Lsi06G005660 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G005660
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Isoleucine N-monooxygenase 1
Location	chr06: 7352858 .. 7355564 (-)
RNA-Seq Expression	Lsi06G005660
Synteny	Lsi06G005660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGTTTTGCTATTCATTAGTGAATCATCATATCAAAACTATGCAAAAGAATCCATAGCCATAACCTTCATTGCTATCATTTTATTCATATCTTCCATTATTTATGTTATTAGGTTTTCTCAATATTACTGCAAAAGACCTCTCTCAATCCGGCGGCTTCCACCTGGCCCAAAGCCATGGCCTCTCCTTGGCTACCTTCCAGCAATGTTGAGCAACAAAAACTTGCCAACACACCATTGGATCCATTCAATTATGAAGCAATTCAATACTGAAATTGCTTGCATCCGTCTCGGATCAAATACTCATATAATCCCTGTGACTTCTCCAGAACTCGCTCTTGAATTTCTAAAGACACACGATTCTGTCTTCAGATCACGACCTATTTCCATGACTGCTGAGATAGTGAGTCACGGATATCTCAGTACAGTTCTTTCACCCATGGGGGATCAATGGAAGAAAATGAGAAGGATACTCGTTTCCCACGTACTTAATTCTTCGACCCTCCATCAAATGCTTGGCCAAAGAACAAAGGAAGCTGATATTCTTCTATGCTATATTTTCAACTTATCTAGAAACGGTGAAGCCATCAACATAAAAAGCATTGTACGACATTACTGCAGCAACATTGTTAGAAGAATGTTGTTCAATAAAAGATACTATGGGAAAGGGAGGGAAGATGGGGGGCCAACTTTTGAAGAAGAAGACCATAATCAAGCATTGTTGTCCATTCTTAGGCATGTTAATGCATTTTCTATATCTGAGTTTATACCTTTTTTGAAGCTATTTGATCTTGATGGACATGAGAAGATAGTAAGAAGGGCTTTGAAAGTCATAAGAAACCATGACGAACCCATTATAGAGGAAAGAGTGCAAGAATGGAGAGATGGGAAGAAAAAGGATGTGGAGGACATTCTAGATATCCTTATCTCACTCAAAAATGAGAATGAAAAATCATTGTTGTCAATCGAAGAGATTAAGGCTCAAGTTACGGTAATGTATAATATATATATATATATATATATTTTTTATCATATTATAGAGGATTTCCGTTGAAAATATTAAGAGACCTCACAATCATATATGAGTTACTCCTCTCATTATTGTCAATTGGTTTTGAGCTGAAATTTCTTCTTTATTTAATTAAAAAACTTAGGTGCATCTCAGGCTATACCTATCTTTGTGTATCCTACCAAATTATCCAATTATAACTTGCCATAGTGGCAAATTAACTTTATTTCTTTTTTCAAAATATTTTAATACTTTTTGTGTGTGTATAATGAGCGTAGGATGCATACAGATGGGTGCATCTTGGGATGCACCTAATTATTGATCTTATTTAATTTGGTATCATAGGACATGAATCCCAAATAGGTATTCGATTCAAACTTGAGATCCAATTTAAGAATGCTAAACTCAAAGAGATGTCATCTTCAAGAGGACATGCTGAGGATTCTACATCGAAAACATCATGAAGACCTCATAGTCTTTATAAGATACATGGATTATTAATTTATTACCAATTAGTTTTGTGATGGAACTTCATGTTTATCTTATACATATGACAGATACAAGCAGATTTAGAGCATTTCCTTATGTTATTGAAATTACTTTTTTCCCATCATATGGTAGGATCTGCAACTTGCAAGCATAGATAATCCTTCAAATGCAGTGGAATGGGCAATGGCAGAGTTACTTAATCAACCAAAACTTCTCCAAAAAGCTGTTGAAGAATTAGACGAAGTAGTTGGAAGAGATAGGCTGGTTCAAGAATTTGATGTACCTAATCTCAAGTATCTTACAGCTTGTATTAGAGAATCTTTTCGACTTCATCCATTTTCACCATTCAATGTTCCTCACGTTTCCAACTCTGATATTGTTGTAGCTGGCTACTTCATCCCTAAAGGTAGCCAAGTATTACTAAGTCGTCTTGGTCTCGGTCGAAACTCAAGGATTTGGGAGGATCTAATGAGATTTAATCCAGAGCGTCATCTAAAAAATGGGACAATTGAACTGGGAATTTCAGAACCCGATCTACGATTCGTCACCTTCACTAGAGGGAGGAGAGGATGCATTGGTACATCACTTGGCACTAACATGACCATGATGCTATTTGCTAGACTTGTACAAGGATTTTCATGGAGCTTATTGTCAGGAATCACAAAGATCGATTTCTCCAAAACCGATGAATTATCTCTTAAGCCATTACATCTACATGCAGAGCCACGTTTCTCATATTATGTATCCAACTTATTAATAATTAAGCATATAGAGCTAACTAAAATAATATTGTCTACGAAATTTAAAAAAAAAAAAAAAAAACAGGTAGTTAATTGAGAATTCCTTCTGCTTTCCTTGAGGCGTTCATCTATGTTCTATCATTCATTGCAAAAGTAATGTGTTATAAAACCCTAGACCCTATAATACGAGTTTTTTTTTATTTTTTTTTTAATTTTTTTTATTTCCTTCATCTACTTCTTCTTGATGTTTTTTGCAAAATGGGGTTGCGTGCGTCTCTCTCGTAGAAATTTTTCTTTTCGATTTCACTTTCAAATTTTCTCCTTCAAATTCGTCATAGTGAATGAATCCTTCTCCTTTTTTTTTGTTGTTTCTTCTTGTTGTTTTAATTGGATTTCAGACCATTTTTCTCATCGATTGTAGTTTTTTGTTCTTGATCATAGATCTTTCTAAATTCAATAACCGATAG

mRNA sequence

ATGAAAGTTTTGCTATTCATTAGTGAATCATCATATCAAAACTATGCAAAAGAATCCATAGCCATAACCTTCATTGCTATCATTTTATTCATATCTTCCATTATTTATGTTATTAGGTTTTCTCAATATTACTGCAAAAGACCTCTCTCAATCCGGCGGCTTCCACCTGGCCCAAAGCCATGGCCTCTCCTTGGCTACCTTCCAGCAATGTTGAGCAACAAAAACTTGCCAACACACCATTGGATCCATTCAATTATGAAGCAATTCAATACTGAAATTGCTTGCATCCGTCTCGGATCAAATACTCATATAATCCCTGTGACTTCTCCAGAACTCGCTCTTGAATTTCTAAAGACACACGATTCTGTCTTCAGATCACGACCTATTTCCATGACTGCTGAGATAGTGAGTCACGGATATCTCAGTACAGTTCTTTCACCCATGGGGGATCAATGGAAGAAAATGAGAAGGATACTCGTTTCCCACGTACTTAATTCTTCGACCCTCCATCAAATGCTTGGCCAAAGAACAAAGGAAGCTGATATTCTTCTATGCTATATTTTCAACTTATCTAGAAACGGTGAAGCCATCAACATAAAAAGCATTGTACGACATTACTGCAGCAACATTGTTAGAAGAATGTTGTTCAATAAAAGATACTATGGGAAAGGGAGGGAAGATGGGGGGCCAACTTTTGAAGAAGAAGACCATAATCAAGCATTGTTGTCCATTCTTAGGCATGTTAATGCATTTTCTATATCTGAGTTTATACCTTTTTTGAAGCTATTTGATCTTGATGGACATGAGAAGATAGATCTGCAACTTGCAAGCATAGATAATCCTTCAAATGCAGTGGAATGGGCAATGGCAGAGTTACTTAATCAACCAAAACTTCTCCAAAAAGCTGTTGAAGAATTAGACGAAGTAGTTGGAAGAGATAGGCTGGTTCAAGAATTTGATGTACCTAATCTCAAGTATCTTACAGCTTGTATTAGAGAATCTTTTCGACTTCATCCATTTTCACCATTCAATGTTCCTCACGTTTCCAACTCTGATATTGTTGTAGCTGGCTACTTCATCCCTAAAGGTAGCCAAGTATTACTAAGTCGTCTTGGTCTCGGTCGAAACTCAAGGATTTGGGAGGATCTAATGAGATTTAATCCAGAGCGTCATCTAAAAAATGGGACAATTGAACTGGGAATTTCAGAACCCGATCTACGATTCGTCACCTTCACTAGAGGGAGGAGAGGATGCATTGGTACATCACTTGGCACTAACATGACCATGATGCTATTTGCTAGACTTGTACAAGGATTTTCATGGAGCTTATTGTCAGGAATCACAAAGATCGATTTCTCCAAAACCGATGAATTATCTCTTAAGCCATTACATCTACATGCAGAGCCACGTTTCTCATATTATACCATTTTTCTCATCGATTGTAGTTTTTTGTTCTTGATCATAGATCTTTCTAAATTCAATAACCGATAG

Coding sequence (CDS)

