Lsi06G009000 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G009000
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionNudix hydrolase 20
Locationchr06: 18609229 .. 18614969 (-)
RNA-Seq ExpressionLsi06G009000
SyntenyLsi06G009000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGGACCTTTCACATGCTGATTTGTCCCAAAACTCATCCTTTTGAAAAAGCATCCCAAAATTCTTGGCTATCTGCTCTGGTAATTCTGGCGACGTTTGCTCCAATCCAATTCCATGGCCTGCAAATCTCTCGCTACACACAGCTTTCGTAACTTCTCCACTGTCTCTTCCTTCTGCAATGTTTCAACAATTTCCCGGACGACGTATCCAATGACGTCTACATTTCGGACAACAATACACCGCTCGATATCGGTCGCCTCTCCTTCTGTATCGGTCACCGCCGGCAGTTTCACTTGGGATGACGTTGTTCACGTTTCCTTACCAAAAAGCTTTCAAGACGATCCTTCAGATCTGACGGGCTATTTCGAGAAGGTCAAGCTCTGCAATCTCGGATCAGTAGGTCTCTTTCTCCTTTTCTTTTTTGTTTCGCTGTATCAAACGAAAGACGTAAAATCATTCATTCATGTAGTACTTGTACGCGTTAGTTCGTTTTACATTTTAAATTCAGTGATGGAAAAGCTATAGACTGACGGAATGTTGTTCCAAATTTTCTTACAGGATACGCAATCTGAATTTCTTCCGTTTGTCATCGATGACCAAATTGTTGGCTATGTGCACCACGGGTGAGGCTCCTGTGTTATATCTTTCTGGATAACACTATGTACATTTAAAATATGATCCATCTGTGTTGCCAATGCCATTTATGTGTACTCATTTCATCAAGTTTAGCTTGTGTGCAGTTGCTATATACAATGGATAGGTTCAATTGGTTTGTTTTAATGTACTTTATGCAGTTTCGCTAAACACCTGAAGCAGTATGCGAATGTGTTTGCCTTCCCCCGAGACGATTCTGTTAAATTTGGCGCCTACTTAACCTTGCATGAATCGTTGAAGACACCCAAAGATAGGACGCTTGCAGTTGGAGATGTAGTTAAATGCTTGGGTGAAGAAGTCATTCCAGGTACACGGAATGAGGTACTGCTTTGTGATTCCAATTCAAAGCAATTATTACATTACTTTTTAATACCTGTTTGCAACCGTTGCATTGAAAGAAAAAAAAGGAAAAAAGAATTGCCTTCTTATGTTTAATGCTCGAAATAAACTGGTCCTTTTTAAAAGGTAAATTTAGAAGAAGGCAATACTTCTTGTGGCTAACTGTGGATAACCTTGAATTCTATCAGCTATACCCTGTTACTTCGTCCTTTGGTGCCCCGAACTTTTTCTCACTCGAACGTGCTGCAGCCCCTTATTTTGGAATAAAGGTGCGAATGTTCCCCTTTTACTGCTTTGTGGAAACTTTTAAAGCTTTGTTCTAATTTTGTGAAATGCACTTCGGAAGCTATATTTTGATCCTGCTTTTTGTTAGGCCCTTGAGAATAGCAGGCCATTTGTTTTGTTGAATCTGTTTGCTCAAGTTTGGTTGTTAGGTCCAAATAAAACTGTAACAAGTTATATTTATAGTCATTGACTCATTCTGAATACAAAGATTTGAAGTAACTGAAGATTTTGCAAACAACTTTATGGTGCTGAAAGAATTTTACTTGGCAACTGCATTTCTTTTACACGTGCCAATCTCCTACCTTGATCAGAGGTTCAATTGTGGAATGTGACACACAAGCACGCACACACAAATGTATAAAAGATGTATGGGGAAGTGGTATAAGAGAGAATTTTAATGTTGGGAATATTGGGGAATGGATTTCTTTACTTAAATTGATGACTTCACTCCTTGCATGATGAGAGGATTGTAGGGTTTGGGATTGAAACCTGGATAAATCTAGAACCTTTCTTGAGATTCTATGATGGATCATCTTACCACCATGGTGGATACCTGTCCTGCAATGTTTGCCAACAATAAACTATGATGGAAATTTATTTTTCCAAAAGGATCAGATTTGTCTTATGCTCCCTAGCTCAGCCAAAGTTCATTCTACCAACCGTACCTAAAAGAAAACTTTCTGCTTTTATCTCCTCAATGCTATGGTTCACTAGAAAATGATTGGGACATGCCTCAAAGTAGCTAGAGAGGGCATTTTCTTAATATTACTTTTGCAGGTTAAGTGAAACTTTTTGAAGGGTTATATAGAATCTCTCTCAGTGCTATGGTGTAAATAGAAAATTCCTTTTGATTAGGGTAAAGCATACAGCACTATAGCTGCTCTCTCAAGTTGTTCATACCCTTCTGATATACTTACAAAAGAAGAGCCTAGACATTGAAGGGAAATTTATTGATGAATGTGCTTCTCGGAGACGGAAACTGGCTGCTTGGTTGAAGATAACTGAAAGTTATATTATGCCACTACTTGGTTTCTCTAACTTTTTTCTGCCTTCTCTTGGCGCTTTTCTTAGCACAGGTTTATGGAGTTCATATGAATGGTTATGTTGAGAAGGAAGGAAAAAAGTTTTTGTGGGTTGCGAAAAGAAGTCAAACGAAGCCAACTTTTCCTGGAATGTTGGATCATTTAGTAGCTGGAGGGCTGGTACGTAATAAGTAATAGCTGTTGCAAGTTTCTTTACATGTGCTTATGTCCTGTTAAAATGTGAAAAATAAGGACTATTCTGCTTTTCTTCGTGATTGGATGGTATGGTGTGTTAGTTCCCTTAACCTTTTCCGTTTCTTCTGCTAGATGTTTGTAGTTATATAGATACTTTCCAGTAGGTTAATATATCATTTAGCCTTCAAATCCTTTTTCAATTTGGTTCATAATATATTCAAAGTTGTAAGGGTTAATTTTACCCTTGAGATATCAAAATATTTCAATGAAGTTCATATTATTCGTTGAAATTGAAAAAGAATGGTGACCTGAAATTTAAAATATTATTTTCTATGAGCAAAGGTCTTTTGAATGTTAAATATCCATCTTATCTTATTTAGCCAAAAATCCGACCTCCCTATATATAGTAAGATAAGATATATTTACCCTTGTTTTATTTAATTTCATTGACTTTATTGAATATGGACTTGATTGAACATTCTGATTCCTTGAGAGATGTAATCTCCAGAGGCTGAATTATATATTTAACCTTTCTGCAAGTATTACATCCTGGCTCCTGCTATAAGGAATCAGAATCTGTCTGTGATTCAGCTAATATGCATGTCTAGTTTCTTAAAGTTAGTTCACGCATACAACAATTATTGATCTTTACTTTGTCTGACCGTCCATTTGGACAATAAAGTTTAAAAGTTAATAAAAAAATTAGACAAGGTAAATACTACAAACTTTTTGACGATCTACAACTTATTAATAAATTATTGGTTGAGCAGTGTGTTATATTGAGTTGAGACTGGATGTGAAATATGATGATTTTCACCAGAGCAGTTAGAAAATCTATGCCTATGGATATAAAAATGTAGTTGTACATGCAAACCTTGATACTAATCAGCAGAAAAAGAGACAAGGGAAATGCAAAGTTTGAAATGTGCCCTTGAATGCTGAATGCCATATTTATTGTGCCTTTGTAGCTAGCCCCTGACATTATGATTGTGACAGCCTCAGGGCATCGCTTGTGGGGAGAATCTGATGAAAGAATGTGAGGAGGAAGCAGGCATACCAAGATCGATATCAAAAGAGTAATTTCCTAACAAGAATTTAGTTATAATTTCACACGAAGATAACCTCCGTGGATTACATGGTTGTTGTGGACCTAGTGGTTTCACATGATGCTTTTGTCTTAGGGCCATACCAGTTGGTGCCATTTCATACACAGACATCAAAGGTTATGGTTACAAGAGAGATGTTCAATTCTGTTACGATTTAAAACTTCCCGAAAGCTTTATACCGGAGAATCAAGGTTAGCTAGTCTGATAATTTTTTGTTTCCATTCCAATTCAATATTTAGCTTACAAGGTATGGTACATTGCATTTTACTGGCTCTTAACAACTCTTTTTTGTCCGAGCCCCAATGTACATATTCTGCATGCATAATTTTCATTACAAATTCTGTATAGTTTGAAATCTCATGTCACAAGCTTGCTGACTGTACTTTTGTTAGATGGGGAGGTTGAAGGATTCATGTTGTTGCCTGTGACAAATGTTGCAAATGTGATTCGGAGGACACAGTTATTCAAGCCTAATTGTTCTCTCGTCATTATCAATTTCCTATTTCGACATGGGTACTATATACTATCTTCTCTGAATATACTTTGTTCCTCGACCATTATTTGATTTAATTACAACAATAATGTAGGTTTTGGTTGTAAATATTTGTTGGTTCAACCAAAAATTCTCATGAGTACAATTTTTTCTAGACCACATAGGATGTATTTGATGGGTGGTCTAACAAGGAGACACAATGGAAAATAATTATATTCCACGCTTTCATAATTTTTTAATACGTGGTTGTTCACTTTGTTTTTAGACGAGCAGATTTTGATTCTGAAAACAATGAAAATACATAAATGTTTTGAAAAGATTCACTTTCTATGTCATAGAATTTAAACACAATTCCAATAGTGTTTTTCATAAACAAGTCCACCATATAGAAATTAATTACTGAGCTCATTTGATTCAGTAAATTTGAAGAAAATAAAATGAATTTAGATAAGATACCAAATAATTTTTTATAAATGTCGATTACAAAAACACTGTTAAGACAGGTAGATTGGAGATCATTTTTGTCTCCAGGTTCTGATCAGTTTAGTTCTAAACCTTTTTATCCATGTGTGTAACCAACCCCAGTATAGGTTAATTACCACTCCTTCTCAAGGTATCCTTAAAAAGTCTACAGTTCTTTGTATTAATGCATTTAAAAACTTGCAGGTACATTAAGCCCGAAAGTTTGGGGTACTTGGAGCTACTGCAGAGTTTGAGAAGTGGGACATGCTTCTGAATAAAAAAATTCGCCCTATGCATTGATGAGAGAAATCAGGTCAATCTTATAAAACATTGAATCTGGATGGTATATATATTGTTTATGGTTTTCCCAGAACTTTCTTGCTCATTAAATGTATTTGTGAAGCCAAACAAACTGCCTTACACTTGGCTATAGAGAATAAAATAAGAAGACTAAGTTTTGGTACATCTATTGAATGAAAACACCTTAACAAAATTACACAAAGCCCATTACATATAAAACAATCTTAATTTCATCAACTCCCCAAGCAACTATAAACACACTACACTTCACACTGACTTTTACATTATTCACTTCCTTTTCTAACCCATTATATTTCACTAAGATCTATTGAATGTCAAAAGCCCAAGACTTCACCAAGAACTTGAAGCAACTCGTCGTGTGCTGTTAAGTGAGGGAAGTGTCCATTAGTGTTAATAACTCGCACTGTGCAACTTCCTTTGATTTTCTTCTGCATAAAGATGGGAACTGAGGCAGGGACGACAATGTCGGTTTTCGTTTGCAGGATGATGCATGGAATGTCTACCTTCTCAAGAATATCTCTCTCATCTACACTGAACACAGTCCTGGCCAAAGGTACTGCAACTTCAGGCCTCATCTCTTGCAAACATTTCTGAAACCTGCTGATTGACAGAGGATCGGCTTCGTCCACAACCAGAGAAGCAAAGTTTGTCGCCCAATTTTCATAGTTGGATTCCATATTTGCTATGATTTGATCGATGTCCGATTTGTTGAAACCACCTTCATAGTCTTCTGTGTTTATATACCTAACAACACAATGGTCAATGTGTTAGAAATAACATGTTCCAACTTTCCAGGGCCTCCTTTTACTGTGTTATGCTAGATCGATCTCTCTCTAAATCAAACCATTCTAATTTGTGTTAAAT

mRNA sequence

TTTGGACCTTTCACATGCTGATTTGTCCCAAAACTCATCCTTTTGAAAAAGCATCCCAAAATTCTTGGCTATCTGCTCTGGTAATTCTGGCGACGTTTGCTCCAATCCAATTCCATGGCCTGCAAATCTCTCGCTACACACAGCTTTCGTAACTTCTCCACTGTCTCTTCCTTCTGCAATGTTTCAACAATTTCCCGGACGACGTATCCAATGACGTCTACATTTCGGACAACAATACACCGCTCGATATCGGTCGCCTCTCCTTCTGTATCGGTCACCGCCGGCAGTTTCACTTGGGATGACGTTGTTCACGTTTCCTTACCAAAAAGCTTTCAAGACGATCCTTCAGATCTGACGGGCTATTTCGAGAAGGTCAAGCTCTGCAATCTCGGATCAGATACGCAATCTGAATTTCTTCCGTTTGTCATCGATGACCAAATTGTTGGCTATGTGCACCACGGTTTCGCTAAACACCTGAAGCAGTATGCGAATGTGTTTGCCTTCCCCCGAGACGATTCTGTTAAATTTGGCGCCTACTTAACCTTGCATGAATCGTTGAAGACACCCAAAGATAGGACGCTTGCAGTTGGAGATGTAGTTAAATGCTTGGGTGAAGAAGTCATTCCAGGTACACGGAATGAGCTATACCCTGTTACTTCGTCCTTTGGTGCCCCGAACTTTTTCTCACTCGAACGTGCTGCAGCCCCTTATTTTGGAATAAAGGCCCTTGAGAATAGCAGGCCATTTGTTTTGTTGAATCTATTTGAAGTAACTGAAGATTTTGCAAACAACTTTATGGTGCTGAAAGAATTTTACTTGGCAACTGCATTTCTTTTACACGTGCCAATCTCCTACCTTGATCAGAGGTTAACACAGGTTTATGGAGTTCATATGAATGGTTATGTTGAGAAGGAAGGAAAAAAGTTTTTGTGGGTTGCGAAAAGAAGTCAAACGAAGCCAACTTTTCCTGGAATGTTGGATCATTTAGTAGCTGGAGGGCTGGTACTTCCCTTAACCTTTTCCGTTTCTTCTGCTAGATTTGTACATGCAAACCTTGATACTAATCAGCAGAAAAAGAGACAAGGGAAATGCAAACCTCAGGGCATCGCTTGTGGGGAGAATCTGATGAAAGAATGTGAGGAGGAAGCAGGCATACCAAGATCGATATCAAAAGAGGCCATACCAGTTGGTGCCATTTCATACACAGACATCAAAGGTTATGGTTACAAGAGAGATGTTCAATTCTGTTACGATTTAAAACTTCCCGAAAGCTTTATACCGGAGAATCAAGATGGGGAGGTTGAAGGATTCATGTTGTTGCCTGTGACAAATGTTGCAAATGTGATTCGGAGGACACAGTTATTCAAGCCTAATTGTTCTCTCGTCATTATCAATTTCCTATTTCGACATGGGTACATTAAGCCCGAAAGTTTGGGGTACTTGGAGCTACTGCAGAGTTTGAGAAGTGGGACATGCTTCTGAATAAAAAAATTCGCCCTATGCATTGATGAGAGAAATCAGGATGATGCATGGAATGTCTACCTTCTCAAGAATATCTCTCTCATCTACACTGAACACAGTCCTGGCCAAAGGTACTGCAACTTCAGGCCTCATCTCTTGCAAACATTTCTGAAACCTGCTGATTGACAGAGGATCGGCTTCGTCCACAACCAGAGAAGCAAAGTTTGTCGCCCAATTTTCATAGTTGGATTCCATATTTGCTATGATTTGATCGATGTCCGATTTGTTGAAACCACCTTCATAGTCTTCTGTGTTTATATACCTAACAACACAATGGTCAATGTGTTAGAAATAACATGTTCCAACTTTCCAGGGCCTCCTTTTACTGTGTTATGCTAGATCGATCTCTCTCTAAATCAAACCATTCTAATTTGTGTTAAAT

Coding sequence (CDS)

ATGGCCTGCAAATCTCTCGCTACACACAGCTTTCGTAACTTCTCCACTGTCTCTTCCTTCTGCAATGTTTCAACAATTTCCCGGACGACGTATCCAATGACGTCTACATTTCGGACAACAATACACCGCTCGATATCGGTCGCCTCTCCTTCTGTATCGGTCACCGCCGGCAGTTTCACTTGGGATGACGTTGTTCACGTTTCCTTACCAAAAAGCTTTCAAGACGATCCTTCAGATCTGACGGGCTATTTCGAGAAGGTCAAGCTCTGCAATCTCGGATCAGATACGCAATCTGAATTTCTTCCGTTTGTCATCGATGACCAAATTGTTGGCTATGTGCACCACGGTTTCGCTAAACACCTGAAGCAGTATGCGAATGTGTTTGCCTTCCCCCGAGACGATTCTGTTAAATTTGGCGCCTACTTAACCTTGCATGAATCGTTGAAGACACCCAAAGATAGGACGCTTGCAGTTGGAGATGTAGTTAAATGCTTGGGTGAAGAAGTCATTCCAGGTACACGGAATGAGCTATACCCTGTTACTTCGTCCTTTGGTGCCCCGAACTTTTTCTCACTCGAACGTGCTGCAGCCCCTTATTTTGGAATAAAGGCCCTTGAGAATAGCAGGCCATTTGTTTTGTTGAATCTATTTGAAGTAACTGAAGATTTTGCAAACAACTTTATGGTGCTGAAAGAATTTTACTTGGCAACTGCATTTCTTTTACACGTGCCAATCTCCTACCTTGATCAGAGGTTAACACAGGTTTATGGAGTTCATATGAATGGTTATGTTGAGAAGGAAGGAAAAAAGTTTTTGTGGGTTGCGAAAAGAAGTCAAACGAAGCCAACTTTTCCTGGAATGTTGGATCATTTAGTAGCTGGAGGGCTGGTACTTCCCTTAACCTTTTCCGTTTCTTCTGCTAGATTTGTACATGCAAACCTTGATACTAATCAGCAGAAAAAGAGACAAGGGAAATGCAAACCTCAGGGCATCGCTTGTGGGGAGAATCTGATGAAAGAATGTGAGGAGGAAGCAGGCATACCAAGATCGATATCAAAAGAGGCCATACCAGTTGGTGCCATTTCATACACAGACATCAAAGGTTATGGTTACAAGAGAGATGTTCAATTCTGTTACGATTTAAAACTTCCCGAAAGCTTTATACCGGAGAATCAAGATGGGGAGGTTGAAGGATTCATGTTGTTGCCTGTGACAAATGTTGCAAATGTGATTCGGAGGACACAGTTATTCAAGCCTAATTGTTCTCTCGTCATTATCAATTTCCTATTTCGACATGGGTACATTAAGCCCGAAAGTTTGGGGTACTTGGAGCTACTGCAGAGTTTGAGAAGTGGGACATGCTTCTGA

Protein sequence

MACKSLATHSFRNFSTVSSFCNVSTISRTTYPMTSTFRTTIHRSISVASPSVSVTAGSFTWDDVVHVSLPKSFQDDPSDLTGYFEKVKLCNLGSDTQSEFLPFVIDDQIVGYVHHGFAKHLKQYANVFAFPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGEEVIPGTRNELYPVTSSFGAPNFFSLERAAAPYFGIKALENSRPFVLLNLFEVTEDFANNFMVLKEFYLATAFLLHVPISYLDQRLTQVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLVLPLTFSVSSARFVHANLDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPNCSLVIINFLFRHGYIKPESLGYLELLQSLRSGTCF
Homology
BLAST of Lsi06G009000 vs. ExPASy Swiss-Prot
Match: Q8VXZ0 (Nudix hydrolase 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT20 PE=2 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 5.3e-102
Identity = 202/448 (45.09%), Postives = 268/448 (59.82%), Query Frame = 0

Query: 15  STVSSFCNVSTISRTTYPM-----TSTFRTTIHRSISVASPSVSVTAGSFTWDDVVHVSL 74
           +  +S  +   I+R   P+     +S   + +  S ++++ +    + SFTWDDV+    
Sbjct: 11  TVTTSLFSSHAITRRVLPILRWRSSSMSLSPLRHSRALSAATTVPISSSFTWDDVIETG- 70

Query: 75  PKSFQDDPSDLTGYFEKVKLCNLGSDTQSEFLPFVIDDQIVGYVHHGFAKHLKQYANVFA 134
               + + SDLTG+FEK+  CN GS+   EF+PFVI++QIVGY+H  F ++L+++ ++F 
Sbjct: 71  --RAEYNSSDLTGFFEKINRCNRGSEKLGEFIPFVIEEQIVGYIHKRFTEYLREFHDIFT 130

Query: 135 FPRDDSV--KFGAYLTLHESLKTPKDRTLAVGDVVKCLGEE-VIPGTRNELYPVTSSFGA 194
           F ++ S   +   Y+TL+  L+ P+DRT AV DV+K LG++ +IPG RNELYPV  SF A
Sbjct: 131 FSQNGSCPDRVDGYVTLNLMLQKPEDRTRAVADVIKILGDKGIIPGIRNELYPVKPSFNA 190

Query: 195 PNFFSLERAAAPYFGIKALENSRPFVLLNLFEVTEDFANNFMVLKEFYLATAFLLHVPIS 254
           P FFSLERAAAPYFGIK                                           
Sbjct: 191 PVFFSLERAAAPYFGIKG------------------------------------------ 250

Query: 255 YLDQRLTQVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLVLPLTFSVSS 314
                    YGVHMNGYVE++G+K LW+ KRS +K T+PGMLDHLVAGGL          
Sbjct: 251 ---------YGVHMNGYVERDGQKLLWIGKRSLSKSTYPGMLDHLVAGGL---------- 310

Query: 315 ARFVHANLDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIPVGAISYTDI 374
                                P GI+CG NL+KECEEEAGI R+I+  AI VGA+SY DI
Sbjct: 311 ---------------------PHGISCGGNLVKECEEEAGISRAIADRAIAVGAVSYLDI 370

Query: 375 KGYGYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPNCSLVII 434
             Y +KRDV FCYDL+LPE F+P+NQDGEVE F L+PV  VA+VI++T  FK NCSLVII
Sbjct: 371 DQYCFKRDVLFCYDLELPEDFVPKNQDGEVESFKLIPVAQVASVIKKTSFFKANCSLVII 373

Query: 435 NFLFRHGYIKPESLGYLELLQSLRSGTC 455
           +FLFRHG+I+PES GYL+L Q LR+  C
Sbjct: 431 DFLFRHGFIRPESSGYLDLYQRLRNRDC 373

BLAST of Lsi06G009000 vs. ExPASy Swiss-Prot
Match: P0C026 (Nudix hydrolase 24, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT24 PE=2 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 3.1e-94
Identity = 191/449 (42.54%), Postives = 255/449 (56.79%), Query Frame = 0

Query: 18  SSFCNVSTISRTTYPMTSTFRTTIHRSISVA-SPSVSVT----------AGSFTWDDVVH 77
           S+FC++     + +   +   T   RS S + SP + ++          + SFTW+DV  
Sbjct: 3   SAFCSLCPTPTSLFSSHALIPTLQWRSSSSSRSPPLHISRVLSVETVPLSPSFTWNDVFE 62

Query: 78  VSLPKSFQDDPSDLTGYFEKVKLCNLGSDTQSEFLPFVIDDQIVGYVHHGFAKHLKQYAN 137
            S  +    + SDLTG+ EKV  CN G +   EF+PFVI++QIVGY+H GF K+L+ + +
Sbjct: 63  NSRKEYVPQNSSDLTGFLEKVDRCNRGLEKLGEFIPFVIEEQIVGYIHKGFTKYLRDFND 122

Query: 138 VFAFPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGEE-VIPGTRNELYPVTSSFG 197
           +F F      ++G ++TL+  L  P++RT AV  V+K LG + +IPG RNELYPV  SF 
Sbjct: 123 IFTFS-----QYGGHVTLNMMLDKPEERTRAVAHVIKILGNKGIIPGIRNELYPVKPSFN 182

Query: 198 APNFFSLERAAAPYFGIKALENSRPFVLLNLFEVTEDFANNFMVLKEFYLATAFLLHVPI 257
           AP FFS+ERAAAPYFG+K                                          
Sbjct: 183 APAFFSIERAAAPYFGLKG----------------------------------------- 242

Query: 258 SYLDQRLTQVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLVLPLTFSVS 317
                     Y +H+NGYVE++G+KFLW+ KRS  K T+PG LDHLVAGGL         
Sbjct: 243 ----------YAIHVNGYVERDGQKFLWIGKRSLAKSTYPGKLDHLVAGGL--------- 302

Query: 318 SARFVHANLDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIPVGAISYTD 377
                                 P GI+  ENL+KECEEEAGI + ++  AI VG +SY D
Sbjct: 303 ----------------------PHGISVCENLVKECEEEAGISKVLADRAIAVGVVSYMD 362

Query: 378 IKGYGYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPNCSLVI 437
           I  Y + RDV FCYDL+LP+ F+P NQDGEV+ F L+PV  VANV+R+T  FK +CSLVI
Sbjct: 363 IDRYCFTRDVLFCYDLELPQDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVI 364

Query: 438 INFLFRHGYIKPESLGYLELLQSLRSGTC 455
           I+FLFRHG I+PES GYL+L + LR+G C
Sbjct: 423 IDFLFRHGLIRPESPGYLDLYRRLRNGDC 364

BLAST of Lsi06G009000 vs. ExPASy Swiss-Prot
Match: P47173 (Uncharacterized protein YJR142W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YJR142W PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 9.6e-27
Identity = 79/206 (38.35%), Postives = 111/206 (53.88%), Query Frame = 0

Query: 256 YGVHMNGYV--EKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLVLPLTFSVSSARFVHAN 315
           YG+H+NGYV   K  K   WV +RS+TK T+P MLD+++AGGL                 
Sbjct: 156 YGIHINGYVLDPKSKKVQFWVPRRSKTKQTWPLMLDNIIAGGLGY--------------- 215

Query: 316 LDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAI-PVGAISYT----DIKG 375
                         P GI   E ++KE  EEA + +S+ ++ I   G++SY     DI  
Sbjct: 216 --------------PYGIY--ETVLKESMEEANLEKSVIEDNIKATGSVSYLYFTGDISV 275

Query: 376 YGYKR-------DVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPNC 435
             + +       +VQ+ YDLKL E  IP+  DGEVE F L  +    N +R+ + FKPNC
Sbjct: 276 TKFNKESDFIVGEVQYVYDLKLSEDIIPKPNDGEVESFNLFSLQETINALRKKE-FKPNC 329

Query: 436 SLVIINFLFRHGYIKPES-LGYLELL 447
           +LV+++FL RHGYI PE+   YLEL+
Sbjct: 336 ALVMVDFLIRHGYITPENEPNYLELV 329

BLAST of Lsi06G009000 vs. ExPASy Swiss-Prot
Match: P41888 (Thiamine pyrophosphokinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tnr3 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 7.9e-21
Identity = 66/196 (33.67%), Postives = 98/196 (50.00%), Query Frame = 0

Query: 257 GVHMNGYV--EKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLVLPLTFSVSSARFVHANL 316
           GVH   Y+   KE    +WV +RS TK T+P  LD+ VAGG+                  
Sbjct: 136 GVHCTMYIPATKEHPLRIWVPRRSPTKQTWPNYLDNSVAGGIA----------------- 195

Query: 317 DTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIPVGAISYTDI-KGYGYKR 376
                          G +    ++KE  EEA +  S S   IP G +SY  + K +  + 
Sbjct: 196 --------------HGDSVIGTMIKEFSEEANLDVS-SMNLIPCGTVSYIKMEKRHWIQP 255

Query: 377 DVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPNCSLVIINFLFRHG 436
           ++Q+ +DL + +  IP   DGEV GF LLP+  V + +   + FKPNC+LV+++FL RHG
Sbjct: 256 ELQYVFDLPVDDLVIPRINDGEVAGFSLLPLNQVLHEL-ELKSFKPNCALVLLDFLIRHG 298

Query: 437 YIKPESLGYLELLQSL 450
            I P+   YL+ L+ +
Sbjct: 316 IITPQHPQYLQTLERI 298

BLAST of Lsi06G009000 vs. ExPASy TrEMBL
Match: A0A1S3CJ09 (nudix hydrolase 20, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501537 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 1.1e-182
Identity = 338/455 (74.29%), Postives = 349/455 (76.70%), Query Frame = 0

Query: 1   MACKSLATHSFRNFSTVSSFCNVSTISRTTYPMTSTFRTTIHRSISVASPSVSVTAGSFT 60
           MA KSLA HSFRNFSTV SFCN STI RTTY   STFR T+HRSISVAS  VSVT GSFT
Sbjct: 1   MASKSLA-HSFRNFSTVPSFCNASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFT 60

Query: 61  WDDVVHVSLPKSFQDDPSDLTGYFEKVKLCNLGSDTQSEFLPFVIDDQIVGYVHHGFAKH 120
           WDDVVHVS P+SFQDDP+DLTGYFEKVKLCN GSDTQSEF PFVID QIVGYVHHGFAKH
Sbjct: 61  WDDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKH 120

Query: 121 LKQYANVFAFPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGEEVIPGTRNELYPV 180
           L+QY NVF F +DDSVK GA LTLHESLKTP+DRT AVGDVVKCLGEEVIPGTRNEL+PV
Sbjct: 121 LQQYPNVFTFSQDDSVKVGACLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPV 180

Query: 181 TSSFGAPNFFSLERAAAPYFGIKALENSRPFVLLNLFEVTEDFANNFMVLKEFYLATAFL 240
           TSSFGAPNFFSLERAAAPYFGIK                                     
Sbjct: 181 TSSFGAPNFFSLERAAAPYFGIK------------------------------------- 240

Query: 241 LHVPISYLDQRLTQVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLVLPL 300
                         VYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGL    
Sbjct: 241 --------------VYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGL---- 300

Query: 301 TFSVSSARFVHANLDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIPVGA 360
                                      PQGI+CGENLMKEC+EEAGIPRS+SKEAIPVGA
Sbjct: 301 ---------------------------PQGISCGENLMKECKEEAGIPRSVSKEAIPVGA 360

Query: 361 ISYTDIKGYGYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPN 420
           ISYTDIKGYGYKRDVQFCYDLKLPESF+PENQDGEVEGFMLLPVTNVANVIRRTQ FKPN
Sbjct: 361 ISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPN 372

Query: 421 CSLVIINFLFRHGYIKPESLGYLELLQSLRSGTCF 456
           CSLVIINFLFRHGYIKPES GYLELLQSLRSGTCF
Sbjct: 421 CSLVIINFLFRHGYIKPESSGYLELLQSLRSGTCF 372

BLAST of Lsi06G009000 vs. ExPASy TrEMBL
Match: A0A5A7TT66 (Nudix hydrolase 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G001740 PE=4 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 9.5e-179
Identity = 338/478 (70.71%), Postives = 349/478 (73.01%), Query Frame = 0

Query: 1   MACKSLATHSFRNFSTVSSFCNVSTISRTTYPMTSTFRTTIHRSISVASPSVSVTAGSFT 60
           MA KSLA HSFRNFSTV SFCN STI RTTY   STFR T+HRSISVAS  VSVT GSFT
Sbjct: 1   MASKSLA-HSFRNFSTVPSFCNASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFT 60

Query: 61  WDDVVHVSLPKSFQDDPSDLTGYFEKVKLCNLG-----------------------SDTQ 120
           WDDVVHVS P+SFQDDP+DLTGYFEKVKLCN G                       SDTQ
Sbjct: 61  WDDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSVGLFLLFFSCFAVQTKDVKAFLSDTQ 120

Query: 121 SEFLPFVIDDQIVGYVHHGFAKHLKQYANVFAFPRDDSVKFGAYLTLHESLKTPKDRTLA 180
           SEF PFVID QIVGYVHHGFAKHL+QY NVF F +DDSVK GA LTLHESLKTP+DRT A
Sbjct: 121 SEFFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVKVGACLTLHESLKTPEDRTRA 180

Query: 181 VGDVVKCLGEEVIPGTRNELYPVTSSFGAPNFFSLERAAAPYFGIKALENSRPFVLLNLF 240
           VGDVVKCLGEEVIPGTRNEL+PVTSSFGAPNFFSLERAAAPYFGIK              
Sbjct: 181 VGDVVKCLGEEVIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGIK-------------- 240

Query: 241 EVTEDFANNFMVLKEFYLATAFLLHVPISYLDQRLTQVYGVHMNGYVEKEGKKFLWVAKR 300
                                                VYGVHMNGYVEKEGKKFLWVAKR
Sbjct: 241 -------------------------------------VYGVHMNGYVEKEGKKFLWVAKR 300

Query: 301 SQTKPTFPGMLDHLVAGGLVLPLTFSVSSARFVHANLDTNQQKKRQGKCKPQGIACGENL 360
           SQTKPTFPGMLDHLVAGGL                               PQGI+CGENL
Sbjct: 301 SQTKPTFPGMLDHLVAGGL-------------------------------PQGISCGENL 360

Query: 361 MKECEEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKLPESFIPENQDGEVE 420
           MKEC+EEAGIPRS+SKEAIPVGAISYTDIKGYGYKRDVQFCYDLKLPESF+PENQDGEVE
Sbjct: 361 MKECKEEAGIPRSVSKEAIPVGAISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVE 395

Query: 421 GFMLLPVTNVANVIRRTQLFKPNCSLVIINFLFRHGYIKPESLGYLELLQSLRSGTCF 456
           GFMLLPVTNVANVIRRTQ FKPNCSLVIINFLFRHGYIKPES GYLELLQSLRSGTCF
Sbjct: 421 GFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYLELLQSLRSGTCF 395

BLAST of Lsi06G009000 vs. ExPASy TrEMBL
Match: A0A0A0LWL2 (Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G386580 PE=4 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 1.2e-178
Identity = 334/455 (73.41%), Postives = 346/455 (76.04%), Query Frame = 0

Query: 1   MACKSLATHSFRNFSTVSSFCNVSTISRTTYPMTSTFRTTIHRSISVASPSVSVTAGSFT 60
           MA KSLA HS RNFSTVSSFCN STISRTTY   STFR  +HRSISVAS  VSVTAGS T
Sbjct: 1   MASKSLA-HSLRNFSTVSSFCNASTISRTTYTTISTFRPKVHRSISVASRPVSVTAGSLT 60

Query: 61  WDDVVHVSLPKSFQDDPSDLTGYFEKVKLCNLGSDTQSEFLPFVIDDQIVGYVHHGFAKH 120
           WDDVVHVSLP+SFQDDP+DLTGYFEKVKLCN GSDTQSEF PFVID QIVGY+HHGF KH
Sbjct: 61  WDDVVHVSLPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYMHHGFVKH 120

Query: 121 LKQYANVFAFPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGEEVIPGTRNELYPV 180
           L+QY  VF   RDDSVKFGA+LTLHESLKTP+DRT AVGDVVKCLGEEVIPGTRNEL+PV
Sbjct: 121 LQQYPKVFT--RDDSVKFGAFLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPV 180

Query: 181 TSSFGAPNFFSLERAAAPYFGIKALENSRPFVLLNLFEVTEDFANNFMVLKEFYLATAFL 240
           TSSFGA +FFSLERAAAPYFGIK                                     
Sbjct: 181 TSSFGALSFFSLERAAAPYFGIK------------------------------------- 240

Query: 241 LHVPISYLDQRLTQVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLVLPL 300
                         VYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGL    
Sbjct: 241 --------------VYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGL---- 300

Query: 301 TFSVSSARFVHANLDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIPVGA 360
                                      PQGI CGENLMKEC+EEAGIPRSISKEAIPVGA
Sbjct: 301 ---------------------------PQGIPCGENLMKECQEEAGIPRSISKEAIPVGA 360

Query: 361 ISYTDIKGYGYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPN 420
           ISYTDIKGYGYKRDVQFCYDLKLPESF+PENQDGEVEGFMLLPVTNVANVIRRTQ FKPN
Sbjct: 361 ISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPN 370

Query: 421 CSLVIINFLFRHGYIKPESLGYLELLQSLRSGTCF 456
           CSLVII+FLFRHGYIKPES GYLELLQSLR GTCF
Sbjct: 421 CSLVIIDFLFRHGYIKPESSGYLELLQSLRGGTCF 370

BLAST of Lsi06G009000 vs. ExPASy TrEMBL
Match: A0A6J1CSY3 (nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111014470 PE=4 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 1.2e-176
Identity = 332/458 (72.49%), Postives = 349/458 (76.20%), Query Frame = 0

Query: 1   MACKSLATHS--FRNFSTVSSFCNVSTISRTTYPMTSTFR-TTIHRSISVASPSVSVTAG 60
           MACKSLA+HS  FRNFST+SS CN STISRTTYPM STFR T +HRSISVAS SVSV A 
Sbjct: 17  MACKSLASHSHRFRNFSTISSNCNASTISRTTYPMASTFRPTVVHRSISVASRSVSV-AS 76

Query: 61  SFTWDDVVHVSLPKSFQDDPSDLTGYFEKVKLCNLGSDTQSEFLPFVIDDQIVGYVHHGF 120
           SFTWDDV+ +SLP+ +QDDPSDLTGYFEKVKLCN GSD QSEFLPFV+DDQIVGYVHHGF
Sbjct: 77  SFTWDDVLRISLPEIYQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVVDDQIVGYVHHGF 136

Query: 121 AKHLKQYANVFAFPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGEEVIPGTRNEL 180
           A+HLKQYANVF F  DDSV+F AYLTLHESLKTP+DRT AVGDVVKCLGEE+IPGTRNEL
Sbjct: 137 AEHLKQYANVFTFSGDDSVQFRAYLTLHESLKTPEDRTQAVGDVVKCLGEEIIPGTRNEL 196

Query: 181 YPVTSSFGAPNFFSLERAAAPYFGIKALENSRPFVLLNLFEVTEDFANNFMVLKEFYLAT 240
           YPVTSSFGAPNFFSLERAAAPYFGIK                                  
Sbjct: 197 YPVTSSFGAPNFFSLERAAAPYFGIK---------------------------------- 256

Query: 241 AFLLHVPISYLDQRLTQVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLV 300
                            VYGVHMNGYVEKEGKKFLWVAKRSQTK TFPGMLDHLVAGGL 
Sbjct: 257 -----------------VYGVHMNGYVEKEGKKFLWVAKRSQTKQTFPGMLDHLVAGGL- 316

Query: 301 LPLTFSVSSARFVHANLDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIP 360
                                         PQGI C ENLMKECEEEA IPRS+S EAIP
Sbjct: 317 ------------------------------PQGIPCVENLMKECEEEACIPRSLSNEAIP 376

Query: 361 VGAISYTDIKGYGYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLF 420
           VGA+SY+DIKG+ YKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLF
Sbjct: 377 VGAVSYSDIKGHTYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLF 391

Query: 421 KPNCSLVIINFLFRHGYIKPESLGYLELLQSLRSGTCF 456
           KPNCSLVII+FLFRHGYI PES GYLELLQSLRSGTCF
Sbjct: 437 KPNCSLVIIDFLFRHGYIYPESSGYLELLQSLRSGTCF 391

BLAST of Lsi06G009000 vs. ExPASy TrEMBL
Match: A0A6J1JI14 (LOW QUALITY PROTEIN: nudix hydrolase 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111485288 PE=4 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.4e-174
Identity = 326/455 (71.65%), Postives = 338/455 (74.29%), Query Frame = 0

Query: 1   MACKSLATHSFRNFSTVSSFCNVSTISRTTYPMTSTFRTTIHRSISVASPSVSVTAGSFT 60
           MACKSL  H F NFS VSS  N S ISRTTYPM STFR  + RSISVA       AG+F+
Sbjct: 17  MACKSLVAHRFHNFSIVSSLYNASKISRTTYPMLSTFRPAVTRSISVA-------AGNFS 76

Query: 61  WDDVVHVSLPKSFQDDPSDLTGYFEKVKLCNLGSDTQSEFLPFVIDDQIVGYVHHGFAKH 120
           WDDVVH+SLP SFQDDPSDLTGYFEKVKLCN GSDTQSEFLPFVIDD IVGYVHHGFAKH
Sbjct: 77  WDDVVHISLPSSFQDDPSDLTGYFEKVKLCNRGSDTQSEFLPFVIDDLIVGYVHHGFAKH 136

Query: 121 LKQYANVFAFPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGEEVIPGTRNELYPV 180
           LKQY NVF FPRDD  +FGAYLTLHESLKTP+DRT AVGDVVKCLG+EVIPGTRNELYPV
Sbjct: 137 LKQYPNVFIFPRDDCGQFGAYLTLHESLKTPEDRTHAVGDVVKCLGKEVIPGTRNELYPV 196

Query: 181 TSSFGAPNFFSLERAAAPYFGIKALENSRPFVLLNLFEVTEDFANNFMVLKEFYLATAFL 240
           TSSFGA NFFSLERAAAPYFGIK                                     
Sbjct: 197 TSSFGASNFFSLERAAAPYFGIK------------------------------------- 256

Query: 241 LHVPISYLDQRLTQVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLVLPL 300
                         VYGVHMNGYVEKEG+KFLWVAKRSQTKPTFPGMLDHLVAGGL    
Sbjct: 257 --------------VYGVHMNGYVEKEGEKFLWVAKRSQTKPTFPGMLDHLVAGGL---- 316

Query: 301 TFSVSSARFVHANLDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIPVGA 360
                                      PQGI CGEN+MKECEEEAGIPRSISK AIPVGA
Sbjct: 317 ---------------------------PQGIPCGENVMKECEEEAGIPRSISKGAIPVGA 376

Query: 361 ISYTDIKGYGYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPN 420
           ISYTDIKG+ YKRDVQFCYDLKLPESFIPENQDGEVEGFMLL VTNVANVIRRTQLFKPN
Sbjct: 377 ISYTDIKGFSYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLLVTNVANVIRRTQLFKPN 382

Query: 421 CSLVIINFLFRHGYIKPESLGYLELLQSLRSGTCF 456
           C+LVII+FLFRHGYI PES GYLELLQSLRSGTCF
Sbjct: 437 CTLVIIDFLFRHGYINPESSGYLELLQSLRSGTCF 382

BLAST of Lsi06G009000 vs. NCBI nr
Match: XP_038879265.1 (nudix hydrolase 20, chloroplastic-like isoform X3 [Benincasa hispida])

HSP 1 Score: 679.9 bits (1753), Expect = 1.6e-191
Identity = 352/455 (77.36%), Postives = 358/455 (78.68%), Query Frame = 0

Query: 1   MACKSLATHSFRNFSTVSSFCNVSTISRTTYPMTSTFRTTIHRSISVASPSVSVTAGSFT 60
           MA KS+ATHSFRNFSTVSSFCN STISRTTYPMTSTFR T+  SISVAS SVSV AGSFT
Sbjct: 1   MAYKSMATHSFRNFSTVSSFCNASTISRTTYPMTSTFRPTLLHSISVASRSVSVAAGSFT 60

Query: 61  WDDVVHVSLPKSFQDDPSDLTGYFEKVKLCNLGSDTQSEFLPFVIDDQIVGYVHHGFAKH 120
           WDDVVHVSLP+SFQDDPSDLTGYFEKVKLCN GSDTQSE LPFVIDDQIVGYVHHGFAKH
Sbjct: 61  WDDVVHVSLPESFQDDPSDLTGYFEKVKLCNRGSDTQSEILPFVIDDQIVGYVHHGFAKH 120

Query: 121 LKQYANVFAFPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGEEVIPGTRNELYPV 180
           LKQY NVF FPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGE+VIPGTRNELYPV
Sbjct: 121 LKQYPNVFTFPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGEKVIPGTRNELYPV 180

Query: 181 TSSFGAPNFFSLERAAAPYFGIKALENSRPFVLLNLFEVTEDFANNFMVLKEFYLATAFL 240
           TSSFGAPNFFSLERAAAPYFGIK                                     
Sbjct: 181 TSSFGAPNFFSLERAAAPYFGIK------------------------------------- 240

Query: 241 LHVPISYLDQRLTQVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLVLPL 300
                         VYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGL    
Sbjct: 241 --------------VYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGL---- 300

Query: 301 TFSVSSARFVHANLDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIPVGA 360
                                      PQGI CGENLMKECEEEAGIPRSISKEAIPVGA
Sbjct: 301 ---------------------------PQGIPCGENLMKECEEEAGIPRSISKEAIPVGA 360

Query: 361 ISYTDIKGYGYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPN 420
           ISYTDIKGYGYKRDVQFCYDLKLPE FIPENQDGEVEGFMLLPV NVANVIRRTQLFKPN
Sbjct: 361 ISYTDIKGYGYKRDVQFCYDLKLPEDFIPENQDGEVEGFMLLPVANVANVIRRTQLFKPN 373

Query: 421 CSLVIINFLFRHGYIKPESLGYLELLQSLRSGTCF 456
           CSLVI++FLFRHGYIKPES GYLELLQSLRSGTCF
Sbjct: 421 CSLVIMDFLFRHGYIKPESSGYLELLQSLRSGTCF 373

BLAST of Lsi06G009000 vs. NCBI nr
Match: XP_008463362.1 (PREDICTED: nudix hydrolase 20, chloroplastic-like isoform X1 [Cucumis melo])

HSP 1 Score: 649.4 bits (1674), Expect = 2.2e-182
Identity = 338/455 (74.29%), Postives = 349/455 (76.70%), Query Frame = 0

Query: 1   MACKSLATHSFRNFSTVSSFCNVSTISRTTYPMTSTFRTTIHRSISVASPSVSVTAGSFT 60
           MA KSLA HSFRNFSTV SFCN STI RTTY   STFR T+HRSISVAS  VSVT GSFT
Sbjct: 1   MASKSLA-HSFRNFSTVPSFCNASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFT 60

Query: 61  WDDVVHVSLPKSFQDDPSDLTGYFEKVKLCNLGSDTQSEFLPFVIDDQIVGYVHHGFAKH 120
           WDDVVHVS P+SFQDDP+DLTGYFEKVKLCN GSDTQSEF PFVID QIVGYVHHGFAKH
Sbjct: 61  WDDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKH 120

Query: 121 LKQYANVFAFPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGEEVIPGTRNELYPV 180
           L+QY NVF F +DDSVK GA LTLHESLKTP+DRT AVGDVVKCLGEEVIPGTRNEL+PV
Sbjct: 121 LQQYPNVFTFSQDDSVKVGACLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPV 180

Query: 181 TSSFGAPNFFSLERAAAPYFGIKALENSRPFVLLNLFEVTEDFANNFMVLKEFYLATAFL 240
           TSSFGAPNFFSLERAAAPYFGIK                                     
Sbjct: 181 TSSFGAPNFFSLERAAAPYFGIK------------------------------------- 240

Query: 241 LHVPISYLDQRLTQVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLVLPL 300
                         VYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGL    
Sbjct: 241 --------------VYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGL---- 300

Query: 301 TFSVSSARFVHANLDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIPVGA 360
                                      PQGI+CGENLMKEC+EEAGIPRS+SKEAIPVGA
Sbjct: 301 ---------------------------PQGISCGENLMKECKEEAGIPRSVSKEAIPVGA 360

Query: 361 ISYTDIKGYGYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPN 420
           ISYTDIKGYGYKRDVQFCYDLKLPESF+PENQDGEVEGFMLLPVTNVANVIRRTQ FKPN
Sbjct: 361 ISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPN 372

Query: 421 CSLVIINFLFRHGYIKPESLGYLELLQSLRSGTCF 456
           CSLVIINFLFRHGYIKPES GYLELLQSLRSGTCF
Sbjct: 421 CSLVIINFLFRHGYIKPESSGYLELLQSLRSGTCF 372

BLAST of Lsi06G009000 vs. NCBI nr
Match: KAA0046622.1 (nudix hydrolase 20 [Cucumis melo var. makuwa])

HSP 1 Score: 636.3 bits (1640), Expect = 2.0e-178
Identity = 338/478 (70.71%), Postives = 349/478 (73.01%), Query Frame = 0

Query: 1   MACKSLATHSFRNFSTVSSFCNVSTISRTTYPMTSTFRTTIHRSISVASPSVSVTAGSFT 60
           MA KSLA HSFRNFSTV SFCN STI RTTY   STFR T+HRSISVAS  VSVT GSFT
Sbjct: 1   MASKSLA-HSFRNFSTVPSFCNASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFT 60

Query: 61  WDDVVHVSLPKSFQDDPSDLTGYFEKVKLCNLG-----------------------SDTQ 120
           WDDVVHVS P+SFQDDP+DLTGYFEKVKLCN G                       SDTQ
Sbjct: 61  WDDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSVGLFLLFFSCFAVQTKDVKAFLSDTQ 120

Query: 121 SEFLPFVIDDQIVGYVHHGFAKHLKQYANVFAFPRDDSVKFGAYLTLHESLKTPKDRTLA 180
           SEF PFVID QIVGYVHHGFAKHL+QY NVF F +DDSVK GA LTLHESLKTP+DRT A
Sbjct: 121 SEFFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVKVGACLTLHESLKTPEDRTRA 180

Query: 181 VGDVVKCLGEEVIPGTRNELYPVTSSFGAPNFFSLERAAAPYFGIKALENSRPFVLLNLF 240
           VGDVVKCLGEEVIPGTRNEL+PVTSSFGAPNFFSLERAAAPYFGIK              
Sbjct: 181 VGDVVKCLGEEVIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGIK-------------- 240

Query: 241 EVTEDFANNFMVLKEFYLATAFLLHVPISYLDQRLTQVYGVHMNGYVEKEGKKFLWVAKR 300
                                                VYGVHMNGYVEKEGKKFLWVAKR
Sbjct: 241 -------------------------------------VYGVHMNGYVEKEGKKFLWVAKR 300

Query: 301 SQTKPTFPGMLDHLVAGGLVLPLTFSVSSARFVHANLDTNQQKKRQGKCKPQGIACGENL 360
           SQTKPTFPGMLDHLVAGGL                               PQGI+CGENL
Sbjct: 301 SQTKPTFPGMLDHLVAGGL-------------------------------PQGISCGENL 360

Query: 361 MKECEEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKLPESFIPENQDGEVE 420
           MKEC+EEAGIPRS+SKEAIPVGAISYTDIKGYGYKRDVQFCYDLKLPESF+PENQDGEVE
Sbjct: 361 MKECKEEAGIPRSVSKEAIPVGAISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVE 395

Query: 421 GFMLLPVTNVANVIRRTQLFKPNCSLVIINFLFRHGYIKPESLGYLELLQSLRSGTCF 456
           GFMLLPVTNVANVIRRTQ FKPNCSLVIINFLFRHGYIKPES GYLELLQSLRSGTCF
Sbjct: 421 GFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYLELLQSLRSGTCF 395

BLAST of Lsi06G009000 vs. NCBI nr
Match: XP_004148712.1 (nudix hydrolase 20, chloroplastic isoform X3 [Cucumis sativus] >KGN65374.1 hypothetical protein Csa_020063 [Cucumis sativus])

HSP 1 Score: 636.0 bits (1639), Expect = 2.6e-178
Identity = 334/455 (73.41%), Postives = 346/455 (76.04%), Query Frame = 0

Query: 1   MACKSLATHSFRNFSTVSSFCNVSTISRTTYPMTSTFRTTIHRSISVASPSVSVTAGSFT 60
           MA KSLA HS RNFSTVSSFCN STISRTTY   STFR  +HRSISVAS  VSVTAGS T
Sbjct: 1   MASKSLA-HSLRNFSTVSSFCNASTISRTTYTTISTFRPKVHRSISVASRPVSVTAGSLT 60

Query: 61  WDDVVHVSLPKSFQDDPSDLTGYFEKVKLCNLGSDTQSEFLPFVIDDQIVGYVHHGFAKH 120
           WDDVVHVSLP+SFQDDP+DLTGYFEKVKLCN GSDTQSEF PFVID QIVGY+HHGF KH
Sbjct: 61  WDDVVHVSLPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYMHHGFVKH 120

Query: 121 LKQYANVFAFPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGEEVIPGTRNELYPV 180
           L+QY  VF   RDDSVKFGA+LTLHESLKTP+DRT AVGDVVKCLGEEVIPGTRNEL+PV
Sbjct: 121 LQQYPKVFT--RDDSVKFGAFLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPV 180

Query: 181 TSSFGAPNFFSLERAAAPYFGIKALENSRPFVLLNLFEVTEDFANNFMVLKEFYLATAFL 240
           TSSFGA +FFSLERAAAPYFGIK                                     
Sbjct: 181 TSSFGALSFFSLERAAAPYFGIK------------------------------------- 240

Query: 241 LHVPISYLDQRLTQVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLVLPL 300
                         VYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGL    
Sbjct: 241 --------------VYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGL---- 300

Query: 301 TFSVSSARFVHANLDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIPVGA 360
                                      PQGI CGENLMKEC+EEAGIPRSISKEAIPVGA
Sbjct: 301 ---------------------------PQGIPCGENLMKECQEEAGIPRSISKEAIPVGA 360

Query: 361 ISYTDIKGYGYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPN 420
           ISYTDIKGYGYKRDVQFCYDLKLPESF+PENQDGEVEGFMLLPVTNVANVIRRTQ FKPN
Sbjct: 361 ISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPN 370

Query: 421 CSLVIINFLFRHGYIKPESLGYLELLQSLRSGTCF 456
           CSLVII+FLFRHGYIKPES GYLELLQSLR GTCF
Sbjct: 421 CSLVIIDFLFRHGYIKPESSGYLELLQSLRGGTCF 370

BLAST of Lsi06G009000 vs. NCBI nr
Match: XP_022144905.1 (nudix hydrolase 20, chloroplastic-like [Momordica charantia])

HSP 1 Score: 629.4 bits (1622), Expect = 2.4e-176
Identity = 332/458 (72.49%), Postives = 349/458 (76.20%), Query Frame = 0

Query: 1   MACKSLATHS--FRNFSTVSSFCNVSTISRTTYPMTSTFR-TTIHRSISVASPSVSVTAG 60
           MACKSLA+HS  FRNFST+SS CN STISRTTYPM STFR T +HRSISVAS SVSV A 
Sbjct: 17  MACKSLASHSHRFRNFSTISSNCNASTISRTTYPMASTFRPTVVHRSISVASRSVSV-AS 76

Query: 61  SFTWDDVVHVSLPKSFQDDPSDLTGYFEKVKLCNLGSDTQSEFLPFVIDDQIVGYVHHGF 120
           SFTWDDV+ +SLP+ +QDDPSDLTGYFEKVKLCN GSD QSEFLPFV+DDQIVGYVHHGF
Sbjct: 77  SFTWDDVLRISLPEIYQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVVDDQIVGYVHHGF 136

Query: 121 AKHLKQYANVFAFPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGEEVIPGTRNEL 180
           A+HLKQYANVF F  DDSV+F AYLTLHESLKTP+DRT AVGDVVKCLGEE+IPGTRNEL
Sbjct: 137 AEHLKQYANVFTFSGDDSVQFRAYLTLHESLKTPEDRTQAVGDVVKCLGEEIIPGTRNEL 196

Query: 181 YPVTSSFGAPNFFSLERAAAPYFGIKALENSRPFVLLNLFEVTEDFANNFMVLKEFYLAT 240
           YPVTSSFGAPNFFSLERAAAPYFGIK                                  
Sbjct: 197 YPVTSSFGAPNFFSLERAAAPYFGIK---------------------------------- 256

Query: 241 AFLLHVPISYLDQRLTQVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLV 300
                            VYGVHMNGYVEKEGKKFLWVAKRSQTK TFPGMLDHLVAGGL 
Sbjct: 257 -----------------VYGVHMNGYVEKEGKKFLWVAKRSQTKQTFPGMLDHLVAGGL- 316

Query: 301 LPLTFSVSSARFVHANLDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIP 360
                                         PQGI C ENLMKECEEEA IPRS+S EAIP
Sbjct: 317 ------------------------------PQGIPCVENLMKECEEEACIPRSLSNEAIP 376

Query: 361 VGAISYTDIKGYGYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLF 420
           VGA+SY+DIKG+ YKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLF
Sbjct: 377 VGAVSYSDIKGHTYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLF 391

Query: 421 KPNCSLVIINFLFRHGYIKPESLGYLELLQSLRSGTCF 456
           KPNCSLVII+FLFRHGYI PES GYLELLQSLRSGTCF
Sbjct: 437 KPNCSLVIIDFLFRHGYIYPESSGYLELLQSLRSGTCF 391

BLAST of Lsi06G009000 vs. TAIR 10
Match: AT5G19460.1 (nudix hydrolase homolog 20 )

HSP 1 Score: 372.9 bits (956), Expect = 3.8e-103
Identity = 202/448 (45.09%), Postives = 268/448 (59.82%), Query Frame = 0

Query: 15  STVSSFCNVSTISRTTYPM-----TSTFRTTIHRSISVASPSVSVTAGSFTWDDVVHVSL 74
           +  +S  +   I+R   P+     +S   + +  S ++++ +    + SFTWDDV+    
Sbjct: 11  TVTTSLFSSHAITRRVLPILRWRSSSMSLSPLRHSRALSAATTVPISSSFTWDDVIETG- 70

Query: 75  PKSFQDDPSDLTGYFEKVKLCNLGSDTQSEFLPFVIDDQIVGYVHHGFAKHLKQYANVFA 134
               + + SDLTG+FEK+  CN GS+   EF+PFVI++QIVGY+H  F ++L+++ ++F 
Sbjct: 71  --RAEYNSSDLTGFFEKINRCNRGSEKLGEFIPFVIEEQIVGYIHKRFTEYLREFHDIFT 130

Query: 135 FPRDDSV--KFGAYLTLHESLKTPKDRTLAVGDVVKCLGEE-VIPGTRNELYPVTSSFGA 194
           F ++ S   +   Y+TL+  L+ P+DRT AV DV+K LG++ +IPG RNELYPV  SF A
Sbjct: 131 FSQNGSCPDRVDGYVTLNLMLQKPEDRTRAVADVIKILGDKGIIPGIRNELYPVKPSFNA 190

Query: 195 PNFFSLERAAAPYFGIKALENSRPFVLLNLFEVTEDFANNFMVLKEFYLATAFLLHVPIS 254
           P FFSLERAAAPYFGIK                                           
Sbjct: 191 PVFFSLERAAAPYFGIKG------------------------------------------ 250

Query: 255 YLDQRLTQVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLVLPLTFSVSS 314
                    YGVHMNGYVE++G+K LW+ KRS +K T+PGMLDHLVAGGL          
Sbjct: 251 ---------YGVHMNGYVERDGQKLLWIGKRSLSKSTYPGMLDHLVAGGL---------- 310

Query: 315 ARFVHANLDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIPVGAISYTDI 374
                                P GI+CG NL+KECEEEAGI R+I+  AI VGA+SY DI
Sbjct: 311 ---------------------PHGISCGGNLVKECEEEAGISRAIADRAIAVGAVSYLDI 370

Query: 375 KGYGYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPNCSLVII 434
             Y +KRDV FCYDL+LPE F+P+NQDGEVE F L+PV  VA+VI++T  FK NCSLVII
Sbjct: 371 DQYCFKRDVLFCYDLELPEDFVPKNQDGEVESFKLIPVAQVASVIKKTSFFKANCSLVII 373

Query: 435 NFLFRHGYIKPESLGYLELLQSLRSGTC 455
           +FLFRHG+I+PES GYL+L Q LR+  C
Sbjct: 431 DFLFRHGFIRPESSGYLDLYQRLRNRDC 373

BLAST of Lsi06G009000 vs. TAIR 10
Match: AT5G19470.1 (nudix hydrolase homolog 24 )

HSP 1 Score: 347.1 bits (889), Expect = 2.2e-95
Identity = 191/449 (42.54%), Postives = 255/449 (56.79%), Query Frame = 0

Query: 18  SSFCNVSTISRTTYPMTSTFRTTIHRSISVA-SPSVSVT----------AGSFTWDDVVH 77
           S+FC++     + +   +   T   RS S + SP + ++          + SFTW+DV  
Sbjct: 3   SAFCSLCPTPTSLFSSHALIPTLQWRSSSSSRSPPLHISRVLSVETVPLSPSFTWNDVFE 62

Query: 78  VSLPKSFQDDPSDLTGYFEKVKLCNLGSDTQSEFLPFVIDDQIVGYVHHGFAKHLKQYAN 137
            S  +    + SDLTG+ EKV  CN G +   EF+PFVI++QIVGY+H GF K+L+ + +
Sbjct: 63  NSRKEYVPQNSSDLTGFLEKVDRCNRGLEKLGEFIPFVIEEQIVGYIHKGFTKYLRDFND 122

Query: 138 VFAFPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGEE-VIPGTRNELYPVTSSFG 197
           +F F      ++G ++TL+  L  P++RT AV  V+K LG + +IPG RNELYPV  SF 
Sbjct: 123 IFTFS-----QYGGHVTLNMMLDKPEERTRAVAHVIKILGNKGIIPGIRNELYPVKPSFN 182

Query: 198 APNFFSLERAAAPYFGIKALENSRPFVLLNLFEVTEDFANNFMVLKEFYLATAFLLHVPI 257
           AP FFS+ERAAAPYFG+K                                          
Sbjct: 183 APAFFSIERAAAPYFGLKG----------------------------------------- 242

Query: 258 SYLDQRLTQVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLVLPLTFSVS 317
                     Y +H+NGYVE++G+KFLW+ KRS  K T+PG LDHLVAGGL         
Sbjct: 243 ----------YAIHVNGYVERDGQKFLWIGKRSLAKSTYPGKLDHLVAGGL--------- 302

Query: 318 SARFVHANLDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIPVGAISYTD 377
                                 P GI+  ENL+KECEEEAGI + ++  AI VG +SY D
Sbjct: 303 ----------------------PHGISVCENLVKECEEEAGISKVLADRAIAVGVVSYMD 362

Query: 378 IKGYGYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPNCSLVI 437
           I  Y + RDV FCYDL+LP+ F+P NQDGEV+ F L+PV  VANV+R+T  FK +CSLVI
Sbjct: 363 IDRYCFTRDVLFCYDLELPQDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVI 364

Query: 438 INFLFRHGYIKPESLGYLELLQSLRSGTC 455
           I+FLFRHG I+PES GYL+L + LR+G C
Sbjct: 423 IDFLFRHGLIRPESPGYLDLYRRLRNGDC 364

BLAST of Lsi06G009000 vs. TAIR 10
Match: AT5G19470.2 (nudix hydrolase homolog 24 )

HSP 1 Score: 336.7 bits (862), Expect = 3.0e-92
Identity = 187/449 (41.65%), Postives = 250/449 (55.68%), Query Frame = 0

Query: 18  SSFCNVSTISRTTYPMTSTFRTTIHRSISVA-SPSVSVT----------AGSFTWDDVVH 77
           S+FC++     + +   +   T   RS S + SP + ++          + SFTW+DV  
Sbjct: 3   SAFCSLCPTPTSLFSSHALIPTLQWRSSSSSRSPPLHISRVLSVETVPLSPSFTWNDVFE 62

Query: 78  VSLPKSFQDDPSDLTGYFEKVKLCNLGSDTQSEFLPFVIDDQIVGYVHHGFAKHLKQYAN 137
            S  +    + SDLTG+ EKV  CN G +   EF+PFVI++QIVGY+H GF K+L+ + +
Sbjct: 63  NSRKEYVPQNSSDLTGFLEKVDRCNRGLEKLGEFIPFVIEEQIVGYIHKGFTKYLRDFND 122

Query: 138 VFAFPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGEE-VIPGTRNELYPVTSSFG 197
           +F F      ++G ++TL+  L  P++RT AV  V+K LG + +IPG RNELYPV  SF 
Sbjct: 123 IFTFS-----QYGGHVTLNMMLDKPEERTRAVAHVIKILGNKGIIPGIRNELYPVKPSFN 182

Query: 198 APNFFSLERAAAPYFGIKALENSRPFVLLNLFEVTEDFANNFMVLKEFYLATAFLLHVPI 257
           AP FFS+ERAAAPYFG+K                                          
Sbjct: 183 APAFFSIERAAAPYFGLKG----------------------------------------- 242

Query: 258 SYLDQRLTQVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLVLPLTFSVS 317
                     Y +H+NGYVE++G+KFLW+ KRS  K T+PG LDHLVAGGL         
Sbjct: 243 ----------YAIHVNGYVERDGQKFLWIGKRSLAKSTYPGKLDHLVAGGL--------- 302

Query: 318 SARFVHANLDTNQQKKRQGKCKPQGIACGENLMKECEEEAGIPRSISKEAIPVGAISYTD 377
                                         NL+KECEEEAGI + ++  AI VG +SY D
Sbjct: 303 ------------------------------NLVKECEEEAGISKVLADRAIAVGVVSYMD 356

Query: 378 IKGYGYKRDVQFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPNCSLVI 437
           I  Y + RDV FCYDL+LP+ F+P NQDGEV+ F L+PV  VANV+R+T  FK +CSLVI
Sbjct: 363 IDRYCFTRDVLFCYDLELPQDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVI 356

Query: 438 INFLFRHGYIKPESLGYLELLQSLRSGTC 455
           I+FLFRHG I+PES GYL+L + LR+G C
Sbjct: 423 IDFLFRHGLIRPESPGYLDLYRRLRNGDC 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VXZ05.3e-10245.09Nudix hydrolase 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT20 PE=2... [more]
P0C0263.1e-9442.54Nudix hydrolase 24, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT24 PE=2... [more]
P471739.6e-2738.35Uncharacterized protein YJR142W OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
P418887.9e-2133.67Thiamine pyrophosphokinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843... [more]
Match NameE-valueIdentityDescription
A0A1S3CJ091.1e-18274.29nudix hydrolase 20, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A5A7TT669.5e-17970.71Nudix hydrolase 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G0... [more]
A0A0A0LWL21.2e-17873.41Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G38... [more]
A0A6J1CSY31.2e-17672.49nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1JI141.4e-17471.65LOW QUALITY PROTEIN: nudix hydrolase 20, chloroplastic-like OS=Cucurbita maxima ... [more]
Match NameE-valueIdentityDescription
XP_038879265.11.6e-19177.36nudix hydrolase 20, chloroplastic-like isoform X3 [Benincasa hispida][more]
XP_008463362.12.2e-18274.29PREDICTED: nudix hydrolase 20, chloroplastic-like isoform X1 [Cucumis melo][more]
KAA0046622.12.0e-17870.71nudix hydrolase 20 [Cucumis melo var. makuwa][more]
XP_004148712.12.6e-17873.41nudix hydrolase 20, chloroplastic isoform X3 [Cucumis sativus] >KGN65374.1 hypot... [more]
XP_022144905.12.4e-17672.49nudix hydrolase 20, chloroplastic-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G19460.13.8e-10345.09nudix hydrolase homolog 20 [more]
AT5G19470.12.2e-9542.54nudix hydrolase homolog 24 [more]
AT5G19470.23.0e-9241.65nudix hydrolase homolog 24 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR031804Domain of unknown function DUF4743PFAMPF15916DUF4743coord: 84..204
e-value: 2.8E-34
score: 117.7
NoneNo IPR availableGENE3D3.90.79.10Nucleoside Triphosphate Pyrophosphohydrolasecoord: 253..451
e-value: 4.2E-59
score: 201.4
NoneNo IPR availableGENE3D3.30.750.160coord: 95..205
e-value: 3.4E-28
score: 100.0
NoneNo IPR availablePANTHERPTHR13622THIAMIN PYROPHOSPHOKINASEcoord: 254..452
coord: 59..204
NoneNo IPR availablePANTHERPTHR13622:SF10SI:DKEY-6N6.2coord: 254..452
coord: 59..204
IPR000086NUDIX hydrolase domainPROSITEPS51462NUDIXcoord: 255..427
score: 10.00755
IPR015797NUDIX hydrolase-like domain superfamilySUPERFAMILY55811Nudixcoord: 252..431

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G009000.1Lsi06G009000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0044715 8-oxo-dGDP phosphatase activity