCAAAGCATCAAGAAATGAGAATGTGAAGAGGGGTGAATCCAGGCTTTGTGCTCTTTTGAGGGAGAGAGGGAGGGAGGGGATTAATGACCAAGGAGTAGGAAGAAGTGTTATGTGCGTTGAGCTGGTTGCTAATCGCTTCTTGCGGAAGATGGTACGGGTGCTTGTTGCGACAGCAATTAGAGAGGCAGCTGCTGGTGCTGGAGAGGATGCGTTACTAGAGCTGATGGACGCCACATGTCGACGTGCCACTGCTCCACCTGCGCCTCCTGATGGCCTCAGTCTTGTTGATGTAGGATATACAGAATTTGATTCACGCAATTGCTTCATTTTAAATGACTAATTTTGTCCTCCTGATCAAAGTTGCTTAATAGGCTAATTGCCGCTTGGGGTATTATCCAACCTTTTGATTGGTGGTGTTATTATTACCATATTGATTTAGTGATTGAATTGTGTTAAGAGAAATATCTGCTTAGGGTTTCAAATTTGGCATGCAGTTTATCACACATCACACTTAGGGTGTTTGTATTTAAGGTTGGAGACGGTTTTGTTTGGATCAACTCTTCAAAAGGGTAGGATCAACCACTTTAGAGAGAATAAAT

Coding sequence (CDS)

GCCCAAAACTACCGACAAGAGAGTCGAATTACTCAGGCCCCATTGGCTGGGCCTATTGGACTTCTCCAGTTGGACCAACATGGGCTTGCCCTCTTTCATTTCCTCGACTGCATCTTCTACGTGGATTTACAATCTCATCCCTGCTGCGACGGCGGCTGCAACTGCAAAACACGGTTGTCCGTTCTCAGGCAGGGCGGGGCGGTGGTTATGATACCAAATCCGAGGGCTAGACTTGTATCACCATCAGGTGCCGGTTTTGGTTTCAAGATTCTGATGGAGAATCGAGCTCAGCAAAAAGACTATCTTCACTATAATCACACTGATCGCTGCAGAAATGCTAGATGGACTGCCAGGGAAAGCTACCAACTCATGTATGGAAGGCCGTGGCAAGAAGTGCTCGATTTTTACTTTCATGCTGTTAATGGAAGGTCCTCACTAACCTCGTTGTTCGGAACTGAGGCGATACTTTATACACAGATACGTCTTGATCATGATGATGCTGAGACTCCATTGATTTACAACACAGCAGAGTTAAACAGAGCTTCAGTTAAAGATAAGACTGGGAGGTGGGCAAGAGCAACATTTAAGATTGTTCTTTCCTACCATGGAAATTCCTTTGATGGGTGGCAAAAGCAGCCAGGCTTGAATACTGTTCAGGGCTTGGTAGAAAGATCTCTAGGGAAGTTTGTAGATGAGAAGAAAGCTCAGATGCTGAAAGATAGATGCTTACCAATAGAAGGATGTGCTGTGGTTGCTGGGCGCACTGACAAAGGGGTGACAGCTCTGCGACAAAATTCCTTATCTGACGCTATAGAAAGGAAAAGTTGGCGAAGAAGATTGATAGAATCAATATTCGACTTCGATACTTGGCGCACAGATGTTAAACCTCAAGACATTGAAGATTCCATCAATAGTGCAGCTCCTGGAAAACTCAGAACATTATCTGTTTCCCAGGTGTCTCGTATCTTCCATCCAAATTTCTCTGCTAAATGGAGGCGATATCTGTATATTTTCCCTCTGAATGATGGAGAGAACATGAAGCCAGATTATGCAATTTGGGAAGATGTAGAAAGCTGTAGAAAACAGGAAAATTATGATAAAATAGAAGTCTTAAATGTTGAAGAATGCAAGAACGAGATTCATGATAATTTGGTCCTTGGTCCAAATGATGAGGAGTTTGTGTCGGCTGATAAACCTCAAACATTTAGTATAAGGAGGGTTAACCAACTACTTAGGAAACTAGAAGGAAAGTTGTTGTCGTACAAGATGTTTGCACGTGACACCAAAGCATCAAGAAATGAGAATGTGAAGAGGGGTGAATCCAGGCTTTGTGCTCTTTTGAGGGAGAGAGGGAGGGAGGGGATTAATGACCAAGGAGTAGGAAGAAGTGTTATGTGCGTTGAGCTGGTTGCTAATCGCTTCTTGCGGAAGATGGTACGGGTGCTTGTTGCGACAGCAATTAGAGAGGCAGCTGCTGGTGCTGGAGAGGATGCGTTACTAGAGCTGATGGACGCCACATGTCGACGTGCCACTGCTCCACCTGCGCCTCCTGATGGCCTCAGTCTTGTTGATGTAGGATATACAGAATTTGATTCACGCAATTGCTTCATTTTAAATGACTAA

Protein sequence

AQNYRQESRITQAPLAGPIGLLQLDQHGLALFHFLDCIFYVDLQSHPCCDGGCNCKTRLSVLRQGGAVVMIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQEVLDFYFHAVNGRSSLTSLFGTEAILYTQIRLDHDDAETPLIYNTAELNRASVKDKTGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRTDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDGENMKPDYAIWEDVESCRKQENYDKIEVLNVEECKNEIHDNLVLGPNDEEFVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNENVKRGESRLCALLRERGREGINDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTEFDSRNCFILND

Homology

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BLAST of Lsi06G012560 vs. ExPASy Swiss-Prot
Match: B5YDX5 (tRNA pseudouridine synthase A OS=Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) OX=309799 GN=truA PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 5.7e-10
Identity = 51/160 (31.87%), Postives = 73/160 (45.62%), Query Frame = 0


Query: 195 FKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCAVVAGR 254
           +K+ LSY G  F G+QKQPG  TVQG +E+ L         ++L D  + + G    AGR
Sbjct: 4   WKVELSYIGKDFWGFQKQPGKRTVQGELEKVL---------KLLFDEDIKVIG----AGR 63

Query: 255 TDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPGKLRTL 314
           TD GV AL Q       E K++    +  +                  +N   PG ++  
Sbjct: 64  TDAGVHALGQVVNFKTKESKNFSCDKLYKV------------------LNKLLPGDIKIK 123

Query: 315 SVSQVSRIFHPNFSAKWRRYLYIFPLNDGENM-KPDYAIW 354
            V  V   FH  +SAK R Y+Y+   N+ +N+   DY  W
Sbjct: 124 KVEIVDDNFHARYSAKRRWYIYVIYNNEEKNLFLRDYCWW 132

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BLAST of Lsi06G012560 vs. ExPASy Swiss-Prot
Match: B3WAI6 (tRNA pseudouridine synthase A OS=Lactobacillus casei (strain BL23) OX=543734 GN=truA PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 4.8e-09
Identity = 43/142 (30.28%), Postives = 69/142 (48.59%), Query Frame = 0


Query: 195 FKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCAVVAGR 254
           +K+ L+Y G +F G+Q QP   TVQG+++++L K        M K + + ++G    +GR
Sbjct: 4   YKVTLAYDGTNFAGYQVQPKQRTVQGVLQKALTK--------MTKGQPVHVDG----SGR 63

Query: 255 TDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPGKLRTL 314
           TD GV AL Q                   +  FD +  ++  + +  ++NS  P  +  L
Sbjct: 64  TDSGVHALGQ-------------------VISFD-YPGNIPAESMLKAMNSLMPLDIEIL 113

Query: 315 SVSQVSRIFHPNFSAKWRRYLY 337
               V   FH  +SAK +RYLY
Sbjct: 124 KAEIVDADFHARYSAKGKRYLY 113

[Close]

BLAST of Lsi06G012560 vs. ExPASy Swiss-Prot
Match: Q035B5 (tRNA pseudouridine synthase A OS=Lactobacillus paracasei (strain ATCC 334 / BCRC 17002 / CIP 107868 / KCTC 3260 / NRRL B-441) OX=321967 GN=truA PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 4.8e-09
Identity = 43/142 (30.28%), Postives = 69/142 (48.59%), Query Frame = 0


Query: 195 FKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCAVVAGR 254
           +K+ L+Y G +F G+Q QP   TVQG+++++L K        M K + + ++G    +GR
Sbjct: 4   YKVTLAYDGTNFAGYQVQPKQRTVQGVLQKALTK--------MTKGQPVHVDG----SGR 63

Query: 255 TDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPGKLRTL 314
           TD GV AL Q                   +  FD +  ++  + +  ++NS  P  +  L
Sbjct: 64  TDSGVHALGQ-------------------VISFD-YPGNIPAESMLKAMNSLMPLDIEIL 113

Query: 315 SVSQVSRIFHPNFSAKWRRYLY 337
               V   FH  +SAK +RYLY
Sbjct: 124 KAEIVDADFHARYSAKGKRYLY 113

[Close]

BLAST of Lsi06G012560 vs. ExPASy Swiss-Prot
Match: A8F526 (tRNA pseudouridine synthase A OS=Pseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO) OX=416591 GN=truA PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 8.2e-09
Identity = 47/160 (29.38%), Postives = 75/160 (46.88%), Query Frame = 0


Query: 195 FKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCAVVAGR 254
           F  ++SY G +F G+Q Q  + TVQG+ E++L         ++LK R +      + AGR
Sbjct: 4   FMAIVSYDGTNFCGFQVQKDVRTVQGMFEQAL--------ERILKQRVI-----TIAAGR 63

Query: 255 TDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPGKLRTL 314
           TD GV A  Q                   I  FD +  D+  + +++++N+  P  +   
Sbjct: 64  TDTGVHANGQ-------------------IVCFDCY-LDIDEESMKNAMNANLPDDIYVR 123

Query: 315 SVSQVSRIFHPNFSAKWRRYLYIFPLNDGENM-KPDYAIW 354
            V +V + FHP F AK R Y Y+   +   N+   +YA W
Sbjct: 124 KVVEVDKNFHPRFDAKRRIYHYLIYNSQEPNLFIRNYAWW 130

[Close]

BLAST of Lsi06G012560 vs. ExPASy Swiss-Prot
Match: B0KCN3 (tRNA pseudouridine synthase A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=truA PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.1e-08
Identity = 78/332 (23.49%), Postives = 123/332 (37.05%), Query Frame = 0


Query: 197 IVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRTD 256
           IV+ Y G ++ GWQ Q    TVQ ++++++ K   E             E   + A RTD
Sbjct: 6   IVVEYDGTNYHGWQYQKNAVTVQEVLQKAIKKVTGE-------------EVNLIGASRTD 65

Query: 257 KGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPGKLRTLSV 316
            GV AL Q                   + +F T  T +  + +  ++NS  P  +  +  
Sbjct: 66  TGVHALYQ-------------------VANFKT-NTKIPAEKLPYALNSVLPDDIVVVQA 125

Query: 317 SQVSRIFHPNFSAKWRRYLYIFPLNDGENMKPDYAIWEDVESCRKQENYDKIEVLNVEEC 376
             V   FH  +SAK +RY YI  LN    M              +   +     LN+EE 
Sbjct: 126 KDVEDSFHARYSAKRKRYKYII-LNRKFQM-----------PTMRNYCWHISYPLNIEEM 185

Query: 377 KNEIHDNLVLGPND-EEFVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEN 436
           K     + ++G +D   F ++   +T +IR V                            
Sbjct: 186 KKAA--SYLIGTHDFSAFKASGSSKTSTIRTVYD-------------------------- 244

Query: 437 VKRGESRLCALLRERGREGINDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAG 496
                     L  E+  + IN          +E+ AN FL  MVR++V T          
Sbjct: 246 ----------LTIEKNEDFIN----------IEIEANGFLYNMVRIIVGTLSYVGLGKIK 244

Query: 497 EDALLELMDATCRRATAPPAPPDGLSLVDVGY 528
           ED + +++ +  R      APP GL L+ + Y
Sbjct: 306 EDEVYDILKSKDRTKAGITAPPQGLYLIKIIY 244

[Close]

BLAST of Lsi06G012560 vs. ExPASy TrEMBL
Match: A0A5A7UXE7 (tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004790 PE=3 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 5.9e-212
Identity = 387/474 (81.65%), Postives = 404/474 (85.23%), Query Frame = 0


Query: 70  MIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 129
           MIPNPRARLVSPS AGFG KILMENRAQQKDYLHYNHTDRCRNARWTARES+Q MYGRPW
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQQKDYLHYNHTDRCRNARWTARESFQFMYGRPW 60

Query: 130 QEVLDFYFHAVNGRSSLTSLFGTEAILYTQIRLDHDDAETPLIYNTAELNRASVKDKTGR 189
           QEVLDFY HAVNGR SL+SL GTE      I  DHDDAETPLIYN+AELNRA+VKDKTGR
Sbjct: 61  QEVLDFYSHAVNGRLSLSSLLGTE------ICRDHDDAETPLIYNSAELNRAAVKDKTGR 120

Query: 190 WARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCA 249
           WARA FKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKD+CLP+EGCA
Sbjct: 121 WARANFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDKCLPLEGCA 180

Query: 250 VVAGRTDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPG 309
           VVAGRTDKGVTALRQ                   +  F TWRTDVKPQDIEDSINSAAPG
Sbjct: 181 VVAGRTDKGVTALRQ-------------------VCSFYTWRTDVKPQDIEDSINSAAPG 240

Query: 310 KLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDGENMKPDYAIWEDVESCRKQENYDKIE 369
           KLRTLSVSQVSRIFHPNFSAKWRRYLYI PLNDGENM+PD AIWEDVE+ R++EN  KIE
Sbjct: 241 KLRTLSVSQVSRIFHPNFSAKWRRYLYILPLNDGENMEPDSAIWEDVEN-REEENCVKIE 300

Query: 370 VLNVEECKNEIHDNLVLGPNDEEFVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK 429
            LN E+C + IHDNLVL PND+E VSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK
Sbjct: 301 ALNGEKCVDGIHDNLVLSPNDDELVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK 360

Query: 430 ASRNENVKRGESRLCALLRERGRE---GINDQGVGRSVMCVELVANRFLRKMVRVLVATA 489
           ASRNE    G    C +   R  E      +QGVGRSVMCVELVANRFLRKMVRVLVATA
Sbjct: 361 ASRNE----GPPTECFVYHARAAETRLPCLEQGVGRSVMCVELVANRFLRKMVRVLVATA 420

Query: 490 IREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTEFDSRNCFILND 541
           IREAAAGAGEDALLELM ATCRRATAPPAPPDGLSLVDVGYTEFDS+ CFILND
Sbjct: 421 IREAAAGAGEDALLELMVATCRRATAPPAPPDGLSLVDVGYTEFDSQICFILND 444

[Close]

BLAST of Lsi06G012560 vs. ExPASy TrEMBL
Match: A0A1S3BWI6 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103494203 PE=3 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 5.9e-212
Identity = 387/474 (81.65%), Postives = 404/474 (85.23%), Query Frame = 0


Query: 70  MIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 129
           MIPNPRARLVSPS AGFG KILMENRAQQKDYLHYNHTDRCRNARWTARES+Q MYGRPW
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQQKDYLHYNHTDRCRNARWTARESFQFMYGRPW 60

Query: 130 QEVLDFYFHAVNGRSSLTSLFGTEAILYTQIRLDHDDAETPLIYNTAELNRASVKDKTGR 189
           QEVLDFY HAVNGR SL+SL GTE      I  DHDDAETPLIYN+AELNRA+VKDKTGR
Sbjct: 61  QEVLDFYSHAVNGRLSLSSLLGTE------ICRDHDDAETPLIYNSAELNRAAVKDKTGR 120

Query: 190 WARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCA 249
           WARA FKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKD+CLP+EGCA
Sbjct: 121 WARANFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDKCLPLEGCA 180

Query: 250 VVAGRTDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPG 309
           VVAGRTDKGVTALRQ                   +  F TWRTDVKPQDIEDSINSAAPG
Sbjct: 181 VVAGRTDKGVTALRQ-------------------VCSFYTWRTDVKPQDIEDSINSAAPG 240

Query: 310 KLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDGENMKPDYAIWEDVESCRKQENYDKIE 369
           KLRTLSVSQVSRIFHPNFSAKWRRYLYI PLNDGENM+PD AIWEDVE+ R++EN  KIE
Sbjct: 241 KLRTLSVSQVSRIFHPNFSAKWRRYLYILPLNDGENMEPDSAIWEDVEN-REEENCVKIE 300

Query: 370 VLNVEECKNEIHDNLVLGPNDEEFVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK 429
            LN E+C + IHDNLVL PND+E VSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK
Sbjct: 301 ALNGEKCVDGIHDNLVLSPNDDELVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK 360

Query: 430 ASRNENVKRGESRLCALLRERGRE---GINDQGVGRSVMCVELVANRFLRKMVRVLVATA 489
           ASRNE    G    C +   R  E      +QGVGRSVMCVELVANRFLRKMVRVLVATA
Sbjct: 361 ASRNE----GPPTECFVYHARAAETRLPCLEQGVGRSVMCVELVANRFLRKMVRVLVATA 420

Query: 490 IREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTEFDSRNCFILND 541
           IREAAAGAGEDALLELM ATCRRATAPPAPPDGLSLVDVGYTEFDS+ CFILND
Sbjct: 421 IREAAAGAGEDALLELMVATCRRATAPPAPPDGLSLVDVGYTEFDSQICFILND 444

[Close]

BLAST of Lsi06G012560 vs. ExPASy TrEMBL
Match: A0A0A0LV59 (tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G025830 PE=3 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 4.0e-208
Identity = 379/474 (79.96%), Postives = 401/474 (84.60%), Query Frame = 0


Query: 70  MIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 129
           MIPNPRARLVSPS AGFG KILMENRAQ+KDYLHYNHTDRCR+ARWTARESYQ MYGRPW
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQRKDYLHYNHTDRCRSARWTARESYQFMYGRPW 60

Query: 130 QEVLDFYFHAVNGRSSLTSLFGTEAILYTQIRLDHDDAETPLIYNTAELNRASVKDKTGR 189
           QEVLDFY HAVNGR SL+SL GTE      I  DHDDAETPLIYN+AELNRA+VKDKTGR
Sbjct: 61  QEVLDFYSHAVNGRFSLSSLLGTE------ICRDHDDAETPLIYNSAELNRAAVKDKTGR 120

Query: 190 WARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCA 249
           WARATFKIVLSY GNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKD+CLP+EGCA
Sbjct: 121 WARATFKIVLSYDGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDKCLPLEGCA 180

Query: 250 VVAGRTDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPG 309
           +VAGRTDKGVTALRQ                   +  F TWRTDVKPQDIEDSINSAAPG
Sbjct: 181 LVAGRTDKGVTALRQ-------------------VCSFYTWRTDVKPQDIEDSINSAAPG 240

Query: 310 KLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDGENMKPDYAIWEDVESCRKQENYDKIE 369
           KLRT SVSQVSRIFHPNFSAKWRRYLYI PLNDGENM+PD   WE+VE+ R++EN+ KIE
Sbjct: 241 KLRTSSVSQVSRIFHPNFSAKWRRYLYILPLNDGENMEPDSTTWENVEN-REEENFVKIE 300

Query: 370 VLNVEECKNEIHDNLVLGPNDEEFVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK 429
             N E+C + IHDNLVL PND+E VSADKPQTFSI RVNQLLRKLEGKLLSYKMFARDTK
Sbjct: 301 ASNGEKCVDGIHDNLVLSPNDQELVSADKPQTFSITRVNQLLRKLEGKLLSYKMFARDTK 360

Query: 430 ASRNENVKRGESRLCALLRERGRE---GINDQGVGRSVMCVELVANRFLRKMVRVLVATA 489
           ASRNE    G    C +   R  E      +QGVGRSVMCVELVANRFLRKMVRVLVATA
Sbjct: 361 ASRNE----GPPTECFVYHARAAETRLPCLEQGVGRSVMCVELVANRFLRKMVRVLVATA 420

