Lsi06G006300 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G006300
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAluminum-activated malate transporter 2-like
Locationchr06: 9154608 .. 9159644 (-)
RNA-Seq ExpressionLsi06G006300
SyntenyLsi06G006300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTGTTTTTATAACCCCAACAACACAATACACACAAGCCTTAGCTTCACTCTAACACAATGCCAACTGAGAAAAACTCTAATGGCTATGAAGGGTTGCTACCTTTATGCTTGAGGTTGGTGAAGCCCGTATTCGCGAAGCTCTTGGCCATGGCGGTCGAGCTAGCAACGATAACAAAGAAGCTAGCGAAAGACGACTCGAGAAGGGTAGTTCACGCTTTGAAAGTTGGATTAGCTATAACTTTGGTGTCTTTGTTCTACTATTTCAAACCTTTATATGATGGATTTGGAACGTCTACAATGTGGGCTATCATCACGGTTATTGTCGTCTTTGAGTTTTCGGTTGGTAAGTGTAATATCGATAAAAAATTTTAATTTAATCTTTTATTCGTATCCAATTAGTGAAGATGATGATTATGAGACCAAGACATTCCGATTTTAATATTTTTAATAAACTATATCATTAGAACTAAAAACTCAAGTTGATAGGTTATGATAAATTTTATATATATTCTATTTATTTTCTTAACGCTCGTGACATGTTTTGAACAGGAGGTACACTAGGGCGAGGTTTAAATAGAGTTATGGCGACGTTTTTAGCTGGAGCTCTAGGGTTTGGAGCTCATTATTTGGCAAGCTTGGGTGGAGAGATTGGCCGACCAATAATGCTTGGCTTGTTTGTGTTCGTACTAGGTAAATTTCTAGTTGCTCATTCCTCGAATGTTTCTTGTAATGAAAAATGAGTAAAGACATGATATAGAAATTAGAAAATTTATGTCAACTTAAATATGTATGTAATTATTTCTAATTGGAATATTATTAGTATACAAAAACAAACGCTGAATATTTCTCTTTTGGGGATTGATATTTGTCATCAAATAGGAATAATAAACCACGTGTAAACTTAAGTTTTACATGCTTATAAACCACTTGCAGTCCAAAGAGCTGTTCAATAAAAAACTGCTACATGACAGTTTTTGGTCTTTTTTTATATAAAAATAAAATACCTTTTTTAAGGAAATTTTTTACAAATAAAAAAAATGTCAAACTATTTACATAAAATAACAAAAAAAAAAAAAAATACTGATAGACATTGATAGACTTCTATCAACATCTATCAGTGATAGACATCTATCATTTCTATTGCTGATAGATCCTGATAGACTTCTATCAGCGTTTATCACAACTATTTAAAAAATTTGCTATTTTGTGTAAATAGTTTATCTTATTTTTCTGTTTTTAAAACTTTTCTATTTTTTAATTTTTTTTTTCTATCTGTATTAAAAAAAGGAGAGTATGAGATTAGGTCATGGTTTTGTTGAAAATATGTTGAAAATGAACTATTTGTGCTGTCGAGGAGGTTACTTTGGGATACGTCAAAAAGTAAAATAGCTACCTCTGAGGAATATAATAAACACAAATATCTAAGTGCTCACCGACCTAATGAGTAAATTTCTAGTATCACAAGAATATATAAAATTTTTTATACTCTCTGTTTATTGAGAACTACGTCAATCTGCTTTTAAAAATAAAGTAGGACTATTAAAATTAATGTGTGATCTATTAATATTTATGAATATAAATTATATATAGTGTAAAAAAGTGAGTATGATACTTTCGTAGTACTAAAAAATTTTCTCCTACCTAACATGTGTTTTGACCAATGATATACAATCAAATAGTCGTTCCACAAGATTAGCCGAAGTTTACATAATTGATGAGAATATTCCAGGGAGTTTAACAAATTTGGGATTATTTTTCATCTAAGTTGTGCTGCCCAAAAACATTATTTATTTGTTTCATTTTTATGTAAATATTATATCGTAAATTAAACTAATATATTTTTCTTTTAGTACAACAAGTGTCGTATGAGAATTTAAACCTCTAATTTTCAGAAAAAAAGTATATGTCAATTACGATTGAGTTATGCCCACTTTGACATTCTAATGCTTAAACCTAAGGAAAAATAATTTTATAATATACATTTTTAAAATAATAAATTATGCACACGATCGTAACTTAACAATTTTGATTGTTTGTTTTCCTTAATAGTCACGATTTTACTTATTTGTTTTTTTTTTATATCTATGAGTATTGAGTGTCAAAAAAATTTGTAGAATATTAAATCTTAAGTAGGTAGCTACTATGGATTAAACTCATTCCCTCTAAGCTCTTTATTAAGTCCAACTTAGACGGTAACTACATCATGATTTAACTAGATATCACCTAACTTGCTAACTTGAACATATAGTTCAACGGGTTAAGACATGTTATCTCTGATCAGAAGATCAAAAGTTGGAATCCTCACCCGTACGTGTCATCAAATCATTTAAAAAAAAAAAACTCACCCAAGTACCACTTAAAAGAAATCTATTTTTTGAAAAGCCCATTTCATGAGTAACAAAAATTATTTTTGTTTTGTTTTTGAAATGAATGGCAGCTGGAATATCAACATTCACAAGATTCTTCCCAAAGATGAAGGCAAGATACGACTATGGGCTGCTGATATTCATATTGACATTCTGTATGGTTTCAGTATCAGGATACAGAGATGATGAAATTGCAAAGCTTGCATATAGTAGGATTTTAACCATTCTCATTGGTTGCTTTTTCACTCTCTTTGTTTGCATTTTTGTCAAACCTGTTTGGGCTGGCACTGATCTTCACTATTTGCTCATCACCAACATTCAATCCCTTGCCATATTCTTTCAAGGTAAAATCTTATTTAACCCTACCATCTCATAATTTTGTTTGATAACTATTTTGAGTTTTCATTTTAGTTCTTAAAAATTAACATTATAAATACTTTTTTTTTCTTCTATACTTGATTTTATTTTAAAATTTTGGCTACAAATTTAAATGTTCATATAGAGAAGGTGCAAATCATGGTAAAAAAAATGGTAAGAAACAAGGCACGGGATTTTTAAAATTAAAAAGAAAAAAACTAAAGTTCAGTTTGAGAATAATCTAGTTTCTTTATTTGTTTTGTTGTCTACATTTTAGGCAGGTTTTCAAAATTTAGCTATGATTTTGTTTCCATTCATGTTTCTTTTTAATTATTGTAGCTAAAATTTGAAAGTAAAAAATATATAGCTTTTAAAGAGGGGAATTTTTCCATATAGAAAAAAGGTTAAACTATTTACAGAAAATAACAAAAAAAAAAAAAAAAAAAAGAAAAAGTGATAGACATTGATAGATGAAATCAATATCTATTAGTGATAAACTTCTATCATTTCTATCATTCATAGACTTTGATAGACTTTTATTAGCATCTATCACAACTATCTAAAAATTTTGCTATTTTGTGTAAATAGTTTTCTTTATTTTTCTATTTTTAAACATTTCTCTTTTAAAAAGGGAAATTATTATCAACAAAAAATATCAAACTATTTATAAATATAGAACACTGATAGACAGTAAAATTTTTCTATATTTGAAAACAACCATTAATTTTTTTTTTTTGTTTTGTTTTTAGAATTTAGTTAAAAATCCAAATTTAATACATTGGTAAACCATGAGTAAAAAATTGTAATTAAAATGAGACCTAAATAGTTACCGAATTGGACTTAAATTGTTGTCTAAATTTGAAGGTGCGGTTTGATAATAATTAATCTAATTTCTTTTAACTTGGTTAAGGAATGTATATTATGAACCATTGGCAGGGTTTGGGGTTGAATATTTTGGATTATCACAAGAAGAAGAGATTTGCAAGGCTGACATGCAAAAATATAGAACTATTCTGAATTCGAAATCGAGTGAAGAATCATTGGTGAGTACTCGAGCTCGATCCAGCTATAAATCTTTTAGGACTTGTTTGATAATCTTCTCATTCCTGCATATATATATATATATATATATATATATATATATTTGGGCAGAGTAACCTTGCAAGATGGGAGCCTCGGCATGGGAAGTTCAGATATCGGCATCCTTGGAACCAATATCTGGAAATTGGAAGCTTAAATAGGGACTGTGCCTATCGCCTCGAACTTCTTAATGGATACCTCAAACCCAACCAATTTCAAGTAAGATTCAATGCAAATTTTGATCCTCGACTTTTAGAATTGTATCGATTGGATTTCTACGTACTTTAAAAAGTTTATAATAGATCTATAACTAATTTCAATTTTGCATGTAATAAATCTTTTCAACTCCGCTAGTTTATATATGTTATATGCTTACTACTCTATTATTTACATATGCTTACTACTTTATTATTTAATGATTAGTAAAAACTATTTTTTTCTTCTAGTTTTTAAAACTTGTTCGGATAAGGTAGGTAACAAAATAATAAAGAAATTTAGAAGTGAAAGTGAAGATAATTAAGCTTAATTTTCAAAACAACAAATATAAAAAAACCAAATAGCTATCAAACTCAAAGTACTTTATTTTTTATTTATTTATTTTAGAATTTGGTTAAGAATACAAATACATCTTAATAGAGAAGAAAACCATGGCAAATAAACATGGTGAGAAAACAAGTACTAAGTTTTCAAAAACAAAAAACCAAATAATTATAAAAAACAAAAATCTAATGAAAAACCCTATATATGCTGATGATCTTTTCATTTGCAGATGCCATCGCAACAAATCCATGGTCAATTTCAAGAGGAATGCATGAAGATATGCAGTGAATCAAGTAGTGGATTAAGAGAATTAGCATTATCACTAAGAAAAATGGTTCCTCCACTAACAGCCAAATCCCACATAGAGAAAGCAAAGATTGCAGCTGAGAATCTCAAGACCCATTTGGAAGAATGGAGATTTGAAGAAGTGAATAATGCTATGGAGATTGTTCATATTGTTTCTTTGGCATCACTTCTATTTGATACAATTTGCTGCATAGAGAAGATTGTGGATTCAGTTCAAAAGTTGGCTTCAATGGCTGGTTTCAAGGCTGTGGAAATGCAATCAAGTGTAGCACCACAGCAACAAAAAGATTTGCAAGATGAAGATTCATTGCAGCCATGCCATGGAACTACTATCCTTTTAGCTCATCATGTCATTACAGTTGATGAAGGAGTTCCTTGTTAA

mRNA sequence

ATGGCATTGTTGGTGAAGCCCGTATTCGCGAAGCTCTTGGCCATGGCGGTCGAGCTAGCAACGATAACAAAGAAGCTAGCGAAAGACGACTCGAGAAGGGTAGTTCACGCTTTGAAAGTTGGATTAGCTATAACTTTGGTGTCTTTGTTCTACTATTTCAAACCTTTATATGATGGATTTGGAACGTCTACAATGTGGGCTATCATCACGGTTATTGTCGTCTTTGAGTTTTCGGTTGGAGGTACACTAGGGCGAGGTTTAAATAGAGTTATGGCGACGTTTTTAGCTGGAGCTCTAGGGTTTGGAGCTCATTATTTGGCAAGCTTGGGTGGAGAGATTGGCCGACCAATAATGCTTGGCTTGTTTGTGTTCGTACTAGCTGGAATATCAACATTCACAAGATTCTTCCCAAAGATGAAGGCAAGATACGACTATGGGCTGCTGATATTCATATTGACATTCTGTATGGTTTCAGTATCAGGATACAGAGATGATGAAATTGCAAAGCTTGCATATAGTAGGATTTTAACCATTCTCATTGGTTGCTTTTTCACTCTCTTTGTTTGCATTTTTGTCAAACCTGTTTGGGCTGGCACTGATCTTCACTATTTGCTCATCACCAACATTCAATCCCTTGCCATATTCTTTCAAGGGTTTGGGGTTGAATATTTTGGATTATCACAAGAAGAAGAGATTTGCAAGGCTGACATGCAAAAATATAGAACTATTCTGAATTCGAAATCGAGTGAAGAATCATTGAGTAACCTTGCAAGATGGGAGCCTCGGCATGGGAAGTTCAGATATCGGCATCCTTGGAACCAATATCTGGAAATTGGAAGCTTAAATAGGGACTGTGCCTATCGCCTCGAACTTCTTAATGGATACCTCAAACCCAACCAATTTCAAGTAAGATTCAATGCAAATTTTGATCCTCGACTTTTAGAATTGTATCGATTGGATTTCTACATATGCAGTGAATCAAGTAGTGGATTAAGAGAATTAGCATTATCACTAAGAAAAATGGTTCCTCCACTAACAGCCAAATCCCACATAGAGAAAGCAAAGATTGCAGCTGAGAATCTCAAGACCCATTTGGAAGAATGGAGATTTGAAGAAGTGAATAATGCTATGGAGATTGTTCATATTGTTTCTTTGGCATCACTTCTATTTGATACAATTTGCTGCATAGAGAAGATTGTGGATTCAGTTCAAAAGTTGGCTTCAATGGCTGGTTTCAAGGCTGTGGAAATGCAATCAAGTGTAGCACCACAGCAACAAAAAGATTTGCAAGATGAAGATTCATTGCAGCCATGCCATGGAACTACTATCCTTTTAGCTCATCATGTCATTACAGTTGATGAAGGAGTTCCTTGTTAA

Coding sequence (CDS)

ATGGCATTGTTGGTGAAGCCCGTATTCGCGAAGCTCTTGGCCATGGCGGTCGAGCTAGCAACGATAACAAAGAAGCTAGCGAAAGACGACTCGAGAAGGGTAGTTCACGCTTTGAAAGTTGGATTAGCTATAACTTTGGTGTCTTTGTTCTACTATTTCAAACCTTTATATGATGGATTTGGAACGTCTACAATGTGGGCTATCATCACGGTTATTGTCGTCTTTGAGTTTTCGGTTGGAGGTACACTAGGGCGAGGTTTAAATAGAGTTATGGCGACGTTTTTAGCTGGAGCTCTAGGGTTTGGAGCTCATTATTTGGCAAGCTTGGGTGGAGAGATTGGCCGACCAATAATGCTTGGCTTGTTTGTGTTCGTACTAGCTGGAATATCAACATTCACAAGATTCTTCCCAAAGATGAAGGCAAGATACGACTATGGGCTGCTGATATTCATATTGACATTCTGTATGGTTTCAGTATCAGGATACAGAGATGATGAAATTGCAAAGCTTGCATATAGTAGGATTTTAACCATTCTCATTGGTTGCTTTTTCACTCTCTTTGTTTGCATTTTTGTCAAACCTGTTTGGGCTGGCACTGATCTTCACTATTTGCTCATCACCAACATTCAATCCCTTGCCATATTCTTTCAAGGGTTTGGGGTTGAATATTTTGGATTATCACAAGAAGAAGAGATTTGCAAGGCTGACATGCAAAAATATAGAACTATTCTGAATTCGAAATCGAGTGAAGAATCATTGAGTAACCTTGCAAGATGGGAGCCTCGGCATGGGAAGTTCAGATATCGGCATCCTTGGAACCAATATCTGGAAATTGGAAGCTTAAATAGGGACTGTGCCTATCGCCTCGAACTTCTTAATGGATACCTCAAACCCAACCAATTTCAAGTAAGATTCAATGCAAATTTTGATCCTCGACTTTTAGAATTGTATCGATTGGATTTCTACATATGCAGTGAATCAAGTAGTGGATTAAGAGAATTAGCATTATCACTAAGAAAAATGGTTCCTCCACTAACAGCCAAATCCCACATAGAGAAAGCAAAGATTGCAGCTGAGAATCTCAAGACCCATTTGGAAGAATGGAGATTTGAAGAAGTGAATAATGCTATGGAGATTGTTCATATTGTTTCTTTGGCATCACTTCTATTTGATACAATTTGCTGCATAGAGAAGATTGTGGATTCAGTTCAAAAGTTGGCTTCAATGGCTGGTTTCAAGGCTGTGGAAATGCAATCAAGTGTAGCACCACAGCAACAAAAAGATTTGCAAGATGAAGATTCATTGCAGCCATGCCATGGAACTACTATCCTTTTAGCTCATCATGTCATTACAGTTGATGAAGGAGTTCCTTGTTAA

Protein sequence

MALLVKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTSTMWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTILNSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVRFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTHLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVAPQQQKDLQDEDSLQPCHGTTILLAHHVITVDEGVPC
Homology
BLAST of Lsi06G006300 vs. ExPASy Swiss-Prot
Match: Q9SJE8 (Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2 PE=2 SV=2)

HSP 1 Score: 367.9 bits (943), Expect = 1.7e-100
Identity = 198/437 (45.31%), Postives = 290/437 (66.36%), Query Frame = 0

Query: 18  ELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTSTMWAIITVIVVFEF 77
           E+    +++ K+D RRVVHA KVGLA+ LVS FYY++PLYD FG + MWA++TV+VVFEF
Sbjct: 6   EIVREGRRVGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVVFEF 65

Query: 78  SVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFP 137
           SVG TLG+GLNR +AT +AG LG GAH+LASL G    PI+L +FVFVLA +STF RFFP
Sbjct: 66  SVGATLGKGLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVRFFP 125

Query: 138 KMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWA 197
           ++KARYDYG+LIFILTF ++SVSG+R+DEI  LA+ R+ T+++G    + + IFV PVWA
Sbjct: 126 RVKARYDYGVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCPVWA 185

Query: 198 GTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKAD-----MQKYRTILNSKSSEES 257
           G DLH LL +N  +L+ F Q FG EYF  +++ +I + +     +++Y+++LNSKS+EE+
Sbjct: 186 GQDLHSLLASNFDTLSHFLQEFGDEYFEATEDGDIKEVEKRRRNLERYKSVLNSKSNEEA 245

Query: 258 LSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVRFNANFDPR 317
           L+N A+WEPRHG+FR+RHPW QYL +G+L R  AYR++ LN  +  +   ++   +   +
Sbjct: 246 LANFAKWEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSD---MQIPMDIKKK 305

Query: 318 LLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTHLEEWRFEE 377
           + E  R    + SES   ++E+++SL+ M    +   H+  ++ A + L T L+     +
Sbjct: 306 IEEPLR---RMSSESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKSGILND 365

Query: 378 VNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQS-----SVAPQQQK 437
           V   ++++ +++  SLL D +   EKI +SV +LAS A FK  +  S     S+      
Sbjct: 366 V-EPLQMISLMTTVSLLIDIVNLTEKISESVHELASAAKFKNKKKPSKSNSGSIGQAMPN 425

Query: 438 DLQDEDSLQPCHGTTIL 445
              D+D     H  TIL
Sbjct: 426 KSHDDDD----HVVTIL 431

BLAST of Lsi06G006300 vs. ExPASy Swiss-Prot
Match: Q9XIN1 (Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7 PE=3 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.1e-99
Identity = 196/428 (45.79%), Postives = 272/428 (63.55%), Query Frame = 0

Query: 18  ELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTSTMWAIITVIVVFEF 77
           E+    +++AK+D RRVVH+ KVGL + LVS FYY++PLYD FG + MWA++TV+VVFEF
Sbjct: 6   EIVREGRRVAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVVFEF 65

Query: 78  SVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFP 137
           SVG TLG+GLNRV AT  AG LG GAH+LAS+ G  G PI+L +FVFV A +STF RFFP
Sbjct: 66  SVGATLGKGLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVRFFP 125

Query: 138 KMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWA 197
           ++KARYDY LLIFILTF ++SVSG+R++++ KL + RI T++IG    + + IFV PVWA
Sbjct: 126 RVKARYDYSLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCPVWA 185

Query: 198 GTDLHYLLITNIQSLAIFFQG--------------------------FGVEYFGL----- 257
           G DLH L+ +N + L+ F  G                          FG +Y  +     
Sbjct: 186 GQDLHSLIASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVVENDG 245

Query: 258 SQEEEICKADMQKYRTILNSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCA 317
           ++E +  K D   Y+++LNSKS+EESL+N A+WEP HG+FR+RHPW QYL +G L R CA
Sbjct: 246 AKEVDKRKKDFDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELIRQCA 305

Query: 318 YRLELLNGYLK-PNQFQVRFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPL 377
           YR+  LN YL   NQ  V         L  +         ES   ++E+++SL+KM  P 
Sbjct: 306 YRIHALNSYLNADNQVSVDIKKKLGEPLRRM-------SLESGKAMKEMSISLKKMTKPS 365

Query: 378 TAKSHIEKAKIAAENLKTHLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQK 414
           ++  H++ AK A ++L   L     +EV   +E+V +++  SLL D I   EKI++S+ +
Sbjct: 366 SSDLHVQNAKSACKSLTNLLNSGILKEV-EPLELVSLLTAISLLIDIINLTEKILESLHE 425

BLAST of Lsi06G006300 vs. ExPASy Swiss-Prot
Match: Q9SRM9 (Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8 PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.4e-96
Identity = 187/391 (47.83%), Postives = 263/391 (67.26%), Query Frame = 0

Query: 24  KKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTSTMWAIITVIVVFEFSVGGTL 83
           +K AKDD RR++H++KVG+A+TLVSL YY +PLY  FG + MWAI+TV+VVFEF+VGGTL
Sbjct: 35  QKFAKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAILTVVVVFEFTVGGTL 94

Query: 84  GRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFPKMKARY 143
            +GLNR  AT +AGALG GA +LA   G  G PI+LG+ VF L   +TF+RFFP++K RY
Sbjct: 95  SKGLNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAAATFSRFFPRIKQRY 154

Query: 144 DYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWAGTDLHY 203
           DYG LIFILTF  V++SGYR DEI  +AY R+ TILIG    + V IF+ PVWAG DLH 
Sbjct: 155 DYGALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVSIFICPVWAGEDLHK 214

Query: 204 LLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTILNSKSSEESLSNLARWEPRH 263
           ++  NI  LA + +GF  EYF   +  +   + +++Y++IL SKS+E+SL+NLARWEP H
Sbjct: 215 MIANNINKLAKYLEGFEGEYFQPEKISKETSSCVREYKSILTSKSTEDSLANLARWEPGH 274

Query: 264 GKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVRFNANFDPRLLELYRLDFYI 323
           G+FR RHPW +YL+I  L R CA  LE+LNGY+  N    +    F+ ++ E       +
Sbjct: 275 GRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSND---KAPQEFESKIQEPITT---M 334

Query: 324 CSESSSGLRELALSLRKM-VPPLTAKSHIEKAKIAAENLKTHLEEWRFEEVNNAMEIVHI 383
             E    L+ +A S++ M        +HI+ +K A +NLK  L+    E   + +EI+  
Sbjct: 335 SREVGEALKAIAKSIKTMRNDSACVNAHIDNSKKAIKNLKIALKSSYPETYKDLLEIIPG 394

Query: 384 VSLASLLFDTICCIEKIVDSVQKLASMAGFK 414
           V++AS+L + + C+EKI ++V++ + +A FK
Sbjct: 395 VTMASILIEVVNCVEKIYEAVEEFSGLAHFK 419

BLAST of Lsi06G006300 vs. ExPASy Swiss-Prot
Match: Q9SJE9 (Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.6e-93
Identity = 189/437 (43.25%), Postives = 278/437 (63.62%), Query Frame = 0

Query: 25  KLAKDDSRRVVHALKVGLAITLVSLFYY---FKPLYDGFGTSTMWAIITVIVVFEFSVGG 84
           ++  +D RR++HA KVGLA+ LVS FYY   F P  D FG + MWA++TV+VVFEFSVG 
Sbjct: 13  RVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTVVVVFEFSVGA 72

Query: 85  TLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFPKMKA 144
           TLG+GLNR +AT +AG LG GAH LA L G    PI+L + VFV A +STF RFFP +K 
Sbjct: 73  TLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALSTFVRFFPWVKT 132

Query: 145 RYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWAGTDL 204
           ++DYG+LIFILTF ++S+SG+RD+EI  LA SR+ T++IG    + + IFV PVWAG DL
Sbjct: 133 KFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIFVCPVWAGQDL 192

Query: 205 HYLLITNIQSLAIFFQGFGVEYF-----GLSQEEEICKADMQKYRTILNSKSSEESLSNL 264
           H LL +N  +L+ F Q FG EYF     G  +  E  K ++++Y+++L+SKS EE+L+N 
Sbjct: 193 HSLLASNFDTLSHFLQDFGDEYFEAREKGDYKVVEKRKKNLERYKSVLDSKSDEEALANY 252

Query: 265 ARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPN-QFQVRFNANFDPRLLE 324
           A WEP HG+FR+RHPW QY+ +G+L R CAYR++ LN Y+  + Q  V      +  L  
Sbjct: 253 AEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDIKKKLETPLRR 312

Query: 325 LYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTHLEEWRFEEVNN 384
           +        SES + ++E+++SL++M+   ++  H+  ++ A ++L T L+     +V  
Sbjct: 313 M-------SSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKSGILNDV-E 372

Query: 385 AMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVAPQQQKDLQDEDSL 444
            ++++ +++  S+L D +   EKI +SV +LAS A FK     + + P    +  D  S+
Sbjct: 373 PLQMISLMTTVSMLIDIVNLTEKISESVHELASAARFK-----NKMRPTVLYEKSDSGSI 432

Query: 445 QPCHGTTILLAHHVITV 453
                      HHV+TV
Sbjct: 433 GRAMPIDSHEDHHVVTV 436

BLAST of Lsi06G006300 vs. ExPASy Swiss-Prot
Match: Q76LB1 (Aluminum-activated malate transporter 1 OS=Triticum aestivum OX=4565 GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 4.1e-78
Identity = 175/429 (40.79%), Postives = 260/429 (60.61%), Query Frame = 0

Query: 3   LLVKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGT 62
           LL+  + A L   A   A      A++D RRV H+LKVGLA+ LVS+ Y+  PL++G G 
Sbjct: 22  LLLHSLLAGLGRRAAGFARKVGGAAREDPRRVAHSLKVGLALALVSVVYFVTPLFNGLGV 81

Query: 63  STMWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLG---GEIGRPIML 122
           S +WA++TV+VV E++VG TL +GLNR +AT +AG +  GAH LA L    G+ G PI+L
Sbjct: 82  SAIWAVLTVVVVMEYTVGATLSKGLNRALATLVAGCIAVGAHQLAELAERCGDQGEPIVL 141

Query: 123 GLFVFVLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTIL 182
            + VF +A  +TF RF P++KA+YDYG+ IFILTF +V+VS YR +E+ +LA+ R  TI 
Sbjct: 142 TVLVFFVASAATFLRFIPEIKAKYDYGVTIFILTFGLVAVSSYRVEELIQLAHQRFYTIA 201

Query: 183 IGCFFTLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEIC-----K 242
           +G F  L   +F+ PVWAG D+H L   N+  LA F +G     FG   E  +      K
Sbjct: 202 VGVFICLCTTVFLFPVWAGEDVHKLASGNLDKLAQFIEGMEFNCFG---ENSVANNFGGK 261

Query: 243 ADMQKYRTILNSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNG 302
              Q ++++LNSK++E+SL   A+WEPRHG+FR+RHPW+QY ++G+L R CA  +E L  
Sbjct: 262 DSPQMHKSVLNSKATEDSLCTFAKWEPRHGQFRFRHPWSQYQKLGTLCRQCASSMEALAS 321

Query: 303 YLKPNQFQVRFNANFDPRL-LELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEK 362
           Y+     + +  A  +P L  ++ +    +   SS  LR+LA++ R M  P      +  
Sbjct: 322 YVITTS-KTQCPAAANPELSCKVRKTCGEMSLHSSKVLRDLAMATRTMTVPSPVNITMAT 381

Query: 363 AKIAAENLKTHLEEWRFEEVNNA-MEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGF 422
           A  AAE+L++ L E      N A ++++H+   A+LL D +  +++I + V  LA +A F
Sbjct: 382 AVKAAESLRSELAE------NTALLQVMHVAVTATLLADLVDRVKEIAECVDVLARLAHF 440

BLAST of Lsi06G006300 vs. ExPASy TrEMBL
Match: A0A5A7UR04 (Aluminum-activated malate transporter 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold319G00250 PE=3 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 1.8e-201
Identity = 369/456 (80.92%), Postives = 403/456 (88.38%), Query Frame = 0

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +KP+FAKL   AV+LAT+TKKLAKDDSRRVVHA KVGLAI+LVSL YYFK LYDGFGTST
Sbjct: 21  LKPIFAKLSNKAVKLATMTKKLAKDDSRRVVHAFKVGLAISLVSLLYYFKALYDGFGTST 80

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWAI+TVIVV EFSVGGTLGRGLNRVMAT LAG LGFGAHYLASLGG+ GRPI+L  FVF
Sbjct: 81  MWAIVTVIVVVEFSVGGTLGRGLNRVMATLLAGGLGFGAHYLASLGGDTGRPIILAFFVF 140

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           +LA ISTFTRFFPK+KARYDYG+LIFILTFCMVS+SGYRDDEIAKLA SRILTILIGC F
Sbjct: 141 LLASISTFTRFFPKIKARYDYGMLIFILTFCMVSLSGYRDDEIAKLALSRILTILIGCCF 200

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
           TLFVCIFV+PVWAGTDLH L+  NIQSLA+FFQGFG E+F LSQ EE+ K DMQKYRTIL
Sbjct: 201 TLFVCIFVRPVWAGTDLHCLVANNIQSLALFFQGFGAEFFRLSQ-EEVSKDDMQKYRTIL 260

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVR 304
           NSKS+EESL+NLARWEPRHGKFRYRHPW QYL+IGSLNR+CAYRLELLNGYLK NQFQ+ 
Sbjct: 261 NSKSNEESLTNLARWEPRHGKFRYRHPWKQYLKIGSLNRECAYRLELLNGYLKTNQFQMP 320

Query: 305 FNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTH 364
                 P   E  +    ICSESS GLRELAL+LRKMV PLTAKSHIEKAK+AAE+LK+H
Sbjct: 321 SQQIHGPFKEECMK----ICSESSRGLRELALALRKMVLPLTAKSHIEKAKVAAEDLKSH 380

Query: 365 LEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVAPQ 424
           LEEWRFEEVNNAMEIV +VSLASLLFDTICCIEKIVDSVQ+LASMAGFKAVE+QSSVAP+
Sbjct: 381 LEEWRFEEVNNAMEIVQVVSLASLLFDTICCIEKIVDSVQELASMAGFKAVEVQSSVAPE 440

Query: 425 QQKDLQDED--SLQPCHGTTILLAHHVITVDEGVPC 459
           QQ DLQD+D  SLQP HG  +LLAHH IT+DE  PC
Sbjct: 441 QQMDLQDQDQYSLQPSHGAAVLLAHHAITIDEQSPC 471

BLAST of Lsi06G006300 vs. ExPASy TrEMBL
Match: A0A0A0M301 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G630850 PE=3 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 5.2e-201
Identity = 366/460 (79.57%), Postives = 405/460 (88.04%), Query Frame = 0

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +KP+FAKL  ++V+LAT+ KKLAKDDSRRVVHALKVGLAI+LVSL YYFKPLYDGFGTST
Sbjct: 21  LKPIFAKLSTVSVKLATMAKKLAKDDSRRVVHALKVGLAISLVSLLYYFKPLYDGFGTST 80

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWAI+TVIVVFEFSVGGTLGRGLNRVMAT LAG LGFG HYLASLGG+ GRPI+L LFVF
Sbjct: 81  MWAIVTVIVVFEFSVGGTLGRGLNRVMATLLAGGLGFGTHYLASLGGDTGRPIILALFVF 140

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           +LA +STFTRFFPK+KARYDYGLLI ILTFCMVS+SGYRD+EIAKLA SRILTILIGC  
Sbjct: 141 ILASVSTFTRFFPKIKARYDYGLLILILTFCMVSLSGYRDEEIAKLALSRILTILIGCCV 200

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
           TL VCIFV+PVWAGTDLH L+  NIQSLA+FFQGFG E+FGLSQE E+   DMQKYRTIL
Sbjct: 201 TLIVCIFVRPVWAGTDLHCLVANNIQSLALFFQGFGAEFFGLSQEGEVSNDDMQKYRTIL 260

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQV- 304
           NSKS+EESL+NLARWEPRHGKFRYRHPW QYL+IGSLNR+CAYRLELLNGYLK NQFQ+ 
Sbjct: 261 NSKSNEESLTNLARWEPRHGKFRYRHPWKQYLKIGSLNRECAYRLELLNGYLKTNQFQMP 320

Query: 305 --RFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENL 364
             + +  F    ++       ICSESS GLRELAL+LRKMV PLTAKSHIEKAKIAAENL
Sbjct: 321 SQQIHGQFKEECMK-------ICSESSRGLRELALALRKMVLPLTAKSHIEKAKIAAENL 380

Query: 365 KTHLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSV 424
           K+HLEEWRFEEVNNAMEIV +VSLASLLFDTICCIEKIVDSVQ+LASMAGFKAVE+QSSV
Sbjct: 381 KSHLEEWRFEEVNNAMEIVQVVSLASLLFDTICCIEKIVDSVQELASMAGFKAVEVQSSV 440

Query: 425 APQQQKDLQDED--SLQP-CHGTTILLAHHVITVDEGVPC 459
           AP+QQ DLQD+D  +LQP  HG  +LLAHH IT+DE  PC
Sbjct: 441 APEQQMDLQDQDQYALQPLSHGAAVLLAHHAITIDEQSPC 473

BLAST of Lsi06G006300 vs. ExPASy TrEMBL
Match: A0A1S3BNX0 (aluminum-activated malate transporter 2-like OS=Cucumis melo OX=3656 GN=LOC103491947 PE=3 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 8.9e-201
Identity = 368/456 (80.70%), Postives = 402/456 (88.16%), Query Frame = 0

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +KP+FAKL   AV+LAT+TKKLAKDDSRRVVHA KVGLAI+LVSL YYFK LYDGFGTST
Sbjct: 21  LKPIFAKLSNKAVKLATMTKKLAKDDSRRVVHAFKVGLAISLVSLLYYFKALYDGFGTST 80

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWAI+TVIVV EFSVGGTLGRGLNRVMAT LAG LGFGAHYLASL G+ GRPI+L  FVF
Sbjct: 81  MWAIVTVIVVVEFSVGGTLGRGLNRVMATLLAGGLGFGAHYLASLSGDTGRPIILAFFVF 140

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           +LA ISTFTRFFPK+KARYDYG+LIFILTFCMVS+SGYRDDEIAKLA SRILTILIGC F
Sbjct: 141 LLASISTFTRFFPKIKARYDYGMLIFILTFCMVSLSGYRDDEIAKLALSRILTILIGCCF 200

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
           TLFVCIFV+PVWAGTDLH L+  NIQSLA+FFQGFG E+F LSQ EE+ K DMQKYRTIL
Sbjct: 201 TLFVCIFVRPVWAGTDLHCLVANNIQSLALFFQGFGAEFFRLSQ-EEVSKDDMQKYRTIL 260

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVR 304
           NSKS+EESL+NLARWEPRHGKFRYRHPW QYL+IGSLNR+CAYRLELLNGYLK NQFQ+ 
Sbjct: 261 NSKSNEESLTNLARWEPRHGKFRYRHPWKQYLKIGSLNRECAYRLELLNGYLKTNQFQMP 320

Query: 305 FNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTH 364
                 P   E  +    ICSESS GLRELAL+LRKMV PLTAKSHIEKAK+AAE+LK+H
Sbjct: 321 SQQIHGPFKEECMK----ICSESSRGLRELALALRKMVLPLTAKSHIEKAKVAAEDLKSH 380

Query: 365 LEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVAPQ 424
           LEEWRFEEVNNAMEIV +VSLASLLFDTICCIEKIVDSVQ+LASMAGFKAVE+QSSVAP+
Sbjct: 381 LEEWRFEEVNNAMEIVQVVSLASLLFDTICCIEKIVDSVQELASMAGFKAVEVQSSVAPE 440

Query: 425 QQKDLQDED--SLQPCHGTTILLAHHVITVDEGVPC 459
           QQ DLQD+D  SLQP HG  +LLAHH IT+DE  PC
Sbjct: 441 QQMDLQDQDQYSLQPSHGAAVLLAHHAITIDEQSPC 471

BLAST of Lsi06G006300 vs. ExPASy TrEMBL
Match: A0A6J1H7Y1 (aluminum-activated malate transporter 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461353 PE=3 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 9.5e-195
Identity = 359/458 (78.38%), Postives = 398/458 (86.90%), Query Frame = 0

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           VKP+FAKL A+AVELAT+T KLAKDD+RRVVHALKVGLAITLVSLFYYFKPLYDGFGTST
Sbjct: 21  VKPIFAKLPAIAVELATMTTKLAKDDTRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 80

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWA++TVIVVFEFSVGGTLGRGLNRVMAT LAGALGFGAHYLASLGG+ GRPIMLGLFVF
Sbjct: 81  MWAVVTVIVVFEFSVGGTLGRGLNRVMATLLAGALGFGAHYLASLGGDTGRPIMLGLFVF 140

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           VLA ISTF RFFPKMKARYDYGLLIFILTFCMVS+SGYRDDEIAKLAYSR+LTILIGC F
Sbjct: 141 VLAAISTFIRFFPKMKARYDYGLLIFILTFCMVSLSGYRDDEIAKLAYSRMLTILIGCCF 200

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEE-EICKADMQKYRTI 244
           T+FVC+FV PVWAGTDLH+L+  NI+SLA FF GFG EYFGL QE+ E+ K DMQKYRTI
Sbjct: 201 TVFVCVFVCPVWAGTDLHHLVANNIESLATFFHGFGAEYFGLLQEQGEVGKVDMQKYRTI 260

Query: 245 LNSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQV 304
           LNSKS+EESL+NLARWEPRHGKFRYRHPW  YLEIGSLNR+CA RLE+LNGYL+  + Q+
Sbjct: 261 LNSKSNEESLTNLARWEPRHGKFRYRHPWKVYLEIGSLNRECACRLEVLNGYLQTKRTQM 320

Query: 305 RFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKT 364
                  P   E  +    ICSE SSGLRELA+ LRKMVPPLTAK+HIEKAKIAAENLK+
Sbjct: 321 PSQQIHGPFQEECMK----ICSELSSGLRELAMGLRKMVPPLTAKAHIEKAKIAAENLKS 380

Query: 365 HLEEWRFEEVN---NAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSS 424
           HLEEWRFEE+N   NA+EIVH+ SL+ LL DTICCI KIVDSVQKLAS+AGFKAVE+ SS
Sbjct: 381 HLEEWRFEEMNNNKNALEIVHVASLSPLLLDTICCIVKIVDSVQKLASIAGFKAVEVPSS 440

Query: 425 VAPQQQKDLQDEDSLQPCHGTTILLAHHVITVDEGVPC 459
           VAP+     QD+ S+QPCHG  +L+AHHVI +DE  PC
Sbjct: 441 VAPE-----QDQGSMQPCHG-DVLVAHHVIMIDEESPC 468

BLAST of Lsi06G006300 vs. ExPASy TrEMBL
Match: A0A6J1JC53 (aluminum-activated malate transporter 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485449 PE=3 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 1.1e-193
Identity = 354/458 (77.29%), Postives = 397/458 (86.68%), Query Frame = 0

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           VKP+F KL AMAVELAT+TKKLAKDD+RRVVHALKVGLAITLVSLFYYFKPLYDGFGTST
Sbjct: 21  VKPIFTKLPAMAVELATMTKKLAKDDTRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 80

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           +WA++TV+VVFEFSVGGTLGRGLNRVMAT LAGALGFGAHYLASLGG+ GRPIMLGLFVF
Sbjct: 81  IWAVVTVVVVFEFSVGGTLGRGLNRVMATLLAGALGFGAHYLASLGGDTGRPIMLGLFVF 140

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           VLA ISTF RFFPKMKARYDYGLLIFILTFCMVS+SGYRDDEIAKLAYSR+LTILIGC F
Sbjct: 141 VLAAISTFIRFFPKMKARYDYGLLIFILTFCMVSLSGYRDDEIAKLAYSRMLTILIGCCF 200

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEE-EICKADMQKYRTI 244
           T+FVC+FV PVWAGTDLH+L+ +NI SLA FF GFG EYFGL QE+ E+ K DMQKYRT+
Sbjct: 201 TVFVCVFVCPVWAGTDLHHLVASNIDSLATFFHGFGAEYFGLLQEQGEVGKVDMQKYRTV 260

Query: 245 LNSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQV 304
           LNSKS+EESL+NLARWEPRHGKFRYRHPW  YLEIGSLNR+CA RLE+LNGYL+  + Q+
Sbjct: 261 LNSKSNEESLTNLARWEPRHGKFRYRHPWKVYLEIGSLNRECACRLEVLNGYLQTKRTQM 320

Query: 305 RFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKT 364
                  P   E  +    ICSE S+GLRELA+ LRKMVPPLTAK+HIEKAKIAAENLK+
Sbjct: 321 PSQQIHGPFQEECMK----ICSELSNGLRELAMGLRKMVPPLTAKAHIEKAKIAAENLKS 380

Query: 365 HLEEWRFEEVN---NAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSS 424
           HLEEWRFEE+N   NA+EIVH+ SL+ LL DTICCI KI DSVQKLAS+AGFKAVE+ SS
Sbjct: 381 HLEEWRFEEMNNNKNALEIVHVASLSPLLLDTICCIVKIADSVQKLASIAGFKAVEVPSS 440

Query: 425 VAPQQQKDLQDEDSLQPCHGTTILLAHHVITVDEGVPC 459
           VAP+     QD+ S+QPCHG  +L+AH VI +D+G PC
Sbjct: 441 VAPE-----QDQGSMQPCHG-AVLVAHQVIMIDKGAPC 468

BLAST of Lsi06G006300 vs. NCBI nr
Match: XP_038880572.1 (aluminum-activated malate transporter 2-like [Benincasa hispida])

HSP 1 Score: 742.7 bits (1916), Expect = 2.0e-210
Identity = 382/456 (83.77%), Postives = 408/456 (89.47%), Query Frame = 0

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           VKPVFAKL AMAV       KLAKDD RRVVHALKVGLAITLVS FYYFKPLYDGFGTST
Sbjct: 21  VKPVFAKLPAMAV-------KLAKDDPRRVVHALKVGLAITLVSFFYYFKPLYDGFGTST 80

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWAI+TVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGE GRPIMLGLFVF
Sbjct: 81  MWAIVTVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGETGRPIMLGLFVF 140

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           VL GISTF RFFPK+KARYDYG+LIFILTFCMVS+SGYRDDEIAKLA SRILTILIGC F
Sbjct: 141 VLGGISTFIRFFPKIKARYDYGMLIFILTFCMVSLSGYRDDEIAKLAISRILTILIGCCF 200

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
           T+ VCIFV+PVWAGTDLHYL+ TNIQSLAIFF+GFGVEYFGLS+E E+ K DMQKYRTIL
Sbjct: 201 TVIVCIFVRPVWAGTDLHYLVATNIQSLAIFFEGFGVEYFGLSKEGEVSKTDMQKYRTIL 260

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQV- 304
           NSKS+EESL+NLARWEPRHGKFRYRHPW QYL IGSLNR+CAYRLE+LNG LK NQFQ+ 
Sbjct: 261 NSKSNEESLTNLARWEPRHGKFRYRHPWKQYLVIGSLNRECAYRLEVLNGCLKANQFQMP 320

Query: 305 -RFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLK 364
            + +  F     +       ICSESS GLREL+L+LRKMVPPLTAK+HIEKAKIAAENLK
Sbjct: 321 SQVHGQFQEECRK-------ICSESSRGLRELSLALRKMVPPLTAKAHIEKAKIAAENLK 380

Query: 365 THLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVA 424
           +HLEEWRFEEVNNAMEIVH++SL SLLFDTICCIEKIVDSVQKLASMAGFKAVE+QS VA
Sbjct: 381 SHLEEWRFEEVNNAMEIVHVISLTSLLFDTICCIEKIVDSVQKLASMAGFKAVEVQSGVA 440

Query: 425 PQQQKDLQDEDSLQPCHGTTILLAHHVITVDEGVPC 459
           PQQQ DLQDE SLQPCHGTTILLAHHVIT+DE  PC
Sbjct: 441 PQQQNDLQDEGSLQPCHGTTILLAHHVITIDEETPC 462

BLAST of Lsi06G006300 vs. NCBI nr
Match: KAA0055909.1 (aluminum-activated malate transporter 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 711.8 bits (1836), Expect = 3.7e-201
Identity = 369/456 (80.92%), Postives = 403/456 (88.38%), Query Frame = 0

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +KP+FAKL   AV+LAT+TKKLAKDDSRRVVHA KVGLAI+LVSL YYFK LYDGFGTST
Sbjct: 21  LKPIFAKLSNKAVKLATMTKKLAKDDSRRVVHAFKVGLAISLVSLLYYFKALYDGFGTST 80

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWAI+TVIVV EFSVGGTLGRGLNRVMAT LAG LGFGAHYLASLGG+ GRPI+L  FVF
Sbjct: 81  MWAIVTVIVVVEFSVGGTLGRGLNRVMATLLAGGLGFGAHYLASLGGDTGRPIILAFFVF 140

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           +LA ISTFTRFFPK+KARYDYG+LIFILTFCMVS+SGYRDDEIAKLA SRILTILIGC F
Sbjct: 141 LLASISTFTRFFPKIKARYDYGMLIFILTFCMVSLSGYRDDEIAKLALSRILTILIGCCF 200

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
           TLFVCIFV+PVWAGTDLH L+  NIQSLA+FFQGFG E+F LSQ EE+ K DMQKYRTIL
Sbjct: 201 TLFVCIFVRPVWAGTDLHCLVANNIQSLALFFQGFGAEFFRLSQ-EEVSKDDMQKYRTIL 260

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVR 304
           NSKS+EESL+NLARWEPRHGKFRYRHPW QYL+IGSLNR+CAYRLELLNGYLK NQFQ+ 
Sbjct: 261 NSKSNEESLTNLARWEPRHGKFRYRHPWKQYLKIGSLNRECAYRLELLNGYLKTNQFQMP 320

Query: 305 FNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTH 364
                 P   E  +    ICSESS GLRELAL+LRKMV PLTAKSHIEKAK+AAE+LK+H
Sbjct: 321 SQQIHGPFKEECMK----ICSESSRGLRELALALRKMVLPLTAKSHIEKAKVAAEDLKSH 380

Query: 365 LEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVAPQ 424
           LEEWRFEEVNNAMEIV +VSLASLLFDTICCIEKIVDSVQ+LASMAGFKAVE+QSSVAP+
Sbjct: 381 LEEWRFEEVNNAMEIVQVVSLASLLFDTICCIEKIVDSVQELASMAGFKAVEVQSSVAPE 440

Query: 425 QQKDLQDED--SLQPCHGTTILLAHHVITVDEGVPC 459
           QQ DLQD+D  SLQP HG  +LLAHH IT+DE  PC
Sbjct: 441 QQMDLQDQDQYSLQPSHGAAVLLAHHAITIDEQSPC 471

BLAST of Lsi06G006300 vs. NCBI nr
Match: XP_004139138.1 (aluminum-activated malate transporter 2 [Cucumis sativus])

HSP 1 Score: 710.3 bits (1832), Expect = 1.1e-200
Identity = 366/460 (79.57%), Postives = 405/460 (88.04%), Query Frame = 0

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +KP+FAKL  ++V+LAT+ KKLAKDDSRRVVHALKVGLAI+LVSL YYFKPLYDGFGTST
Sbjct: 21  LKPIFAKLSTVSVKLATMAKKLAKDDSRRVVHALKVGLAISLVSLLYYFKPLYDGFGTST 80

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWAI+TVIVVFEFSVGGTLGRGLNRVMAT LAG LGFG HYLASLGG+ GRPI+L LFVF
Sbjct: 81  MWAIVTVIVVFEFSVGGTLGRGLNRVMATLLAGGLGFGTHYLASLGGDTGRPIILALFVF 140

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           +LA +STFTRFFPK+KARYDYGLLI ILTFCMVS+SGYRD+EIAKLA SRILTILIGC  
Sbjct: 141 ILASVSTFTRFFPKIKARYDYGLLILILTFCMVSLSGYRDEEIAKLALSRILTILIGCCV 200

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
           TL VCIFV+PVWAGTDLH L+  NIQSLA+FFQGFG E+FGLSQE E+   DMQKYRTIL
Sbjct: 201 TLIVCIFVRPVWAGTDLHCLVANNIQSLALFFQGFGAEFFGLSQEGEVSNDDMQKYRTIL 260

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQV- 304
           NSKS+EESL+NLARWEPRHGKFRYRHPW QYL+IGSLNR+CAYRLELLNGYLK NQFQ+ 
Sbjct: 261 NSKSNEESLTNLARWEPRHGKFRYRHPWKQYLKIGSLNRECAYRLELLNGYLKTNQFQMP 320

Query: 305 --RFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENL 364
             + +  F    ++       ICSESS GLRELAL+LRKMV PLTAKSHIEKAKIAAENL
Sbjct: 321 SQQIHGQFKEECMK-------ICSESSRGLRELALALRKMVLPLTAKSHIEKAKIAAENL 380

Query: 365 KTHLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSV 424
           K+HLEEWRFEEVNNAMEIV +VSLASLLFDTICCIEKIVDSVQ+LASMAGFKAVE+QSSV
Sbjct: 381 KSHLEEWRFEEVNNAMEIVQVVSLASLLFDTICCIEKIVDSVQELASMAGFKAVEVQSSV 440

Query: 425 APQQQKDLQDED--SLQP-CHGTTILLAHHVITVDEGVPC 459
           AP+QQ DLQD+D  +LQP  HG  +LLAHH IT+DE  PC
Sbjct: 441 APEQQMDLQDQDQYALQPLSHGAAVLLAHHAITIDEQSPC 473

BLAST of Lsi06G006300 vs. NCBI nr
Match: XP_008450297.1 (PREDICTED: aluminum-activated malate transporter 2-like [Cucumis melo])

HSP 1 Score: 709.5 bits (1830), Expect = 1.8e-200
Identity = 368/456 (80.70%), Postives = 402/456 (88.16%), Query Frame = 0

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +KP+FAKL   AV+LAT+TKKLAKDDSRRVVHA KVGLAI+LVSL YYFK LYDGFGTST
Sbjct: 21  LKPIFAKLSNKAVKLATMTKKLAKDDSRRVVHAFKVGLAISLVSLLYYFKALYDGFGTST 80

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWAI+TVIVV EFSVGGTLGRGLNRVMAT LAG LGFGAHYLASL G+ GRPI+L  FVF
Sbjct: 81  MWAIVTVIVVVEFSVGGTLGRGLNRVMATLLAGGLGFGAHYLASLSGDTGRPIILAFFVF 140

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           +LA ISTFTRFFPK+KARYDYG+LIFILTFCMVS+SGYRDDEIAKLA SRILTILIGC F
Sbjct: 141 LLASISTFTRFFPKIKARYDYGMLIFILTFCMVSLSGYRDDEIAKLALSRILTILIGCCF 200

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
           TLFVCIFV+PVWAGTDLH L+  NIQSLA+FFQGFG E+F LSQ EE+ K DMQKYRTIL
Sbjct: 201 TLFVCIFVRPVWAGTDLHCLVANNIQSLALFFQGFGAEFFRLSQ-EEVSKDDMQKYRTIL 260

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVR 304
           NSKS+EESL+NLARWEPRHGKFRYRHPW QYL+IGSLNR+CAYRLELLNGYLK NQFQ+ 
Sbjct: 261 NSKSNEESLTNLARWEPRHGKFRYRHPWKQYLKIGSLNRECAYRLELLNGYLKTNQFQMP 320

Query: 305 FNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTH 364
                 P   E  +    ICSESS GLRELAL+LRKMV PLTAKSHIEKAK+AAE+LK+H
Sbjct: 321 SQQIHGPFKEECMK----ICSESSRGLRELALALRKMVLPLTAKSHIEKAKVAAEDLKSH 380

Query: 365 LEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVAPQ 424
           LEEWRFEEVNNAMEIV +VSLASLLFDTICCIEKIVDSVQ+LASMAGFKAVE+QSSVAP+
Sbjct: 381 LEEWRFEEVNNAMEIVQVVSLASLLFDTICCIEKIVDSVQELASMAGFKAVEVQSSVAPE 440

Query: 425 QQKDLQDED--SLQPCHGTTILLAHHVITVDEGVPC 459
           QQ DLQD+D  SLQP HG  +LLAHH IT+DE  PC
Sbjct: 441 QQMDLQDQDQYSLQPSHGAAVLLAHHAITIDEQSPC 471

BLAST of Lsi06G006300 vs. NCBI nr
Match: XP_022960622.1 (aluminum-activated malate transporter 2-like [Cucurbita moschata])

HSP 1 Score: 689.5 bits (1778), Expect = 2.0e-194
Identity = 359/458 (78.38%), Postives = 398/458 (86.90%), Query Frame = 0

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           VKP+FAKL A+AVELAT+T KLAKDD+RRVVHALKVGLAITLVSLFYYFKPLYDGFGTST
Sbjct: 21  VKPIFAKLPAIAVELATMTTKLAKDDTRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 80

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWA++TVIVVFEFSVGGTLGRGLNRVMAT LAGALGFGAHYLASLGG+ GRPIMLGLFVF
Sbjct: 81  MWAVVTVIVVFEFSVGGTLGRGLNRVMATLLAGALGFGAHYLASLGGDTGRPIMLGLFVF 140

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           VLA ISTF RFFPKMKARYDYGLLIFILTFCMVS+SGYRDDEIAKLAYSR+LTILIGC F
Sbjct: 141 VLAAISTFIRFFPKMKARYDYGLLIFILTFCMVSLSGYRDDEIAKLAYSRMLTILIGCCF 200

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEE-EICKADMQKYRTI 244
           T+FVC+FV PVWAGTDLH+L+  NI+SLA FF GFG EYFGL QE+ E+ K DMQKYRTI
Sbjct: 201 TVFVCVFVCPVWAGTDLHHLVANNIESLATFFHGFGAEYFGLLQEQGEVGKVDMQKYRTI 260

Query: 245 LNSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQV 304
           LNSKS+EESL+NLARWEPRHGKFRYRHPW  YLEIGSLNR+CA RLE+LNGYL+  + Q+
Sbjct: 261 LNSKSNEESLTNLARWEPRHGKFRYRHPWKVYLEIGSLNRECACRLEVLNGYLQTKRTQM 320

Query: 305 RFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKT 364
                  P   E  +    ICSE SSGLRELA+ LRKMVPPLTAK+HIEKAKIAAENLK+
Sbjct: 321 PSQQIHGPFQEECMK----ICSELSSGLRELAMGLRKMVPPLTAKAHIEKAKIAAENLKS 380

Query: 365 HLEEWRFEEVN---NAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSS 424
           HLEEWRFEE+N   NA+EIVH+ SL+ LL DTICCI KIVDSVQKLAS+AGFKAVE+ SS
Sbjct: 381 HLEEWRFEEMNNNKNALEIVHVASLSPLLLDTICCIVKIVDSVQKLASIAGFKAVEVPSS 440

Query: 425 VAPQQQKDLQDEDSLQPCHGTTILLAHHVITVDEGVPC 459
           VAP+     QD+ S+QPCHG  +L+AHHVI +DE  PC
Sbjct: 441 VAPE-----QDQGSMQPCHG-DVLVAHHVIMIDEESPC 468

BLAST of Lsi06G006300 vs. TAIR 10
Match: AT1G08440.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 367.9 bits (943), Expect = 1.2e-101
Identity = 198/437 (45.31%), Postives = 290/437 (66.36%), Query Frame = 0

Query: 18  ELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTSTMWAIITVIVVFEF 77
           E+    +++ K+D RRVVHA KVGLA+ LVS FYY++PLYD FG + MWA++TV+VVFEF
Sbjct: 6   EIVREGRRVGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVVFEF 65

Query: 78  SVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFP 137
           SVG TLG+GLNR +AT +AG LG GAH+LASL G    PI+L +FVFVLA +STF RFFP
Sbjct: 66  SVGATLGKGLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVRFFP 125

Query: 138 KMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWA 197
           ++KARYDYG+LIFILTF ++SVSG+R+DEI  LA+ R+ T+++G    + + IFV PVWA
Sbjct: 126 RVKARYDYGVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCPVWA 185

Query: 198 GTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKAD-----MQKYRTILNSKSSEES 257
           G DLH LL +N  +L+ F Q FG EYF  +++ +I + +     +++Y+++LNSKS+EE+
Sbjct: 186 GQDLHSLLASNFDTLSHFLQEFGDEYFEATEDGDIKEVEKRRRNLERYKSVLNSKSNEEA 245

Query: 258 LSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVRFNANFDPR 317
           L+N A+WEPRHG+FR+RHPW QYL +G+L R  AYR++ LN  +  +   ++   +   +
Sbjct: 246 LANFAKWEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSD---MQIPMDIKKK 305

Query: 318 LLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTHLEEWRFEE 377
           + E  R    + SES   ++E+++SL+ M    +   H+  ++ A + L T L+     +
Sbjct: 306 IEEPLR---RMSSESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKSGILND 365

Query: 378 VNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQS-----SVAPQQQK 437
           V   ++++ +++  SLL D +   EKI +SV +LAS A FK  +  S     S+      
Sbjct: 366 V-EPLQMISLMTTVSLLIDIVNLTEKISESVHELASAAKFKNKKKPSKSNSGSIGQAMPN 425

Query: 438 DLQDEDSLQPCHGTTIL 445
              D+D     H  TIL
Sbjct: 426 KSHDDDD----HVVTIL 431

BLAST of Lsi06G006300 vs. TAIR 10
Match: AT2G27240.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 365.2 bits (936), Expect = 7.9e-101
Identity = 196/428 (45.79%), Postives = 272/428 (63.55%), Query Frame = 0

Query: 18  ELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTSTMWAIITVIVVFEF 77
           E+    +++AK+D RRVVH+ KVGL + LVS FYY++PLYD FG + MWA++TV+VVFEF
Sbjct: 6   EIVREGRRVAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVVFEF 65

Query: 78  SVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFP 137
           SVG TLG+GLNRV AT  AG LG GAH+LAS+ G  G PI+L +FVFV A +STF RFFP
Sbjct: 66  SVGATLGKGLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVRFFP 125

Query: 138 KMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWA 197
           ++KARYDY LLIFILTF ++SVSG+R++++ KL + RI T++IG    + + IFV PVWA
Sbjct: 126 RVKARYDYSLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCPVWA 185

Query: 198 GTDLHYLLITNIQSLAIFFQG--------------------------FGVEYFGL----- 257
           G DLH L+ +N + L+ F  G                          FG +Y  +     
Sbjct: 186 GQDLHSLIASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVVENDG 245

Query: 258 SQEEEICKADMQKYRTILNSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCA 317
           ++E +  K D   Y+++LNSKS+EESL+N A+WEP HG+FR+RHPW QYL +G L R CA
Sbjct: 246 AKEVDKRKKDFDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELIRQCA 305

Query: 318 YRLELLNGYLK-PNQFQVRFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPL 377
           YR+  LN YL   NQ  V         L  +         ES   ++E+++SL+KM  P 
Sbjct: 306 YRIHALNSYLNADNQVSVDIKKKLGEPLRRM-------SLESGKAMKEMSISLKKMTKPS 365

Query: 378 TAKSHIEKAKIAAENLKTHLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQK 414
           ++  H++ AK A ++L   L     +EV   +E+V +++  SLL D I   EKI++S+ +
Sbjct: 366 SSDLHVQNAKSACKSLTNLLNSGILKEV-EPLELVSLLTAISLLIDIINLTEKILESLHE 425

BLAST of Lsi06G006300 vs. TAIR 10
Match: AT3G11680.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 353.6 bits (906), Expect = 2.4e-97
Identity = 187/391 (47.83%), Postives = 263/391 (67.26%), Query Frame = 0

Query: 24  KKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTSTMWAIITVIVVFEFSVGGTL 83
           +K AKDD RR++H++KVG+A+TLVSL YY +PLY  FG + MWAI+TV+VVFEF+VGGTL
Sbjct: 35  QKFAKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAILTVVVVFEFTVGGTL 94

Query: 84  GRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFPKMKARY 143
            +GLNR  AT +AGALG GA +LA   G  G PI+LG+ VF L   +TF+RFFP++K RY
Sbjct: 95  SKGLNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAAATFSRFFPRIKQRY 154

Query: 144 DYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWAGTDLHY 203
           DYG LIFILTF  V++SGYR DEI  +AY R+ TILIG    + V IF+ PVWAG DLH 
Sbjct: 155 DYGALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVSIFICPVWAGEDLHK 214

Query: 204 LLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTILNSKSSEESLSNLARWEPRH 263
           ++  NI  LA + +GF  EYF   +  +   + +++Y++IL SKS+E+SL+NLARWEP H
Sbjct: 215 MIANNINKLAKYLEGFEGEYFQPEKISKETSSCVREYKSILTSKSTEDSLANLARWEPGH 274

Query: 264 GKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVRFNANFDPRLLELYRLDFYI 323
           G+FR RHPW +YL+I  L R CA  LE+LNGY+  N    +    F+ ++ E       +
Sbjct: 275 GRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSND---KAPQEFESKIQEPITT---M 334

Query: 324 CSESSSGLRELALSLRKM-VPPLTAKSHIEKAKIAAENLKTHLEEWRFEEVNNAMEIVHI 383
             E    L+ +A S++ M        +HI+ +K A +NLK  L+    E   + +EI+  
Sbjct: 335 SREVGEALKAIAKSIKTMRNDSACVNAHIDNSKKAIKNLKIALKSSYPETYKDLLEIIPG 394

Query: 384 VSLASLLFDTICCIEKIVDSVQKLASMAGFK 414
           V++AS+L + + C+EKI ++V++ + +A FK
Sbjct: 395 VTMASILIEVVNCVEKIYEAVEEFSGLAHFK 419

BLAST of Lsi06G006300 vs. TAIR 10
Match: AT1G08430.1 (aluminum-activated malate transporter 1 )

HSP 1 Score: 344.7 bits (883), Expect = 1.1e-94
Identity = 189/437 (43.25%), Postives = 278/437 (63.62%), Query Frame = 0

Query: 25  KLAKDDSRRVVHALKVGLAITLVSLFYY---FKPLYDGFGTSTMWAIITVIVVFEFSVGG 84
           ++  +D RR++HA KVGLA+ LVS FYY   F P  D FG + MWA++TV+VVFEFSVG 
Sbjct: 13  RVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTVVVVFEFSVGA 72

Query: 85  TLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFPKMKA 144
           TLG+GLNR +AT +AG LG GAH LA L G    PI+L + VFV A +STF RFFP +K 
Sbjct: 73  TLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALSTFVRFFPWVKT 132

Query: 145 RYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWAGTDL 204
           ++DYG+LIFILTF ++S+SG+RD+EI  LA SR+ T++IG    + + IFV PVWAG DL
Sbjct: 133 KFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIFVCPVWAGQDL 192

Query: 205 HYLLITNIQSLAIFFQGFGVEYF-----GLSQEEEICKADMQKYRTILNSKSSEESLSNL 264
           H LL +N  +L+ F Q FG EYF     G  +  E  K ++++Y+++L+SKS EE+L+N 
Sbjct: 193 HSLLASNFDTLSHFLQDFGDEYFEAREKGDYKVVEKRKKNLERYKSVLDSKSDEEALANY 252

Query: 265 ARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPN-QFQVRFNANFDPRLLE 324
           A WEP HG+FR+RHPW QY+ +G+L R CAYR++ LN Y+  + Q  V      +  L  
Sbjct: 253 AEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDIKKKLETPLRR 312

Query: 325 LYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTHLEEWRFEEVNN 384
           +        SES + ++E+++SL++M+   ++  H+  ++ A ++L T L+     +V  
Sbjct: 313 M-------SSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKSGILNDV-E 372

Query: 385 AMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVAPQQQKDLQDEDSL 444
            ++++ +++  S+L D +   EKI +SV +LAS A FK     + + P    +  D  S+
Sbjct: 373 PLQMISLMTTVSMLIDIVNLTEKISESVHELASAARFK-----NKMRPTVLYEKSDSGSI 432

Query: 445 QPCHGTTILLAHHVITV 453
                      HHV+TV
Sbjct: 433 GRAMPIDSHEDHHVVTV 436

BLAST of Lsi06G006300 vs. TAIR 10
Match: AT4G00910.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 260.8 bits (665), Expect = 2.1e-69
Identity = 167/456 (36.62%), Postives = 257/456 (56.36%), Query Frame = 0

Query: 8   VFAKLLAMAVELATITK------KLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFG 67
           ++ K L M V +  + K      ++  DD  +VVH LKVGLA++LVS+FYY +PLYDG G
Sbjct: 35  LWLKDLVMKVIMERVAKFMRKAWRIGADDPAKVVHCLKVGLALSLVSIFYYMRPLYDGVG 94

Query: 68  TSTMWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGL 127
            + MWAI+TV+VVFE +VG T  + +NRV+AT LAG+LG   H++A+  G+     ++G 
Sbjct: 95  GNAMWAIMTVVVVFESNVGATFCKCVNRVVATILAGSLGIAVHWVATQSGK-AEVFVIGC 154

Query: 128 FVFVLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIG 187
            VF+ A  +T++RF P  KAR+DYG +IFILTF +VSV GYR D++ +LA  R+ TI IG
Sbjct: 155 SVFLFAFAATYSRFVPSFKARFDYGAMIFILTFSLVSVGGYRVDKLVELAQQRVSTIAIG 214

Query: 188 CFFTLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQ------EEEICKA 247
               + + +F  P+WAG+ LH L+  N++ LA    G   EYF  ++      E+E    
Sbjct: 215 TSICIIITVFFCPIWAGSQLHRLIERNLEKLADSLDGCVAEYFKENEVSTNRNEDENTNM 274

Query: 248 DMQKYRTILNSKSSEESL------------SNLARWEPRHGKFRYRHPWNQYLEIGSLNR 307
            +Q ++ +LNSK +EE++            +NLARWEP HG F +RHPW  Y++IG+  R
Sbjct: 275 KLQGFKCVLNSKGTEEAMPLIRFSGFSFSQANLARWEPAHGSFNFRHPWKLYVKIGAAMR 334

Query: 308 DCAYRLELLNGYLK-----PNQFQVRFN-----------------ANFDPRLLELYRLDF 367
            CAY LE L+  +      P+Q +  F                  A+      +  ++DF
Sbjct: 335 RCAYCLENLSICINYETEAPDQVKNHFGEACMKLSSASSKILRELADMMKNTRKSSKMDF 394

Query: 368 YICSESSSGLRELALSLRKMVPPLTAKSH----IEKAKIAAENLKTHLEEWRFEEVNNAM 414
            +  + +S ++EL  +L K VP  T K       E+ K+ +E   T +         +  
Sbjct: 395 LV-FDMNSAVQELQETL-KNVPIETNKPEEVPSEEENKVDSEERTTSM---------SLH 454

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SJE81.7e-10045.31Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2... [more]
Q9XIN11.1e-9945.79Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7... [more]
Q9SRM93.4e-9647.83Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8... [more]
Q9SJE91.6e-9343.25Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1... [more]
Q76LB14.1e-7840.79Aluminum-activated malate transporter 1 OS=Triticum aestivum OX=4565 GN=ALMT1 PE... [more]
Match NameE-valueIdentityDescription
A0A5A7UR041.8e-20180.92Aluminum-activated malate transporter 2-like OS=Cucumis melo var. makuwa OX=1194... [more]
A0A0A0M3015.2e-20179.57Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G630850 PE=3 SV=1[more]
A0A1S3BNX08.9e-20180.70aluminum-activated malate transporter 2-like OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1H7Y19.5e-19578.38aluminum-activated malate transporter 2-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JC531.1e-19377.29aluminum-activated malate transporter 2-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_038880572.12.0e-21083.77aluminum-activated malate transporter 2-like [Benincasa hispida][more]
KAA0055909.13.7e-20180.92aluminum-activated malate transporter 2-like [Cucumis melo var. makuwa][more]
XP_004139138.11.1e-20079.57aluminum-activated malate transporter 2 [Cucumis sativus][more]
XP_008450297.11.8e-20080.70PREDICTED: aluminum-activated malate transporter 2-like [Cucumis melo][more]
XP_022960622.12.0e-19478.38aluminum-activated malate transporter 2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G08440.11.2e-10145.31Aluminium activated malate transporter family protein [more]
AT2G27240.17.9e-10145.79Aluminium activated malate transporter family protein [more]
AT3G11680.12.4e-9747.83Aluminium activated malate transporter family protein [more]
AT1G08430.11.1e-9443.25aluminum-activated malate transporter 1 [more]
AT4G00910.12.1e-6936.62Aluminium activated malate transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 351..371
NoneNo IPR availablePANTHERPTHR31086ALUMINUM-ACTIVATED MALATE TRANSPORTER 10coord: 6..420
NoneNo IPR availablePANTHERPTHR31086:SF53ALUMINUM ACTIVATED MALATE TRANSPORTER FAMILY PROTEINcoord: 6..420
IPR020966Aluminum-activated malate transporterPFAMPF11744ALMTcoord: 25..366
e-value: 1.1E-126
score: 423.5

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G006300.1Lsi06G006300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0015743 malate transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane