Lsi06G009380 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G009380
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCytochrome P450
Locationchr06: 19296181 .. 19298429 (+)
RNA-Seq ExpressionLsi06G009380
SyntenyLsi06G009380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGGTGTTTGTGGGTTTTGTTTATTTAAAAGCCAGAAGAGAAGCAGCGTTTTAGTAATTCTATACCCAAAAAAAAAAACCCCATTTCAAAAGAAAAGAGAGAATGAAGATTGAATCAATATTGATGAAGGTGCCACCGGTATGGGCGGCGGCGGTGACGGCGGCGGTGGTGATAATTTTAAGTGCAAAAATGGCTTTAATTTTATGGTGGAATCCACGAAGAATAGAAAAGCATTTTCATAGACAAGGAATTCGAGGCCCTCCTTATCGTTTCTTCATTGGTAATTTTAAAGAACTTGTTTCTATGATGCTTGCTGCTTCTTCTCATCCAATGCCCAATTTCTCTCATAATATTCTCCCTCGTGTCCTCTCTTTTTACCATCATTGGAAGAAAATTTACGGTACGTATCAAATTTTTATTCATTATATTCCCTTTTTTTTTCATTTCATTTTCTAATTAACATCATCCGTTTGGATTGACTTTCTAGGCTCGACTTTTTTAATTTGGTTCGGACCCATTGTTCGAGTCGCCGTTTCCGACCCGGACATGATCCGAGAAATCTTCACCACTAATTCTCACTTCTACGAGAAGAATGAAGCTCATCCTCTTGTCAAACAGCTCGAAGGCGATGGCCTCCTCACTCTCAAAGGCCAAAAATGGGCTCTCCATCGTAAAATCATCTCCCCCACTTTCCATATGAATAATCTCAAAGTAAACTCTAAACCGTGTTTTTTTTTAAAATATGAAAATATACCTTAACTAGTTCAACTAAATTCAAATGGGTTGCATGGATTTTTAGTTGTTGATTCCGTTGGTGGAGGAAAGTGTGGTGGAAATGGTGGAGAAATGGTCGTCTATGGCGGAGAGTGTCGATTCCGGGGAAATCGAGATCGATGTTTTCGAGTGCTTTCAAACCCTAACGGAAGATGTTATTACAAAAACAGCATTTGGTAGTAGCTACGAAGATGGTAAAGCTATATTTAAGCTTCAAACTCAACAAATGATATTAGCTTCTCAAGCTTTTCAAAAGGTCTTCATTCCTGGCTATAGGTAAAATTACATCTTAATTAATTCAATATTTTTTTATTCTTTCTAATTTTGGGGTTTTTTTTTTATTAATTGATTTTTTTTTTTTTTTTTTTTAAGATTTCTACCGACAAGAACGAATATAAATTCGTGGAAATTAGACAAGCAAATAAGGAAATCATTGATGAAATTAATCGAGAGTCGAAAAGGGAATTCAGAAAAAATTTGGGCAATGGATGAAAAAAAGCCAAAAGATTTGTTAGGGCTGATGATTCAAGCTTCCTCCTTAAAAAATAATAATTCCATTACTGTCCATGACATCGTTGAAGAATGCAAAAGCTTCTTCTTTGCTGGCAAACAAACCACTTCCAATCTGCTGACGTGGACTACTGTCTTGCTGGCAATGCATCCTCAGTGGCAAATTCAGGCACGTGATGAGGTCTTTAACGTCTGCGGTTCACGTGCCCTCCCTTCCAAAGATGATGTCTCCAACTTCAAAATCGTAAGCCCTTTTTTTTTTTTTCTCTTTTTCTATTTATATTTCATTATTTAAAAAAATGTTAATAATAATAAAAAAAAAAAAAAAATTGTTTTGTCATTTTACTAATAAAAACTAATAAACTGCCAACTTTTGGTTTACGTTGCTTGGTTTGATTAATGGAAAAATTTTGTGTGTTTGGTGGGGAAATGATTGGGTGTGGCAGCTGAGCATGATACTAAATGAATCCTTACGGTTATACCCACCCACGGTGGCAACAATCCGACGTGCAAAGGCTGACGTGGAGCTAGGTGGACGAATGATCCCACGTGGAACGGAGTTTTTAATTCCAATCCTAGCCGTTCATCATGATCAAAGTATTTGGGGCAACGATGCGAGCGAGTTCAACCCAGGGCGGTTCTCAGAAGGAGTGGGGCGAGCCGGAAATCACCCAGCCGGGTTTATTCCATTTGGACTTGGAGTACGTACTTGCATTGGCCAAAATCTGGCAGTTTTGGAAGCAAAATTAACAATGGCAATTATACTTCAACGGTTCACGTTTCGTTTGGCTCCCACTTATCAACATGCACCAACGGTCCTGATGCTTCTTTATCCACAATATGGGGCCCCCGTTCTTTTTCAAAAGATACCCTTAAATTAAAAACTCCTCATTTATATATATGGCTAATTTTAATTAACCCATATAATTATTATTATTCTCTATTAGTATAATTAAGATCATCA

mRNA sequence

GTTGGTGTTTGTGGGTTTTGTTTATTTAAAAGCCAGAAGAGAAGCAGCGTTTTAGTAATTCTATACCCAAAAAAAAAAACCCCATTTCAAAAGAAAAGAGAGAATGAAGATTGAATCAATATTGATGAAGGTGCCACCGGTATGGGCGGCGGCGGTGACGGCGGCGGTGGTGATAATTTTAAGTGCAAAAATGGCTTTAATTTTATGGTGGAATCCACGAAGAATAGAAAAGCATTTTCATAGACAAGGAATTCGAGGCCCTCCTTATCGTTTCTTCATTGGTAATTTTAAAGAACTTGTTTCTATGATGCTTGCTGCTTCTTCTCATCCAATGCCCAATTTCTCTCATAATATTCTCCCTCGTGTCCTCTCTTTTTACCATCATTGGAAGAAAATTTACGGCTCGACTTTTTTAATTTGGTTCGGACCCATTGTTCGAGTCGCCGTTTCCGACCCGGACATGATCCGAGAAATCTTCACCACTAATTCTCACTTCTACGAGAAGAATGAAGCTCATCCTCTTGTCAAACAGCTCGAAGGCGATGGCCTCCTCACTCTCAAAGGCCAAAAATGGGCTCTCCATCGTAAAATCATCTCCCCCACTTTCCATATGAATAATCTCAAATTGTTGATTCCGTTGGTGGAGGAAAGTGTGGTGGAAATGGTGGAGAAATGGTCGTCTATGGCGGAGAGTGTCGATTCCGGGGAAATCGAGATCGATGTTTTCGAGTGCTTTCAAACCCTAACGGAAGATGTTATTACAAAAACAGCATTTGGTAGTAGCTACGAAGATGGTAAAGCTATATTTAAGCTTCAAACTCAACAAATGATATTAGCTTCTCAAGCTTTTCAAAAGGTCTTCATTCCTGGCTATAGATTTCTACCGACAAGAACGAATATAAATTCGTGGAAATTAGACAAGCAAATAAGGAAATCATTGATGAAATTAATCGAGAGTCGAAAAGGGAATTCAGAAAAAATTTGGGCAATGGATGAAAAAAAGCCAAAAGATTTGTTAGGGCTGATGATTCAAGCTTCCTCCTTAAAAAATAATAATTCCATTACTGTCCATGACATCGTTGAAGAATGCAAAAGCTTCTTCTTTGCTGGCAAACAAACCACTTCCAATCTGCTGACGTGGACTACTGTCTTGCTGGCAATGCATCCTCAGTGGCAAATTCAGGCACGTGATGAGGTCTTTAACGTCTGCGGTTCACGTGCCCTCCCTTCCAAAGATGATGTCTCCAACTTCAAAATCCTGAGCATGATACTAAATGAATCCTTACGGTTATACCCACCCACGGTGGCAACAATCCGACGTGCAAAGGCTGACGTGGAGCTAGGTGGACGAATGATCCCACGTGGAACGGAGTTTTTAATTCCAATCCTAGCCGTTCATCATGATCAAAGTATTTGGGGCAACGATGCGAGCGAGTTCAACCCAGGGCGGTTCTCAGAAGGAGTGGGGCGAGCCGGAAATCACCCAGCCGGGTTTATTCCATTTGGACTTGGAGTACGTACTTGCATTGGCCAAAATCTGGCAGTTTTGGAAGCAAAATTAACAATGGCAATTATACTTCAACGGTTCACGTTTCGTTTGGCTCCCACTTATCAACATGCACCAACGGTCCTGATGCTTCTTTATCCACAATATGGGGCCCCCGTTCTTTTTCAAAAGATACCCTTAAATTAAAAACTCCTCATTTATATATATGGCTAATTTTAATTAACCCATATAATTATTATTATTCTCTATTAGTATAATTAAGATCATCA

Coding sequence (CDS)

ATGAAGATTGAATCAATATTGATGAAGGTGCCACCGGTATGGGCGGCGGCGGTGACGGCGGCGGTGGTGATAATTTTAAGTGCAAAAATGGCTTTAATTTTATGGTGGAATCCACGAAGAATAGAAAAGCATTTTCATAGACAAGGAATTCGAGGCCCTCCTTATCGTTTCTTCATTGGTAATTTTAAAGAACTTGTTTCTATGATGCTTGCTGCTTCTTCTCATCCAATGCCCAATTTCTCTCATAATATTCTCCCTCGTGTCCTCTCTTTTTACCATCATTGGAAGAAAATTTACGGCTCGACTTTTTTAATTTGGTTCGGACCCATTGTTCGAGTCGCCGTTTCCGACCCGGACATGATCCGAGAAATCTTCACCACTAATTCTCACTTCTACGAGAAGAATGAAGCTCATCCTCTTGTCAAACAGCTCGAAGGCGATGGCCTCCTCACTCTCAAAGGCCAAAAATGGGCTCTCCATCGTAAAATCATCTCCCCCACTTTCCATATGAATAATCTCAAATTGTTGATTCCGTTGGTGGAGGAAAGTGTGGTGGAAATGGTGGAGAAATGGTCGTCTATGGCGGAGAGTGTCGATTCCGGGGAAATCGAGATCGATGTTTTCGAGTGCTTTCAAACCCTAACGGAAGATGTTATTACAAAAACAGCATTTGGTAGTAGCTACGAAGATGGTAAAGCTATATTTAAGCTTCAAACTCAACAAATGATATTAGCTTCTCAAGCTTTTCAAAAGGTCTTCATTCCTGGCTATAGATTTCTACCGACAAGAACGAATATAAATTCGTGGAAATTAGACAAGCAAATAAGGAAATCATTGATGAAATTAATCGAGAGTCGAAAAGGGAATTCAGAAAAAATTTGGGCAATGGATGAAAAAAAGCCAAAAGATTTGTTAGGGCTGATGATTCAAGCTTCCTCCTTAAAAAATAATAATTCCATTACTGTCCATGACATCGTTGAAGAATGCAAAAGCTTCTTCTTTGCTGGCAAACAAACCACTTCCAATCTGCTGACGTGGACTACTGTCTTGCTGGCAATGCATCCTCAGTGGCAAATTCAGGCACGTGATGAGGTCTTTAACGTCTGCGGTTCACGTGCCCTCCCTTCCAAAGATGATGTCTCCAACTTCAAAATCCTGAGCATGATACTAAATGAATCCTTACGGTTATACCCACCCACGGTGGCAACAATCCGACGTGCAAAGGCTGACGTGGAGCTAGGTGGACGAATGATCCCACGTGGAACGGAGTTTTTAATTCCAATCCTAGCCGTTCATCATGATCAAAGTATTTGGGGCAACGATGCGAGCGAGTTCAACCCAGGGCGGTTCTCAGAAGGAGTGGGGCGAGCCGGAAATCACCCAGCCGGGTTTATTCCATTTGGACTTGGAGTACGTACTTGCATTGGCCAAAATCTGGCAGTTTTGGAAGCAAAATTAACAATGGCAATTATACTTCAACGGTTCACGTTTCGTTTGGCTCCCACTTATCAACATGCACCAACGGTCCTGATGCTTCTTTATCCACAATATGGGGCCCCCGTTCTTTTTCAAAAGATACCCTTAAATTAA

Protein sequence

MKIESILMKVPPVWAAAVTAAVVIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRFFIGNFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLVEESVVEMVEKWSSMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSEKIWAMDEKKPKDLLGLMIQASSLKNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVFNVCGSRALPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLAVLEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKIPLN
Homology
BLAST of Lsi06G009380 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 1.7e-216
Identity = 370/528 (70.08%), Postives = 440/528 (83.33%), Query Frame = 0

Query: 1   MKIESILMKVPPVWAAAVTAAVVIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRFFIG 60
           M+ ES    +P V   +V  ++VI+   K   +LWW PR+IE+HF +QGIRGPPY FFIG
Sbjct: 1   MEEESSSWFIPKVLVLSVILSLVIV---KGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIG 60

Query: 61  NFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM 120
           N KELV MML ASSHPMP FSHNILPRVLSFYHHW+KIYG+TFL+WFGP  R+ V+DPD+
Sbjct: 61  NVKELVGMMLKASSHPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDL 120

Query: 121 IREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLV 180
           IREIF + S FYEKNEAHPLVKQLEGDGLL+LKG+KWA HRKIISPTFHM NLKLL+P+V
Sbjct: 121 IREIF-SKSEFYEKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVV 180

Query: 181 EESVVEMVEKWSSMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ 240
            +SV +MV+KWS      ++GE+E+DV+E FQ LTEDVI++TAFGSSYEDG+A+F+LQ Q
Sbjct: 181 LKSVTDMVDKWSDKLS--ENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQ 240

Query: 241 QMILASQAFQKVFIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSEKIWAMDEKK 300
           QM+L ++AFQKVFIPGYRF PTR N+ SWKLDK+IRKSL+KLIE R+ N+      + K+
Sbjct: 241 QMLLCAEAFQKVFIPGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKE 300

Query: 301 P--KDLLGLMIQASSLKNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQ 360
           P  KDLLGLMIQA       ++TV DIVEECKSFFFAGKQTTSNLLTWTT+LL+MHP+WQ
Sbjct: 301 PAAKDLLGLMIQA------KNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQ 360

Query: 361 IQARDEVFNVCGSRALPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELGGRMI 420
            +ARDEV  VCGSR +P+KD V   K LSMILNESLRLYPP VATIRRAK+DV+LGG  I
Sbjct: 361 AKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKI 420

Query: 421 PRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQN 480
           P GTE LIPI+AVHHDQ+IWGND +EFNP RF++GV RA  HP GFIPFGLGVRTCIGQN
Sbjct: 421 PCGTELLIPIIAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQN 480

Query: 481 LAVLEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKI 527
           LA+L+AKLT+A+++QRFTF LAPTYQHAPTVLMLLYPQ+GAP+ F+++
Sbjct: 481 LAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRL 515

BLAST of Lsi06G009380 vs. ExPASy Swiss-Prot
Match: B9X287 (Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 4.6e-190
Identity = 321/524 (61.26%), Postives = 413/524 (78.82%), Query Frame = 0

Query: 14  WAAAVTAAVVIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRFFIGNFKELVSMMLAAS 73
           WAAA  AA   + + K+  +LWW PRR+E+HF RQGI GP YRF +G  +E+V++M+AAS
Sbjct: 6   WAAAAAAAAAWV-AVKVLEVLWWRPRRVEEHFARQGITGPRYRFLVGCVREMVALMVAAS 65

Query: 74  SHPM--PNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHF 133
           + PM  P  SHN+LPRVL+FYHHWKKIYGSTFLIWFGP  R+A++DP++IRE+    +  
Sbjct: 66  AKPMPPPYRSHNVLPRVLAFYHHWKKIYGSTFLIWFGPTPRLAIADPELIREVLLARADR 125

Query: 134 YEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLVEESVVEMVEKW 193
           +++ E+HP+V+QLEG+GL++L+G KWA HR++++P FHM+NL+LL+P V  +V++M +KW
Sbjct: 126 FDRYESHPMVRQLEGEGLVSLRGDKWAHHRRVLTPAFHMDNLRLLLPCVGMTVLDMADKW 185

Query: 194 SSMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQK 253
            +MAE+  SGE+EIDV + FQ +TED IT+TAFG SYEDGK +FKLQ Q M  AS+AF+K
Sbjct: 186 RAMAEADKSGEVEIDVSDWFQVVTEDAITRTAFGRSYEDGKVVFKLQAQLMAFASEAFRK 245

Query: 254 VFIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRK--GNSEKIWAMDEKKPKDLLGLMI 313
           VFIPGYRFLPT+ N +SWKLDK+IRK+L+ LI  R+  G+ EK+    +   KDLLGLMI
Sbjct: 246 VFIPGYRFLPTKKNTSSWKLDKEIRKNLVTLIGRRQEAGDDEKL----DGCAKDLLGLMI 305

Query: 314 QASSLKNN--------NSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQA 373
            A++  N         + ITV+DIVEECK+FFFAGKQTTSNLLTW  V+LAMHP+WQ +A
Sbjct: 306 NAAASSNGGKRSALPVSPITVNDIVEECKTFFFAGKQTTSNLLTWAIVVLAMHPEWQERA 365

Query: 374 RDEVFNVCGSRALPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELGGRM-IPR 433
           R EV +VCG+  +PS++ ++  K L MILNE+LRLYPP VAT+RRAKADVELGG + IPR
Sbjct: 366 RQEVLDVCGADGVPSREQLAKLKTLGMILNETLRLYPPAVATVRRAKADVELGGYLRIPR 425

Query: 434 GTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLA 493
            TE LIPI+AVHHD  +WG DA++FNP RF+ GV RA  HPA FIPFGLG R CIGQNLA
Sbjct: 426 DTELLIPIMAVHHDARLWGPDAAQFNPARFAGGVARAARHPAAFIPFGLGARMCIGQNLA 485

Query: 494 VLEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQ 525
           +LEAKLT+A+IL RF FRL+  Y HAPTVLMLL+PQYGAP++F+
Sbjct: 486 ILEAKLTVAVILHRFEFRLSARYVHAPTVLMLLHPQYGAPIVFR 524

BLAST of Lsi06G009380 vs. ExPASy Swiss-Prot
Match: Q6Z6D6 (Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 1.5e-177
Identity = 303/526 (57.60%), Postives = 391/526 (74.33%), Query Frame = 0

Query: 15  AAAVTAAVVIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRFFIGNFKELVSMMLAASS 74
           AAA    V + ++A+MA  LWW PRR+E HF  QG+RGPPYRF +G+ +E+V++M  ASS
Sbjct: 22  AAAAAVWVTMHVAARMADALWWRPRRLEAHFAAQGVRGPPYRFLLGSVREMVALMAEASS 81

Query: 75  HPM-PNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFYE 134
            PM P  SHN LPRVL+FYH+W+KIYG  FLIWFGP  R+ V++P++IREIF T +  ++
Sbjct: 82  KPMSPPTSHNALPRVLAFYHYWRKIYGHRFLIWFGPTPRLTVAEPELIREIFLTRADAFD 141

Query: 135 KNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLVEESVVEMVEKWSS 194
           + EAHP+V+QLEGDGL++L G KWALHR++++  F+ +NL  LIP V +SV  +  KW +
Sbjct: 142 RYEAHPVVRQLEGDGLVSLHGDKWALHRRVLTDAFYPDNLNRLIPHVGKSVAALAAKWGA 201

Query: 195 MAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQKVF 254
           MAE+  SGE+E+DV E FQ +TE+ IT+  FG SY+DG+ +F +Q Q M  AS+AF+KV 
Sbjct: 202 MAEAGGSGEVEVDVAEWFQAVTEEAITRATFGRSYDDGRVVFAMQGQLMAFASEAFRKVL 261

Query: 255 IPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSEKIWAMDEKKPKDLLGLMIQASS 314
           +PGYRFLPT+ N  SW+LD++IR+SLM+LI  R   +E+    D+   +DLLGLMI A +
Sbjct: 262 VPGYRFLPTKKNRLSWRLDREIRRSLMRLIGRRSDEAEQGEKADDGSFRDLLGLMINAGA 321

Query: 315 L----------KNN--NSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQA 374
                      KN+   +I V D++EECK+FFFAGKQTT+NLLTW TVLLAMHP WQ +A
Sbjct: 322 AAATRGNAGGEKNSPAAAIPVEDMLEECKTFFFAGKQTTTNLLTWATVLLAMHPDWQERA 381

Query: 375 RDEVFNVCGSRALPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELG-GRMIPR 434
           R EVF+VCG+  LPSK+ +   K L MI+NE+LRLYPP VATIRRAK DV+L  G MIPR
Sbjct: 382 RREVFDVCGAGELPSKEHLPKLKTLGMIMNETLRLYPPAVATIRRAKVDVQLSDGCMIPR 441

Query: 435 GTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLA 494
             E L+PI+A+HHD   WG DAS+FNP RF+ G  +A  HP  FIPFGLG R C+GQNLA
Sbjct: 442 DMELLVPIMAIHHDTRYWGPDASQFNPARFANGASKAAKHPLAFIPFGLGSRMCVGQNLA 501

Query: 495 VLEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKI 527
            LEAKLTMAI+LQRF  R +P Y HAPTVLMLLYPQYGAP++F+ +
Sbjct: 502 RLEAKLTMAILLQRFEIRTSPNYVHAPTVLMLLYPQYGAPLIFRPL 547

BLAST of Lsi06G009380 vs. ExPASy Swiss-Prot
Match: Q69XM6 (Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 9.0e-170
Identity = 297/531 (55.93%), Postives = 386/531 (72.69%), Query Frame = 0

Query: 15  AAAVTAAVVIIL--SAKMALILWWNPRRIEKHFHRQGIRGPPYRFFIGNFKELVSMMLAA 74
           A AV AAV+++L  +A++A  +WW PRR+E HF  QG+RGPPYRF +G  +E+V++M  A
Sbjct: 4   AVAVAAAVLLLLHVAARVADAVWWRPRRLEAHFAGQGVRGPPYRFLVGCVREMVALMAEA 63

Query: 75  SSHPMPNFS-HNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHF 134
           ++ PMP  + HN LPRVL+FYH+W+KIYG TFLIWFGP  R+ V++P+M+REIF T +  
Sbjct: 64  TAKPMPPAAPHNALPRVLAFYHYWRKIYGPTFLIWFGPTPRLTVAEPEMVREIFLTRAEA 123

Query: 135 YEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLVEESVVEMVEKW 194
           +++ EAHP+V+QLEGDGL++L G KWA HR++++P F+ +NL  L+P V  SV  + E+W
Sbjct: 124 FDRYEAHPVVRQLEGDGLVSLHGDKWAHHRRVLTPGFYPDNLNRLVPHVGRSVAALAERW 183

Query: 195 SSMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQK 254
            +MA     GE+E+DV E FQ + E+ IT+  FG SY+ G+ +F+LQ + M  AS+AF+K
Sbjct: 184 RAMA-CAGGGEVEVDVAEWFQAVAEEAITRATFGRSYDSGRVVFRLQARLMAFASEAFRK 243

Query: 255 VFIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKG--NSEKIWAMDEKKPK------D 314
           V +PGYRFLPT+ N  SW LD++IR+ L++LI  R G    E+     E K K      D
Sbjct: 244 VLVPGYRFLPTKKNRMSWGLDREIRRGLVRLIGRRSGGDGGEEDETTTELKDKQDSGFND 303

Query: 315 LLGLMIQASSLKNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARD 374
           LLGLMI A     + ++ V D+VEECK+FFFAGKQTT+NLLTW TVLLAMHP WQ +AR 
Sbjct: 304 LLGLMINAGV---DRTMPVEDMVEECKTFFFAGKQTTTNLLTWATVLLAMHPDWQDRARR 363

Query: 375 EVFNVCGSRA--LPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELGGR----- 434
           EV  VCG  A  LP+KD +   K L MILNE+LRLYPP VATIRRAK DV LGG      
Sbjct: 364 EVLAVCGDAAGELPTKDHLPKLKTLGMILNETLRLYPPAVATIRRAKFDVTLGGGGDGDA 423

Query: 435 ---MIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRT 494
               IPR TE L+PI+A+HHD  +WG DA++FNP RF+ G  RA  HP  FIPFGLG R 
Sbjct: 424 GGIHIPRDTELLVPIMAIHHDARLWGPDAAQFNPARFASGAARAAKHPLAFIPFGLGSRM 483

Query: 495 CIGQNLAVLEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQ 525
           CIGQ+LA+LEAKLTMA++LQRF   L+PTY HAPTVLMLL+PQYGAP++F+
Sbjct: 484 CIGQSLAILEAKLTMAVLLQRFDLALSPTYVHAPTVLMLLHPQYGAPLIFR 530

BLAST of Lsi06G009380 vs. ExPASy Swiss-Prot
Match: Q8LIF2 (Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 2.0e-148
Identity = 262/515 (50.87%), Postives = 355/515 (68.93%), Query Frame = 0

Query: 15  AAAVTAAVVIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRFFIGNFKELVSMMLAASS 74
           AAA     V   +A+ A  LWW PRR+E+HF  QG+RGP YRFF+G+  ELV +M+ A+S
Sbjct: 20  AAAAAWVAVYAAAARAAEALWWRPRRVERHFAAQGVRGPGYRFFVGSSIELVRLMVDAAS 79

Query: 75  HPM-PNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFYE 134
            PM P  SH+ILPRVL FYHHW+K+YG   LIWFG   R+ VS+P++IRE+  T +  ++
Sbjct: 80  RPMEPPTSHDILPRVLPFYHHWRKLYGPMHLIWFGRTPRLVVSEPELIREVLLTRADHFD 139

Query: 135 KNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLVEESVVEMVEKWSS 194
           + EAHP++ Q EG GL  L G++WA  R++++P FH  NL+++ P V  +V  M+++ + 
Sbjct: 140 RYEAHPMICQFEGYGLSNLHGERWARRRRVLTPAFHTENLRMIAPFVAGTVTRMLDELAE 199

Query: 195 MAESVDSGEIEIDVFECFQTLTEDVITKTAFG-SSYEDGKAIFKLQTQQMILASQAFQKV 254
            A +  +GE E+DV E FQ + ++ IT  AFG  +Y+DG A+F+LQ +    A++A  KV
Sbjct: 200 RARAGGAGEAEVDVAEWFQRVPQEAITFAAFGRRNYDDGAAVFRLQDELAGYATEAHSKV 259

Query: 255 FIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSEKIWAMDEKKPKDLLGLMIQAS 314
           +IPGYRFLPTR N   W+LD++IR  L K +   +  S      D     D  G M +  
Sbjct: 260 YIPGYRFLPTRKNRRVWQLDREIRSHLAKFVTGLQSCSSS-HGDDADDGGDGGGGMREFM 319

Query: 315 SLKNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVFNVCGSR 374
           S     ++T  +I+EE K+FFFAGK+T SNLLTWTTV LAMHP+WQ +AR EV  VCG  
Sbjct: 320 SFM-APAMTAGEIIEESKNFFFAGKETLSNLLTWTTVALAMHPEWQERARREVVAVCGRG 379

Query: 375 ALPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIPILAVH 434
            LP+KD +   K L MILNE+LRLYPP VA IR AK DVELGG ++P GTE +IPI+AVH
Sbjct: 380 DLPTKDHLPKLKTLGMILNETLRLYPPAVAMIRTAKEDVELGGCVVPAGTEVMIPIMAVH 439

Query: 435 HDQSIWGNDASEFNPGRF-SEGVGRAGNHPAGFIPFGLGVRTCIGQNLAVLEAKLTMAII 494
           HD + WG+DA+EFNP RF ++  G    HP  F+PFG G R CIGQN+A++EAK+ +A++
Sbjct: 440 HDAAAWGDDAAEFNPARFAADDDGGRRRHPMAFMPFGGGARVCIGQNMALMEAKVALAVV 499

Query: 495 LQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKI 527
           L+RF FRL+P Y HAP VLM+L PQ+GAPV+F+ +
Sbjct: 500 LRRFEFRLSPAYVHAPRVLMILSPQFGAPVIFRPL 532

BLAST of Lsi06G009380 vs. ExPASy TrEMBL
Match: A0A0A0LU20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G305710 PE=3 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.5e-271
Identity = 468/537 (87.15%), Postives = 498/537 (92.74%), Query Frame = 0

Query: 1   MKIESILMKVPPVWAAAVTAAVVI---ILSAKMALILWWNPRRIEKHFHRQGIRGPPYRF 60
           +KIES      P W+A V   VV+   I++AK+ L+LWW P++IEKHF RQGI+GPPYRF
Sbjct: 4   VKIES------PAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRF 63

Query: 61  FIGNFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 120
           FIGNFKELVS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD
Sbjct: 64  FIGNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 123

Query: 121 PDMIREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLI 180
           PDMIREIF TNSHFYEKNEAHPLVKQLEGDGLLTL GQKWALHRKIISPTFH++NLKLLI
Sbjct: 124 PDMIREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLI 183

Query: 181 PLVEESVVEMVEKWSSMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKL 240
           PLVEESVVEMVEKW SMAES+DSGE+EIDV++ FQTLTEDVITKTAFGSSYEDGKAIFKL
Sbjct: 184 PLVEESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKL 243

Query: 241 QTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSE-KIWAM 300
           QTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLD++I+KSLMKLIE RK N E K W M
Sbjct: 244 QTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGM 303

Query: 301 DEKKPKDLLGLMIQASSLKNNN----SITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLA 360
           +EK+PKDLLGLMIQAS L NNN    SITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLA
Sbjct: 304 NEKRPKDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLA 363

Query: 361 MHPQWQIQARDEVFNVCGSRALPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVE 420
           MHPQWQIQARDEVFNVCG RALPSK DVSNFKILSMILNESLRLYPPTVATIRRAK DVE
Sbjct: 364 MHPQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVE 423

Query: 421 LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVR 480
           L GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV +A NHPAGFIPFGLGVR
Sbjct: 424 LAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVR 483

Query: 481 TCIGQNLAVLEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKIPLN 530
           TCIGQNLA+LEAKLT+AIILQRFTFRLAPTYQHAPTVLMLLYPQYGAP++FQKIP+N
Sbjct: 484 TCIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIPIN 534

BLAST of Lsi06G009380 vs. ExPASy TrEMBL
Match: A0A1S3CMW0 (cytochrome P450 734A1-like OS=Cucumis melo OX=3656 GN=LOC103502730 PE=3 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 9.4e-271
Identity = 472/540 (87.41%), Postives = 497/540 (92.04%), Query Frame = 0

Query: 1   MKIESILMKVPPVW-AAAVTAAV--VIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRF 60
           +KIES      P W AAAVT AV  + I+SAK+ L+LWW P++IEKHF RQGI+GPPYRF
Sbjct: 4   VKIES------PAWSAAAVTTAVLLIFIISAKVGLVLWWKPKKIEKHFDRQGIKGPPYRF 63

Query: 61  FIGNFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 120
           FIGNFKELVS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD
Sbjct: 64  FIGNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 123

Query: 121 PDMIREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLI 180
           PDMIREIF TNS +YEKNEAHPLVKQLEGDGLLTL GQKWALHRKIISPTFH++NLKLLI
Sbjct: 124 PDMIREIFATNSQYYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLI 183

Query: 181 PLVEESVVEMVEKWSSMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKL 240
           PLVEESVVEMVEKW SMAESV+S E+EIDV++ FQTLTEDVITKTAFGSSYEDGKAIFKL
Sbjct: 184 PLVEESVVEMVEKWWSMAESVESSEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKL 243

Query: 241 QTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSE-KIWAM 300
           Q QQMILASQAFQKVFIPGYRFLPTRTNINSWKLDK+I+KSLMKLIE RKGN E K W M
Sbjct: 244 QNQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDKEIKKSLMKLIEGRKGNLEKKNWGM 303

Query: 301 DEKKPKDLLGLMIQASSLKNNN---------SITVHDIVEECKSFFFAGKQTTSNLLTWT 360
           +EK+PKDLLGLMIQAS L NNN         SITVHDIVEECKSFFFAGKQTTSNLLTWT
Sbjct: 304 NEKRPKDLLGLMIQASLLNNNNNNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWT 363

Query: 361 TVLLAMHPQWQIQARDEVFNVCGSRALPSKDDVSNFKILSMILNESLRLYPPTVATIRRA 420
           TVLLAMHPQWQIQARDEV NVCG RALPSK DVSNFKILSMILNESLRLYPPTVATIRRA
Sbjct: 364 TVLLAMHPQWQIQARDEVINVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRA 423

Query: 421 KADVELGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPF 480
           KADVEL GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV RAGNHPAGFIPF
Sbjct: 424 KADVELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSRAGNHPAGFIPF 483

Query: 481 GLGVRTCIGQNLAVLEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKIP 528
           GLGVRTCIGQNLA+LEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAP++FQKIP
Sbjct: 484 GLGVRTCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIP 537

BLAST of Lsi06G009380 vs. ExPASy TrEMBL
Match: A0A6J1H9G1 (cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111461725 PE=3 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 2.5e-255
Identity = 439/526 (83.46%), Postives = 468/526 (88.97%), Query Frame = 0

Query: 4   ESILMKVPPVWAAAVTAAVVIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRFFIGNFK 63
           E +   V   +  A  A VV+IL AKM L+LWW PRRIE HF RQGI GPPYRFFIGNFK
Sbjct: 3   EELESAVEVPFGVAAAAVVVVILGAKMGLVLWWKPRRIENHFLRQGIGGPPYRFFIGNFK 62

Query: 64  ELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRE 123
           ELVSMM AASSHP+PNFSHNILPRVLSFYHHWKKIYGSTF+IWFGPI RVAVSDPDMIRE
Sbjct: 63  ELVSMMAAASSHPIPNFSHNILPRVLSFYHHWKKIYGSTFVIWFGPIARVAVSDPDMIRE 122

Query: 124 IFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLVEES 183
           IF TNSHFYEKNEAHPLVKQLEGDGLLTL G KWALHRKIISPTFH +NLKLLIPLVEES
Sbjct: 123 IFITNSHFYEKNEAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHTDNLKLLIPLVEES 182

Query: 184 VVEMVEKWSSMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMI 243
           V++MV+KWSSM E   SGE+EIDVF+ FQTLTEDVITKTAFG SYEDGKAIFK+QTQQMI
Sbjct: 183 VMDMVQKWSSMTEETTSGEVEIDVFDSFQTLTEDVITKTAFGCSYEDGKAIFKIQTQQMI 242

Query: 244 LASQAFQKVFIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSEKIWAMDEKKPKD 303
           LAS+AFQKVFIPGYRFLPTRTN+ SWKL+K+I+KSL+KLIE RK N EK WA++EK PKD
Sbjct: 243 LASEAFQKVFIPGYRFLPTRTNMKSWKLEKEIKKSLLKLIEGRKRNFEKNWAINEKGPKD 302

Query: 304 LLGLMIQASSLKNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARD 363
           LLGLMIQA       SI+VHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHP+WQI AR 
Sbjct: 303 LLGLMIQA-------SISVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPRWQILARH 362

Query: 364 EVFNVCGSRALPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTE 423
           EVF +CG RALPS DDVS FKILSMILNESLRLYPPTVATIRRA ADVELGGRMIPRGTE
Sbjct: 363 EVFTICGPRALPSSDDVSKFKILSMILNESLRLYPPTVATIRRANADVELGGRMIPRGTE 422

Query: 424 FLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLAVLE 483
           FLIPILAVHHDQ+IWGNDASEFNP RFS+GV RAGNHP GFIPFGLGVR CIGQNLAVLE
Sbjct: 423 FLIPILAVHHDQTIWGNDASEFNPERFSDGVSRAGNHPVGFIPFGLGVRNCIGQNLAVLE 482

Query: 484 AKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKIPLN 530
           AKL M IILQR +FRLAPTYQHAPTVLMLLYPQYGAP+LF+KI LN
Sbjct: 483 AKLAMVIILQRLSFRLAPTYQHAPTVLMLLYPQYGAPILFEKIALN 521

BLAST of Lsi06G009380 vs. ExPASy TrEMBL
Match: A0A6J1L528 (cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111499208 PE=3 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 2.1e-254
Identity = 435/511 (85.13%), Postives = 471/511 (92.17%), Query Frame = 0

Query: 21  AVVIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRFFIGNFKELVSMMLAASSHPMPNF 80
           A++ IL+ KMA+ LWW PRRIE HF RQGIRGPPYRFFIGNFKEL SMMLAA+S PMPNF
Sbjct: 19  ALLFILAVKMAVALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDSMMLAATSRPMPNF 78

Query: 81  SHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFYEKNEAHPL 140
           SHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR+I TTNS FYEKNEAHPL
Sbjct: 79  SHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTTNSEFYEKNEAHPL 138

Query: 141 VKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLVEESVVEMVEKWSSMAESVDS 200
           +K+LEGDGLLTLK QKWALHRKIISP F+M NLKLLIP+VEESVV+MVEKWS+MA    S
Sbjct: 139 IKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDMVEKWSAMAGGAAS 198

Query: 201 GEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQKVFIPGYRFL 260
            E+EIDVF+ FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILAS+AFQKVFIPGYRFL
Sbjct: 199 DEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASEAFQKVFIPGYRFL 258

Query: 261 PTRTNINSWKLDKQIRKSLMKLIESRKGNSEKIWAMDEKKPKDLLGLMIQASSLKNNNS- 320
           PTRTNINSWKLDK+IRKSLMKLIESRKGNSEKIWAMDEK PKDLLGLMIQAS+  +++S 
Sbjct: 259 PTRTNINSWKLDKEIRKSLMKLIESRKGNSEKIWAMDEKAPKDLLGLMIQASNSSSSSSS 318

Query: 321 ----ITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVFNVCGSRALP 380
               I+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEV +VCG RALP
Sbjct: 319 LHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCGPRALP 378

Query: 381 SKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIPILAVHHDQ 440
           S+DD+S FKILSMILNESLRLYPPTVATIRRAK  VEL GRMIPRGTEFLIPILAVHHDQ
Sbjct: 379 SRDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIPRGTEFLIPILAVHHDQ 438

Query: 441 SIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLAVLEAKLTMAIILQRF 500
           +IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+NLA+L+AKL +AII+QRF
Sbjct: 439 NIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKNLALLQAKLAIAIIVQRF 498

Query: 501 TFRLAPTYQHAPTVLMLLYPQYGAPVLFQKI 527
           +FRLAP+YQHAP VLMLLYPQYGAPV+FQKI
Sbjct: 499 SFRLAPSYQHAPAVLMLLYPQYGAPVVFQKI 529

BLAST of Lsi06G009380 vs. ExPASy TrEMBL
Match: A0A6J1JHK6 (cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111485166 PE=3 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 2.1e-254
Identity = 437/517 (84.53%), Postives = 464/517 (89.75%), Query Frame = 0

Query: 13  VWAAAVTAAVVIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRFFIGNFKELVSMMLAA 72
           V AAAV AA V+IL AKM L+LWW PRR+E HF RQGI GP YRFFIGNFKELVSMM AA
Sbjct: 15  VAAAAVVAAAVVILGAKMGLVLWWKPRRMENHFLRQGIGGPRYRFFIGNFKELVSMMAAA 74

Query: 73  SSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFY 132
           SSHPMP FSHNILPRVLSFYHHWKKIYGSTFLIWFGPI RVAVSDPDMIREIF TNSH Y
Sbjct: 75  SSHPMPKFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIARVAVSDPDMIREIFITNSHLY 134

Query: 133 EKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLVEESVVEMVEKWS 192
           EKNEAHPLVKQLEGDGLLTL G KWALHRKIISPTFH +N+KLLIPL+EE +VEMV+KWS
Sbjct: 135 EKNEAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHSDNIKLLIPLIEEIIVEMVQKWS 194

Query: 193 SMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQKV 252
           SMAE    G +EIDVF+ FQTLTEDVITKTAFG SYEDGKAIFKLQTQQMILAS+AFQKV
Sbjct: 195 SMAEETTLGGVEIDVFDSFQTLTEDVITKTAFGCSYEDGKAIFKLQTQQMILASEAFQKV 254

Query: 253 FIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSEKIWAMDEKKPKDLLGLMIQAS 312
           FIPGYRFLPTRTN+ SWKL+K+I+KSL+KLIE RK N EK WA++EK PKDLLGLMIQA 
Sbjct: 255 FIPGYRFLPTRTNMKSWKLEKEIKKSLIKLIEGRKRNFEKNWAINEKGPKDLLGLMIQA- 314

Query: 313 SLKNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVFNVCGSR 372
                 SI+VHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQI AR EVF +CG R
Sbjct: 315 ------SISVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQILARHEVFTICGPR 374

Query: 373 ALPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIPILAVH 432
           ALPS DDVS FKILSMILNESLRLYPPTVATIRRA AD+ELGGRMIPRGTEFLIPILAVH
Sbjct: 375 ALPSPDDVSKFKILSMILNESLRLYPPTVATIRRANADMELGGRMIPRGTEFLIPILAVH 434

Query: 433 HDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLAVLEAKLTMAIIL 492
           HDQ+IWGNDASEFNP RFS+GV RAGNHP GFIPFGLGVR CIGQNLA+LEAKL M IIL
Sbjct: 435 HDQTIWGNDASEFNPERFSDGVSRAGNHPVGFIPFGLGVRNCIGQNLAILEAKLAMVIIL 494

Query: 493 QRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKIPLN 530
           QR +FRLAPTYQHAPTVLMLLYPQYGAP+LF+KIPLN
Sbjct: 495 QRLSFRLAPTYQHAPTVLMLLYPQYGAPILFEKIPLN 524

BLAST of Lsi06G009380 vs. NCBI nr
Match: XP_038880221.1 (cytochrome P450 734A1-like [Benincasa hispida])

HSP 1 Score: 1016.1 bits (2626), Expect = 1.1e-292
Identity = 503/535 (94.02%), Postives = 515/535 (96.26%), Query Frame = 0

Query: 1   MKIESILMKVPPVWAAAVTAAVVIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRFFIG 60
           MKIES+L KVPPVWAAAVTAAVV+ILSAKM L+LWW PRRIEKHF RQGIRGPPYRFFIG
Sbjct: 1   MKIESVLSKVPPVWAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIG 60

Query: 61  NFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM 120
           NFKE+VSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM
Sbjct: 61  NFKEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM 120

Query: 121 IREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLV 180
           IREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHM+NLKLLIPLV
Sbjct: 121 IREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLV 180

Query: 181 EESVVEMVEKWSSMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ 240
           EESVVEMVEKWSSMAES DS E+EIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ
Sbjct: 181 EESVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ 240

Query: 241 QMILASQAFQKVFIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSEKIWAMDEKK 300
           QMILASQAF KVFIPGYRFLPTRTNINSWKLDK+I+KSLMKLIE RK NSEK W MDEK+
Sbjct: 241 QMILASQAFHKVFIPGYRFLPTRTNINSWKLDKEIKKSLMKLIEGRKWNSEKNWGMDEKR 300

Query: 301 PKDLLGLMIQASSLK------NNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMH 360
           PKDLLGLMIQASSLK      NNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMH
Sbjct: 301 PKDLLGLMIQASSLKYNNNNNNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMH 360

Query: 361 PQWQIQARDEVFNVCGSRALPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELG 420
           PQWQIQARDEVFNVCGSRALPSK DVS FKILSMILNESLRLYPPTVATIRRAKADVELG
Sbjct: 361 PQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVELG 420

Query: 421 GRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTC 480
           GRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLG RTC
Sbjct: 421 GRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGARTC 480

Query: 481 IGQNLAVLEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKIPLN 530
           IGQNLA+LEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAP+LFQKIPL+
Sbjct: 481 IGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIPLD 535

BLAST of Lsi06G009380 vs. NCBI nr
Match: XP_011656192.2 (cytochrome P450 734A1 [Cucumis sativus] >KAE8653075.1 hypothetical protein Csa_019999 [Cucumis sativus])

HSP 1 Score: 944.9 bits (2441), Expect = 3.0e-271
Identity = 468/537 (87.15%), Postives = 498/537 (92.74%), Query Frame = 0

Query: 1   MKIESILMKVPPVWAAAVTAAVVI---ILSAKMALILWWNPRRIEKHFHRQGIRGPPYRF 60
           +KIES      P W+A V   VV+   I++AK+ L+LWW P++IEKHF RQGI+GPPYRF
Sbjct: 17  VKIES------PAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRF 76

Query: 61  FIGNFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 120
           FIGNFKELVS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD
Sbjct: 77  FIGNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 136

Query: 121 PDMIREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLI 180
           PDMIREIF TNSHFYEKNEAHPLVKQLEGDGLLTL GQKWALHRKIISPTFH++NLKLLI
Sbjct: 137 PDMIREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLI 196

Query: 181 PLVEESVVEMVEKWSSMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKL 240
           PLVEESVVEMVEKW SMAES+DSGE+EIDV++ FQTLTEDVITKTAFGSSYEDGKAIFKL
Sbjct: 197 PLVEESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKL 256

Query: 241 QTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSE-KIWAM 300
           QTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLD++I+KSLMKLIE RK N E K W M
Sbjct: 257 QTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGM 316

Query: 301 DEKKPKDLLGLMIQASSLKNNN----SITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLA 360
           +EK+PKDLLGLMIQAS L NNN    SITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLA
Sbjct: 317 NEKRPKDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLA 376

Query: 361 MHPQWQIQARDEVFNVCGSRALPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVE 420
           MHPQWQIQARDEVFNVCG RALPSK DVSNFKILSMILNESLRLYPPTVATIRRAK DVE
Sbjct: 377 MHPQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVE 436

Query: 421 LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVR 480
           L GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV +A NHPAGFIPFGLGVR
Sbjct: 437 LAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVR 496

Query: 481 TCIGQNLAVLEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKIPLN 530
           TCIGQNLA+LEAKLT+AIILQRFTFRLAPTYQHAPTVLMLLYPQYGAP++FQKIP+N
Sbjct: 497 TCIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIPIN 547

BLAST of Lsi06G009380 vs. NCBI nr
Match: XP_008465003.1 (PREDICTED: cytochrome P450 734A1-like [Cucumis melo])

HSP 1 Score: 942.2 bits (2434), Expect = 1.9e-270
Identity = 472/540 (87.41%), Postives = 497/540 (92.04%), Query Frame = 0

Query: 1   MKIESILMKVPPVW-AAAVTAAV--VIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRF 60
           +KIES      P W AAAVT AV  + I+SAK+ L+LWW P++IEKHF RQGI+GPPYRF
Sbjct: 4   VKIES------PAWSAAAVTTAVLLIFIISAKVGLVLWWKPKKIEKHFDRQGIKGPPYRF 63

Query: 61  FIGNFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 120
           FIGNFKELVS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD
Sbjct: 64  FIGNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 123

Query: 121 PDMIREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLI 180
           PDMIREIF TNS +YEKNEAHPLVKQLEGDGLLTL GQKWALHRKIISPTFH++NLKLLI
Sbjct: 124 PDMIREIFATNSQYYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLI 183

Query: 181 PLVEESVVEMVEKWSSMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKL 240
           PLVEESVVEMVEKW SMAESV+S E+EIDV++ FQTLTEDVITKTAFGSSYEDGKAIFKL
Sbjct: 184 PLVEESVVEMVEKWWSMAESVESSEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKL 243

Query: 241 QTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSE-KIWAM 300
           Q QQMILASQAFQKVFIPGYRFLPTRTNINSWKLDK+I+KSLMKLIE RKGN E K W M
Sbjct: 244 QNQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDKEIKKSLMKLIEGRKGNLEKKNWGM 303

Query: 301 DEKKPKDLLGLMIQASSLKNNN---------SITVHDIVEECKSFFFAGKQTTSNLLTWT 360
           +EK+PKDLLGLMIQAS L NNN         SITVHDIVEECKSFFFAGKQTTSNLLTWT
Sbjct: 304 NEKRPKDLLGLMIQASLLNNNNNNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWT 363

Query: 361 TVLLAMHPQWQIQARDEVFNVCGSRALPSKDDVSNFKILSMILNESLRLYPPTVATIRRA 420
           TVLLAMHPQWQIQARDEV NVCG RALPSK DVSNFKILSMILNESLRLYPPTVATIRRA
Sbjct: 364 TVLLAMHPQWQIQARDEVINVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRA 423

Query: 421 KADVELGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPF 480
           KADVEL GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV RAGNHPAGFIPF
Sbjct: 424 KADVELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSRAGNHPAGFIPF 483

Query: 481 GLGVRTCIGQNLAVLEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKIP 528
           GLGVRTCIGQNLA+LEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAP++FQKIP
Sbjct: 484 GLGVRTCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIP 537

BLAST of Lsi06G009380 vs. NCBI nr
Match: XP_023516914.1 (cytochrome P450 734A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 893.3 bits (2307), Expect = 1.0e-255
Identity = 439/515 (85.24%), Postives = 465/515 (90.29%), Query Frame = 0

Query: 15  AAAVTAAVVIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRFFIGNFKELVSMMLAASS 74
           A A  AAVV+IL AK+ L+LWW PRRIE HF RQGI GPPYRFFIGNFKELVSMM AASS
Sbjct: 21  AVAAVAAVVVILGAKVGLVLWWKPRRIENHFLRQGIGGPPYRFFIGNFKELVSMMAAASS 80

Query: 75  HPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFYEK 134
           HPMPNFSHNILPRVLSFYHHWKKIYGSTF+IWFGPI RVAVSDPDMIREIF TNSH YEK
Sbjct: 81  HPMPNFSHNILPRVLSFYHHWKKIYGSTFVIWFGPIPRVAVSDPDMIREIFITNSHLYEK 140

Query: 135 NEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLVEESVVEMVEKWSSM 194
           NEAHPLVKQLEGDGLLTL G KWALHRKIISPTFH +NLKLLIPL+EES+VEMV+KWSSM
Sbjct: 141 NEAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHTDNLKLLIPLIEESIVEMVQKWSSM 200

Query: 195 AESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQKVFI 254
           AE   SGE+EIDVF+ FQTLTEDVITKTAFG SY DGKAIFKLQTQQMILAS+AFQKVFI
Sbjct: 201 AEETTSGEVEIDVFDSFQTLTEDVITKTAFGCSYGDGKAIFKLQTQQMILASEAFQKVFI 260

Query: 255 PGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSEKIWAMDEKKPKDLLGLMIQASSL 314
           PGYRFLPTRTN+ SWKL+K+I+KSLMKLIE RK N EK WA++EK PKDLLGLMIQA   
Sbjct: 261 PGYRFLPTRTNMKSWKLEKEIKKSLMKLIEGRKRNFEKNWAINEKGPKDLLGLMIQA--- 320

Query: 315 KNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVFNVCGSRAL 374
               SI+VHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHP+WQI ARDEVF +CG RAL
Sbjct: 321 ----SISVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPRWQILARDEVFTICGPRAL 380

Query: 375 PSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIPILAVHHD 434
           PS DDVS FKILSMILNESLRLYPPTVATIRRA ADV+LGGRMIPRGTEFLI ILAVHHD
Sbjct: 381 PSSDDVSKFKILSMILNESLRLYPPTVATIRRANADVDLGGRMIPRGTEFLISILAVHHD 440

Query: 435 QSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLAVLEAKLTMAIILQR 494
           Q+IWGNDASEFNP RFS+GV RAGNHP GFIPFGLGVR CIGQNLAVLEAKL M IILQR
Sbjct: 441 QTIWGNDASEFNPERFSDGVSRAGNHPVGFIPFGLGVRNCIGQNLAVLEAKLAMVIILQR 500

Query: 495 FTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKIPLN 530
            +FRLAPTYQHAPTVLMLLYPQYGAP+ F+KIPLN
Sbjct: 501 LSFRLAPTYQHAPTVLMLLYPQYGAPIRFEKIPLN 528

BLAST of Lsi06G009380 vs. NCBI nr
Match: XP_022961116.1 (cytochrome P450 734A1-like [Cucurbita moschata])

HSP 1 Score: 891.0 bits (2301), Expect = 5.1e-255
Identity = 439/526 (83.46%), Postives = 468/526 (88.97%), Query Frame = 0

Query: 4   ESILMKVPPVWAAAVTAAVVIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRFFIGNFK 63
           E +   V   +  A  A VV+IL AKM L+LWW PRRIE HF RQGI GPPYRFFIGNFK
Sbjct: 3   EELESAVEVPFGVAAAAVVVVILGAKMGLVLWWKPRRIENHFLRQGIGGPPYRFFIGNFK 62

Query: 64  ELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRE 123
           ELVSMM AASSHP+PNFSHNILPRVLSFYHHWKKIYGSTF+IWFGPI RVAVSDPDMIRE
Sbjct: 63  ELVSMMAAASSHPIPNFSHNILPRVLSFYHHWKKIYGSTFVIWFGPIARVAVSDPDMIRE 122

Query: 124 IFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLVEES 183
           IF TNSHFYEKNEAHPLVKQLEGDGLLTL G KWALHRKIISPTFH +NLKLLIPLVEES
Sbjct: 123 IFITNSHFYEKNEAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHTDNLKLLIPLVEES 182

Query: 184 VVEMVEKWSSMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMI 243
           V++MV+KWSSM E   SGE+EIDVF+ FQTLTEDVITKTAFG SYEDGKAIFK+QTQQMI
Sbjct: 183 VMDMVQKWSSMTEETTSGEVEIDVFDSFQTLTEDVITKTAFGCSYEDGKAIFKIQTQQMI 242

Query: 244 LASQAFQKVFIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSEKIWAMDEKKPKD 303
           LAS+AFQKVFIPGYRFLPTRTN+ SWKL+K+I+KSL+KLIE RK N EK WA++EK PKD
Sbjct: 243 LASEAFQKVFIPGYRFLPTRTNMKSWKLEKEIKKSLLKLIEGRKRNFEKNWAINEKGPKD 302

Query: 304 LLGLMIQASSLKNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARD 363
           LLGLMIQA       SI+VHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHP+WQI AR 
Sbjct: 303 LLGLMIQA-------SISVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPRWQILARH 362

Query: 364 EVFNVCGSRALPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTE 423
           EVF +CG RALPS DDVS FKILSMILNESLRLYPPTVATIRRA ADVELGGRMIPRGTE
Sbjct: 363 EVFTICGPRALPSSDDVSKFKILSMILNESLRLYPPTVATIRRANADVELGGRMIPRGTE 422

Query: 424 FLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLAVLE 483
           FLIPILAVHHDQ+IWGNDASEFNP RFS+GV RAGNHP GFIPFGLGVR CIGQNLAVLE
Sbjct: 423 FLIPILAVHHDQTIWGNDASEFNPERFSDGVSRAGNHPVGFIPFGLGVRNCIGQNLAVLE 482

Query: 484 AKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKIPLN 530
           AKL M IILQR +FRLAPTYQHAPTVLMLLYPQYGAP+LF+KI LN
Sbjct: 483 AKLAMVIILQRLSFRLAPTYQHAPTVLMLLYPQYGAPILFEKIALN 521

BLAST of Lsi06G009380 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 753.4 bits (1944), Expect = 1.2e-217
Identity = 370/528 (70.08%), Postives = 440/528 (83.33%), Query Frame = 0

Query: 1   MKIESILMKVPPVWAAAVTAAVVIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRFFIG 60
           M+ ES    +P V   +V  ++VI+   K   +LWW PR+IE+HF +QGIRGPPY FFIG
Sbjct: 1   MEEESSSWFIPKVLVLSVILSLVIV---KGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIG 60

Query: 61  NFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM 120
           N KELV MML ASSHPMP FSHNILPRVLSFYHHW+KIYG+TFL+WFGP  R+ V+DPD+
Sbjct: 61  NVKELVGMMLKASSHPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDL 120

Query: 121 IREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLV 180
           IREIF + S FYEKNEAHPLVKQLEGDGLL+LKG+KWA HRKIISPTFHM NLKLL+P+V
Sbjct: 121 IREIF-SKSEFYEKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVV 180

Query: 181 EESVVEMVEKWSSMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ 240
            +SV +MV+KWS      ++GE+E+DV+E FQ LTEDVI++TAFGSSYEDG+A+F+LQ Q
Sbjct: 181 LKSVTDMVDKWSDKLS--ENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQ 240

Query: 241 QMILASQAFQKVFIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSEKIWAMDEKK 300
           QM+L ++AFQKVFIPGYRF PTR N+ SWKLDK+IRKSL+KLIE R+ N+      + K+
Sbjct: 241 QMLLCAEAFQKVFIPGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKE 300

Query: 301 P--KDLLGLMIQASSLKNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQ 360
           P  KDLLGLMIQA       ++TV DIVEECKSFFFAGKQTTSNLLTWTT+LL+MHP+WQ
Sbjct: 301 PAAKDLLGLMIQA------KNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQ 360

Query: 361 IQARDEVFNVCGSRALPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELGGRMI 420
            +ARDEV  VCGSR +P+KD V   K LSMILNESLRLYPP VATIRRAK+DV+LGG  I
Sbjct: 361 AKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKI 420

Query: 421 PRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQN 480
           P GTE LIPI+AVHHDQ+IWGND +EFNP RF++GV RA  HP GFIPFGLGVRTCIGQN
Sbjct: 421 PCGTELLIPIIAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQN 480

Query: 481 LAVLEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKI 527
           LA+L+AKLT+A+++QRFTF LAPTYQHAPTVLMLLYPQ+GAP+ F+++
Sbjct: 481 LAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRL 515

BLAST of Lsi06G009380 vs. TAIR 10
Match: AT3G14620.1 (cytochrome P450, family 72, subfamily A, polypeptide 8 )

HSP 1 Score: 408.3 bits (1048), Expect = 9.4e-114
Identity = 206/518 (39.77%), Postives = 321/518 (61.97%), Query Frame = 0

Query: 15  AAAVTAAVVIILSAKMAL-----ILWWNPRRIEKHFHRQGIRGPPYRFFIGNFKELVSMM 74
           A AV AAVV++ +  + +     + W  P++ E +  RQG+ G P+ F +G+ K   SM+
Sbjct: 8   AVAVAAAVVVVTTVTVWIWKGLNVAWLRPKKNEAYLKRQGLSGTPFTFLVGDIKREASMV 67

Query: 75  LAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNS 134
               S P+ N + +   RV+       K +G T  +W GPI  V V+ P+ I+++     
Sbjct: 68  EQEKSRPI-NLTDDYTHRVMPLIQQTVKDHGKTSYMWMGPIASVIVTKPEHIKDVL-NRV 127

Query: 135 HFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLVEESVVEMVE 194
           + + K   HP+V +L   G+   +G+KW+ HRKII+P+FH+  LK++IP   ES  EM+ 
Sbjct: 128 YDFPKPPVHPIV-ELFATGVALYEGEKWSKHRKIINPSFHLEKLKIMIPAFYESCSEMIS 187

Query: 195 KWSSMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAF 254
           KW  +     S   EIDV+     LT DVI++TAFGSSYE+GK IF+LQ +Q     +A 
Sbjct: 188 KWEKLVTEQGSSN-EIDVWPYLGDLTSDVISRTAFGSSYEEGKRIFELQEEQGRRVLKAL 247

Query: 255 QKVFIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSEKIWAMDEKKPKDLLGLMI 314
           +  FIPG RFLPT+ N+   +++K+++  L ++I  R+   +      E    DLLG+++
Sbjct: 248 ELAFIPGMRFLPTKNNLRMRQINKEVKSRLREIIMKRQRGMD----TGEAPKNDLLGILL 307

Query: 315 QASSLKNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVFNVC 374
           +++S   ++ +++ D+VEEC+ F FAG++TT+ LL WT ++L+ H +WQ QAR+E+  V 
Sbjct: 308 ESNS--GDHGMSIEDVVEECRLFHFAGQETTAVLLVWTMIMLSHHQKWQDQAREEILKVI 367

Query: 375 GSRALPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELGGRM-IPRGTEFLIPI 434
           G    P+ D +S  K +SMILNE LRLYPP +   R  + + +LG  M +P G + +IP+
Sbjct: 368 GKNNKPNFDALSRLKTMSMILNEVLRLYPPGILLGRTVEKETKLGEDMTLPGGAQVVIPV 427

Query: 435 LAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLAVLEAKLTM 494
           L VH D  +WG D  EFNP RF++G+ +A  +   F+PFG G R C GQN A++EAK+ +
Sbjct: 428 LMVHRDPELWGEDVHEFNPERFADGISKATKNQVSFLPFGWGPRFCPGQNFALMEAKMAL 487

Query: 495 AIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKI 527
            +ILQRF+F L+P+Y HAP  ++ L+PQ+GAP++F  +
Sbjct: 488 VLILQRFSFELSPSYTHAPHTVLTLHPQFGAPLIFHML 515

BLAST of Lsi06G009380 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 406.8 bits (1044), Expect = 2.7e-113
Identity = 197/510 (38.63%), Postives = 310/510 (60.78%), Query Frame = 0

Query: 21  AVVIILSAKM---ALILWWNPRRIEKHFHRQGIRGPPYRFFIGNFKELVSMMLAASSHPM 80
           A+V+++  K+     IL W P  + + F +QGI GP YR   GN +E+  M   A    +
Sbjct: 68  ALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREIRKMKNEAKLMVL 127

Query: 81  PNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFYEKNEA 140
              S++I+PRVL     WK  YG TFL W G   R+ +SD ++ ++I +    F+ K++ 
Sbjct: 128 DPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSKSKT 187

Query: 141 HPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLVEESVVEMVEKWSSMAES 200
            P + +L G+GL+ + G  W  HR+I++P F M+ LKL+  L+ +    M  +W      
Sbjct: 188 KPEILKLSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKKQRNG 247

Query: 201 VDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQKVFIPGY 260
           V++ +  + +   F+ LT D+I   AFGSSY +G  +FK Q +     + A   ++ PG 
Sbjct: 248 VETEQFVL-ISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGI 307

Query: 261 RFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSEKIWAMDEKKPKDLLGLMI-QASSLKN 320
           ++LPT +N+  WKLD ++  S+ ++I++R  +  K +        DLLG+M+  ASS ++
Sbjct: 308 QYLPTPSNLQIWKLDMKVNSSIKRIIDARLTSESKDYG------NDLLGIMLTAASSNES 367

Query: 321 NNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVFNVCGSRALPS 380
              +++ +I+EECK+FFFAG +TT+NLLTW+T+LL++H  WQ + R+EVFN CG   +P 
Sbjct: 368 EKKMSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPD 427

Query: 381 KDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIPILAVHHDQS 440
            +  S  K+++ +  ESLRLY P +  +R A  D++LG   IP+GT  ++PI  +H D++
Sbjct: 428 AETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKA 487

Query: 441 IWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLAVLEAKLTMAIILQRFT 500
           +WG+DA +FNP RF+ G+ RA NHP   + F +G R CIGQN A++EAK  +A+ILQRF 
Sbjct: 488 VWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFR 547

Query: 501 FRLAPTYQHAPTVLMLLYPQYGAPVLFQKI 527
             L+  Y+HAP   + L PQY  PV+ + I
Sbjct: 548 LNLSADYKHAPADHLTLQPQYDLPVILEPI 570

BLAST of Lsi06G009380 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 401.4 bits (1030), Expect = 1.2e-111
Identity = 206/520 (39.62%), Postives = 317/520 (60.96%), Query Frame = 0

Query: 8   MKVPPVWAAAVTAAVVIILSAKMALILWWNPRRIEKHFHRQGIRGPPYRFFIGNFKELVS 67
           M    V A  V  AVV++ + ++   +W  P+ +E    RQG+ G PY   +G+ K  V 
Sbjct: 1   MSFSVVAALPVLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVD 60

Query: 68  MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTT 127
           MM+ A S P+ N + +I PR+L         +G TF IW GP+  + +++P+ I+E+F  
Sbjct: 61  MMMEARSKPI-NVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNK 120

Query: 128 NSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMNNLKLLIPLVEESVVEM 187
            + F EK    PL++ L G GL + KG KWA HR+II+P FH+  +K +IP       E+
Sbjct: 121 VNDF-EKASTFPLIRLLAG-GLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEV 180

Query: 188 VEKWSSMAESVDSGEIEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQ 247
           V +W  +    +S  +E+DV+     +T DVI+ TAFGSSY++G+ IF+LQ +   L +Q
Sbjct: 181 VCQWEKLFTDKES-PLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQ 240

Query: 248 AFQKVFIPGYRFLPTRTNINSWKLDKQIRKSLMKLIESRKGNSEKIWAMDEKKPKDLLGL 307
           AF+K +IPG RF PT++N     +D+++   L  ++  R    EK     E    DLLG+
Sbjct: 241 AFKKSYIPGSRFYPTKSNRRMKAIDREVDVILRGIVSKR----EKAREAGEPANDDLLGI 300

Query: 308 MIQASSLKN-NNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVF 367
           +++++S ++  N ++V D+++ECK F+FAG++TTS LL WT VLL+ H  WQ +AR+EV 
Sbjct: 301 LLESNSEESQGNGMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVM 360

Query: 368 NVCGSRALPSKDDVSNFKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLI 427
            V G    P  + ++N K+++MI NE LRLYPP     R    +++LG   +P G +  +
Sbjct: 361 QVLGENNKPDMESLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYL 420

Query: 428 PILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLAVLEAKL 487
           P + V  D  +WG+DA++F P RF +G+ +A  +   F PFG G R CIGQN A+LEAK+
Sbjct: 421 PTILVQRDTELWGDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKM 480

Query: 488 TMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPVLFQKI 527
            MA+ILQ+F+F L+P+Y HAP  +M   PQ+GA ++  K+
Sbjct: 481 AMALILQKFSFELSPSYVHAPQTVMTTRPQFGAHLILHKL 512

BLAST of Lsi06G009380 vs. TAIR 10
Match: AT1G75130.1 (cytochrome P450, family 721, subfamily A, polypeptide 1 )

HSP 1 Score: 400.6 bits (1028), Expect = 2.0e-111
Identity = 213/499 (42.69%), Postives = 302/499 (60.52%), Query Frame = 0

Query: 36  WNPRRIEKHFHRQGIRGPPYRFFIGNFKELVSMMLAASSHPMPN--FSHNILPRVLSFYH 95
           W P RI+ HF +Q + GP YR F GN  E+  +   A S P+P+    H  + RV   YH
Sbjct: 24  WVPWRIQSHFKKQSVTGPSYRIFSGNSGEVSRLTAEAKSKPIPSGRNPHEFVHRVAPHYH 83

Query: 96  HWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLK 155
            W ++YG TFL WFG    VA SDP +IRE  TT   F ++   +PL K L   GL  L+
Sbjct: 84  EWSRVYGKTFLYWFGSKPVVATSDPRLIREALTTGGSF-DRIGHNPLSKLLYAQGLPGLR 143

Query: 156 GQKWALHRKIISPTFHMNNLKLLIPLVEESVVEMVEKWSSMAESVDSGEIEIDVFECFQT 215
           G +WA HR+I    F M  LK  +P +  S + ++EKW  M    +  EIE++V +    
Sbjct: 144 GDQWAFHRRIAKQAFTMEKLKRWVPQMVTSTMMLMEKWEDMRNGGE--EIELEVHKEMHN 203

Query: 216 LTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDK 275
           L+ +++++TAFG+S E+GK IF+LQ + M L       V+IPG+RF P++TN   W+++K
Sbjct: 204 LSAEMLSRTAFGNSVEEGKGIFELQERMMRLFYLVRWSVYIPGFRFFPSKTNREIWRIEK 263

Query: 276 QIRKSLMKLIESRKGNSEKIWAMDEKKPKDLLGLMIQA--SSLKNNN----SITVHDIVE 335
           QIR S++KLIE+ K   EK             G ++QA  S   N N     + + ++ +
Sbjct: 264 QIRVSILKLIENNKTAVEK------------SGTLLQAFMSPYTNQNGQEEKLGIEEVTD 323

Query: 336 ECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVFNVCGSRALPSKDDVSNFKILS 395
           ECK+F+FA K+TT+NL+T+  VLLAM+ +WQ  AR+EV  V G   LP+ D + + K LS
Sbjct: 324 ECKTFYFAAKETTANLMTFVLVLLAMNQEWQNIAREEVICVLGQTGLPTLDILQDLKTLS 383

Query: 396 MILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNP 455
           MI+NE+LRLYPP +   R      +LG   IP GT+  + ++A+HHD+  WG+DA EFNP
Sbjct: 384 MIINETLRLYPPAMTLNRDTLKRAKLGDLDIPAGTQLYLSVVAMHHDKETWGDDAEEFNP 443

Query: 456 GRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLAVLEAKLTMAIILQRFTFRLAPTYQHAP 515
            RF +         A  +PFGLG RTC+GQNLAV EAK  +A IL+ ++FRL+P+Y HAP
Sbjct: 444 RRFED----PKKQSALLVPFGLGPRTCVGQNLAVNEAKTVLATILKYYSFRLSPSYAHAP 503

Query: 516 TVLMLLYPQYGAPVLFQKI 527
            + + L PQ GA +LF +I
Sbjct: 504 VLFVTLQPQNGAHLLFTRI 503

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O487861.7e-21670.08Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1[more]
B9X2874.6e-19061.26Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 ... [more]
Q6Z6D61.5e-17757.60Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 ... [more]
Q69XM69.0e-17055.93Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 ... [more]
Q8LIF22.0e-14850.87Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LU201.5e-27187.15Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G305710 PE=3 SV=1[more]
A0A1S3CMW09.4e-27187.41cytochrome P450 734A1-like OS=Cucumis melo OX=3656 GN=LOC103502730 PE=3 SV=1[more]
A0A6J1H9G12.5e-25583.46cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111461725 PE=3 SV... [more]
A0A6J1L5282.1e-25485.13cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111499208 PE=3 SV=1[more]
A0A6J1JHK62.1e-25484.53cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111485166 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038880221.11.1e-29294.02cytochrome P450 734A1-like [Benincasa hispida][more]
XP_011656192.23.0e-27187.15cytochrome P450 734A1 [Cucumis sativus] >KAE8653075.1 hypothetical protein Csa_0... [more]
XP_008465003.11.9e-27087.41PREDICTED: cytochrome P450 734A1-like [Cucumis melo][more]
XP_023516914.11.0e-25585.24cytochrome P450 734A1-like [Cucurbita pepo subsp. pepo][more]
XP_022961116.15.1e-25583.46cytochrome P450 734A1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G26710.11.2e-21770.08Cytochrome P450 superfamily protein [more]
AT3G14620.19.4e-11439.77cytochrome P450, family 72, subfamily A, polypeptide 8 [more]
AT2G46950.12.7e-11338.63cytochrome P450, family 709, subfamily B, polypeptide 2 [more]
AT3G14610.11.2e-11139.62cytochrome P450, family 72, subfamily A, polypeptide 7 [more]
AT1G75130.12.0e-11142.69cytochrome P450, family 721, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 474..485
score: 43.42
coord: 335..352
score: 38.91
coord: 465..474
score: 56.5
coord: 388..399
score: 39.28
IPR001128Cytochrome P450PFAMPF00067p450coord: 89..507
e-value: 2.5E-86
score: 290.3
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 214..232
score: 21.49
coord: 344..370
score: 28.07
coord: 464..474
score: 54.13
coord: 474..497
score: 38.67
coord: 96..115
score: 29.15
coord: 324..341
score: 30.53
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 34..525
e-value: 7.3E-117
score: 392.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 49..524
NoneNo IPR availablePANTHERPTHR24282:SF63CYTOCHROMEA2coord: 13..521
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 13..521
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 467..476

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G009380.1Lsi06G009380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016131 brassinosteroid metabolic process
biological_process GO:0009741 response to brassinosteroid
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0008395 steroid hydroxylase activity
molecular_function GO:0004497 monooxygenase activity