Query: 490 IREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTEFDSRNCFILND 541
           IREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYT+FDS+ CFILND
Sbjct: 421 IREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTDFDSQICFILND 444

[Close]

BLAST of Lsi06G012560 vs. ExPASy TrEMBL
Match: A0A6J1C0W1 (tRNA pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111007251 PE=3 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 2.1e-201
Identity = 374/474 (78.90%), Postives = 393/474 (82.91%), Query Frame = 0


Query: 70  MIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 129
           MIPNPRARLV  S A FG K LMENRA QKDYLHYNHTDRCRNARWTARESYQLMYGRPW
Sbjct: 1   MIPNPRARLV--SSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 60

Query: 130 QEVLDFYFHAVNGRSSLTSLFGTEAILYTQIRLDHDDAETPLIYNTAELNRASVKDKTGR 189
           QEVLDFYFHAVNGR S +SLFGTE     +   DH+DAE PLIYNT+ELNRASVKDKTGR
Sbjct: 61  QEVLDFYFHAVNGRFSPSSLFGTE----VKTVHDHNDAEIPLIYNTSELNRASVKDKTGR 120

Query: 190 WARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCA 249
           WARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVE+SLGKFVDEKKAQ+LKDRCLP+EGCA
Sbjct: 121 WARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCA 180

Query: 250 VVAGRTDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPG 309
           VVAGRTDKGVTALRQ                   +  F TWRTDVKP DIEDSINSAAP 
Sbjct: 181 VVAGRTDKGVTALRQ-------------------VCSFYTWRTDVKPLDIEDSINSAAPR 240

Query: 310 KLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDGENMKPDYAIWEDVESCRKQENYDKIE 369
           KLR LSVSQVSR FHPNFSAKWRRYLYIFPL DGENM+PD+AI ED E+CR++ENY+KIE
Sbjct: 241 KLRVLSVSQVSRNFHPNFSAKWRRYLYIFPLKDGENMEPDFAIGEDAENCREEENYNKIE 300

Query: 370 VLNVEECKNEIHDNLVLGPNDEEFVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK 429
            LN  E K+EI DNLVL  N EE  S DKPQTFS+RRVN+LLRKLEGKLLSYKMFARDTK
Sbjct: 301 ALN-GEFKDEILDNLVLSQN-EELASDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTK 360

Query: 430 ASRNENVKRGESRLCALLRERGREG---INDQGVGRSVMCVELVANRFLRKMVRVLVATA 489
           ASRNE    G    C L   R  E      DQGVGRSVMCVELVANRFLRKMVRVLVATA
Sbjct: 361 ASRNE----GPPTECFLYHARAVEARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATA 420

Query: 490 IREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTEFDSRNCFILND 541
           IRE AAGAGEDALL LMDATCRRATAPPAPPDGLSLVDVGY+EFD +NCFILND
Sbjct: 421 IREVAAGAGEDALLRLMDATCRRATAPPAPPDGLSLVDVGYSEFDPQNCFILND 443

[Close]

BLAST of Lsi06G012560 vs. ExPASy TrEMBL
Match: A0A6J1L1W3 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499102 PE=3 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 1.2e-199
Identity = 372/488 (76.23%), Postives = 399/488 (81.76%), Query Frame = 0


Query: 62  LRQGGAVVMIPNPRARLVSPSGAGFGF------KILMENRAQQKDYLHYNHTDRCRNARW 121
           LR GGAVVM+PNPRARL+SPSGAGFGF      K LMENRAQ+KDYLHYNHTDRCRNARW
Sbjct: 35  LRLGGAVVMLPNPRARLLSPSGAGFGFGFGFGIKCLMENRAQKKDYLHYNHTDRCRNARW 94

Query: 122 TARESYQLMYGRPWQEVLDFYFHAVNGRSSLTSLFGTEAILYTQIRLDHDDAETPLIYNT 181
           T+RESYQLMY RPWQEVLDFY HAVNGR SL+S       L T+   DHDD E   + NT
Sbjct: 95  TSRESYQLMYERPWQEVLDFYSHAVNGRLSLSS-------LRTETVRDHDDTEIQPVCNT 154

Query: 182 AELNRASVKDKTGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKA 241
           AELNRA VKD TGRWARATFKIVLSYHGNSFDGWQKQP LNTVQG+VE+SLGKFVDEKKA
Sbjct: 155 AELNRAPVKDGTGRWARATFKIVLSYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKA 214

Query: 242 QMLKDRCLPIEGCAVVAGRTDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVK 301
           Q LKDRCLPIEGCAVVAGRTDKGVTALRQ                   +  F TWRTDVK
Sbjct: 215 QQLKDRCLPIEGCAVVAGRTDKGVTALRQ-------------------VCSFYTWRTDVK 274

Query: 302 PQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDGENMKPDYAIWED 361
           PQDIE SIN+AAPGKLR LSVSQVSR+FHPNFSAKWRRYLYIFPL DGENM+P++AI ED
Sbjct: 275 PQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRRYLYIFPLKDGENMEPNFAILED 334

Query: 362 VESCRKQENYDKIEVLNVEECKNEIHDNLVLGPNDEEFVSADKPQTFSIRRVNQLLRKLE 421
           VE+ R++E  DKIEVL+  ECK+EI +NL+L  +DEE  SA+KPQTFSI RVNQLLRKLE
Sbjct: 335 VENSREEEYRDKIEVLH-GECKDEILNNLILSQDDEEPASANKPQTFSIMRVNQLLRKLE 394

Query: 422 GKLLSYKMFARDTKASRNENVKRGESRLCALLRERGREG---INDQGVGRSVMCVELVAN 481
           GKLLSYKMFARDTKASRNE    G    C +   R  E      DQGVGRSVMCVELVAN
Sbjct: 395 GKLLSYKMFARDTKASRNE----GPPTECFVYHARAMEARLPCLDQGVGRSVMCVELVAN 454

Query: 482 RFLRKMVRVLVATAIREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTEFDS 541
           RFLRKMVRVLVATAIREAAAGAGEDALL+LMDATCRRATAPPAPPDGLSLVDVGYTEFD 
Sbjct: 455 RFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPPAPPDGLSLVDVGYTEFDP 491

[Close]

BLAST of Lsi06G012560 vs. NCBI nr
Match: XP_038878348.1 (tRNA pseudouridine synthase A [Benincasa hispida])

HSP 1 Score: 794.3 bits (2050), Expect = 6.7e-226
Identity = 404/474 (85.23%), Postives = 415/474 (87.55%), Query Frame = 0


Query: 70  MIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 129
           MIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPW
Sbjct: 1   MIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 60

Query: 130 QEVLDFYFHAVNGRSSLTSLFGTEAILYTQIRLDHDDAETPLIYNTAELNRASVKDKTGR 189
           QEVLDFYFHAVNGR SL+SLFGTE      I  DHDDAE PLIYNTAELNR SVKDKTGR
Sbjct: 61  QEVLDFYFHAVNGRFSLSSLFGTE------IGHDHDDAEIPLIYNTAELNRVSVKDKTGR 120

Query: 190 WARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCA 249
           WARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVE+SLGKFVD+KKAQMLKD+CLPIEGCA
Sbjct: 121 WARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDDKKAQMLKDKCLPIEGCA 180

Query: 250 VVAGRTDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPG 309
           VVAGRTDKGVTALRQ                   +  F TWRTDV+PQDIEDSIN AAPG
Sbjct: 181 VVAGRTDKGVTALRQ-------------------VCSFYTWRTDVQPQDIEDSINRAAPG 240

Query: 310 KLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDGENMKPDYAIWEDVESCRKQENYDKIE 369
           KLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDGENM+PDYAIWEDVE CRK+ENY KIE
Sbjct: 241 KLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDGENMEPDYAIWEDVEDCRKEENYGKIE 300

Query: 370 VLNVEECKNEIHDNLVLGPNDEEFVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK 429
           VLN EECK+EIHDNLV GPNDEE VSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK
Sbjct: 301 VLNGEECKDEIHDNLVFGPNDEELVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK 360

Query: 430 ASRNENVKRGESRLCALLRERGREG---INDQGVGRSVMCVELVANRFLRKMVRVLVATA 489
           ASRNE    G    C +   R  E      DQGVGRSVMCVELVANRFLRKMVRVLVATA
Sbjct: 361 ASRNE----GPPTECFVYHARAVEARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATA 420

Query: 490 IREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTEFDSRNCFILND 541
           IREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTEFDS+NCFILND
Sbjct: 421 IREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTEFDSQNCFILND 445

[Close]

BLAST of Lsi06G012560 vs. NCBI nr
Match: XP_008453520.1 (PREDICTED: tRNA pseudouridine synthase A [Cucumis melo] >KAA0058161.1 tRNA pseudouridine synthase A [Cucumis melo var. makuwa])

HSP 1 Score: 746.9 bits (1927), Expect = 1.2e-211
Identity = 387/474 (81.65%), Postives = 404/474 (85.23%), Query Frame = 0


Query: 70  MIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 129
           MIPNPRARLVSPS AGFG KILMENRAQQKDYLHYNHTDRCRNARWTARES+Q MYGRPW
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQQKDYLHYNHTDRCRNARWTARESFQFMYGRPW 60

Query: 130 QEVLDFYFHAVNGRSSLTSLFGTEAILYTQIRLDHDDAETPLIYNTAELNRASVKDKTGR 189
           QEVLDFY HAVNGR SL+SL GTE      I  DHDDAETPLIYN+AELNRA+VKDKTGR
Sbjct: 61  QEVLDFYSHAVNGRLSLSSLLGTE------ICRDHDDAETPLIYNSAELNRAAVKDKTGR 120

Query: 190 WARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCA 249
           WARA FKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKD+CLP+EGCA
Sbjct: 121 WARANFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDKCLPLEGCA 180

Query: 250 VVAGRTDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPG 309
           VVAGRTDKGVTALRQ                   +  F TWRTDVKPQDIEDSINSAAPG
Sbjct: 181 VVAGRTDKGVTALRQ-------------------VCSFYTWRTDVKPQDIEDSINSAAPG 240

Query: 310 KLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDGENMKPDYAIWEDVESCRKQENYDKIE 369
           KLRTLSVSQVSRIFHPNFSAKWRRYLYI PLNDGENM+PD AIWEDVE+ R++EN  KIE
Sbjct: 241 KLRTLSVSQVSRIFHPNFSAKWRRYLYILPLNDGENMEPDSAIWEDVEN-REEENCVKIE 300

Query: 370 VLNVEECKNEIHDNLVLGPNDEEFVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK 429
            LN E+C + IHDNLVL PND+E VSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK
Sbjct: 301 ALNGEKCVDGIHDNLVLSPNDDELVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK 360

Query: 430 ASRNENVKRGESRLCALLRERGRE---GINDQGVGRSVMCVELVANRFLRKMVRVLVATA 489
           ASRNE    G    C +   R  E      +QGVGRSVMCVELVANRFLRKMVRVLVATA
Sbjct: 361 ASRNE----GPPTECFVYHARAAETRLPCLEQGVGRSVMCVELVANRFLRKMVRVLVATA 420

Query: 490 IREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTEFDSRNCFILND 541
           IREAAAGAGEDALLELM ATCRRATAPPAPPDGLSLVDVGYTEFDS+ CFILND
Sbjct: 421 IREAAAGAGEDALLELMVATCRRATAPPAPPDGLSLVDVGYTEFDSQICFILND 444

[Close]

BLAST of Lsi06G012560 vs. NCBI nr
Match: XP_004137363.1 (uncharacterized protein LOC101205562 [Cucumis sativus] >KGN63901.1 hypothetical protein Csa_013558 [Cucumis sativus])

HSP 1 Score: 734.2 bits (1894), Expect = 8.2e-208
Identity = 379/474 (79.96%), Postives = 401/474 (84.60%), Query Frame = 0


Query: 70  MIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 129
           MIPNPRARLVSPS AGFG KILMENRAQ+KDYLHYNHTDRCR+ARWTARESYQ MYGRPW
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQRKDYLHYNHTDRCRSARWTARESYQFMYGRPW 60

Query: 130 QEVLDFYFHAVNGRSSLTSLFGTEAILYTQIRLDHDDAETPLIYNTAELNRASVKDKTGR 189
           QEVLDFY HAVNGR SL+SL GTE      I  DHDDAETPLIYN+AELNRA+VKDKTGR
Sbjct: 61  QEVLDFYSHAVNGRFSLSSLLGTE------ICRDHDDAETPLIYNSAELNRAAVKDKTGR 120

Query: 190 WARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCA 249
           WARATFKIVLSY GNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKD+CLP+EGCA
Sbjct: 121 WARATFKIVLSYDGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDKCLPLEGCA 180

Query: 250 VVAGRTDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPG 309
           +VAGRTDKGVTALRQ                   +  F TWRTDVKPQDIEDSINSAAPG
Sbjct: 181 LVAGRTDKGVTALRQ-------------------VCSFYTWRTDVKPQDIEDSINSAAPG 240

Query: 310 KLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDGENMKPDYAIWEDVESCRKQENYDKIE 369
           KLRT SVSQVSRIFHPNFSAKWRRYLYI PLNDGENM+PD   WE+VE+ R++EN+ KIE
Sbjct: 241 KLRTSSVSQVSRIFHPNFSAKWRRYLYILPLNDGENMEPDSTTWENVEN-REEENFVKIE 300

Query: 370 VLNVEECKNEIHDNLVLGPNDEEFVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK 429
             N E+C + IHDNLVL PND+E VSADKPQTFSI RVNQLLRKLEGKLLSYKMFARDTK
Sbjct: 301 ASNGEKCVDGIHDNLVLSPNDQELVSADKPQTFSITRVNQLLRKLEGKLLSYKMFARDTK 360

Query: 430 ASRNENVKRGESRLCALLRERGRE---GINDQGVGRSVMCVELVANRFLRKMVRVLVATA 489
           ASRNE    G    C +   R  E      +QGVGRSVMCVELVANRFLRKMVRVLVATA
Sbjct: 361 ASRNE----GPPTECFVYHARAAETRLPCLEQGVGRSVMCVELVANRFLRKMVRVLVATA 420

Query: 490 IREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTEFDSRNCFILND 541
           IREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYT+FDS+ CFILND
Sbjct: 421 IREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTDFDSQICFILND 444

[Close]

BLAST of Lsi06G012560 vs. NCBI nr
Match: XP_022135239.1 (uncharacterized protein LOC111007251 isoform X1 [Momordica charantia])

HSP 1 Score: 711.8 bits (1836), Expect = 4.4e-201
Identity = 374/474 (78.90%), Postives = 393/474 (82.91%), Query Frame = 0


Query: 70  MIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 129
           MIPNPRARLV  S A FG K LMENRA QKDYLHYNHTDRCRNARWTARESYQLMYGRPW
Sbjct: 1   MIPNPRARLV--SSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 60

Query: 130 QEVLDFYFHAVNGRSSLTSLFGTEAILYTQIRLDHDDAETPLIYNTAELNRASVKDKTGR 189
           QEVLDFYFHAVNGR S +SLFGTE     +   DH+DAE PLIYNT+ELNRASVKDKTGR
Sbjct: 61  QEVLDFYFHAVNGRFSPSSLFGTE----VKTVHDHNDAEIPLIYNTSELNRASVKDKTGR 120

Query: 190 WARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCA 249
           WARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVE+SLGKFVDEKKAQ+LKDRCLP+EGCA
Sbjct: 121 WARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCA 180

Query: 250 VVAGRTDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPG 309
           VVAGRTDKGVTALRQ                   +  F TWRTDVKP DIEDSINSAAP 
Sbjct: 181 VVAGRTDKGVTALRQ-------------------VCSFYTWRTDVKPLDIEDSINSAAPR 240

Query: 310 KLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDGENMKPDYAIWEDVESCRKQENYDKIE 369
           KLR LSVSQVSR FHPNFSAKWRRYLYIFPL DGENM+PD+AI ED E+CR++ENY+KIE
Sbjct: 241 KLRVLSVSQVSRNFHPNFSAKWRRYLYIFPLKDGENMEPDFAIGEDAENCREEENYNKIE 300

Query: 370 VLNVEECKNEIHDNLVLGPNDEEFVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTK 429
            LN  E K+EI DNLVL  N EE  S DKPQTFS+RRVN+LLRKLEGKLLSYKMFARDTK
Sbjct: 301 ALN-GEFKDEILDNLVLSQN-EELASDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTK 360

Query: 430 ASRNENVKRGESRLCALLRERGREG---INDQGVGRSVMCVELVANRFLRKMVRVLVATA 489
           ASRNE    G    C L   R  E      DQGVGRSVMCVELVANRFLRKMVRVLVATA
Sbjct: 361 ASRNE----GPPTECFLYHARAVEARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATA 420

Query: 490 IREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTEFDSRNCFILND 541
           IRE AAGAGEDALL LMDATCRRATAPPAPPDGLSLVDVGY+EFD +NCFILND
Sbjct: 421 IREVAAGAGEDALLRLMDATCRRATAPPAPPDGLSLVDVGYSEFDPQNCFILND 443

[Close]

BLAST of Lsi06G012560 vs. NCBI nr
Match: XP_023006334.1 (uncharacterized protein LOC111499102 isoform X1 [Cucurbita maxima])

HSP 1 Score: 706.1 bits (1821), Expect = 2.4e-199
Identity = 372/488 (76.23%), Postives = 399/488 (81.76%), Query Frame = 0


Query: 62  LRQGGAVVMIPNPRARLVSPSGAGFGF------KILMENRAQQKDYLHYNHTDRCRNARW 121
           LR GGAVVM+PNPRARL+SPSGAGFGF      K LMENRAQ+KDYLHYNHTDRCRNARW
Sbjct: 35  LRLGGAVVMLPNPRARLLSPSGAGFGFGFGFGIKCLMENRAQKKDYLHYNHTDRCRNARW 94

Query: 122 TARESYQLMYGRPWQEVLDFYFHAVNGRSSLTSLFGTEAILYTQIRLDHDDAETPLIYNT 181
           T+RESYQLMY RPWQEVLDFY HAVNGR SL+S       L T+   DHDD E   + NT
Sbjct: 95  TSRESYQLMYERPWQEVLDFYSHAVNGRLSLSS-------LRTETVRDHDDTEIQPVCNT 154

Query: 182 AELNRASVKDKTGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKA 241
           AELNRA VKD TGRWARATFKIVLSYHGNSFDGWQKQP LNTVQG+VE+SLGKFVDEKKA
Sbjct: 155 AELNRAPVKDGTGRWARATFKIVLSYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKA 214

Query: 242 QMLKDRCLPIEGCAVVAGRTDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVK 301
           Q LKDRCLPIEGCAVVAGRTDKGVTALRQ                   +  F TWRTDVK
Sbjct: 215 QQLKDRCLPIEGCAVVAGRTDKGVTALRQ-------------------VCSFYTWRTDVK 274

Query: 302 PQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDGENMKPDYAIWED 361
           PQDIE SIN+AAPGKLR LSVSQVSR+FHPNFSAKWRRYLYIFPL DGENM+P++AI ED
Sbjct: 275 PQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRRYLYIFPLKDGENMEPNFAILED 334

Query: 362 VESCRKQENYDKIEVLNVEECKNEIHDNLVLGPNDEEFVSADKPQTFSIRRVNQLLRKLE 421
           VE+ R++E  DKIEVL+  ECK+EI +NL+L  +DEE  SA+KPQTFSI RVNQLLRKLE
Sbjct: 335 VENSREEEYRDKIEVLH-GECKDEILNNLILSQDDEEPASANKPQTFSIMRVNQLLRKLE 394

Query: 422 GKLLSYKMFARDTKASRNENVKRGESRLCALLRERGREG---INDQGVGRSVMCVELVAN 481
           GKLLSYKMFARDTKASRNE    G    C +   R  E      DQGVGRSVMCVELVAN
Sbjct: 395 GKLLSYKMFARDTKASRNE----GPPTECFVYHARAMEARLPCLDQGVGRSVMCVELVAN 454

Query: 482 RFLRKMVRVLVATAIREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVGYTEFDS 541
           RFLRKMVRVLVATAIREAAAGAGEDALL+LMDATCRRATAPPAPPDGLSLVDVGYTEFD 
Sbjct: 455 RFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPPAPPDGLSLVDVGYTEFDP 491

[Close]

BLAST of Lsi06G012560 vs. TAIR 10
Match: AT5G35400.1 (Pseudouridine synthase family protein )

HSP 1 Score: 423.3 bits (1087), Expect = 2.9e-118
Identity = 235/444 (52.93%), Postives = 294/444 (66.22%), Query Frame = 0


Query: 101 YLHYNHTDRCRNARWTARESYQLMYGRPWQEVLDFYFHAVNGRSSLTSLFGTEAILYTQI 160
           Y+HY+H D C  ARW ARE+++ MY RPWQ VLDFY +AV G  S+ SLF  +  L    
Sbjct: 6   YVHYHHADACSKARWNARETFRFMYDRPWQHVLDFYSNAVEGNLSVLSLFQPKKSLV--- 65

Query: 161 RLDHDDAETPLIYNTAELNRASVKD-KTGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQ 220
              HDD  T  +   +E   ++ KD ++GRW R  FKIVLSY+G SFDGWQKQP L+TVQ
Sbjct: 66  ---HDDGITEEMLLESEPETSTRKDGRSGRWERVNFKIVLSYNGASFDGWQKQPDLHTVQ 125

Query: 221 GLVERSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRTDKGVTALRQNSLSDAIERKSWRRR 280
           G+VE+SLG FVDE+KAQ+LK +C P+EG  +VAGRTDKGV+AL Q               
Sbjct: 126 GVVEKSLGGFVDERKAQLLKKKCKPLEGRVLVAGRTDKGVSALNQ--------------- 185

Query: 281 LIESIFDFDTWRTDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRYLYIFP 340
               +  F TWR D++P  IED+IN  A GKLR +S+S+VSR FHPNFSAKWRRYLYIFP
Sbjct: 186 ----VCSFYTWRKDIEPIAIEDAINKDASGKLRVVSISKVSRSFHPNFSAKWRRYLYIFP 245

Query: 341 LNDGENMKPDYAIWEDV---ESCRKQENYDKIEVLNVEECKNEIHDNLVLGPNDEEFVSA 400
           L+D E ++      E+    E+ +KQ N    E +  E   +E  D L +          
Sbjct: 246 LDDAEPLRKSGENHENFIFDENKKKQRNGLLSEEIAEEVIMSE-DDELEIEETSNALEVV 305

Query: 401 DKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNENVKRGESRLCALLRERGRE--- 460
           +KP  FS+ +V+QLL+ L+GK+LSYKMFARDTKA+RNE    G    C +   R  E   
Sbjct: 306 EKPSDFSVSKVDQLLQHLQGKVLSYKMFARDTKAARNE----GPPTECFMYHARAAEIRL 365

Query: 461 GINDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLELMDATCRRATAP 520
             +D   GR VMCVELVANRFLRKMVRVLVAT+IREAAAGA +DALL+L++A+CRRATAP
Sbjct: 366 SSSDCVEGRRVMCVELVANRFLRKMVRVLVATSIREAAAGAEDDALLKLLEASCRRATAP 419

Query: 521 PAPPDGLSLVDVGYTEFDSRNCFI 538
           PAP +GL L DVGYT+FD +   I
Sbjct: 426 PAPSEGLCLFDVGYTDFDPQTSLI 419

[Close]

BLAST of Lsi06G012560 vs. TAIR 10
Match: AT5G35400.2 (Pseudouridine synthase family protein )

HSP 1 Score: 405.2 bits (1040), Expect = 8.1e-113
Identity = 237/491 (48.27%), Postives = 300/491 (61.10%), Query Frame = 0


Query: 101 YLHYNHTDRCRNARWTARESYQLMYGRPWQEVLDFYFHAVNGRSSLTSLFGTEAILYTQI 160
           Y+HY+H D C  ARW ARE+++ MY RPWQ VLDFY +AV G  S+ SLF  +  L    
Sbjct: 6   YVHYHHADACSKARWNARETFRFMYDRPWQHVLDFYSNAVEGNLSVLSLFQPKKSLV--- 65

Query: 161 RLDHDDAETPLIYNTAELNRASVKD-KTGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQ 220
              HDD  T  +   +E   ++ KD ++GRW R  FKIVLSY+G SFDGWQKQP L+TVQ
Sbjct: 66  ---HDDGITEEMLLESEPETSTRKDGRSGRWERVNFKIVLSYNGASFDGWQKQPDLHTVQ 125

Query: 221 GLVERSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRTDKGVTALRQNSLSDAIERKSWRRR 280
           G+VE+SLG FVDE+KAQ+LK +C P+EG  +VAGRTDKGV+AL Q               
Sbjct: 126 GVVEKSLGGFVDERKAQLLKKKCKPLEGRVLVAGRTDKGVSALNQ--------------- 185

Query: 281 LIESIFDFDTWRTDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRYLYIFP 340
               +  F TWR D++P  IED+IN  A GKLR +S+S+VSR FHPNFSAKWRRYLYIFP
Sbjct: 186 ----VCSFYTWRKDIEPIAIEDAINKDASGKLRVVSISKVSRSFHPNFSAKWRRYLYIFP 245

Query: 341 LNDGENMKPDYAIWEDV---ESCRKQENYDKIEVLNVEECKNEIHDNLVLGPNDEEFVSA 400
           L+D E ++      E+    E+ +KQ N    E +  E   +E  D L +          
Sbjct: 246 LDDAEPLRKSGENHENFIFDENKKKQRNGLLSEEIAEEVIMSE-DDELEIEETSNALEVV 305

Query: 401 DKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNE---------NVKRGESRLCA-- 460
           +KP  FS+ +V+QLL+ L+GK+LSYKMFARDTKA+RNE         + +  E RL +  
Sbjct: 306 EKPSDFSVSKVDQLLQHLQGKVLSYKMFARDTKAARNEGPPTECFMYHARAAEIRLSSSV 365

Query: 461 ------------------------------------LLRERGREGI---NDQGVGRSVMC 520
                                                L +R  E +   +D   GR VMC
Sbjct: 366 VHFVAWVFGGSIVLVILATEGSTLDLTDTKLYIASWFLIKRSCEFLFIASDCVEGRRVMC 425

Query: 521 VELVANRFLRKMVRVLVATAIREAAAGAGEDALLELMDATCRRATAPPAPPDGLSLVDVG 538
           VELVANRFLRKMVRVLVAT+IREAAAGA +DALL+L++A+CRRATAPPAP +GL L DVG
Sbjct: 426 VELVANRFLRKMVRVLVATSIREAAAGAEDDALLKLLEASCRRATAPPAPSEGLCLFDVG 470

[Close]

BLAST of Lsi06G012560 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 48.9 bits (115), Expect = 1.5e-05
Identity = 45/190 (23.68%), Postives = 73/190 (38.42%), Query Frame = 0


Query: 189 RWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSL---GKFVDEKKAQMLKDRCLPI 248
           ++ R    IV ++ G  + G QK PG  T++G +E +L   G   D        DR  P 
Sbjct: 54  KYRRRKVAIVFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPD-------ADRNKPR 113

Query: 249 EGCAVVAGRTDKGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINS 308
                 + RTDKGV+A+ Q                        + R  V P    + +NS
Sbjct: 114 NYEWARSARTDKGVSAVGQ----------------------VVSGRFYVDPPGFVERLNS 173

Query: 309 AAPGKLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDGENMKPDYAIWEDVESCRKQENY 368
             P ++R     +V+  F        RRY+Y+ P+        D  +  + E  R    Y
Sbjct: 174 KLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPV-----FALDPCVHGEAEMVRTDLGY 209

Query: 369 DKIEVLNVEE 376
           + ++ +   E
Sbjct: 234 EYVKCVECSE 209

[Close]

BLAST of Lsi06G012560 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 43.9 bits (102), Expect = 4.8e-04
Identity = 35/144 (24.31%), Postives = 57/144 (39.58%), Query Frame = 0


Query: 197 IVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRTD 256
           I+ ++ G  + G QK PG  T++G +E +L       ++   K +       A    RTD
Sbjct: 26  IIFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA----RTD 85

Query: 257 KGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPGKLRTLSV 316
           KGV+A+ Q                        + R  V P    + +NS  P ++R    
Sbjct: 86  KGVSAVGQ----------------------VVSGRFIVDPLGFVNRLNSNLPNQIRIFGY 143

Query: 317 SQVSRIFHPNFSAKWRRYLYIFPL 341
             V+  F        RRY+Y+ P+
Sbjct: 146 KHVTPSFSSKKFCDRRRYVYLLPV 143

[Close]

BLAST of Lsi06G012560 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 43.9 bits (102), Expect = 4.8e-04
Identity = 35/144 (24.31%), Postives = 57/144 (39.58%), Query Frame = 0


Query: 197 IVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRTD 256
           I+ ++ G  + G QK PG  T++G +E +L       ++   K +       A    RTD
Sbjct: 26  IIFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA----RTD 85

Query: 257 KGVTALRQNSLSDAIERKSWRRRLIESIFDFDTWRTDVKPQDIEDSINSAAPGKLRTLSV 316
           KGV+A+ Q                        + R  V P    + +NS  P ++R    
Sbjct: 86  KGVSAVGQ----------------------VVSGRFIVDPLGFVNRLNSNLPNQIRIFGY 143

Query: 317 SQVSRIFHPNFSAKWRRYLYIFPL 341
             V+  F        RRY+Y+ P+
Sbjct: 146 KHVTPSFSSKKFCDRRRYVYLLPV 143

The following BLAST results are available for this feature:

BLAST of Lsi06G012560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5YDX5	5.7e-10	31.88	tRNA pseudouridine synthase A OS=Dictyoglomus thermophilum (strain ATCC 35947 / ...	[more]
B3WAI6	4.8e-09	30.28	tRNA pseudouridine synthase A OS=Lactobacillus casei (strain BL23) OX=543734 GN=...	[more]
Q035B5	4.8e-09	30.28	tRNA pseudouridine synthase A OS=Lactobacillus paracasei (strain ATCC 334 / BCRC...	[more]
A8F526	8.2e-09	29.38	tRNA pseudouridine synthase A OS=Pseudothermotoga lettingae (strain ATCC BAA-301...	[more]
B0KCN3	1.1e-08	23.49	tRNA pseudouridine synthase A OS=Thermoanaerobacter pseudethanolicus (strain ATC...	[more]

BLAST of Lsi06G012560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UXE7	5.9e-212	81.65	tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A1S3BWI6	5.9e-212	81.65	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103494203 PE=3 SV=1	[more]
A0A0A0LV59	4.0e-208	79.96	tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G025830 PE=3 SV=1	[more]
A0A6J1C0W1	2.1e-201	78.90	tRNA pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111007251 PE=3 ...	[more]
A0A6J1L1W3	1.2e-199	76.23	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499102 PE=3 SV=...	[more]

BLAST of Lsi06G012560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878348.1	6.7e-226	85.23	tRNA pseudouridine synthase A [Benincasa hispida]	[more]
XP_008453520.1	1.2e-211	81.65	PREDICTED: tRNA pseudouridine synthase A [Cucumis melo] >KAA0058161.1 tRNA pseud...	[more]
XP_004137363.1	8.2e-208	79.96	uncharacterized protein LOC101205562 [Cucumis sativus] >KGN63901.1 hypothetical ...	[more]
XP_022135239.1	4.4e-201	78.90	uncharacterized protein LOC111007251 isoform X1 [Momordica charantia]	[more]
XP_023006334.1	2.4e-199	76.23	uncharacterized protein LOC111499102 isoform X1 [Cucurbita maxima]	[more]

BLAST of Lsi06G012560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35400.1	2.9e-118	52.93	Pseudouridine synthase family protein	[more]
AT5G35400.2	8.1e-113	48.27	Pseudouridine synthase family protein	[more]
AT1G20370.1	1.5e-05	23.68	Pseudouridine synthase family protein	[more]
AT1G76120.1	4.8e-04	24.31	Pseudouridine synthase family protein	[more]
AT1G76120.2	4.8e-04	24.31	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 330..529 e-value: 5.5E-14 score: 54.1
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 427..527 e-value: 1.3E-6 score: 28.8
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 192..329 e-value: 1.3E-18 score: 69.2
None	No IPR available	PIRSR	PIRSR001430-2	PIRSR001430-2	coord: 250..353 e-value: 2.6E-10 score: 38.2
None	No IPR available	PANTHER	PTHR11142:SF10	TRNA PSEUDOURIDINE SYNTHASE	coord: 108..537
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 108..537
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 194..533

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G012560.1	Lsi06G012560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031119	tRNA pseudouridine synthesis
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0106029	tRNA pseudouridine synthase activity
molecular_function	GO:0009982	pseudouridine synthase activity