ATGAAAGTTTTGCTATTCATTAGTGAATCATCATATCAAAACTATGCAAAAGAATCCATAGCCATAACCTTCATTGCTATCATTTTATTCATATCTTCCATTATTTATGTTATTAGGTTTTCTCAATATTACTGCAAAAGACCTCTCTCAATCCGGCGGCTTCCACCTGGCCCAAAGCCATGGCCTCTCCTTGGCTACCTTCCAGCAATGTTGAGCAACAAAAACTTGCCAACACACCATTGGATCCATTCAATTATGAAGCAATTCAATACTGAAATTGCTTGCATCCGTCTCGGATCAAATACTCATATAATCCCTGTGACTTCTCCAGAACTCGCTCTTGAATTTCTAAAGACACACGATTCTGTCTTCAGATCACGACCTATTTCCATGACTGCTGAGATAGTGAGTCACGGATATCTCAGTACAGTTCTTTCACCCATGGGGGATCAATGGAAGAAAATGAGAAGGATACTCGTTTCCCACGTACTTAATTCTTCGACCCTCCATCAAATGCTTGGCCAAAGAACAAAGGAAGCTGATATTCTTCTATGCTATATTTTCAACTTATCTAGAAACGGTGAAGCCATCAACATAAAAAGCATTGTACGACATTACTGCAGCAACATTGTTAGAAGAATGTTGTTCAATAAAAGATACTATGGGAAAGGGAGGGAAGATGGGGGGCCAACTTTTGAAGAAGAAGACCATAATCAAGCATTGTTGTCCATTCTTAGGCATGTTAATGCATTTTCTATATCTGAGTTTATACCTTTTTTGAAGCTATTTGATCTTGATGGACATGAGAAGATAGATCTGCAACTTGCAAGCATAGATAATCCTTCAAATGCAGTGGAATGGGCAATGGCAGAGTTACTTAATCAACCAAAACTTCTCCAAAAAGCTGTTGAAGAATTAGACGAAGTAGTTGGAAGAGATAGGCTGGTTCAAGAATTTGATGTACCTAATCTCAAGTATCTTACAGCTTGTATTAGAGAATCTTTTCGACTTCATCCATTTTCACCATTCAATGTTCCTCACGTTTCCAACTCTGATATTGTTGTAGCTGGCTACTTCATCCCTAAAGGTAGCCAAGTATTACTAAGTCGTCTTGGTCTCGGTCGAAACTCAAGGATTTGGGAGGATCTAATGAGATTTAATCCAGAGCGTCATCTAAAAAATGGGACAATTGAACTGGGAATTTCAGAACCCGATCTACGATTCGTCACCTTCACTAGAGGGAGGAGAGGATGCATTGGTACATCACTTGGCACTAACATGACCATGATGCTATTTGCTAGACTTGTACAAGGATTTTCATGGAGCTTATTGTCAGGAATCACAAAGATCGATTTCTCCAAAACCGATGAATTATCTCTTAAGCCATTACATCTACATGCAGAGCCACGTTTCTCATATTATACCATTTTTCTCATCGATTGTAGTTTTTTGTTCTTGATCATAGATCTTTCTAAATTCAATAACCGATAG

Protein sequence

MKVLLFISESSYQNYAKESIAITFIAIILFISSIIYVIRFSQYYCKRPLSIRRLPPGPKPWPLLGYLPAMLSNKNLPTHHWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFRSRPISMTAEIVSHGYLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQMLGQRTKEADILLCYIFNLSRNGEAINIKSIVRHYCSNIVRRMLFNKRYYGKGREDGGPTFEEEDHNQALLSILRHVNAFSISEFIPFLKLFDLDGHEKIDLQLASIDNPSNAVEWAMAELLNQPKLLQKAVEELDEVVGRDRLVQEFDVPNLKYLTACIRESFRLHPFSPFNVPHVSNSDIVVAGYFIPKGSQVLLSRLGLGRNSRIWEDLMRFNPERHLKNGTIELGISEPDLRFVTFTRGRRGCIGTSLGTNMTMMLFARLVQGFSWSLLSGITKIDFSKTDELSLKPLHLHAEPRFSYYTIFLIDCSFLFLIIDLSKFNNR

Homology

[Close]

BLAST of Lsi06G005660 vs. ExPASy Swiss-Prot
Match: A0A068Q605 (Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 2.0e-131
Identity = 238/513 (46.39%), Postives = 336/513 (65.50%), Query Frame = 0


Query: 25  IAIILFISSIIYVIRFSQYYCKRPLSIRRLPPGPKPWPLLGYLPAMLSNKNLPTHHWIHS 84
           +A I+F+  + ++++    + K  + +  LPPGP PWP++G LP M  N+  P H WIHS
Sbjct: 20  LAFIIFMFLVKFILK---THNKNSVPVVPLPPGPSPWPIVGSLPEMWRNR--PAHRWIHS 79

Query: 85  IMKQFNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFRSRPISMTAEIVSHGYLSTV 144
           +MK+ NT+IACIRLG N H+IPVTSPE+A EFLK +D+VF SRP++M  + +S GYL+TV
Sbjct: 80  LMKKLNTDIACIRLG-NVHVIPVTSPEIAREFLKKNDAVFASRPVTMATKTLSSGYLTTV 139

Query: 145 LSPMGDQWKKMRRILVSHVLNSSTLHQMLGQRTKEADILLCYIFN---LSRNGEAINIKS 204
           + P GDQW+KMRR+LV+   N S +H +LG+R +EAD L+ +++N    ++NG  +N++ 
Sbjct: 140 VGPWGDQWRKMRRVLVAEAFNPSRVHWLLGKRNEEADNLVKFLYNQCSANQNGAVVNVRI 199

Query: 205 IVRHYCSNIVRRMLFNKRYYGKGREDGGPTFEEEDHNQALLSILRHVNAFSISEFIPFLK 264
             + Y  +I+R+M+FN+ Y+GKGREDGGP  EEE+H  ALL++L +  AF +S+++P+L+
Sbjct: 200 AAQFYSGSIMRKMIFNRTYFGKGREDGGPGVEEEEHVSALLTLLTYAYAFCVSDYLPWLR 259

Query: 265 LFDLDGHEK--------------------------------------------------- 324
           +FD+DGHEK                                                   
Sbjct: 260 VFDIDGHEKKVRKAMNIVKKHQEPIVNERLQEWRDGKRNEPDDLLDVFISLKDANGQPLL 319

Query: 325 ---------IDLQLASIDNPSNAVEWAMAELLNQPKLLQKAVEELDEVVGRDRLVQEFDV 384
                     +LQL+++D+P N  EWA+ E+LNQP++L+KA EELD VVG+  LVQE  V
Sbjct: 320 SDEEIKAQTTELQLSTVDSPFNIAEWALTEMLNQPEMLKKAEEELDRVVGKKTLVQESHV 379

Query: 385 PNLKYLTACIRESFRLHPFSPFNVPHVSNSDIVVAGYFIPKGSQVLLSRLGLGRNSRIWE 444
           P+L Y+ AC +E  RLHP  PFN+PHVS +D  VAGYFIPKGS V+LSRL LGRN R+WE
Sbjct: 380 PHLPYIRACAKEVMRLHPVGPFNLPHVSIADAEVAGYFIPKGSNVILSRLELGRNPRVWE 439

Query: 445 DLMRFNPERHLKNGT-IELGISEPDLRFVTFTRGRRGCIGTSLGTNMTMMLFARLVQGFS 471
           + +RFNPERHL      ++ + E DLRFV+F+ GRRGC+G  LG+ + +ML ARL+QGFS
Sbjct: 440 EPLRFNPERHLNIAVDQQVDLEENDLRFVSFSTGRRGCMGVGLGSTIVVMLLARLLQGFS 499

[Close]

BLAST of Lsi06G005660 vs. ExPASy Swiss-Prot
Match: Q6J541 (Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.7e-125
Identity = 233/517 (45.07%), Postives = 325/517 (62.86%), Query Frame = 0


Query: 23  TFIAIILFISSIIYVIRFSQYYCKRPLSIRRLPPGPKPWPLLGYLPAMLSNKNLPTHHWI 82
           TF+ +++F      + + ++ +        +LPPGPKPWP++G LP ML+N+  P   WI
Sbjct: 17  TFLLVVIF---SFMIFKVTKTHLVNKSKKYKLPPGPKPWPIVGNLPEMLANR--PATIWI 76

Query: 83  HSIMKQFNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFRSRPISMTAEIVSHGYLS 142
           H +MK+ NTEIACIRL +NT +IPVT P +A EFLK HD+ F SRP  M+ +I S G+++
Sbjct: 77  HKLMKEMNTEIACIRL-ANTIVIPVTCPTIACEFLKKHDASFASRPKIMSTDIASDGFIT 136

Query: 143 TVLSPMGDQWKKMRRILVSHVLNSSTLHQMLGQRTKEADILLCYIFN-----LSRNGEAI 202
           TVL P G+QWKKM+R+LV+++L+      +LG+R +EAD L+ YI+N     ++     +
Sbjct: 137 TVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKDVNDGPGLV 196

Query: 203 NIKSIVRHYCSNIVRRMLFNKRYYGKGREDGGPTFEEEDHNQALLSILRHVNAFSISEFI 262
           NI+   +HY  N+ R+++FN RY+GK  EDGGP FEE +H  A  +IL++V AFSIS+F+
Sbjct: 197 NIRIAAQHYGGNVFRKLIFNSRYFGKVMEDGGPGFEEVEHINATFTILKYVYAFSISDFV 256

Query: 263 PFLKLFDLDGHEK----------------------------------------------- 322
           PFL+  DLDGH                                                 
Sbjct: 257 PFLRRLDLDGHRSKIMKAMRIMRKYHDPIIDDRIKQWNDGLKTVEEDLLDVLIKLKDANN 316

Query: 323 -------------IDLQLASIDNPSNAVEWAMAELLNQPKLLQKAVEELDEVVGRDRLVQ 382
                        I+L +  +DNPSNA EWA+AE++NQP+LL++A EELD VVG++RLVQ
Sbjct: 317 KPLLTLKELKAQIIELAIEMVDNPSNAFEWALAEMINQPELLKRATEELDNVVGKERLVQ 376

Query: 383 EFDVPNLKYLTACIRESFRLHPFSPFNVPHVSNSDIVVAGYFIPKGSQVLLSRLGLGRNS 442
           E D+P L+++ AC RE+ RLHP   FNVPH+  +D +V  Y  PKG+QVLLSR+ LGRN 
Sbjct: 377 ESDIPKLQFVKACAREALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQVLLSRVALGRNP 436

Query: 443 RIWEDLMRFNPERHLKNGTIELGISEPDLRFVTFTRGRRGCIGTSLGTNMTMMLFARLVQ 473
           + W D ++FNPERHLK G I++ ++EPDLRF++FT GRR C G +LGT MT+MLFAR++ 
Sbjct: 437 KFWTDPLKFNPERHLKEG-IDVVLTEPDLRFISFTTGRRSCPGVALGTTMTVMLFARMLH 496

[Close]

BLAST of Lsi06G005660 vs. ExPASy Swiss-Prot
Match: Q6J540 (Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 3.7e-125
Identity = 233/517 (45.07%), Postives = 323/517 (62.48%), Query Frame = 0


Query: 23  TFIAIILFISSIIYVIRFSQYYCKRPLSIRRLPPGPKPWPLLGYLPAMLSNKNLPTHHWI 82
           TF+ +++F      + + ++ +        +LPPGPKPWP++G LP ML+N+  P   WI
Sbjct: 17  TFLLVVIF---FFIIFKVTKSHSVNKSKKYKLPPGPKPWPIVGNLPEMLANR--PATIWI 76

