Lsi06G006860 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi06G006860 vs. ExPASy Swiss-Prot
Match: O82251 (Protein TIC 20-II, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-II PE=2 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 2.1e-61
Identity = 116/169 (68.64%), Postives = 138/169 (81.66%), Query Frame = 0

Query: 41  SKPAKSAVIRMSYNPTPATDRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPIL 100
           S+ A +   R SY PTPAT+R+IS  +Y LPFFNSLQYGRFLFAQYP LGL  EP+ PIL
Sbjct: 40  SRRATALTTRASYTPTPATERVISIASYALPFFNSLQYGRFLFAQYPRLGLLFEPIFPIL 99

Query: 101 GLYRSIPHASFVAFFALYLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGR- 160
            LYRS+P+ASFVAFF LYLGVVRN SFS YVRFN+MQAVTLDVLL VP+L+ RIL PG+ 
Sbjct: 100 NLYRSVPYASFVAFFGLYLGVVRNTSFSRYVRFNAMQAVTLDVLLAVPVLLTRILDPGQG 159

Query: 161 SGLGFQIMVWGHNGLFVFSVLCFLYSLVSCLLGRTPHLPLVADAAGRQI 209
            G G + M+WGH G+FVFS +CF+Y +VS LLG+TP++P VADAAGRQ+
Sbjct: 160 GGFGMKAMMWGHTGVFVFSFMCFVYGVVSSLLGKTPYIPFVADAAGRQL 208

BLAST of Lsi06G006860 vs. ExPASy Swiss-Prot
Match: Q9FM67 (Protein TIC 20-v, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-V PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 6.3e-34
Identity = 71/151 (47.02%), Postives = 102/151 (67.55%), Query Frame = 0

Query: 58  ATDRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASFVAFFAL 117
           A+DR+ISAV Y  PFF+ +QYG+F+  QY    + I+PL P +  ++S P   F+ F  L
Sbjct: 59  ASDRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQPLFPAIRAFKSFPFNGFLIFITL 118

Query: 118 YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGRSGLGFQIMVWGHNGLFVF 177
           Y  VVRNP+FS YVRFN+MQA+ LDVLL+ P L++R  +P R G G  +++   + +F+F
Sbjct: 119 YFVVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERSFNP-RDGFGLDVVMSLDSTVFLF 178

Query: 178 SVLCFLYSLVSCLLGRTPHLPLVADAAGRQI 209
            ++  +Y   +CL G TP LPLVA+AA RQ+
Sbjct: 179 LLVSLIYGFSACLFGLTPRLPLVAEAADRQV 208

BLAST of Lsi06G006860 vs. ExPASy Swiss-Prot
Match: P73387 (Tic20 family protein OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1737 PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.7e-21
Identity = 54/151 (35.76%), Postives = 91/151 (60.26%), Query Frame = 0

Query: 60  DRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASFVAFFALYL 119
           DR  SA+ Y +P  ++  +G FL  Q+PVL +   P++P+L  Y   P ASF+ F  L++
Sbjct: 11  DRFFSALIYVIPLIDAFMFGGFLLQQFPVLQIIYLPIMPLLQFYYQFPFASFIIFIVLFM 70

Query: 120 GVVRNPSFSHYVRFNSMQAVTLDVLL-VVPLLVQRILSP-GRSGLGFQIMVWGHNGLFVF 179
            VVRN + SH++RFN+MQA+ + +LL +  L+V  ++ P    GL  + +   +N  F+ 
Sbjct: 71  AVVRNNNISHFIRFNAMQAILIGILLSLFGLIVAYVIQPVFGQGLVTETL---YNFAFLG 130

Query: 180 SVLCFLYSLVSCLLGRTPHLPLVADAAGRQI 209
           ++ C  + +V  +LGR   +P ++DAA  Q+
Sbjct: 131 ALACGFFGIVQSVLGRYAEIPTISDAAYSQV 158

BLAST of Lsi06G006860 vs. ExPASy Swiss-Prot
Match: Q8GZ79 (Protein TIC 20-I, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-I PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 6.2e-05
Identity = 56/224 (25.00%), Postives = 93/224 (41.52%), Query Frame = 0

Query: 3   STSIPLLNLSAKTAI-----KAALVPHSSVIKSRHRSSLPFLPSKPAKSAVIRMSYNPTP 62
           S  +PL  LSA +++     + +L     V+  R ++ L      P  S  +  S+   P
Sbjct: 61  SRGVPLSYLSASSSLLLNGEQGSLSGTLPVLPVRRKTLL-----TPRASKDVPSSFRFPP 120

Query: 63  ATD------RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASF 122
            T       R ++ + Y +P   +  Y    +  +P L        P LG    +P    
Sbjct: 121 MTKKPQWWWRTLACLPYLMPLHETWMYAETAYHLHPFLEDFEFLTYPFLGAIGRLPSWFL 180

Query: 123 VA-FFALYLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGRSGLGFQIMVWG 182
           +A FF  YLG+VR   + H+ RF+ +  + L++ L V   V + +  G        + WG
Sbjct: 181 MAYFFVAYLGIVRRKEWPHFFRFHVVMGMLLEIALQVIGTVSKWMPLG--------VYWG 240

Query: 183 HNGLFVFSVLCFLY------SLVSCLLGRTPHLPLVADAAGRQI 209
             G+  ++ + F Y      S+   L G    +P V DAA  QI
Sbjct: 241 KFGMHFWTAVAFAYLFTVLESIRCALAGMYADIPFVCDAAYIQI 271

BLAST of Lsi06G006860 vs. ExPASy TrEMBL
Match: A0A0A0LXS7 (Protein TIC 20 OS=Cucumis sativus OX=3659 GN=Csa_1G654890 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.2e-98
Identity = 187/208 (89.90%), Postives = 196/208 (94.23%), Query Frame = 0

Query: 1   MASTSIPLLNLSAKTAIKAALVPHSSVIKSRHRSSLPFLPSKPAKSAVIRMSYNPTPATD 60
           MASTSIPLLNLSAKT+I+AAL PHSS IKSRH SSLPFLPSK  K   IRMSYNPTPATD
Sbjct: 1   MASTSIPLLNLSAKTSIRAALFPHSSAIKSRHHSSLPFLPSKSPKFTPIRMSYNPTPATD 60

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYP LG A EPLLPILGLYRSIPH+SFVAFFALYLG
Sbjct: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHSSFVAFFALYLG 120

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGRSGLGFQIMVWGHNGLFVFSVL 180
           VVRNPSF+HYVRFNSMQAVTLDVLLVVPLL+QRILSPGRSG+GF+IMVW HNGLFVFSVL
Sbjct: 121 VVRNPSFNHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRSGIGFKIMVWSHNGLFVFSVL 180

Query: 181 CFLYSLVSCLLGRTPHLPLVADAAGRQI 209
           CFLYS+VSCLLGRTPHLP+VADAAGRQI
Sbjct: 181 CFLYSVVSCLLGRTPHLPIVADAAGRQI 208

BLAST of Lsi06G006860 vs. ExPASy TrEMBL
Match: A0A1S3B9C6 (Protein TIC 20 OS=Cucumis melo OX=3656 GN=LOC103487217 PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 8.4e-98
Identity = 189/211 (89.57%), Postives = 197/211 (93.36%), Query Frame = 0

Query: 1   MASTSIPLLNLSAKTAIKAALVPHSSVIKSRHRSSLPFLPSKPAKSA---VIRMSYNPTP 60
           MASTSIPLLNLSAKTAI+AAL PHSS IKSRH  SLPFLPSK AKSA    IRMSYNPTP
Sbjct: 1   MASTSIPLLNLSAKTAIRAALFPHSSTIKSRHHFSLPFLPSKSAKSAKSTPIRMSYNPTP 60

Query: 61  ATDRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASFVAFFAL 120
           ATDRLISAV+YTLPFFNSLQYGRFLFAQYP LG A EPLLPILGLYRSIPH+SFVAFFAL
Sbjct: 61  ATDRLISAVSYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHSSFVAFFAL 120

Query: 121 YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGRSGLGFQIMVWGHNGLFVF 180
           YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLL+QRILSPGR G+GF+IMVW HNGLFVF
Sbjct: 121 YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFKIMVWSHNGLFVF 180

Query: 181 SVLCFLYSLVSCLLGRTPHLPLVADAAGRQI 209
           SVLCFLYS+VSCLLGRTPHLP+VADAAGRQI
Sbjct: 181 SVLCFLYSMVSCLLGRTPHLPIVADAAGRQI 211

BLAST of Lsi06G006860 vs. ExPASy TrEMBL
Match: A0A5D3E499 (Protein TIC 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84664G00600 PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 8.4e-98
Identity = 189/211 (89.57%), Postives = 197/211 (93.36%), Query Frame = 0

Query: 1   MASTSIPLLNLSAKTAIKAALVPHSSVIKSRHRSSLPFLPSKPAKSA---VIRMSYNPTP 60
           MASTSIPLLNLSAKTAI+AAL PHSS IKSRH  SLPFLPSK AKSA    IRMSYNPTP
Sbjct: 1   MASTSIPLLNLSAKTAIRAALFPHSSTIKSRHHFSLPFLPSKSAKSAKSTPIRMSYNPTP 60

Query: 61  ATDRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASFVAFFAL 120
           ATDRLISAV+YTLPFFNSLQYGRFLFAQYP LG A EPLLPILGLYRSIPH+SFVAFFAL
Sbjct: 61  ATDRLISAVSYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHSSFVAFFAL 120

Query: 121 YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGRSGLGFQIMVWGHNGLFVF 180
           YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLL+QRILSPGR G+GF+IMVW HNGLFVF
Sbjct: 121 YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFKIMVWSHNGLFVF 180

Query: 181 SVLCFLYSLVSCLLGRTPHLPLVADAAGRQI 209
           SVLCFLYS+VSCLLGRTPHLP+VADAAGRQI
Sbjct: 181 SVLCFLYSMVSCLLGRTPHLPIVADAAGRQI 211

BLAST of Lsi06G006860 vs. ExPASy TrEMBL
Match: A0A6J1JJ22 (Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111485044 PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 3.5e-96
Identity = 183/208 (87.98%), Postives = 192/208 (92.31%), Query Frame = 0

Query: 1   MASTSIPLLNLSAKTAIKAALVPHSSVIKSRHRSSLPFLPSKPAKSAVIRMSYNPTPATD 60
           MAS SIPLLNLS K AIKAAL+PH S IKSRH  SLP LPS+PAKS  IRMSYNPTPATD
Sbjct: 3   MASCSIPLLNLSTKPAIKAALIPHYSAIKSRHCFSLPLLPSRPAKSPAIRMSYNPTPATD 62

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLA EPLLPILGLYRSIPH+SF+AFFA YLG
Sbjct: 63  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 122

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGRSGLGFQIMVWGHNGLFVFSVL 180
           VVRNPSFS Y RFNSMQA+TLDVLLVVPLLVQRILSPGRSGLGFQIM W HNGLFVFSVL
Sbjct: 123 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGRSGLGFQIMAWSHNGLFVFSVL 182

Query: 181 CFLYSLVSCLLGRTPHLPLVADAAGRQI 209
           CFLYS+VSC+LGRTPHLP+VADAAGRQ+
Sbjct: 183 CFLYSVVSCILGRTPHLPIVADAAGRQV 210

BLAST of Lsi06G006860 vs. ExPASy TrEMBL
Match: A0A6J1HBN6 (Protein TIC 20 OS=Cucurbita moschata OX=3662 GN=LOC111461378 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 6.0e-96
Identity = 183/208 (87.98%), Postives = 192/208 (92.31%), Query Frame = 0

Query: 1   MASTSIPLLNLSAKTAIKAALVPHSSVIKSRHRSSLPFLPSKPAKSAVIRMSYNPTPATD 60
           MAS SIPLLNLS K AIKAAL+PH S IKSRH  SLP LPSKPAKS  IRMSYNPTPATD
Sbjct: 3   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 62

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLA EPLLPILGLYRSIPH+SF+AFFA YLG
Sbjct: 63  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 122

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGRSGLGFQIMVWGHNGLFVFSVL 180
           VVRNPSFS Y RFNSMQA+TLDVLLVVPLLVQRILSPG+SGLGFQIM W HNGLFVFSVL
Sbjct: 123 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGLFVFSVL 182

Query: 181 CFLYSLVSCLLGRTPHLPLVADAAGRQI 209
           CFLYS+VSC+LGRTPHLP+VADAAGRQ+
Sbjct: 183 CFLYSVVSCILGRTPHLPIVADAAGRQV 210

BLAST of Lsi06G006860 vs. NCBI nr
Match: XP_038879337.1 (protein TIC 20-II, chloroplastic [Benincasa hispida])

HSP 1 Score: 379.4 bits (973), Expect = 2.0e-101
Identity = 191/208 (91.83%), Postives = 199/208 (95.67%), Query Frame = 0

Query: 1   MASTSIPLLNLSAKTAIKAALVPHSSVIKSRHRSSLPFLPSKPAKSAVIRMSYNPTPATD 60
           MAS+SIPLLNLS KTA +AAL+PHSS IKSRHRSSLPFLPSKPAKS V RMSYNPTPATD
Sbjct: 1   MASSSIPLLNLSVKTAFRAALIPHSSAIKSRHRSSLPFLPSKPAKSTVFRMSYNPTPATD 60

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASFVAFFALYLG 120
           R+ISAVAYTLPFFNSLQYGRFLFAQYP LG A EPLLPILGLYRSIPHASFVAFFALYLG
Sbjct: 61  RVISAVAYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHASFVAFFALYLG 120

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGRSGLGFQIMVWGHNGLFVFSVL 180
           +VRNPSFSHYVRFNSMQAVTLDVLLVVPLL+QRILSPGR GLGFQIMVWGHNGLFVFSVL
Sbjct: 121 LVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNGLFVFSVL 180

Query: 181 CFLYSLVSCLLGRTPHLPLVADAAGRQI 209
           CF+YSLVSCLLGRTPHLP+VADAAGRQI
Sbjct: 181 CFVYSLVSCLLGRTPHLPIVADAAGRQI 208

BLAST of Lsi06G006860 vs. NCBI nr
Match: XP_004139099.1 (protein TIC 20-II, chloroplastic [Cucumis sativus] >XP_011660219.1 protein TIC 20-II, chloroplastic [Cucumis sativus] >XP_011660220.1 protein TIC 20-II, chloroplastic [Cucumis sativus] >KGN66660.1 hypothetical protein Csa_006938 [Cucumis sativus])

HSP 1 Score: 368.2 bits (944), Expect = 4.6e-98
Identity = 187/208 (89.90%), Postives = 196/208 (94.23%), Query Frame = 0

Query: 1   MASTSIPLLNLSAKTAIKAALVPHSSVIKSRHRSSLPFLPSKPAKSAVIRMSYNPTPATD 60
           MASTSIPLLNLSAKT+I+AAL PHSS IKSRH SSLPFLPSK  K   IRMSYNPTPATD
Sbjct: 1   MASTSIPLLNLSAKTSIRAALFPHSSAIKSRHHSSLPFLPSKSPKFTPIRMSYNPTPATD 60

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYP LG A EPLLPILGLYRSIPH+SFVAFFALYLG
Sbjct: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHSSFVAFFALYLG 120

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGRSGLGFQIMVWGHNGLFVFSVL 180
           VVRNPSF+HYVRFNSMQAVTLDVLLVVPLL+QRILSPGRSG+GF+IMVW HNGLFVFSVL
Sbjct: 121 VVRNPSFNHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRSGIGFKIMVWSHNGLFVFSVL 180

Query: 181 CFLYSLVSCLLGRTPHLPLVADAAGRQI 209
           CFLYS+VSCLLGRTPHLP+VADAAGRQI
Sbjct: 181 CFLYSVVSCLLGRTPHLPIVADAAGRQI 208

BLAST of Lsi06G006860 vs. NCBI nr
Match: XP_008443678.1 (PREDICTED: protein TIC 20-II, chloroplastic [Cucumis melo] >XP_008443680.1 PREDICTED: protein TIC 20-II, chloroplastic [Cucumis melo] >XP_008443681.1 PREDICTED: protein TIC 20-II, chloroplastic [Cucumis melo] >XP_008443682.1 PREDICTED: protein TIC 20-II, chloroplastic [Cucumis melo] >XP_008443683.1 PREDICTED: protein TIC 20-II, chloroplastic [Cucumis melo] >KAA0038239.1 protein TIC 20-II [Cucumis melo var. makuwa] >TYK30638.1 protein TIC 20-II [Cucumis melo var. makuwa])

HSP 1 Score: 366.3 bits (939), Expect = 1.7e-97
Identity = 189/211 (89.57%), Postives = 197/211 (93.36%), Query Frame = 0

Query: 1   MASTSIPLLNLSAKTAIKAALVPHSSVIKSRHRSSLPFLPSKPAKSA---VIRMSYNPTP 60
           MASTSIPLLNLSAKTAI+AAL PHSS IKSRH  SLPFLPSK AKSA    IRMSYNPTP
Sbjct: 1   MASTSIPLLNLSAKTAIRAALFPHSSTIKSRHHFSLPFLPSKSAKSAKSTPIRMSYNPTP 60

Query: 61  ATDRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASFVAFFAL 120
           ATDRLISAV+YTLPFFNSLQYGRFLFAQYP LG A EPLLPILGLYRSIPH+SFVAFFAL
Sbjct: 61  ATDRLISAVSYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHSSFVAFFAL 120

Query: 121 YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGRSGLGFQIMVWGHNGLFVF 180
           YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLL+QRILSPGR G+GF+IMVW HNGLFVF
Sbjct: 121 YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFKIMVWSHNGLFVF 180

Query: 181 SVLCFLYSLVSCLLGRTPHLPLVADAAGRQI 209
           SVLCFLYS+VSCLLGRTPHLP+VADAAGRQI
Sbjct: 181 SVLCFLYSMVSCLLGRTPHLPIVADAAGRQI 211

BLAST of Lsi06G006860 vs. NCBI nr
Match: XP_022987499.1 (protein TIC 20-II, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 360.9 bits (925), Expect = 7.3e-96
Identity = 183/208 (87.98%), Postives = 192/208 (92.31%), Query Frame = 0

Query: 1   MASTSIPLLNLSAKTAIKAALVPHSSVIKSRHRSSLPFLPSKPAKSAVIRMSYNPTPATD 60
           MAS SIPLLNLS K AIKAAL+PH S IKSRH  SLP LPS+PAKS  IRMSYNPTPATD
Sbjct: 3   MASCSIPLLNLSTKPAIKAALIPHYSAIKSRHCFSLPLLPSRPAKSPAIRMSYNPTPATD 62

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLA EPLLPILGLYRSIPH+SF+AFFA YLG
Sbjct: 63  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 122

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGRSGLGFQIMVWGHNGLFVFSVL 180
           VVRNPSFS Y RFNSMQA+TLDVLLVVPLLVQRILSPGRSGLGFQIM W HNGLFVFSVL
Sbjct: 123 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGRSGLGFQIMAWSHNGLFVFSVL 182

Query: 181 CFLYSLVSCLLGRTPHLPLVADAAGRQI 209
           CFLYS+VSC+LGRTPHLP+VADAAGRQ+
Sbjct: 183 CFLYSVVSCILGRTPHLPIVADAAGRQV 210

BLAST of Lsi06G006860 vs. NCBI nr
Match: XP_022960653.1 (protein TIC 20-II, chloroplastic-like [Cucurbita moschata] >XP_022960654.1 protein TIC 20-II, chloroplastic-like [Cucurbita moschata] >KAG6590106.1 Protein TIC 20-II, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 360.1 bits (923), Expect = 1.2e-95
Identity = 183/208 (87.98%), Postives = 192/208 (92.31%), Query Frame = 0

Query: 1   MASTSIPLLNLSAKTAIKAALVPHSSVIKSRHRSSLPFLPSKPAKSAVIRMSYNPTPATD 60
           MAS SIPLLNLS K AIKAAL+PH S IKSRH  SLP LPSKPAKS  IRMSYNPTPATD
Sbjct: 3   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 62

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLA EPLLPILGLYRSIPH+SF+AFFA YLG
Sbjct: 63  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 122

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGRSGLGFQIMVWGHNGLFVFSVL 180
           VVRNPSFS Y RFNSMQA+TLDVLLVVPLLVQRILSPG+SGLGFQIM W HNGLFVFSVL
Sbjct: 123 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGLFVFSVL 182

Query: 181 CFLYSLVSCLLGRTPHLPLVADAAGRQI 209
           CFLYS+VSC+LGRTPHLP+VADAAGRQ+
Sbjct: 183 CFLYSVVSCILGRTPHLPIVADAAGRQV 210

BLAST of Lsi06G006860 vs. TAIR 10
Match: AT2G47840.1 (Uncharacterised conserved protein ycf60 )

HSP 1 Score: 236.9 bits (603), Expect = 1.5e-62
Identity = 116/169 (68.64%), Postives = 138/169 (81.66%), Query Frame = 0

Query: 41  SKPAKSAVIRMSYNPTPATDRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPIL 100
           S+ A +   R SY PTPAT+R+IS  +Y LPFFNSLQYGRFLFAQYP LGL  EP+ PIL
Sbjct: 40  SRRATALTTRASYTPTPATERVISIASYALPFFNSLQYGRFLFAQYPRLGLLFEPIFPIL 99

Query: 101 GLYRSIPHASFVAFFALYLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGR- 160
            LYRS+P+ASFVAFF LYLGVVRN SFS YVRFN+MQAVTLDVLL VP+L+ RIL PG+ 
Sbjct: 100 NLYRSVPYASFVAFFGLYLGVVRNTSFSRYVRFNAMQAVTLDVLLAVPVLLTRILDPGQG 159

Query: 161 SGLGFQIMVWGHNGLFVFSVLCFLYSLVSCLLGRTPHLPLVADAAGRQI 209
            G G + M+WGH G+FVFS +CF+Y +VS LLG+TP++P VADAAGRQ+
Sbjct: 160 GGFGMKAMMWGHTGVFVFSFMCFVYGVVSSLLGKTPYIPFVADAAGRQL 208

BLAST of Lsi06G006860 vs. TAIR 10
Match: AT5G55710.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast thylakoid membrane, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: Uncharacterised conserved protein ycf60 (TAIR:AT2G47840.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 145.6 bits (366), Expect = 4.5e-35
Identity = 71/151 (47.02%), Postives = 102/151 (67.55%), Query Frame = 0

Query: 58  ATDRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASFVAFFAL 117
           A+DR+ISAV Y  PFF+ +QYG+F+  QY    + I+PL P +  ++S P   F+ F  L
Sbjct: 59  ASDRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQPLFPAIRAFKSFPFNGFLIFITL 118

Query: 118 YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGRSGLGFQIMVWGHNGLFVF 177
           Y  VVRNP+FS YVRFN+MQA+ LDVLL+ P L++R  +P R G G  +++   + +F+F
Sbjct: 119 YFVVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERSFNP-RDGFGLDVVMSLDSTVFLF 178

Query: 178 SVLCFLYSLVSCLLGRTPHLPLVADAAGRQI 209
            ++  +Y   +CL G TP LPLVA+AA RQ+
Sbjct: 179 LLVSLIYGFSACLFGLTPRLPLVAEAADRQV 208

BLAST of Lsi06G006860 vs. TAIR 10
Match: AT1G04940.1 (translocon at the inner envelope membrane of chloroplasts 20 )

HSP 1 Score: 49.3 bits (116), Expect = 4.4e-06
Identity = 56/224 (25.00%), Postives = 93/224 (41.52%), Query Frame = 0

Query: 3   STSIPLLNLSAKTAI-----KAALVPHSSVIKSRHRSSLPFLPSKPAKSAVIRMSYNPTP 62
           S  +PL  LSA +++     + +L     V+  R ++ L      P  S  +  S+   P
Sbjct: 61  SRGVPLSYLSASSSLLLNGEQGSLSGTLPVLPVRRKTLL-----TPRASKDVPSSFRFPP 120

Query: 63  ATD------RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAIEPLLPILGLYRSIPHASF 122
            T       R ++ + Y +P   +  Y    +  +P L        P LG    +P    
Sbjct: 121 MTKKPQWWWRTLACLPYLMPLHETWMYAETAYHLHPFLEDFEFLTYPFLGAIGRLPSWFL 180

Query: 123 VA-FFALYLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLVQRILSPGRSGLGFQIMVWG 182
           +A FF  YLG+VR   + H+ RF+ +  + L++ L V   V + +  G        + WG
Sbjct: 181 MAYFFVAYLGIVRRKEWPHFFRFHVVMGMLLEIALQVIGTVSKWMPLG--------VYWG 240

Query: 183 HNGLFVFSVLCFLY------SLVSCLLGRTPHLPLVADAAGRQI 209
             G+  ++ + F Y      S+   L G    +P V DAA  QI
Sbjct: 241 KFGMHFWTAVAFAYLFTVLESIRCALAGMYADIPFVCDAAYIQI 271