Lsi06G012160 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi06G012160 vs. ExPASy Swiss-Prot
Match: E9Q5C9 (Nucleolar and coiled-body phosphoprotein 1 OS=Mus musculus OX=10090 GN=Nolc1 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 6.4e-07
Identity = 96/366 (26.23%), Postives = 142/366 (38.80%), Query Frame = 0

Query: 98  KHLKKCSQAITTQDKPDEEMVKEVADDRVPEAEEEPTKKCKDKKKTKKNAVPEMHADAAE 157
           K  +  S +  +    DE   K V+  + P+    P          K    P+  A + +
Sbjct: 366 KKAESSSDSSDSDSSEDEAPAKPVSTTKSPKPAVTPKPSA-----AKAVTTPKQPAGSNQ 425

Query: 158 KEKSEPVHVESLNNSNGTTVYEEAGKKSKDKKKKKNKEKLETVATISDDVAVDSIGLNSD 217
           K +S      S    + ++  EEA KKS    K K   K              S   +S 
Sbjct: 426 KPQSRKADSSSSEEESSSSEEEEASKKSATTPKAKVTAKAAPAKQAPQAAGDSSSDSDSS 485

Query: 218 VTTIEEKVVKSKTKKKKDGRHLSD-------ENSNQLNDGTNMLHEEEQNDNSKKRKRL- 277
            +  EEK  K   KKK  G  +S        E  +  +  ++   +  + +  KK K   
Sbjct: 486 SSEEEEKTPKPPAKKKAAGGAVSTPAPGKKAEAKSSSSSSSSSSEDSSEEEKKKKPKATT 545

Query: 278 ----ASEDN------NNHTVDEKETEDVKRRKLEGSKGCNDGVQSTKVDADAGNRSTINE 337
               AS+ N      N     E E E+ +    E  K              +G +   NE
Sbjct: 546 PKIQASKANGTPASLNGKAAKESEEEEEEEETEEKKKAAG-------TKPGSGKKRKQNE 605

Query: 338 LSPQTNECVEKTAEKTSTKKALKKHSNGSTEPKTINAFQRVKVDAVTFADEKLADNSYWA 397
            + +      K   K  T     K   G  E +  + F+RV+ + +   D ++ADNS+ A
Sbjct: 606 TADEATTPQAKKV-KLETPNTFPKRKKG--ERRASSPFRRVREEEIE-VDSRVADNSFDA 665

Query: 398 KDFLFLICNLSYYTEQGGADTGYGAKAQEVLGQVKGRDFRHEKTKKKRGSYRGGVIDLQS 446
           K                GA   +G +A +VL   KG+ FRHEKTKKKRGSYRGG I +Q 
Sbjct: 666 K---------------RGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQV 700

BLAST of Lsi06G012160 vs. ExPASy Swiss-Prot
Match: P32583 (Suppressor protein SRP40 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SRP40 PE=1 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 1.0e-04
Identity = 76/321 (23.68%), Postives = 132/321 (41.12%), Query Frame = 0

Query: 129 AEEEPTKKCKDKKKTKKNAVPEMHADAAE--KEKSEP---VHVESLNNSNGTTVYEEAGK 188
           + +E + + + + +TKK A    + DA E  K K+EP      ES ++ + ++   E+G 
Sbjct: 112 SSDESSSESESEDETKKRARESDNEDAKETKKAKTEPESSSSSESSSSGSSSSSESESGS 171

Query: 189 KSKDKKKKKNKEKLETVATISDDVAVDSIGLNSDVTTIEEKVVKSKTKKKKDGRHLSDEN 248
           +S       +    ++ +    D    S   +SD ++              D    SD +
Sbjct: 172 ESDSDSSSSSSSSSDSESDSESDSQSSSSSSSSDSSS-------DSDSSSSDSSSDSDSS 231

Query: 249 SNQLNDGTNMLHEEEQNDNSKKRKRLASEDNNNHTVDEKETEDVKRRKLEGSKGCNDGVQ 308
           S+  +  ++   + + + +S       S  +++ + DE  + D      +   G +  ++
Sbjct: 232 SSSSSSSSDSDSDSDSSSDSDSSGSSDSSSSSDSSSDESTSSDSSDSDSDSDSGSSSELE 291

Query: 309 STKVDADAGNRSTINELSPQTNECVEKTAEKTSTKKALKKHSNGSTEPKTINAFQRVKVD 368
           + +  AD    S   E    +NE     +  +S  K          +      F RV   
Sbjct: 292 TKEATAD---ESKAEETPASSNESTPSASSSSSANKLNIPAGTDEIKEGQRKHFSRVDRS 351

Query: 369 AVTFADEKLADNSYWAKDFLFLICNLSYYTEQGGADTGYGAKAQEVLGQVKGRDFRHEKT 428
            + F   +L DN+Y                 +G A T +G KA E LG+V+G+DF   K 
Sbjct: 352 KINFEAWELTDNTY-----------------KGAAGT-WGEKANEKLGRVRGKDFTKNKN 404

Query: 429 KKKRGSYRGGVIDLQSHSVKF 445
           K KRGSYRGG I L+S S KF
Sbjct: 412 KMKRGSYRGGSITLESGSYKF 404

BLAST of Lsi06G012160 vs. ExPASy Swiss-Prot
Match: Q14978 (Nucleolar and coiled-body phosphoprotein 1 OS=Homo sapiens OX=9606 GN=NOLC1 PE=1 SV=2)

HSP 1 Score: 47.8 bits (112), Expect = 3.9e-04
Identity = 85/324 (26.23%), Postives = 136/324 (41.98%), Query Frame = 0

Query: 122 ADDRVPEAEEEPTKKCKDKKKTKKNAVPEMHADAAEKEKSEPVHVESLNNSNGTTVYEEA 181
           +++    +EEE TKK     K K  A   +   A +  +      +S ++S+ ++  EE 
Sbjct: 426 SEEESSSSEEEKTKKMVATTKPKATAKAALSLPAKQAPQGSR---DSSSDSDSSSSEEEE 485

Query: 182 GKKSKDKKKKKNKEKLETVATISDDVAVDSIGLNSDVTTIEEKVVKSKTKKKKDGRHLSD 241
            K SK   KKK ++     A      A      +S+ ++ ++   + + K K  G   S 
Sbjct: 486 EKTSKSAVKKKPQKVAGGAAPSKPASAKKGKAESSNSSSSDDSSEEEEEKLKGKG---SP 545

Query: 242 ENSNQLNDGTNMLHEEEQNDNSKKRKRLASEDNNNHTVDEKETEDVKRRKLEGSKGCNDG 301
                  +GT+ L    QN  + K      E+     V   ++  +K+RK          
Sbjct: 546 RPQAPKANGTSAL--TAQNGKAAKNSEEEEEEKKKAAVVVSKSGSLKKRK---------- 605

Query: 302 VQSTKVDADAGNRSTINELSPQTNECVEKTAEKTSTKKALKKHSNGSTEPKTINAFQRVK 361
                      N +     +PQ  +       K  T     K   G  E +  + F+RV+
Sbjct: 606 ----------QNEAAKEAETPQAKKI------KLQTPNTFPKRKKG--EKRASSPFRRVR 665

Query: 362 VDAVTFADEKLADNSYWAKDFLFLICNLSYYTEQGGADTGYGAKAQEVLGQVKGRDFRHE 421
            + +   D ++ADNS+ AK                GA   +G +A +VL   KG+ FRHE
Sbjct: 666 EEEIE-VDSRVADNSFDAK---------------RGAAGDWGERANQVLKFTKGKSFRHE 697

Query: 422 KTKKKRGSYRGGVIDLQSHSVKFN 446
           KTKKKRGSYRGG I +Q +S+KF+
Sbjct: 726 KTKKKRGSYRGGSISVQVNSIKFD 697

BLAST of Lsi06G012160 vs. ExPASy TrEMBL
Match: A0A1S3AWK1 (nucleolar and coiled-body phosphoprotein 1 OS=Cucumis melo OX=3656 GN=LOC103483590 PE=4 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.5e-184
Identity = 364/441 (82.54%), Postives = 384/441 (87.07%), Query Frame = 0

Query: 10  EEKGCFKPRQVLLSHHTMKQRNNDNQTPNSADEPTSLHPHQRTLLLHAVAFFLERNGFSK 69
           EEKGCFKPRQVLLSHHTMKQ NN  Q PNSADE  SLHP  RTLLLHAVAFFLERNGFSK
Sbjct: 3   EEKGCFKPRQVLLSHHTMKQSNNHTQAPNSADEALSLHPQHRTLLLHAVAFFLERNGFSK 62

Query: 70  TLKRFRSEAQIQKGSSEDLLLSLEEMCHKHLKKCSQAITTQDKPDEEMVKEVADDRVPEA 129
           TLK+FRSEAQIQKGSS+DLLLSLEEMCHKHLKKCSQAITT++KPDEE VKEVAD RVPEA
Sbjct: 63  TLKKFRSEAQIQKGSSKDLLLSLEEMCHKHLKKCSQAITTENKPDEETVKEVADGRVPEA 122

Query: 130 EEEPTKKCKDKKKTKKNAVPEMHADAAEKEKSEPVHVESLNNSNGTTVYEEAGKKSKDKK 189
           +EE TKK KDKKK KKNAVPEM+ DAAE EKSEPVH ESLNNSNGTTV+++AGKKSKDKK
Sbjct: 123 QEERTKKSKDKKKIKKNAVPEMYVDAAENEKSEPVHAESLNNSNGTTVHDDAGKKSKDKK 182

Query: 190 KKKNKEKLETVATISDDVAVDSIGLNSDVTTIEEKVVKSKTKKKKDGRHLSDENSNQLND 249
           KKKNKEKLETVATIS+D+AVDSIGLN DV ++EEKVVKSKTKKKKDGRHLSDE   +   
Sbjct: 183 KKKNKEKLETVATISNDIAVDSIGLNGDVASLEEKVVKSKTKKKKDGRHLSDEKEKE--- 242

Query: 250 GTNMLHEEEQNDNSKKRKRLASEDNNNHTVDEKETEDVKRRKLEGSKGCNDGVQSTKVDA 309
             N+   +EQND SKKRKRLASEDN+   VDEKETEDVKRRKLE SKG N  +QSTKVD 
Sbjct: 243 -QNVSVVDEQNDISKKRKRLASEDNDIPAVDEKETEDVKRRKLECSKGGNVSLQSTKVDV 302

Query: 310 DAGNRSTINELSPQTNECVEKTAEKTSTKKALKKHSNGSTEPKTINAFQRVKVDAVTFAD 369
           DAGNRS INELS QTNE VEKTAEKTSTKKA KKHSNGSTEPKTINAFQRVKVDAVTFAD
Sbjct: 303 DAGNRSAINELSQQTNESVEKTAEKTSTKKAFKKHSNGSTEPKTINAFQRVKVDAVTFAD 362

Query: 370 EKLADNSYWAKDFLFLICNLSYYTEQGGADTGYGAKAQEVLGQVKGRDFRHEKTKKKRGS 429
           EKLADNSYWAK               GGA++GYGAKAQEVLGQVKGRDFRHEKTKKKRGS
Sbjct: 363 EKLADNSYWAK---------------GGAESGYGAKAQEVLGQVKGRDFRHEKTKKKRGS 422

Query: 430 YRGGVIDLQSHSVKFNYSDDD 451
           YRGGVIDLQSHSVKFNYSDDD
Sbjct: 423 YRGGVIDLQSHSVKFNYSDDD 424

BLAST of Lsi06G012160 vs. ExPASy TrEMBL
Match: A0A5D3D1D6 (Nucleolar and coiled-body phosphoprotein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001630 PE=4 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 1.0e-172
Identity = 346/424 (81.60%), Postives = 365/424 (86.08%), Query Frame = 0

Query: 27  MKQRNNDNQTPNSADEPTSLHPHQRTLLLHAVAFFLERNGFSKTLKRFRSEAQIQKGSSE 86
           MKQ NN  Q PNSADE  SLHP  RTLLLHAVAFFLERNGFSKTLK+FRSEAQIQKGSS+
Sbjct: 1   MKQSNNHTQAPNSADEALSLHPQHRTLLLHAVAFFLERNGFSKTLKKFRSEAQIQKGSSK 60

Query: 87  DLLLSLEEMCHKHLKKCSQAITTQDKPDEEMVKEVADDRVPEAEEEPTKKCKDKKKTKKN 146
           DLLLSLEEMCHKHLKKCSQAITT++KPDEE VKEVAD RVPEA+EE TKK KDKKK KKN
Sbjct: 61  DLLLSLEEMCHKHLKKCSQAITTENKPDEETVKEVADGRVPEAQEERTKKSKDKKKIKKN 120

Query: 147 AVPEMHADAAEKEKSEPVHVESLNNSNGTTVYEEAGKKSKDKKKKKNKEKLETVATISDD 206
           AVPEM+ DAAE EKSEPVH ESLNN NGTTV++EAGKKSKDKKKKKNKEKLET ATIS+D
Sbjct: 121 AVPEMYVDAAENEKSEPVHAESLNNFNGTTVHDEAGKKSKDKKKKKNKEKLETAATISND 180

Query: 207 VAVDSIGLNSDVTTIEEKVVKSKTKKKKDGRHLSDENSNQLNDGTNMLHEEEQNDNSKKR 266
           +AVDSIGLN DV ++EEKVVKSKTKKKKDGRHLSDE   +     N+   +EQND SKKR
Sbjct: 181 IAVDSIGLNGDVASLEEKVVKSKTKKKKDGRHLSDEKEKE----QNVSVVDEQNDISKKR 240

Query: 267 KRLASEDNNNHTVDEKETEDVKRRKLEGSKGCNDGVQSTKVDADAGNRSTINELSPQTNE 326
           KRLASEDN+   VDEKETEDVKRRKLE SKG N  +QSTKVD DAGNRS INELS QTNE
Sbjct: 241 KRLASEDNDIPAVDEKETEDVKRRKLECSKGGNVSLQSTKVDVDAGNRSAINELSQQTNE 300

Query: 327 CVEKTAEKTSTKKALKKHSNGSTEPKTINAFQRVKVDAVTFADEKLADNSYWAKDFLFLI 386
            VEKTAEKTSTKKA KKHSNGSTEPKTINAFQRVKVDAVTFADEKLADNSYWAK      
Sbjct: 301 SVEKTAEKTSTKKAFKKHSNGSTEPKTINAFQRVKVDAVTFADEKLADNSYWAK------ 360

Query: 387 CNLSYYTEQGGADTGYGAKAQEVLGQVKGRDFRHEKTKKKRGSYRGGVIDLQSHSVKFNY 446
                    GGA++GYGAKAQEVLGQVKGRDFRHEKTKKKRGSYRGGVIDLQSHSVKFNY
Sbjct: 361 ---------GGAESGYGAKAQEVLGQVKGRDFRHEKTKKKRGSYRGGVIDLQSHSVKFNY 405

Query: 447 SDDD 451
           SDDD
Sbjct: 421 SDDD 405

BLAST of Lsi06G012160 vs. ExPASy TrEMBL
Match: A0A5A7U1N0 (Nucleolar and coiled-body phosphoprotein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G004900 PE=4 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 4.0e-169
Identity = 344/432 (79.63%), Postives = 363/432 (84.03%), Query Frame = 0

Query: 27  MKQRNNDNQTPNSADEPTSLHPHQRTLLLHAVAFFLERNGFSKTLKRFRSEAQIQKGSSE 86
           MKQ NN  Q PNSADE  SLHP  RTLLLHAVAFFLERNGFSKTLK+FRSEAQIQKGSS+
Sbjct: 1   MKQSNNHTQAPNSADEALSLHPQHRTLLLHAVAFFLERNGFSKTLKKFRSEAQIQKGSSK 60

Query: 87  DLLLSLEEMCHKHLKKCSQAITTQDKPDEEMVKEVADDRVPEAEEEPTKKCKDKKKTKKN 146
           DLLLSLEEMCHKHLKKCSQAITT++KPDEE VKEVAD RVPEA+EE TKK KDKKK KKN
Sbjct: 61  DLLLSLEEMCHKHLKKCSQAITTENKPDEETVKEVADGRVPEAQEERTKKSKDKKKIKKN 120

Query: 147 AVPEMHADAAEKEKSEPVHVESLNNSNGTTVYEEAGKKSKDKKKKKNKEKLETVATISDD 206
           AVPEM+ DAAE EKSEPVH ESLNN NGTTV++EAGKKSKDKKKKKNKEKLET ATIS+D
Sbjct: 121 AVPEMYVDAAENEKSEPVHAESLNNFNGTTVHDEAGKKSKDKKKKKNKEKLETAATISND 180

Query: 207 VAVDSIGLNSDVTTIEEKVVKSKTKKKKDGRHLSDENSNQLNDGTNMLHEEEQNDNSKKR 266
           +AVDSIGLN DV ++EEKVVKSKTKKKKDGRHLSDE   +     N+   +EQND SKKR
Sbjct: 181 IAVDSIGLNGDVASLEEKVVKSKTKKKKDGRHLSDEKEKE----QNVSVVDEQNDISKKR 240

Query: 267 KRLASEDNNNHTVDEKETEDVKRRKLEGSKGCNDGVQSTKVDADAGNRSTINELSPQTNE 326
           KRLASEDN+   VDEKETEDVKRRKLE SKG N  +QSTKVD DAGNRS INELS QTNE
Sbjct: 241 KRLASEDNDIPAVDEKETEDVKRRKLECSKGGNVSLQSTKVDVDAGNRSAINELSQQTNE 300

Query: 327 CVEKTAEKTSTKKALKKHSNGSTEPKTINAFQRVKVDAVTFADEKLADNSYWAKDFLFLI 386
            VEKTAEKTSTKKA KKHSNGSTEPKTINAFQRVKVDAVTFADEKLADNSYWAK      
Sbjct: 301 SVEKTAEKTSTKKAFKKHSNGSTEPKTINAFQRVKVDAVTFADEKLADNSYWAK------ 360

Query: 387 CNLSYYTEQGGADTGYGAKAQEVLGQVKG--------RDFRHEKTKKKRGSYRGGVIDLQ 446
                    GGA++GYGAKAQEVLGQ K         RDFRHEKTKKKRGSYRGGVIDLQ
Sbjct: 361 ---------GGAESGYGAKAQEVLGQKKALDSNLSCIRDFRHEKTKKKRGSYRGGVIDLQ 413

Query: 447 SHSVKFNYSDDD 451
           SHSVKFNYSDDD
Sbjct: 421 SHSVKFNYSDDD 413

BLAST of Lsi06G012160 vs. ExPASy TrEMBL
Match: A0A6J1JJ58 (nucleolar and coiled-body phosphoprotein 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484917 PE=4 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 2.7e-165
Identity = 344/452 (76.11%), Postives = 369/452 (81.64%), Query Frame = 0

Query: 15  FKPRQVLLSHHTMKQRNNDNQTPNSADEPTSLHPHQRTLLLHAVAFFLERNGFSKTLKRF 74
           FKPRQVLLSHH MKQR ND QTP SADE  SLHP QRTLLLHAVAFFLE NGFSKTLK+F
Sbjct: 16  FKPRQVLLSHHAMKQR-NDAQTPKSADEAASLHPEQRTLLLHAVAFFLEHNGFSKTLKKF 75

Query: 75  RSEAQIQKGSSEDLLLSLEEMCHKHLKKCSQAITTQDKPDEEMVKEVADDRVPEAEEEPT 134
           RSEAQI+K SS++LLLSLEEMCHKH KK SQA+T Q+KPDEE+V EV DDRVPE +EEP 
Sbjct: 76  RSEAQIEKDSSKELLLSLEEMCHKHFKKFSQAVTPQNKPDEEIVTEVTDDRVPEEQEEPA 135

Query: 135 KKCKDKKKTKKNAVPEMHADAAEKEKSEPVHVESLNNSN------GTTVYEEAGKKSKDK 194
           KK KDKKK KKN V       A+KEKSEP HVESL NSN      GTTV+EEAGKKSKDK
Sbjct: 136 KKSKDKKKIKKNTV-------ADKEKSEPAHVESLKNSNGTEPSEGTTVHEEAGKKSKDK 195

Query: 195 KKKKNKEKLETVATISDDV-------AVDSIGLNSDVTTIEEKVVKSKTKKKKDGRHLSD 254
           KKKKNKEK++TVATI DDV       AVDS GLN +V T++EKVVKSK+KKKKDGRHLSD
Sbjct: 196 KKKKNKEKIKTVATIPDDVTVARDSQAVDSNGLNGNVATLDEKVVKSKSKKKKDGRHLSD 255

Query: 255 ENSNQLNDGTNMLHEEEQNDNSKKRKRLASEDN---NNHTVDEKETEDVKRRKLEGSKGC 314
            NSNQLNDGT MLHEEE NDNSKKRKRLASEDN   ++H VD+KETEDVKRRKLEGSKGC
Sbjct: 256 TNSNQLNDGTKMLHEEEHNDNSKKRKRLASEDNDSHDSHAVDDKETEDVKRRKLEGSKGC 315

Query: 315 NDGVQSTKVDADAGNRSTINELSPQTNECVEKTAEKTSTKKALKKHSNGSTEPKTINAFQ 374
           NDGV STKVD        +NELS +T+  VEKTAEKTS+KK  KK SNGSTEPKTINAFQ
Sbjct: 316 NDGVLSTKVD--------VNELSQETD--VEKTAEKTSSKKPWKKSSNGSTEPKTINAFQ 375

Query: 375 RVKVDAVTFADEKLADNSYWAKDFLFLICNLSYYTEQGGADTGYGAKAQEVLGQVKGRDF 434
           RVKVDAVTFADEKLADNSYWAK               GGA+TGYGAKAQEVLGQVKGR F
Sbjct: 376 RVKVDAVTFADEKLADNSYWAK---------------GGAETGYGAKAQEVLGQVKGRGF 434

Query: 435 RHEKTKKKRGSYRGGVIDLQSHSVKFNYSDDD 451
           RHEKTKKKRGSYRGGVIDLQSHSVKFNYSD+D
Sbjct: 436 RHEKTKKKRGSYRGGVIDLQSHSVKFNYSDED 434

BLAST of Lsi06G012160 vs. ExPASy TrEMBL
Match: A0A6J1JA31 (nucleolar and coiled-body phosphoprotein 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484917 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 6.5e-164
Identity = 344/453 (75.94%), Postives = 369/453 (81.46%), Query Frame = 0

Query: 15  FKPRQVLLSHHTMKQRNNDNQTPNSADEPTSLHPHQRTLLLHAVAFFLERNGFSKTLKRF 74
           FKPRQVLLSHH MKQR ND QTP SADE  SLHP QRTLLLHAVAFFLE NGFSKTLK+F
Sbjct: 16  FKPRQVLLSHHAMKQR-NDAQTPKSADEAASLHPEQRTLLLHAVAFFLEHNGFSKTLKKF 75

Query: 75  RSEAQIQKGSSEDLLLSLEEMCHKHLKKCSQAITTQDKP-DEEMVKEVADDRVPEAEEEP 134
           RSEAQI+K SS++LLLSLEEMCHKH KK SQA+T Q+KP DEE+V EV DDRVPE +EEP
Sbjct: 76  RSEAQIEKDSSKELLLSLEEMCHKHFKKFSQAVTPQNKPVDEEIVTEVTDDRVPEEQEEP 135

Query: 135 TKKCKDKKKTKKNAVPEMHADAAEKEKSEPVHVESLNNSN------GTTVYEEAGKKSKD 194
            KK KDKKK KKN V       A+KEKSEP HVESL NSN      GTTV+EEAGKKSKD
Sbjct: 136 AKKSKDKKKIKKNTV-------ADKEKSEPAHVESLKNSNGTEPSEGTTVHEEAGKKSKD 195

Query: 195 KKKKKNKEKLETVATISDDV-------AVDSIGLNSDVTTIEEKVVKSKTKKKKDGRHLS 254
           KKKKKNKEK++TVATI DDV       AVDS GLN +V T++EKVVKSK+KKKKDGRHLS
Sbjct: 196 KKKKKNKEKIKTVATIPDDVTVARDSQAVDSNGLNGNVATLDEKVVKSKSKKKKDGRHLS 255

Query: 255 DENSNQLNDGTNMLHEEEQNDNSKKRKRLASEDN---NNHTVDEKETEDVKRRKLEGSKG 314
           D NSNQLNDGT MLHEEE NDNSKKRKRLASEDN   ++H VD+KETEDVKRRKLEGSKG
Sbjct: 256 DTNSNQLNDGTKMLHEEEHNDNSKKRKRLASEDNDSHDSHAVDDKETEDVKRRKLEGSKG 315

Query: 315 CNDGVQSTKVDADAGNRSTINELSPQTNECVEKTAEKTSTKKALKKHSNGSTEPKTINAF 374
           CNDGV STKVD        +NELS +T+  VEKTAEKTS+KK  KK SNGSTEPKTINAF
Sbjct: 316 CNDGVLSTKVD--------VNELSQETD--VEKTAEKTSSKKPWKKSSNGSTEPKTINAF 375

Query: 375 QRVKVDAVTFADEKLADNSYWAKDFLFLICNLSYYTEQGGADTGYGAKAQEVLGQVKGRD 434
           QRVKVDAVTFADEKLADNSYWAK               GGA+TGYGAKAQEVLGQVKGR 
Sbjct: 376 QRVKVDAVTFADEKLADNSYWAK---------------GGAETGYGAKAQEVLGQVKGRG 435

Query: 435 FRHEKTKKKRGSYRGGVIDLQSHSVKFNYSDDD 451
           FRHEKTKKKRGSYRGGVIDLQSHSVKFNYSD+D
Sbjct: 436 FRHEKTKKKRGSYRGGVIDLQSHSVKFNYSDED 435

BLAST of Lsi06G012160 vs. NCBI nr
Match: XP_038875161.1 (suppressor protein SRP40-like isoform X1 [Benincasa hispida])

HSP 1 Score: 718.4 bits (1853), Expect = 3.9e-203
Identity = 393/450 (87.33%), Postives = 411/450 (91.33%), Query Frame = 0

Query: 1   MFENNLLASEEKGCFKPRQVLLSHHTMKQRNNDNQTPNSADEPTSLHPHQRTLLLHAVAF 60
           MFENN  ASEEKGCFKPRQVLLSHHTMKQRNND QTPNS+DE  SLHP QRTLLLHAVAF
Sbjct: 1   MFENNFPASEEKGCFKPRQVLLSHHTMKQRNNDTQTPNSSDEAASLHPQQRTLLLHAVAF 60

Query: 61  FLERNGFSKTLKRFRSEAQIQKGSSEDLLLSLEEMCHKHLKKCSQAITTQDKPDEEMVKE 120
           FL+RNGFSKTLK+FRSEAQ+QKGSS+DLLL+LEEMC+KHLKKCSQAITTQ+KPDEE  KE
Sbjct: 61  FLDRNGFSKTLKKFRSEAQLQKGSSKDLLLNLEEMCYKHLKKCSQAITTQNKPDEETEKE 120

Query: 121 VADDRVPEAEEEPTKKCKDKKKTKKNAVPEMHADAAEKEKSEPVHVESLNNSNGTTVYEE 180
           VADDRVPEA+EEPTKK KDKKK KKNAVPE HADA+EKEKSEP  VESL NSNGTTV+EE
Sbjct: 121 VADDRVPEAQEEPTKKSKDKKKIKKNAVPETHADASEKEKSEP--VESLKNSNGTTVHEE 180

Query: 181 AGKKSKDKKKKKNKEKLETVATISDDVAVDSIGLNSDVTTIEEKVVKSKTKKKKDGRHLS 240
           AGK+SK KKKKKNKEKLETVATISDDVAV +IGL  DV T+EEKVVKSKTKKKKDGRHLS
Sbjct: 181 AGKESKGKKKKKNKEKLETVATISDDVAV-AIGLKGDVATLEEKVVKSKTKKKKDGRHLS 240

Query: 241 DENSNQLNDGTNMLHEEEQNDNSKKRKRLASEDNNNHTVDEKETEDVKRRKLEGSKGCND 300
           DEN+NQLNDGTNML+EEE+NDNSKKRKRLASEDN+ HTVDEKETEDVKRRKLEGSKGCND
Sbjct: 241 DENANQLNDGTNMLYEEEENDNSKKRKRLASEDNDIHTVDEKETEDVKRRKLEGSKGCND 300

Query: 301 GVQSTKVDADAGNRSTINELSPQTNECVEKTAEKTSTKKALKKHSNGSTEPKTINAFQRV 360
           GVQSTKVD DAGNRSTINELS QTN  VEKTAEKTSTKKALKKHSNGSTEPKTINAFQRV
Sbjct: 301 GVQSTKVDVDAGNRSTINELSQQTNGYVEKTAEKTSTKKALKKHSNGSTEPKTINAFQRV 360

Query: 361 KVDAVTFADEKLADNSYWAKDFLFLICNLSYYTEQGGADTGYGAKAQEVLGQVKGRDFRH 420
           KVDAVTFADEKLADNSYWAK               GGADTGYGAKAQEVLGQVKGRDFRH
Sbjct: 361 KVDAVTFADEKLADNSYWAK---------------GGADTGYGAKAQEVLGQVKGRDFRH 420

Query: 421 EKTKKKRGSYRGGVIDLQSHSVKFNYSDDD 451
           EKTKKKRGSYRGGVIDLQSHSVKFNYSDDD
Sbjct: 421 EKTKKKRGSYRGGVIDLQSHSVKFNYSDDD 432

BLAST of Lsi06G012160 vs. NCBI nr
Match: XP_038875166.1 (suppressor protein SRP40-like isoform X2 [Benincasa hispida])

HSP 1 Score: 712.2 bits (1837), Expect = 2.8e-201
Identity = 392/450 (87.11%), Postives = 410/450 (91.11%), Query Frame = 0

Query: 1   MFENNLLASEEKGCFKPRQVLLSHHTMKQRNNDNQTPNSADEPTSLHPHQRTLLLHAVAF 60
           MFENN  ASEEKGCFKPRQVLLSHHTMKQRNND QTPNS+DE  SLHP QRTLLLHAVAF
Sbjct: 1   MFENNFPASEEKGCFKPRQVLLSHHTMKQRNNDTQTPNSSDEAASLHPQQRTLLLHAVAF 60

Query: 61  FLERNGFSKTLKRFRSEAQIQKGSSEDLLLSLEEMCHKHLKKCSQAITTQDKPDEEMVKE 120
           FL+RNGFSKTLK+FRSEAQ+Q GSS+DLLL+LEEMC+KHLKKCSQAITTQ+KPDEE  KE
Sbjct: 61  FLDRNGFSKTLKKFRSEAQLQ-GSSKDLLLNLEEMCYKHLKKCSQAITTQNKPDEETEKE 120

Query: 121 VADDRVPEAEEEPTKKCKDKKKTKKNAVPEMHADAAEKEKSEPVHVESLNNSNGTTVYEE 180
           VADDRVPEA+EEPTKK KDKKK KKNAVPE HADA+EKEKSEP  VESL NSNGTTV+EE
Sbjct: 121 VADDRVPEAQEEPTKKSKDKKKIKKNAVPETHADASEKEKSEP--VESLKNSNGTTVHEE 180

Query: 181 AGKKSKDKKKKKNKEKLETVATISDDVAVDSIGLNSDVTTIEEKVVKSKTKKKKDGRHLS 240
           AGK+SK KKKKKNKEKLETVATISDDVAV +IGL  DV T+EEKVVKSKTKKKKDGRHLS
Sbjct: 181 AGKESKGKKKKKNKEKLETVATISDDVAV-AIGLKGDVATLEEKVVKSKTKKKKDGRHLS 240

Query: 241 DENSNQLNDGTNMLHEEEQNDNSKKRKRLASEDNNNHTVDEKETEDVKRRKLEGSKGCND 300
           DEN+NQLNDGTNML+EEE+NDNSKKRKRLASEDN+ HTVDEKETEDVKRRKLEGSKGCND
Sbjct: 241 DENANQLNDGTNMLYEEEENDNSKKRKRLASEDNDIHTVDEKETEDVKRRKLEGSKGCND 300

Query: 301 GVQSTKVDADAGNRSTINELSPQTNECVEKTAEKTSTKKALKKHSNGSTEPKTINAFQRV 360
           GVQSTKVD DAGNRSTINELS QTN  VEKTAEKTSTKKALKKHSNGSTEPKTINAFQRV
Sbjct: 301 GVQSTKVDVDAGNRSTINELSQQTNGYVEKTAEKTSTKKALKKHSNGSTEPKTINAFQRV 360

Query: 361 KVDAVTFADEKLADNSYWAKDFLFLICNLSYYTEQGGADTGYGAKAQEVLGQVKGRDFRH 420
           KVDAVTFADEKLADNSYWAK               GGADTGYGAKAQEVLGQVKGRDFRH
Sbjct: 361 KVDAVTFADEKLADNSYWAK---------------GGADTGYGAKAQEVLGQVKGRDFRH 420

Query: 421 EKTKKKRGSYRGGVIDLQSHSVKFNYSDDD 451
           EKTKKKRGSYRGGVIDLQSHSVKFNYSDDD
Sbjct: 421 EKTKKKRGSYRGGVIDLQSHSVKFNYSDDD 431

BLAST of Lsi06G012160 vs. NCBI nr
Match: XP_038880606.1 (nucleolar and coiled-body phosphoprotein 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 688.3 bits (1775), Expect = 4.3e-194
Identity = 378/441 (85.71%), Postives = 399/441 (90.48%), Query Frame = 0

Query: 10  EEKGCFKPRQVLLSHHTMKQRNNDNQTPNSADEPTSLHPHQRTLLLHAVAFFLERNGFSK 69
           +EKGCFKPRQVLLSH TMKQRNNDNQ PNSADE TSLHPHQRTLLLHAVAFFLERN FSK
Sbjct: 3   DEKGCFKPRQVLLSHRTMKQRNNDNQIPNSADEATSLHPHQRTLLLHAVAFFLERNSFSK 62

Query: 70  TLKRFRSEAQIQKGSSEDLLLSLEEMCHKHLKKCSQAITTQDKPDEEMVKEVADDRVPEA 129
           TLK+FRSEAQIQK SS+DLLLSLEEMCHKHLKKCSQ ITTQ+KPDEE VKEVADDRVPEA
Sbjct: 63  TLKKFRSEAQIQKDSSKDLLLSLEEMCHKHLKKCSQTITTQNKPDEETVKEVADDRVPEA 122

Query: 130 EEEPTKKCKDKKKTKKNAVPEMHADAAEKEKSEPVHVESLNNSNGTTVYEEAGKKSKDKK 189
           ++E TKK KDKKK KKNAVPE  ADAAEKEKSEP+HVESL N+NG+TV+EEAGKKSKDKK
Sbjct: 123 QDESTKKSKDKKKIKKNAVPETDADAAEKEKSEPIHVESLKNNNGSTVHEEAGKKSKDKK 182

Query: 190 KKKNKEKLETVATISDDVAVDSIGLNSDVTTIEEKVVKSKTKKKKDGRHLSDENSNQLND 249
           KKKNKEKLETVATIS + AVDSIGLN DV T+EEKVVKSKT+KKKDGR  SDENSNQLND
Sbjct: 183 KKKNKEKLETVATISVNAAVDSIGLNGDVATLEEKVVKSKTRKKKDGRP-SDENSNQLND 242

Query: 250 GTNMLHEEEQNDNSKKRKRLASEDNNNHTVDEKETEDVKRRKLEGSKGCNDGVQSTKVDA 309
           GT+ML+EEEQNDN+KKRKRLA EDN +HTVD+KETEDVKRRKLEG  GCNDGVQSTKVD 
Sbjct: 243 GTDMLNEEEQNDNTKKRKRLAPEDNGDHTVDDKETEDVKRRKLEG--GCNDGVQSTKVDV 302

Query: 310 DAGNRSTINELSPQTNECVEKTAEKTSTKKALKKHSNGSTEPKTINAFQRVKVDAVTFAD 369
           DAG+RSTINELS QTNE VEKTAEKTSTKKALKK SNGSTEPKTINAFQRVK+DAVTFAD
Sbjct: 303 DAGSRSTINELSQQTNEYVEKTAEKTSTKKALKKRSNGSTEPKTINAFQRVKLDAVTFAD 362

Query: 370 EKLADNSYWAKDFLFLICNLSYYTEQGGADTGYGAKAQEVLGQVKGRDFRHEKTKKKRGS 429
           EKLADNSYWAK               GGA+TGYGAKAQEVLGQVKGRDFRHEKTKKKRGS
Sbjct: 363 EKLADNSYWAK---------------GGAETGYGAKAQEVLGQVKGRDFRHEKTKKKRGS 422

Query: 430 YRGGVIDLQSHSVKFNYSDDD 451
           YRGGVIDLQSHSVKFNYSDDD
Sbjct: 423 YRGGVIDLQSHSVKFNYSDDD 425

BLAST of Lsi06G012160 vs. NCBI nr
Match: XP_038880605.1 (nucleolar and coiled-body phosphoprotein 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 683.7 bits (1763), Expect = 1.1e-192
Identity = 378/442 (85.52%), Postives = 399/442 (90.27%), Query Frame = 0

Query: 10  EEKGCFKPRQVLLSHHTMKQRNNDNQTPNSADEPTSLHPHQRTLLLHAVAFFLERNGFSK 69
           +EKGCFKPRQVLLSH TMKQRNNDNQ PNSADE TSLHPHQRTLLLHAVAFFLERN FSK
Sbjct: 3   DEKGCFKPRQVLLSHRTMKQRNNDNQIPNSADEATSLHPHQRTLLLHAVAFFLERNSFSK 62

Query: 70  TLKRFRSEAQIQKGSSEDLLLSLEEMCHKHLKKCSQAITTQDKP-DEEMVKEVADDRVPE 129
           TLK+FRSEAQIQK SS+DLLLSLEEMCHKHLKKCSQ ITTQ+KP DEE VKEVADDRVPE
Sbjct: 63  TLKKFRSEAQIQKDSSKDLLLSLEEMCHKHLKKCSQTITTQNKPVDEETVKEVADDRVPE 122

Query: 130 AEEEPTKKCKDKKKTKKNAVPEMHADAAEKEKSEPVHVESLNNSNGTTVYEEAGKKSKDK 189
           A++E TKK KDKKK KKNAVPE  ADAAEKEKSEP+HVESL N+NG+TV+EEAGKKSKDK
Sbjct: 123 AQDESTKKSKDKKKIKKNAVPETDADAAEKEKSEPIHVESLKNNNGSTVHEEAGKKSKDK 182

Query: 190 KKKKNKEKLETVATISDDVAVDSIGLNSDVTTIEEKVVKSKTKKKKDGRHLSDENSNQLN 249
           KKKKNKEKLETVATIS + AVDSIGLN DV T+EEKVVKSKT+KKKDGR  SDENSNQLN
Sbjct: 183 KKKKNKEKLETVATISVNAAVDSIGLNGDVATLEEKVVKSKTRKKKDGRP-SDENSNQLN 242

Query: 250 DGTNMLHEEEQNDNSKKRKRLASEDNNNHTVDEKETEDVKRRKLEGSKGCNDGVQSTKVD 309
           DGT+ML+EEEQNDN+KKRKRLA EDN +HTVD+KETEDVKRRKLEG  GCNDGVQSTKVD
Sbjct: 243 DGTDMLNEEEQNDNTKKRKRLAPEDNGDHTVDDKETEDVKRRKLEG--GCNDGVQSTKVD 302

Query: 310 ADAGNRSTINELSPQTNECVEKTAEKTSTKKALKKHSNGSTEPKTINAFQRVKVDAVTFA 369
            DAG+RSTINELS QTNE VEKTAEKTSTKKALKK SNGSTEPKTINAFQRVK+DAVTFA
Sbjct: 303 VDAGSRSTINELSQQTNEYVEKTAEKTSTKKALKKRSNGSTEPKTINAFQRVKLDAVTFA 362

Query: 370 DEKLADNSYWAKDFLFLICNLSYYTEQGGADTGYGAKAQEVLGQVKGRDFRHEKTKKKRG 429
           DEKLADNSYWAK               GGA+TGYGAKAQEVLGQVKGRDFRHEKTKKKRG
Sbjct: 363 DEKLADNSYWAK---------------GGAETGYGAKAQEVLGQVKGRDFRHEKTKKKRG 422

Query: 430 SYRGGVIDLQSHSVKFNYSDDD 451
           SYRGGVIDLQSHSVKFNYSDDD
Sbjct: 423 SYRGGVIDLQSHSVKFNYSDDD 426

BLAST of Lsi06G012160 vs. NCBI nr
Match: XP_004134062.1 (nucleolar and coiled-body phosphoprotein 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 669.8 bits (1727), Expect = 1.6e-188
Identity = 370/441 (83.90%), Postives = 391/441 (88.66%), Query Frame = 0

Query: 10  EEKGCFKPRQVLLSHHTMKQRNNDNQTPNSADEPTSLHPHQRTLLLHAVAFFLERNGFSK 69
           EE+GCFKPRQVLLSHHTMKQ NN  Q PNSADE  SLHP  RTLLLHAVAFFLERNGFSK
Sbjct: 3   EEQGCFKPRQVLLSHHTMKQSNNHTQAPNSADETLSLHPQHRTLLLHAVAFFLERNGFSK 62

Query: 70  TLKRFRSEAQIQKGSSEDLLLSLEEMCHKHLKKCSQAITTQDKPDEEMVKEVADDRVPEA 129
           TLK+FRSEAQI+KGSS+DLLLSLEEMCHKHLKKCSQAITT++KPDEE VKEVAD RVPEA
Sbjct: 63  TLKKFRSEAQIEKGSSKDLLLSLEEMCHKHLKKCSQAITTENKPDEETVKEVADGRVPEA 122

Query: 130 EEEPTKKCKDKKKTKKNAVPEMHADAAEKEKSEPVHVESLNNSNGTTVYEEAGKKSKDKK 189
           +EEPTKK KDKKK KKNAVPE H DAAEKEKSEP  VESL NSN TTV++EAGKKSKDKK
Sbjct: 123 QEEPTKKSKDKKKIKKNAVPETHVDAAEKEKSEP--VESLKNSNDTTVHDEAGKKSKDKK 182

Query: 190 KKKNKEKLETVATISDDVAVDSIGLNSDVTTIEEKVVKSKTKKKKDGRHLSDENSNQLND 249
           KKKNKEKLETVATIS+D+AVDSIGLN DV ++EEKVVKSKTKKKKDGRH S ENSNQLND
Sbjct: 183 KKKNKEKLETVATISNDIAVDSIGLNGDVASLEEKVVKSKTKKKKDGRHSSHENSNQLND 242

Query: 250 GTNMLHEEEQNDNSKKRKRLASEDNNNHTVDEKETEDVKRRKLEGSKGCNDGVQSTKVDA 309
           GTN+L+EEEQND SKKRKRLASEDN+   V+EK TEDVKRRKLE SKG +D VQSTKVD 
Sbjct: 243 GTNILNEEEQNDISKKRKRLASEDNDIPAVNEKATEDVKRRKLECSKGGSDSVQSTKVDV 302

Query: 310 DAGNRSTINELSPQTNECVEKTAEKTSTKKALKKHSNGSTEPKTINAFQRVKVDAVTFAD 369
           DAGNRSTINE+SPQTNE VEKTAEKTSTKKA KKHSNGSTEPKTIN FQRVKVDAVTFAD
Sbjct: 303 DAGNRSTINEVSPQTNEYVEKTAEKTSTKKAFKKHSNGSTEPKTINPFQRVKVDAVTFAD 362

Query: 370 EKLADNSYWAKDFLFLICNLSYYTEQGGADTGYGAKAQEVLGQVKGRDFRHEKTKKKRGS 429
           EKLADNSYWAK               GGA++GYGAKAQEVLGQVKGR FRHEKTKKKRGS
Sbjct: 363 EKLADNSYWAK---------------GGAESGYGAKAQEVLGQVKGRGFRHEKTKKKRGS 422

Query: 430 YRGGVIDLQSHSVKFNYSDDD 451
           YRGGVIDLQSHSVKFNYSDDD
Sbjct: 423 YRGGVIDLQSHSVKFNYSDDD 426

BLAST of Lsi06G012160 vs. TAIR 10
Match: AT5G57120.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: nucleolus; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: LisH dimerisation motif (InterPro:IPR006594), SRP40, C-terminal (InterPro:IPR007718); Has 101969 Blast hits to 55488 proteins in 2506 species: Archae - 424; Bacteria - 13843; Metazoa - 37674; Fungi - 9726; Plants - 4941; Viruses - 569; Other Eukaryotes - 34792 (source: NCBI BLink). )

HSP 1 Score: 151.0 bits (380), Expect = 2.3e-36
Identity = 148/407 (36.36%), Postives = 198/407 (48.65%), Query Frame = 0

Query: 44  TSLHPHQRTLLLHAVAFFLERNGFSKTLKRFRSEAQIQKGSSEDLLLSLEEMCHKHLKKC 103
           ++L   Q+ LLL +VA +LER GFSK  K+  SEA+I+K      L  LEE+  + L K 
Sbjct: 8   SALESEQKALLLRSVAQYLERCGFSKCFKKLLSEAEIEKKELNTSLPDLEEIFSEFLNKR 67

Query: 104 SQAITTQDKPDEEMVKEVADDRVPEAEEEPTKKCKDKKKTKKNAVPEMHADAAEKEKSEP 163
                     +  +V+ V          E  KK K KKK K+  V     +  E+EK + 
Sbjct: 68  DHEAAANGNTEANVVEAV----------ENVKKDKKKKKNKETKV-----EVTEEEKVKE 127

Query: 164 VHVESLNNSNGTTVYEEAGKKSKDKKKKKNKEKLETVATISDDVAVDSIGLNSDVTTIEE 223
                      T    E G K K KKKK+ K K+     + +  AV           IE+
Sbjct: 128 -----------TDAVIEDGVKEK-KKKKETKVKVTEEEKVKETDAV-----------IED 187

Query: 224 KVVKSKTKKKKDGRHLSDENSNQLNDGTNMLHEEEQNDNSKKRKRLASEDNNNHTVDEKE 283
            V + K KK K     +D++  ++               SKKRKR   E+    T D+ E
Sbjct: 188 GVKEKKKKKSKSKSVEADDDKEKV---------------SKKRKRSEPEETKEETEDDDE 247

Query: 284 TEDVKRRKLEGSKGCNDGVQSTKVDADAGNRSTINELSPQTNECVEKTAEKTSTKKALKK 343
            E  +R+K E     ++GVQ T V           E   + N   EK+  K++ +K+ K 
Sbjct: 248 -ESKRRKKEENVVENDEGVQETPV----------KETETKENGNAEKSETKSTNQKSGKG 307

Query: 344 HSNGSTEPKTINAFQRVKVDAVTFADEKLADNSYWAKDFLFLICNLSYYTEQGGADTGYG 403
            SN S EPK    FQRV VD + + +     NSY++K               GGA+ GYG
Sbjct: 308 LSN-SKEPK--KPFQRVNVDEIVYTEN---SNSYYSK---------------GGAEIGYG 329

Query: 404 AKAQEVLGQVKGRDFRHEKTKKKRGSYRGGVIDLQSHSVKFNYSDDD 451
            KAQEVLGQV+GRDFRHEKTKKKRGSYRGG+ID +SHS KFN SDD+
Sbjct: 368 LKAQEVLGQVRGRDFRHEKTKKKRGSYRGGLIDQESHSTKFNNSDDE 329