Lsi06G008250 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G008250
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	TF-B3 domain-containing protein
Location	chr06: 17553926 .. 17555975 (+)
RNA-Seq Expression	Lsi06G008250
Synteny	Lsi06G008250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGAGAAGATCACACAAAACCCAGTTGAACGTGAAGGAGAAGTCTATACACAAAACCCAATCGAACACGAAGGAGAAGACTATGGACAAAACGCAGTCGAACGCAGAGGAGAAGGAAAAAAAAAGAAGCGGAAAAGAAAAATGAAAGAGAAGGTCCACAAGCTCCAGAGATTTGCAAAGAGAAGCTGTAGAGAGAGAACGAAGGAGAAGAATGAAAGAGATGAAGATTTTGAGCGCCCTAATGAAAGAGGGGAGGATTTCGACGGGCGCCAAAATGTTTGTTTCTATAGGGTTTTATAATAAAAGCAATGCTGAAATGCTATAACTATACAATATATATCCTAATAAAAACATCTTACTTTATCATAAATTATTTGTTTTCCTTTCTCTTCTCCAAAATGATTTAGTTTCCTTCTTCTTATTGAAAACAACTTCATTCTCTTATAAATTTTACAAAATTGTATATTTCAATACCAACCAAAATCTTTTTCTCCTTCTACCATCTATTCTTTTTTTCTCTCTTCGGCAACTACAGGTTTAAGTTCAGTATAATAAAAAAAAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAAAAAACAAAAATGAGAGAAAAATAATAATAATCAATAGCTTTCCGGCCTATCACAACTATCCCCATTATAATGTTGCCATCAAGTTTTCACAAAGTTTCTGATTTCTTGTATTTGTCTATTAATCTATAGTAATTAAGTAGTGAAGTCTTCATACGTTGATTTTTTATATTTTATTCTTCTTTTTTCTTTTTTTTTTTTTTTGTAAAGAATGAATTTTAATTTACTATTTTTTATAATATTTTTATATTTTTTTTTAATTATATTTAAGAAATATTGTTGATTTCCACTATGGTTAAGGTACGTTACACATACAATAACAAAATAGTTGAGAAAGAGGTCTACGAAGGTGCTTCGATTATTTACGACATGAATAGTAAAAGCGTGAAGCAATCTGAGTCGTCAGAGCCGACAGGTCGTTTCAAAAATCAATTTCCAGGTTCAACTTCTACTTCCATAGTAAACGAGCAGCCTCCTGCAATCAACGAAGCTCCACCTAACAACAACAATGAAAATGTGGCGCCAGTGGCCGATGGAGCTCGAGATAGAATTTGGCCAATAGCAGTGATCGAAAATGTAATTGGAGCATGTAGTCGGCCATTTGTGAAGCAATTAACGAAAAGCGACATGTCAGATGGGCTAGGGCGATTGACACTTCAAAAGGAATTTGTGAATCGTCATTTGGTTCCAATGTTCAATGATGATGAGGATTTGATTCACGGGATTGATGTTATAGTTTTTGATAGTGAAGGGAGACAATACAACATGACGTTCAAGATTTGGTCTTCTAAGCTTTATGTGCTCACGAAAGGTTGGAAGGAGTTTTACCAGACTAATAATTTATTGCAATCTGGTGAGTATGTCACCGTTTGGATGTTCCGACATGTCGTTAATCACAAGCTTTGCTTTGCTATCATTCAAGGAAGGGTTGAAGGGCCTTGA

mRNA sequence

ATGAAGGAGAAGATCACACAAAACCCAGTTGAACGTGAAGGAGAAGTCTATACACAAAACCCAATCGAACACGAAGGAGAAGACTATGGACAAAACGCAGTCGAACGCAGAGGAGAAGGAAAAAAAAAGAAGCGGAAAAGAAAAATGAAAGAGAAGGTCCACAAGCTCCAGAGATTTGCAAAGAGAAGCTGTAGAGAGAGAACGAAGGAGAAGAATGAAAGAGATGAAGATTTTGAGCGCCCTAATGAAAGAGGGGAGGATTTCGACGGGCGCCAAAATGTACGTTACACATACAATAACAAAATAGTTGAGAAAGAGGTCTACGAAGGTGCTTCGATTATTTACGACATGAATAGTAAAAGCGTGAAGCAATCTGAGTCGTCAGAGCCGACAGGTCGTTTCAAAAATCAATTTCCAGGTTCAACTTCTACTTCCATAGTAAACGAGCAGCCTCCTGCAATCAACGAAGCTCCACCTAACAACAACAATGAAAATGTGGCGCCAGTGGCCGATGGAGCTCGAGATAGAATTTGGCCAATAGCAGTGATCGAAAATGTAATTGGAGCATGTAGTCGGCCATTTGTGAAGCAATTAACGAAAAGCGACATGTCAGATGGGCTAGGGCGATTGACACTTCAAAAGGAATTTGTGAATCGTCATTTGGTTCCAATGTTCAATGATGATGAGGATTTGATTCACGGGATTGATGTTATAGTTTTTGATAGTGAAGGGAGACAATACAACATGACGTTCAAGATTTGGTCTTCTAAGCTTTATGTGCTCACGAAAGGTTGGAAGGAGTTTTACCAGACTAATAATTTATTGCAATCTGGTGAGTATGTCACCGTTTGGATGTTCCGACATGTCGTTAATCACAAGCTTTGCTTTGCTATCATTCAAGGAAGGGTTGAAGGGCCTTGA

Coding sequence (CDS)

ATGAAGGAGAAGATCACACAAAACCCAGTTGAACGTGAAGGAGAAGTCTATACACAAAACCCAATCGAACACGAAGGAGAAGACTATGGACAAAACGCAGTCGAACGCAGAGGAGAAGGAAAAAAAAAGAAGCGGAAAAGAAAAATGAAAGAGAAGGTCCACAAGCTCCAGAGATTTGCAAAGAGAAGCTGTAGAGAGAGAACGAAGGAGAAGAATGAAAGAGATGAAGATTTTGAGCGCCCTAATGAAAGAGGGGAGGATTTCGACGGGCGCCAAAATGTACGTTACACATACAATAACAAAATAGTTGAGAAAGAGGTCTACGAAGGTGCTTCGATTATTTACGACATGAATAGTAAAAGCGTGAAGCAATCTGAGTCGTCAGAGCCGACAGGTCGTTTCAAAAATCAATTTCCAGGTTCAACTTCTACTTCCATAGTAAACGAGCAGCCTCCTGCAATCAACGAAGCTCCACCTAACAACAACAATGAAAATGTGGCGCCAGTGGCCGATGGAGCTCGAGATAGAATTTGGCCAATAGCAGTGATCGAAAATGTAATTGGAGCATGTAGTCGGCCATTTGTGAAGCAATTAACGAAAAGCGACATGTCAGATGGGCTAGGGCGATTGACACTTCAAAAGGAATTTGTGAATCGTCATTTGGTTCCAATGTTCAATGATGATGAGGATTTGATTCACGGGATTGATGTTATAGTTTTTGATAGTGAAGGGAGACAATACAACATGACGTTCAAGATTTGGTCTTCTAAGCTTTATGTGCTCACGAAAGGTTGGAAGGAGTTTTACCAGACTAATAATTTATTGCAATCTGGTGAGTATGTCACCGTTTGGATGTTCCGACATGTCGTTAATCACAAGCTTTGCTTTGCTATCATTCAAGGAAGGGTTGAAGGGCCTTGA

Protein sequence

MKEKITQNPVEREGEVYTQNPIEHEGEDYGQNAVERRGEGKKKKRKRKMKEKVHKLQRFAKRSCRERTKEKNERDEDFERPNERGEDFDGRQNVRYTYNNKIVEKEVYEGASIIYDMNSKSVKQSESSEPTGRFKNQFPGSTSTSIVNEQPPAINEAPPNNNNENVAPVADGARDRIWPIAVIENVIGACSRPFVKQLTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTFKIWSSKLYVLTKGWKEFYQTNNLLQSGEYVTVWMFRHVVNHKLCFAIIQGRVEGP

Homology

[Close]

BLAST of Lsi06G008250 vs. ExPASy Swiss-Prot
Match: Q9ZR14 (Putative B3 domain-containing protein At4g03170 OS=Arabidopsis thaliana OX=3702 GN=At4g03170 PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 4.2e-18
Identity = 71/225 (31.56%), Postives = 109/225 (48.44%), Query Frame = 0


Query: 83  ERGEDFDGRQNVRYTYNNKIVEKEVYEGASI-IYDMNSKSVKQS-------ESSEPTGRF 142
           +R ED D   ++    ++++++ E YE AS+ +   +  + KQS       E ++P  RF
Sbjct: 34  DREEDIDDEDDI----DDEVIDDEDYEVASLPLLQESQANQKQSREREEETEKNQPK-RF 93

Query: 143 KNQFPGSTSTSIVNEQPPAINEAPPNNNNENVAPVADGARDRIWPIAVIENVIGACSRPF 202
           K Q     +    +E+   + E       +      D    R++            SRP 
Sbjct: 94  KKQNMMKINIHDFSEETLRLIEVWYEAELDPQDIFGDNEVTRLF------------SRPI 153

Query: 203 VKQLTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTFKIW 262
            KQL  SD+      L L KE V   ++P   D E+ + GIDV V+  +G+   M FK+W
Sbjct: 154 KKQLMSSDVDKDQCMLMLSKEQVKEKMLPFLEDSENPVKGIDVSVYGPDGKVQQMEFKMW 213

Query: 263 S-SKLYVLTKGWKEFYQTNNLLQSGEYVTVWMFRHVVNHKLCFAI 299
           +  K  VLT GWK+F +   L  + ++VTVWMFRH+   KLCFAI
Sbjct: 214 NGDKTPVLTSGWKQFVEDYGLSMTCDFVTVWMFRHIKTRKLCFAI 241

[Close]

BLAST of Lsi06G008250 vs. ExPASy Swiss-Prot
Match: Q9ZR15 (Putative B3 domain-containing protein At4g03160 OS=Arabidopsis thaliana OX=3702 GN=At4g03160 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.1e-13
Identity = 38/107 (35.51%), Postives = 58/107 (54.21%), Query Frame = 0


Query: 192 RPFVKQLTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTF 251
           R   +QL  SD+ D   RL L KE V + +  +   ++    G++V V+   G  + M  
Sbjct: 63  RKVAEQLMDSDVKDNQYRLMLGKEPVKKMMDALGKTEKLGTKGLNVSVYGPNGENHKMVL 122

Query: 252 KIWSSKLYVLTKGWKEFYQTNNLLQSGEYVTVWMFRHVVNHKLCFAI 299
           KIW     VLT GWK F ++  L +  +++T+WMFRH    ++CFAI
Sbjct: 123 KIWIKGTPVLTSGWKNFVKSYKLEKHVDFLTIWMFRHKKTREICFAI 169

[Close]

BLAST of Lsi06G008250 vs. ExPASy Swiss-Prot
Match: Q9LM90 (B3 domain-containing protein At1g20600 OS=Arabidopsis thaliana OX=3702 GN=At1g20600 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 9.0e-13
Identity = 37/93 (39.78%), Postives = 52/93 (55.91%), Query Frame = 0


Query: 191 SRPFVKQLTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMT 250
           SRP  KQL  SD+     RL L KE V   ++P   + ED + G+DV V+  +G    M 
Sbjct: 122 SRPIKKQLMSSDVDTDQSRLMLSKEQVKEKMLPFLEESEDPVKGVDVSVYGPDGEVQQMK 181

Query: 251 FKIWS-SKLYVLTKGWKEFYQTNNLLQSGEYVT 283
           FK+W+  K  VLT GW +F     LL++ ++VT
Sbjct: 182 FKMWNGDKTPVLTSGWMQFVADYGLLKTSDFVT 214

[Close]

BLAST of Lsi06G008250 vs. ExPASy Swiss-Prot
Match: P82280 (AP2/ERF and B3 domain-containing transcription repressor RAV2 OS=Arabidopsis thaliana OX=3702 GN=RAV2 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 4.1e-05
Identity = 30/90 (33.33%), Postives = 48/90 (53.33%), Query Frame = 0


Query: 194 FVKQLTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTFKI 253
           F K +T SD+   L RL + K+   +H  P+ +    +  G+ +   D  G+ +   +  
Sbjct: 188 FEKAVTPSDVGK-LNRLVIPKQHAEKHF-PLPSPSPAVTKGVLINFEDVNGKVWRFRYSY 247

Query: 254 W-SSKLYVLTKGWKEFYQTNNLLQSGEYVT 283
           W SS+ YVLTKGW  F +  N L++G+ VT
Sbjct: 248 WNSSQSYVLTKGWSRFVKEKN-LRAGDVVT 274

[Close]

BLAST of Lsi06G008250 vs. ExPASy TrEMBL
Match: A0A5D3BAS8 (Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold78209G00040 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 5.2e-64
Identity = 133/230 (57.83%), Postives = 163/230 (70.87%), Query Frame = 0


Query: 94  VRYTYNNKIVEKEVYEGASIIYDMNSKSVKQSESSEPTGRFKNQFPGSTSTSIVNEQPPA 153
           V Y YNNKIV+KEVYE    + +M        +SSEP     NQFP STS S+V++QPP 
Sbjct: 4   VGYIYNNKIVKKEVYEACLALCEM-------KKSSEPFEE-PNQFPSSTSISMVDDQPPV 63

Query: 154 INEAPP-----------------NNNNENVAP-VADGARDRIWPIAVIENVIGACSRPFV 213
           +NE PP                 NNNNENVA  VAD    R+ P+AVI+N+IG CS PFV
Sbjct: 64  VNEQPPIVNEHAPVVNEALLNNNNNNNENVAAVVADRDLGRLPPMAVIQNIIGECSPPFV 123

Query: 214 KQLTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTFKIWS 273
           KQLTK+D++D  GRL L KEFVNR+LVPMFN DE L +GI V V+DSEGR+Y+MTF++W+
Sbjct: 124 KQLTKTDVTDSQGRLALHKEFVNRNLVPMFNGDEILTNGISVTVYDSEGREYDMTFRLWT 183

Query: 274 SKLYVLTKGWKEFYQTNNLLQSGEYVTVWMFRHVVNHKLCFAIIQGRVEG 306
           SKLYVLTK WK+FY  N L +  E++T+WMFRHVVNHKLCFAI++G VEG
Sbjct: 184 SKLYVLTKSWKKFYHDNGLTRPDEFITLWMFRHVVNHKLCFAIMRGNVEG 225

[Close]

BLAST of Lsi06G008250 vs. ExPASy TrEMBL
Match: A0A0A0L7L0 (TF-B3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G073910 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 1.5e-63
Identity = 135/225 (60.00%), Postives = 164/225 (72.89%), Query Frame = 0


Query: 93  NVRYTYNNKIVEKEVYEGASIIYDMN-SKSVKQSESSEPTGRFKNQFPGSTSTSIVNEQP 152
           +V YTYNNKIV ++VY+ + I+YDM  SKSV+  E  EP  RF NQ   STSTS+VNEQ 
Sbjct: 3   DVHYTYNNKIVAEDVYKASLILYDMALSKSVEPFE--EPIDRFNNQL-SSTSTSLVNEQS 62

Query: 153 PAINEAPP------------NNNNENVAPVADGARD--RIWPIAVIENVIGACSRPFVKQ 212
           P +NE  P            N NNENVA      RD  R  P+AVI+N+IG CS PFVKQ
Sbjct: 63  PVVNEHAPVVNEVLPNNDDDNGNNENVAAAVVVGRDQGRFPPMAVIQNIIGECSPPFVKQ 122

Query: 213 LTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTFKIWSSK 272
           LTK+D++D  GRL L KEFVN +L PMFNDDEDL  GI VIV+D EGR+Y+M FK+W+SK
Sbjct: 123 LTKTDVTDNQGRLALHKEFVNLNLDPMFNDDEDLEDGISVIVYDIEGREYDMIFKLWASK 182

Query: 273 LYVLTKGWKEFYQTNNLLQSGEYVTVWMFRHVVNHKLCFAIIQGR 303
           LYVLTK WKEFY+TN+L Q GE+++VWMFRHVV  KLCFAI++G+
Sbjct: 183 LYVLTKSWKEFYKTNDLTQPGEFISVWMFRHVVTQKLCFAIMRGQ 224

[Close]

BLAST of Lsi06G008250 vs. ExPASy TrEMBL
Match: A0A0A0LC65 (TF-B3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G609250 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 1.6e-41
Identity = 79/125 (63.20%), Postives = 103/125 (82.40%), Query Frame = 0


Query: 180 IAVIENVIGACSRPFVKQLTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIV 239
           +AVI+N+IG CS PFVKQL K+D+++  GRL L KEFV R+L+PMFN  E+L +GI V V
Sbjct: 1   MAVIQNIIGECSPPFVKQLMKTDVTESQGRLALHKEFVTRNLIPMFNRGENLKNGISVTV 60

Query: 240 FDSEGRQYNMTFKIWSSKLYVLTKGWKEFYQTNNLLQSGEYVTVWMFRHVVNHKLCFAII 299
           +DSEGR+Y+M FK W+SKLYVLTK W +FY++NNL + GE+++VWMFRHVVN KLCFAI+
Sbjct: 61  YDSEGREYDMIFKFWTSKLYVLTKSWNKFYKSNNLTRPGEFISVWMFRHVVNRKLCFAIM 120

Query: 300 QGRVE 305
           +G  E
Sbjct: 121 RGDAE 125

[Close]

BLAST of Lsi06G008250 vs. ExPASy TrEMBL
Match: A0A6J1CTE7 (putative B3 domain-containing protein At4g03170 OS=Momordica charantia OX=3673 GN=LOC111014582 PE=4 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 5.7e-34
Identity = 97/232 (41.81%), Postives = 129/232 (55.60%), Query Frame = 0


Query: 99  NNKIVEKEVYEGASIIYDMNSKSVKQSESSEPTGRFKNQFPGSTSTSIVNEQPPAINEAP 158
           N K  + EVYE A  +Y M+  +        PT R      G T      ++   IN+  
Sbjct: 6   NKKEEDSEVYEAAMTLYMMSKSNEGSVTRFLPTSRLTTFPQGVTLFKKPKKKRHYINDNV 65

Query: 159 PNNNNENV-----------------APV------ADGARDR-IWPIAVIENVIGACSRPF 218
            NNNN NV                 AP        DG   R + P+  I +VIG CSR +
Sbjct: 66  HNNNNVNVDEERQPSSSRNKQVNQEAPPDPPNNNNDGEERRGMPPVPAIRDVIGQCSRAY 125

Query: 219 VKQLTKSDMSDGLGRLTLQKEFVNRHLVPMFN---DDEDLIHGIDVIVFDSEGRQYNMTF 278
            K+LT SD+++  GRL + KEFV ++L PMFN   ++E+L  GI+V V++ EGR++ MTF
Sbjct: 126 EKRLTVSDVAEAQGRLAIGKEFVTKYLAPMFNNKDENENLREGIEVTVYNREGREFGMTF 185

Query: 279 KIWSSKLYVLTKGWKEFYQTNNL--LQSGEYVTVWMFRHVVNHKLCFAIIQG 302
           KIW+SKLYVL KGWK F + NNL    +GE++T+WMFRH   H LCFAII G
Sbjct: 186 KIWASKLYVLMKGWKPFCKANNLTHAHAGEFLTLWMFRHRRTHGLCFAIILG 237

[Close]

BLAST of Lsi06G008250 vs. ExPASy TrEMBL
Match: A0A2P6RSD2 (Putative transcription factor B3-Domain family OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0120751 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 3.9e-27
Identity = 66/159 (41.51%), Postives = 92/159 (57.86%), Query Frame = 0


Query: 140 GSTSTSIVNEQPPAINEAPPNNNNENVAPVADGARDRIWPIAVIENVIGACSRPFVKQLT 199
           G    ++  E+  A+NEA P           D     + P+  +  +I  CS PF KQLT
Sbjct: 92  GPKRKAVHTEEQEAMNEAKPQQG----LFYGDYTPPNLPPVPSLHKLIEICSEPFEKQLT 151

Query: 200 KSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTFKIWSSKLY 259
            SD+ D   RL + KE V +HL+P+ N+ EDL  GIDV  +D  G++Y M FK W SK++
Sbjct: 152 CSDVRDDQSRLAMSKEDVQKHLMPLLNESEDLNEGIDVTTYDLAGKEYPMKFKTWVSKIH 211

Query: 260 VLTKGWKEFYQTNNLLQSGEYVTVWMFRHVVNHKLCFAI 299
           VLT GWK F + + L+Q+  +VTVWMFR++    LCF I
Sbjct: 212 VLTGGWKAFCRDHQLVQTQHFVTVWMFRNIATGNLCFVI 246

[Close]

BLAST of Lsi06G008250 vs. NCBI nr
Match: KAA0043114.1 (putative B3 domain-containing protein [Cucumis melo var. makuwa] >TYJ96217.1 putative B3 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 254.6 bits (649), Expect = 1.1e-63
Identity = 133/230 (57.83%), Postives = 163/230 (70.87%), Query Frame = 0


Query: 94  VRYTYNNKIVEKEVYEGASIIYDMNSKSVKQSESSEPTGRFKNQFPGSTSTSIVNEQPPA 153
           V Y YNNKIV+KEVYE    + +M        +SSEP     NQFP STS S+V++QPP 
Sbjct: 4   VGYIYNNKIVKKEVYEACLALCEM-------KKSSEPFEE-PNQFPSSTSISMVDDQPPV 63

Query: 154 INEAPP-----------------NNNNENVAP-VADGARDRIWPIAVIENVIGACSRPFV 213
           +NE PP                 NNNNENVA  VAD    R+ P+AVI+N+IG CS PFV
Sbjct: 64  VNEQPPIVNEHAPVVNEALLNNNNNNNENVAAVVADRDLGRLPPMAVIQNIIGECSPPFV 123

Query: 214 KQLTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTFKIWS 273
           KQLTK+D++D  GRL L KEFVNR+LVPMFN DE L +GI V V+DSEGR+Y+MTF++W+
Sbjct: 124 KQLTKTDVTDSQGRLALHKEFVNRNLVPMFNGDEILTNGISVTVYDSEGREYDMTFRLWT 183

Query: 274 SKLYVLTKGWKEFYQTNNLLQSGEYVTVWMFRHVVNHKLCFAIIQGRVEG 306
           SKLYVLTK WK+FY  N L +  E++T+WMFRHVVNHKLCFAI++G VEG
Sbjct: 184 SKLYVLTKSWKKFYHDNGLTRPDEFITLWMFRHVVNHKLCFAIMRGNVEG 225

[Close]

BLAST of Lsi06G008250 vs. NCBI nr
Match: KGN56101.1 (hypothetical protein Csa_010492 [Cucumis sativus])

HSP 1 Score: 253.1 bits (645), Expect = 3.1e-63
Identity = 135/225 (60.00%), Postives = 164/225 (72.89%), Query Frame = 0


Query: 93  NVRYTYNNKIVEKEVYEGASIIYDMN-SKSVKQSESSEPTGRFKNQFPGSTSTSIVNEQP 152
           +V YTYNNKIV ++VY+ + I+YDM  SKSV+  E  EP  RF NQ   STSTS+VNEQ 
Sbjct: 3   DVHYTYNNKIVAEDVYKASLILYDMALSKSVEPFE--EPIDRFNNQL-SSTSTSLVNEQS 62

Query: 153 PAINEAPP------------NNNNENVAPVADGARD--RIWPIAVIENVIGACSRPFVKQ 212
           P +NE  P            N NNENVA      RD  R  P+AVI+N+IG CS PFVKQ
Sbjct: 63  PVVNEHAPVVNEVLPNNDDDNGNNENVAAAVVVGRDQGRFPPMAVIQNIIGECSPPFVKQ 122

Query: 213 LTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTFKIWSSK 272
           LTK+D++D  GRL L KEFVN +L PMFNDDEDL  GI VIV+D EGR+Y+M FK+W+SK
Sbjct: 123 LTKTDVTDNQGRLALHKEFVNLNLDPMFNDDEDLEDGISVIVYDIEGREYDMIFKLWASK 182

Query: 273 LYVLTKGWKEFYQTNNLLQSGEYVTVWMFRHVVNHKLCFAIIQGR 303
           LYVLTK WKEFY+TN+L Q GE+++VWMFRHVV  KLCFAI++G+
Sbjct: 183 LYVLTKSWKEFYKTNDLTQPGEFISVWMFRHVVTQKLCFAIMRGQ 224

[Close]

BLAST of Lsi06G008250 vs. NCBI nr
Match: XP_031736688.1 (uncharacterized protein LOC116402065 [Cucumis sativus])

HSP 1 Score: 248.4 bits (633), Expect = 7.8e-62
Identity = 130/221 (58.82%), Postives = 164/221 (74.21%), Query Frame = 0


Query: 94  VRYTYNNKIVEKEVYEGASIIYDM-NSKSVKQSESSEPTGRFKNQFPGSTSTSIVNEQP- 153
           VRYTYNNKIVEK VYE +  +  M NS+ +K  E  +P  R  N+F  STS SI+N+QP 
Sbjct: 4   VRYTYNNKIVEKHVYEASLALCHMKNSRCLKPFE--KPVTRLNNRFSSSTSISILNKQPP 63

Query: 154 ------PAINEAPPNNNNENVAPVADGARD--RIWPIAVIENVIGACSRPFVKQLTKSDM 213
                 P +NEA PNNNNENVA VA   RD  R+ P+AVI+N+IG CS PFVKQL K+D+
Sbjct: 64  ILIERAPIVNEALPNNNNENVA-VAVAGRDWGRVPPMAVIQNIIGECSPPFVKQLMKTDV 123

Query: 214 SDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTFKIWSSKLYVLTK 273
           ++  GRL L KEFV R+L+PMFN  E+L +GI V V+DSEGR+Y+M FK W+SKLYVLTK
Sbjct: 124 TESQGRLALHKEFVTRNLIPMFNRGENLKNGISVTVYDSEGREYDMIFKFWTSKLYVLTK 183

Query: 274 GWKEFYQTNNLLQSGEYVTVWMFRHVVNHKLCFAIIQGRVE 305
            W +FY++NNL + GE+++VWMFRHVVN KLCFAI++G  E
Sbjct: 184 SWNKFYKSNNLTRPGEFISVWMFRHVVNRKLCFAIMRGDAE 221

[Close]

BLAST of Lsi06G008250 vs. NCBI nr
Match: KAG6589940.1 (putative B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 205.3 bits (521), Expect = 7.5e-49
Identity = 109/218 (50.00%), Postives = 144/218 (66.06%), Query Frame = 0


Query: 94  VRYTYNNKIVEKEVYEGASIIYDMNSKSVKQSESSEPTGRFKNQFPGSTSTSIVNEQPPA 153
           V Y +NN+I+EK+VYE A I + +        +  EP+     Q  GSTS  + +E    
Sbjct: 4   VHYRHNNRIIEKDVYETARIFHCL----CTGEQFPEPSYSSDEQVSGSTSGDMDDE---- 63

Query: 154 INEAPPNNNNENVAPVADGARDRIWPI-----AVIENVIGACSRPFVKQLTKSDMSDGLG 213
            NEAP  N +E+VA   +   + + P+     AVI N+IGA S P+ K+LTKSD+S+G G
Sbjct: 64  -NEAPSINGDEDVALEVEEVAEVVAPVVIPALAVIMNIIGARSPPWEKRLTKSDVSEGQG 123

Query: 214 RLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTFKIWSSKLYVLTKGWKEF 273
           RL ++KEFV  HL+PMFN DED   GIDV V++ EGRQY+M FK+W+SKLYVLTKGW +F
Sbjct: 124 RLIVRKEFVTNHLLPMFNADEDPSEGIDVTVYNGEGRQYDMQFKLWASKLYVLTKGWNDF 183

Query: 274 YQTNNLLQSGEYVTVWMFRHVVNHKLCFAIIQGRVEGP 307
           Y+TNNL   G YVT+WMFRHV  HK+CFA+  G+   P
Sbjct: 184 YKTNNLTDPGLYVTLWMFRHVETHKICFAVTAGQFGEP 212

[Close]

BLAST of Lsi06G008250 vs. NCBI nr
Match: KAG7023605.1 (putative B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 185.7 bits (470), Expect = 6.2e-43
Identity = 89/157 (56.69%), Postives = 115/157 (73.25%), Query Frame = 0


Query: 155 NEAPPNNNNENVAPVADGARDRIWPI-----AVIENVIGACSRPFVKQLTKSDMSDGLGR 214
           NEAP  N +E+VA   +   + + P+     AVI N+IGA S P+ K+LTKSD+S+G GR
Sbjct: 5   NEAPSINGDEDVALEVEEVAEVVAPVVIPALAVIMNIIGARSPPWEKRLTKSDVSEGQGR 64

Query: 215 LTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTFKIWSSKLYVLTKGWKEFY 274
           L ++KEFV  HL+PMFN DED   GIDV V++ EGRQY+M FK+W+SKLYVLTKGW +FY
Sbjct: 65  LIVRKEFVTNHLLPMFNADEDPSEGIDVTVYNGEGRQYDMQFKLWASKLYVLTKGWNDFY 124

Query: 275 QTNNLLQSGEYVTVWMFRHVVNHKLCFAIIQGRVEGP 307
           +TNNL + G YVT+WMFRHV  HK+CFA+  G+   P
Sbjct: 125 KTNNLTEPGLYVTLWMFRHVETHKICFAVTAGQFGEP 161

[Close]

BLAST of Lsi06G008250 vs. TAIR 10
Match: AT4G03170.1 (AP2/B3-like transcriptional factor family protein )

HSP 1 Score: 93.6 bits (231), Expect = 3.0e-19
Identity = 71/225 (31.56%), Postives = 109/225 (48.44%), Query Frame = 0


Query: 83  ERGEDFDGRQNVRYTYNNKIVEKEVYEGASI-IYDMNSKSVKQS-------ESSEPTGRF 142
           +R ED D   ++    ++++++ E YE AS+ +   +  + KQS       E ++P  RF
Sbjct: 34  DREEDIDDEDDI----DDEVIDDEDYEVASLPLLQESQANQKQSREREEETEKNQPK-RF 93

Query: 143 KNQFPGSTSTSIVNEQPPAINEAPPNNNNENVAPVADGARDRIWPIAVIENVIGACSRPF 202
           K Q     +    +E+   + E       +      D    R++            SRP 
Sbjct: 94  KKQNMMKINIHDFSEETLRLIEVWYEAELDPQDIFGDNEVTRLF------------SRPI 153

Query: 203 VKQLTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTFKIW 262
            KQL  SD+      L L KE V   ++P   D E+ + GIDV V+  +G+   M FK+W
Sbjct: 154 KKQLMSSDVDKDQCMLMLSKEQVKEKMLPFLEDSENPVKGIDVSVYGPDGKVQQMEFKMW 213

Query: 263 S-SKLYVLTKGWKEFYQTNNLLQSGEYVTVWMFRHVVNHKLCFAI 299
           +  K  VLT GWK+F +   L  + ++VTVWMFRH+   KLCFAI
Sbjct: 214 NGDKTPVLTSGWKQFVEDYGLSMTCDFVTVWMFRHIKTRKLCFAI 241

[Close]

BLAST of Lsi06G008250 vs. TAIR 10
Match: AT4G03160.1 (BEST Arabidopsis thaliana protein match is: AP2/B3-like transcriptional factor family protein (TAIR:AT4G03170.1); Has 46 Blast hits to 46 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 8; Fungi - 4; Plants - 29; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink). )

HSP 1 Score: 77.4 bits (189), Expect = 2.2e-14
Identity = 38/107 (35.51%), Postives = 58/107 (54.21%), Query Frame = 0


Query: 192 RPFVKQLTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTF 251
           R   +QL  SD+ D   RL L KE V + +  +   ++    G++V V+   G  + M  
Sbjct: 63  RKVAEQLMDSDVKDNQYRLMLGKEPVKKMMDALGKTEKLGTKGLNVSVYGPNGENHKMVL 122

Query: 252 KIWSSKLYVLTKGWKEFYQTNNLLQSGEYVTVWMFRHVVNHKLCFAI 299
           KIW     VLT GWK F ++  L +  +++T+WMFRH    ++CFAI
Sbjct: 123 KIWIKGTPVLTSGWKNFVKSYKLEKHVDFLTIWMFRHKKTREICFAI 169

[Close]

BLAST of Lsi06G008250 vs. TAIR 10
Match: AT1G20600.1 (AP2/B3-like transcriptional factor family protein )

HSP 1 Score: 75.9 bits (185), Expect = 6.4e-14
Identity = 37/93 (39.78%), Postives = 52/93 (55.91%), Query Frame = 0


Query: 191 SRPFVKQLTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMT 250
           SRP  KQL  SD+     RL L KE V   ++P   + ED + G+DV V+  +G    M 
Sbjct: 122 SRPIKKQLMSSDVDTDQSRLMLSKEQVKEKMLPFLEESEDPVKGVDVSVYGPDGEVQQMK 181

Query: 251 FKIWS-SKLYVLTKGWKEFYQTNNLLQSGEYVT 283
           FK+W+  K  VLT GW +F     LL++ ++VT
Sbjct: 182 FKMWNGDKTPVLTSGWMQFVADYGLLKTSDFVT 214

[Close]

BLAST of Lsi06G008250 vs. TAIR 10
Match: AT1G68840.1 (related to ABI3/VP1 2 )

HSP 1 Score: 50.4 bits (119), Expect = 2.9e-06
Identity = 30/90 (33.33%), Postives = 48/90 (53.33%), Query Frame = 0


Query: 194 FVKQLTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTFKI 253
           F K +T SD+   L RL + K+   +H  P+ +    +  G+ +   D  G+ +   +  
Sbjct: 188 FEKAVTPSDVGK-LNRLVIPKQHAEKHF-PLPSPSPAVTKGVLINFEDVNGKVWRFRYSY 247

Query: 254 W-SSKLYVLTKGWKEFYQTNNLLQSGEYVT 283
           W SS+ YVLTKGW  F +  N L++G+ VT
Sbjct: 248 WNSSQSYVLTKGWSRFVKEKN-LRAGDVVT 274

[Close]

BLAST of Lsi06G008250 vs. TAIR 10
Match: AT1G68840.2 (related to ABI3/VP1 2 )

HSP 1 Score: 50.4 bits (119), Expect = 2.9e-06
Identity = 30/90 (33.33%), Postives = 48/90 (53.33%), Query Frame = 0


Query: 194 FVKQLTKSDMSDGLGRLTLQKEFVNRHLVPMFNDDEDLIHGIDVIVFDSEGRQYNMTFKI 253
           F K +T SD+   L RL + K+   +H  P+ +    +  G+ +   D  G+ +   +  
Sbjct: 188 FEKAVTPSDVGK-LNRLVIPKQHAEKHF-PLPSPSPAVTKGVLINFEDVNGKVWRFRYSY 247

Query: 254 W-SSKLYVLTKGWKEFYQTNNLLQSGEYVT 283
           W SS+ YVLTKGW  F +  N L++G+ VT
Sbjct: 248 WNSSQSYVLTKGWSRFVKEKN-LRAGDVVT 274

The following BLAST results are available for this feature:

BLAST of Lsi06G008250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZR14	4.2e-18	31.56	Putative B3 domain-containing protein At4g03170 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9ZR15	3.1e-13	35.51	Putative B3 domain-containing protein At4g03160 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9LM90	9.0e-13	39.78	B3 domain-containing protein At1g20600 OS=Arabidopsis thaliana OX=3702 GN=At1g20...	[more]
P82280	4.1e-05	33.33	AP2/ERF and B3 domain-containing transcription repressor RAV2 OS=Arabidopsis tha...	[more]

BLAST of Lsi06G008250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BAS8	5.2e-64	57.83	Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A0A0L7L0	1.5e-63	60.00	TF-B3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G073910 PE=4 ...	[more]
A0A0A0LC65	1.6e-41	63.20	TF-B3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G609250 PE=4 ...	[more]
A0A6J1CTE7	5.7e-34	41.81	putative B3 domain-containing protein At4g03170 OS=Momordica charantia OX=3673 G...	[more]
A0A2P6RSD2	3.9e-27	41.51	Putative transcription factor B3-Domain family OS=Rosa chinensis OX=74649 GN=Rch...	[more]

BLAST of Lsi06G008250 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0043114.1	1.1e-63	57.83	putative B3 domain-containing protein [Cucumis melo var. makuwa] >TYJ96217.1 put...	[more]
KGN56101.1	3.1e-63	60.00	hypothetical protein Csa_010492 [Cucumis sativus]	[more]
XP_031736688.1	7.8e-62	58.82	uncharacterized protein LOC116402065 [Cucumis sativus]	[more]
KAG6589940.1	7.5e-49	50.00	putative B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. so...	[more]
KAG7023605.1	6.2e-43	56.69	putative B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. ar...	[more]

BLAST of Lsi06G008250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03170.1	3.0e-19	31.56	AP2/B3-like transcriptional factor family protein	[more]
AT4G03160.1	2.2e-14	35.51	BEST Arabidopsis thaliana protein match is: AP2/B3-like transcriptional factor f...	[more]
AT1G20600.1	6.4e-14	39.78	AP2/B3-like transcriptional factor family protein	[more]
AT1G68840.1	2.9e-06	33.33	related to ABI3/VP1 2	[more]
AT1G68840.2	2.9e-06	33.33	related to ABI3/VP1 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003340	B3 DNA binding domain	SMART	SM01019	B3_2	coord: 194..302 e-value: 1.2E-6 score: 38.1
IPR003340	B3 DNA binding domain	PFAM	PF02362	B3	coord: 194..296 e-value: 4.2E-10 score: 39.4
IPR003340	B3 DNA binding domain	PROSITE	PS50863	B3	coord: 195..302 score: 9.643255
IPR003340	B3 DNA binding domain	CDD	cd10017	B3_DNA	coord: 194..299 e-value: 6.31169E-16 score: 70.0493
IPR015300	DNA-binding pseudobarrel domain superfamily	GENE3D	2.40.330.10		coord: 188..302 e-value: 5.1E-21 score: 76.4
IPR015300	DNA-binding pseudobarrel domain superfamily	SUPERFAMILY	101936	DNA-binding pseudobarrel domain	coord: 194..292
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 124..146
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 39..62
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 63..91
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..91
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 17..38
None	No IPR available	PANTHER	PTHR31541:SF28	B3 DOMAIN PLANT PROTEIN	coord: 41..300
IPR005508	B3 domain-containing protein At2g31720-like	PANTHER	PTHR31541	B3 DOMAIN PLANT PROTEIN-RELATED	coord: 41..300

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G008250.1	Lsi06G008250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003677	DNA binding