Query: 83  HSIMKQFNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFRSRPISMTAEIVSHGYLS 142
           H +MK+ NTEIACIRL +NT +IPVT P +A EFLK HD+ F SRP  M+ +I S G+L+
Sbjct: 77  HKLMKEMNTEIACIRL-ANTIVIPVTCPTIACEFLKKHDASFASRPKIMSTDIASDGFLT 136

Query: 143 TVLSPMGDQWKKMRRILVSHVLNSSTLHQMLGQRTKEADILLCYIFN-----LSRNGEAI 202
           TVL P G+QWKKM+R+LV+++L+      +LG+R +EAD L+ YI+N     ++     +
Sbjct: 137 TVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKDVNDGPGLV 196

Query: 203 NIKSIVRHYCSNIVRRMLFNKRYYGKGREDGGPTFEEEDHNQALLSILRHVNAFSISEFI 262
           NI+   +HY  N+ R+++FN RY+GK  EDGGP FEE +H  A  +IL++V AFSIS+FI
Sbjct: 197 NIRIAAQHYGGNVFRKLIFNTRYFGKVMEDGGPGFEEVEHINATFTILKYVYAFSISDFI 256

Query: 263 PFLKLFDLDGHEK----------------------------------------------- 322
           PFL+  DLDGH                                                 
Sbjct: 257 PFLRRLDLDGHRSKIMKAMGIMKKYHDPIIHDRIKQWNDGLKTVEEDLLDVLIKLKDASN 316

Query: 323 -------------IDLQLASIDNPSNAVEWAMAELLNQPKLLQKAVEELDEVVGRDRLVQ 382
                         +L +  +DNPSNA EWA+AE+LNQP+LL++A EELD VVG++RLVQ
Sbjct: 317 KPLLTLKEIKAQITELAIEMVDNPSNAFEWALAEMLNQPELLKRATEELDNVVGKERLVQ 376

Query: 383 EFDVPNLKYLTACIRESFRLHPFSPFNVPHVSNSDIVVAGYFIPKGSQVLLSRLGLGRNS 442
           E D+P L+++ AC RE+ RLHP   FNVPH+  +D +V  Y  PKG+QVLLSR+ LGRN 
Sbjct: 377 ESDIPKLQFVKACAREALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQVLLSRVALGRNP 436

Query: 443 RIWEDLMRFNPERHLKNGTIELGISEPDLRFVTFTRGRRGCIGTSLGTNMTMMLFARLVQ 473
           + W D ++FNPERHLK G I++ ++EPDLRF++FT GRR C G +LGT MT+MLFAR++ 
Sbjct: 437 KFWTDPLKFNPERHLKEG-IDVVLTEPDLRFISFTTGRRSCPGVTLGTTMTIMLFARMLH 496

[Close]

BLAST of Lsi06G005660 vs. ExPASy Swiss-Prot
Match: Q9FLC8 (Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 6.4e-125
Identity = 226/487 (46.41%), Postives = 311/487 (63.86%), Query Frame = 0


Query: 54  LPPGPKPWPLLGYLPAMLSNKNLPTHHWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELA 113
           LPPGPK WPL+G LP +L  +N P   WIHS+MK+ NT+IACIRL +NTH+IPVTSP +A
Sbjct: 40  LPPGPKSWPLIGNLPEIL-GRNKPVFRWIHSLMKELNTDIACIRL-ANTHVIPVTSPRIA 99

Query: 114 LEFLKTHDSVFRSRPISMTAEIVSHGYLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQML 173
            E LK  DSVF +RP++M  E  S GYL+  + P G+QWKKMRR++ SHV +  +   ML
Sbjct: 100 REILKKQDSVFATRPLTMGTEYCSRGYLTVAVEPQGEQWKKMRRVVASHVTSKKSFQMML 159

Query: 174 GQRTKEADILLCYIFNLS--RNGEA---INIKSIVRHYCSNIVRRMLFNKRYYGKGREDG 233
            +RT+EAD L+ YI N S    G A   I+++  VR Y  N+ R+M+F  R++GKG EDG
Sbjct: 160 QKRTEEADNLVRYINNRSVKNRGNAFVVIDLRLAVRQYSGNVARKMMFGIRHFGKGSEDG 219

Query: 234 -GPTFEEEDHNQALLSILRHVNAFSISEFIPFLKLFDLDGHEKI---------------- 293
            GP  EE +H ++L ++L H+ AF++S+++P+L+  DL+GHEK+                
Sbjct: 220 SGPGLEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVSNAMRNVSKYNDPFV 279

Query: 294 --------------------------------------------DLQLASIDNPSNAVEW 353
                                                       +L LA++DNPSNA EW
Sbjct: 280 DERLMQWRNGKMKEPQDFLDMFIIAKDTDGKPTLSDEEIKAQVTELMLATVDNPSNAAEW 339

Query: 354 AMAELLNQPKLLQKAVEELDEVVGRDRLVQEFDVPNLKYLTACIRESFRLHPFSPFNVPH 413
            MAE++N+P ++QKAVEE+D VVG+DRLV E D+PNL Y+ AC++E+FRLHP +PFN+PH
Sbjct: 340 GMAEMINEPSIMQKAVEEIDRVVGKDRLVIESDLPNLNYVKACVKEAFRLHPVAPFNLPH 399

Query: 414 VSNSDIVVAGYFIPKGSQVLLSRLGLGRNSRIWEDLMRFNPERHLKNGTIELGISEPDLR 473
           +S +D VV GYFIPKGS VL+SR+G+GRN  +W+   +F+PERHL   T  + ++E DL 
Sbjct: 400 MSTTDTVVDGYFIPKGSHVLISRMGIGRNPSVWDKPHKFDPERHLSTNTC-VDLNESDLN 459

[Close]

BLAST of Lsi06G005660 vs. ExPASy Swiss-Prot
Match: Q43135 (Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3)

HSP 1 Score: 443.4 bits (1139), Expect = 3.5e-123
Identity = 218/483 (45.13%), Postives = 306/483 (63.35%), Query Frame = 0


Query: 54  LPPGPKPWPLLGYLPAMLSNKNLPTHHWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELA 113
           LPPGP PWP++G LP ML NK  P   WIH +M++  T+IAC++LG   H++ +T PE+A
Sbjct: 68  LPPGPVPWPVVGNLPEMLLNK--PAFRWIHQMMREMGTDIACVKLG-GVHVVSITCPEIA 127

Query: 114 LEFLKTHDSVFRSRPISMTAEIVSHGYLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQML 173
            E L+  D+ F SRP++  +E  S GY + VLSP GDQWKKMRR+L S ++  S    + 
Sbjct: 128 REVLRKQDANFISRPLTFASETFSGGYRNAVLSPYGDQWKKMRRVLTSEIICPSRHAWLH 187

Query: 174 GQRTKEADILLCYIFNLSRNGE----AINIKSIVRHYCSNIVRRMLFNKRYYGKGREDGG 233
            +RT EAD L  Y++NL+        A++++ + RHYC N++RR++FN+RY+G+ + DGG
Sbjct: 188 DKRTDEADNLTRYVYNLATKAATGDVAVDVRHVARHYCGNVIRRLMFNRRYFGEPQADGG 247

Query: 234 PTFEEEDHNQALLSILRHVNAFSISEFIPFLKLFDLDGHEKI------------------ 293
           P   E  H  A+ + L  + AF +S+++P+L+  DLDGHEKI                  
Sbjct: 248 PGPMEVLHMDAVFTSLGLLYAFCVSDYLPWLRGLDLDGHEKIVKEANVAVNRLHDTVIDD 307

Query: 294 ------------------------------------------DLQLASIDNPSNAVEWAM 353
                                                     D+  A++DNPSNAVEWA+
Sbjct: 308 RWRQWKSGERQEMEDFLDVLITLKDAQGNPLLTIEEVKAQSQDITFAAVDNPSNAVEWAL 367

Query: 354 AELLNQPKLLQKAVEELDEVVGRDRLVQEFDVPNLKYLTACIRESFRLHPFSPFNVPHVS 413
           AE++N P+++ KA+EELD VVGR+RLVQE D+P L Y+ ACIRE+FRLHP +PFNVPHV+
Sbjct: 368 AEMVNNPEVMAKAMEELDRVVGRERLVQESDIPKLNYVKACIREAFRLHPVAPFNVPHVA 427

Query: 414 NSDIVVAGYFIPKGSQVLLSRLGLGRNSRIWEDLMRFNPERHLKNGTIELGISEPDLRFV 471
            +D  +AGY +PKGS V+LSR GLGRN R+W++ +RF P+RHL     ++ ++E DLRF+
Sbjct: 428 LADTTIAGYRVPKGSHVILSRTGLGRNPRVWDEPLRFYPDRHLATAASDVALTENDLRFI 487

[Close]

BLAST of Lsi06G005660 vs. ExPASy TrEMBL
Match: A0A5A7VKT9 (Isoleucine N-monooxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold14G00680 PE=3 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 3.3e-209
Identity = 376/516 (72.87%), Postives = 414/516 (80.23%), Query Frame = 0


Query: 20  IAITFIAIILFISSIIYVIRFSQYYCKRPLSIRRLPPGPKPWPLLGYLPAMLSNKNLPTH 79
           +AITFIAIILFI SI+Y I+FSQYY     SIRRLPPGPKPWPL+G LPAML NKNLPT+
Sbjct: 1   MAITFIAIILFIFSILYAIKFSQYYHNGTPSIRRLPPGPKPWPLIGCLPAMLRNKNLPTY 60

Query: 80  HWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFRSRPISMTAEIVSHG 139
            WIH +MKQFNTEIACIRLGSNTHIIPV SPEL+LEFL THDSVF SR ISMTAEIVS+G
Sbjct: 61  QWIHEVMKQFNTEIACIRLGSNTHIIPVASPELSLEFLNTHDSVFGSRSISMTAEIVSNG 120

Query: 140 YLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQMLGQRTKEADILLCYIFNLSRNGEAINI 199
           YLSTVLSPMG+QWKKMR+ILVS VLN  TLHQM+GQRT EADILL YIF L+RNGEAINI
Sbjct: 121 YLSTVLSPMGEQWKKMRKILVSQVLNPPTLHQMVGQRTDEADILLRYIFGLTRNGEAINI 180

Query: 200 KSIVRHYCSNIVRRMLFNKRYYGKGREDGGPTFEEEDHNQALLSILRHVNAFSISEFIPF 259
           +SIVRHYC  ++RRM+F++RYYGKGREDGGP+ EEEDHNQALLSILRHVNAFSISEFIP 
Sbjct: 181 RSIVRHYCGTVIRRMIFSRRYYGKGREDGGPSLEEEDHNQALLSILRHVNAFSISEFIPL 240

Query: 260 LKLFDLDGHEKI------------------------------------------------ 319
           LK FDLDGHEKI                                                
Sbjct: 241 LKKFDLDGHEKIVKRALKVIRNNDEPIIEERVQDWRDGKKKKAEDILDILISLKNENGKS 300

Query: 320 ------------DLQLASIDNPSNAVEWAMAELLNQPKLLQKAVEELDEVVGRDRLVQEF 379
                       DLQLASIDNPSNAVEWA+AELLNQPK++QKAVEELD+V+GRDRLVQE 
Sbjct: 301 LLSIEEIKAQVTDLQLASIDNPSNAVEWALAELLNQPKIIQKAVEELDKVIGRDRLVQEC 360

Query: 380 DVPNLKYLTACIRESFRLHPFSPFNVPHVSNSDIVVAGYFIPKGSQVLLSRLGLGRNSRI 439
           D+PNL YLTACIRESFRLHPFSPFNVPHVSNSD+ VAGYFIP+GSQVLLSRLGLGRN RI
Sbjct: 361 DIPNLMYLTACIRESFRLHPFSPFNVPHVSNSDVFVAGYFIPRGSQVLLSRLGLGRNPRI 420

Query: 440 WEDLMRFNPERHLKNGTIELGISEPDLRFVTFTRGRRGCIGTSLGTNMTMMLFARLVQGF 474
           WED MRF+PERHLK+GTI+ GISEP LRF+TFTRGRRGCIGTSLGTN+TMMLFARL+QGF
Sbjct: 421 WEDPMRFDPERHLKDGTIKFGISEPSLRFITFTRGRRGCIGTSLGTNITMMLFARLLQGF 480

[Close]

BLAST of Lsi06G005660 vs. ExPASy TrEMBL
Match: A0A1S3BPW4 (phenylalanine N-monooxygenase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491937 PE=3 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 3.0e-194
Identity = 358/537 (66.67%), Postives = 410/537 (76.35%), Query Frame = 0


Query: 1   MKVLLFISESSYQNYAKESIAITFIAIILFISS-IIYVIRFSQYYCKRPLSIRRLPPGPK 60
           MK LLF S+ + QN+ K +I  TFI+I+LFISS I+Y+IR SQYY KR     RLPPGP+
Sbjct: 1   MKALLFNSQLACQNFEKPAIPKTFISILLFISSLIVYLIRISQYYNKRNPLEPRLPPGPR 60

Query: 61  PWPLLG-YLPAMLSN-KNLPTHHWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELALEFL 120
           PWPL G  LP MLS+ K  P H WIH++MKQ NTEIAC  LGSNTHII VTSPELALEFL
Sbjct: 61  PWPLFGSCLPLMLSSYKRSPRHQWIHTVMKQLNTEIACFHLGSNTHIIIVTSPELALEFL 120

Query: 121 KTHDSVFRSRPISMTAEIVSHGYLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQMLGQRT 180
           KTHDS F SRPI+ T EI+S G+LSTVLSPMGDQWKKM+RIL SHVLN STLHQM GQR 
Sbjct: 121 KTHDSTFASRPIASTTEILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRI 180

Query: 181 KEADILLCYIFNLSRNGEAINIKSIVRHYCSNIVRRMLFNKRYYGKGREDGGPTFEEEDH 240
           KEAD LL YIF+ +   EAINI+SI +HYCSNI+RRM+FN+RYY KGRE+ GP+ EEED+
Sbjct: 181 KEADALLHYIFDHASRCEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDY 240

Query: 241 NQALLSILRHVNAFSISEFIPFLKLFDLDGHEKI-------------------------- 300
           NQALL+IL H+ +FSISE+I  LK FDL GH+KI                          
Sbjct: 241 NQALLTILMHLYSFSISEYIACLKPFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDG 300

Query: 301 ----------------------------------DLQLASIDNPSNAVEWAMAELLNQPK 360
                                             +LQLA+IDNPSNAVEWAM ELLNQPK
Sbjct: 301 KKKEVQDILDILITVKKENGESLLSIEEIKAQVTELQLATIDNPSNAVEWAMIELLNQPK 360

Query: 361 LLQKAVEELDEVVGRDRLVQEFDVPNLKYLTACIRESFRLHPFSPFNVPHVSNSDIVVAG 420
           +LQK VEELDEVVGRDRLVQEFD+PNLKYLTAC+RESFRLHPFSPFN+PHVS SDIVV G
Sbjct: 361 ILQKVVEELDEVVGRDRLVQEFDIPNLKYLTACMRESFRLHPFSPFNLPHVSISDIVVGG 420

Query: 421 YFIPKGSQVLLSRLGLGRNSRIWEDLMRFNPERHLKNGTIELGISEPDLRFVTFTRGRRG 474
           YFIPKGSQ+LL R+GLGRN RIWED MRF+PERHL++ T++ G+SEP LRF+TFTRGRRG
Sbjct: 421 YFIPKGSQILLCRVGLGRNPRIWEDPMRFDPERHLRDETVKFGLSEPSLRFITFTRGRRG 480

[Close]

BLAST of Lsi06G005660 vs. ExPASy TrEMBL
Match: A0A6J1D6G9 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498 PE=3 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 4.5e-182
Identity = 336/543 (61.88%), Postives = 402/543 (74.03%), Query Frame = 0


Query: 1   MKVLLFISESSYQNYAKESIAITFIAIIL--FISSIIYVIRFSQYYCKRPLSIRRLPPGP 60
           M+ LLFIS+ +YQN +  +I  TF   +   FIS+I     FSQ Y KR  S+ +LPPGP
Sbjct: 1   MEALLFISQLTYQN-SPTAITKTFTLFVFMAFISAI-----FSQCYRKRKHSVLQLPPGP 60

Query: 61  KPWPLLGYLPAMLSNKNLPTHHWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELALEFLK 120
            PWPL+G LPAML+ K+ P + WIH++MKQF+TEIACIRLG NT+++PVTSPELA+EFLK
Sbjct: 61  NPWPLVGCLPAMLATKS-PKYKWIHAVMKQFDTEIACIRLG-NTYVMPVTSPELAVEFLK 120

Query: 121 THDSVFRSR-PISMTAEIVSHGYLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQMLGQRT 180
            HDSVF SR  IS T  ++S G L+T  SP+G QWKKMRRIL S +   STLHQMLG RT
Sbjct: 121 RHDSVFASRSTISNTVNVLSRGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRT 180

Query: 181 KEADILLCYIFNL------SRNGEAINIKSIVRHYCSNIVRRMLFNKRYYGKGREDGGPT 240
            EAD LL YIF+L      +++G  +N++S+ +HYC NI+RRMLFNKRYYGKGREDGGP+
Sbjct: 181 NEADTLLRYIFSLTTTNTVNKDGAVVNVRSVTQHYCGNIIRRMLFNKRYYGKGREDGGPS 240

Query: 241 FEEEDHNQALLSILRHVNAFSISEFIPFLKLFDLDGHEKI-------------------- 300
           FEEE+HNQALL+ILRHVNAFSIS+F+P LK FDLDGHEKI                    
Sbjct: 241 FEEEEHNQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKRALKALWKYDEPIINERV 300

Query: 301 ----------------------------------------DLQLASIDNPSNAVEWAMAE 360
                                                   +LQ+A+IDNPSNAVEW MAE
Sbjct: 301 QQWKDGKREGAEDILDILISTKDGNGKSLLTIDEIKAQITELQIATIDNPSNAVEWTMAE 360

Query: 361 LLNQPKLLQKAVEELDEVVGRDRLVQEFDVPNLKYLTACIRESFRLHPFSPFNVPHVSNS 420
           LLNQPK+LQKA+EELD VVGR+RLVQE D+P LKYLTAC RESFRLHPFSPFN+PHVSN+
Sbjct: 361 LLNQPKILQKAIEELDRVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNIPHVSNA 420

Query: 421 DIVVAGYFIPKGSQVLLSRLGLGRNSRIWEDLMRFNPERHLKNGTIELGISEPDLRFVTF 474
           DIVVAGYFIPKGS+V+LSR GLGRN RIWED MRF+PERHLK+ T+ELG+SEP+LRF++F
Sbjct: 421 DIVVAGYFIPKGSEVILSRSGLGRNPRIWEDPMRFDPERHLKDETVELGLSEPNLRFISF 480

[Close]

BLAST of Lsi06G005660 vs. ExPASy TrEMBL
Match: A0A1S3BNA6 (phenylalanine N-monooxygenase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491937 PE=3 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 5.9e-182
Identity = 336/511 (65.75%), Postives = 386/511 (75.54%), Query Frame = 0


Query: 1   MKVLLFISESSYQNYAKESIAITFIAIILFISS-IIYVIRFSQYYCKRPLSIRRLPPGPK 60
           MK LLF S+ + QN+ K +I  TFI+I+LFISS I+Y+IR SQYY KR     RLPPGP+
Sbjct: 1   MKALLFNSQLACQNFEKPAIPKTFISILLFISSLIVYLIRISQYYNKRNPLEPRLPPGPR 60

Query: 61  PWPLLG-YLPAMLSN-KNLPTHHWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELALEFL 120
           PWPL G  LP MLS+ K  P H WIH++MKQ NTEIAC  LGSNTHII VTSPELALEFL
Sbjct: 61  PWPLFGSCLPLMLSSYKRSPRHQWIHTVMKQLNTEIACFHLGSNTHIIIVTSPELALEFL 120

Query: 121 KTHDSVFRSRPISMTAEIVSHGYLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQMLGQRT 180
           KTHDS F SRPI+ T EI+S G+LSTVLSPMGDQWKKM+RIL SHVLN STLHQM GQR 
Sbjct: 121 KTHDSTFASRPIASTTEILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRI 180

Query: 181 KEADILLCYIFNLSRNGEAINIKSIVRHYCSNIVRRMLFNKRYYGKGREDGGPTFEEEDH 240
           KEAD LL YIF+ +   EAINI+SI +HYCSNI+RRM+FN+RYY KGRE+ GP+ EEED+
Sbjct: 181 KEADALLHYIFDHASRCEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDY 240

Query: 241 NQALLSILRHVNAFSISEFIPFLKLFDLDGHEKI-------------------------- 300
           NQALL+IL H+ +FSISE+I  LK FDL GH+KI                          
Sbjct: 241 NQALLTILMHLYSFSISEYIACLKPFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDG 300

Query: 301 ----------------------------------DLQLASIDNPSNAVEWAMAELLNQPK 360
                                             +LQLA+IDNPSNAVEWAM ELLNQPK
Sbjct: 301 KKKEVQDILDILITVKKENGESLLSIEEIKAQVTELQLATIDNPSNAVEWAMIELLNQPK 360

Query: 361 LLQKAVEELDEVVGRDRLVQEFDVPNLKYLTACIRESFRLHPFSPFNVPHVSNSDIVVAG 420
           +LQK VEELDEVVGRDRLVQEFD+PNLKYLTAC+RESFRLHPFSPFN+PHVS SDIVV G
Sbjct: 361 ILQKVVEELDEVVGRDRLVQEFDIPNLKYLTACMRESFRLHPFSPFNLPHVSISDIVVGG 420

Query: 421 YFIPKGSQVLLSRLGLGRNSRIWEDLMRFNPERHLKNGTIELGISEPDLRFVTFTRGRRG 449
           YFIPKGSQ+LL R+GLGRN RIWED MRF+PERHL++ T++ G+SEP LRF+TFTRGRRG
Sbjct: 421 YFIPKGSQILLCRVGLGRNPRIWEDPMRFDPERHLRDETVKFGLSEPSLRFITFTRGRRG 480

[Close]

BLAST of Lsi06G005660 vs. ExPASy TrEMBL
Match: A0A6J1H9V7 (isoleucine N-monooxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461429 PE=3 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 1.6e-179
Identity = 325/514 (63.23%), Postives = 387/514 (75.29%), Query Frame = 0


Query: 28  ILFISSIIYVIRFSQYYCKRPLS---IRRLPPGPKPWPLLGYLPAMLSNKNLPTHHWIHS 87
           +L     I+V+    YY KR  S      LPPGPKPWPL+G LP ML+  NLPT+ WIH+
Sbjct: 6   VLLFMPFIFVMFSQCYYRKRKHSNGEAPELPPGPKPWPLVGCLPTMLTT-NLPTYQWIHA 65

Query: 88  IMKQFNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFRSR-PISMTAEIVSHGYLST 147
           +MKQFN EIACIRLGS  +IIPV SP+LALEFLKT+DS+F SR  IS T + +SH YL+ 
Sbjct: 66  VMKQFNAEIACIRLGS-IYIIPVASPQLALEFLKTYDSIFASRSSISNTVDTLSHRYLTA 125

Query: 148 VLSPMGDQWKKMRRILVSHVLNSSTLHQMLGQRTKEADILLCYIFN--LSRNGEAINIKS 207
             SP+G QW+KMRRIL S +LNSSTLH+MLG R  EAD LL YIF+   +R+G  +N++S
Sbjct: 126 AFSPLGKQWRKMRRILASEILNSSTLHRMLGHRNAEADTLLRYIFSQTSNRDGAVVNLRS 185

Query: 208 IVRHYCSNIVRRMLFNKRYYGKGREDGGPTFEEEDHNQALLSILRHVNAFSISEFIPFLK 267
           I +HYC NI+RRMLFNKRYYGKGREDGGPTFEEE+HNQALL+IL+HVNAFS S+F+P+LK
Sbjct: 186 ITQHYCGNIIRRMLFNKRYYGKGREDGGPTFEEEEHNQALLTILKHVNAFSTSDFMPWLK 245

Query: 268 LFDLDGHEKI-------------------------------------------------- 327
            FDLDGH+KI                                                  
Sbjct: 246 PFDLDGHQKIMKNALNVVQKYDEPIIDERVQRWKDGKMEGVKEDILNILISLKDENAKPL 305

Query: 328 -----------DLQLASIDNPSNAVEWAMAELLNQPKLLQKAVEELDEVVGRDRLVQEFD 387
                      +LQ+A+IDNPSNAVEWAMAELLNQPK+L+KA+EELD+VVGR+RLVQE+D
Sbjct: 306 LSVAEIKAQITELQIATIDNPSNAVEWAMAELLNQPKILEKAIEELDKVVGRERLVQEYD 365

Query: 388 VPNLKYLTACIRESFRLHPFSPFNVPHVSNSDIVVAGYFIPKGSQVLLSRLGLGRNSRIW 447
           +PNLKYLTACIRESFRLHPFSPFNVPHVSN+DI+VAGYFIPKGS+VLLSR+ LGRN RIW
Sbjct: 366 IPNLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRISLGRNPRIW 425

Query: 448 EDLMRFNPERHLKNGTIELGISEPDLRFVTFTRGRRGCIGTSLGTNMTMMLFARLVQGFS 474
           ED MRFNPERHL + T+ELG+ EP+LRF++F+RGRRGC G+SLGTN+TMMLFARL+QGFS
Sbjct: 426 EDPMRFNPERHLNDRTVELGLLEPNLRFMSFSRGRRGCPGSSLGTNITMMLFARLLQGFS 485

[Close]

BLAST of Lsi06G005660 vs. NCBI nr
Match: XP_011655052.1 (isoleucine N-monooxygenase 1 [Cucumis sativus] >KAE8648059.1 hypothetical protein Csa_005885 [Cucumis sativus])

HSP 1 Score: 746.9 bits (1927), Expect = 1.1e-211
Identity = 376/515 (73.01%), Postives = 416/515 (80.78%), Query Frame = 0


Query: 20  IAITFIAIILFISSIIYVIRFSQYYCKRPLSIRRLPPGPKPWPLLGYLPAMLSNKNLPTH 79
           +AITF+AIILFI SIIY I+FSQYY K   SIRRLPPGPKPWPL+G LPAMLSNKNLP +
Sbjct: 1   MAITFLAIILFIYSIIYAIKFSQYYLKGTPSIRRLPPGPKPWPLIGCLPAMLSNKNLPAY 60

Query: 80  HWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFRSRPISMTAEIVSHG 139
            WIH +MKQFNTEIACIRLGSNTHIIPV SPEL+LEFL THDSVF SR ISMTAEIVS+G
Sbjct: 61  QWIHEVMKQFNTEIACIRLGSNTHIIPVASPELSLEFLNTHDSVFGSRSISMTAEIVSNG 120

Query: 140 YLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQMLGQRTKEADILLCYIFNLSRNGEAINI 199
           YLSTVLSPMG+QWKKMR+IL S VLNSSTLH+MLGQRT EADILL YIF L++NGEAINI
Sbjct: 121 YLSTVLSPMGEQWKKMRKILASQVLNSSTLHRMLGQRTDEADILLRYIFGLTKNGEAINI 180

Query: 200 KSIVRHYCSNIVRRMLFNKRYYGKGREDGGPTFEEEDHNQALLSILRHVNAFSISEFIPF 259
           +SIVRHYC  ++RRM+F++RYYGKGREDGGP+ EEE+HNQALLSILRHVNAFSISEFIP 
Sbjct: 181 RSIVRHYCGTVIRRMIFSRRYYGKGREDGGPSLEEEEHNQALLSILRHVNAFSISEFIPL 240

Query: 260 LKLFDLDGHEKI------------------------------------------------ 319
           LK FDLDGH KI                                                
Sbjct: 241 LKTFDLDGHGKIVKRALKVIRNHDEPIIEERVQEWGDGKKKKVEDILDILISLKNENGKS 300

Query: 320 ------------DLQLASIDNPSNAVEWAMAELLNQPKLLQKAVEELDEVVGRDRLVQEF 379
                       DLQLASIDNPSNAVEWA+AELLNQPK++Q+A+EELD+V+GRDRLVQE 
Sbjct: 301 LLSIEEIKAQVTDLQLASIDNPSNAVEWALAELLNQPKIIQQALEELDKVIGRDRLVQES 360

Query: 380 DVPNLKYLTACIRESFRLHPFSPFNVPHVSNSDIVVAGYFIPKGSQVLLSRLGLGRNSRI 439
           D+PNLKYLTACIRESFRLHPFSPFNVPHVSNSD+ VAGYFIPKGSQVLLSRLGLGRN RI
Sbjct: 361 DIPNLKYLTACIRESFRLHPFSPFNVPHVSNSDVFVAGYFIPKGSQVLLSRLGLGRNPRI 420

Query: 440 WEDLMRFNPERHLKNGTIELGISEPDLRFVTFTRGRRGCIGTSLGTNMTMMLFARLVQGF 474
           WED M+F+PERHLK+GT++ GISEP LRF+TFTRGRRGCIGTSLGTN+TMMLFARL+QGF
Sbjct: 421 WEDPMKFDPERHLKDGTVKFGISEPSLRFITFTRGRRGCIGTSLGTNITMMLFARLLQGF 480

[Close]

BLAST of Lsi06G005660 vs. NCBI nr
Match: KAA0067056.1 (isoleucine N-monooxygenase 1 [Cucumis melo var. makuwa] >TYK26293.1 isoleucine N-monooxygenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 737.6 bits (1903), Expect = 6.8e-209
Identity = 376/516 (72.87%), Postives = 414/516 (80.23%), Query Frame = 0


Query: 20  IAITFIAIILFISSIIYVIRFSQYYCKRPLSIRRLPPGPKPWPLLGYLPAMLSNKNLPTH 79
           +AITFIAIILFI SI+Y I+FSQYY     SIRRLPPGPKPWPL+G LPAML NKNLPT+
Sbjct: 1   MAITFIAIILFIFSILYAIKFSQYYHNGTPSIRRLPPGPKPWPLIGCLPAMLRNKNLPTY 60

Query: 80  HWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFRSRPISMTAEIVSHG 139
            WIH +MKQFNTEIACIRLGSNTHIIPV SPEL+LEFL THDSVF SR ISMTAEIVS+G
Sbjct: 61  QWIHEVMKQFNTEIACIRLGSNTHIIPVASPELSLEFLNTHDSVFGSRSISMTAEIVSNG 120

Query: 140 YLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQMLGQRTKEADILLCYIFNLSRNGEAINI 199
           YLSTVLSPMG+QWKKMR+ILVS VLN  TLHQM+GQRT EADILL YIF L+RNGEAINI
Sbjct: 121 YLSTVLSPMGEQWKKMRKILVSQVLNPPTLHQMVGQRTDEADILLRYIFGLTRNGEAINI 180

Query: 200 KSIVRHYCSNIVRRMLFNKRYYGKGREDGGPTFEEEDHNQALLSILRHVNAFSISEFIPF 259
           +SIVRHYC  ++RRM+F++RYYGKGREDGGP+ EEEDHNQALLSILRHVNAFSISEFIP 
Sbjct: 181 RSIVRHYCGTVIRRMIFSRRYYGKGREDGGPSLEEEDHNQALLSILRHVNAFSISEFIPL 240

Query: 260 LKLFDLDGHEKI------------------------------------------------ 319
           LK FDLDGHEKI                                                
Sbjct: 241 LKKFDLDGHEKIVKRALKVIRNNDEPIIEERVQDWRDGKKKKAEDILDILISLKNENGKS 300

Query: 320 ------------DLQLASIDNPSNAVEWAMAELLNQPKLLQKAVEELDEVVGRDRLVQEF 379
                       DLQLASIDNPSNAVEWA+AELLNQPK++QKAVEELD+V+GRDRLVQE 
Sbjct: 301 LLSIEEIKAQVTDLQLASIDNPSNAVEWALAELLNQPKIIQKAVEELDKVIGRDRLVQEC 360

Query: 380 DVPNLKYLTACIRESFRLHPFSPFNVPHVSNSDIVVAGYFIPKGSQVLLSRLGLGRNSRI 439
           D+PNL YLTACIRESFRLHPFSPFNVPHVSNSD+ VAGYFIP+GSQVLLSRLGLGRN RI
Sbjct: 361 DIPNLMYLTACIRESFRLHPFSPFNVPHVSNSDVFVAGYFIPRGSQVLLSRLGLGRNPRI 420

Query: 440 WEDLMRFNPERHLKNGTIELGISEPDLRFVTFTRGRRGCIGTSLGTNMTMMLFARLVQGF 474
           WED MRF+PERHLK+GTI+ GISEP LRF+TFTRGRRGCIGTSLGTN+TMMLFARL+QGF
Sbjct: 421 WEDPMRFDPERHLKDGTIKFGISEPSLRFITFTRGRRGCIGTSLGTNITMMLFARLLQGF 480

[Close]

BLAST of Lsi06G005660 vs. NCBI nr
Match: XP_008450287.1 (PREDICTED: phenylalanine N-monooxygenase-like isoform X1 [Cucumis melo])

HSP 1 Score: 688.0 bits (1774), Expect = 6.2e-194
Identity = 358/537 (66.67%), Postives = 410/537 (76.35%), Query Frame = 0


Query: 1   MKVLLFISESSYQNYAKESIAITFIAIILFISS-IIYVIRFSQYYCKRPLSIRRLPPGPK 60
           MK LLF S+ + QN+ K +I  TFI+I+LFISS I+Y+IR SQYY KR     RLPPGP+
Sbjct: 1   MKALLFNSQLACQNFEKPAIPKTFISILLFISSLIVYLIRISQYYNKRNPLEPRLPPGPR 60

Query: 61  PWPLLG-YLPAMLSN-KNLPTHHWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELALEFL 120
           PWPL G  LP MLS+ K  P H WIH++MKQ NTEIAC  LGSNTHII VTSPELALEFL
Sbjct: 61  PWPLFGSCLPLMLSSYKRSPRHQWIHTVMKQLNTEIACFHLGSNTHIIIVTSPELALEFL 120

Query: 121 KTHDSVFRSRPISMTAEIVSHGYLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQMLGQRT 180
           KTHDS F SRPI+ T EI+S G+LSTVLSPMGDQWKKM+RIL SHVLN STLHQM GQR 
Sbjct: 121 KTHDSTFASRPIASTTEILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRI 180

Query: 181 KEADILLCYIFNLSRNGEAINIKSIVRHYCSNIVRRMLFNKRYYGKGREDGGPTFEEEDH 240
           KEAD LL YIF+ +   EAINI+SI +HYCSNI+RRM+FN+RYY KGRE+ GP+ EEED+
Sbjct: 181 KEADALLHYIFDHASRCEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDY 240

Query: 241 NQALLSILRHVNAFSISEFIPFLKLFDLDGHEKI-------------------------- 300
           NQALL+IL H+ +FSISE+I  LK FDL GH+KI                          
Sbjct: 241 NQALLTILMHLYSFSISEYIACLKPFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDG 300

Query: 301 ----------------------------------DLQLASIDNPSNAVEWAMAELLNQPK 360
                                             +LQLA+IDNPSNAVEWAM ELLNQPK
Sbjct: 301 KKKEVQDILDILITVKKENGESLLSIEEIKAQVTELQLATIDNPSNAVEWAMIELLNQPK 360

Query: 361 LLQKAVEELDEVVGRDRLVQEFDVPNLKYLTACIRESFRLHPFSPFNVPHVSNSDIVVAG 420
           +LQK VEELDEVVGRDRLVQEFD+PNLKYLTAC+RESFRLHPFSPFN+PHVS SDIVV G
Sbjct: 361 ILQKVVEELDEVVGRDRLVQEFDIPNLKYLTACMRESFRLHPFSPFNLPHVSISDIVVGG 420

Query: 421 YFIPKGSQVLLSRLGLGRNSRIWEDLMRFNPERHLKNGTIELGISEPDLRFVTFTRGRRG 474
           YFIPKGSQ+LL R+GLGRN RIWED MRF+PERHL++ T++ G+SEP LRF+TFTRGRRG
Sbjct: 421 YFIPKGSQILLCRVGLGRNPRIWEDPMRFDPERHLRDETVKFGLSEPSLRFITFTRGRRG 480

[Close]

BLAST of Lsi06G005660 vs. NCBI nr
Match: XP_038880438.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 649.0 bits (1673), Expect = 3.2e-182
Identity = 341/538 (63.38%), Postives = 404/538 (75.09%), Query Frame = 0


Query: 5   LFISESSYQNYAKESIAITFIAIILFISSIIYVIRFSQYYC--KRPLSIRR--LPPGPKP 64
           LFI + ++QN     IAIT   +I+F    +  I FSQ YC  +R   +RR  LPPGPKP
Sbjct: 6   LFIPQLAHQN---SPIAITSKTLIVFGFMALTSILFSQCYCNRRRKHLVRRPPLPPGPKP 65

Query: 65  WPLLGYLPAMLSNKNLPTHHWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELALEFLKTH 124
           WPL+G LP ML NK+ PTH WIHS+MK+ NTEIA IRLG NT++IPVTSPELALEFLKT+
Sbjct: 66  WPLVGCLPTMLINKS-PTHQWIHSVMKKLNTEIASIRLG-NTYVIPVTSPELALEFLKTY 125

Query: 125 DSVFRSRPISMTA-EIVSHGYLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQMLGQRTKE 184
           DSVF SR  +  A  I++ GY+ST+LSP+GDQWKKMRRIL S +LN STLH+MLGQRT E
Sbjct: 126 DSVFASRSSTSNAIYILTRGYVSTILSPLGDQWKKMRRILTSKILNPSTLHRMLGQRTAE 185

Query: 185 ADILLCYIF---NLSRNGEAINIKSIVRHYCSNIVRRMLFNKRYYGKGREDGGPTFEEED 244
           AD LL YIF   N +R G  IN++SI +HYC NI+R+MLFN+RYYGKGREDGGPTFEEE+
Sbjct: 186 ADTLLRYIFTQTNNNRGGAVINLRSITQHYCGNILRQMLFNRRYYGKGREDGGPTFEEEE 245

Query: 245 HNQALLSILRHVNAFSISEFIPFLKLFDLDGHEKI------------------------- 304
           H QALL+I++H++AFSIS+F+P LK FDLDGH+KI                         
Sbjct: 246 HVQALLTIVKHIHAFSISDFMPCLKSFDLDGHQKIMKNALNILQKYDEPIIDERVQEWKD 305

Query: 305 -----------------------------------DLQLASIDNPSNAVEWAMAELLNQP 364
                                              DLQ+A+IDNPSNAVEWAMAELLNQP
Sbjct: 306 KKIKEVEDILDILISLKDENTNSLLSIEEIKAQITDLQIATIDNPSNAVEWAMAELLNQP 365

Query: 365 KLLQKAVEELDEVVGRDRLVQEFDVPNLKYLTACIRESFRLHPFSPFNVPHVSNSDIVVA 424
           K+L+K VEELD+VVGR+RLVQE D+P LKYLTAC RESFRLHPFS FNVPH+S SDIVVA
Sbjct: 366 KILEKVVEELDKVVGRERLVQESDIPKLKYLTACARESFRLHPFSSFNVPHISTSDIVVA 425

Query: 425 GYFIPKGSQVLLSRLGLGRNSRIWEDLMRFNPERHLKNGTIELGISEPDLRFVTFTRGRR 474
           GYFIPKGS+VLLSR GLGRN R+WED MRF+PERHLK+GT+ELGISEP+LRF+TFTRGRR
Sbjct: 426 GYFIPKGSEVLLSRSGLGRNPRVWEDPMRFDPERHLKDGTVELGISEPNLRFITFTRGRR 485

[Close]

BLAST of Lsi06G005660 vs. NCBI nr
Match: XP_022148957.1 (isoleucine N-monooxygenase 2-like [Momordica charantia])

HSP 1 Score: 647.5 bits (1669), Expect = 9.3e-182
Identity = 336/543 (61.88%), Postives = 402/543 (74.03%), Query Frame = 0


Query: 1   MKVLLFISESSYQNYAKESIAITFIAIIL--FISSIIYVIRFSQYYCKRPLSIRRLPPGP 60
           M+ LLFIS+ +YQN +  +I  TF   +   FIS+I     FSQ Y KR  S+ +LPPGP
Sbjct: 1   MEALLFISQLTYQN-SPTAITKTFTLFVFMAFISAI-----FSQCYRKRKHSVLQLPPGP 60

Query: 61  KPWPLLGYLPAMLSNKNLPTHHWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELALEFLK 120
            PWPL+G LPAML+ K+ P + WIH++MKQF+TEIACIRLG NT+++PVTSPELA+EFLK
Sbjct: 61  NPWPLVGCLPAMLATKS-PKYKWIHAVMKQFDTEIACIRLG-NTYVMPVTSPELAVEFLK 120

Query: 121 THDSVFRSR-PISMTAEIVSHGYLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQMLGQRT 180
            HDSVF SR  IS T  ++S G L+T  SP+G QWKKMRRIL S +   STLHQMLG RT
Sbjct: 121 RHDSVFASRSTISNTVNVLSRGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRT 180

Query: 181 KEADILLCYIFNL------SRNGEAINIKSIVRHYCSNIVRRMLFNKRYYGKGREDGGPT 240
            EAD LL YIF+L      +++G  +N++S+ +HYC NI+RRMLFNKRYYGKGREDGGP+
Sbjct: 181 NEADTLLRYIFSLTTTNTVNKDGAVVNVRSVTQHYCGNIIRRMLFNKRYYGKGREDGGPS 240

Query: 241 FEEEDHNQALLSILRHVNAFSISEFIPFLKLFDLDGHEKI-------------------- 300
           FEEE+HNQALL+ILRHVNAFSIS+F+P LK FDLDGHEKI                    
Sbjct: 241 FEEEEHNQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKRALKALWKYDEPIINERV 300

Query: 301 ----------------------------------------DLQLASIDNPSNAVEWAMAE 360
                                                   +LQ+A+IDNPSNAVEW MAE
Sbjct: 301 QQWKDGKREGAEDILDILISTKDGNGKSLLTIDEIKAQITELQIATIDNPSNAVEWTMAE 360

Query: 361 LLNQPKLLQKAVEELDEVVGRDRLVQEFDVPNLKYLTACIRESFRLHPFSPFNVPHVSNS 420
           LLNQPK+LQKA+EELD VVGR+RLVQE D+P LKYLTAC RESFRLHPFSPFN+PHVSN+
Sbjct: 361 LLNQPKILQKAIEELDRVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNIPHVSNA 420

Query: 421 DIVVAGYFIPKGSQVLLSRLGLGRNSRIWEDLMRFNPERHLKNGTIELGISEPDLRFVTF 474
           DIVVAGYFIPKGS+V+LSR GLGRN RIWED MRF+PERHLK+ T+ELG+SEP+LRF++F
Sbjct: 421 DIVVAGYFIPKGSEVILSRSGLGRNPRIWEDPMRFDPERHLKDETVELGLSEPNLRFISF 480

[Close]

BLAST of Lsi06G005660 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 449.1 bits (1154), Expect = 4.5e-126
Identity = 226/487 (46.41%), Postives = 311/487 (63.86%), Query Frame = 0


Query: 54  LPPGPKPWPLLGYLPAMLSNKNLPTHHWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELA 113
           LPPGPK WPL+G LP +L  +N P   WIHS+MK+ NT+IACIRL +NTH+IPVTSP +A
Sbjct: 34  LPPGPKSWPLIGNLPEIL-GRNKPVFRWIHSLMKELNTDIACIRL-ANTHVIPVTSPRIA 93

Query: 114 LEFLKTHDSVFRSRPISMTAEIVSHGYLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQML 173
            E LK  DSVF +RP++M  E  S GYL+  + P G+QWKKMRR++ SHV +  +   ML
Sbjct: 94  REILKKQDSVFATRPLTMGTEYCSRGYLTVAVEPQGEQWKKMRRVVASHVTSKKSFQMML 153

Query: 174 GQRTKEADILLCYIFNLS--RNGEA---INIKSIVRHYCSNIVRRMLFNKRYYGKGREDG 233
            +RT+EAD L+ YI N S    G A   I+++  VR Y  N+ R+M+F  R++GKG EDG
Sbjct: 154 QKRTEEADNLVRYINNRSVKNRGNAFVVIDLRLAVRQYSGNVARKMMFGIRHFGKGSEDG 213

Query: 234 -GPTFEEEDHNQALLSILRHVNAFSISEFIPFLKLFDLDGHEKI---------------- 293
            GP  EE +H ++L ++L H+ AF++S+++P+L+  DL+GHEK+                
Sbjct: 214 SGPGLEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVSNAMRNVSKYNDPFV 273

Query: 294 --------------------------------------------DLQLASIDNPSNAVEW 353
                                                       +L LA++DNPSNA EW
Sbjct: 274 DERLMQWRNGKMKEPQDFLDMFIIAKDTDGKPTLSDEEIKAQVTELMLATVDNPSNAAEW 333

Query: 354 AMAELLNQPKLLQKAVEELDEVVGRDRLVQEFDVPNLKYLTACIRESFRLHPFSPFNVPH 413
            MAE++N+P ++QKAVEE+D VVG+DRLV E D+PNL Y+ AC++E+FRLHP +PFN+PH
Sbjct: 334 GMAEMINEPSIMQKAVEEIDRVVGKDRLVIESDLPNLNYVKACVKEAFRLHPVAPFNLPH 393

Query: 414 VSNSDIVVAGYFIPKGSQVLLSRLGLGRNSRIWEDLMRFNPERHLKNGTIELGISEPDLR 473
           +S +D VV GYFIPKGS VL+SR+G+GRN  +W+   +F+PERHL   T  + ++E DL 
Sbjct: 394 MSTTDTVVDGYFIPKGSHVLISRMGIGRNPSVWDKPHKFDPERHLSTNTC-VDLNESDLN 453

[Close]

BLAST of Lsi06G005660 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 406.8 bits (1044), Expect = 2.6e-113
Identity = 207/489 (42.33%), Postives = 300/489 (61.35%), Query Frame = 0


Query: 47  RPLSIRRLPPGPKPWPLLGYLPAMLSNKNLPTHHWIHSIMKQFNTEIACIRLGSNTHIIP 106
           R   +  LPPGP  +P++G +PAML  KN P   W+HS+MK+ NTEIAC+RLG NTH+IP
Sbjct: 51  RNKKLHPLPPGPTGFPIVGMIPAML--KNRPVFRWLHSLMKELNTEIACVRLG-NTHVIP 110

Query: 107 VTSPELALEFLKTHDSVFRSRPISMTAEIVSHGYLSTVLSPMGDQWKKMRRILVSHVLNS 166
           VT P++A E  K  D++F SRP++   +I+S+GY + V++P G+Q+KKMR+++++ ++  
Sbjct: 111 VTCPKIAREIFKQQDALFASRPLTYAQKILSNGYKTCVITPFGEQFKKMRKVIMTEIVCP 170

Query: 167 STLHQMLGQRTKEADILLCYIFNLSRNGEAINIKSIVRHYCSNIVRRMLFNKRYYGKGRE 226
           +    +   R +E D L  +++N+ +N E ++++ + RHYC N ++R++F  R + +  E
Sbjct: 171 ARHRWLHDNRAEETDHLTAWLYNMVKNSEPVDLRFVTRHYCGNAIKRLMFGTRTFSEKTE 230

Query: 227 -DGGPTFEEEDHNQALLSILRHVNAFSISEFIPFLKLFDLDGHEKI-------------- 286
            DGGPT E+ +H  A+   L    AF IS+++P L   DL+GHEKI              
Sbjct: 231 ADGGPTLEDIEHMDAMFEGLGFTFAFCISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDP 290

Query: 287 ----------------------------------------------DLQLASIDNPSNAV 346
                                                         +L +A+ DNPSNAV
Sbjct: 291 IIDERIKMWREGKRTQIEDFLDIFISIKDEAGQPLLTADEIKPTIKELVMAAPDNPSNAV 350

Query: 347 EWAMAELLNQPKLLQKAVEELDEVVGRDRLVQEFDVPNLKYLTACIRESFRLHPFSPFNV 406
           EWA+AE++N+P++L KA+EE+D VVG++R VQE D+P L Y+ A IRE+FRLHP + FN+
Sbjct: 351 EWAIAEMINKPEILHKAMEEIDRVVGKERFVQESDIPKLNYVKAIIREAFRLHPVAAFNL 410

Query: 407 PHVSNSDIVVAGYFIPKGSQVLLSRLGLGRNSRIWEDLMRFNPERHLKNGTIELGISEPD 466
           PHV+ SD  VAGY IPKGSQVLLSR GLGRN ++W D + F PERHL N   E+ ++E D
Sbjct: 411 PHVALSDTTVAGYHIPKGSQVLLSRYGLGRNPKVWSDPLSFKPERHL-NECSEVTLTEND 470

Query: 467 LRFVTFTRGRRGCIGTSLGTNMTMMLFARLVQGFSWSLLSGITKIDF--SKTDELSLKPL 473
           LRF++F+ G+RGC   +LGT +T M+ ARL+QGF W L    T+++   S  D    KPL
Sbjct: 471 LRFISFSTGKRGCAAPALGTAITTMMLARLLQGFKWKLAGSETRVELMESSHDMFLSKPL 530

[Close]

BLAST of Lsi06G005660 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 401.4 bits (1030), Expect = 1.1e-111
Identity = 202/473 (42.71%), Postives = 297/473 (62.79%), Query Frame = 0


Query: 54  LPPGPKPWPLLGYLPAMLSNKNLPTHHWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELA 113
           LPPGP  WP++G +P ML  K+ P   W+HSIMKQ NTEIAC++LG NTH+I VT P++A
Sbjct: 56  LPPGPTGWPIIGMIPTML--KSRPVFRWLHSIMKQLNTEIACVKLG-NTHVITVTCPKIA 115

Query: 114 LEFLKTHDSVFRSRPISMTAEIVSHGYLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQML 173
            E LK  D++F SRP++   +I+S+GY + V++P GDQ+KKMR+++++ ++  +    + 
Sbjct: 116 REILKQQDALFASRPLTYAQKILSNGYKTCVITPFGDQFKKMRKVVMTELVCPARHRWLH 175

Query: 174 GQRTKEADILLCYIFNLSRNGEAINIKSIVRHYCSNIVRRMLFNKRYYGKG-REDGGPTF 233
            +R++E D L  +++N+ +N  +++ + + RHYC N +++++F  R + K    DGGPT 
Sbjct: 176 QKRSEENDHLTAWVYNMVKNSGSVDFRFMTRHYCGNAIKKLMFGTRTFSKNTAPDGGPTV 235

Query: 234 EEEDHNQALLSILRHVNAFSISEFIPFLKLFDLDGHEKI--------------------- 293
           E+ +H +A+   L    AF IS+++P L   DL+GHEKI                     
Sbjct: 236 EDVEHMEAMFEALGFTFAFCISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDERIK 295

Query: 294 ---------------------------------------DLQLASIDNPSNAVEWAMAEL 353
                                                  +L +A+ DNPSNAVEWAMAE+
Sbjct: 296 MWREGKRTQIEDFLDIFISIKDEQGNPLLTADEIKPTIKELVMAAPDNPSNAVEWAMAEM 355

Query: 354 LNQPKLLQKAVEELDEVVGRDRLVQEFDVPNLKYLTACIRESFRLHPFSPFNVPHVSNSD 413
           +N+P++L+KA+EE+D VVG++RLVQE D+P L Y+ A +RE+FRLHP + FN+PHV+ SD
Sbjct: 356 VNKPEILRKAMEEIDRVVGKERLVQESDIPKLNYVKAILREAFRLHPVAAFNLPHVALSD 415

Query: 414 IVVAGYFIPKGSQVLLSRLGLGRNSRIWEDLMRFNPERHLKNGTIELGISEPDLRFVTFT 464
             VAGY IPKGSQVLLSR GLGRN ++W D + F PERHL N   E+ ++E DLRF++F+
Sbjct: 416 TTVAGYHIPKGSQVLLSRYGLGRNPKVWADPLCFKPERHL-NECSEVTLTENDLRFISFS 475

[Close]

BLAST of Lsi06G005660 vs. TAIR 10
Match: AT5G35917.1 (cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene )

HSP 1 Score: 365.2 bits (936), Expect = 8.6e-101
Identity = 201/487 (41.27%), Postives = 280/487 (57.49%), Query Frame = 0


Query: 54  LPPGPKPWPLLGYLPAMLSNKNLPTHHWIHSIMKQFNTEIACIRLGSNTHIIPVTSPELA 113
           LPPGPK WPL+G LP +L  +N P   WIHS+M++ NT+IACIRL +NTH+IPVTSP +A
Sbjct: 42  LPPGPKSWPLVGNLPEIL-GRNKPVFRWIHSLMEELNTDIACIRL-ANTHVIPVTSPRIA 101

Query: 114 LEFLKTHDSVFRSRPISMTAEIVSHGYLSTVLSPMGDQWKKMRRILVSHVLNSSTLHQML 173
            E LK  DSVF  RP++M  E  S GYL+  +   G+QWKKMRR++ SHV +  +   ML
Sbjct: 102 REILKKQDSVFAIRPLTMGTEYCSRGYLTIAVQSQGEQWKKMRRVVASHVTSKKSFKLML 161

Query: 174 GQRTKEADILLCYIFNL------SRNGEA-INIKSIVRHYCSNIVRRMLFNKRYYGKGRE 233
            +RT+EAD L+ YI N       + NG A I+++ +VR Y  N+ R+M+F  R++GKG E
Sbjct: 162 EKRTEEADNLVRYINNRCVKNRGNGNGLAVIDLRFVVRQYSGNVARKMMFGIRHFGKGSE 221

Query: 234 D-GGPTFEEEDHNQALLSILRHVNAFSISEFIPFLKLFDLDGHEKI-------------- 293
           D  GP  EE +H ++L ++L H+ AF++S+++P+L+  DL+GHEK+              
Sbjct: 222 DESGPGSEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVADAMRNVSKYNDP 281

Query: 294 ----------------------------------------------DLQLASIDNPSNAV 353
                                                         +L LA++DNPSNA 
Sbjct: 282 FVDERLMQWRNGKMKEPQDFLDMFIMAKDTNGKPTLSEEEIKAQVTELMLATVDNPSNAA 341

Query: 354 EWAMAELLNQPKLLQKAVEELDEVVGRDRLVQEFDVPNLKYLTACIRESFRLHPFSPFNV 413
           EWAMAE++N+P ++QKAVEE+D    R+ + +               E+FRLHP +PFN+
Sbjct: 342 EWAMAEMINEPSIMQKAVEEIDRSNLRESVCE---------------ETFRLHPVAPFNL 401

Query: 414 PHVSNSDIVVAGYFIPKGSQVLLSRLGLGRNSRIWEDLMRFNPERHLKNGTIELGISEPD 471
           PH+S +D VV GYFIPKGS +L+SR+G+GRN  +W+  ++FNPERHL N           
Sbjct: 402 PHMSTADAVVDGYFIPKGSHMLISRMGIGRNPNVWDKPLKFNPERHLGN----------- 461

[Close]

BLAST of Lsi06G005660 vs. TAIR 10
Match: AT1G58260.1 (cytochrome p450 79c2 )

HSP 1 Score: 344.0 bits (881), Expect = 2.0e-94
Identity = 188/514 (36.58%), Postives = 299/514 (58.17%), Query Frame = 0


Query: 29  LFISSIIYVIRFSQYYCKRPLSIRRLPPGPKPWPLLGYLPAMLSNKNLPTHHWIHSIMKQ 88
           L + SI  V+  ++ + +      +LPPGP+ WP++G +  M+ N+  P H WIH +M++
Sbjct: 12  LVLISITLVLALARRFSRFMKPKGQLPPGPRGWPIVGNMLQMIINR--PAHLWIHRVMEE 71

Query: 89  FNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFRSRPISMTAEIVSHGYLSTVLSPM 148
             T+IAC R  +  H+I VTS ++A E L+  D V   R  S  + ++SHGY +   S  
Sbjct: 72  LQTDIACYRF-ARFHVITVTSSKIAREVLREKDEVLADRSESYASHLISHGYKNISFSSY 131

Query: 149 GDQWKKMRRILVSHVLNSSTLHQMLGQRTKEADILLCYIFNLSRNG---EAINIKSIVRH 208
           G+ WK +++++ + +++ +TL + LG R  EAD ++ Y++NL + G   + IN++  +  
Sbjct: 132 GENWKLVKKVMTTKLMSPTTLSKTLGYRNIEADNIVTYVYNLCQLGSVRKPINVRDTILT 191

Query: 209 YCSNIVRRMLFNKRYYGKGREDGGPTFEEEDHNQALLSILRHVNAFSISEFIPFLKLFDL 268
           YC  ++ RM+F +R++ +  E+GG   +E++H  A+   L    +F+++ +IPFL+ +++
Sbjct: 192 YCHAVMMRMMFGQRHFDEVVENGGLGPKEKEHMDAIYLALDCFFSFNLTNYIPFLRGWNV 251

Query: 269 DG--------------------HEKI---------------------------------- 328
           D                      E+I                                  
Sbjct: 252 DKAETEVREAVHIINICNDPIIQERIHLWRKKGGKQMEEDWLDILITLKDDQGMHLFTFD 311

Query: 329 -------DLQLASIDNPSNAVEWAMAELLNQPKLLQKAVEELDEVVGRDRLVQEFDVPNL 388
                  ++ LA+IDN  N VEW +AE+LN P++L+KA  ELD +VG+DRLVQE D+  L
Sbjct: 312 EIRAQCKEINLATIDNTMNNVEWTIAEMLNHPEILEKATNELDIIVGKDRLVQESDISQL 371

Query: 389 KYLTACIRESFRLHPFSPFNVPHVSNSDIVVAGYFIPKGSQVLLSRLGLGRNSRIWEDLM 448
            Y+ AC +ESFRLHP + F   HV+  D  +AGYF+PKGSQ+L+SRLGLGRN +IW++  
Sbjct: 372 NYIKACSKESFRLHPANVFMPHHVAREDTTLAGYFVPKGSQILVSRLGLGRNPKIWDEPN 431

Query: 449 RFNPERHLKNGTIE--LGIS--EPDLRFVTFTRGRRGCIGTSLGTNMTMMLFARLVQGFS 473
            F PER+L +G +E  LG++  EPD+RFVTF  GRR C GT +GT+MT+ML ARL+QGF 
Sbjct: 432 AFKPERYL-DGHVEKSLGVTLMEPDMRFVTFGTGRRSCPGTKIGTSMTIMLLARLIQGFE 491

The following BLAST results are available for this feature:

BLAST of Lsi06G005660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q605	2.0e-131	46.39	Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV...	[more]
Q6J541	1.7e-125	45.07	Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1	[more]
Q6J540	3.7e-125	45.07	Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1	[more]
Q9FLC8	6.4e-125	46.41	Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV...	[more]
Q43135	3.5e-123	45.13	Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3	[more]

BLAST of Lsi06G005660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VKT9	3.3e-209	72.87	Isoleucine N-monooxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3BPW4	3.0e-194	66.67	phenylalanine N-monooxygenase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1D6G9	4.5e-182	61.88	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498...	[more]
A0A1S3BNA6	5.9e-182	65.75	phenylalanine N-monooxygenase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1H9V7	1.6e-179	63.23	isoleucine N-monooxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461429 ...	[more]

BLAST of Lsi06G005660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011655052.1	1.1e-211	73.01	isoleucine N-monooxygenase 1 [Cucumis sativus] >KAE8648059.1 hypothetical protei...	[more]
KAA0067056.1	6.8e-209	72.87	isoleucine N-monooxygenase 1 [Cucumis melo var. makuwa] >TYK26293.1 isoleucine N...	[more]
XP_008450287.1	6.2e-194	66.67	PREDICTED: phenylalanine N-monooxygenase-like isoform X1 [Cucumis melo]	[more]
XP_038880438.1	3.2e-182	63.38	tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida]	[more]
XP_022148957.1	9.3e-182	61.88	isoleucine N-monooxygenase 2-like [Momordica charantia]	[more]

BLAST of Lsi06G005660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05260.1	4.5e-126	46.41	cytochrome p450 79a2	[more]
AT2G22330.1	2.6e-113	42.33	cytochrome P450, family 79, subfamily B, polypeptide 3	[more]
AT4G39950.1	1.1e-111	42.71	cytochrome P450, family 79, subfamily B, polypeptide 2	[more]
AT5G35917.1	8.6e-101	41.27	cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene	[more]
AT1G58260.1	2.0e-94	36.58	cytochrome p450 79c2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 276..293 score: 24.59 coord: 418..429 score: 26.28 coord: 409..418 score: 42.39 coord: 329..340 score: 39.75
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 271..463 e-value: 6.7E-47 score: 160.3 coord: 55..234 e-value: 1.1E-12 score: 47.4
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 418..441 score: 27.62 coord: 408..418 score: 43.8 coord: 369..393 score: 25.29 coord: 328..346 score: 37.67 coord: 112..133 score: 24.49
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 50..270 e-value: 8.2E-31 score: 109.0
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 271..472 e-value: 1.8E-53 score: 183.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 55..470
None	No IPR available	PANTHER	PTHR24298:SF675	PHENYLALANINE N-MONOOXYGENASE	coord: 29..271
None	No IPR available	PANTHER	PTHR24298:SF675	PHENYLALANINE N-MONOOXYGENASE	coord: 271..473
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 271..473 coord: 29..271

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G005660.1	Lsi06G005660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen