Lsi06G007860 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G007860
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein kinase domain-containing protein
Locationchr06: 16007348 .. 16009157 (+)
RNA-Seq ExpressionLsi06G007860
SyntenyLsi06G007860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCCCTAAAACCTCTATAAAAATCCCCCCTTACCCAACTGATTCCCCCAAAAGCACTCCCACTTCAGACTACAGAGAAAGAGAGAGAGAGAGAGAAACAAATAAATTCTTCAAGAATTTCTAGAGAGAAAAAAAAAAAAACCAAATCCCCCAAATTCCCAAATCCCAAATCGAAGAAGAAATCTTCAACAATGGGCGACATGATCGTGAACAGCCAATACCAAATCCTTGAGGAGATCGGAAGAGGACGATTCGGAACCATCACTCGTTGCTTTTGCCCAATCTCCAACAAGTTCTTCGCCTGCAAAACCATCGCCAAAAACGATCTCATCGATGAAACTGACAAAGAATGCCTTGAAAAAGAGCCCAAAATCATGGCTCTTCTCCCTCCCCATCCCAACATTCTTCAGTTAATCGACGTCCTCGAGAACGATGATTACCTCTGTTTGATCTCCGAACTCTGTGATTCCCTTTCCCTTTACGATCGGATCATCCGTCGTCCCTTTTCCGAATCTGAAGCCGTCATCGTTATGAAGCAGCTTCTCGAAGCCCTCGCCCACTGTCATTCCCATGGCGTCGTTCATCGCGATGTCAAGCCCGATAATGTGCTCTTCGATTCCAGAAACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGTGATAAAGACGGATTTATGTTCGGCGTTGTCGGAACGCCTTATTACGTCGCGCCGGAGGTTTTGAGAGGATCTGAGTACGATCGGAAGGTCGATGTGTGGAGCGCCGGTGTGATTCTTTACACCATGTTGGCCGGATTTCCCCCGTTCTATGGTGACTCTGCGGCGGAGGTCTTTGAGGCTGTTCTTAGAGGAAATCTCAGATTTCCGACTAGGGTCTTTAGGTCGGTTTCTTCGGCGGCTAAAGATCTGATGAAGAAGATGATCTGTCGAGATTTTTCTAGAAGATTCTCAGCTGAACAAGCTCTCAGTAAGTCTAATTCTCTTCTGATTTCTCTTTGATCTGATTCTTCCATGTTTGTTTGTAAATCTTTAACATTAGAAGAAACCCATTACATATTTATTTGTTTGGTTGGTGAGAAAATTTCTGTATGAACATGGAGAAACAATCTTTTATCTGTTTTCTTATGGGAATTGAAAATTGTTGTTTACAGGACACCCATGGATTTTGAGTGGAGGAGAAGGAACATCTTCAGTTGAATAATTTGATTTAGAACTTTTTGACTAAACCCAAAAAAGAGAGAATATAAAATGAAAGGATGCTATTGAATAGAAGCATTCTAAAGCCAGTTTTGCATTCCAGTACATAATCCCCTGCTCTGCCTTCTTCCCCACTATGTGAATAAGAAACCAGAAACCTGAACCCTTCAGTTCAGACTCCATTAATGGCTGCCTGCTCCATCTACAAGGTTCTAGAGTTGTTGATTTGAGACTGTTCTGCTGCTGTCAATATCTTCTTTCTTTTTTGATTTCTTCAAAGCTGTCTTTGAAGCTTTTTTTCATTTTGCTTTTCCTTTTTGGTTTCTTGTTAGGTAAAAAAAATGGAATTTGGGAATCCTTTCATTCCCTCCCAAGCCTTTTTCTTTTTGTTGTATAAAGTGTTTGGGAAGTGAGAAAGTCCCACAAAAAATGGAAAAAAAAAAAAAAAAAAAGGAGAGAGAAAAGAGAGAGAGAAAGAGAAGTTGTATTCTAATTAGTAGTTGTGTTTTTTGTTTTGGTTTTTCTAATTTTGAAGGATGTAATGTTAATGTGATTGTGATGTGTTGAATGCAATATGCTGGGAATATAAGTGATGTTTTTAGTGTAAA

mRNA sequence

ATCCCCTAAAACCTCTATAAAAATCCCCCCTTACCCAACTGATTCCCCCAAAAGCACTCCCACTTCAGACTACAGAGAAAGAGAGAGAGAGAGAGAAACAAATAAATTCTTCAAGAATTTCTAGAGAGAAAAAAAAAAAAACCAAATCCCCCAAATTCCCAAATCCCAAATCGAAGAAGAAATCTTCAACAATGGGCGACATGATCGTGAACAGCCAATACCAAATCCTTGAGGAGATCGGAAGAGGACGATTCGGAACCATCACTCGTTGCTTTTGCCCAATCTCCAACAAGTTCTTCGCCTGCAAAACCATCGCCAAAAACGATCTCATCGATGAAACTGACAAAGAATGCCTTGAAAAAGAGCCCAAAATCATGGCTCTTCTCCCTCCCCATCCCAACATTCTTCAGTTAATCGACGTCCTCGAGAACGATGATTACCTCTGTTTGATCTCCGAACTCTGTGATTCCCTTTCCCTTTACGATCGGATCATCCGTCGTCCCTTTTCCGAATCTGAAGCCGTCATCGTTATGAAGCAGCTTCTCGAAGCCCTCGCCCACTGTCATTCCCATGGCGTCGTTCATCGCGATGTCAAGCCCGATAATGTGCTCTTCGATTCCAGAAACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGTGATAAAGACGGATTTATGTTCGGCGTTGTCGGAACGCCTTATTACGTCGCGCCGGAGGTTTTGAGAGGATCTGAGTACGATCGGAAGGTCGATGTGTGGAGCGCCGGTGTGATTCTTTACACCATGTTGGCCGGATTTCCCCCGTTCTATGGTGACTCTGCGGCGGAGGTCTTTGAGGCTGTTCTTAGAGGAAATCTCAGATTTCCGACTAGGGTCTTTAGGTCGGTTTCTTCGGCGGCTAAAGATCTGATGAAGAAGATGATCTGTCGAGATTTTTCTAGAAGATTCTCAGCTGAACAAGCTCTCAGACACCCATGGATTTTGAGTGGAGGAGAAGGAACATCTTCAGTTGAATAATTTGATTTAGAACTTTTTGACTAAACCCAAAAAAGAGAGAATATAAAATGAAAGGATGCTATTGAATAGAAGCATTCTAAAGCCAGTTTTGCATTCCAGTACATAATCCCCTGCTCTGCCTTCTTCCCCACTATGTGAATAAGAAACCAGAAACCTGAACCCTTCAGTTCAGACTCCATTAATGGCTGCCTGCTCCATCTACAAGGTTCTAGAGTTGTTGATTTGAGACTGTTCTGCTGCTGTCAATATCTTCTTTCTTTTTTGATTTCTTCAAAGCTGTCTTTGAAGCTTTTTTTCATTTTGCTTTTCCTTTTTGGTTTCTTGTTAGGTAAAAAAAATGGAATTTGGGAATCCTTTCATTCCCTCCCAAGCCTTTTTCTTTTTGTTGTATAAAGTGTTTGGGAAGTGAGAAAGTCCCACAAAAAATGGAAAAAAAAAAAAAAAAAAAGGAGAGAGAAAAGAGAGAGAGAAAGAGAAGTTGTATTCTAATTAGTAGTTGTGTTTTTTGTTTTGGTTTTTCTAATTTTGAAGGATGTAATGTTAATGTGATTGTGATGTGTTGAATGCAATATGCTGGGAATATAAGTGATGTTTTTAGTGTAAA

Coding sequence (CDS)

ATGGGCGACATGATCGTGAACAGCCAATACCAAATCCTTGAGGAGATCGGAAGAGGACGATTCGGAACCATCACTCGTTGCTTTTGCCCAATCTCCAACAAGTTCTTCGCCTGCAAAACCATCGCCAAAAACGATCTCATCGATGAAACTGACAAAGAATGCCTTGAAAAAGAGCCCAAAATCATGGCTCTTCTCCCTCCCCATCCCAACATTCTTCAGTTAATCGACGTCCTCGAGAACGATGATTACCTCTGTTTGATCTCCGAACTCTGTGATTCCCTTTCCCTTTACGATCGGATCATCCGTCGTCCCTTTTCCGAATCTGAAGCCGTCATCGTTATGAAGCAGCTTCTCGAAGCCCTCGCCCACTGTCATTCCCATGGCGTCGTTCATCGCGATGTCAAGCCCGATAATGTGCTCTTCGATTCCAGAAACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGTGATAAAGACGGATTTATGTTCGGCGTTGTCGGAACGCCTTATTACGTCGCGCCGGAGGTTTTGAGAGGATCTGAGTACGATCGGAAGGTCGATGTGTGGAGCGCCGGTGTGATTCTTTACACCATGTTGGCCGGATTTCCCCCGTTCTATGGTGACTCTGCGGCGGAGGTCTTTGAGGCTGTTCTTAGAGGAAATCTCAGATTTCCGACTAGGGTCTTTAGGTCGGTTTCTTCGGCGGCTAAAGATCTGATGAAGAAGATGATCTGTCGAGATTTTTCTAGAAGATTCTCAGCTGAACAAGCTCTCAGACACCCATGGATTTTGAGTGGAGGAGAAGGAACATCTTCAGTTGAATAA

Protein sequence

MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPKIMALLPPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRRPFSESEAVIVMKQLLEALAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLRGSEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKKMICRDFSRRFSAEQALRHPWILSGGEGTSSVE
Homology
BLAST of Lsi06G007860 vs. ExPASy Swiss-Prot
Match: Q9SPK4 (Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK1 PE=1 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 4.4e-91
Identity = 159/266 (59.77%), Postives = 202/266 (75.94%), Query Frame = 0

Query: 8   SQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPKIMALLPP 67
           ++YQI EEIGRGRFGT++R + P +  FFACKTI K  L D+ D+ CL+ EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDDYLCLISELC-DSLSLYDRIIRR-PFSESEAVIVMKQLLEALAHCH 127
           HPNI+Q+ D+++ D  L +  EL   S+S+YDR++    F E +     KQ+L+AL+HCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 SHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  +     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 DRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
             KVD+WSAGV+LYTMLAG PPFYG++A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDFSRRFSAEQALRHPWILSGGE 271
           +IC+D SRRFSAEQALRHPWI   GE
Sbjct: 253 LICKDASRRFSAEQALRHPWIQRAGE 278

BLAST of Lsi06G007860 vs. ExPASy Swiss-Prot
Match: Q93VK0 (Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK2 PE=1 SV=2)

HSP 1 Score: 334.0 bits (855), Expect = 1.7e-90
Identity = 161/268 (60.07%), Postives = 204/268 (76.12%), Query Frame = 0

Query: 6   VNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPKIMALL 65
           + + YQ+ +EIGRGRFGTITRCF P + +F+ACKTI K  LID  D+EC+E EP+IMA+L
Sbjct: 7   LENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLIDALDRECIETEPRIMAML 66

Query: 66  PPHPNILQLIDVLENDDYLCLISELCD-SLSLYDRIIRR--PFSESEAVIVMKQLLEALA 125
           PPHPNI+++ D+ E +D L ++ EL D  +++YDR+I      SESE+    KQ+L ALA
Sbjct: 67  PPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSALA 126

Query: 126 HCHSHGVVHRDVKPDNVLFD-SRNNLKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLRG 185
           HCH   VVHRDVKPDNVL D     +KL DFGSA W   +    GVVGTPYYVAPEV+ G
Sbjct: 127 HCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWLGGE-TAEGVVGTPYYVAPEVVMG 186

Query: 186 SEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
            +YD KVD+WSAGV++YTMLAG PPF G++A ++FE++LRGNLRFP + F SVSS AKDL
Sbjct: 187 RKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAKDL 246

Query: 246 MKKMICRDFSRRFSAEQALRHPWILSGG 270
           ++KMICRD SRRFSAE ALRH W+++ G
Sbjct: 247 LRKMICRDVSRRFSAEDALRHSWMMNVG 273

BLAST of Lsi06G007860 vs. ExPASy Swiss-Prot
Match: P28582 (Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2)

HSP 1 Score: 220.3 bits (560), Expect = 2.7e-56
Identity = 108/271 (39.85%), Postives = 175/271 (64.58%), Query Frame = 0

Query: 6   VNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPKIMALL 65
           +  +Y + +E+GRG+FG + +C    S + +ACK+I K  L+ + DKE +++E +I+  L
Sbjct: 77  IRGKYTLGKELGRGQFGCVYQCTENSSGQLYACKSILKRKLVSKNDKEDIKREIQILQHL 136

Query: 66  PPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRR-PFSESEAVIVMKQLLEALAHC 125
              PNI++   V E+   + L+ ELC    L+DRII +  +SE  A  + +Q++  +  C
Sbjct: 137 SGQPNIVEFKGVFEDRQSVHLVMELCAGGELFDRIIAQGHYSERAAATICRQIVNVVHVC 196

Query: 126 HSHGVVHRDVKPDNVLFDSRNN---LKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLRG 185
           H  GV+HRD+KP+N L  S++    LK  DFG + + ++      +VG+ YYVAPEVLR 
Sbjct: 197 HFMGVMHRDLKPENFLLSSKDKDAMLKATDFGLSVFIEEGKVYRNIVGSAYYVAPEVLRR 256

Query: 186 SEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S Y +++D+WSAGVILY +L+G PPF+ ++   +F+A+L G + F +  + SVS++AKDL
Sbjct: 257 S-YGKEIDIWSAGVILYILLSGVPPFWAENEKGIFDAILEGVIDFESEPWPSVSNSAKDL 316

Query: 246 MKKMICRDFSRRFSAEQALRHPWILSGGEGT 273
           ++KM+ +D  RR ++ Q L HPW+  GGE +
Sbjct: 317 VRKMLTQDPRRRITSAQVLDHPWMREGGEAS 346

BLAST of Lsi06G007860 vs. ExPASy Swiss-Prot
Match: P93759 (Calcium-dependent protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CPK14 PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.0e-55
Identity = 115/268 (42.91%), Postives = 171/268 (63.81%), Query Frame = 0

Query: 6   VNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPKIMALL 65
           +  +Y++  E+GRG FG    C    + + FACK+I K  L    D E +++E +IM  +
Sbjct: 50  IKQKYKLGRELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQM 109

Query: 66  PPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRR-PFSESEAVIVMKQLLEALAHC 125
           P HPNI+ L +  E+D  + L+ ELC+   L+DRI+ R  ++E  A  V+K ++E +  C
Sbjct: 110 PEHPNIVTLKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMC 169

Query: 126 HSHGVVHRDVKPDNVLFDSRN---NLKLIDFGSAEWCDKDGFMFG-VVGTPYYVAPEVLR 185
           H HGV+HRD+KP+N LF ++    +LK IDFG + +  K G  F  +VG+PYY+APEVLR
Sbjct: 170 HKHGVMHRDLKPENFLFANKKETASLKAIDFGLSVFF-KPGERFNEIVGSPYYMAPEVLR 229

Query: 186 GSEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 245
            S Y +++D+WSAGVILY +L G PPF+ ++   V +A+L+  + F    +  VS  AKD
Sbjct: 230 RS-YGQEIDIWSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKD 289

Query: 246 LMKKMICRDFSRRFSAEQALRHPWILSG 269
           L+KKM+  D  RR +A+Q L HPWI +G
Sbjct: 290 LIKKMLHPDPRRRLTAQQVLDHPWIQNG 315

BLAST of Lsi06G007860 vs. ExPASy Swiss-Prot
Match: Q9C6P3 (Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 3.0e-55
Identity = 104/267 (38.95%), Postives = 172/267 (64.42%), Query Frame = 0

Query: 10  YQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPKIMALLPPHP 69
           Y + +E+GRG+FG    C    + K FACK+I+K  L+ + DKE + +E +IM  L   P
Sbjct: 73  YTLSKELGRGQFGVTYLCTEKSTGKRFACKSISKKKLVTKGDKEDMRREIQIMQHLSGQP 132

Query: 70  NILQLIDVLENDDYLCLISELCDSLSLYDRIIRR-PFSESEAVIVMKQLLEALAHCHSHG 129
           NI++     E++  + L+ ELC    L+DRI+ +  +SE  A  V +Q++  +  CH  G
Sbjct: 133 NIVEFKGAYEDEKAVNLVMELCAGGELFDRILAKGHYSERAAASVCRQIVNVVNICHFMG 192

Query: 130 VVHRDVKPDNVLFDSRNN---LKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLRGSEYD 189
           V+HRD+KP+N L  S++    +K  DFG + + ++      +VG+ YYVAPEVL+   Y 
Sbjct: 193 VMHRDLKPENFLLSSKDEKALIKATDFGLSVFIEEGRVYKDIVGSAYYVAPEVLK-RRYG 252

Query: 190 RKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKKM 249
           +++D+WSAG+ILY +L+G PPF+ ++   +F+A+L G + F ++ + S+S++AKDL+++M
Sbjct: 253 KEIDIWSAGIILYILLSGVPPFWAETEKGIFDAILEGEIDFESQPWPSISNSAKDLVRRM 312

Query: 250 ICRDFSRRFSAEQALRHPWILSGGEGT 273
           + +D  RR SA + L+HPW+  GGE +
Sbjct: 313 LTQDPKRRISAAEVLKHPWLREGGEAS 338

BLAST of Lsi06G007860 vs. ExPASy TrEMBL
Match: A0A6J1JGI4 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485498 PE=3 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 1.3e-151
Identity = 261/276 (94.57%), Postives = 269/276 (97.46%), Query Frame = 0

Query: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60
           MGDMIVNSQY+ILEEIGRGRFGTITRCFCPISNKFFACKTIAK+DLIDETDKECLEKEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRRPFSESEAVIVMKQLLEA 120
           IMALLPPHPNILQLIDV ENDDYLCLISELCDS+SLYDRIIRRPFSESEA IVMKQLL+A
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCDKDGF FGVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY +KVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFP RVFRS+SSAAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISSAAKD 240

Query: 241 LMKKMICRDFSRRFSAEQALRHPWILSGGEGTSSVE 277
           LMKKMICRD SRRFSAEQALRHPWILSGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEGTSSVE 276

BLAST of Lsi06G007860 vs. ExPASy TrEMBL
Match: A0A6J1HBL3 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461799 PE=3 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 8.7e-151
Identity = 259/276 (93.84%), Postives = 268/276 (97.10%), Query Frame = 0

Query: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60
           MGDMIVNSQY+ILEEIGRGRFGTITRCFCPISNKFFACKTIAK+DLIDETDKECLEKEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRRPFSESEAVIVMKQLLEA 120
           IMALLPPHPNILQLIDV ENDDYLCLISELCDS+SLYDRIIRRPFSESEA IVMKQLL+A
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCDKDGF FGVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY +KVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 240

Query: 241 LMKKMICRDFSRRFSAEQALRHPWILSGGEGTSSVE 277
           LMKKMICRD SRRFSAEQALRHPWI+SGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 276

BLAST of Lsi06G007860 vs. ExPASy TrEMBL
Match: A0A5A7TYV2 (Phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00970 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 3.7e-149
Identity = 258/276 (93.48%), Postives = 266/276 (96.38%), Query Frame = 0

Query: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60
           M DMIVNSQYQILEEIGRGRFG ITRCFCPISNKFFACKTI+K DLIDETDKECL KEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRRPFSESEAVIVMKQLLEA 120
           IMALLPPHPNILQLIDVLEND YLCLIS+LC+ LSLYDRIIRRPFSESEA +VMKQLL+A
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCD+DGFM+GVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY RKVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDFSRRFSAEQALRHPWILSGGEGTSSVE 277
           LMKKMICRD SRR SAEQALRHPWILSGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of Lsi06G007860 vs. ExPASy TrEMBL
Match: A0A1S3CHK9 (phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo OX=3656 GN=LOC103501000 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 3.7e-149
Identity = 258/276 (93.48%), Postives = 266/276 (96.38%), Query Frame = 0

Query: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60
           M DMIVNSQYQILEEIGRGRFG ITRCFCPISNKFFACKTI+K DLIDETDKECL KEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRRPFSESEAVIVMKQLLEA 120
           IMALLPPHPNILQLIDVLEND YLCLIS+LC+ LSLYDRIIRRPFSESEA +VMKQLL+A
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCD+DGFM+GVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY RKVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDFSRRFSAEQALRHPWILSGGEGTSSVE 277
           LMKKMICRD SRR SAEQALRHPWILSGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of Lsi06G007860 vs. ExPASy TrEMBL
Match: A0A0A0M3G7 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G690150 PE=3 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 1.8e-148
Identity = 257/276 (93.12%), Postives = 266/276 (96.38%), Query Frame = 0

Query: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60
           M DMIVNSQYQILEE+GRGRFG ITRCFCPISNKFFACKTI+KNDL DETDKECL KEPK
Sbjct: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRRPFSESEAVIVMKQLLEA 120
           IMALLP HPNILQLIDVLEND YLCLIS+LC+SLSLYDRIIRRPFSESEA +VMKQLL+A
Sbjct: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCD+DGFM+GVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY RKVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDFSRRFSAEQALRHPWILSGGEGTSSVE 277
           LMKKMICRD SRRFSAEQALRHPWILSGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276

BLAST of Lsi06G007860 vs. NCBI nr
Match: XP_038877083.1 (phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida])

HSP 1 Score: 548.1 bits (1411), Expect = 4.3e-152
Identity = 261/274 (95.26%), Postives = 271/274 (98.91%), Query Frame = 0

Query: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60
           MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK
Sbjct: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRRPFSESEAVIVMKQLLEA 120
           IMA+LPPHPNILQLIDVLENDDYLCLIS+LCDSLSLYDRIIRRPFSESEA IV+KQLLEA
Sbjct: 61  IMAILPPHPNILQLIDVLENDDYLCLISDLCDSLSLYDRIIRRPFSESEAAIVIKQLLEA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSA+WCD+DGFMFGVVGTPYY+APEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSADWCDEDGFMFGVVGTPYYIAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY RKVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDFSRRFSAEQALRHPWILSGGEGTSS 275
           LMKKMICRD SRRFSAEQ+LRHPWILSGGEGT++
Sbjct: 241 LMKKMICRDVSRRFSAEQSLRHPWILSGGEGTAT 274

BLAST of Lsi06G007860 vs. NCBI nr
Match: XP_022988181.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita maxima])

HSP 1 Score: 545.4 bits (1404), Expect = 2.8e-151
Identity = 261/276 (94.57%), Postives = 269/276 (97.46%), Query Frame = 0

Query: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60
           MGDMIVNSQY+ILEEIGRGRFGTITRCFCPISNKFFACKTIAK+DLIDETDKECLEKEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRRPFSESEAVIVMKQLLEA 120
           IMALLPPHPNILQLIDV ENDDYLCLISELCDS+SLYDRIIRRPFSESEA IVMKQLL+A
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCDKDGF FGVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY +KVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFP RVFRS+SSAAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISSAAKD 240

Query: 241 LMKKMICRDFSRRFSAEQALRHPWILSGGEGTSSVE 277
           LMKKMICRD SRRFSAEQALRHPWILSGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEGTSSVE 276

BLAST of Lsi06G007860 vs. NCBI nr
Match: KAG7023891.1 (Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 542.7 bits (1397), Expect = 1.8e-150
Identity = 259/276 (93.84%), Postives = 268/276 (97.10%), Query Frame = 0

Query: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60
           MGDMIVNSQY+ILEEIGRGRFGTITRCFCPISNKFFACKTIAK+DLIDETDKECLEKEPK
Sbjct: 43  MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 102

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRRPFSESEAVIVMKQLLEA 120
           IMALLPPHPNILQLIDV ENDDYLCLISELCDS+SLYDRIIRRPFSESEA IVMKQLL+A
Sbjct: 103 IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 162

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCDKDGF FGVVGTPYY+APEVLR
Sbjct: 163 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 222

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY +KVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 223 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 282

Query: 241 LMKKMICRDFSRRFSAEQALRHPWILSGGEGTSSVE 277
           LMKKMICRD SRRFSAEQALRHPWI+SGGEGTSSVE
Sbjct: 283 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 318

BLAST of Lsi06G007860 vs. NCBI nr
Match: XP_022961225.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata] >XP_022961226.1 phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata])

HSP 1 Score: 542.7 bits (1397), Expect = 1.8e-150
Identity = 259/276 (93.84%), Postives = 268/276 (97.10%), Query Frame = 0

Query: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60
           MGDMIVNSQY+ILEEIGRGRFGTITRCFCPISNKFFACKTIAK+DLIDETDKECLEKEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRRPFSESEAVIVMKQLLEA 120
           IMALLPPHPNILQLIDV ENDDYLCLISELCDS+SLYDRIIRRPFSESEA IVMKQLL+A
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCDKDGF FGVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY +KVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 240

Query: 241 LMKKMICRDFSRRFSAEQALRHPWILSGGEGTSSVE 277
           LMKKMICRD SRRFSAEQALRHPWI+SGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 276

BLAST of Lsi06G007860 vs. NCBI nr
Match: KAG6590287.1 (Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 540.8 bits (1392), Expect = 6.8e-150
Identity = 258/276 (93.48%), Postives = 267/276 (96.74%), Query Frame = 0

Query: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60
           MGDMIVNSQY+ILEEIGRGRFGTITRCFCPISNKFFACKTIAK+DLIDETDKECLEKEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRRPFSESEAVIVMKQLLEA 120
           IMALLPPHPNILQLIDV ENDDYLCLISELCDS+SLYDRIIRRPFSESEA IVMKQLL+A
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCDKDGF FGVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY +KVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 240

Query: 241 LMKKMICRDFSRRFSAEQALRHPWILSGGEGTSSVE 277
           LMKKMICRD SRRFSAEQALRHPWI+SGGEGTS VE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSPVE 276

BLAST of Lsi06G007860 vs. TAIR 10
Match: AT1G08650.1 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 335.9 bits (860), Expect = 3.1e-92
Identity = 159/266 (59.77%), Postives = 202/266 (75.94%), Query Frame = 0

Query: 8   SQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPKIMALLPP 67
           ++YQI EEIGRGRFGT++R + P +  FFACKTI K  L D+ D+ CL+ EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDDYLCLISELC-DSLSLYDRIIRR-PFSESEAVIVMKQLLEALAHCH 127
           HPNI+Q+ D+++ D  L +  EL   S+S+YDR++    F E +     KQ+L+AL+HCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 SHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  +     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 DRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
             KVD+WSAGV+LYTMLAG PPFYG++A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDFSRRFSAEQALRHPWILSGGE 271
           +IC+D SRRFSAEQALRHPWI   GE
Sbjct: 253 LICKDASRRFSAEQALRHPWIQRAGE 278

BLAST of Lsi06G007860 vs. TAIR 10
Match: AT3G04530.1 (phosphoenolpyruvate carboxylase kinase 2 )

HSP 1 Score: 334.0 bits (855), Expect = 1.2e-91
Identity = 161/268 (60.07%), Postives = 204/268 (76.12%), Query Frame = 0

Query: 6   VNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPKIMALL 65
           + + YQ+ +EIGRGRFGTITRCF P + +F+ACKTI K  LID  D+EC+E EP+IMA+L
Sbjct: 7   LENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLIDALDRECIETEPRIMAML 66

Query: 66  PPHPNILQLIDVLENDDYLCLISELCD-SLSLYDRIIRR--PFSESEAVIVMKQLLEALA 125
           PPHPNI+++ D+ E +D L ++ EL D  +++YDR+I      SESE+    KQ+L ALA
Sbjct: 67  PPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSALA 126

Query: 126 HCHSHGVVHRDVKPDNVLFD-SRNNLKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLRG 185
           HCH   VVHRDVKPDNVL D     +KL DFGSA W   +    GVVGTPYYVAPEV+ G
Sbjct: 127 HCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWLGGE-TAEGVVGTPYYVAPEVVMG 186

Query: 186 SEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
            +YD KVD+WSAGV++YTMLAG PPF G++A ++FE++LRGNLRFP + F SVSS AKDL
Sbjct: 187 RKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAKDL 246

Query: 246 MKKMICRDFSRRFSAEQALRHPWILSGG 270
           ++KMICRD SRRFSAE ALRH W+++ G
Sbjct: 247 LRKMICRDVSRRFSAEDALRHSWMMNVG 273

BLAST of Lsi06G007860 vs. TAIR 10
Match: AT1G08650.2 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 318.9 bits (816), Expect = 3.9e-87
Identity = 152/256 (59.38%), Postives = 195/256 (76.17%), Query Frame = 0

Query: 8   SQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPKIMALLPP 67
           ++YQI EEIGRGRFGT++R + P +  FFACKTI K  L D+ D+ CL+ EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDDYLCLISELC-DSLSLYDRIIRR-PFSESEAVIVMKQLLEALAHCH 127
           HPNI+Q+ D+++ D  L +  EL   S+S+YDR++    F E +     KQ+L+AL+HCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 SHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  +     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 DRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
             KVD+WSAGV+LYTMLAG PPFYG++A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDFSRRFSAEQAL 261
           +IC+D SRRFSAEQAL
Sbjct: 253 LICKDASRRFSAEQAL 268

BLAST of Lsi06G007860 vs. TAIR 10
Match: AT2G41860.2 (calcium-dependent protein kinase 14 )

HSP 1 Score: 218.8 bits (556), Expect = 5.5e-57
Identity = 112/267 (41.95%), Postives = 168/267 (62.92%), Query Frame = 0

Query: 6   VNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPKIMALL 65
           +  +Y++  E+GRG FG    C    + + FACK+I K  L    D E +++E +IM  +
Sbjct: 50  IKQKYKLGRELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQM 109

Query: 66  PPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRR-PFSESEAVIVMKQLLEALAHC 125
           P HPNI+ L +  E+D  + L+ ELC+   L+DRI+ R  ++E  A  V+K ++E +  C
Sbjct: 110 PEHPNIVTLKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMC 169

Query: 126 HSHGVVHRDVKPDNVLFDSRN---NLKLIDFGSAEWCDKDGFMFGVVGTPYYVAPEVLRG 185
           H HGV+HRD+KP+N LF ++    +LK IDFG + +         +VG+PYY+APEVLR 
Sbjct: 170 HKHGVMHRDLKPENFLFANKKETASLKAIDFGLSVFFKPGDRFNEIVGSPYYMAPEVLRR 229

Query: 186 SEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S Y +++D+WSAGVILY +L G PPF+ ++   V +A+L+  + F    +  VS  AKDL
Sbjct: 230 S-YGQEIDIWSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKDL 289

Query: 246 MKKMICRDFSRRFSAEQALRHPWILSG 269
           +KKM+  D  RR +A+Q L HPWI +G
Sbjct: 290 IKKMLHPDPRRRLTAQQVLDHPWIQNG 315

BLAST of Lsi06G007860 vs. TAIR 10
Match: AT2G41860.1 (calcium-dependent protein kinase 14 )

HSP 1 Score: 218.4 bits (555), Expect = 7.2e-57
Identity = 115/268 (42.91%), Postives = 171/268 (63.81%), Query Frame = 0

Query: 6   VNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPKIMALL 65
           +  +Y++  E+GRG FG    C    + + FACK+I K  L    D E +++E +IM  +
Sbjct: 50  IKQKYKLGRELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQM 109

Query: 66  PPHPNILQLIDVLENDDYLCLISELCDSLSLYDRIIRR-PFSESEAVIVMKQLLEALAHC 125
           P HPNI+ L +  E+D  + L+ ELC+   L+DRI+ R  ++E  A  V+K ++E +  C
Sbjct: 110 PEHPNIVTLKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMC 169

Query: 126 HSHGVVHRDVKPDNVLFDSRN---NLKLIDFGSAEWCDKDGFMFG-VVGTPYYVAPEVLR 185
           H HGV+HRD+KP+N LF ++    +LK IDFG + +  K G  F  +VG+PYY+APEVLR
Sbjct: 170 HKHGVMHRDLKPENFLFANKKETASLKAIDFGLSVFF-KPGERFNEIVGSPYYMAPEVLR 229

Query: 186 GSEYDRKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 245
            S Y +++D+WSAGVILY +L G PPF+ ++   V +A+L+  + F    +  VS  AKD
Sbjct: 230 RS-YGQEIDIWSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKD 289

Query: 246 LMKKMICRDFSRRFSAEQALRHPWILSG 269
           L+KKM+  D  RR +A+Q L HPWI +G
Sbjct: 290 LIKKMLHPDPRRRLTAQQVLDHPWIQNG 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SPK44.4e-9159.77Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
Q93VK01.7e-9060.07Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
P285822.7e-5639.85Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2[more]
P937591.0e-5542.91Calcium-dependent protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CPK14 PE=... [more]
Q9C6P33.0e-5538.95Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1JGI41.3e-15194.57phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1HBL38.7e-15193.84phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A5A7TYV23.7e-14993.48Phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3CHK93.7e-14993.48phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo OX=3656 GN=LOC103501000... [more]
A0A0A0M3G71.8e-14893.12Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G690... [more]
Match NameE-valueIdentityDescription
XP_038877083.14.3e-15295.26phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida][more]
XP_022988181.12.8e-15194.57phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita maxima][more]
KAG7023891.11.8e-15093.84Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp.... [more]
XP_022961225.11.8e-15093.84phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata] >XP_022961226... [more]
KAG6590287.16.8e-15093.48Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp.... [more]
Match NameE-valueIdentityDescription
AT1G08650.13.1e-9259.77phosphoenolpyruvate carboxylase kinase 1 [more]
AT3G04530.11.2e-9160.07phosphoenolpyruvate carboxylase kinase 2 [more]
AT1G08650.23.9e-8759.38phosphoenolpyruvate carboxylase kinase 1 [more]
AT2G41860.25.5e-5741.95calcium-dependent protein kinase 14 [more]
AT2G41860.17.2e-5742.91calcium-dependent protein kinase 14 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 10..265
e-value: 5.3E-91
score: 318.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 10..265
e-value: 5.4E-72
score: 242.3
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 10..265
score: 48.244301
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 4..275
e-value: 3.0E-88
score: 298.2
NoneNo IPR availablePIRSRPIRSR630616-1PIRSR630616-1coord: 8..267
e-value: 2.9E-50
score: 168.9
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 10..223
e-value: 7.0E-7
score: 26.4
NoneNo IPR availablePANTHERPTHR24349:SF410PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE 1coord: 6..274
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 6..274
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 9..264
e-value: 2.93309E-122
score: 347.541
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 129..141
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 16..43
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 5..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G007860.1Lsi06G007860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity