Lsi06G010700 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G010700
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionprotein OS-9 homolog
Locationchr06: 20925074 .. 20930485 (-)
RNA-Seq ExpressionLsi06G010700
SyntenyLsi06G010700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAATATTCATTGGTCCAGCATGCGTGGAAGTGCATCACTCTCTATCGTCAACACAGGCCGTAAAACCGAAGACCCACTTCCTTCTTCCTCTCGCCGTCGTCGTCGCCGTCGTCGCCCTTCCGCCCCCAGCTGAAAATACCTAACCCTTCCACTTTGCTCCCTTCCTTTTCACCATTGTACGTTTGTTTCAGGACGCAGAAATGACGAAATTGATGTGGTTGATCCTTTTAGCTGGTAATCTTTGCCACTTTGCGTTAGCTGATCAGATCTTCCCAGCTCAATTAGGTGAAATTCTTCTCCCTCATTTTCTTTTTCTTGAACTTTTCACATGAAAACTTGAGAAATAGACGTGATTGATGAATTTATATTGTGGGTAGTTCCTTGCGTAATATATTTCATCGCATATTGGAATTAGCGAACTCCAGAGGGTGGTATTTCATAGGTTGTTTAACACGACTTGAAACTTGAAACATTTATTACTTTTGCTTATTATGACAGTAACTTGTTAGTATGAAATTTTATTCTCTCTTCTTTCTGATTGTTGATTAGCAGGTGGTACATTTAGTCGCAACTCTCGTGGGCCAAAATACAATATAGAATTCCATCCACAAGACTCGCCTTACAATCCTGTAAGCTTTTTAATATACTTAGTTGTTAATTCTTGATATTAATAGGACCTTGTTTGCTTATATGATGTAATAATATGGGTAGATTTTGGGTCTTAGGCATCCCTCCCGCTTTCTTGTTAACTCGGAGTGTACTGTAACTTTGTTCCAAATATCCTCAATTAAGTAACCCAGTATATAGTACTGTACATCTTTACGAAGTCAAAGATTTTAGATTCTGGTATCTTCATATATCAGCATCTCGATAAGTAGTACTGCATCTTCAGAATTGTAACCTTCCCATGACATATAAGCTACTAATACGTTTTTTCTCAAAGCAAGTTTGCCACCAACTATAGCAGCACCTAAAATTTGCCATTTTTTAATGTATTTACCCTGGTTCACTTGGGACTTTTGCTGCATACAAGGTTTTTTTTTGTTAGAAAGTTGATACATAACTAATGAAACTCTTCAAGTATACCCATTACCCAAAAAAATGATCTTGTCAATATTGGTATAAATGATCTTTCCCCCGTGTTTGGGTATTCGGGTATAGCGGCAACCCCTGAATCCTGAGCCACTTGGCGTTCCAACCTAGTTCCAACAATGCACTTTTTGGACCAAGAAAGCGAGACTGCTTGACGTTGCATATTAGAATTCATTAAAACATGATTTGCATCCTTATGTTCGATAAAAGGAATGAGGGAAGCTCCAATAGAAAAATATTGGAAGGGAAAAAGATTTCGAAGATGAACTTGTTCCCATTCAATAGTCCAGAATTATTGACGGTATCGAGTTGGAACAATCCGTTCTGCATTTCTGCACACGTTCATTAATTAATCTCTAGTTATCTTATTTTGCTGTTTCTTTGTTTGAGTTTATCATCGTATGTTTTCTCTGTTGGCCTAATTTTTTAGTTTTATACAATAGATTGTTTCCCACTCTGTGAAATATATTCATTAGTTGCTTTCTTTCTCTGAGACAGAGGATTTGAGGAAGGGCATTGGAGATTTTGGCATTGGAGATTGATAGTTGATTGTTTTGTTTGGCACGACTAAATACAATAAATGAATGACGAGAAATTTTCTGTGTTACATTGATCCATGGCACCAAATTTTGAAGCAAATTAAAATTGTAGTTTCATCCTTTTGGTATTAAGCAGTAGGAATATTTGGTATGCAAATGATTTCTTGAACTCTAGGACCCTCTTAGATGGCCTCTACATGAGACTGGTCGTTAAGATGCACTATGGAAGAGGAGTTGGGAAGATGGCTTCTATATGCACTGTATCGCTTAGGCTTGATAATTAAGTCATTGTATGTACTAAATTCTGATGCAAATGTAAGTGCAAATTTAAAACACCTTCTCCGTGCATGAAGTTTTATCGGTAATGTTTTCCATCAACTTGAAGAGTTGAGATATTGAGCCTGTAATCTGTACCCGTACCCAGGTGTGTGGGAAAGTCACATTTCCATTTACAATCCCATTTGCCTTACAAGTTACCGTATTCTTTTCCTTATTGTAGACTTCCTACAGAATGTTTGTTCCCTAAGCTGTATGATATCTGTCTTCAGAATTCAAGAATATGTAGGTATGTCGTATGCCATCTGAACTTACTATGTTTTTGTGATTTTGCAGGACGATGATCAGGAATCTGTGTTTATGCCCAATAAAAATGGAAAGAATTACTTATGTTACTTGCCTAAGGTGGAGAAGTCCAAGAGCGGAAAGCCAACTGTTCAAGTGAACATGAGTAGCATGATTGTGGAATCTGAGAAGCGAGTCAAATTGAAGACTCCAGATGAGCTGCTTGAGGCATTAAAAGAGCAATGCTTTGTCCGGGTTAGGATCTATCGCTGAATATTGTCTGATGACTATATTATCTACATTTTAGAATCATATCTCAAAAGAAAAGTAAAGCTGTAAGCTGTGTTTTTTAATGTGTGCATTGAGAAATTGGCATCCCAATAAAAAGTTGAGGCCCATTCGATAACCATTTTATTTTTAATTTTTGGTTTTTGAAAATTAAGCTTCTAAAAACTACTTCCACCCATTACTATTAGTAGTTTTGAAAATTAAAAAATGTAGTTTTTAAACCCCTCTTTTTTTTTTTTTGGAATTCGAATTTGACTAAGAATCCAAATTCTTCCTTAAGAAAGATGACAACCATGCTATAGAAATTGTGAGCAAACAAGCTTAATTTTCTCTATGGTGGCCACCTACCTAGGATTTAATATCCTTCTGGTCTCTTTGACACCCAAATATTGTAGGGTCAAGCGGGTTGTCTCGAGAGATTAGTTGAGGTGCACATAATTTCAAAAACAAAAAACTAGATGGTTATCAAATAAGGCTTAAAGAAAGGTTAATTTTATTTCAAACGATGCCTAAAGAAATTAAGACATTTTCTTCTGTAATTTTTAAATAATACATATGTATTAAAAGTAAACAATGTGTTTAAAAAATGCAAGTGAAGTGTGTGCTTTGGGGTTGCCTTGCCTCATGTGAGACAAGGTGCCTTTCTTGCGCTCAACACCAATAAAGCTATTAAATACTAAGGTCTTTTGTTGTAAAATATATGCGTTTTTAAGTAAAACATGTGATGATTATGTCCTGAACAATTGACATGTTGACCTTGCATCATCTACTTCATTTTGCAGCAAGAGGGTTGGTGGACATATGAATTTTGTTACCAGAAAACACTAAAACAATTTCATTTGGAGGATGAAAAGGTAGTCTCGTATAGTGCTTTATAATCCATTCTATATCTGATTCTCTAATCTTAGCTTATGAATAAGGAAGGCAACTGTCATTGTTTATTGGTTAATTAAAAACGTAAAATCATGCTGTGTTAAGTAGCAAAGACTTTTTTGACGTTTCTGTGTTTTGTCTTACAGGTAGTTCAGGAATTTGTATTGGGCGTCTATGATGCAGAGGCAACTGCTAATCTCAATGAGAATCTCTCTGATATATCTACTTTGAAGGATCCTCGCTCAAAAGATGCATCCCAAAGGTGTGCTTTAATTTTCATTAGTTACGAGAAAAGATATTATGAACAGGCTTTAAGTAATAGGAAAAGGCAATCTTCAAGCAGTAAAGCTTGTAATTTTCCTAGTTCTATCACTTCTCAGAAGTAGTAAGTTGGAGGCATACATGAAAAAGCAAAAACTTGTATGAGTTTTCATCCGGCGTTCTACTATTAATTTGTCTGTGTTTCTTCCAGTAGTCAATTCTTACATGTGCCAAATATGACACTCAAGACATGAAACAGAAAGCTAGTATGAATTTAGTGTTTGAAAGGTTTGATTGATCATATTAGTAGAACTCAAGGATACAGTTGTACAACTTATAGAAGAGATCCTGTTTAAATAAGATCGTAATGATTGATCATACAAGTGTGTTGAACAGTGCAAGAGTAAGATTAGTGCTTTGTTGAGTTATGTCATACACAAGAATTGTACGATAGATATAATAAGATCTATTATTTGTTAATAGACAACTAATCCCAATTTCTCAAAATAATGACCGATCTACATGACTCTCTCAGGTATCATGCTCATCATTACACAAATGGAACCATGTGTGATCTCACAAATCAACCGCGAGAAACTGAGGTACAAATAATTAATGATTTACTTTGTCACCCTTTTTTCTTTGAAAAATACTTAGGTGCATCCCGTGCAGCATCTATCTTTGTGCATTATCAACAAATTGTCCAATTATAATTTGTTGTGACAAATTCTTCTTATTTCTTTTTAAAATTATATAGATTGAGTGCATCCATCCAAGTATTACTATTTTTTTTTCCATTACTGATGTTGATTCTTAATTACATTTCAGGTTAGATTTGTTTGCTCGGAGCCCAGGGCCATGATTAATTCTATCACAGAACTATCAACATGCAAGTATGCACTTACAGTGCGATGCCCGACGCTTTGCAAGCATCCGTAAATCTCTCGCTCTCTTTTCTCTATTACCTACACAAACACATCCAGTTTTTCTGACTGTTAGAGGAAATATAACGACGAAATTAAGTGTTACTGGCAAGCATAAGTTTGTGACGGGGAGTTGAGAAAAGGAGAACTCTGCCCGTATCAACCGAATCACTTTAAAGAGCAACCTGCTGTCAGCTAAATTATGTAGCTCTATAATCGTGTAGCTCTACAATTGTGTACAAGCCAAACCAATAGGAGATAGGAAACTAGTATAGGAATTTAAAAGAATCGAAAGTTGACCCAACTTAAAAGAGTTCACATCAAGATGTCAATTTTATTGATAGATACAGTTTTTCGTTTTCGTTTCTCTTATTTATTATGACTGTGCAGGTTATTCCAGGAAGAGAGACCAGTGTGGTACATCATTAACTGCAACGTGCTCCCCGATGATTACAAGGAAACAAAGCGAGTCGACGAATTAGCAGATGAGATTGTCATGGTTACAGACGTCAAATATCCAAAAAATGAATCTGAAGATTAATGTGAATATTTATATCATATAAGTTTTATCAACCAAACTAACCTTACAAAAGAAGCTCTTCCTCTGTAGATTTTTTCTCAAATTCAAATTATACAATTGCCGTGAAGTATCCATCGATTTTCAAGCCCCACGACGTGGAATTTCGTATGTTTTGATCATATTTGAATATATAGGTCAACCTGAAAGCTAGTCATTTTATGGGATTTATTGTTTTTGACAATGATTCAACTTATTGTAGAGGAAAAGTAATGAGAGTTTGAATGGGTAAACTCATAGCTTGCTTTTATTGAGACATTTACATCACTACCACAATAATAAACAATTGAAAGACCA

mRNA sequence

CAAATATTCATTGGTCCAGCATGCGTGGAAGTGCATCACTCTCTATCGTCAACACAGGCCGTAAAACCGAAGACCCACTTCCTTCTTCCTCTCGCCGTCGTCGTCGCCGTCGTCGCCCTTCCGCCCCCAGCTGAAAATACCTAACCCTTCCACTTTGCTCCCTTCCTTTTCACCATTGTACGTTTGTTTCAGGACGCAGAAATGACGAAATTGATGTGGTTGATCCTTTTAGCTGGTAATCTTTGCCACTTTGCGTTAGCTGATCAGATCTTCCCAGCTCAATTAGGTGGTACATTTAGTCGCAACTCTCGTGGGCCAAAATACAATATAGAATTCCATCCACAAGACTCGCCTTACAATCCTGACGATGATCAGGAATCTGTGTTTATGCCCAATAAAAATGGAAAGAATTACTTATGTTACTTGCCTAAGGTGGAGAAGTCCAAGAGCGGAAAGCCAACTGTTCAAGTGAACATGAGTAGCATGATTGTGGAATCTGAGAAGCGAGTCAAATTGAAGACTCCAGATGAGCTGCTTGAGGCATTAAAAGAGCAATGCTTTGTCCGGCAAGAGGGTTGGTGGACATATGAATTTTGTTACCAGAAAACACTAAAACAATTTCATTTGGAGGATGAAAAGGTAGTAGTTCAGGAATTTGTATTGGGCGTCTATGATGCAGAGGCAACTGCTAATCTCAATGAGAATCTCTCTGATATATCTACTTTGAAGGATCCTCGCTCAAAAGATGCATCCCAAAGAAGTAGTAAGTTGGAGGCATACATGAAAAAGCAAAAACTTGTTAGATTTGTTTGCTCGGAGCCCAGGGCCATGATTAATTCTATCACAGAACTATCAACATGCAAGTATGCACTTACAGTGCGATGCCCGACGCTTTGCAAGCATCCGTTATTCCAGGAAGAGAGACCAGTGTGGTACATCATTAACTGCAACGTGCTCCCCGATGATTACAAGGAAACAAAGCGAGTCGACGAATTAGCAGATGAGATTGTCATGGTTACAGACGTCAAATATCCAAAAAATGAATCTGAAGATTAATGTGAATATTTATATCATATAAGTTTTATCAACCAAACTAACCTTACAAAAGAAGCTCTTCCTCTGTAGATTTTTTCTCAAATTCAAATTATACAATTGCCGTGAAGTATCCATCGATTTTCAAGCCCCACGACGTGGAATTTCGTATGTTTTGATCATATTTGAATATATAGGTCAACCTGAAAGCTAGTCATTTTATGGGATTTATTGTTTTTGACAATGATTCAACTTATTGTAGAGGAAAAGTAATGAGAGTTTGAATGGGTAAACTCATAGCTTGCTTTTATTGAGACATTTACATCACTACCACAATAATAAACAATTGAAAGACCA

Coding sequence (CDS)

ATGACGAAATTGATGTGGTTGATCCTTTTAGCTGGTAATCTTTGCCACTTTGCGTTAGCTGATCAGATCTTCCCAGCTCAATTAGGTGGTACATTTAGTCGCAACTCTCGTGGGCCAAAATACAATATAGAATTCCATCCACAAGACTCGCCTTACAATCCTGACGATGATCAGGAATCTGTGTTTATGCCCAATAAAAATGGAAAGAATTACTTATGTTACTTGCCTAAGGTGGAGAAGTCCAAGAGCGGAAAGCCAACTGTTCAAGTGAACATGAGTAGCATGATTGTGGAATCTGAGAAGCGAGTCAAATTGAAGACTCCAGATGAGCTGCTTGAGGCATTAAAAGAGCAATGCTTTGTCCGGCAAGAGGGTTGGTGGACATATGAATTTTGTTACCAGAAAACACTAAAACAATTTCATTTGGAGGATGAAAAGGTAGTAGTTCAGGAATTTGTATTGGGCGTCTATGATGCAGAGGCAACTGCTAATCTCAATGAGAATCTCTCTGATATATCTACTTTGAAGGATCCTCGCTCAAAAGATGCATCCCAAAGAAGTAGTAAGTTGGAGGCATACATGAAAAAGCAAAAACTTGTTAGATTTGTTTGCTCGGAGCCCAGGGCCATGATTAATTCTATCACAGAACTATCAACATGCAAGTATGCACTTACAGTGCGATGCCCGACGCTTTGCAAGCATCCGTTATTCCAGGAAGAGAGACCAGTGTGGTACATCATTAACTGCAACGTGCTCCCCGATGATTACAAGGAAACAAAGCGAGTCGACGAATTAGCAGATGAGATTGTCATGGTTACAGACGTCAAATATCCAAAAAATGAATCTGAAGATTAA

Protein sequence

MTKLMWLILLAGNLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHPQDSPYNPDDDQESVFMPNKNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTPDELLEALKEQCFVRQEGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNENLSDISTLKDPRSKDASQRSSKLEAYMKKQKLVRFVCSEPRAMINSITELSTCKYALTVRCPTLCKHPLFQEERPVWYIINCNVLPDDYKETKRVDELADEIVMVTDVKYPKNESED
Homology
BLAST of Lsi06G010700 vs. ExPASy Swiss-Prot
Match: Q8GWH3 (Protein OS-9 homolog OS=Arabidopsis thaliana OX=3702 GN=OS9 PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.1e-73
Identity = 144/273 (52.75%), Postives = 192/273 (70.33%), Query Frame = 0

Query: 4   LMWLILLAGNLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHPQDSPYNPDDDQESVFM 63
           L+ L+++A +      +DQIFPA L GTFSRN+R PKY IEF P+DSP++P D+ ES+ M
Sbjct: 7   LLCLVIVALSSSSHVWSDQIFPAHLVGTFSRNNREPKYKIEFLPEDSPFHPGDNLESMVM 66

Query: 64  PNKNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTPDELLEALKEQCFVRQ 123
            +K+G  +LCYLPK EK+ SG  + Q N+S++++E+++ VKLKTPDELL+ L E+C  RQ
Sbjct: 67  LDKHGNRFLCYLPKEEKATSGWTSSQQNISTVMMETQQLVKLKTPDELLQPLSEKCLFRQ 126

Query: 124 EGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNENLSDISTLKDPRSKDA 183
           EGWW+YEFC+QK ++Q H+EDE  +VQEF LG +D EATA  N+ +SD ST       DA
Sbjct: 127 EGWWSYEFCHQKYVRQLHVEDENKIVQEFFLGTFDPEATAAFNQTVSDAST-------DA 186

Query: 184 SQRSSKLEAYMK----------KQKLVRFVCSEPRAMINSITELSTCKYALTVRCPTLCK 243
           SQR      Y            ++  VRFVC+E RAM+ SITELSTCKYALTV+CPTLCK
Sbjct: 187 SQRYHS-HVYTNGTTCDLTGSPREVEVRFVCAETRAMVTSITELSTCKYALTVQCPTLCK 246

Query: 244 HPLFQEERPVWYIINCNVLPDDYKETKRVDELA 267
           HPLFQ E+PV + I+CN +P +   T+  +E A
Sbjct: 247 HPLFQLEKPVSHTIHCNAIPVEEDATRNKEEQA 271

BLAST of Lsi06G010700 vs. ExPASy Swiss-Prot
Match: Q67WM9 (Protein OS-9 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=OS9 PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.3e-58
Identity = 129/279 (46.24%), Postives = 181/279 (64.87%), Query Frame = 0

Query: 4   LMWLILLAGNLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHPQDSPYNPDDDQESVFM 63
           +++++  A      A ADQIF +  G  F RNSR P+Y++EFHP D+P+NP++ QESV M
Sbjct: 10  VLFVVAAAAAAALTAAADQIFTSS-GAPFGRNSREPRYHVEFHPVDAPFNPENGQESVPM 69

Query: 64  PNKNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTPDELLEALKEQCFVRQ 123
            +  GK+Y C+LP VE++K+ K  +  N +++I+ESE+RVK K PDELLE LK+QCF R 
Sbjct: 70  TSHVGKHYTCFLP-VEETKTMKSIIPQNATNVIIESERRVKPKDPDELLEILKDQCFYRH 129

Query: 124 EGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNENLSDISTLKDPRSKDA 183
           EGWW+YEFCY   ++Q H+E EK V+QE+VLG YDA+AT    EN +  S  +D    D 
Sbjct: 130 EGWWSYEFCYYGKIRQVHVEGEK-VIQEYVLGEYDADATDAYYENQTSDSADEDDNLIDT 189

Query: 184 SQRSSKLEAY----------MKKQKLVRFVCSEPRAMINSITELSTCKYALTVRCPTLCK 243
           S+R   +  Y          M ++  VRFVCSEP  +I+SI E+S+CKY LTV+ P LCK
Sbjct: 190 SKR-YHVHLYTNGTVCDLTDMPRETEVRFVCSEPTVVISSIKEISSCKYVLTVQSPMLCK 249

Query: 244 HPLFQEERPVWYIINCNVLPDDYKETKRVDELADEIVMV 273
           +PLFQ+E+     I+CN L  + + T   D L  E  ++
Sbjct: 250 NPLFQQEKRT-LSIHCNELLAEAEATVDDDSLPKEAQII 283

BLAST of Lsi06G010700 vs. ExPASy Swiss-Prot
Match: Q13438 (Protein OS-9 OS=Homo sapiens OX=9606 GN=OS9 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.4e-12
Identity = 68/271 (25.09%), Postives = 115/271 (42.44%), Query Frame = 0

Query: 10  LAGNLCHFALADQIFPAQL-GGTFSRNSR---GPKYNIEFHPQDSPYNPDDDQESVFMPN 69
           L  +L    L   + PA L GG  S N       +Y IE  P           + V + +
Sbjct: 6   LLSSLLGLLLLGLLLPASLTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSS 65

Query: 70  KNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTP-------DELLEALKE- 129
           K  + Y C LP                 +  +  ++  + +TP        ELL  +++ 
Sbjct: 66  KYKQRYECRLP-----------------AGAIHFQREREEETPAYQGPGIPELLSPMRDA 125

Query: 130 QCFVRQEGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDA-----EATANLNENLSDI 189
            C ++ + WWTYEFCY + ++Q+H+ED ++  +   LG Y +     + TA  ++     
Sbjct: 126 PCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQ---- 185

Query: 190 STLKDPRSKDASQRSSKLEAYMKKQKLVRFVCSEPRAM----INSITELSTCKYALTVRC 249
             LK   S+     S        ++  VRF+C E   +    I+ + E  +C Y LT+R 
Sbjct: 186 HRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRT 245

Query: 250 PTLCKHPLFQ---EERPVWYIINCNVLPDDY 257
           P LC HPL +      P   + + ++ P++Y
Sbjct: 246 PRLCPHPLLRPPPSAAPQAILCHPSLQPEEY 255

BLAST of Lsi06G010700 vs. ExPASy Swiss-Prot
Match: Q3MHX6 (Protein OS-9 OS=Bos taurus OX=9913 GN=OS9 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.6e-11
Identity = 66/250 (26.40%), Postives = 106/250 (42.40%), Query Frame = 0

Query: 10  LAGNLCHFALADQIFPAQL-GGTFSRNSR---GPKYNIEFHPQDSPYNPDDDQESVFMPN 69
           L  +L    L   + PA L GG  S N       +Y IE  P           + V + +
Sbjct: 6   LLSSLLGLLLLGLLLPATLTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQASDVVIVSS 65

Query: 70  KNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTP-------DELLEALKE- 129
           K  + Y C LP                 +  +  ++  + +TP        ELL  +K+ 
Sbjct: 66  KYKQRYECRLP-----------------AGAIHFQREREEETPAYQGPGIPELLSPMKDA 125

Query: 130 QCFVRQEGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDA-----EATANLNENLSDI 189
            C ++ + WWTYEFCY + ++Q+H+ED ++  +   LG Y +     + TA  ++     
Sbjct: 126 PCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQ---- 185

Query: 190 STLKDPRSKDASQRSSKLEAYMKKQKLVRFVCSEPRAM----INSITELSTCKYALTVRC 239
             LK   S+     S        ++  VRF+C E   +    I+ + E  +C Y LT+R 
Sbjct: 186 HRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRT 234

BLAST of Lsi06G010700 vs. ExPASy Swiss-Prot
Match: Q5RKH6 (Protein OS-9 OS=Rattus norvegicus OX=10116 GN=Os9 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 4.6e-11
Identity = 54/185 (29.19%), Postives = 92/185 (49.73%), Query Frame = 0

Query: 110 ELLEALKE-QCFVRQEGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNEN 169
           ELL  +++  C ++ + WWTYEFCY + ++Q+H+ED ++      LG Y  ++  N ++ 
Sbjct: 100 ELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGDVLYLGYY--QSAFNWDDE 159

Query: 170 LSDISTLKDPRSKDASQRS----SKLEAYMK-KQKLVRFVCSEPRAM----INSITELST 229
            +  S  K  R K    ++    SK +   K ++  VRF+C E   +    I+ + E  +
Sbjct: 160 TAKAS--KQHRLKRYHSQTYGNGSKCDLNGKPREAEVRFLCDEGAGISGDYIDRVDEPFS 219

Query: 230 CKYALTVRCPTLCKHPLFQ---EERPVWYIINCNVLPDDY-------KETKRVDELADEI 275
           C Y L++R   LC HPL +      P   + +  + PD+Y        E+K+ +E   E 
Sbjct: 220 CSYVLSIRTSRLCPHPLLRPPASAAPQAILCHPALQPDEYMAYLQRQAESKQHEEKVTEE 279

BLAST of Lsi06G010700 vs. ExPASy TrEMBL
Match: A0A1S3B9X7 (protein OS-9 homolog isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487399 PE=4 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 9.6e-137
Identity = 250/294 (85.03%), Postives = 264/294 (89.80%), Query Frame = 0

Query: 1   MTKLMWLILLAGNLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHPQDSPYNPDDDQES 60
           MT+L+WLILLAG LCHFALADQIFPAQLGGTFSRNSRGPKYNIEFH QDSPYNPD+DQES
Sbjct: 1   MTQLLWLILLAGYLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHQQDSPYNPDEDQES 60

Query: 61  VFMPNKNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTPDELLEALKEQCF 120
           VFMPNKNGKNYLCYLPKVEKSKSGKP++Q+NMSSMIVESEKRVKLKTPDELLEALKEQCF
Sbjct: 61  VFMPNKNGKNYLCYLPKVEKSKSGKPSIQLNMSSMIVESEKRVKLKTPDELLEALKEQCF 120

Query: 121 VRQEGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNENLSDISTLKDPRS 180
           VRQEGWWTYEFCYQKTL+QFHLEDEK VVQEF+LGVYDAEATA LNENLSDISTLKDPRS
Sbjct: 121 VRQEGWWTYEFCYQKTLRQFHLEDEK-VVQEFILGVYDAEATAKLNENLSDISTLKDPRS 180

Query: 181 KDASQRSSKLE---------AYMKKQKLVRFVCSE-PRAMINSITELSTCKYALTVRCPT 240
           KDASQR                  ++  VRFVCSE PRAMINSITELSTCKYALTVRCPT
Sbjct: 181 KDASQRYHAHHYTNGTMCDLTNQPRETEVRFVCSEPPRAMINSITELSTCKYALTVRCPT 240

Query: 241 LCKHPLFQEERPVWYIINCNVLPDDYKETKRVDELADEIVMVTDVKYPKNESED 285
           LCKH LF+EERPVWYIINCN LPDDYKET+R +E  DEIVMVTD+KYPKNESED
Sbjct: 241 LCKHMLFEEERPVWYIINCNELPDDYKETERSEESTDEIVMVTDIKYPKNESED 293

BLAST of Lsi06G010700 vs. ExPASy TrEMBL
Match: A0A6J1EY24 (protein OS-9 homolog OS=Cucurbita moschata OX=3662 GN=LOC111439427 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 8.1e-136
Identity = 243/293 (82.94%), Postives = 263/293 (89.76%), Query Frame = 0

Query: 1   MTKLMWLILLAGNLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHPQDSPYNPDDDQES 60
           M KL+WL+LLAG  CH  LADQIFPAQLGGTFSRNSRGPKYNIEFHP+DSPYNPDDDQES
Sbjct: 1   MRKLLWLVLLAGIFCHCVLADQIFPAQLGGTFSRNSRGPKYNIEFHPEDSPYNPDDDQES 60

Query: 61  VFMPNKNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTPDELLEALKEQCF 120
           VFMPNKNGKNY+C+LPK+EKSKSGKP +QVN+SSMIVESEKRVKLKTPDELLEALKEQCF
Sbjct: 61  VFMPNKNGKNYVCFLPKMEKSKSGKPAIQVNVSSMIVESEKRVKLKTPDELLEALKEQCF 120

Query: 121 VRQEGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNENLSDISTLKDPRS 180
           VRQEGWWTYEFCYQKTL+QFHLED+K VVQEFVLGVYDAEATANL+ENLSD+STLKDPRS
Sbjct: 121 VRQEGWWTYEFCYQKTLRQFHLEDDK-VVQEFVLGVYDAEATANLHENLSDVSTLKDPRS 180

Query: 181 KDASQRSSKLE---------AYMKKQKLVRFVCSEPRAMINSITELSTCKYALTVRCPTL 240
           KDASQR                  ++  VRFVCSEPRAMINSITELSTCKYALTVRCPTL
Sbjct: 181 KDASQRYHAHHYTNGTMCDLTNQPRETEVRFVCSEPRAMINSITELSTCKYALTVRCPTL 240

Query: 241 CKHPLFQEERPVWYIINCNVLPDDYKETKRVDELADEIVMVTDVKYPKNESED 285
           CKHPLFQEERPVWY INCNVLP+DYKET++ +EL DEIVMVTDVKYPKNES++
Sbjct: 241 CKHPLFQEERPVWYTINCNVLPEDYKETEQTEELDDEIVMVTDVKYPKNESDE 292

BLAST of Lsi06G010700 vs. ExPASy TrEMBL
Match: A0A1S3B986 (protein OS-9 homolog isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487399 PE=4 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.4e-135
Identity = 250/295 (84.75%), Postives = 264/295 (89.49%), Query Frame = 0

Query: 1   MTKLMWLILLAGNLCHFALADQIFPAQL-GGTFSRNSRGPKYNIEFHPQDSPYNPDDDQE 60
           MT+L+WLILLAG LCHFALADQIFPAQL GGTFSRNSRGPKYNIEFH QDSPYNPD+DQE
Sbjct: 1   MTQLLWLILLAGYLCHFALADQIFPAQLAGGTFSRNSRGPKYNIEFHQQDSPYNPDEDQE 60

Query: 61  SVFMPNKNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTPDELLEALKEQC 120
           SVFMPNKNGKNYLCYLPKVEKSKSGKP++Q+NMSSMIVESEKRVKLKTPDELLEALKEQC
Sbjct: 61  SVFMPNKNGKNYLCYLPKVEKSKSGKPSIQLNMSSMIVESEKRVKLKTPDELLEALKEQC 120

Query: 121 FVRQEGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNENLSDISTLKDPR 180
           FVRQEGWWTYEFCYQKTL+QFHLEDEK VVQEF+LGVYDAEATA LNENLSDISTLKDPR
Sbjct: 121 FVRQEGWWTYEFCYQKTLRQFHLEDEK-VVQEFILGVYDAEATAKLNENLSDISTLKDPR 180

Query: 181 SKDASQRSSKLE---------AYMKKQKLVRFVCSE-PRAMINSITELSTCKYALTVRCP 240
           SKDASQR                  ++  VRFVCSE PRAMINSITELSTCKYALTVRCP
Sbjct: 181 SKDASQRYHAHHYTNGTMCDLTNQPRETEVRFVCSEPPRAMINSITELSTCKYALTVRCP 240

Query: 241 TLCKHPLFQEERPVWYIINCNVLPDDYKETKRVDELADEIVMVTDVKYPKNESED 285
           TLCKH LF+EERPVWYIINCN LPDDYKET+R +E  DEIVMVTD+KYPKNESED
Sbjct: 241 TLCKHMLFEEERPVWYIINCNELPDDYKETERSEESTDEIVMVTDIKYPKNESED 294

BLAST of Lsi06G010700 vs. ExPASy TrEMBL
Match: A0A0A0LWI4 (PRKCSH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G264000 PE=4 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 4.5e-134
Identity = 249/295 (84.41%), Postives = 262/295 (88.81%), Query Frame = 0

Query: 1   MTKLMWLILLAGNLCHFALADQIFPAQL-GGTFSRNSRGPKYNIEFHPQDSPYNPDDDQE 60
           MT+L+WLILLAG LCHFALADQIFPAQL GGTFSRNSRGPKYNIEFH Q+SPYNPD+DQE
Sbjct: 1   MTQLLWLILLAGYLCHFALADQIFPAQLAGGTFSRNSRGPKYNIEFHQQNSPYNPDEDQE 60

Query: 61  SVFMPNKNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTPDELLEALKEQC 120
           SVFMPNKNGKNYLCYLPKVEKSKSGKP++QVNMSSMIVESEKRVKLKTPDELLEALKEQC
Sbjct: 61  SVFMPNKNGKNYLCYLPKVEKSKSGKPSIQVNMSSMIVESEKRVKLKTPDELLEALKEQC 120

Query: 121 FVRQEGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNENLSDISTLKDPR 180
           FVRQEGWWTYEFCYQK L+QFHLEDEK VVQEFVLGVYD EATA LNENLSDISTLKDPR
Sbjct: 121 FVRQEGWWTYEFCYQKALRQFHLEDEK-VVQEFVLGVYDPEATAKLNENLSDISTLKDPR 180

Query: 181 SKDASQRSSKLE---------AYMKKQKLVRFVCSE-PRAMINSITELSTCKYALTVRCP 240
           SKDASQR                  ++  VRFVCSE PRAMINSITELSTCKYALTVRCP
Sbjct: 181 SKDASQRYHAHHYTNGTMCDLTNQPRETEVRFVCSEPPRAMINSITELSTCKYALTVRCP 240

Query: 241 TLCKHPLFQEERPVWYIINCNVLPDDYKETKRVDELADEIVMVTDVKYPKNESED 285
           TLCKH LF+EERPVWYIINCN LPDDYKET+R +E  DEIVMVTD+KYPKNESED
Sbjct: 241 TLCKHMLFEEERPVWYIINCNELPDDYKETERSEESTDEIVMVTDIKYPKNESED 294

BLAST of Lsi06G010700 vs. ExPASy TrEMBL
Match: A0A6J1HVT6 (protein OS-9 homolog OS=Cucurbita maxima OX=3661 GN=LOC111467906 PE=4 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.4e-132
Identity = 237/293 (80.89%), Postives = 260/293 (88.74%), Query Frame = 0

Query: 1   MTKLMWLILLAGNLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHPQDSPYNPDDDQES 60
           M KL+ L+LL GN CH  LADQIFPAQLGGTFSRNSRG KYNIEFHP+DSPYNPDDDQES
Sbjct: 1   MRKLLCLVLLVGNFCHSVLADQIFPAQLGGTFSRNSRGLKYNIEFHPEDSPYNPDDDQES 60

Query: 61  VFMPNKNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTPDELLEALKEQCF 120
           VFMPNKNGKNY+C+LPK+EKSK+GKP +QVNMSSMIVESEKRVKLKTPDELLEALKEQCF
Sbjct: 61  VFMPNKNGKNYMCFLPKMEKSKNGKPAIQVNMSSMIVESEKRVKLKTPDELLEALKEQCF 120

Query: 121 VRQEGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNENLSDISTLKDPRS 180
           VRQEGWWTYEFCYQKTL+QFHLED+K VVQEFVLGVYDA+ATANL+ENLSD+STLKDPRS
Sbjct: 121 VRQEGWWTYEFCYQKTLRQFHLEDDK-VVQEFVLGVYDADATANLHENLSDVSTLKDPRS 180

Query: 181 KDASQRSSKLE---------AYMKKQKLVRFVCSEPRAMINSITELSTCKYALTVRCPTL 240
           KDASQR                  ++  VRFVCSEPRAMINSITELSTCKY+LTVRCPTL
Sbjct: 181 KDASQRYHAHHYTNGTMCDLTNQPRETEVRFVCSEPRAMINSITELSTCKYSLTVRCPTL 240

Query: 241 CKHPLFQEERPVWYIINCNVLPDDYKETKRVDELADEIVMVTDVKYPKNESED 285
           CKHPLFQEERPVWY INCNVLP+ YKET++ +EL DEIVMVTD+KYPKNES++
Sbjct: 241 CKHPLFQEERPVWYTINCNVLPEGYKETEQTEELDDEIVMVTDIKYPKNESDE 292

BLAST of Lsi06G010700 vs. NCBI nr
Match: XP_038878474.1 (protein OS-9 homolog isoform X2 [Benincasa hispida])

HSP 1 Score: 520.0 bits (1338), Expect = 1.3e-143
Identity = 263/293 (89.76%), Postives = 269/293 (91.81%), Query Frame = 0

Query: 1   MTKLMWLILLAGNLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHPQDSPYNPDDDQES 60
           MT LM LILLAGNLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHPQDSPYNPDDDQES
Sbjct: 1   MTNLMLLILLAGNLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHPQDSPYNPDDDQES 60

Query: 61  VFMPNKNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTPDELLEALKEQCF 120
           VFMPNKNGKNYLCYLPKVEKSKSGKPTVQ+NMSSMIVESEKRVKLKTPDELLEALKEQCF
Sbjct: 61  VFMPNKNGKNYLCYLPKVEKSKSGKPTVQLNMSSMIVESEKRVKLKTPDELLEALKEQCF 120

Query: 121 VRQEGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNENLSDISTLKDPRS 180
           VRQEGWWTYEFCYQKTL+QFHLEDEK VVQEFVLGVYDAEATANLNENLSDISTLKDPRS
Sbjct: 121 VRQEGWWTYEFCYQKTLRQFHLEDEK-VVQEFVLGVYDAEATANLNENLSDISTLKDPRS 180

Query: 181 KDASQRSSKLE---------AYMKKQKLVRFVCSEPRAMINSITELSTCKYALTVRCPTL 240
           KDASQR                  ++  VRFVCSEPRAMINSITELSTCKYALTVRCPTL
Sbjct: 181 KDASQRYHAHHYTNGTMCDLTNQPRETEVRFVCSEPRAMINSITELSTCKYALTVRCPTL 240

Query: 241 CKHPLFQEERPVWYIINCNVLPDDYKETKRVDELADEIVMVTDVKYPKNESED 285
           CKHPLFQEERPVWYIINCNVLPDDYKET+R +ELADEIVMVTDVKYPKNESED
Sbjct: 241 CKHPLFQEERPVWYIINCNVLPDDYKETERSEELADEIVMVTDVKYPKNESED 292

BLAST of Lsi06G010700 vs. NCBI nr
Match: XP_038878473.1 (protein OS-9 homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 515.4 bits (1326), Expect = 3.2e-142
Identity = 263/294 (89.46%), Postives = 269/294 (91.50%), Query Frame = 0

Query: 1   MTKLMWLILLAGNLCHFALADQIFPAQL-GGTFSRNSRGPKYNIEFHPQDSPYNPDDDQE 60
           MT LM LILLAGNLCHFALADQIFPAQL GGTFSRNSRGPKYNIEFHPQDSPYNPDDDQE
Sbjct: 1   MTNLMLLILLAGNLCHFALADQIFPAQLAGGTFSRNSRGPKYNIEFHPQDSPYNPDDDQE 60

Query: 61  SVFMPNKNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTPDELLEALKEQC 120
           SVFMPNKNGKNYLCYLPKVEKSKSGKPTVQ+NMSSMIVESEKRVKLKTPDELLEALKEQC
Sbjct: 61  SVFMPNKNGKNYLCYLPKVEKSKSGKPTVQLNMSSMIVESEKRVKLKTPDELLEALKEQC 120

Query: 121 FVRQEGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNENLSDISTLKDPR 180
           FVRQEGWWTYEFCYQKTL+QFHLEDEK VVQEFVLGVYDAEATANLNENLSDISTLKDPR
Sbjct: 121 FVRQEGWWTYEFCYQKTLRQFHLEDEK-VVQEFVLGVYDAEATANLNENLSDISTLKDPR 180

Query: 181 SKDASQRSSKLE---------AYMKKQKLVRFVCSEPRAMINSITELSTCKYALTVRCPT 240
           SKDASQR                  ++  VRFVCSEPRAMINSITELSTCKYALTVRCPT
Sbjct: 181 SKDASQRYHAHHYTNGTMCDLTNQPRETEVRFVCSEPRAMINSITELSTCKYALTVRCPT 240

Query: 241 LCKHPLFQEERPVWYIINCNVLPDDYKETKRVDELADEIVMVTDVKYPKNESED 285
           LCKHPLFQEERPVWYIINCNVLPDDYKET+R +ELADEIVMVTDVKYPKNESED
Sbjct: 241 LCKHPLFQEERPVWYIINCNVLPDDYKETERSEELADEIVMVTDVKYPKNESED 293

BLAST of Lsi06G010700 vs. NCBI nr
Match: XP_023530346.1 (protein OS-9 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 496.5 bits (1277), Expect = 1.5e-136
Identity = 244/293 (83.28%), Postives = 264/293 (90.10%), Query Frame = 0

Query: 1   MTKLMWLILLAGNLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHPQDSPYNPDDDQES 60
           M KL+WL+LLAG  CHF LADQIFPAQLGGTFSRNSRGPKYNIEFHP+DSPYNPDDDQES
Sbjct: 1   MRKLLWLVLLAGIFCHFVLADQIFPAQLGGTFSRNSRGPKYNIEFHPEDSPYNPDDDQES 60

Query: 61  VFMPNKNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTPDELLEALKEQCF 120
           VFMPNKNGKNY+C+LPK+EKSKSGKP +QVN+SSMIVESEKRVKLKTPDELLEALKEQCF
Sbjct: 61  VFMPNKNGKNYMCFLPKMEKSKSGKPAIQVNVSSMIVESEKRVKLKTPDELLEALKEQCF 120

Query: 121 VRQEGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNENLSDISTLKDPRS 180
           VRQEGWWTYEFCYQKTL+QFHLED+K VVQEFVLGVYDAEATANL+ENLSD+STLKDPRS
Sbjct: 121 VRQEGWWTYEFCYQKTLRQFHLEDDK-VVQEFVLGVYDAEATANLHENLSDVSTLKDPRS 180

Query: 181 KDASQRSSKLE---------AYMKKQKLVRFVCSEPRAMINSITELSTCKYALTVRCPTL 240
           KDASQR                  ++  VRFVCSEPRAMINSITELSTCKYALTVRCPTL
Sbjct: 181 KDASQRYHAHHYTNGTMCDLTNQPRETEVRFVCSEPRAMINSITELSTCKYALTVRCPTL 240

Query: 241 CKHPLFQEERPVWYIINCNVLPDDYKETKRVDELADEIVMVTDVKYPKNESED 285
           CKHPLFQEERPVWY INCNVLP+DYKET++ +EL DEIVMVTDVKYPKNES++
Sbjct: 241 CKHPLFQEERPVWYTINCNVLPEDYKETEQTEELDDEIVMVTDVKYPKNESDE 292

BLAST of Lsi06G010700 vs. NCBI nr
Match: XP_008443924.1 (PREDICTED: protein OS-9 homolog isoform X2 [Cucumis melo])

HSP 1 Score: 496.1 bits (1276), Expect = 2.0e-136
Identity = 250/294 (85.03%), Postives = 264/294 (89.80%), Query Frame = 0

Query: 1   MTKLMWLILLAGNLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHPQDSPYNPDDDQES 60
           MT+L+WLILLAG LCHFALADQIFPAQLGGTFSRNSRGPKYNIEFH QDSPYNPD+DQES
Sbjct: 1   MTQLLWLILLAGYLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHQQDSPYNPDEDQES 60

Query: 61  VFMPNKNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTPDELLEALKEQCF 120
           VFMPNKNGKNYLCYLPKVEKSKSGKP++Q+NMSSMIVESEKRVKLKTPDELLEALKEQCF
Sbjct: 61  VFMPNKNGKNYLCYLPKVEKSKSGKPSIQLNMSSMIVESEKRVKLKTPDELLEALKEQCF 120

Query: 121 VRQEGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNENLSDISTLKDPRS 180
           VRQEGWWTYEFCYQKTL+QFHLEDEK VVQEF+LGVYDAEATA LNENLSDISTLKDPRS
Sbjct: 121 VRQEGWWTYEFCYQKTLRQFHLEDEK-VVQEFILGVYDAEATAKLNENLSDISTLKDPRS 180

Query: 181 KDASQRSSKLE---------AYMKKQKLVRFVCSE-PRAMINSITELSTCKYALTVRCPT 240
           KDASQR                  ++  VRFVCSE PRAMINSITELSTCKYALTVRCPT
Sbjct: 181 KDASQRYHAHHYTNGTMCDLTNQPRETEVRFVCSEPPRAMINSITELSTCKYALTVRCPT 240

Query: 241 LCKHPLFQEERPVWYIINCNVLPDDYKETKRVDELADEIVMVTDVKYPKNESED 285
           LCKH LF+EERPVWYIINCN LPDDYKET+R +E  DEIVMVTD+KYPKNESED
Sbjct: 241 LCKHMLFEEERPVWYIINCNELPDDYKETERSEESTDEIVMVTDIKYPKNESED 293

BLAST of Lsi06G010700 vs. NCBI nr
Match: XP_022932897.1 (protein OS-9 homolog [Cucurbita moschata])

HSP 1 Score: 493.0 bits (1268), Expect = 1.7e-135
Identity = 243/293 (82.94%), Postives = 263/293 (89.76%), Query Frame = 0

Query: 1   MTKLMWLILLAGNLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHPQDSPYNPDDDQES 60
           M KL+WL+LLAG  CH  LADQIFPAQLGGTFSRNSRGPKYNIEFHP+DSPYNPDDDQES
Sbjct: 1   MRKLLWLVLLAGIFCHCVLADQIFPAQLGGTFSRNSRGPKYNIEFHPEDSPYNPDDDQES 60

Query: 61  VFMPNKNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTPDELLEALKEQCF 120
           VFMPNKNGKNY+C+LPK+EKSKSGKP +QVN+SSMIVESEKRVKLKTPDELLEALKEQCF
Sbjct: 61  VFMPNKNGKNYVCFLPKMEKSKSGKPAIQVNVSSMIVESEKRVKLKTPDELLEALKEQCF 120

Query: 121 VRQEGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNENLSDISTLKDPRS 180
           VRQEGWWTYEFCYQKTL+QFHLED+K VVQEFVLGVYDAEATANL+ENLSD+STLKDPRS
Sbjct: 121 VRQEGWWTYEFCYQKTLRQFHLEDDK-VVQEFVLGVYDAEATANLHENLSDVSTLKDPRS 180

Query: 181 KDASQRSSKLE---------AYMKKQKLVRFVCSEPRAMINSITELSTCKYALTVRCPTL 240
           KDASQR                  ++  VRFVCSEPRAMINSITELSTCKYALTVRCPTL
Sbjct: 181 KDASQRYHAHHYTNGTMCDLTNQPRETEVRFVCSEPRAMINSITELSTCKYALTVRCPTL 240

Query: 241 CKHPLFQEERPVWYIINCNVLPDDYKETKRVDELADEIVMVTDVKYPKNESED 285
           CKHPLFQEERPVWY INCNVLP+DYKET++ +EL DEIVMVTDVKYPKNES++
Sbjct: 241 CKHPLFQEERPVWYTINCNVLPEDYKETEQTEELDDEIVMVTDVKYPKNESDE 292

BLAST of Lsi06G010700 vs. TAIR 10
Match: AT5G35080.1 (INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Mannose-6-phosphate receptor, binding (InterPro:IPR009011), Glucosidase II beta subunit-like (InterPro:IPR012913); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 278.1 bits (710), Expect = 7.9e-75
Identity = 144/273 (52.75%), Postives = 192/273 (70.33%), Query Frame = 0

Query: 4   LMWLILLAGNLCHFALADQIFPAQLGGTFSRNSRGPKYNIEFHPQDSPYNPDDDQESVFM 63
           L+ L+++A +      +DQIFPA L GTFSRN+R PKY IEF P+DSP++P D+ ES+ M
Sbjct: 7   LLCLVIVALSSSSHVWSDQIFPAHLVGTFSRNNREPKYKIEFLPEDSPFHPGDNLESMVM 66

Query: 64  PNKNGKNYLCYLPKVEKSKSGKPTVQVNMSSMIVESEKRVKLKTPDELLEALKEQCFVRQ 123
            +K+G  +LCYLPK EK+ SG  + Q N+S++++E+++ VKLKTPDELL+ L E+C  RQ
Sbjct: 67  LDKHGNRFLCYLPKEEKATSGWTSSQQNISTVMMETQQLVKLKTPDELLQPLSEKCLFRQ 126

Query: 124 EGWWTYEFCYQKTLKQFHLEDEKVVVQEFVLGVYDAEATANLNENLSDISTLKDPRSKDA 183
           EGWW+YEFC+QK ++Q H+EDE  +VQEF LG +D EATA  N+ +SD ST       DA
Sbjct: 127 EGWWSYEFCHQKYVRQLHVEDENKIVQEFFLGTFDPEATAAFNQTVSDAST-------DA 186

Query: 184 SQRSSKLEAYMK----------KQKLVRFVCSEPRAMINSITELSTCKYALTVRCPTLCK 243
           SQR      Y            ++  VRFVC+E RAM+ SITELSTCKYALTV+CPTLCK
Sbjct: 187 SQRYHS-HVYTNGTTCDLTGSPREVEVRFVCAETRAMVTSITELSTCKYALTVQCPTLCK 246

Query: 244 HPLFQEERPVWYIINCNVLPDDYKETKRVDELA 267
           HPLFQ E+PV + I+CN +P +   T+  +E A
Sbjct: 247 HPLFQLEKPVSHTIHCNAIPVEEDATRNKEEQA 271

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GWH31.1e-7352.75Protein OS-9 homolog OS=Arabidopsis thaliana OX=3702 GN=OS9 PE=1 SV=1[more]
Q67WM92.3e-5846.24Protein OS-9 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=OS9 PE=2 SV=1[more]
Q134385.4e-1225.09Protein OS-9 OS=Homo sapiens OX=9606 GN=OS9 PE=1 SV=1[more]
Q3MHX61.6e-1126.40Protein OS-9 OS=Bos taurus OX=9913 GN=OS9 PE=2 SV=1[more]
Q5RKH64.6e-1129.19Protein OS-9 OS=Rattus norvegicus OX=10116 GN=Os9 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3B9X79.6e-13785.03protein OS-9 homolog isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487399 PE=4 SV=... [more]
A0A6J1EY248.1e-13682.94protein OS-9 homolog OS=Cucurbita moschata OX=3662 GN=LOC111439427 PE=4 SV=1[more]
A0A1S3B9862.4e-13584.75protein OS-9 homolog isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487399 PE=4 SV=... [more]
A0A0A0LWI44.5e-13484.41PRKCSH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G264000 PE=4... [more]
A0A6J1HVT62.4e-13280.89protein OS-9 homolog OS=Cucurbita maxima OX=3661 GN=LOC111467906 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038878474.11.3e-14389.76protein OS-9 homolog isoform X2 [Benincasa hispida][more]
XP_038878473.13.2e-14289.46protein OS-9 homolog isoform X1 [Benincasa hispida][more]
XP_023530346.11.5e-13683.28protein OS-9 homolog [Cucurbita pepo subsp. pepo][more]
XP_008443924.12.0e-13685.03PREDICTED: protein OS-9 homolog isoform X2 [Cucumis melo][more]
XP_022932897.11.7e-13582.94protein OS-9 homolog [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G35080.17.9e-7552.75INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRES... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012913Protein OS9-like domainPFAMPF07915PRKCSHcoord: 118..173
e-value: 5.2E-13
score: 49.8
IPR009011Mannose-6-phosphate receptor binding domain superfamilyGENE3D2.70.130.10coord: 115..228
e-value: 5.8E-19
score: 70.7
IPR045149Protein OS-9-likePANTHERPTHR15414OS-9-RELATEDcoord: 28..260
NoneNo IPR availablePANTHERPTHR15414:SF0PROTEIN OS-9coord: 28..260
IPR044865MRH domainPROSITEPS51914MRHcoord: 117..234
score: 11.123347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G010700.1Lsi06G010700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030968 endoplasmic reticulum unfolded protein response
biological_process GO:0030970 retrograde protein transport, ER to cytosol
biological_process GO:0030433 ubiquitin-dependent ERAD pathway
cellular_component GO:0005788 endoplasmic reticulum lumen