Lsi06G009140 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G009140
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Protein argonaute PNH1-like
Location	chr06: 18867150 .. 18868494 (+)
RNA-Seq Expression	Lsi06G009140
Synteny	Lsi06G009140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGTGATCATATCAGGGAACGAGAATTTAAAGTGCAGATAAAGTTTGTAACGTTGGCTAGCATGCACCAGTTGCGAGAATTACTCACTGGCAAACAAGTAAATAATCCTCAAGAAGCTTTGACCATTATTGACATCGTCTTGAGGGAGCTCCATGCTCAAAGGTTTGTATCTAATGGCTCTTTTTTGGCCCTTGTTTGTTGGATTCATGTTTTGGCTGCATTTAATTCTGTGGTGAATTTCAATCTGGAATTATAGATATATATCAGTTGGGCGATCCTTTTACTCTCCTTATATTAAGAAGCCTCAGAATGTTGGGGGTGGTTTGCAAGCATGGCGTGGCTTCTATCAAAGCATCCGACCTACTCAAATGGGATTGTCACTAAATATTGGTGGGCATACGAACTTCGTTCCATTTAGAGTTTTCTTTTCCTATGCTTCTAATGTTTTTTCAGACTGAATTGACTTCTCTTATGGTTTTCTTTTTGAAATTGCATCTCATATCAAGACATGTCGTCCACTGCATTCATTGAAGCAATCCCTGTCATTGACTTTGTCGCTCAACTTTTAAACAAAGATGTGTACTCGAGAACATTGTCTGATGCTGATCGTATTAAGGTACCATGAGATTTATGGACACTTTTAGCTTCTTGAGAATGAAATTTAGGTTATAATATCAGAGTTTGAAAGATCCTGCACTGAACAAGTATGGATGCCTTTTTGCAAGGTCAAAAAAGTACTTAGAGGTGTAAAAGTTGAAGTTACACACAGAGGAAATGTACGAAGGAAATATCGAATTTCTGGGCTAACATCACAGCCTACAAGAGAGCTAATGTATATAAAATCTTGGCCCCATCCTTATTCTCCATTTCAGATTGTCTTTTTCTCTTTGATGTTGTTTCAATTGAGTTGTATTGCTGCAGTTTCCCTCTTGATGAGCAGATGAACATGAAATCTGTAGTTGAGTACTTCCAAGAGATGTATGGATATACCATTCAGTATACACATTTGCCTTGCCTTCAAGTAGGAAACCAGAAGAAGGTGAATTATCTACCCATGGAGGTCAGTCCCGCCAACTATACCCCTCGATGCCTTCTACTACATTTGAATCTTTTGTCTGCAGAAAATTTACGTGACATTTTTCCCCAGGCATGTAAGATACTCAAAGGACAGAGATATACAAAAGGACTGAATGAAAAGCAAATAACTTCCTTGTTGAAGGTTTCGTGCCAAAGACCCTCTGATCAGGAAATGGACATTTTACAGGTCCAATACATAATTTTTTTTCCTCTCAATTTGCTAATTACCATTGTCTTGGACCATGCAAAGACATTAACTGTGTAA

mRNA sequence

ATGGGGGAACGAGAATTTAAAGTGCAGATAAAGTTTGTAACGTTGGCTAGCATGCACCAGTTGCGAGAATTACTCACTGGCAAACAAGTAAATAATCCTCAAGAAGCTTTGACCATTATTGACATCGTCTTGAGGGAGCTCCATGCTCAAAGATATATATCAGTTGGGCGATCCTTTTACTCTCCTTATATTAAGAAGCCTCAGAATGTTGGGGGTGGTTTGCAAGCATGGCGTGGCTTCTATCAAAGCATCCGACCTACTCAAATGGGATTGTCACTAAATATTGACATGTCGTCCACTGCATTCATTGAAGCAATCCCTGTCATTGACTTTGTCGCTCAACTTTTAAACAAAGATGTGTACTCGAGAACATTGTCTGATGCTGATCGTATTAAGGTTATAATATCAGAGTTTGAAAGATCCTGCACTGAACAAGTATGGATGCCTTTTTGCAAGGTCAAAAAAGTACTTAGAGGTGTAAAAGTTGAAGTTACACACAGAGGAAATGTACGAAGGAAATATCGAATTTCTGGGCTAACATCACAGCCTACAAGAGAGCTAATTTTCCCTCTTGATGAGCAGATGAACATGAAATCTGTAGTTGAGTACTTCCAAGAGATGTATGGATATACCATTCAGTATACACATTTGCCTTGCCTTCAAGTAGGAAACCAGAAGAAGGTGAATTATCTACCCATGGAGGTCAGTCCCGCCAACTATACCCCTCGATGCCTTCTACTACATTTGAATCTTTTGTCTGCAGAAAATTTACGTGACATTTTTCCCCAGGCATGTAAGATACTCAAAGGACAGAGATATACAAAAGGACTGAATGAAAAGCAAATAACTTCCTTGTTGAAGGTTTCGTGCCAAAGACCCTCTGATCAGGAAATGGACATTTTACAGGTCCAATACATAATTTTTTTTCCTCTCAATTTGCTAATTACCATTGTCTTGGACCATGCAAAGACATTAACTGTGTAA

Coding sequence (CDS)

ATGGGGGAACGAGAATTTAAAGTGCAGATAAAGTTTGTAACGTTGGCTAGCATGCACCAGTTGCGAGAATTACTCACTGGCAAACAAGTAAATAATCCTCAAGAAGCTTTGACCATTATTGACATCGTCTTGAGGGAGCTCCATGCTCAAAGATATATATCAGTTGGGCGATCCTTTTACTCTCCTTATATTAAGAAGCCTCAGAATGTTGGGGGTGGTTTGCAAGCATGGCGTGGCTTCTATCAAAGCATCCGACCTACTCAAATGGGATTGTCACTAAATATTGACATGTCGTCCACTGCATTCATTGAAGCAATCCCTGTCATTGACTTTGTCGCTCAACTTTTAAACAAAGATGTGTACTCGAGAACATTGTCTGATGCTGATCGTATTAAGGTTATAATATCAGAGTTTGAAAGATCCTGCACTGAACAAGTATGGATGCCTTTTTGCAAGGTCAAAAAAGTACTTAGAGGTGTAAAAGTTGAAGTTACACACAGAGGAAATGTACGAAGGAAATATCGAATTTCTGGGCTAACATCACAGCCTACAAGAGAGCTAATTTTCCCTCTTGATGAGCAGATGAACATGAAATCTGTAGTTGAGTACTTCCAAGAGATGTATGGATATACCATTCAGTATACACATTTGCCTTGCCTTCAAGTAGGAAACCAGAAGAAGGTGAATTATCTACCCATGGAGGTCAGTCCCGCCAACTATACCCCTCGATGCCTTCTACTACATTTGAATCTTTTGTCTGCAGAAAATTTACGTGACATTTTTCCCCAGGCATGTAAGATACTCAAAGGACAGAGATATACAAAAGGACTGAATGAAAAGCAAATAACTTCCTTGTTGAAGGTTTCGTGCCAAAGACCCTCTGATCAGGAAATGGACATTTTACAGGTCCAATACATAATTTTTTTTCCTCTCAATTTGCTAATTACCATTGTCTTGGACCATGCAAAGACATTAACTGTGTAA

Protein sequence

MGEREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSPYIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYSRTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQPTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTPRCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQVQYIIFFPLNLLITIVLDHAKTLTV

Homology

[Close]

BLAST of Lsi06G009140 vs. ExPASy Swiss-Prot
Match: Q69VD5 (Protein argonaute PNH1 OS=Oryza sativa subsp. japonica OX=39947 GN=PHN1 PE=2 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 2.9e-102
Identity = 193/300 (64.33%), Postives = 224/300 (74.67%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           ERE++V IKF   A +H LR+ + G+Q + PQEAL ++DIVLREL  +RY+S+GRSFYSP
Sbjct: 229 EREYRVAIKFAARADLHHLRQFIAGRQADAPQEALQVLDIVLRELANRRYVSIGRSFYSP 288

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            I+KPQ +G GLQ+W GFYQSIRPTQMGLSLNIDMSSTAFIE +PVI+FVAQ+L KDV S
Sbjct: 289 DIRKPQRLGDGLQSWCGFYQSIRPTQMGLSLNIDMSSTAFIEPLPVIEFVAQILGKDVIS 348

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           R LSDA+RI                    K+KK LRGVKVEVTHRGNVRRKYRISGLT+Q
Sbjct: 349 RPLSDANRI--------------------KIKKALRGVKVEVTHRGNVRRKYRISGLTTQ 408

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
           PT ELIFP+D+QMNMKSVVEYF+EMYG+TIQ+ HLPCLQVGNQKK NYLPME        
Sbjct: 409 PTHELIFPIDDQMNMKSVVEYFKEMYGFTIQHPHLPCLQVGNQKKANYLPME-------- 468

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                ACKI++GQRYTK LNEKQITSLLKV+C+RP +QEMDILQ
Sbjct: 469 ---------------------ACKIVEGQRYTKRLNEKQITSLLKVTCRRPREQEMDILQ 479

[Close]

BLAST of Lsi06G009140 vs. ExPASy Swiss-Prot
Match: Q9XGW1 (Protein argonaute 10 OS=Arabidopsis thaliana OX=3702 GN=AGO10 PE=1 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 2.7e-95
Identity = 183/300 (61.00%), Postives = 217/300 (72.33%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           ER +KV IKFV  A+MH L E L GK+ + PQEA+ I+DIVLREL  +R+  VGRSF+SP
Sbjct: 234 ERSYKVAIKFVARANMHHLGEFLAGKRADCPQEAVQILDIVLRELSVKRFCPVGRSFFSP 293

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            IK PQ +G GL++W GFYQSIRPTQMGLSLNIDM+S AFIE +PVI+FVAQLL KDV S
Sbjct: 294 DIKTPQRLGEGLESWCGFYQSIRPTQMGLSLNIDMASAAFIEPLPVIEFVAQLLGKDVLS 353

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           + LSD+DR+                    K+KK LRGVKVEVTHR NVRRKYR++GLT+Q
Sbjct: 354 KPLSDSDRV--------------------KIKKGLRGVKVEVTHRANVRRKYRVAGLTTQ 413

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
           PTREL+FP+DE   MKSV+EYFQEMYG+TIQ+THLPCLQVGNQKK +YLPME        
Sbjct: 414 PTRELMFPVDENCTMKSVIEYFQEMYGFTIQHTHLPCLQVGNQKKASYLPME-------- 473

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                ACKI++GQRYTK LNEKQIT+LLKV+CQRP D+E DIL+
Sbjct: 474 ---------------------ACKIVEGQRYTKRLNEKQITALLKVTCQRPRDRENDILR 484

[Close]

BLAST of Lsi06G009140 vs. ExPASy Swiss-Prot
Match: O04379 (Protein argonaute 1 OS=Arabidopsis thaliana OX=3702 GN=AGO1 PE=1 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 8.8e-91
Identity = 181/300 (60.33%), Postives = 207/300 (69.00%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           EREFKV IK V  A +H L   L GKQ + PQEAL ++DIVLREL   RYI VGRSFYSP
Sbjct: 284 EREFKVVIKLVARADLHHLGMFLEGKQSDAPQEALQVLDIVLRELPTSRYIPVGRSFYSP 343

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            I K Q++G GL++WRGFYQSIRPTQMGLSLNIDMSSTAFIEA PVI FV  LLN+D+ S
Sbjct: 344 DIGKKQSLGDGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEANPVIQFVCDLLNRDISS 403

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           R LSDADR+                    K+KK LRGVKVEVTHRGN+RRKYRISGLT+ 
Sbjct: 404 RPLSDADRV--------------------KIKKALRGVKVEVTHRGNMRRKYRISGLTAV 463

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
            TREL FP+DE+   KSVVEYF E YG+ IQ+T LPCLQVGN  + NYLPMEV       
Sbjct: 464 ATRELTFPVDERNTQKSVVEYFHETYGFRIQHTQLPCLQVGNSNRPNYLPMEV------- 523

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                 CKI++GQRY+K LNE+QIT+LLKV+CQRP D+E DILQ
Sbjct: 524 ----------------------CKIVEGQRYSKRLNERQITALLKVTCQRPIDREKDILQ 534

[Close]

BLAST of Lsi06G009140 vs. ExPASy Swiss-Prot
Match: Q6EU14 (Protein argonaute 1A OS=Oryza sativa subsp. japonica OX=39947 GN=AGO1A PE=2 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 4.4e-90
Identity = 176/300 (58.67%), Postives = 209/300 (69.67%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           ER FKV IKF   A +H L   L G+Q + PQEAL ++DIVLREL   RY  V RSFYSP
Sbjct: 316 ERPFKVVIKFAARADLHHLAMFLAGRQADAPQEALQVLDIVLRELPTARYSPVARSFYSP 375

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            + + Q +G GL++WRGFYQSIRPTQMGLSLNIDMSSTAFIE +PVIDFVAQLLN+D+  
Sbjct: 376 NLGRRQQLGEGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEPLPVIDFVAQLLNRDISV 435

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           R LSDADR+                    K+KK LRGVKVEVTHRGN+RRKYRISGLTSQ
Sbjct: 436 RPLSDADRV--------------------KIKKALRGVKVEVTHRGNMRRKYRISGLTSQ 495

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
            TREL FP+D    +K+VV+YFQE YG+ I++T LPCLQVGNQ++ NYLPMEV       
Sbjct: 496 ATRELSFPIDNHGTVKTVVQYFQETYGFNIKHTTLPCLQVGNQQRPNYLPMEV------- 555

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                 CKI++GQRY+K LNEKQIT+LLKV+CQRP ++E+DILQ
Sbjct: 556 ----------------------CKIVEGQRYSKRLNEKQITALLKVTCQRPQERELDILQ 566

[Close]

BLAST of Lsi06G009140 vs. ExPASy Swiss-Prot
Match: Q7XSA2 (Protein argonaute 1B OS=Oryza sativa subsp. japonica OX=39947 GN=AGO1B PE=2 SV=3)

HSP 1 Score: 330.5 bits (846), Expect = 2.2e-89
Identity = 174/300 (58.00%), Postives = 212/300 (70.67%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           ER F+V IKF   A +H L   L G+Q + PQEAL ++DIVLREL   RY  VGRSFYSP
Sbjct: 353 ERLFRVVIKFAARADLHHLAMFLAGRQADAPQEALQVLDIVLRELPTTRYSPVGRSFYSP 412

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            + + Q +G GL++WRGFYQSIRPTQMGLSLNIDMSSTAFIE +PVIDFVAQLLN+D+  
Sbjct: 413 NLGRRQQLGEGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEPLPVIDFVAQLLNRDISV 472

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           R LSD+DR+                    K+KK LRGVKVEVTHRGN+RRKYRISGLTSQ
Sbjct: 473 RPLSDSDRV--------------------KIKKALRGVKVEVTHRGNMRRKYRISGLTSQ 532

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
            TREL FP+D++  +K+VV+YF E YG++IQ+T LPCLQVGNQ++ NYLPMEV       
Sbjct: 533 ATRELSFPVDDRGTVKTVVQYFLETYGFSIQHTTLPCLQVGNQQRPNYLPMEV------- 592

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                 CKI++GQRY+K LNEKQIT+LLKV+CQRP ++E+DIL+
Sbjct: 593 ----------------------CKIVEGQRYSKRLNEKQITALLKVTCQRPQERELDILR 603

[Close]

BLAST of Lsi06G009140 vs. ExPASy TrEMBL
Match: A0A1S3CJ14 (protein argonaute PNH1-like OS=Cucumis melo OX=3656 GN=LOC103501520 PE=3 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 6.1e-127
Identity = 248/300 (82.67%), Postives = 249/300 (83.00%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           EREFKVQIKFVTLASMHQLRELL GKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP
Sbjct: 177 EREFKVQIKFVTLASMHQLRELLAGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 236

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            IKKPQ+VGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS
Sbjct: 237 CIKKPQHVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 296

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           RTLSDADRI                    KVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ
Sbjct: 297 RTLSDADRI--------------------KVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 356

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
           PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPME        
Sbjct: 357 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPME-------- 416

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                ACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ
Sbjct: 417 ---------------------ACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 427

[Close]

BLAST of Lsi06G009140 vs. ExPASy TrEMBL
Match: A0A5D3BIT5 (Protein argonaute PNH1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold222G00080 PE=3 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 6.1e-127
Identity = 248/300 (82.67%), Postives = 249/300 (83.00%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           EREFKVQIKFVTLASMHQLRELL GKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP
Sbjct: 177 EREFKVQIKFVTLASMHQLRELLAGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 236

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            IKKPQ+VGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS
Sbjct: 237 CIKKPQHVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 296

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           RTLSDADRI                    KVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ
Sbjct: 297 RTLSDADRI--------------------KVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 356

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
           PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPME        
Sbjct: 357 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPME-------- 416

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                ACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ
Sbjct: 417 ---------------------ACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 427

[Close]

BLAST of Lsi06G009140 vs. ExPASy TrEMBL
Match: A0A5A7TKN9 (Protein argonaute PNH1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold588G00420 PE=3 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 6.1e-127
Identity = 248/300 (82.67%), Postives = 249/300 (83.00%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           EREFKVQIKFVTLASMHQLRELL GKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP
Sbjct: 230 EREFKVQIKFVTLASMHQLRELLAGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 289

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            IKKPQ+VGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS
Sbjct: 290 CIKKPQHVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 349

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           RTLSDADRI                    KVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ
Sbjct: 350 RTLSDADRI--------------------KVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 409

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
           PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPME        
Sbjct: 410 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPME-------- 469

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                ACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ
Sbjct: 470 ---------------------ACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 480

[Close]

BLAST of Lsi06G009140 vs. ExPASy TrEMBL
Match: A0A6J1KJN2 (protein argonaute PNH1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495211 PE=3 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 2.2e-121
Identity = 236/300 (78.67%), Postives = 243/300 (81.00%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           EREFKVQIKFVTLASMHQLREL  GKQV NP+EALTIIDIVLRELHAQRYISVGR FY+P
Sbjct: 178 EREFKVQIKFVTLASMHQLRELFAGKQVKNPREALTIIDIVLRELHAQRYISVGRFFYTP 237

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            IKKPQ+VGGGLQ WRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQ+LNKDVYS
Sbjct: 238 CIKKPQHVGGGLQVWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQILNKDVYS 297

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           R LSDADRI                    K+KKVLRGVKVEVTHRGNVRRKYRISGLT+Q
Sbjct: 298 RPLSDADRI--------------------KIKKVLRGVKVEVTHRGNVRRKYRISGLTTQ 357

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
           PTRELIFPLDEQMNMKSVVEYFQE+YGYTIQYTHLPCLQVGNQKKVNYLPME        
Sbjct: 358 PTRELIFPLDEQMNMKSVVEYFQEVYGYTIQYTHLPCLQVGNQKKVNYLPME-------- 417

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                ACKILKGQRYTKGLNEKQI SLLKVSCQRPSDQEMDILQ
Sbjct: 418 ---------------------ACKILKGQRYTKGLNEKQIASLLKVSCQRPSDQEMDILQ 428

[Close]

BLAST of Lsi06G009140 vs. ExPASy TrEMBL
Match: A0A6J1KJM3 (protein argonaute PNH1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495211 PE=3 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 2.2e-121
Identity = 236/300 (78.67%), Postives = 243/300 (81.00%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           EREFKVQIKFVTLASMHQLREL  GKQV NP+EALTIIDIVLRELHAQRYISVGR FY+P
Sbjct: 178 EREFKVQIKFVTLASMHQLRELFAGKQVKNPREALTIIDIVLRELHAQRYISVGRFFYTP 237

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            IKKPQ+VGGGLQ WRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQ+LNKDVYS
Sbjct: 238 CIKKPQHVGGGLQVWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQILNKDVYS 297

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           R LSDADRI                    K+KKVLRGVKVEVTHRGNVRRKYRISGLT+Q
Sbjct: 298 RPLSDADRI--------------------KIKKVLRGVKVEVTHRGNVRRKYRISGLTTQ 357

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
           PTRELIFPLDEQMNMKSVVEYFQE+YGYTIQYTHLPCLQVGNQKKVNYLPME        
Sbjct: 358 PTRELIFPLDEQMNMKSVVEYFQEVYGYTIQYTHLPCLQVGNQKKVNYLPME-------- 417

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                ACKILKGQRYTKGLNEKQI SLLKVSCQRPSDQEMDILQ
Sbjct: 418 ---------------------ACKILKGQRYTKGLNEKQIASLLKVSCQRPSDQEMDILQ 428

[Close]

BLAST of Lsi06G009140 vs. NCBI nr
Match: KAG7021819.1 (Protein argonaute PNH1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 472.2 bits (1214), Expect = 3.5e-129
Identity = 255/335 (76.12%), Postives = 263/335 (78.51%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           EREFKVQIKFVTLASMHQL+EL  GKQV NPQEALTIIDIVLRELHAQRYISVGR FYSP
Sbjct: 205 EREFKVQIKFVTLASMHQLQELFAGKQVKNPQEALTIIDIVLRELHAQRYISVGRFFYSP 264

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNI--------------------------- 122
            IKKPQ+VGGGLQ WRGFYQSIRPTQMGLSLNI                           
Sbjct: 265 CIKKPQHVGGGLQVWRGFYQSIRPTQMGLSLNIGGHRSFLFLGFYLAVFCLPELTFLMVS 324

Query: 123 --------DMSSTAFIEAIPVIDFVAQLLNKDVYSRTLSDADRIKVIISEFERSCTEQVW 182
                   DMSSTAFIEAIPVIDFVAQ+LNKDVYSR LSDADRI                
Sbjct: 325 FGNCISYPDMSSTAFIEAIPVIDFVAQILNKDVYSRPLSDADRI---------------- 384

Query: 183 MPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQPTRELIFPLDEQMNMKSVVEYFQEM 242
               K+KKVLRGVKVEVTHRGNVRRKYRISGLT+QPTRELIFPLDEQMNMKSVVEYFQE+
Sbjct: 385 ----KIKKVLRGVKVEVTHRGNVRRKYRISGLTTQPTRELIFPLDEQMNMKSVVEYFQEV 444

Query: 243 YGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTPRCLLLHLNLLSAENLRDIFPQACKI 302
           YGYTIQYTHLPCLQVGNQKKVNYLPMEVS ANY P   L   +LLSAENL D+FPQACKI
Sbjct: 445 YGYTIQYTHLPCLQVGNQKKVNYLPMEVSSANY-PSSYLKRSDLLSAENLCDVFPQACKI 504

[Close]

BLAST of Lsi06G009140 vs. NCBI nr
Match: XP_008463335.1 (PREDICTED: protein argonaute PNH1-like [Cucumis melo] >XP_008463336.1 PREDICTED: protein argonaute PNH1-like [Cucumis melo])

HSP 1 Score: 463.8 bits (1192), Expect = 1.3e-126
Identity = 248/300 (82.67%), Postives = 249/300 (83.00%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           EREFKVQIKFVTLASMHQLRELL GKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP
Sbjct: 177 EREFKVQIKFVTLASMHQLRELLAGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 236

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            IKKPQ+VGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS
Sbjct: 237 CIKKPQHVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 296

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           RTLSDADRI                    KVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ
Sbjct: 297 RTLSDADRI--------------------KVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 356

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
           PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPME        
Sbjct: 357 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPME-------- 416

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                ACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ
Sbjct: 417 ---------------------ACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 427

[Close]

BLAST of Lsi06G009140 vs. NCBI nr
Match: KAA0043378.1 (protein argonaute PNH1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 463.8 bits (1192), Expect = 1.3e-126
Identity = 248/300 (82.67%), Postives = 249/300 (83.00%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           EREFKVQIKFVTLASMHQLRELL GKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP
Sbjct: 230 EREFKVQIKFVTLASMHQLRELLAGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 289

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            IKKPQ+VGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS
Sbjct: 290 CIKKPQHVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 349

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           RTLSDADRI                    KVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ
Sbjct: 350 RTLSDADRI--------------------KVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 409

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
           PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPME        
Sbjct: 410 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPME-------- 469

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                ACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ
Sbjct: 470 ---------------------ACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 480

[Close]

BLAST of Lsi06G009140 vs. NCBI nr
Match: XP_011656603.1 (protein argonaute PNH1 isoform X1 [Cucumis sativus])

HSP 1 Score: 463.8 bits (1192), Expect = 1.3e-126
Identity = 248/300 (82.67%), Postives = 249/300 (83.00%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           EREFKVQIKFVTLASMHQLRELL GKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP
Sbjct: 203 EREFKVQIKFVTLASMHQLRELLAGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 262

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            IKKPQ+VGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS
Sbjct: 263 CIKKPQHVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 322

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           RTLSDADRI                    KVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ
Sbjct: 323 RTLSDADRI--------------------KVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 382

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
           PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPME        
Sbjct: 383 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPME-------- 442

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                ACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ
Sbjct: 443 ---------------------ACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 453

[Close]

BLAST of Lsi06G009140 vs. NCBI nr
Match: TYJ98098.1 (protein argonaute PNH1-like [Cucumis melo var. makuwa])

HSP 1 Score: 463.8 bits (1192), Expect = 1.3e-126
Identity = 248/300 (82.67%), Postives = 249/300 (83.00%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           EREFKVQIKFVTLASMHQLRELL GKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP
Sbjct: 177 EREFKVQIKFVTLASMHQLRELLAGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 236

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            IKKPQ+VGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS
Sbjct: 237 CIKKPQHVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 296

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           RTLSDADRI                    KVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ
Sbjct: 297 RTLSDADRI--------------------KVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 356

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
           PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPME        
Sbjct: 357 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPME-------- 416

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                ACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ
Sbjct: 417 ---------------------ACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 427

[Close]

BLAST of Lsi06G009140 vs. TAIR 10
Match: AT5G43810.1 (Stabilizer of iron transporter SufD / Polynucleotidyl transferase )

HSP 1 Score: 350.1 bits (897), Expect = 1.9e-96
Identity = 183/300 (61.00%), Postives = 217/300 (72.33%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           ER +KV IKFV  A+MH L E L GK+ + PQEA+ I+DIVLREL  +R+  VGRSF+SP
Sbjct: 234 ERSYKVAIKFVARANMHHLGEFLAGKRADCPQEAVQILDIVLRELSVKRFCPVGRSFFSP 293

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            IK PQ +G GL++W GFYQSIRPTQMGLSLNIDM+S AFIE +PVI+FVAQLL KDV S
Sbjct: 294 DIKTPQRLGEGLESWCGFYQSIRPTQMGLSLNIDMASAAFIEPLPVIEFVAQLLGKDVLS 353

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           + LSD+DR+                    K+KK LRGVKVEVTHR NVRRKYR++GLT+Q
Sbjct: 354 KPLSDSDRV--------------------KIKKGLRGVKVEVTHRANVRRKYRVAGLTTQ 413

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
           PTREL+FP+DE   MKSV+EYFQEMYG+TIQ+THLPCLQVGNQKK +YLPME        
Sbjct: 414 PTRELMFPVDENCTMKSVIEYFQEMYGFTIQHTHLPCLQVGNQKKASYLPME-------- 473

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                ACKI++GQRYTK LNEKQIT+LLKV+CQRP D+E DIL+
Sbjct: 474 ---------------------ACKIVEGQRYTKRLNEKQITALLKVTCQRPRDRENDILR 484

[Close]

BLAST of Lsi06G009140 vs. TAIR 10
Match: AT5G43810.2 (Stabilizer of iron transporter SufD / Polynucleotidyl transferase )

HSP 1 Score: 350.1 bits (897), Expect = 1.9e-96
Identity = 183/300 (61.00%), Postives = 217/300 (72.33%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           ER +KV IKFV  A+MH L E L GK+ + PQEA+ I+DIVLREL  +R+  VGRSF+SP
Sbjct: 234 ERSYKVAIKFVARANMHHLGEFLAGKRADCPQEAVQILDIVLRELSVKRFCPVGRSFFSP 293

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            IK PQ +G GL++W GFYQSIRPTQMGLSLNIDM+S AFIE +PVI+FVAQLL KDV S
Sbjct: 294 DIKTPQRLGEGLESWCGFYQSIRPTQMGLSLNIDMASAAFIEPLPVIEFVAQLLGKDVLS 353

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           + LSD+DR+                    K+KK LRGVKVEVTHR NVRRKYR++GLT+Q
Sbjct: 354 KPLSDSDRV--------------------KIKKGLRGVKVEVTHRANVRRKYRVAGLTTQ 413

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
           PTREL+FP+DE   MKSV+EYFQEMYG+TIQ+THLPCLQVGNQKK +YLPME        
Sbjct: 414 PTRELMFPVDENCTMKSVIEYFQEMYGFTIQHTHLPCLQVGNQKKASYLPME-------- 473

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                ACKI++GQRYTK LNEKQIT+LLKV+CQRP D+E DIL+
Sbjct: 474 ---------------------ACKIVEGQRYTKRLNEKQITALLKVTCQRPRDRENDILR 484

[Close]

BLAST of Lsi06G009140 vs. TAIR 10
Match: AT1G48410.1 (Stabilizer of iron transporter SufD / Polynucleotidyl transferase )

HSP 1 Score: 335.1 bits (858), Expect = 6.3e-92
Identity = 181/300 (60.33%), Postives = 207/300 (69.00%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQRYISVGRSFYSP 62
           EREFKV IK V  A +H L   L GKQ + PQEAL ++DIVLREL   RYI VGRSFYSP
Sbjct: 284 EREFKVVIKLVARADLHHLGMFLEGKQSDAPQEALQVLDIVLRELPTSRYIPVGRSFYSP 343

Query: 63  YIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDVYS 122
            I K Q++G GL++WRGFYQSIRPTQMGLSLNIDMSSTAFIEA PVI FV  LLN+D+ S
Sbjct: 344 DIGKKQSLGDGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEANPVIQFVCDLLNRDISS 403

Query: 123 RTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLTSQ 182
           R LSDADR+                    K+KK LRGVKVEVTHRGN+RRKYRISGLT+ 
Sbjct: 404 RPLSDADRV--------------------KIKKALRGVKVEVTHRGNMRRKYRISGLTAV 463

Query: 183 PTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANYTP 242
            TREL FP+DE+   KSVVEYF E YG+ IQ+T LPCLQVGN  + NYLPMEV       
Sbjct: 464 ATRELTFPVDERNTQKSVVEYFHETYGFRIQHTQLPCLQVGNSNRPNYLPMEV------- 523

Query: 243 RCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDILQ 302
                                 CKI++GQRY+K LNE+QIT+LLKV+CQRP D+E DILQ
Sbjct: 524 ----------------------CKIVEGQRYSKRLNERQITALLKVTCQRPIDREKDILQ 534

[Close]

BLAST of Lsi06G009140 vs. TAIR 10
Match: AT1G48410.2 (Stabilizer of iron transporter SufD / Polynucleotidyl transferase )

HSP 1 Score: 330.1 bits (845), Expect = 2.0e-90
Identity = 181/302 (59.93%), Postives = 207/302 (68.54%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQ--RYISVGRSFY 62
           EREFKV IK V  A +H L   L GKQ + PQEAL ++DIVLREL     RYI VGRSFY
Sbjct: 284 EREFKVVIKLVARADLHHLGMFLEGKQSDAPQEALQVLDIVLRELPTSRIRYIPVGRSFY 343

Query: 63  SPYIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDV 122
           SP I K Q++G GL++WRGFYQSIRPTQMGLSLNIDMSSTAFIEA PVI FV  LLN+D+
Sbjct: 344 SPDIGKKQSLGDGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEANPVIQFVCDLLNRDI 403

Query: 123 YSRTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLT 182
            SR LSDADR+                    K+KK LRGVKVEVTHRGN+RRKYRISGLT
Sbjct: 404 SSRPLSDADRV--------------------KIKKALRGVKVEVTHRGNMRRKYRISGLT 463

Query: 183 SQPTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANY 242
           +  TREL FP+DE+   KSVVEYF E YG+ IQ+T LPCLQVGN  + NYLPMEV     
Sbjct: 464 AVATRELTFPVDERNTQKSVVEYFHETYGFRIQHTQLPCLQVGNSNRPNYLPMEV----- 523

Query: 243 TPRCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDI 302
                                   CKI++GQRY+K LNE+QIT+LLKV+CQRP D+E DI
Sbjct: 524 ------------------------CKIVEGQRYSKRLNERQITALLKVTCQRPIDREKDI 536

[Close]

BLAST of Lsi06G009140 vs. TAIR 10
Match: AT1G48410.3 (Stabilizer of iron transporter SufD / Polynucleotidyl transferase )

HSP 1 Score: 330.1 bits (845), Expect = 2.0e-90
Identity = 181/302 (59.93%), Postives = 207/302 (68.54%), Query Frame = 0


Query: 3   EREFKVQIKFVTLASMHQLRELLTGKQVNNPQEALTIIDIVLRELHAQ--RYISVGRSFY 62
           EREFKV IK V  A +H L   L GKQ + PQEAL ++DIVLREL     RYI VGRSFY
Sbjct: 284 EREFKVVIKLVARADLHHLGMFLEGKQSDAPQEALQVLDIVLRELPTSRIRYIPVGRSFY 343

Query: 63  SPYIKKPQNVGGGLQAWRGFYQSIRPTQMGLSLNIDMSSTAFIEAIPVIDFVAQLLNKDV 122
           SP I K Q++G GL++WRGFYQSIRPTQMGLSLNIDMSSTAFIEA PVI FV  LLN+D+
Sbjct: 344 SPDIGKKQSLGDGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEANPVIQFVCDLLNRDI 403

Query: 123 YSRTLSDADRIKVIISEFERSCTEQVWMPFCKVKKVLRGVKVEVTHRGNVRRKYRISGLT 182
            SR LSDADR+                    K+KK LRGVKVEVTHRGN+RRKYRISGLT
Sbjct: 404 SSRPLSDADRV--------------------KIKKALRGVKVEVTHRGNMRRKYRISGLT 463

Query: 183 SQPTRELIFPLDEQMNMKSVVEYFQEMYGYTIQYTHLPCLQVGNQKKVNYLPMEVSPANY 242
           +  TREL FP+DE+   KSVVEYF E YG+ IQ+T LPCLQVGN  + NYLPMEV     
Sbjct: 464 AVATRELTFPVDERNTQKSVVEYFHETYGFRIQHTQLPCLQVGNSNRPNYLPMEV----- 523

Query: 243 TPRCLLLHLNLLSAENLRDIFPQACKILKGQRYTKGLNEKQITSLLKVSCQRPSDQEMDI 302
                                   CKI++GQRY+K LNE+QIT+LLKV+CQRP D+E DI
Sbjct: 524 ------------------------CKIVEGQRYSKRLNERQITALLKVTCQRPIDREKDI 536

The following BLAST results are available for this feature:

BLAST of Lsi06G009140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q69VD5	2.9e-102	64.33	Protein argonaute PNH1 OS=Oryza sativa subsp. japonica OX=39947 GN=PHN1 PE=2 SV=...	[more]
Q9XGW1	2.7e-95	61.00	Protein argonaute 10 OS=Arabidopsis thaliana OX=3702 GN=AGO10 PE=1 SV=1	[more]
O04379	8.8e-91	60.33	Protein argonaute 1 OS=Arabidopsis thaliana OX=3702 GN=AGO1 PE=1 SV=1	[more]
Q6EU14	4.4e-90	58.67	Protein argonaute 1A OS=Oryza sativa subsp. japonica OX=39947 GN=AGO1A PE=2 SV=1	[more]
Q7XSA2	2.2e-89	58.00	Protein argonaute 1B OS=Oryza sativa subsp. japonica OX=39947 GN=AGO1B PE=2 SV=3	[more]

BLAST of Lsi06G009140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CJ14	6.1e-127	82.67	protein argonaute PNH1-like OS=Cucumis melo OX=3656 GN=LOC103501520 PE=3 SV=1	[more]
A0A5D3BIT5	6.1e-127	82.67	Protein argonaute PNH1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A5A7TKN9	6.1e-127	82.67	Protein argonaute PNH1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A6J1KJN2	2.2e-121	78.67	protein argonaute PNH1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114952...	[more]
A0A6J1KJM3	2.2e-121	78.67	protein argonaute PNH1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114952...	[more]

BLAST of Lsi06G009140 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021819.1	3.5e-129	76.12	Protein argonaute PNH1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_008463335.1	1.3e-126	82.67	PREDICTED: protein argonaute PNH1-like [Cucumis melo] >XP_008463336.1 PREDICTED:...	[more]
KAA0043378.1	1.3e-126	82.67	protein argonaute PNH1 isoform X1 [Cucumis melo var. makuwa]	[more]
XP_011656603.1	1.3e-126	82.67	protein argonaute PNH1 isoform X1 [Cucumis sativus]	[more]
TYJ98098.1	1.3e-126	82.67	protein argonaute PNH1-like [Cucumis melo var. makuwa]	[more]

BLAST of Lsi06G009140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43810.1	1.9e-96	61.00	Stabilizer of iron transporter SufD / Polynucleotidyl transferase	[more]
AT5G43810.2	1.9e-96	61.00	Stabilizer of iron transporter SufD / Polynucleotidyl transferase	[more]
AT1G48410.1	6.3e-92	60.33	Stabilizer of iron transporter SufD / Polynucleotidyl transferase	[more]
AT1G48410.2	2.0e-90	59.93	Stabilizer of iron transporter SufD / Polynucleotidyl transferase	[more]
AT1G48410.3	2.0e-90	59.93	Stabilizer of iron transporter SufD / Polynucleotidyl transferase	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014811	Argonaute, linker 1 domain	SMART	SM01163	DUF1785_2	coord: 53..105 e-value: 6.3E-26 score: 102.1
IPR014811	Argonaute, linker 1 domain	PFAM	PF08699	ArgoL1	coord: 54..104 e-value: 8.2E-22 score: 76.6
IPR003100	PAZ domain	SMART	SM00949	PAZ_2_a_3	coord: 129..254 e-value: 0.0067 score: 2.1
IPR003100	PAZ domain	PFAM	PF02170	PAZ	coord: 141..235 e-value: 1.1E-19 score: 70.7
IPR003100	PAZ domain	PROSITE	PS50821	PAZ	coord: 152..235 score: 26.778601
None	No IPR available	GENE3D	2.170.260.10	paz domain	coord: 99..237 e-value: 1.0E-31 score: 111.7
None	No IPR available	PANTHER	PTHR22891:SF122	PROTEIN ARGONAUTE 10	coord: 151..236
None	No IPR available	PANTHER	PTHR22891:SF122	PROTEIN ARGONAUTE 10	coord: 3..133 coord: 263..304
None	No IPR available	PANTHER	PTHR22891	EUKARYOTIC TRANSLATION INITIATION FACTOR 2C	coord: 3..133 coord: 151..236
None	No IPR available	PANTHER	PTHR22891	EUKARYOTIC TRANSLATION INITIATION FACTOR 2C	coord: 263..304
None	No IPR available	CDD	cd02846	PAZ_argonaute_like	coord: 105..235 e-value: 3.01147E-36 score: 124.737
IPR036085	PAZ domain superfamily	SUPERFAMILY	101690	PAZ domain	coord: 100..303

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G009140.1	Lsi06G009140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051607	defense response to virus
biological_process	GO:0031047	gene silencing by RNA
biological_process	GO:0006955	immune response
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0005515	protein binding