Lsi06G011300 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G011300
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionXyloglucan endotransglucosylase/hydrolase
Locationchr06: 21558709 .. 21560343 (+)
RNA-Seq ExpressionLsi06G011300
SyntenyLsi06G011300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAATTAAAACATCTTCAATTTCTTCTTCTTCTTCAAAATTTGCCATTCATGTGTTATTTCTTTGTGTTATGATCATCTTTAGCTCTTCAATGGCTGCCTCTGCCAACTTCTTGAAGGACATTGACCACACATGGGGAGGAGGTGACAGAGTTCAAATACTCAACAATGGCGAAGGCCTTGCTATCGCCCTTGACGAGACCTCCTGTTCCGGATTCCAATCTCGAGACCAATACCTCTTTGCCAAGATTGATTTGCAAATCAAACTTGTTCCGGGAAATTCGGCTGGCACAGTCACTGCATTTTACGTGAGTGTGATAATATATATTTTGAGTTGATTAGTGATTTAAAGATAGTATCAAAGTACGTAATATAATTAAAATTACTTTTTCGTGTTTAGGTGTCGTCTAAAGGAGAGTTTCATGACGAGATAGACTTTGAATTCTTGGGAAGAGTTGAGGAGGATCCTTATATTTTGCAAACAAACATGTTCATCCATGGTGTTGGCAAAAGAGAGACACAATTTTTCCTTTGGTTTGACCCAACAGCTGATTTTCACAATTACACTATTCTTTGGACGCCTATTCATATCGTGTGAGTATTATCAAAAACCTAATTAAACACATATGTTCAAATACTATTTTGATTTTGATTGAATATGATCACCAAATTTTGGAAAAAAAAAAAACTATCAATTTCTATCTTAAACTTTTCAGTATTAACTACATATGACTACTAACTAAAATCTCATAAAATTCAAATATTAAATACCAAATATTTATGATTTACAAACTTTAAACAAAGGCAAATAACCATTCTGAAAGTAAAATATTAAAAATTCACAAATTCGTAAATCCAAAGTAATCAAATTTTAAACAAAAAGATATATATATATATATATAATTCGGATTGAATTAGGTCAAACTAACTATATGTTTTTTTTAGTGAAATTGCGACCTAACTTAACCTAAGAAAAATTGAAAAAATTTAACCCAACCCAAAAACAAAACCAACCCAACCCAACCCTTACATTTTGGGTTGAATAGGTTGGAGTTATCAAGTTATTTAAACACCCATAATCAAAATACTCAACTAGCCCTAATGGTTTGTTTGAAAGTTAATTTTACGAAATGTAATGAGAATTAATGTTGAAAAGAGTATTTTTAGGAGGATGGATTAAAATCATATATATCAAACTATAAAATGAAGAGTATTTAATGTTGAATAATTGTGACAGGCTGTACGTTGACGGGATTCCGATAAGAGAGTTGAAAAACAAGAAAGAAAAGGGAGCTCCATTCCCAATCGGACAGCCAATGAGAATATACGGCAGCCTATGGAACGCAGACAGCTGGGCCACAAGAGGTGGGGCGGTCAAGATAGACTGGACCAAGGCGCCGTTCCGAGCTTGGTTCAAGAATCTAAGGGTCGATGGTTGCTTGTGGTCGGATGGCCGGAAGTCCAATCATTCCAATTGTACAACTAAGGCTTCAACTTCTTGGCTCTCCTCCACCGGCCTTGATAATGAAAGCCAACAGCACATGAAATGGGCTCAAACTAACTATATGTTCTATGATTACTGCACTGATATCAAACGTTTCCCCAAGGGTCTCCCTCTTGAGTGTTTATATTAA

mRNA sequence

ATGGAAATTAAAACATCTTCAATTTCTTCTTCTTCTTCAAAATTTGCCATTCATGTGTTATTTCTTTGTGTTATGATCATCTTTAGCTCTTCAATGGCTGCCTCTGCCAACTTCTTGAAGGACATTGACCACACATGGGGAGGAGGTGACAGAGTTCAAATACTCAACAATGGCGAAGGCCTTGCTATCGCCCTTGACGAGACCTCCTGTTCCGGATTCCAATCTCGAGACCAATACCTCTTTGCCAAGATTGATTTGCAAATCAAACTTGTTCCGGGAAATTCGGCTGGCACAGTCACTGCATTTTACGTGTCGTCTAAAGGAGAGTTTCATGACGAGATAGACTTTGAATTCTTGGGAAGAGTTGAGGAGGATCCTTATATTTTGCAAACAAACATGTTCATCCATGGTGTTGGCAAAAGAGAGACACAATTTTTCCTTTGGTTTGACCCAACAGCTGATTTTCACAATTACACTATTCTTTGGACGCCTATTCATATCGTGCTGTACGTTGACGGGATTCCGATAAGAGAGTTGAAAAACAAGAAAGAAAAGGGAGCTCCATTCCCAATCGGACAGCCAATGAGAATATACGGCAGCCTATGGAACGCAGACAGCTGGGCCACAAGAGGTGGGGCGGTCAAGATAGACTGGACCAAGGCGCCGTTCCGAGCTTGGTTCAAGAATCTAAGGGTCGATGGTTGCTTGTGGTCGGATGGCCGGAAGTCCAATCATTCCAATTGTACAACTAAGGCTTCAACTTCTTGGCTCTCCTCCACCGGCCTTGATAATGAAAGCCAACAGCACATGAAATGGGCTCAAACTAACTATATGTTCTATGATTACTGCACTGATATCAAACGTTTCCCCAAGGGTCTCCCTCTTGAGTGTTTATATTAA

Coding sequence (CDS)

ATGGAAATTAAAACATCTTCAATTTCTTCTTCTTCTTCAAAATTTGCCATTCATGTGTTATTTCTTTGTGTTATGATCATCTTTAGCTCTTCAATGGCTGCCTCTGCCAACTTCTTGAAGGACATTGACCACACATGGGGAGGAGGTGACAGAGTTCAAATACTCAACAATGGCGAAGGCCTTGCTATCGCCCTTGACGAGACCTCCTGTTCCGGATTCCAATCTCGAGACCAATACCTCTTTGCCAAGATTGATTTGCAAATCAAACTTGTTCCGGGAAATTCGGCTGGCACAGTCACTGCATTTTACGTGTCGTCTAAAGGAGAGTTTCATGACGAGATAGACTTTGAATTCTTGGGAAGAGTTGAGGAGGATCCTTATATTTTGCAAACAAACATGTTCATCCATGGTGTTGGCAAAAGAGAGACACAATTTTTCCTTTGGTTTGACCCAACAGCTGATTTTCACAATTACACTATTCTTTGGACGCCTATTCATATCGTGCTGTACGTTGACGGGATTCCGATAAGAGAGTTGAAAAACAAGAAAGAAAAGGGAGCTCCATTCCCAATCGGACAGCCAATGAGAATATACGGCAGCCTATGGAACGCAGACAGCTGGGCCACAAGAGGTGGGGCGGTCAAGATAGACTGGACCAAGGCGCCGTTCCGAGCTTGGTTCAAGAATCTAAGGGTCGATGGTTGCTTGTGGTCGGATGGCCGGAAGTCCAATCATTCCAATTGTACAACTAAGGCTTCAACTTCTTGGCTCTCCTCCACCGGCCTTGATAATGAAAGCCAACAGCACATGAAATGGGCTCAAACTAACTATATGTTCTATGATTACTGCACTGATATCAAACGTTTCCCCAAGGGTCTCCCTCTTGAGTGTTTATATTAA

Protein sequence

MEIKTSSISSSSSKFAIHVLFLCVMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGEGLAIALDETSCSGFQSRDQYLFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLGRVEEDPYILQTNMFIHGVGKRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIRELKNKKEKGAPFPIGQPMRIYGSLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDGRKSNHSNCTTKASTSWLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLECLY
Homology
BLAST of Lsi06G011300 vs. ExPASy Swiss-Prot
Match: Q38857 (Xyloglucan endotransglucosylase/hydrolase protein 22 OS=Arabidopsis thaliana OX=3702 GN=XTH22 PE=1 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 9.9e-89
Identity = 159/283 (56.18%), Postives = 204/283 (72.08%), Query Frame = 0

Query: 18  HVLFLCVMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGEGLAIALDETSCSGFQSRD 77
           ++L L + +I +SS+  SANF +D++ TWG G R QI NNGE L ++LD++S SGFQS++
Sbjct: 5   YLLPLFLSLIITSSV--SANFQRDVEITWGDG-RGQIKNNGELLTLSLDKSSGSGFQSKN 64

Query: 78  QYLFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLGRVEEDPYILQTNMFIHG 137
           +YLF K+ +Q+KLVPGNSAGTVT  Y+ S G   DEIDFEFLG    +PY L TN++  G
Sbjct: 65  EYLFGKVSMQMKLVPGNSAGTVTTLYLKSPGTTWDEIDFEFLGNSSGEPYTLHTNVYTQG 124

Query: 138 VGKRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIRELKNKKEKGAPFPIGQPMRI 197
            G +E QF LWFDPTA+FH YTILW P  I+  VDG PIRE KN +  G  FP  +PMR+
Sbjct: 125 KGDKEQQFKLWFDPTANFHTYTILWNPQRIIFTVDGTPIREFKNMESLGTLFPKNKPMRM 184

Query: 198 YGSLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDGRKSNHSNCTTKAST--S 257
           Y SLWNAD WATRGG VK DW+KAPF A ++  + + C+WS+G KS+  N + + +T  S
Sbjct: 185 YSSLWNADDWATRGGLVKTDWSKAPFTASYRGFQQEACVWSNG-KSSCPNASKQGTTTGS 244

Query: 258 WLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLECL 299
           WLS   LD+ +QQ M+W Q NYM Y+YCTD KRFP+GLP ECL
Sbjct: 245 WLSQE-LDSTAQQRMRWVQRNYMIYNYCTDAKRFPQGLPKECL 282

BLAST of Lsi06G011300 vs. ExPASy Swiss-Prot
Match: Q38910 (Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis thaliana OX=3702 GN=XTH23 PE=2 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.1e-87
Identity = 155/277 (55.96%), Postives = 196/277 (70.76%), Query Frame = 0

Query: 24  VMIIFSSSM--AASANFLKDIDHTWGGGDRVQILNNGEGLAIALDETSCSGFQSRDQYLF 83
           V+ + +S M  + SANF +D++ TWG G R QI NNG+ L ++LD+ S SGFQS+++YLF
Sbjct: 10  VVALLASFMICSVSANFQRDVEITWGDG-RGQITNNGDLLTLSLDKASGSGFQSKNEYLF 69

Query: 84  AKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLGRVEEDPYILQTNMFIHGVGKR 143
            KID+QIKLV GNSAGTVTA+Y+ S G   DEIDFEFLG +  DPY L TN+F  G G R
Sbjct: 70  GKIDMQIKLVAGNSAGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKGDR 129

Query: 144 ETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIRELKNKKEKGAPFPIGQPMRIYGSL 203
           E QF LWFDPT+DFH Y+ILW P  I+  VDG PIRE KN + +G  FP  QPMR+Y SL
Sbjct: 130 EQQFKLWFDPTSDFHTYSILWNPQRIIFSVDGTPIREFKNMESQGTLFPKNQPMRMYSSL 189

Query: 204 WNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDGRKSNHSNCTTKASTSWLSSTG 263
           WNA+ WATRGG VK DW+KAPF A ++    + C+  +G+ S  +     ++ SWLS   
Sbjct: 190 WNAEEWATRGGLVKTDWSKAPFTASYRGFNEEACVVINGQSSCPNVSGQGSTGSWLSQE- 249

Query: 264 LDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLECL 299
           LD+  Q+ M+W Q NYM Y+YCTD KRFP+GLP ECL
Sbjct: 250 LDSTGQEQMRWVQNNYMIYNYCTDAKRFPQGLPRECL 284

BLAST of Lsi06G011300 vs. ExPASy Swiss-Prot
Match: Q38907 (Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis thaliana OX=3702 GN=XTH25 PE=2 SV=2)

HSP 1 Score: 312.4 bits (799), Expect = 5.6e-84
Identity = 155/286 (54.20%), Postives = 191/286 (66.78%), Query Frame = 0

Query: 13  SKFAIHVLF-LCVMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGEGLAIALDETSCS 72
           S F + +LF L V      S   +  F  + D TWG G R ++LNNGE L ++LD  S S
Sbjct: 4   STFILSLLFTLTVSTTTLFSPVFAGTFDTEFDITWGDG-RGKVLNNGELLTLSLDRASGS 63

Query: 73  GFQSRDQYLFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLGRVEEDPYILQT 132
           GFQ++ +YLF KID+Q+KLVPGNSAGTVTA+Y+ SKG+  DEIDFEFLG +  DPY + T
Sbjct: 64  GFQTKKEYLFGKIDMQLKLVPGNSAGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHT 123

Query: 133 NMFIHGVGKRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIRELKNKKEKGAPFPI 192
           N++  G G RE QF LWFDPTADFH Y++LW P HIV  VD IP+RE KN +  G  +P 
Sbjct: 124 NVYTQGKGDREQQFHLWFDPTADFHTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPK 183

Query: 193 GQPMRIYGSLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDGRKSNHSNCTTK 252
            QPMR+Y SLWNAD WATRGG VK DW+KAPF A ++N R D C+ S GR    S+C   
Sbjct: 184 LQPMRLYSSLWNADQWATRGGLVKTDWSKAPFTASYRNFRADACVSSGGR----SSCPA- 243

Query: 253 ASTSWLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
            S  W S   LD  ++  M+  Q  YM Y+YCTD KRFP+G P EC
Sbjct: 244 GSPRWFSQR-LDLTAEDKMRVVQRKYMIYNYCTDTKRFPQGFPKEC 282

BLAST of Lsi06G011300 vs. ExPASy Swiss-Prot
Match: P35694 (Xyloglucan endotransglucosylase/hydrolase 2 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 7.3e-84
Identity = 148/288 (51.39%), Postives = 197/288 (68.40%), Query Frame = 0

Query: 10  SSSSKFAIHVLFLCVMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGEGLAIALDETS 69
           SS+     +VL L  +++ +     + +F +D D TW GGDR +I N G+ L+++LD+ S
Sbjct: 4   SSAHNNGFYVLMLVGIVVSTMVATCAGSFYQDFDLTW-GGDRAKIFNGGQLLSLSLDKVS 63

Query: 70  CSGFQSRDQYLFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLGRVEEDPYIL 129
            SGF+S+ +YLF +ID+Q+KLV GNSAGTVTA+Y+SS+G  HDEIDFEFLG +  DPYIL
Sbjct: 64  GSGFKSKKEYLFGRIDMQLKLVAGNSAGTVTAYYLSSQGPTHDEIDFEFLGNLSGDPYIL 123

Query: 130 QTNMFIHGVGKRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIRELKNKKEKGAPF 189
            TN+F  G G RE QF+LWFDPT +FH Y+I+W P HI+  VD  PIR  KN +  G PF
Sbjct: 124 HTNIFTQGKGNREQQFYLWFDPTRNFHTYSIIWKPQHIIFLVDNTPIRVFKNAEPLGVPF 183

Query: 190 PIGQPMRIYGSLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDGRKSNHSNCT 249
           P  QPMRIY SLWNAD WATRGG VK DW+KAPF A+++N +          KS+ SN  
Sbjct: 184 PKNQPMRIYSSLWNADDWATRGGLVKTDWSKAPFTAYYRNFKA----IEFSSKSSISN-- 243

Query: 250 TKASTSWLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
              S +   +  LD  S++ ++W Q  +M Y+YC+D+KRFP+GLP EC
Sbjct: 244 ---SGAEYEANELDAYSRRRLRWVQKYFMIYNYCSDLKRFPQGLPAEC 281

BLAST of Lsi06G011300 vs. ExPASy Swiss-Prot
Match: Q9ZV40 (Probable xyloglucan endotransglucosylase/hydrolase protein 21 OS=Arabidopsis thaliana OX=3702 GN=XTH21 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.6e-83
Identity = 154/288 (53.47%), Postives = 197/288 (68.40%), Query Frame = 0

Query: 20  LFLCVMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGEGLAIALDETSCSGFQSRDQY 79
           LFL + I+    +    +F +DID TWG G R  ILNNG  L + LD++S SGFQS+ +Y
Sbjct: 13  LFLGLSILL---VVHGKDFNQDIDITWGDG-RGNILNNGTLLNLGLDQSSGSGFQSKAEY 72

Query: 80  LFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLGRVEEDPYILQTNMFIHGVG 139
           L+ K+D+QIKLVPGNSAGTVT FY+ S+G   DEIDFEFLG V  DPYI+ TN++  G G
Sbjct: 73  LYGKVDMQIKLVPGNSAGTVTTFYLKSQGLTWDEIDFEFLGNVSGDPYIVHTNVYTQGKG 132

Query: 140 KRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIRELKNKKEKGAPFPIGQPMRIYG 199
            RE QF+LWFDPTA FHNY+ILW P HIV Y+DG PIRE KN +  G  +P  QPMR+YG
Sbjct: 133 DREQQFYLWFDPTAAFHNYSILWNPSHIVFYIDGKPIREFKNLEVLGVAYPKNQPMRMYG 192

Query: 200 SLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVD-GCLWS--DGRKS------NHSNCTT 259
           SLWNAD WATRGG VK +W++ PF A F N   +  C+WS  +G  +        S  ++
Sbjct: 193 SLWNADDWATRGGLVKTNWSQGPFVASFMNYNSENACVWSIVNGTTTTSPCSPGDSTSSS 252

Query: 260 KASTS-WLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
            +STS W S  G+D+ S++ ++W Q  +M Y+YC D KRF  GLP+EC
Sbjct: 253 SSSTSEWFSQRGMDSSSKKVLRWVQRKFMVYNYCKDKKRFSNGLPVEC 296

BLAST of Lsi06G011300 vs. ExPASy TrEMBL
Match: A0A1S3BXV4 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=LOC103494276 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.1e-143
Identity = 250/298 (83.89%), Postives = 273/298 (91.61%), Query Frame = 0

Query: 1   MEIKTSSI-SSSSSKFAIHVLFLCVMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGE 60
           ME  T SI SSSSSK AI+VLF+CV+++FSSSM +SANFLKDIDHTWGGGDRVQILNNGE
Sbjct: 1   MENNTCSISSSSSSKLAIYVLFVCVVLMFSSSMVSSANFLKDIDHTWGGGDRVQILNNGE 60

Query: 61  GLAIALDETSCSGFQSRDQYLFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFL 120
           G+A++LDETSCSGFQSRDQYLFAKIDL++KLVPGNSAGTVTAFY+SS GEFHDEID EFL
Sbjct: 61  GIAVSLDETSCSGFQSRDQYLFAKIDLEMKLVPGNSAGTVTAFYLSSIGEFHDEIDLEFL 120

Query: 121 GRVEEDPYILQTNMFIHGVGKRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIREL 180
           GRVE DPYILQTNMFIHGVG+RE QFFLWFDP+ DFHNYTILWTP HIVL VDGIPIREL
Sbjct: 121 GRVEGDPYILQTNMFIHGVGQREMQFFLWFDPSTDFHNYTILWTPQHIVLLVDGIPIREL 180

Query: 181 KNKKEKGAPFPIGQPMRIYGSLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSD 240
           KNK+EK APFP+ QPMRIYGS+WNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSD
Sbjct: 181 KNKREKRAPFPMVQPMRIYGSIWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSD 240

Query: 241 GRKSNHSNCTTKASTSWLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
             K++ SNC TK+S SWLSS  LDN+S+Q MKWAQT YMFYDYCTD KRFP+GLPLEC
Sbjct: 241 --KNSKSNC-TKSSASWLSSI-LDNKSKQRMKWAQTKYMFYDYCTDTKRFPQGLPLEC 294

BLAST of Lsi06G011300 vs. ExPASy TrEMBL
Match: N0DX83 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth5 PE=3 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 9.1e-138
Identity = 243/298 (81.54%), Postives = 262/298 (87.92%), Query Frame = 0

Query: 1   MEIKTSSISSSSSKFAIHVLFLC-VMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGE 60
           ME  T  +SS SSK AIHV F+C V I+FSSSM +SANFL DI HTWGGGDR+QILNNGE
Sbjct: 1   MENNTCLVSSPSSKLAIHVFFVCAVFIMFSSSMVSSANFLTDIGHTWGGGDRIQILNNGE 60

Query: 61  GLAIALDETSCSGFQSRDQYLFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFL 120
           G+A++LDETSCSGFQSRDQYL+AKIDLQIKLV GNSAGTVTAFY+SS GEFHDE+DFEFL
Sbjct: 61  GIAVSLDETSCSGFQSRDQYLYAKIDLQIKLVSGNSAGTVTAFYLSSIGEFHDEVDFEFL 120

Query: 121 GRVEEDPYILQTNMFIHGVGKRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIREL 180
           GRVE DPYILQTNMFIHGVGKRE QFFLWFDPT DFHNYTILWTP HIV  VDGIPIREL
Sbjct: 121 GRVEGDPYILQTNMFIHGVGKREMQFFLWFDPTDDFHNYTILWTPQHIVQLVDGIPIREL 180

Query: 181 KNKKEKGAPFPIGQPMRIYGSLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSD 240
           KN+K K APFP  QPMRIYGS+WNADSWATRGGAVKIDWTK PFRAWFKNLRVDGCL S 
Sbjct: 181 KNEKGKRAPFPTMQPMRIYGSIWNADSWATRGGAVKIDWTKGPFRAWFKNLRVDGCLRS- 240

Query: 241 GRKSNHSNCTTKASTSWLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
             +++ SNC TK+STSWLSST LDN+S+Q MKWA T YMFYDYCTD KRFPKGLPLEC
Sbjct: 241 -HENSKSNC-TKSSTSWLSST-LDNKSKQRMKWAHTKYMFYDYCTDTKRFPKGLPLEC 294

BLAST of Lsi06G011300 vs. ExPASy TrEMBL
Match: A0A5D3CL19 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00690 PE=3 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 2.3e-136
Identity = 232/271 (85.61%), Postives = 251/271 (92.62%), Query Frame = 0

Query: 27  IFSSSMAASANFLKDIDHTWGGGDRVQILNNGEGLAIALDETSCSGFQSRDQYLFAKIDL 86
           +FSSSM +SANFLKDIDHTWGGGDRVQILNNGEG+A++LDETSCSGFQSRDQYLFAKIDL
Sbjct: 1   MFSSSMVSSANFLKDIDHTWGGGDRVQILNNGEGIAVSLDETSCSGFQSRDQYLFAKIDL 60

Query: 87  QIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLGRVEEDPYILQTNMFIHGVGKRETQFF 146
           ++KLVPGNSAGTVTAFY+SS GEFHDEID EFLGRVE DPYILQTNMFIHGVG+RE QFF
Sbjct: 61  EMKLVPGNSAGTVTAFYLSSIGEFHDEIDLEFLGRVEGDPYILQTNMFIHGVGQREMQFF 120

Query: 147 LWFDPTADFHNYTILWTPIHIVLYVDGIPIRELKNKKEKGAPFPIGQPMRIYGSLWNADS 206
           LWFDP+ DFHNYTILWTP HIVL VDGIPIRELKNK+EK APFP+ QPMRIYGS+WNADS
Sbjct: 121 LWFDPSTDFHNYTILWTPQHIVLLVDGIPIRELKNKREKRAPFPMVQPMRIYGSIWNADS 180

Query: 207 WATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDGRKSNHSNCTTKASTSWLSSTGLDNES 266
           WATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSD  K++ SNC TK+S SWLSS  LDN+S
Sbjct: 181 WATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSD--KNSKSNC-TKSSASWLSSI-LDNKS 240

Query: 267 QQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
           +Q MKWAQT YMFYDYCTD KRFP+GLPLEC
Sbjct: 241 KQRMKWAQTKYMFYDYCTDTKRFPQGLPLEC 267

BLAST of Lsi06G011300 vs. ExPASy TrEMBL
Match: A0A6J1KX23 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111497159 PE=3 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.4e-132
Identity = 235/297 (79.12%), Postives = 259/297 (87.21%), Query Frame = 0

Query: 1   MEIKTSSISSSSSKFAIHVLFLCVMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGEG 60
           ME +T  + S SSKF+ H+LF+C ++IF+S   ASANF K+IDHTW G DRVQIL+NGE 
Sbjct: 1   MEQQTLPV-SCSSKFSTHMLFVC-LVIFASLTPASANFYKNIDHTW-GDDRVQILDNGEV 60

Query: 61  LAIALDETSCSGFQSRDQYLFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLG 120
           + + LD+TS SGFQSR +YLFAKIDLQIKLVPGNSAGTVTAFYVSS GEFHDEIDFEFLG
Sbjct: 61  IRVTLDQTSGSGFQSRGEYLFAKIDLQIKLVPGNSAGTVTAFYVSSTGEFHDEIDFEFLG 120

Query: 121 RVEEDPYILQTNMFIHGVGKRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIRELK 180
           RVE DPYILQTNMF+HGVGKRETQFFLWFDPTADFHNYTILWTP+HIVL VDGIPIRELK
Sbjct: 121 RVEGDPYILQTNMFLHGVGKRETQFFLWFDPTADFHNYTILWTPLHIVLSVDGIPIRELK 180

Query: 181 NKKEKGAPFPIGQPMRIYGSLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDG 240
           N+KEKGAPFP+ QPMRIY SLWNADSWATRGGAVKIDWTKAPFRAW+KNL  DGCLWSDG
Sbjct: 181 NRKEKGAPFPLRQPMRIYASLWNADSWATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDG 240

Query: 241 RKSNHSNCTTKASTSWLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
           +    SNC++  S SWLSST LD  SQ+ MKWAQT YMFYDYCTD KRFP+GLPLEC
Sbjct: 241 K----SNCSS-TSASWLSST-LDRLSQRRMKWAQTKYMFYDYCTDTKRFPQGLPLEC 288

BLAST of Lsi06G011300 vs. ExPASy TrEMBL
Match: A0A6J1F180 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111441218 PE=3 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.3e-131
Identity = 232/288 (80.56%), Postives = 253/288 (87.85%), Query Frame = 0

Query: 10  SSSSKFAIHVLFLCVMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGEGLAIALDETS 69
           S S KF+ H+LF+C ++IF+S   ASANF K+IDHTW G DRVQIL+NGE + + LD+TS
Sbjct: 9   SCSPKFSTHMLFVC-LLIFASLTPASANFYKNIDHTW-GDDRVQILDNGEVIRVTLDQTS 68

Query: 70  CSGFQSRDQYLFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLGRVEEDPYIL 129
            SGFQSR +YLFAKIDLQIKLVPGNSAGTVTAFYVSS GEFHDEIDFEFLGRVE DPYIL
Sbjct: 69  GSGFQSRGEYLFAKIDLQIKLVPGNSAGTVTAFYVSSTGEFHDEIDFEFLGRVEGDPYIL 128

Query: 130 QTNMFIHGVGKRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIRELKNKKEKGAPF 189
           QTNMF+HGVGKRETQFFLWFDPTADFHNYTILWTP HIVL VDGIPIRELKNKKEKGAPF
Sbjct: 129 QTNMFLHGVGKRETQFFLWFDPTADFHNYTILWTPRHIVLSVDGIPIRELKNKKEKGAPF 188

Query: 190 PIGQPMRIYGSLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDGRKSNHSNCT 249
           P+ QPMRIY SLWNADSWATRGGAVKIDWTKAPFRAW+KNL  DGCLWSDG+    SNC+
Sbjct: 189 PLRQPMRIYASLWNADSWATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGK----SNCS 248

Query: 250 TKASTSWLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
           + +S SWLSST LD  SQ+ MKWAQT YMFYDYCTD KRFP+GLPLEC
Sbjct: 249 S-SSASWLSST-LDRVSQRRMKWAQTKYMFYDYCTDTKRFPQGLPLEC 288

BLAST of Lsi06G011300 vs. NCBI nr
Match: XP_038880549.1 (probable xyloglucan endotransglucosylase/hydrolase protein 23 [Benincasa hispida])

HSP 1 Score: 553.1 bits (1424), Expect = 1.4e-153
Identity = 266/297 (89.56%), Postives = 276/297 (92.93%), Query Frame = 0

Query: 1   MEIKTSSISSSSSKFAIHVLFLCVMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGEG 60
           ME +T S+SS SS FAIH+LF+CV+IIFSSSM ASANFLKDIDHTWGGGDRVQILNNGEG
Sbjct: 1   METQTCSVSSCSSNFAIHMLFVCVIIIFSSSMVASANFLKDIDHTWGGGDRVQILNNGEG 60

Query: 61  LAIALDETSCSGFQSRDQYLFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLG 120
           +AIALDETSCSGFQSR+QYLFAKIDLQIKLVPGNSAGTVTAFYVSSK EFHDEIDFEFLG
Sbjct: 61  IAIALDETSCSGFQSRNQYLFAKIDLQIKLVPGNSAGTVTAFYVSSKEEFHDEIDFEFLG 120

Query: 121 RVEEDPYILQTNMFIHGVGKRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIRELK 180
           RVE DPYILQTNMFIHGVGKRETQFFLWFDPT DFHNYTILWTP HIVL VDGIPIRELK
Sbjct: 121 RVEGDPYILQTNMFIHGVGKRETQFFLWFDPTTDFHNYTILWTPFHIVLSVDGIPIRELK 180

Query: 181 NKKEKGAPFPIGQPMRIYGSLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDG 240
           N KEKGAPFP+ QPMRIYGSLWNADSWATRGGAVKIDWTK PFRAWFKNLRVDGCLWSDG
Sbjct: 181 NNKEKGAPFPMRQPMRIYGSLWNADSWATRGGAVKIDWTKGPFRAWFKNLRVDGCLWSDG 240

Query: 241 RKSNHSNCTTKASTSWLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
               +SNC TK+STSWLSST LDNESQQHMKWA TNYMFYDYCTD KRFPKGLPLEC
Sbjct: 241 ----NSNC-TKSSTSWLSST-LDNESQQHMKWAHTNYMFYDYCTDTKRFPKGLPLEC 291

BLAST of Lsi06G011300 vs. NCBI nr
Match: XP_008453617.1 (PREDICTED: xyloglucan endotransglucosylase/hydrolase protein 22-like [Cucumis melo])

HSP 1 Score: 519.2 bits (1336), Expect = 2.3e-143
Identity = 250/298 (83.89%), Postives = 273/298 (91.61%), Query Frame = 0

Query: 1   MEIKTSSI-SSSSSKFAIHVLFLCVMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGE 60
           ME  T SI SSSSSK AI+VLF+CV+++FSSSM +SANFLKDIDHTWGGGDRVQILNNGE
Sbjct: 1   MENNTCSISSSSSSKLAIYVLFVCVVLMFSSSMVSSANFLKDIDHTWGGGDRVQILNNGE 60

Query: 61  GLAIALDETSCSGFQSRDQYLFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFL 120
           G+A++LDETSCSGFQSRDQYLFAKIDL++KLVPGNSAGTVTAFY+SS GEFHDEID EFL
Sbjct: 61  GIAVSLDETSCSGFQSRDQYLFAKIDLEMKLVPGNSAGTVTAFYLSSIGEFHDEIDLEFL 120

Query: 121 GRVEEDPYILQTNMFIHGVGKRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIREL 180
           GRVE DPYILQTNMFIHGVG+RE QFFLWFDP+ DFHNYTILWTP HIVL VDGIPIREL
Sbjct: 121 GRVEGDPYILQTNMFIHGVGQREMQFFLWFDPSTDFHNYTILWTPQHIVLLVDGIPIREL 180

Query: 181 KNKKEKGAPFPIGQPMRIYGSLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSD 240
           KNK+EK APFP+ QPMRIYGS+WNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSD
Sbjct: 181 KNKREKRAPFPMVQPMRIYGSIWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSD 240

Query: 241 GRKSNHSNCTTKASTSWLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
             K++ SNC TK+S SWLSS  LDN+S+Q MKWAQT YMFYDYCTD KRFP+GLPLEC
Sbjct: 241 --KNSKSNC-TKSSASWLSSI-LDNKSKQRMKWAQTKYMFYDYCTDTKRFPQGLPLEC 294

BLAST of Lsi06G011300 vs. NCBI nr
Match: XP_004146359.1 (xyloglucan endotransglucosylase/hydrolase protein 24 isoform X1 [Cucumis sativus] >KGN65442.1 hypothetical protein Csa_020094 [Cucumis sativus] >CCH26405.1 xyloglucan endotransglycosylase [Cucumis sativus])

HSP 1 Score: 499.6 bits (1285), Expect = 1.9e-137
Identity = 243/298 (81.54%), Postives = 262/298 (87.92%), Query Frame = 0

Query: 1   MEIKTSSISSSSSKFAIHVLFLC-VMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGE 60
           ME  T  +SS SSK AIHV F+C V I+FSSSM +SANFL DI HTWGGGDR+QILNNGE
Sbjct: 1   MENNTCLVSSPSSKLAIHVFFVCAVFIMFSSSMVSSANFLTDIGHTWGGGDRIQILNNGE 60

Query: 61  GLAIALDETSCSGFQSRDQYLFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFL 120
           G+A++LDETSCSGFQSRDQYL+AKIDLQIKLV GNSAGTVTAFY+SS GEFHDE+DFEFL
Sbjct: 61  GIAVSLDETSCSGFQSRDQYLYAKIDLQIKLVSGNSAGTVTAFYLSSIGEFHDEVDFEFL 120

Query: 121 GRVEEDPYILQTNMFIHGVGKRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIREL 180
           GRVE DPYILQTNMFIHGVGKRE QFFLWFDPT DFHNYTILWTP HIV  VDGIPIREL
Sbjct: 121 GRVEGDPYILQTNMFIHGVGKREMQFFLWFDPTDDFHNYTILWTPQHIVQLVDGIPIREL 180

Query: 181 KNKKEKGAPFPIGQPMRIYGSLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSD 240
           KN+K K APFP  QPMRIYGS+WNADSWATRGGAVKIDWTK PFRAWFKNLRVDGCL S 
Sbjct: 181 KNEKGKRAPFPTMQPMRIYGSIWNADSWATRGGAVKIDWTKGPFRAWFKNLRVDGCLRS- 240

Query: 241 GRKSNHSNCTTKASTSWLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
             +++ SNC TK+STSWLSST LDN+S+Q MKWA T YMFYDYCTD KRFPKGLPLEC
Sbjct: 241 -HENSKSNC-TKSSTSWLSST-LDNKSKQRMKWAHTKYMFYDYCTDTKRFPKGLPLEC 294

BLAST of Lsi06G011300 vs. NCBI nr
Match: TYK11848.1 (xyloglucan endotransglucosylase/hydrolase protein 22-like [Cucumis melo var. makuwa])

HSP 1 Score: 495.0 bits (1273), Expect = 4.6e-136
Identity = 232/271 (85.61%), Postives = 251/271 (92.62%), Query Frame = 0

Query: 27  IFSSSMAASANFLKDIDHTWGGGDRVQILNNGEGLAIALDETSCSGFQSRDQYLFAKIDL 86
           +FSSSM +SANFLKDIDHTWGGGDRVQILNNGEG+A++LDETSCSGFQSRDQYLFAKIDL
Sbjct: 1   MFSSSMVSSANFLKDIDHTWGGGDRVQILNNGEGIAVSLDETSCSGFQSRDQYLFAKIDL 60

Query: 87  QIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLGRVEEDPYILQTNMFIHGVGKRETQFF 146
           ++KLVPGNSAGTVTAFY+SS GEFHDEID EFLGRVE DPYILQTNMFIHGVG+RE QFF
Sbjct: 61  EMKLVPGNSAGTVTAFYLSSIGEFHDEIDLEFLGRVEGDPYILQTNMFIHGVGQREMQFF 120

Query: 147 LWFDPTADFHNYTILWTPIHIVLYVDGIPIRELKNKKEKGAPFPIGQPMRIYGSLWNADS 206
           LWFDP+ DFHNYTILWTP HIVL VDGIPIRELKNK+EK APFP+ QPMRIYGS+WNADS
Sbjct: 121 LWFDPSTDFHNYTILWTPQHIVLLVDGIPIRELKNKREKRAPFPMVQPMRIYGSIWNADS 180

Query: 207 WATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDGRKSNHSNCTTKASTSWLSSTGLDNES 266
           WATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSD  K++ SNC TK+S SWLSS  LDN+S
Sbjct: 181 WATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSD--KNSKSNC-TKSSASWLSSI-LDNKS 240

Query: 267 QQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
           +Q MKWAQT YMFYDYCTD KRFP+GLPLEC
Sbjct: 241 KQRMKWAQTKYMFYDYCTDTKRFPQGLPLEC 267

BLAST of Lsi06G011300 vs. NCBI nr
Match: XP_023003613.1 (xyloglucan endotransglucosylase/hydrolase protein 22-like [Cucurbita maxima])

HSP 1 Score: 481.1 bits (1237), Expect = 6.9e-132
Identity = 235/297 (79.12%), Postives = 259/297 (87.21%), Query Frame = 0

Query: 1   MEIKTSSISSSSSKFAIHVLFLCVMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGEG 60
           ME +T  + S SSKF+ H+LF+C ++IF+S   ASANF K+IDHTW G DRVQIL+NGE 
Sbjct: 1   MEQQTLPV-SCSSKFSTHMLFVC-LVIFASLTPASANFYKNIDHTW-GDDRVQILDNGEV 60

Query: 61  LAIALDETSCSGFQSRDQYLFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLG 120
           + + LD+TS SGFQSR +YLFAKIDLQIKLVPGNSAGTVTAFYVSS GEFHDEIDFEFLG
Sbjct: 61  IRVTLDQTSGSGFQSRGEYLFAKIDLQIKLVPGNSAGTVTAFYVSSTGEFHDEIDFEFLG 120

Query: 121 RVEEDPYILQTNMFIHGVGKRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIRELK 180
           RVE DPYILQTNMF+HGVGKRETQFFLWFDPTADFHNYTILWTP+HIVL VDGIPIRELK
Sbjct: 121 RVEGDPYILQTNMFLHGVGKRETQFFLWFDPTADFHNYTILWTPLHIVLSVDGIPIRELK 180

Query: 181 NKKEKGAPFPIGQPMRIYGSLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDG 240
           N+KEKGAPFP+ QPMRIY SLWNADSWATRGGAVKIDWTKAPFRAW+KNL  DGCLWSDG
Sbjct: 181 NRKEKGAPFPLRQPMRIYASLWNADSWATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDG 240

Query: 241 RKSNHSNCTTKASTSWLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
           +    SNC++  S SWLSST LD  SQ+ MKWAQT YMFYDYCTD KRFP+GLPLEC
Sbjct: 241 K----SNCSS-TSASWLSST-LDRLSQRRMKWAQTKYMFYDYCTDTKRFPQGLPLEC 288

BLAST of Lsi06G011300 vs. TAIR 10
Match: AT5G57560.1 (Xyloglucan endotransglucosylase/hydrolase family protein )

HSP 1 Score: 328.2 bits (840), Expect = 7.0e-90
Identity = 159/283 (56.18%), Postives = 204/283 (72.08%), Query Frame = 0

Query: 18  HVLFLCVMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGEGLAIALDETSCSGFQSRD 77
           ++L L + +I +SS+  SANF +D++ TWG G R QI NNGE L ++LD++S SGFQS++
Sbjct: 5   YLLPLFLSLIITSSV--SANFQRDVEITWGDG-RGQIKNNGELLTLSLDKSSGSGFQSKN 64

Query: 78  QYLFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLGRVEEDPYILQTNMFIHG 137
           +YLF K+ +Q+KLVPGNSAGTVT  Y+ S G   DEIDFEFLG    +PY L TN++  G
Sbjct: 65  EYLFGKVSMQMKLVPGNSAGTVTTLYLKSPGTTWDEIDFEFLGNSSGEPYTLHTNVYTQG 124

Query: 138 VGKRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIRELKNKKEKGAPFPIGQPMRI 197
            G +E QF LWFDPTA+FH YTILW P  I+  VDG PIRE KN +  G  FP  +PMR+
Sbjct: 125 KGDKEQQFKLWFDPTANFHTYTILWNPQRIIFTVDGTPIREFKNMESLGTLFPKNKPMRM 184

Query: 198 YGSLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDGRKSNHSNCTTKAST--S 257
           Y SLWNAD WATRGG VK DW+KAPF A ++  + + C+WS+G KS+  N + + +T  S
Sbjct: 185 YSSLWNADDWATRGGLVKTDWSKAPFTASYRGFQQEACVWSNG-KSSCPNASKQGTTTGS 244

Query: 258 WLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLECL 299
           WLS   LD+ +QQ M+W Q NYM Y+YCTD KRFP+GLP ECL
Sbjct: 245 WLSQE-LDSTAQQRMRWVQRNYMIYNYCTDAKRFPQGLPKECL 282

BLAST of Lsi06G011300 vs. TAIR 10
Match: AT4G25810.1 (xyloglucan endotransglycosylase 6 )

HSP 1 Score: 324.7 bits (831), Expect = 7.8e-89
Identity = 155/277 (55.96%), Postives = 196/277 (70.76%), Query Frame = 0

Query: 24  VMIIFSSSM--AASANFLKDIDHTWGGGDRVQILNNGEGLAIALDETSCSGFQSRDQYLF 83
           V+ + +S M  + SANF +D++ TWG G R QI NNG+ L ++LD+ S SGFQS+++YLF
Sbjct: 10  VVALLASFMICSVSANFQRDVEITWGDG-RGQITNNGDLLTLSLDKASGSGFQSKNEYLF 69

Query: 84  AKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLGRVEEDPYILQTNMFIHGVGKR 143
            KID+QIKLV GNSAGTVTA+Y+ S G   DEIDFEFLG +  DPY L TN+F  G G R
Sbjct: 70  GKIDMQIKLVAGNSAGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKGDR 129

Query: 144 ETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIRELKNKKEKGAPFPIGQPMRIYGSL 203
           E QF LWFDPT+DFH Y+ILW P  I+  VDG PIRE KN + +G  FP  QPMR+Y SL
Sbjct: 130 EQQFKLWFDPTSDFHTYSILWNPQRIIFSVDGTPIREFKNMESQGTLFPKNQPMRMYSSL 189

Query: 204 WNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDGRKSNHSNCTTKASTSWLSSTG 263
           WNA+ WATRGG VK DW+KAPF A ++    + C+  +G+ S  +     ++ SWLS   
Sbjct: 190 WNAEEWATRGGLVKTDWSKAPFTASYRGFNEEACVVINGQSSCPNVSGQGSTGSWLSQE- 249

Query: 264 LDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLECL 299
           LD+  Q+ M+W Q NYM Y+YCTD KRFP+GLP ECL
Sbjct: 250 LDSTGQEQMRWVQNNYMIYNYCTDAKRFPQGLPRECL 284

BLAST of Lsi06G011300 vs. TAIR 10
Match: AT5G57550.1 (xyloglucan endotransglucosylase/hydrolase 25 )

HSP 1 Score: 312.4 bits (799), Expect = 4.0e-85
Identity = 155/286 (54.20%), Postives = 191/286 (66.78%), Query Frame = 0

Query: 13  SKFAIHVLF-LCVMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGEGLAIALDETSCS 72
           S F + +LF L V      S   +  F  + D TWG G R ++LNNGE L ++LD  S S
Sbjct: 4   STFILSLLFTLTVSTTTLFSPVFAGTFDTEFDITWGDG-RGKVLNNGELLTLSLDRASGS 63

Query: 73  GFQSRDQYLFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLGRVEEDPYILQT 132
           GFQ++ +YLF KID+Q+KLVPGNSAGTVTA+Y+ SKG+  DEIDFEFLG +  DPY + T
Sbjct: 64  GFQTKKEYLFGKIDMQLKLVPGNSAGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHT 123

Query: 133 NMFIHGVGKRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIRELKNKKEKGAPFPI 192
           N++  G G RE QF LWFDPTADFH Y++LW P HIV  VD IP+RE KN +  G  +P 
Sbjct: 124 NVYTQGKGDREQQFHLWFDPTADFHTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPK 183

Query: 193 GQPMRIYGSLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDGRKSNHSNCTTK 252
            QPMR+Y SLWNAD WATRGG VK DW+KAPF A ++N R D C+ S GR    S+C   
Sbjct: 184 LQPMRLYSSLWNADQWATRGGLVKTDWSKAPFTASYRNFRADACVSSGGR----SSCPA- 243

Query: 253 ASTSWLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
            S  W S   LD  ++  M+  Q  YM Y+YCTD KRFP+G P EC
Sbjct: 244 GSPRWFSQR-LDLTAEDKMRVVQRKYMIYNYCTDTKRFPQGFPKEC 282

BLAST of Lsi06G011300 vs. TAIR 10
Match: AT2G18800.1 (xyloglucan endotransglucosylase/hydrolase 21 )

HSP 1 Score: 310.8 bits (795), Expect = 1.2e-84
Identity = 154/288 (53.47%), Postives = 197/288 (68.40%), Query Frame = 0

Query: 20  LFLCVMIIFSSSMAASANFLKDIDHTWGGGDRVQILNNGEGLAIALDETSCSGFQSRDQY 79
           LFL + I+    +    +F +DID TWG G R  ILNNG  L + LD++S SGFQS+ +Y
Sbjct: 13  LFLGLSILL---VVHGKDFNQDIDITWGDG-RGNILNNGTLLNLGLDQSSGSGFQSKAEY 72

Query: 80  LFAKIDLQIKLVPGNSAGTVTAFYVSSKGEFHDEIDFEFLGRVEEDPYILQTNMFIHGVG 139
           L+ K+D+QIKLVPGNSAGTVT FY+ S+G   DEIDFEFLG V  DPYI+ TN++  G G
Sbjct: 73  LYGKVDMQIKLVPGNSAGTVTTFYLKSQGLTWDEIDFEFLGNVSGDPYIVHTNVYTQGKG 132

Query: 140 KRETQFFLWFDPTADFHNYTILWTPIHIVLYVDGIPIRELKNKKEKGAPFPIGQPMRIYG 199
            RE QF+LWFDPTA FHNY+ILW P HIV Y+DG PIRE KN +  G  +P  QPMR+YG
Sbjct: 133 DREQQFYLWFDPTAAFHNYSILWNPSHIVFYIDGKPIREFKNLEVLGVAYPKNQPMRMYG 192

Query: 200 SLWNADSWATRGGAVKIDWTKAPFRAWFKNLRVD-GCLWS--DGRKS------NHSNCTT 259
           SLWNAD WATRGG VK +W++ PF A F N   +  C+WS  +G  +        S  ++
Sbjct: 193 SLWNADDWATRGGLVKTNWSQGPFVASFMNYNSENACVWSIVNGTTTTSPCSPGDSTSSS 252

Query: 260 KASTS-WLSSTGLDNESQQHMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
            +STS W S  G+D+ S++ ++W Q  +M Y+YC D KRF  GLP+EC
Sbjct: 253 SSSTSEWFSQRGMDSSSKKVLRWVQRKFMVYNYCKDKKRFSNGLPVEC 296

BLAST of Lsi06G011300 vs. TAIR 10
Match: AT5G57530.1 (xyloglucan endotransglucosylase/hydrolase 12 )

HSP 1 Score: 308.9 bits (790), Expect = 4.4e-84
Identity = 148/269 (55.02%), Postives = 183/269 (68.03%), Query Frame = 0

Query: 34  ASANFLKDIDHTWGGGDRVQILNNGEGLAIALDETSCSGFQSRDQYLFAKIDLQIKLVPG 93
           A+ +F    D TWG G R  I  +G+ L   LD+TS SGFQS+ +YLF KID++IKLVPG
Sbjct: 23  ATGSFYDSFDITWGAG-RANIFESGQLLTCTLDKTSGSGFQSKKEYLFGKIDMKIKLVPG 82

Query: 94  NSAGTVTAFYVSSKGEFHDEIDFEFLGRVEEDPYILQTNMFIHGVGKRETQFFLWFDPTA 153
           NSAGTVTA+Y+SSKGE  DEIDFEFLG V   PY++ TN+F  G G RE QF+LWFDPTA
Sbjct: 83  NSAGTVTAYYLSSKGETWDEIDFEFLGNVTGQPYVIHTNVFTGGKGNREMQFYLWFDPTA 142

Query: 154 DFHNYTILWTPIHIVLYVDGIPIRELKNKKEKGAPFPIGQPMRIYGSLWNADSWATRGGA 213
           DFH YT+LW P++I+  VDGIPIR  KN +  G  +P  QPM+IY SLW AD WAT+GG 
Sbjct: 143 DFHTYTVLWNPLNIIFLVDGIPIRVFKNNEANGVAYPKSQPMKIYSSLWEADDWATQGGK 202

Query: 214 VKIDWTKAPFRAWFKNLR-VDGC----LWSDGRKSNHSNCTTKASTSWLSSTGLDNESQQ 273
           VK DWT APF A +++   VD C    +W      N   C    S SW+ +T L++    
Sbjct: 203 VKTDWTNAPFSASYRSFNDVDCCSRTSIW------NWVTCNAN-SNSWMWTT-LNSNQLG 262

Query: 274 HMKWAQTNYMFYDYCTDIKRFPKGLPLEC 298
            +KW Q +YM Y+YCTD KRFP+GLP EC
Sbjct: 263 QLKWVQKDYMIYNYCTDFKRFPQGLPTEC 282

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q388579.9e-8956.18Xyloglucan endotransglucosylase/hydrolase protein 22 OS=Arabidopsis thaliana OX=... [more]
Q389101.1e-8755.96Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis tha... [more]
Q389075.6e-8454.20Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis tha... [more]
P356947.3e-8451.39Xyloglucan endotransglucosylase/hydrolase 2 OS=Glycine max OX=3847 PE=2 SV=1[more]
Q9ZV401.6e-8353.47Probable xyloglucan endotransglucosylase/hydrolase protein 21 OS=Arabidopsis tha... [more]
Match NameE-valueIdentityDescription
A0A1S3BXV41.1e-14383.89Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=LOC10349427... [more]
N0DX839.1e-13881.54Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth5 PE=... [more]
A0A5D3CL192.3e-13685.61Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A6J1KX233.4e-13279.12Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1F1801.3e-13180.56Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_038880549.11.4e-15389.56probable xyloglucan endotransglucosylase/hydrolase protein 23 [Benincasa hispida... [more]
XP_008453617.12.3e-14383.89PREDICTED: xyloglucan endotransglucosylase/hydrolase protein 22-like [Cucumis me... [more]
XP_004146359.11.9e-13781.54xyloglucan endotransglucosylase/hydrolase protein 24 isoform X1 [Cucumis sativus... [more]
TYK11848.14.6e-13685.61xyloglucan endotransglucosylase/hydrolase protein 22-like [Cucumis melo var. mak... [more]
XP_023003613.16.9e-13279.12xyloglucan endotransglucosylase/hydrolase protein 22-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G57560.17.0e-9056.18Xyloglucan endotransglucosylase/hydrolase family protein [more]
AT4G25810.17.8e-8955.96xyloglucan endotransglycosylase 6 [more]
AT5G57550.14.0e-8554.20xyloglucan endotransglucosylase/hydrolase 25 [more]
AT2G18800.11.2e-8453.47xyloglucan endotransglucosylase/hydrolase 21 [more]
AT5G57530.14.4e-8455.02xyloglucan endotransglucosylase/hydrolase 12 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.60.120.200coord: 23..299
e-value: 1.2E-93
score: 315.4
NoneNo IPR availablePANTHERPTHR31062:SF153XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 21-RELATEDcoord: 14..297
IPR016455Xyloglucan endotransglucosylase/hydrolasePIRSFPIRSF005604EndGlu_transfcoord: 3..299
e-value: 1.6E-110
score: 366.8
IPR016455Xyloglucan endotransglucosylase/hydrolaseCDDcd02176GH16_XETcoord: 33..297
e-value: 1.09357E-135
score: 382.703
IPR010713Xyloglucan endo-transglycosylase, C-terminalPFAMPF06955XET_Ccoord: 252..297
e-value: 1.2E-18
score: 66.7
IPR000757Glycoside hydrolase family 16PFAMPF00722Glyco_hydro_16coord: 49..220
e-value: 4.2E-55
score: 186.0
IPR000757Glycoside hydrolase family 16PROSITEPS51762GH16_2coord: 4..227
score: 25.890564
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 14..297
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 42..298

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G011300.1Lsi06G011300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042546 cell wall biogenesis
biological_process GO:0071555 cell wall organization
biological_process GO:0010411 xyloglucan metabolic process
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006073 cellular glucan metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005618 cell wall
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity