Lsi06G009420 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G009420
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionacyl-protein thioesterase 2
Locationchr06: 19350104 .. 19356094 (+)
RNA-Seq ExpressionLsi06G009420
SyntenyLsi06G009420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTCATCGAATATTAAAAAAACTCAACCTTTTCCTATGTTTGGGAGTACCCCACCAGCGCTGCAGCTATGGCAAATTAAACACAACAGGTGCGGACCCAGCGGGGCGGGGACACAACACACACACACCCCTCAAAAGAAAACTGTGTGTGAGAGAGAAAGAGAGAGAGAGAGAAACAATCAATACAGCGACGAGAACAACAAGACATAGCCATAAACACACCCACTTTAAACCCCTCTCTCTGGCTTTGGCTGTTCCCCCACCCAGGTTCACTTTCTTTCTCTCAAATCTTCAACACCCTTCTGATTTTCGCCTCTAAATTCATTGTTTCTTCATGGGTCATTGATGTTTTCCTGCTTTAATTTGTTTTTCTTTTGAGTTTTTGAAATGGAATACATGATGGGGGTGTTTTTCCCCCCTTTTTTTGAATGTTTTGTCATTTGGGTTGTTGGATTTCATTTGGGGGATGGAATGGTTTGGTGATGGTGAGAAGTTTTTGAATCTTGGGTAGTGTTCAGTCTGTGGGGTTTTTGAAGAGTTGTGATTTGTTTGTGGGATTTTTTTTGGTTGCTATTGGGCTGTAGTCATAGGGGAAGCTTGTTGTAATCTCGTTGGATTTGGTTTGAAATGTATGTATTTTTGTGGTATCTCATTCTTCATTCTTTCTTCCATGGAGGTTTGAAAGGGGACTGAATCCTGGAAATTTAGTCAAAAGGATTGAAATGAATTATAGTTTCTCGAATGAAGCCGGCGTCCTTGCATTGTTTTTAACGAGCTTTGTGATTGGATGTTGTTTTGACGAGATTTCGTCCTTGGATGATATTTAATTCAGCAAGTTTCTCGAAATAATTTTAACAGTGTGTAGGTGGCTTATTATGATTTGTTTTTTTTACCCCCAGCAGGATCAATTCATAGTCAATCTCTTAAGGTGGTGGTTCACTTGGCTTTTATATTGCGAATATGAGTTTTGCTGGCTCTTCCGTTGCTGCTGGTTAGTGTACTTGTCCCTCTGAATTCTCCTGTCCTGTTTGCTGATTAGTTTTTCTTTCTTTGATTTTAAGGTGTTTTATTCTACTGCATATCTCATTTTGGATTCATCTCCTTTTCCATCCTTAGTCCTTAAACATTACTGTTTTATTCAAGAAACGATACTCTAATTTCTGTTTGTGGGAGTAGCTCTATATCGTTCCAATTTTATCGGATGTCCTCGAAGGAAGAGTTTTGTGGAGACTTTCACTCTTTGTTGGAGGAGGTCTCGATGACTTTTTCTTTTTGCGGGATAAGGGGAGAGTTTTGTGGCAGGCTTGTTGTTTTGCTATTTCGTGGGGAGTGTGGGTTCATTCTTTTGGATTTGAGATCTTTCCTATAGTTGAAGGACTCTTGTGGTTTTTTTTTTTTTATTTAAATTACTTTTTTATGGTCTTCTTTATTCTCTCATTTTTCTTGATGAAAGCTTGGTGTCTTAAAAAAATTGTGGGGTAGCTAGGCATTATGTTGGCTTCACTTTAAGATATACTATTTTGTGGAAGTTGAGCTATCTTGCTTGAGCATGTCATGGTCACTTTCAGGTGGTAGAGCTGCCAAAAAGGCAATTGAGTTTGGTAAAACATACGTAGTGAGGCCCAAGGGAAAACACCAAGCTACCGTTGTCTGGCTTCATGGTCTTGGTGATAATGGCTCAAGGTTCTCTTCCTTCTTTCTTTCTGTTCTTTTCTTCTTCTCTTAAATTTTTTGCATACAGCATGAATTCATTTCCCTAGTTTATCTGCATGCGGATCATTCAATTAGACATTTTCCTAGTGTTGCAATTACATAATTTTCAAGCATTCATGTTTCATCACTTCTTGTCTTGGTCTTCACTGACAAATTTCCTTCAGAGACTATCTTATTCGACCTTCTCTCCCCATTGTTTTTGAAGCTAGATCGAATGTTTGAACTTTCCCCATTCTGCATTGACTGTACAAGAAATGGAAAGACATTGATTATTCATGTGTCCACTGGCCTTCTGCATTTGCTAGCTCCACTTACTTTCTCAGTTTGTCTACTCTCTCTCGAGCTTCCCCTCAGGATTTTCCGATTGATGTGATGAACTGATTATACAATTTGTTTTGTGGTTTTCTGATTGGTGGTTAAAGTCTAAAATAAAAACCAAAAAAAAAAAAAATTTATTAGGAAACAATTTCATCGATAGAATGGAACTTTTAAAAAAGAAATGAATTTCCATTCAATGGAGTTACAAAAAAAGACCTCTAATTGGATATGAGTGTTATGACTATTGAAAAAGGAGAAACATGTTTTCACTAATAAACAACTAAAAAGATAAGATCAATCAAATCTGCAATCAGCCTTTCTTTTTCTTGGAATATTCTATCATTTTGCTCCCAAAAGTAGGCTCGGAAAATGTTAAACCAAATAATCCTTTTCTCAGCTGTGAAAGGGTGATCTATAAGGATGAGAGACAGCCATCTAATAAGGTCTACTGGGGTAGTAACCGACCAGTTAAAAGCGCTGAAAAAGCTTGACCAAATCATTCTGGACACATTGCAGTGGATGAAAAGATGGATAAAGAACAAACTGCAGAAAATGTCTCCGTAATCGATCTTGGTAATCAAACAAGCATGGCTGTGCTCCCACATTGGAAATTTCACCTTTTTGAGTAGAAATCCTTCCAATTGGATATAAGAGCGTTAAGACTATAGTTGCAGCGCAAAAAAGACAAAGGCTTTTGGTATAAATGAAGCAAAGTTCTAAGTTTGTGTATTTTGAGTAAAAGAAGGCTTTTATATATGCAGGAGTGTGGAAAGTTTCTTGACATTATTCTTTCTTTGGGTCTAGTTAGAGCTCTTCAGGTGAAACGTTTCTAATTACTCTCCTTTCAACATTAATACTATTAGGAGGCCTTTTTTATAACCTCTAGGCTATGGTTTAGATTGTTAAACACCAATCAATCCAAAAGCTTAAGCGGATAGTTATTATAAATTTAATATTATAACAACACTACCCTTCTCCTCACTTGTGGGCTTGAAAATTTGTTGAAGACCCAACAAGTGGAGTTCAATATTAATTAGAAAGAAAATGACTTTATAGGGGTTTGAACTGGACCTCCTTTGATACCATGTCAAACACTAATCAACCCAAAAGCTTAAGCCAATGATTATGGTAAATTTAATGTTATATCAACACTCTAACATGGATCATACTGTCTCCCCCCCTCCCTCTTCTTCAACCTTTTTGTAATTTTGTGTTTCCCATGCAAAGATTATTTCCTCTGGTACAAAATCATAGACCCGTATTAAGGAACTTGAATTTCCATTGTCTTCTCTGATTTAATTAGTGTGATATGACAAATGTTCAGTTGTTAGGAGAACAGACCTTTTAAAGTTCCATATCCTATTTCTTTCCAAGTCACCTAGATGTTGTACAATACGCTCTCTAGGTTGGAGTCACAGGGAAGAGGGAGGGATCATCTTTCATTATTCCTCAGCATTTATTTAAACACACACTCCAAGATTTTATCTATAAGGACTTCTTTTGGCCATAAACCTATGCAGATTTTTTGACCCTCGTAGAAATTGATGAATGGTGTGATTGAGTTGTTTTCATGGTTGGAAGTGTTATTATTGTCGAAGTGGCTTCATAATCTGACCTTTATCCGTATATCAAAAAGTTAACCTTGTGAAACTTTTAATATAAGGCTGTATTTATTTGAGCTTTTCACCTCTTTTTTTGGTATGATTCATGAGAGTGCTAAGGATACTTCTCCTGGTAGGTTTTTAATTCAAATTTTTAACTGACAGACTTCCACTTCAACATCATCCTTAGGATAAGACCTTAGAAGAATATAATGTGAAACATGACATGTACTATTTTGTTATAATACCTCGCAATTTTATTCAGTAGAATGTCATGTTTAGAAATGTTTCAATTTTGTTTGACAGCTGGTCGCAACTATTGGAGACGCTTCCCTTACCAAATGTAAGAATATGAAGCTTATCAAAGTATTTAGAAGCATCTATTCCAAATTTATTCTATAATTTCTACTCAGTCCCCTTTCTTTTTGCAACTCTTTTTTAATAATCTAAGAGGTAAAAACTAGATGGAAAAATCCATTGCTGGAAGATGAAATCGTTTATTTAGTGTTATTTTATAAACTTGGAGTTACTGATGCAGATTAAATGGATATGTCCGACTGCGCCTACTCGACCAATAGCTTTATTTGGTGGCTTTCCATCTACTGCATGTAAGCTTACTGAAATCCCTTTTTCTTTACGTAAGATCACTCAAACTGGGAGCTCTTGTTATTTATGTATTCTACAATAGCAAGGGCATCAAGAGTGTTATCCTACCTGCTCTTGCATGAAAATCTCTTTGCCCCTCCTACATTTGAAGCAAAAGCTTTATTGAAAGAGGCGAAACTCACTTGGACAAACATGTATTACAGTGTGAAACAAAAGTTACAATGAGATGTTTTTGTTAAACCAGGGTTTGATGTGGAAGATCTTTCAGAAGAAGGTCCGGATGATTTAGAGGGTTTGGATGCTTCAGCTGCACATGTTGCATATTTGTTATCAACAGAGCCAGCTGACAGTATGTTTCTCCTGCAATTTTATATCTGTGGCTATCATTGATCCAACGTTTTCTTTAAATTCTAAGATGGAGTTTCAATAGCATTTTAGTGATTAGACCACTTCAACTCCTATTTCTTTTGCCTTTGCCACTGATTGTTGGCATAATTCTTCTGTGTTCTTTTCTACAGTTAAGCTTGGTGTAGGAGGGTTCAGTATGGGAGCGGCTACTGCGCTTTACTCGGCCACCTGCCACACGGTGGGACAATACGGGAATGGAAATCCTTATCCAGCCAATTTGAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCATGTTCAAAGTTAGCTTCTTAATCTTGTCATCTGGACAACCTTTGATCCTTTTGCAATCAATATGAAGTTTCATTTAACAAGTTATCATGGTGATTCCAGGACTTTGAAAACCAAGATGGAAGAGAAGAACGCCAGTAATAGTGGAGCGGGATCCTTGCCTATTTTACTATGCCATGGAAAAGGTATTTAATTTATAAATTTATCATTGTTCTGCAGAAGATGGTTATTTTTCTTACAGATTCTCTGTTCTGTGCTTGGGCTGAAATTCCAGCTTGTTGTGTTGTCCGTATTGCTTATATTTTCACCTTAATTATGGTTAATATGTGTGCTAAGAAGGGATACATTTTGCTCTAATCTTACATAAAAAGATGGGTATCACTGTTAATTTATCTTCGACCGCAGAATTGCATTCGAGCAAAGGAGGAAATAGAGTTTGAGAAAATAGTTTTCTTATGTTGGATTCTGATATTGGTTTGGTGTGAAACTCATTGCAGTTGATGATGTGGTTCTTTATAAATTTGGAGAGAAATCTTCTGAGGCATTACTTTCAAGCGGATTTAAAGACGTTACATTCAAATCTTACAGCGGGTATGTTACGAGAGGCCATAAAGTTCTCGACCTTGTGTGTGTTTCCTTCTGAGGAACTAAGTATTGTACGTGTTTAATATCGACTCATGTTTTGTTTGCAGACTTGGCCACTATACAGTCCCTGAAGAGATGGATGAGGTCTGCTCTTGGTTAACTTCAAAGTTGGGGCTATAGAGGGAGGGGAGGCTCTTCAAATTGCACAATTTTTAACCTTGCTGTTTAGACATTCTTTGACCTACAGTTGATTGGGAACAAATTTGGTGTGAATCTGTGATATTATTATTCCTTTTGGACCTTATATTTGATATGGTTTTTACACCAATATTGAAACCTATTGGTGAACTACAGAGATACTCTGACATGGAATTTGGATAACCTATTCATACACTTGATTGTTACTATTTACAATATCATTCCCTCTATGTTTTGCTTTATGTTATCATTATGATATTTCCCAAGACAGAGATTTGTCTAGAAATTGAAAATTCTGTTTGGTAATCAAAA

mRNA sequence

CATTCATCGAATATTAAAAAAACTCAACCTTTTCCTATGTTTGGGAGTACCCCACCAGCGCTGCAGCTATGGCAAATTAAACACAACAGGTGCGGACCCAGCGGGGCGGGGACACAACACACACACACCCCTCAAAAGAAAACTGTGTGTGAGAGAGAAAGAGAGAGAGAGAGAAACAATCAATACAGCGACGAGAACAACAAGACATAGCCATAAACACACCCACTTTAAACCCCTCTCTCTGGCTTTGGCTGTTCCCCCACCCAGGATCAATTCATAGTCAATCTCTTAAGGTGGTGGTTCACTTGGCTTTTATATTGCGAATATGAGTTTTGCTGGCTCTTCCGTTGCTGCTGGTGGTAGAGCTGCCAAAAAGGCAATTGAGTTTGGTAAAACATACGTAGTGAGGCCCAAGGGAAAACACCAAGCTACCGTTGTCTGGCTTCATGGTCTTGGTGATAATGGCTCAAGCTAGATCGAATGTTTGAACTTTCCCCATTCTGCATTGACTGTACAAGAAATGGAAAGACATTGATTATTCATGTGTCCACTGGCCTTCTGCATTTGCTAGCTCCACTTACTTTCTCAAATGTCATGTTTAGAAATGTTTCAATTTTGTTTGACAGCTGGTCGCAACTATTGGAGACGCTTCCCTTACCAAATATTAAATGGATATGTCCGACTGCGCCTACTCGACCAATAGCTTTATTTGGTGGCTTTCCATCTACTGCATGGTTTGATGTGGAAGATCTTTCAGAAGAAGGTCCGGATGATTTAGAGGGTTTGGATGCTTCAGCTGCACATGTTGCATATTTGTTATCAACAGAGCCAGCTGACATTAAGCTTGGTGTAGGAGGGTTCAGTATGGGAGCGGCTACTGCGCTTTACTCGGCCACCTGCCACACGGTGGGACAATACGGGAATGGAAATCCTTATCCAGCCAATTTGAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCATGTTCAAAGACTTTGAAAACCAAGATGGAAGAGAAGAACGCCAGTAATAGTGGAGCGGGATCCTTGCCTATTTTACTATGCCATGGAAAAGTTGATGATGTGGTTCTTTATAAATTTGGAGAGAAATCTTCTGAGGCATTACTTTCAAGCGGATTTAAAGACGTTACATTCAAATCTTACAGCGGACTTGGCCACTATACAGTCCCTGAAGAGATGGATGAGGTCTGCTCTTGGTTAACTTCAAAGTTGGGGCTATAGAGGGAGGGGAGGCTCTTCAAATTGCACAATTTTTAACCTTGCTGTTTAGACATTCTTTGACCTACAGTTGATTGGGAACAAATTTGGTGTGAATCTGTGATATTATTATTCCTTTTGGACCTTATATTTGATATGGTTTTTACACCAATATTGAAACCTATTGGTGAACTACAGAGATACTCTGACATGGAATTTGGATAACCTATTCATACACTTGATTGTTACTATTTACAATATCATTCCCTCTATGTTTTGCTTTATGTTATCATTATGATATTTCCCAAGACAGAGATTTGTCTAGAAATTGAAAATTCTGTTTGGTAATCAAAA

Coding sequence (CDS)

ATGTTTGAACTTTCCCCATTCTGCATTGACTGTACAAGAAATGGAAAGACATTGATTATTCATGTGTCCACTGGCCTTCTGCATTTGCTAGCTCCACTTACTTTCTCAAATGTCATGTTTAGAAATGTTTCAATTTTGTTTGACAGCTGGTCGCAACTATTGGAGACGCTTCCCTTACCAAATATTAAATGGATATGTCCGACTGCGCCTACTCGACCAATAGCTTTATTTGGTGGCTTTCCATCTACTGCATGGTTTGATGTGGAAGATCTTTCAGAAGAAGGTCCGGATGATTTAGAGGGTTTGGATGCTTCAGCTGCACATGTTGCATATTTGTTATCAACAGAGCCAGCTGACATTAAGCTTGGTGTAGGAGGGTTCAGTATGGGAGCGGCTACTGCGCTTTACTCGGCCACCTGCCACACGGTGGGACAATACGGGAATGGAAATCCTTATCCAGCCAATTTGAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCATGTTCAAAGACTTTGAAAACCAAGATGGAAGAGAAGAACGCCAGTAATAGTGGAGCGGGATCCTTGCCTATTTTACTATGCCATGGAAAAGTTGATGATGTGGTTCTTTATAAATTTGGAGAGAAATCTTCTGAGGCATTACTTTCAAGCGGATTTAAAGACGTTACATTCAAATCTTACAGCGGACTTGGCCACTATACAGTCCCTGAAGAGATGGATGAGGTCTGCTCTTGGTTAACTTCAAAGTTGGGGCTATAG

Protein sequence

MFELSPFCIDCTRNGKTLIIHVSTGLLHLLAPLTFSNVMFRNVSILFDSWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAHVAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPCSKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYSGLGHYTVPEEMDEVCSWLTSKLGL
Homology
BLAST of Lsi06G009420 vs. ExPASy Swiss-Prot
Match: O95372 (Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.2e-25
Identity = 75/209 (35.89%), Postives = 112/209 (53.59%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SW+  L T+ LP++K+ICP AP  P+ L       +WFD+  LS + P+D  G+  +A +
Sbjct: 39  SWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGLSPDAPEDEAGIKKAAEN 98

Query: 109 VAYLLSTE-----PADIKLGVGGFSMGAATALYSA-TCHTVGQYGNGNPYPANLSAVVGL 168
           +  L+  E     PA+ ++ +GGFS G A +LY+A TC          P+P  L+ +V L
Sbjct: 99  IKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTALTC----------PHP--LAGIVAL 158

Query: 169 SGWLPCSKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGF-KD 228
           S WLP  +          A+N  A  L IL CHG++D +V  +FG  ++E L S      
Sbjct: 159 SCWLPLHRAF------PQAANGSAKDLAILQCHGELDPMVPVRFGALTAEKLRSVVTPAR 218

Query: 229 VTFKSYSGLGHYTVPEEMDEVCSWLTSKL 251
           V FK+Y G+ H + P+EM  V  +L   L
Sbjct: 219 VQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of Lsi06G009420 vs. ExPASy Swiss-Prot
Match: Q9WTL7 (Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 5.0e-25
Identity = 74/209 (35.41%), Postives = 112/209 (53.59%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SW+  L T+ LP++K+ICP AP  P+ L       +WFD+  LS + P+D  G+  +A +
Sbjct: 39  SWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGLSPDAPEDEAGIKKAAEN 98

Query: 109 VAYLLSTE-----PADIKLGVGGFSMGAATALYSA-TCHTVGQYGNGNPYPANLSAVVGL 168
           +  L+  E     PA+ ++ +GGFS G A +LY+A TC          P+P  L+ +V L
Sbjct: 99  IKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTALTC----------PHP--LAGIVAL 158

Query: 169 SGWLPCSKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGF-KD 228
           S WLP  +          A+N  A  L IL CHG++D +V  +FG  ++E L +      
Sbjct: 159 SCWLPLHRNF------PQAANGSAKDLAILQCHGELDPMVPVRFGALTAEKLRTVVTPAR 218

Query: 229 VTFKSYSGLGHYTVPEEMDEVCSWLTSKL 251
           V FK+Y G+ H + P+EM  V  +L   L
Sbjct: 219 VQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of Lsi06G009420 vs. ExPASy Swiss-Prot
Match: Q9QYL8 (Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 5.0e-25
Identity = 74/209 (35.41%), Postives = 112/209 (53.59%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SW+  L T+ LP++K+ICP AP  P+ L       +WFD+  LS + P+D  G+  +A +
Sbjct: 39  SWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGLSPDAPEDEAGIKKAAEN 98

Query: 109 VAYLLSTE-----PADIKLGVGGFSMGAATALYSA-TCHTVGQYGNGNPYPANLSAVVGL 168
           +  L+  E     PA+ ++ +GGFS G A +LY+A TC          P+P  L+ +V L
Sbjct: 99  IKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTALTC----------PHP--LAGIVAL 158

Query: 169 SGWLPCSKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGF-KD 228
           S WLP  +          A+N  A  L IL CHG++D +V  +FG  ++E L +      
Sbjct: 159 SCWLPLHRNF------PQAANGSAKDLAILQCHGELDPMVPVRFGALTAEKLRTVVTPAR 218

Query: 229 VTFKSYSGLGHYTVPEEMDEVCSWLTSKL 251
           V FK+Y G+ H + P+EM  V  +L   L
Sbjct: 219 VQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of Lsi06G009420 vs. ExPASy Swiss-Prot
Match: Q4I8Q4 (Acyl-protein thioesterase 1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=FGRAMPH1_01T20223 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 6.3e-20
Identity = 70/243 (28.81%), Postives = 116/243 (47.74%), Query Frame = 0

Query: 30  LAPLTFSNVMFRNVSILF--------DSWSQLLET----LPLPNIKWICPTAPTRPIALF 89
           +APL F        +++F          W+  +E       +  +K+I P AP  PI++ 
Sbjct: 6   IAPLVFPAASQHTATVIFVHGLGDTGHGWASAVENWRRRQKMDEVKFILPHAPQIPISVN 65

Query: 90  GGFPSTAWFDVEDLSEE-----GPDDLEGLDASAAHVAYLLSTE----PADIKLGVGGFS 149
            G     WFD++ L  +       +D EG+  S  +   L+  E        ++ +GGFS
Sbjct: 66  MGMRMPGWFDIKQLGGDVDSLIRNEDTEGIKLSQKYFHNLIQQEIDSGIVPERIVLGGFS 125

Query: 150 MGAATALYSA-TCHTVGQYGNGNPYPANLSAVVGLSGWLPCSKTLKTKMEEKNASNSGAG 209
            G A +L +  TC             + L  ++GLS WL  SKT    ++  +A+     
Sbjct: 126 QGGAMSLLAGLTC------------TSKLGGILGLSSWLLLSKTFADMVKPTDANR---- 185

Query: 210 SLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYSGLGHYTVPEEMDEVCSWLT 251
             P+++ HG+ D +V  + G+ S+E L   G+ DV +K+Y G+GH  VPEE+DEV ++L 
Sbjct: 186 QTPVMMFHGEEDPIVPCERGKLSAELLKGLGY-DVAWKTYPGMGHSAVPEELDEVEAFLR 231

BLAST of Lsi06G009420 vs. ExPASy Swiss-Prot
Match: P0CL95 (Acyl-protein thioesterase 1 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=CNBF2260 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 6.9e-19
Identity = 63/210 (30.00%), Postives = 100/210 (47.62%), Query Frame = 0

Query: 60  PNIKWICPTAPTRPIALFGGFPSTAWFDV---EDLSEEGPDDLEGLDASAAHVAYLLSTE 119
           PN+KWI P APT P++L  G    +WFD+   + L     DD +G+  +   V  L+  E
Sbjct: 45  PNVKWILPHAPTIPVSLNHGMAMPSWFDIRHLDKLDNSENDDEQGMLETLKSVDELIQAE 104

Query: 120 P----ADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPCSKTL 179
                 + ++ +GGFS G A ++            N       L+ VV LS W+P +  +
Sbjct: 105 VDSGIPENRIVLGGFSQGGAISVL-----------NMLTTKRKLAGVVALSTWVPLNHKI 164

Query: 180 KTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSS-GFK----------- 239
              M E       A  +P+   HG  D VV Y+FG++S + L+   G+K           
Sbjct: 165 VQMMSEH------AKDIPVFWGHGTNDPVVDYRFGQRSVDFLVQKCGYKLLSQGTTFARP 224

Query: 240 DVTFKSYSGLGHYTVPEEMDEVCSWLTSKL 251
            + F+SY G+ H + P+E++++ SWL   L
Sbjct: 225 GIRFESYPGMPHSSCPQEIEDLKSWLMEAL 237

BLAST of Lsi06G009420 vs. ExPASy TrEMBL
Match: A0A1S3CMW5 (acyl-protein thioesterase 2 OS=Cucumis melo OX=3656 GN=LOC103502735 PE=4 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 9.5e-112
Identity = 197/204 (96.57%), Postives = 199/204 (97.55%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSE GPDDLEGLDASAAH
Sbjct: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSENGPDDLEGLDASAAH 108

Query: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPC 168
           VAYLLSTEPADIKLGVGGFSMGAATALYSATCH VG+YGNGNPYPANLSAVVGLSGWLPC
Sbjct: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPC 168

Query: 169 SKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228
           SKTLKTKMEEK ASN GAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS
Sbjct: 169 SKTLKTKMEEKKASNIGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228

Query: 229 GLGHYTVPEEMDEVCSWLTSKLGL 253
            LGHYTVPEEMDEVC+WLTSKLGL
Sbjct: 229 SLGHYTVPEEMDEVCAWLTSKLGL 252

BLAST of Lsi06G009420 vs. ExPASy TrEMBL
Match: A0A0A0LU24 (Abhydrolase_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G305760 PE=4 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 6.1e-111
Identity = 194/204 (95.10%), Postives = 199/204 (97.55%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSE+GPDDLEGLDASAAH
Sbjct: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAH 108

Query: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPC 168
           VAYLLSTEPADIKLGVGGFSMGAATALYSATCH VG+YGNGNPYPANLSAVVGLSGWLPC
Sbjct: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPC 168

Query: 169 SKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228
           SKTLKT ME+KNA NSGAGSLPILLCHGKVDDVVLYKFGEKSSEAL SSGFKDVTFKSY+
Sbjct: 169 SKTLKTNMEQKNAGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYN 228

Query: 229 GLGHYTVPEEMDEVCSWLTSKLGL 253
            LGHYTVPEEMDEVC+WLTSKLGL
Sbjct: 229 SLGHYTVPEEMDEVCAWLTSKLGL 252

BLAST of Lsi06G009420 vs. ExPASy TrEMBL
Match: A0A6J1JJZ2 (acyl-protein thioesterase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485265 PE=4 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.9e-108
Identity = 189/204 (92.65%), Postives = 198/204 (97.06%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SWSQLLE LPLPNIKWICPTAPTRPIALFGGFPSTAWFDV+DLSE+GPDDLEGLDASAAH
Sbjct: 49  SWSQLLEMLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVKDLSEDGPDDLEGLDASAAH 108

Query: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPC 168
           VAYLLSTEP+DIKLGVGGFSMGAATALYSATCH VG+YGNGNPYP NLSAVVGLSGWLPC
Sbjct: 109 VAYLLSTEPSDIKLGVGGFSMGAATALYSATCHAVGKYGNGNPYPTNLSAVVGLSGWLPC 168

Query: 169 SKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228
           SKTLK+KMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSS+AL S GF+DV FKSY+
Sbjct: 169 SKTLKSKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSQALHSVGFQDVIFKSYN 228

Query: 229 GLGHYTVPEEMDEVCSWLTSKLGL 253
           GLGHYTVPEEMDEVC+WLTSKLGL
Sbjct: 229 GLGHYTVPEEMDEVCAWLTSKLGL 252

BLAST of Lsi06G009420 vs. ExPASy TrEMBL
Match: A0A6J1EBV8 (acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432778 PE=4 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.9e-107
Identity = 187/204 (91.67%), Postives = 198/204 (97.06%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSE+GPDDLEGLDASAAH
Sbjct: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAH 108

Query: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPC 168
           VAYLLSTEP+DIKL VGGFSMGAATALYSATCH++G+YGNGNPYPANLSAVVGLSGWLPC
Sbjct: 109 VAYLLSTEPSDIKLAVGGFSMGAATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPC 168

Query: 169 SKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228
           SKTLK+KMEE  ASNSGAGSLP+LLCHGKVDDVVLYKFGEKSS+AL S GF+DVTFKSY+
Sbjct: 169 SKTLKSKMEEMKASNSGAGSLPVLLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYN 228

Query: 229 GLGHYTVPEEMDEVCSWLTSKLGL 253
            LGHYTVPEEMDEVC+WLTSKLGL
Sbjct: 229 TLGHYTVPEEMDEVCAWLTSKLGL 252

BLAST of Lsi06G009420 vs. ExPASy TrEMBL
Match: A0A6J1H9A6 (acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461683 PE=4 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 2.4e-107
Identity = 188/204 (92.16%), Postives = 197/204 (96.57%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SWSQLLE LPLPNIKWICPTAPTR IALFGGFPSTAWFDV+DLSE+GPDDLEGLDASAAH
Sbjct: 49  SWSQLLEMLPLPNIKWICPTAPTRSIALFGGFPSTAWFDVKDLSEDGPDDLEGLDASAAH 108

Query: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPC 168
           VAYLLSTEP+DIKLGVGGFSMGAATALYSATCH VG+YGNGNPYP NLSAVVGLSGWLPC
Sbjct: 109 VAYLLSTEPSDIKLGVGGFSMGAATALYSATCHAVGKYGNGNPYPTNLSAVVGLSGWLPC 168

Query: 169 SKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228
           SKTLK+KMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSS+AL S GF+DV FKSY+
Sbjct: 169 SKTLKSKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSQALHSVGFQDVIFKSYN 228

Query: 229 GLGHYTVPEEMDEVCSWLTSKLGL 253
           GLGHYTVPEEMDEVC+WLTSKLGL
Sbjct: 229 GLGHYTVPEEMDEVCAWLTSKLGL 252

BLAST of Lsi06G009420 vs. NCBI nr
Match: XP_008465012.1 (PREDICTED: acyl-protein thioesterase 2 [Cucumis melo] >XP_008465013.1 PREDICTED: acyl-protein thioesterase 2 [Cucumis melo])

HSP 1 Score: 412.9 bits (1060), Expect = 2.0e-111
Identity = 197/204 (96.57%), Postives = 199/204 (97.55%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSE GPDDLEGLDASAAH
Sbjct: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSENGPDDLEGLDASAAH 108

Query: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPC 168
           VAYLLSTEPADIKLGVGGFSMGAATALYSATCH VG+YGNGNPYPANLSAVVGLSGWLPC
Sbjct: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPC 168

Query: 169 SKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228
           SKTLKTKMEEK ASN GAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS
Sbjct: 169 SKTLKTKMEEKKASNIGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228

Query: 229 GLGHYTVPEEMDEVCSWLTSKLGL 253
            LGHYTVPEEMDEVC+WLTSKLGL
Sbjct: 229 SLGHYTVPEEMDEVCAWLTSKLGL 252

BLAST of Lsi06G009420 vs. NCBI nr
Match: XP_038879397.1 (acyl-protein thioesterase 2 [Benincasa hispida])

HSP 1 Score: 412.1 bits (1058), Expect = 3.3e-111
Identity = 195/204 (95.59%), Postives = 200/204 (98.04%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSE+GPDDLEGLDASAAH
Sbjct: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAH 108

Query: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPC 168
           VAYLLSTEPADIKLGVGGFSMGAATALYSATCH VG+YGNGNPYPANLSAVVGLSGWLPC
Sbjct: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPC 168

Query: 169 SKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228
           SKTLKTKMEEKNA+NSG GSLPILLCHGKVDDVVLYKFGEKSSEAL S GFKDVTFKSYS
Sbjct: 169 SKTLKTKMEEKNANNSGVGSLPILLCHGKVDDVVLYKFGEKSSEALHSCGFKDVTFKSYS 228

Query: 229 GLGHYTVPEEMDEVCSWLTSKLGL 253
           G+GHYTVPEEMDEVC+WLTSKLGL
Sbjct: 229 GVGHYTVPEEMDEVCAWLTSKLGL 252

BLAST of Lsi06G009420 vs. NCBI nr
Match: XP_004150012.1 (acyl-protein thioesterase 2 [Cucumis sativus] >XP_031736139.1 acyl-protein thioesterase 2 [Cucumis sativus] >KGN65303.1 hypothetical protein Csa_019754 [Cucumis sativus])

HSP 1 Score: 410.2 bits (1053), Expect = 1.3e-110
Identity = 194/204 (95.10%), Postives = 199/204 (97.55%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSE+GPDDLEGLDASAAH
Sbjct: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAH 108

Query: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPC 168
           VAYLLSTEPADIKLGVGGFSMGAATALYSATCH VG+YGNGNPYPANLSAVVGLSGWLPC
Sbjct: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPC 168

Query: 169 SKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228
           SKTLKT ME+KNA NSGAGSLPILLCHGKVDDVVLYKFGEKSSEAL SSGFKDVTFKSY+
Sbjct: 169 SKTLKTNMEQKNAGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYN 228

Query: 229 GLGHYTVPEEMDEVCSWLTSKLGL 253
            LGHYTVPEEMDEVC+WLTSKLGL
Sbjct: 229 SLGHYTVPEEMDEVCAWLTSKLGL 252

BLAST of Lsi06G009420 vs. NCBI nr
Match: XP_022987844.1 (acyl-protein thioesterase 2-like [Cucurbita maxima] >XP_022987845.1 acyl-protein thioesterase 2-like [Cucurbita maxima])

HSP 1 Score: 401.4 bits (1030), Expect = 5.9e-108
Identity = 189/204 (92.65%), Postives = 198/204 (97.06%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SWSQLLE LPLPNIKWICPTAPTRPIALFGGFPSTAWFDV+DLSE+GPDDLEGLDASAAH
Sbjct: 49  SWSQLLEMLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVKDLSEDGPDDLEGLDASAAH 108

Query: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPC 168
           VAYLLSTEP+DIKLGVGGFSMGAATALYSATCH VG+YGNGNPYP NLSAVVGLSGWLPC
Sbjct: 109 VAYLLSTEPSDIKLGVGGFSMGAATALYSATCHAVGKYGNGNPYPTNLSAVVGLSGWLPC 168

Query: 169 SKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228
           SKTLK+KMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSS+AL S GF+DV FKSY+
Sbjct: 169 SKTLKSKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSQALHSVGFQDVIFKSYN 228

Query: 229 GLGHYTVPEEMDEVCSWLTSKLGL 253
           GLGHYTVPEEMDEVC+WLTSKLGL
Sbjct: 229 GLGHYTVPEEMDEVCAWLTSKLGL 252

BLAST of Lsi06G009420 vs. NCBI nr
Match: XP_023515795.1 (acyl-protein thioesterase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 401.0 bits (1029), Expect = 7.7e-108
Identity = 190/204 (93.14%), Postives = 197/204 (96.57%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SWSQLLE LPLPNIKWICPTAPTRPIALFGGFPSTAWFDV+DLSE GPDDLEGLDASAAH
Sbjct: 49  SWSQLLEMLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVKDLSEGGPDDLEGLDASAAH 108

Query: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPC 168
           VAYLLSTEPADIKLGVGGFSMGAATALYSATCH VG+YGNGNPYP NLSAVVGLSGWLPC
Sbjct: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNPYPTNLSAVVGLSGWLPC 168

Query: 169 SKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228
           SKTLK+KMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSS+AL S GF+DV FKSY+
Sbjct: 169 SKTLKSKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSQALHSVGFQDVIFKSYN 228

Query: 229 GLGHYTVPEEMDEVCSWLTSKLGL 253
           GLGHYTVPEEMDEVC+WLTSKLGL
Sbjct: 229 GLGHYTVPEEMDEVCAWLTSKLGL 252

BLAST of Lsi06G009420 vs. TAIR 10
Match: AT5G20060.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 340.9 bits (873), Expect = 8.8e-94
Identity = 155/204 (75.98%), Postives = 182/204 (89.22%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SWSQLLETLPLPNIKWICPTAP++PI+LFGGFPSTAWFDV D++E+GPDD+EGLD +AAH
Sbjct: 48  SWSQLLETLPLPNIKWICPTAPSQPISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAH 107

Query: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPC 168
           VA LLS EPADIKLGVGGFSMGAAT+LYSATC  +G+YGNGNPYP NLSA++GLSGWLPC
Sbjct: 108 VANLLSNEPADIKLGVGGFSMGAATSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPC 167

Query: 169 SKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228
           +KTL  K+EE+   N  A SLPI++CHGK DDVV +KFGEKSS+ALLS+GFK VTFK YS
Sbjct: 168 AKTLAGKLEEEQIKNR-AASLPIVVCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYS 227

Query: 229 GLGHYTVPEEMDEVCSWLTSKLGL 253
            LGH+T+P+E+DE+C+WLTS L L
Sbjct: 228 ALGHHTIPQELDELCAWLTSTLSL 250

BLAST of Lsi06G009420 vs. TAIR 10
Match: AT5G20060.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 340.9 bits (873), Expect = 8.8e-94
Identity = 155/204 (75.98%), Postives = 182/204 (89.22%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SWSQLLETLPLPNIKWICPTAP++PI+LFGGFPSTAWFDV D++E+GPDD+EGLD +AAH
Sbjct: 48  SWSQLLETLPLPNIKWICPTAPSQPISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAH 107

Query: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPC 168
           VA LLS EPADIKLGVGGFSMGAAT+LYSATC  +G+YGNGNPYP NLSA++GLSGWLPC
Sbjct: 108 VANLLSNEPADIKLGVGGFSMGAATSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPC 167

Query: 169 SKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228
           +KTL  K+EE+   N  A SLPI++CHGK DDVV +KFGEKSS+ALLS+GFK VTFK YS
Sbjct: 168 AKTLAGKLEEEQIKNR-AASLPIVVCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYS 227

Query: 229 GLGHYTVPEEMDEVCSWLTSKLGL 253
            LGH+T+P+E+DE+C+WLTS L L
Sbjct: 228 ALGHHTIPQELDELCAWLTSTLSL 250

BLAST of Lsi06G009420 vs. TAIR 10
Match: AT5G20060.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 340.9 bits (873), Expect = 8.8e-94
Identity = 155/204 (75.98%), Postives = 182/204 (89.22%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           SWSQLLETLPLPNIKWICPTAP++PI+LFGGFPSTAWFDV D++E+GPDD+EGLD +AAH
Sbjct: 48  SWSQLLETLPLPNIKWICPTAPSQPISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAH 107

Query: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPC 168
           VA LLS EPADIKLGVGGFSMGAAT+LYSATC  +G+YGNGNPYP NLSA++GLSGWLPC
Sbjct: 108 VANLLSNEPADIKLGVGGFSMGAATSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPC 167

Query: 169 SKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228
           +KTL  K+EE+   N  A SLPI++CHGK DDVV +KFGEKSS+ALLS+GFK VTFK YS
Sbjct: 168 AKTLAGKLEEEQIKNR-AASLPIVVCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYS 227

Query: 229 GLGHYTVPEEMDEVCSWLTSKLGL 253
            LGH+T+P+E+DE+C+WLTS L L
Sbjct: 228 ALGHHTIPQELDELCAWLTSTLSL 250

BLAST of Lsi06G009420 vs. TAIR 10
Match: AT3G15650.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 4.0e-78
Identity = 134/209 (64.11%), Postives = 164/209 (78.47%), Query Frame = 0

Query: 44  SILFDSWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLD 103
           S+ F S SQLLE+LPLPNIKWICPTAP+RP++L GGFP TAWFDV ++SE+  DD+EGLD
Sbjct: 62  SVSFCSSSQLLESLPLPNIKWICPTAPSRPVSLLGGFPCTAWFDVGEISEDLHDDIEGLD 121

Query: 104 ASAAHVAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLS 163
           ASAAH+A LLS EP D+K+G+GGFSMGAA ALYS TC+ +G+YG G+ Y  NL A VGLS
Sbjct: 122 ASAAHIANLLSAEPTDVKVGIGGFSMGAAIALYSTTCYALGRYGTGHAYTINLRATVGLS 181

Query: 164 GWLPCSKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVT 223
           GWLP  ++L++K+E  N     A S+PILL HG  DDVV Y+FGEKS+ +L  +GF+   
Sbjct: 182 GWLPGWRSLRSKIESSNEVARRAASIPILLAHGTSDDVVPYRFGEKSAHSLAMAGFRQTM 241

Query: 224 FKSYSGLGHYTVPEEMDEVCSWLTSKLGL 253
           FK Y GLGHYTVP+EMDEV  WL S+LGL
Sbjct: 242 FKPYEGLGHYTVPKEMDEVVHWLVSRLGL 270

BLAST of Lsi06G009420 vs. TAIR 10
Match: AT3G15650.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 286.2 bits (731), Expect = 2.6e-77
Identity = 132/204 (64.71%), Postives = 161/204 (78.92%), Query Frame = 0

Query: 49  SWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEEGPDDLEGLDASAAH 108
           S SQLLE+LPLPNIKWICPTAP+RP++L GGFP TAWFDV ++SE+  DD+EGLDASAAH
Sbjct: 48  SSSQLLESLPLPNIKWICPTAPSRPVSLLGGFPCTAWFDVGEISEDLHDDIEGLDASAAH 107

Query: 109 VAYLLSTEPADIKLGVGGFSMGAATALYSATCHTVGQYGNGNPYPANLSAVVGLSGWLPC 168
           +A LLS EP D+K+G+GGFSMGAA ALYS TC+ +G+YG G+ Y  NL A VGLSGWLP 
Sbjct: 108 IANLLSAEPTDVKVGIGGFSMGAAIALYSTTCYALGRYGTGHAYTINLRATVGLSGWLPG 167

Query: 169 SKTLKTKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYS 228
            ++L++K+E  N     A S+PILL HG  DDVV Y+FGEKS+ +L  +GF+   FK Y 
Sbjct: 168 WRSLRSKIESSNEVARRAASIPILLAHGTSDDVVPYRFGEKSAHSLAMAGFRQTMFKPYE 227

Query: 229 GLGHYTVPEEMDEVCSWLTSKLGL 253
           GLGHYTVP+EMDEV  WL S+LGL
Sbjct: 228 GLGHYTVPKEMDEVVHWLVSRLGL 251

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O953722.2e-2535.89Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1[more]
Q9WTL75.0e-2535.41Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1[more]
Q9QYL85.0e-2535.41Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1[more]
Q4I8Q46.3e-2028.81Acyl-protein thioesterase 1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 12365... [more]
P0CL956.9e-1930.00Acyl-protein thioesterase 1 OS=Cryptococcus neoformans var. neoformans serotype ... [more]
Match NameE-valueIdentityDescription
A0A1S3CMW59.5e-11296.57acyl-protein thioesterase 2 OS=Cucumis melo OX=3656 GN=LOC103502735 PE=4 SV=1[more]
A0A0A0LU246.1e-11195.10Abhydrolase_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G3057... [more]
A0A6J1JJZ22.9e-10892.65acyl-protein thioesterase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485265 PE=... [more]
A0A6J1EBV81.9e-10791.67acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432778 P... [more]
A0A6J1H9A62.4e-10792.16acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461683 P... [more]
Match NameE-valueIdentityDescription
XP_008465012.12.0e-11196.57PREDICTED: acyl-protein thioesterase 2 [Cucumis melo] >XP_008465013.1 PREDICTED:... [more]
XP_038879397.13.3e-11195.59acyl-protein thioesterase 2 [Benincasa hispida][more]
XP_004150012.11.3e-11095.10acyl-protein thioesterase 2 [Cucumis sativus] >XP_031736139.1 acyl-protein thioe... [more]
XP_022987844.15.9e-10892.65acyl-protein thioesterase 2-like [Cucurbita maxima] >XP_022987845.1 acyl-protein... [more]
XP_023515795.17.7e-10893.14acyl-protein thioesterase 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G20060.28.8e-9475.98alpha/beta-Hydrolases superfamily protein [more]
AT5G20060.18.8e-9475.98alpha/beta-Hydrolases superfamily protein [more]
AT5G20060.38.8e-9475.98alpha/beta-Hydrolases superfamily protein [more]
AT3G15650.24.0e-7864.11alpha/beta-Hydrolases superfamily protein [more]
AT3G15650.12.6e-7764.71alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003140Phospholipase/carboxylesterase/thioesterasePFAMPF02230Abhydrolase_2coord: 49..246
e-value: 5.7E-28
score: 98.2
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 47..251
e-value: 1.8E-49
score: 170.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 48..250
NoneNo IPR availablePANTHERPTHR46234:SF9ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 49..252
NoneNo IPR availablePANTHERPTHR46234ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 49..252

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G009420.1Lsi06G009420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity