Lsi06G005650 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G005650
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionIsoleucine N-monooxygenase 1-like
Locationchr06: 7327359 .. 7329648 (-)
RNA-Seq ExpressionLsi06G005650
SyntenyLsi06G005650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCAACAATAATTAATTAAATGCTTTCAATCTCTTTTTTTATTCACAAATATAATAATGCCACTCATGTGTATATATTACATGCATATGTCTTAAAGGATATAACTACAAAGCATTCCACTACTACAAATAGAGAGAGTGAAAGAGGGAGGGATTGAGAGAAAGCCATGGAAGCTGCTTTCTTGTTCATTAGTCAATTGGCACACCAAAACTCTCCAATAGCCATATCCAAAACTTTCATTGTGTTTGTATTTATGACTTTTACTTCTATTATGTTCTCCCAATGTTATAACCGCAGAAGAAATCACTTAGTTCGAAAAACACCTCTTCCACCAGGCCCAAAGCCATTGCCTCTCGTTGGTTGCCTTCCAGCAATGTTGTCAAGGAACAACTCCTCAACACATCAATGGATTCATTCAGTAATGAAGCAATTCAACACTGAAATTGCTACCATACGCCTTGGAAATACTTACATAATCCCCGTCACTTCTCCAGAACTCGCTCTTCAATTTCTTAAGACATATGATTCTGTCTTTGCATCACGTTCCTCTATTTCCAATGTTGTTGAAATCTTAACACGTGGATATATCTCCACAATATTTTCACCATTGGGAGATCAATGGAAGAAGATGCGAAGAATTCTCGCATCAGAAATACTCAATCCATCAACTCTCCAACGAATGCTTGGCCAAAGAACTGGCGAAGCTGATACTCTTTTACGATATATTTTCACCCAAACCAATAATAATGAAGGAGGTGCAGTGATTAATGTTAGAAGCATAACACAACATTATTGCGGTAACATTATCCGAAGAATGTTATTCAATAGAAGATACTATGGAAAAGGGAGGGAAGATGGGGGGCCAACTTTTGAAGAAGAAGAACATAATCAAGCATTGTTGACCATTCTTAGGCATGTTAATGCATTTTCTATATCAGATTTCATGCCTTGTTTGAAGCCTTTTGATTTAGACGGACATCAGAAAACTATGAAAAATGCTTTGAATGTCCTTCGAAAGTATGACGACCTCATTATAGATGAGAGAGTGCAACAATGGAAGAAAGACGAGAAAATAGACAGGGTAGAGGACATTCTTGATATCCTCATTTCACTTAAAGATGGAAATGCAAATTCATTGTTGTCAATTGAAGAGATCAAGGCTCAAATCACAGTAAGTGTTCTACAACTTTATATATGATTAATAATCATGGATAAATTACTCGTACATACATATATGCACATGTGGTTAAATTATAATGGAACTTAGTCTCTCAACTTTTAAGTTTGCAATTATTTGGTTTGAACTTGAAAAGACTTCGATAAGATGTTTGACCTATTTGACAATTGTTTTTAATTTCATGGACCTTTCTACACACTAATTGAAAGTTTGATGACTTACTATTCAACTTTTTCCATCATTATAATAATAGGACTTACAAGTTGCAACGATAGATAATCCTTCAAACGCAGTGGAATGGGCAATGGCAGAATTAATCAACCAACCAAAAATTCTTGAAAAGGCCGTTGAAGAATTGGACGAAGTAGTTGGAAGAGAAAGGTTGGTTCAAGAATCTGATATCCCAAAACTCAAGTATCTCACAGCTTGTGCTAGAGAATCTTTTCGACTTCATCCATTTTCACCCTTCAATGTTCCTCACGTTGCCACCTCTGATATCATTGTGGCTGGCTACTTTATTCCCAAAGGAAGTGAAGTGTTGCTCAGTCGGTCAGGTCTTGGTCGAAATCCAAGGATTTGGGAGGATCCAATGAGATTTGGTCCAGAACGTCATCTAAAAGATGGGACAGCTGATTTGGGAATTTCAGAACCCAATTTGCGATTCATCACTTTCACTAGAGGAAGGAGAGGTTGTGCTGGTAGCTCACTTGGCACTAACATTACCATGATGTTGTTTGCTAGACTTCTACAAGGGTTTTCATGGAGTTTAATGCCAGGAATCACTAAGATAGACCTTTCTGATACTGATGATCTCTCTCTCTCGAAGCCATTGCATTTGCATGCAGAGCCACGTTTATCTCACAGTATGTACCCAAAGCTTTAAGCACACATAAACTACCTAAAAAAATGTCTACGAGATAAAAATAGGCAGTCCTTTTGCTTCCTACATATGCTTGGTGTTTAATTTCTATGGGCTGATCTTATGTATTGTAAAAATAATTTATGTTATATGTCATGTTTTTCTTAGTACCCTCACTCTTATTTGTGGAGGTAAATTAGAAAAATTTGTGTAAGCAAATATATATTAGAAATAAAGAACTTAATTTGGGTTT

mRNA sequence

TTCCAACAATAATTAATTAAATGCTTTCAATCTCTTTTTTTATTCACAAATATAATAATGCCACTCATGTGTATATATTACATGCATATGTCTTAAAGGATATAACTACAAAGCATTCCACTACTACAAATAGAGAGAGTGAAAGAGGGAGGGATTGAGAGAAAGCCATGGAAGCTGCTTTCTTGTTCATTAGTCAATTGGCACACCAAAACTCTCCAATAGCCATATCCAAAACTTTCATTGTGTTTGTATTTATGACTTTTACTTCTATTATGTTCTCCCAATGTTATAACCGCAGAAGAAATCACTTAGTTCGAAAAACACCTCTTCCACCAGGCCCAAAGCCATTGCCTCTCGTTGGTTGCCTTCCAGCAATGTTGTCAAGGAACAACTCCTCAACACATCAATGGATTCATTCAGTAATGAAGCAATTCAACACTGAAATTGCTACCATACGCCTTGGAAATACTTACATAATCCCCGTCACTTCTCCAGAACTCGCTCTTCAATTTCTTAAGACATATGATTCTGTCTTTGCATCACGTTCCTCTATTTCCAATGTTGTTGAAATCTTAACACGTGGATATATCTCCACAATATTTTCACCATTGGGAGATCAATGGAAGAAGATGCGAAGAATTCTCGCATCAGAAATACTCAATCCATCAACTCTCCAACGAATGCTTGGCCAAAGAACTGGCGAAGCTGATACTCTTTTACGATATATTTTCACCCAAACCAATAATAATGAAGGAGGTGCAGTGATTAATGTTAGAAGCATAACACAACATTATTGCGGTAACATTATCCGAAGAATGTTATTCAATAGAAGATACTATGGAAAAGGGAGGGAAGATGGGGGGCCAACTTTTGAAGAAGAAGAACATAATCAAGCATTGTTGACCATTCTTAGGCATGTTAATGCATTTTCTATATCAGATTTCATGCCTTGTTTGAAGCCTTTTGATTTAGACGGACATCAGAAAACTATGAAAAATGCTTTGAATGTCCTTCGAAAGTATGACGACCTCATTATAGATGAGAGAGTGCAACAATGGAAGAAAGACGAGAAAATAGACAGGGTAGAGGACATTCTTGATATCCTCATTTCACTTAAAGATGGAAATGCAAATTCATTGTTGTCAATTGAAGAGATCAAGGCTCAAATCACAGACTTACAAGTTGCAACGATAGATAATCCTTCAAACGCAGTGGAATGGGCAATGGCAGAATTAATCAACCAACCAAAAATTCTTGAAAAGGCCGTTGAAGAATTGGACGAAGTAGTTGGAAGAGAAAGGTTGGTTCAAGAATCTGATATCCCAAAACTCAAGTATCTCACAGCTTGTGCTAGAGAATCTTTTCGACTTCATCCATTTTCACCCTTCAATGTTCCTCACGTTGCCACCTCTGATATCATTGTGGCTGGCTACTTTATTCCCAAAGGAAGTGAAGTGTTGCTCAGTCGGTCAGGTCTTGGTCGAAATCCAAGGATTTGGGAGGATCCAATGAGATTTGGTCCAGAACGTCATCTAAAAGATGGGACAGCTGATTTGGGAATTTCAGAACCCAATTTGCGATTCATCACTTTCACTAGAGGAAGGAGAGGTTGTGCTGGTAGCTCACTTGGCACTAACATTACCATGATGTTGTTTGCTAGACTTCTACAAGGGTTTTCATGGAGTTTAATGCCAGGAATCACTAAGATAGACCTTTCTGATACTGATGATCTCTCTCTCTCGAAGCCATTGCATTTGCATGCAGAGCCACGTTTATCTCACAGTATGTACCCAAAGCTTTAAGCACACATAAACTACCTAAAAAAATGTCTACGAGATAAAAATAGGCAGTCCTTTTGCTTCCTACATATGCTTGGTGTTTAATTTCTATGGGCTGATCTTATGTATTGTAAAAATAATTTATGTTATATGTCATGTTTTTCTTAGTACCCTCACTCTTATTTGTGGAGGTAAATTAGAAAAATTTGTGTAAGCAAATATATATTAGAAATAAAGAACTTAATTTGGGTTT

Coding sequence (CDS)

ATGGAAGCTGCTTTCTTGTTCATTAGTCAATTGGCACACCAAAACTCTCCAATAGCCATATCCAAAACTTTCATTGTGTTTGTATTTATGACTTTTACTTCTATTATGTTCTCCCAATGTTATAACCGCAGAAGAAATCACTTAGTTCGAAAAACACCTCTTCCACCAGGCCCAAAGCCATTGCCTCTCGTTGGTTGCCTTCCAGCAATGTTGTCAAGGAACAACTCCTCAACACATCAATGGATTCATTCAGTAATGAAGCAATTCAACACTGAAATTGCTACCATACGCCTTGGAAATACTTACATAATCCCCGTCACTTCTCCAGAACTCGCTCTTCAATTTCTTAAGACATATGATTCTGTCTTTGCATCACGTTCCTCTATTTCCAATGTTGTTGAAATCTTAACACGTGGATATATCTCCACAATATTTTCACCATTGGGAGATCAATGGAAGAAGATGCGAAGAATTCTCGCATCAGAAATACTCAATCCATCAACTCTCCAACGAATGCTTGGCCAAAGAACTGGCGAAGCTGATACTCTTTTACGATATATTTTCACCCAAACCAATAATAATGAAGGAGGTGCAGTGATTAATGTTAGAAGCATAACACAACATTATTGCGGTAACATTATCCGAAGAATGTTATTCAATAGAAGATACTATGGAAAAGGGAGGGAAGATGGGGGGCCAACTTTTGAAGAAGAAGAACATAATCAAGCATTGTTGACCATTCTTAGGCATGTTAATGCATTTTCTATATCAGATTTCATGCCTTGTTTGAAGCCTTTTGATTTAGACGGACATCAGAAAACTATGAAAAATGCTTTGAATGTCCTTCGAAAGTATGACGACCTCATTATAGATGAGAGAGTGCAACAATGGAAGAAAGACGAGAAAATAGACAGGGTAGAGGACATTCTTGATATCCTCATTTCACTTAAAGATGGAAATGCAAATTCATTGTTGTCAATTGAAGAGATCAAGGCTCAAATCACAGACTTACAAGTTGCAACGATAGATAATCCTTCAAACGCAGTGGAATGGGCAATGGCAGAATTAATCAACCAACCAAAAATTCTTGAAAAGGCCGTTGAAGAATTGGACGAAGTAGTTGGAAGAGAAAGGTTGGTTCAAGAATCTGATATCCCAAAACTCAAGTATCTCACAGCTTGTGCTAGAGAATCTTTTCGACTTCATCCATTTTCACCCTTCAATGTTCCTCACGTTGCCACCTCTGATATCATTGTGGCTGGCTACTTTATTCCCAAAGGAAGTGAAGTGTTGCTCAGTCGGTCAGGTCTTGGTCGAAATCCAAGGATTTGGGAGGATCCAATGAGATTTGGTCCAGAACGTCATCTAAAAGATGGGACAGCTGATTTGGGAATTTCAGAACCCAATTTGCGATTCATCACTTTCACTAGAGGAAGGAGAGGTTGTGCTGGTAGCTCACTTGGCACTAACATTACCATGATGTTGTTTGCTAGACTTCTACAAGGGTTTTCATGGAGTTTAATGCCAGGAATCACTAAGATAGACCTTTCTGATACTGATGATCTCTCTCTCTCGAAGCCATTGCATTTGCATGCAGAGCCACGTTTATCTCACAGTATGTACCCAAAGCTTTAA

Protein sequence

MEAAFLFISQLAHQNSPIAISKTFIVFVFMTFTSIMFSQCYNRRRNHLVRKTPLPPGPKPLPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFLKTYDSVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQRTGEADTLLRYIFTQTNNNEGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEEEHNQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQWKKDEKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAELINQPKILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSDIIVAGYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGTADLGISEPNLRFITFTRGRRGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDTDDLSLSKPLHLHAEPRLSHSMYPKL
Homology
BLAST of Lsi06G005650 vs. ExPASy Swiss-Prot
Match: A0A068Q605 (Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 1.3e-160
Identity = 280/545 (51.38%), Postives = 387/545 (71.01%), Query Frame = 0

Query: 1   MEAAFLFISQLAHQNSPIAISKTFIVFVFMTFTSIMFSQCYNRRRNHLVRKTPLPPGPKP 60
           M   +LF+   +    PI++S  FI+F+F+                + V   PLPPGP P
Sbjct: 1   MSLPYLFLD--SEVTPPISLSLAFIIFMFLV------KFILKTHNKNSVPVVPLPPGPSP 60

Query: 61  LPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFLKTYD 120
            P+VG LP M    N   H+WIHS+MK+ NT+IA IRLGN ++IPVTSPE+A +FLK  D
Sbjct: 61  WPIVGSLPEMW--RNRPAHRWIHSLMKKLNTDIACIRLGNVHVIPVTSPEIAREFLKKND 120

Query: 121 SVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQRTGEA 180
           +VFASR  ++   + L+ GY++T+  P GDQW+KMRR+L +E  NPS +  +LG+R  EA
Sbjct: 121 AVFASR-PVTMATKTLSSGYLTTVVGPWGDQWRKMRRVLVAEAFNPSRVHWLLGKRNEEA 180

Query: 181 DTLLRYIFTQTNNNEGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEEEH 240
           D L+++++ Q + N+ GAV+NVR   Q Y G+I+R+M+FNR Y+GKGREDGGP  EEEEH
Sbjct: 181 DNLVKFLYNQCSANQNGAVVNVRIAAQFYSGSIMRKMIFNRTYFGKGREDGGPGVEEEEH 240

Query: 241 NQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQWKKD 300
             ALLT+L +  AF +SD++P L+ FD+DGH+K ++ A+N+++K+ + I++ER+Q+W +D
Sbjct: 241 VSALLTLLTYAYAFCVSDYLPWLRVFDIDGHEKKVRKAMNIVKKHQEPIVNERLQEW-RD 300

Query: 301 EKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAELINQP 360
            K +  +D+LD+ ISLKD N   LLS EEIKAQ T+LQ++T+D+P N  EWA+ E++NQP
Sbjct: 301 GKRNEPDDLLDVFISLKDANGQPLLSDEEIKAQTTELQLSTVDSPFNIAEWALTEMLNQP 360

Query: 361 KILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSDIIVA 420
           ++L+KA EELD VVG++ LVQES +P L Y+ ACA+E  RLHP  PFN+PHV+ +D  VA
Sbjct: 361 EMLKKAEEELDRVVGKKTLVQESHVPHLPYIRACAKEVMRLHPVGPFNLPHVSIADAEVA 420

Query: 421 GYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGT-ADLGISEPNLRFITFTRGR 480
           GYFIPKGS V+LSR  LGRNPR+WE+P+RF PERHL       + + E +LRF++F+ GR
Sbjct: 421 GYFIPKGSNVILSRLELGRNPRVWEEPLRFNPERHLNIAVDQQVDLEENDLRFVSFSTGR 480

Query: 481 RGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDTDDLSLSK--PLHLHAEPRLS 540
           RGC G  LG+ I +ML ARLLQGFSWSL P + KID ++ D + L K  PL   A+PRL 
Sbjct: 481 RGCMGVGLGSTIVVMLLARLLQGFSWSLPPDVDKIDFTE-DQIYLKKASPLLAQAKPRLP 532

Query: 541 HSMYP 543
            S+YP
Sbjct: 541 ASVYP 532

BLAST of Lsi06G005650 vs. ExPASy Swiss-Prot
Match: Q6J541 (Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 6.5e-155
Identity = 276/525 (52.57%), Postives = 374/525 (71.24%), Query Frame = 0

Query: 23  TFIVFVFMTFTSIMFSQCYNRRRNHLVRKT---PLPPGPKPLPLVGCLPAMLSRNNSSTH 82
           TF++ V  +F        +   + HLV K+    LPPGPKP P+VG LP ML+  N    
Sbjct: 17  TFLLVVIFSF------MIFKVTKTHLVNKSKKYKLPPGPKPWPIVGNLPEMLA--NRPAT 76

Query: 83  QWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFLKTYDSVFASRSSISNVVEILTRG 142
            WIH +MK+ NTEIA IRL NT +IPVT P +A +FLK +D+ FASR  I +  +I + G
Sbjct: 77  IWIHKLMKEMNTEIACIRLANTIVIPVTCPTIACEFLKKHDASFASRPKIMS-TDIASDG 136

Query: 143 YISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQRTGEADTLLRYIFTQ--TNNNEGG 202
           +I+T+  P G+QWKKM+R+L + +L+P   Q +LG+R  EAD L+ YI+ +   + N+G 
Sbjct: 137 FITTVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKDVNDGP 196

Query: 203 AVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEEEHNQALLTILRHVNAFSIS 262
            ++N+R   QHY GN+ R+++FN RY+GK  EDGGP FEE EH  A  TIL++V AFSIS
Sbjct: 197 GLVNIRIAAQHYGGNVFRKLIFNSRYFGKVMEDGGPGFEEVEHINATFTILKYVYAFSIS 256

Query: 263 DFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQWKKDEKIDRVEDILDILISLK 322
           DF+P L+  DLDGH+  +  A+ ++RKY D IID+R++QW    K    ED+LD+LI LK
Sbjct: 257 DFVPFLRRLDLDGHRSKIMKAMRIMRKYHDPIIDDRIKQWNDGLKTVE-EDLLDVLIKLK 316

Query: 323 DGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAELINQPKILEKAVEELDEVVGRE 382
           D N   LL+++E+KAQI +L +  +DNPSNA EWA+AE+INQP++L++A EELD VVG+E
Sbjct: 317 DANNKPLLTLKELKAQIIELAIEMVDNPSNAFEWALAEMINQPELLKRATEELDNVVGKE 376

Query: 383 RLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSDIIVAGYFIPKGSEVLLSRSGL 442
           RLVQESDIPKL+++ ACARE+ RLHP   FNVPH+  +D +V  Y  PKG++VLLSR  L
Sbjct: 377 RLVQESDIPKLQFVKACAREALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQVLLSRVAL 436

Query: 443 GRNPRIWEDPMRFGPERHLKDGTADLGISEPNLRFITFTRGRRGCAGSSLGTNITMMLFA 502
           GRNP+ W DP++F PERHLK+G  D+ ++EP+LRFI+FT GRR C G +LGT +T+MLFA
Sbjct: 437 GRNPKFWTDPLKFNPERHLKEG-IDVVLTEPDLRFISFTTGRRSCPGVALGTTMTVMLFA 496

Query: 503 RLLQGFSWSLMPGITKIDL-SDTDDLSLSKPLHLHAEPRLSHSMY 542
           R+L GFSWS  P ++ IDL    DDL L+KPL L A+PRL+  +Y
Sbjct: 497 RMLHGFSWSPPPDVSSIDLVPSKDDLFLAKPLLLVAKPRLAAELY 530

BLAST of Lsi06G005650 vs. ExPASy Swiss-Prot
Match: Q6J540 (Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 2.7e-153
Identity = 269/527 (51.04%), Postives = 378/527 (71.73%), Query Frame = 0

Query: 18  IAISKTFIVFVFMTFTSIMFSQCYNRRRNHLVRKTPLPPGPKPLPLVGCLPAMLSRNNSS 77
           +  S TF++ V   F     ++ ++  ++   +K  LPPGPKP P+VG LP ML+  N  
Sbjct: 12  LEFSWTFLLVVIFFFIIFKVTKSHSVNKS---KKYKLPPGPKPWPIVGNLPEMLA--NRP 71

Query: 78  THQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFLKTYDSVFASRSSISNVVEILT 137
              WIH +MK+ NTEIA IRL NT +IPVT P +A +FLK +D+ FASR  I +  +I +
Sbjct: 72  ATIWIHKLMKEMNTEIACIRLANTIVIPVTCPTIACEFLKKHDASFASRPKIMS-TDIAS 131

Query: 138 RGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQRTGEADTLLRYIFTQ--TNNNE 197
            G+++T+  P G+QWKKM+R+L + +L+P   Q +LG+R  EAD L+ YI+ +   + N+
Sbjct: 132 DGFLTTVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKDVND 191

Query: 198 GGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEEEHNQALLTILRHVNAFS 257
           G  ++N+R   QHY GN+ R+++FN RY+GK  EDGGP FEE EH  A  TIL++V AFS
Sbjct: 192 GPGLVNIRIAAQHYGGNVFRKLIFNTRYFGKVMEDGGPGFEEVEHINATFTILKYVYAFS 251

Query: 258 ISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQWKKDEKIDRVEDILDILIS 317
           ISDF+P L+  DLDGH+  +  A+ +++KY D II +R++QW    K    ED+LD+LI 
Sbjct: 252 ISDFIPFLRRLDLDGHRSKIMKAMGIMKKYHDPIIHDRIKQWNDGLKTVE-EDLLDVLIK 311

Query: 318 LKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAELINQPKILEKAVEELDEVVG 377
           LKD +   LL+++EIKAQIT+L +  +DNPSNA EWA+AE++NQP++L++A EELD VVG
Sbjct: 312 LKDASNKPLLTLKEIKAQITELAIEMVDNPSNAFEWALAEMLNQPELLKRATEELDNVVG 371

Query: 378 RERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSDIIVAGYFIPKGSEVLLSRS 437
           +ERLVQESDIPKL+++ ACARE+ RLHP   FNVPH+  +D +V  Y  PKG++VLLSR 
Sbjct: 372 KERLVQESDIPKLQFVKACAREALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQVLLSRV 431

Query: 438 GLGRNPRIWEDPMRFGPERHLKDGTADLGISEPNLRFITFTRGRRGCAGSSLGTNITMML 497
            LGRNP+ W DP++F PERHLK+G  D+ ++EP+LRFI+FT GRR C G +LGT +T+ML
Sbjct: 432 ALGRNPKFWTDPLKFNPERHLKEG-IDVVLTEPDLRFISFTTGRRSCPGVTLGTTMTIML 491

Query: 498 FARLLQGFSWSLMPGITKIDLS-DTDDLSLSKPLHLHAEPRLSHSMY 542
           FAR+L GFSWS  P ++ IDL+  +DDL ++KPL + A+PRL+  +Y
Sbjct: 492 FARMLHGFSWSAPPNVSSIDLTQSSDDLFMAKPLCVVAKPRLAAELY 530

BLAST of Lsi06G005650 vs. ExPASy Swiss-Prot
Match: Q43135 (Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3)

HSP 1 Score: 527.3 bits (1357), Expect = 2.0e-148
Identity = 255/498 (51.20%), Postives = 353/498 (70.88%), Query Frame = 0

Query: 49  VRKTPLPPGPKPLPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTS 108
           V   PLPPGP P P+VG LP ML   N    +WIH +M++  T+IA ++LG  +++ +T 
Sbjct: 63  VGNPPLPPGPVPWPVVGNLPEMLL--NKPAFRWIHQMMREMGTDIACVKLGGVHVVSITC 122

Query: 109 PELALQFLKTYDSVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPST 168
           PE+A + L+  D+ F SR  ++   E  + GY + + SP GDQWKKMRR+L SEI+ PS 
Sbjct: 123 PEIAREVLRKQDANFISR-PLTFASETFSGGYRNAVLSPYGDQWKKMRRVLTSEIICPSR 182

Query: 169 LQRMLGQRTGEADTLLRYIFT-QTNNNEGGAVINVRSITQHYCGNIIRRMLFNRRYYGKG 228
              +  +RT EAD L RY++   T    G   ++VR + +HYCGN+IRR++FNRRY+G+ 
Sbjct: 183 HAWLHDKRTDEADNLTRYVYNLATKAATGDVAVDVRHVARHYCGNVIRRLMFNRRYFGEP 242

Query: 229 REDGGPTFEEEEHNQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYDD 288
           + DGGP   E  H  A+ T L  + AF +SD++P L+  DLDGH+K +K A   + +  D
Sbjct: 243 QADGGPGPMEVLHMDAVFTSLGLLYAFCVSDYLPWLRGLDLDGHEKIVKEANVAVNRLHD 302

Query: 289 LIIDERVQQWKKDEKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPSN 348
            +ID+R +QWK  E+   +ED LD+LI+LKD   N LL+IEE+KAQ  D+  A +DNPSN
Sbjct: 303 TVIDDRWRQWKSGER-QEMEDFLDVLITLKDAQGNPLLTIEEVKAQSQDITFAAVDNPSN 362

Query: 349 AVEWAMAELINQPKILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSPF 408
           AVEWA+AE++N P+++ KA+EELD VVGRERLVQESDIPKL Y+ AC RE+FRLHP +PF
Sbjct: 363 AVEWALAEMVNNPEVMAKAMEELDRVVGRERLVQESDIPKLNYVKACIREAFRLHPVAPF 422

Query: 409 NVPHVATSDIIVAGYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGTADLGISE 468
           NVPHVA +D  +AGY +PKGS V+LSR+GLGRNPR+W++P+RF P+RHL    +D+ ++E
Sbjct: 423 NVPHVALADTTIAGYRVPKGSHVILSRTGLGRNPRVWDEPLRFYPDRHLATAASDVALTE 482

Query: 469 PNLRFITFTRGRRGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDT-DDLSLSK 528
            +LRFI+F+ GRRGC  +SLGT +++MLF RLLQGF+WS   G+  +DLS++  D  ++ 
Sbjct: 483 NDLRFISFSTGRRGCIAASLGTAMSVMLFGRLLQGFTWSKPAGVEAVDLSESKSDTFMAT 542

Query: 529 PLHLHAEPRLSHSMYPKL 545
           PL LHAEPRL   +YP +
Sbjct: 543 PLVLHAEPRLPAHLYPSI 556

BLAST of Lsi06G005650 vs. ExPASy Swiss-Prot
Match: A0A068Q7V0 (Phenylalanine N-monooxygenase CYP79D16 OS=Prunus mume OX=102107 GN=CYP79D16 PE=1 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 7.2e-146
Identity = 251/505 (49.70%), Postives = 365/505 (72.28%), Query Frame = 0

Query: 42  NRRRNHLVRK-TPLPPGPK---PLPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIR 101
           N + N  +++  PLPP PK   P P+VG LP ML   N  T +WIH +M++ NT+IA IR
Sbjct: 31  NDKNNKAIKQHYPLPPTPKGLRPWPIVGNLPEMLM--NKPTFRWIHKLMEESNTQIACIR 90

Query: 102 LGNTYIIPVTSPELALQFLKTYDSVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRR 161
           L N ++ PV+ P L+ + LK  D+ FA+R  +S    ++T+GYI+T+  P G+QWKKMR+
Sbjct: 91  LANVHVTPVSCPILSREILKKQDATFATR-PLSISTFLITKGYITTVMVPFGEQWKKMRK 150

Query: 162 ILASEILNPSTLQRMLGQRTGEADTLLRYIFTQTNNNEGGAVINVRSITQHYCGNIIRRM 221
           ++ SE+L+P   + +  +R  EAD L+RY+F Q NN+EG  ++++R  TQHYC N+I+RM
Sbjct: 151 VITSELLSPMRHKWLTDKRIEEADHLVRYVFNQCNNDEGSGIVDLRLATQHYCANVIKRM 210

Query: 222 LFNRRYYGKGREDGGPTFEEEEHNQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKN 281
           +FN+RY+ +  +DGGP+ EE+ +  A+  +LR++ AFS SD++ CL+  DLDGH+K +K+
Sbjct: 211 IFNQRYFTEEMKDGGPSVEEQNYVNAVFDMLRYIYAFSASDYISCLRGLDLDGHEKIIKD 270

Query: 282 ALNVLRKYDDLIIDERVQQWKKDEKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDL 341
            + + RK  D +I+ER+++ +K        D+LDILISLKD +   LLS +EIK Q+ ++
Sbjct: 271 CIKLTRKRQDPVIEERIREHQKLGGNKVPVDLLDILISLKDASGQPLLSTDEIKGQVNEM 330

Query: 342 QVATIDNPSNAVEWAMAELINQPKILEKAVEELDEVVGRERLVQESDIPKLKYLTACARE 401
            +A +DNPSNA EWA+AE+INQP + EKA +ELD VVG+ER VQESD+ +L ++ ACARE
Sbjct: 331 IMAAVDNPSNAAEWAIAEMINQPHLFEKARQELDAVVGKERQVQESDLSQLNFVKACARE 390

Query: 402 SFRLHPFSPFNVPHVATSDIIVAGYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLK 461
           +FRLHP +PFNVPHV+ +D  V  YFIPKGS V+LSR GLGRNP+IW++P+++ PERHLK
Sbjct: 391 AFRLHPVAPFNVPHVSMADTTVGDYFIPKGSHVMLSRIGLGRNPKIWDEPLKYKPERHLK 450

Query: 462 DGTADLGISEPNLRFITFTRGRRGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLS 521
           D  + + ++E  LRFI+F+ G RGC  S+LGT++T+MLFARLL GF+W   P  ++IDL+
Sbjct: 451 DDGSGVVLTESELRFISFSTGMRGCVASTLGTSMTVMLFARLLHGFTWEAPPNESRIDLT 510

Query: 522 DTD-DLSLSKPLHLHAEPRLSHSMY 542
           + D +L L+KPL   A+PRL   +Y
Sbjct: 511 EADGELLLAKPLFALAKPRLPAHVY 532

BLAST of Lsi06G005650 vs. ExPASy TrEMBL
Match: A0A5D3DVF9 (Isoleucine N-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold225G00730 PE=3 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 1.7e-251
Identity = 432/545 (79.27%), Postives = 474/545 (86.97%), Query Frame = 0

Query: 1   MEAAFLFISQLAHQNSPIA----ISKTFIVFVFMTFTSIMFSQCYNRRRNHLVRKTPLPP 60
           ME A +F  QL +QNSP+A    +  +F++F+FM F SI+F QCYNR   H   + PLPP
Sbjct: 1   MEVASIFTGQLIYQNSPVAMISNLQPSFVMFLFMAFLSILFFQCYNRTEKHSNLQPPLPP 60

Query: 61  GPKPLPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFL 120
           GPKP PL+GCLPAMLS NNSSTH+WIHS+MKQFN EIA+IRL NTY+IPV SPELAL+FL
Sbjct: 61  GPKPWPLLGCLPAMLSSNNSSTHEWIHSIMKQFNAEIASIRLRNTYVIPVASPELALEFL 120

Query: 121 KTYDSVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQR 180
           KTYDSVF SRSS SN V++LT G +STI SP G QW+KM+RIL SEILNPSTL RMLGQR
Sbjct: 121 KTYDSVFGSRSSFSNDVDMLTGGCVSTILSPTGPQWRKMKRILTSEILNPSTLHRMLGQR 180

Query: 181 TGEADTLLRYIFTQTNNNEGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFE 240
           T EAD LL YIF QT  N GGAVINVRSITQHYCGNIIRR++FNRRYYGKGREDGGPTFE
Sbjct: 181 TAEADALLHYIFNQTCKNGGGAVINVRSITQHYCGNIIRRIVFNRRYYGKGREDGGPTFE 240

Query: 241 EEEHNQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQ 300
           EEEHNQALLTI+RHVN+FSISDFMPCLKP DLDGHQK MKNALNVLRKYD+ II ERVQQ
Sbjct: 241 EEEHNQALLTIVRHVNSFSISDFMPCLKPLDLDGHQKNMKNALNVLRKYDEHIIKERVQQ 300

Query: 301 WKKDEKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAEL 360
           WK DEKI  VEDILDILISL D N NSLLSIEEIK QI D+Q+ATIDNPSNAVEWAMAEL
Sbjct: 301 WKNDEKIKGVEDILDILISLTDDNGNSLLSIEEIKNQIMDIQLATIDNPSNAVEWAMAEL 360

Query: 361 INQPKILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSD 420
           +NQPK+L+KA+EELD+VVGRERLVQESDI  LKYLTACARESFRLHPFSPFNV HV+ SD
Sbjct: 361 LNQPKVLKKAIEELDKVVGRERLVQESDISNLKYLTACARESFRLHPFSPFNVAHVSNSD 420

Query: 421 IIVAGYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGTADLGISEPNLRFITFT 480
           I+VAGYFIPKGSEVLLSR GLGRNPRIWEDPM+F PERHLKDGT +LGISEPNLRFITFT
Sbjct: 421 IVVAGYFIPKGSEVLLSRLGLGRNPRIWEDPMKFNPERHLKDGTIELGISEPNLRFITFT 480

Query: 481 RGRRGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDTDDLSLSKPLHLHAEPRL 540
           RGRRGC GSSLGTNITMMLFARLLQGFSW+ +P  TK DL  T++LSL KPLHLHA+PRL
Sbjct: 481 RGRRGCPGSSLGTNITMMLFARLLQGFSWTSLPKFTKTDLPQTNELSLLKPLHLHAKPRL 540

Query: 541 SHSMY 542
           SH MY
Sbjct: 541 SHDMY 545

BLAST of Lsi06G005650 vs. ExPASy TrEMBL
Match: A0A1S3BQ37 (isoleucine N-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103491994 PE=3 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 6.5e-251
Identity = 436/546 (79.85%), Postives = 486/546 (89.01%), Query Frame = 0

Query: 1   MEAAF-LFISQLAHQNSPIAISKTFIVFVFMTFTSIMFSQCYNRRRNHLVRKTPLPPGPK 60
           MEAAF +FI+QL HQNSPIAISK FIVF+FM F S+MFS+CYNRR+ + VRK  LPPGPK
Sbjct: 1   MEAAFSIFITQLPHQNSPIAISKAFIVFLFMAFASVMFSRCYNRRKKYSVRKALLPPGPK 60

Query: 61  PLPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFLKTY 120
           P P+VGCLP ML+ N S T+QWIHSVMKQFNTEIA IRLGNTY+IPVTSPELAL+FL+T+
Sbjct: 61  PWPIVGCLPTMLT-NKSPTYQWIHSVMKQFNTEIACIRLGNTYVIPVTSPELALEFLRTH 120

Query: 121 DSVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQRTGE 180
           DSVF+SRSSISN V+IL+RGY++T FS +GDQWKKMRRILASEIL+P+ L RML QR  E
Sbjct: 121 DSVFSSRSSISNTVDILSRGYLTTAFSSMGDQWKKMRRILASEILSPTMLHRMLEQRIAE 180

Query: 181 ADTLLRYIFTQTNNNEGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEEE 240
           ADTLL YIF+ T+ N GGAVINVRSITQHYCGNIIR MLFNRRYYGKGREDGGPT EEEE
Sbjct: 181 ADTLLHYIFSITSENGGGAVINVRSITQHYCGNIIRTMLFNRRYYGKGREDGGPTSEEEE 240

Query: 241 HNQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQWKK 300
           HNQALLTILRH+NAFSISDFMPCLKPFDLDGH+K MKNALN +RKYD+ IIDERVQQWKK
Sbjct: 241 HNQALLTILRHINAFSISDFMPCLKPFDLDGHEKIMKNALNAVRKYDEPIIDERVQQWKK 300

Query: 301 DEKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAELINQ 360
           D+KI+ V+DILDILIS KD N NSLLSI+EIKAQIT+LQ+ATIDNPSNAVEWAMAELINQ
Sbjct: 301 DKKIE-VKDILDILISRKDDNGNSLLSIDEIKAQITELQIATIDNPSNAVEWAMAELINQ 360

Query: 361 PKILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSDIIV 420
           P++L+K V+ELD VVG+ERLVQE DIPKLKYLTAC RESFRLHPFSPFNVPHV T DI+V
Sbjct: 361 PEVLKKVVDELDNVVGKERLVQEYDIPKLKYLTACLRESFRLHPFSPFNVPHVPTFDIVV 420

Query: 421 AGYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGTADLGISEPNLRFITFTRGR 480
            GY IPKGSEVLLSR GLGRNPRIW+DP++F PERHLKD T +LG+SEPNLRFITF+RGR
Sbjct: 421 NGYLIPKGSEVLLSRLGLGRNPRIWKDPIKFNPERHLKDNTVELGLSEPNLRFITFSRGR 480

Query: 481 RGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDTD-DLSLSKPLHLHAEPRLSH 540
           RGC GSSLGTNIT+MLFARLLQGFSWSL+PGIT+ID S  D +  L  PL LHAEPRLS 
Sbjct: 481 RGCVGSSLGTNITVMLFARLLQGFSWSLLPGITQIDFSKVDREGLLLNPLQLHAEPRLSR 540

Query: 541 SMYPKL 545
           SMYP L
Sbjct: 541 SMYPML 544

BLAST of Lsi06G005650 vs. ExPASy TrEMBL
Match: A0A1S3BNG9 (isoleucine N-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103491996 PE=3 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 7.0e-245
Identity = 426/542 (78.60%), Postives = 471/542 (86.90%), Query Frame = 0

Query: 1   MEAAFLFISQLAHQNSPIAI-SKTFIVFVFMTFTSIMFSQCYNRRRNHLVRKTPLPPGPK 60
           ME A +FI+QL +QNSPI I SK  I F+ M FTSI+F QCYNR R H   + PLPPGPK
Sbjct: 1   MEVALIFITQLIYQNSPIPITSKILISFLLMAFTSILFFQCYNRIRKHSNLQPPLPPGPK 60

Query: 61  PLPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFLKTY 120
           P PLVG LPAMLS NN STHQWIHS+MKQFNTEIA+I L N  IIPV SPELAL+FL+ Y
Sbjct: 61  PWPLVGYLPAMLSSNNFSTHQWIHSIMKQFNTEIASIHLKNKCIIPVISPELALEFLQAY 120

Query: 121 DSVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQRTGE 180
           DSVF SRSSIS+ V++LTRG +STI SP G QW+KM+RIL SEILNPST+ RMLGQRT E
Sbjct: 121 DSVFGSRSSISDDVDMLTRGSVSTILSPTGPQWRKMKRILTSEILNPSTIHRMLGQRTSE 180

Query: 181 ADTLLRYIFTQTNNNEGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEEE 240
           ADTLL YIF QT  N GGAVINVRSITQHYCGNI+RRMLFNRRYYGK REDGGPTFEEEE
Sbjct: 181 ADTLLCYIFNQTCKNGGGAVINVRSITQHYCGNILRRMLFNRRYYGKRREDGGPTFEEEE 240

Query: 241 HNQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQWKK 300
           HNQALLT++R VNAFSISDF+PCLKPFDLDGH K +KNALNVLRKYD+ II++RVQQW+ 
Sbjct: 241 HNQALLTVVRCVNAFSISDFIPCLKPFDLDGHSKILKNALNVLRKYDEPIIEKRVQQWRN 300

Query: 301 DEKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAELINQ 360
           D+KI   EDILDILISLKD N N LLSIEEIK  I D+Q+AT+DNPSNAVEWAMAEL++Q
Sbjct: 301 DKKIKGAEDILDILISLKDDNGNFLLSIEEIKILIIDIQLATVDNPSNAVEWAMAELLDQ 360

Query: 361 PKILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSDIIV 420
           PK+LEKAVEELD+VVGRERLVQESDI KLKYLTAC RESFRLHPFSPFNV HV+  DI++
Sbjct: 361 PKVLEKAVEELDKVVGRERLVQESDILKLKYLTACVRESFRLHPFSPFNVAHVSNYDIVI 420

Query: 421 AGYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGTADLGISEPNLRFITFTRGR 480
           AGYFIPKGSEVLLSRSGLGRNPRIWEDPM+F PERHLKDGT +LGISEP LRFITFTRGR
Sbjct: 421 AGYFIPKGSEVLLSRSGLGRNPRIWEDPMKFDPERHLKDGTVELGISEPTLRFITFTRGR 480

Query: 481 RGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDTDDLSLSKPLHLHAEPRLSHS 540
           RGC GSSLGT+ITMMLFARLLQGFSW+L+P  TKIDL   ++LSLSKPLHLHA+PRLSH 
Sbjct: 481 RGCPGSSLGTSITMMLFARLLQGFSWTLLPKFTKIDLPQPNELSLSKPLHLHAKPRLSHD 540

Query: 541 MY 542
           MY
Sbjct: 541 MY 542

BLAST of Lsi06G005650 vs. ExPASy TrEMBL
Match: A0A6J1D6G9 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498 PE=3 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 2.1e-241
Identity = 420/542 (77.49%), Postives = 474/542 (87.45%), Query Frame = 0

Query: 4   AFLFISQLAHQNSPIAISKTFIVFVFMTFTSIMFSQCYNRRRNHLVRKTPLPPGPKPLPL 63
           A LFISQL +QNSP AI+KTF +FVFM F S +FSQCY +R++ +++   LPPGP P PL
Sbjct: 3   ALLFISQLTYQNSPTAITKTFTLFVFMAFISAIFSQCYRKRKHSVLQ---LPPGPNPWPL 62

Query: 64  VGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFLKTYDSVF 123
           VGCLPAML+   S  ++WIH+VMKQF+TEIA IRLGNTY++PVTSPELA++FLK +DSVF
Sbjct: 63  VGCLPAMLA-TKSPKYKWIHAVMKQFDTEIACIRLGNTYVMPVTSPELAVEFLKRHDSVF 122

Query: 124 ASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQRTGEADTL 183
           ASRS+ISN V +L+RG ++T FSPLG QWKKMRRILASEI  PSTL +MLG RT EADTL
Sbjct: 123 ASRSTISNTVNVLSRGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRTNEADTL 182

Query: 184 LRYIF--TQTNN-NEGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEEEH 243
           LRYIF  T TN  N+ GAV+NVRS+TQHYCGNIIRRMLFN+RYYGKGREDGGP+FEEEEH
Sbjct: 183 LRYIFSLTTTNTVNKDGAVVNVRSVTQHYCGNIIRRMLFNKRYYGKGREDGGPSFEEEEH 242

Query: 244 NQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQWKKD 303
           NQALLTILRHVNAFSISDFMPCLKPFDLDGH+K MK AL  L KYD+ II+ERVQQW KD
Sbjct: 243 NQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKRALKALWKYDEPIINERVQQW-KD 302

Query: 304 EKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAELINQP 363
            K +  EDILDILIS KDGN  SLL+I+EIKAQIT+LQ+ATIDNPSNAVEW MAEL+NQP
Sbjct: 303 GKREGAEDILDILISTKDGNGKSLLTIDEIKAQITELQIATIDNPSNAVEWTMAELLNQP 362

Query: 364 KILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSDIIVA 423
           KIL+KA+EELD VVGRERLVQESDIPKLKYLTACARESFRLHPFSPFN+PHV+ +DI+VA
Sbjct: 363 KILQKAIEELDRVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNIPHVSNADIVVA 422

Query: 424 GYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGTADLGISEPNLRFITFTRGRR 483
           GYFIPKGSEV+LSRSGLGRNPRIWEDPMRF PERHLKD T +LG+SEPNLRFI+FTRGRR
Sbjct: 423 GYFIPKGSEVILSRSGLGRNPRIWEDPMRFDPERHLKDETVELGLSEPNLRFISFTRGRR 482

Query: 484 GCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDTDDLSLSKPLHLHAEPRLSHSM 543
           GC GSSLGTNITMML  RLLQGFSWSL  G TKI+ S+ D+LSL KPLHLHA+PRL H M
Sbjct: 483 GCVGSSLGTNITMMLLGRLLQGFSWSLPFGTTKIEFSEADELSLPKPLHLHAKPRLPHIM 539

BLAST of Lsi06G005650 vs. ExPASy TrEMBL
Match: A0A6J1D6G3 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017495 PE=3 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.6e-239
Identity = 419/543 (77.16%), Postives = 471/543 (86.74%), Query Frame = 0

Query: 4   AFLFISQLAHQNSPI-AISKTFIVFVFMTFTSIMFSQCYNRRRNHLVRKTPLPPGPKPLP 63
           AFL I QL++QNSP  AI+KTF VF+FM F S +FSQCY +R++ +++   LPPGPKP P
Sbjct: 3   AFLIIWQLSYQNSPTGAINKTFTVFLFMAFISAIFSQCYRKRKHSVLQ---LPPGPKPWP 62

Query: 64  LVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFLKTYDSV 123
           LVGCLP+ML+   S  ++WIH+VMKQF+TEIA IRLGNTY+IPVTSPELA++FLK +DSV
Sbjct: 63  LVGCLPSMLA-TKSPKYKWIHAVMKQFDTEIACIRLGNTYVIPVTSPELAVEFLKKHDSV 122

Query: 124 FASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQRTGEADT 183
           FASRS++SN V +LTRG ++T FSPLG QWKKMRRILASEI  PSTL +MLG RT EADT
Sbjct: 123 FASRSTVSNTVNVLTRGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRTNEADT 182

Query: 184 LLRYIF---TQTNNNEGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEEE 243
           LLRYIF   T   NNEGGAV+NV S+TQHYCGNIIRRMLFNRRYYGKGREDGGP+FEEEE
Sbjct: 183 LLRYIFSLTTTNTNNEGGAVVNVGSVTQHYCGNIIRRMLFNRRYYGKGREDGGPSFEEEE 242

Query: 244 HNQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQWKK 303
           HNQALLTILRHVNAFSISDFMPCLKPFDLDGH+K MK AL  L KYD+ II+ERVQQW K
Sbjct: 243 HNQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKRALKALWKYDEPIINERVQQW-K 302

Query: 304 DEKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAELINQ 363
           D K +  EDILDILIS+KDGN  SLL+IEEIKAQIT+LQ+ATIDNPSNAVEW MAEL+NQ
Sbjct: 303 DGKREGAEDILDILISIKDGNGKSLLTIEEIKAQITELQIATIDNPSNAVEWTMAELLNQ 362

Query: 364 PKILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSDIIV 423
           PKIL+KA+EELD VVGR RLVQESDIPKLKYLTA  RESFRLHPFSPFN+PHV+ +DIIV
Sbjct: 363 PKILQKAIEELDRVVGRGRLVQESDIPKLKYLTASVRESFRLHPFSPFNIPHVSNADIIV 422

Query: 424 AGYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGTADLGISEPNLRFITFTRGR 483
           AGYFIPKGSEV+LSRSGLGRNPRIWEDPMRF PERHLKDGT  LG+SEP+LRFI+FTRGR
Sbjct: 423 AGYFIPKGSEVILSRSGLGRNPRIWEDPMRFDPERHLKDGTVALGLSEPSLRFISFTRGR 482

Query: 484 RGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDTDDLSLSKPLHLHAEPRLSHS 543
           RGC GSSLGTNITM L  RLLQGFSWSL  G TKI+ S+ D+LSL KPLHLHA+PRL H 
Sbjct: 483 RGCVGSSLGTNITMTLLGRLLQGFSWSLPFGTTKIEFSEADELSLPKPLHLHAKPRLPHI 540

BLAST of Lsi06G005650 vs. NCBI nr
Match: XP_038880438.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 946.0 bits (2444), Expect = 1.4e-271
Identity = 473/546 (86.63%), Postives = 504/546 (92.31%), Query Frame = 0

Query: 1   MEAAFLFISQLAHQNSPIAI-SKTFIVFVFMTFTSIMFSQCY-NRRRNHLVRKTPLPPGP 60
           M  AFLFI QLAHQNSPIAI SKT IVF FM  TSI+FSQCY NRRR HLVR+ PLPPGP
Sbjct: 1   MVIAFLFIPQLAHQNSPIAITSKTLIVFGFMALTSILFSQCYCNRRRKHLVRRPPLPPGP 60

Query: 61  KPLPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFLKT 120
           KP PLVGCLP ML  N S THQWIHSVMK+ NTEIA+IRLGNTY+IPVTSPELAL+FLKT
Sbjct: 61  KPWPLVGCLPTMLI-NKSPTHQWIHSVMKKLNTEIASIRLGNTYVIPVTSPELALEFLKT 120

Query: 121 YDSVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQRTG 180
           YDSVFASRSS SN + ILTRGY+STI SPLGDQWKKMRRIL S+ILNPSTL RMLGQRT 
Sbjct: 121 YDSVFASRSSTSNAIYILTRGYVSTILSPLGDQWKKMRRILTSKILNPSTLHRMLGQRTA 180

Query: 181 EADTLLRYIFTQTNNNEGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEE 240
           EADTLLRYIFTQTNNN GGAVIN+RSITQHYCGNI+R+MLFNRRYYGKGREDGGPTFEEE
Sbjct: 181 EADTLLRYIFTQTNNNRGGAVINLRSITQHYCGNILRQMLFNRRYYGKGREDGGPTFEEE 240

Query: 241 EHNQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQWK 300
           EH QALLTI++H++AFSISDFMPCLK FDLDGHQK MKNALN+L+KYD+ IIDERVQ+W 
Sbjct: 241 EHVQALLTIVKHIHAFSISDFMPCLKSFDLDGHQKIMKNALNILQKYDEPIIDERVQEW- 300

Query: 301 KDEKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAELIN 360
           KD+KI  VEDILDILISLKD N NSLLSIEEIKAQITDLQ+ATIDNPSNAVEWAMAEL+N
Sbjct: 301 KDKKIKEVEDILDILISLKDENTNSLLSIEEIKAQITDLQIATIDNPSNAVEWAMAELLN 360

Query: 361 QPKILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSDII 420
           QPKILEK VEELD+VVGRERLVQESDIPKLKYLTACARESFRLHPFS FNVPH++TSDI+
Sbjct: 361 QPKILEKVVEELDKVVGRERLVQESDIPKLKYLTACARESFRLHPFSSFNVPHISTSDIV 420

Query: 421 VAGYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGTADLGISEPNLRFITFTRG 480
           VAGYFIPKGSEVLLSRSGLGRNPR+WEDPMRF PERHLKDGT +LGISEPNLRFITFTRG
Sbjct: 421 VAGYFIPKGSEVLLSRSGLGRNPRVWEDPMRFDPERHLKDGTVELGISEPNLRFITFTRG 480

Query: 481 RRGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDTDDLSLSKPLHLHAEPRLSH 540
           RRGCAGSSLGTNITMMLFARLLQGF+WSLM GITKIDLSDT DLSLSKPLHLHA+PRLSH
Sbjct: 481 RRGCAGSSLGTNITMMLFARLLQGFTWSLMAGITKIDLSDTGDLSLSKPLHLHAKPRLSH 540

Query: 541 SMYPKL 545
           SMYP L
Sbjct: 541 SMYPSL 544

BLAST of Lsi06G005650 vs. NCBI nr
Match: XP_038880015.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 901.0 bits (2327), Expect = 5.1e-258
Identity = 452/546 (82.78%), Postives = 496/546 (90.84%), Query Frame = 0

Query: 1   MEAAFLFISQLAHQNSPIAISKTFIVFVFMTFTSIMFSQCYNRRRNHLVRKTPLPPGPKP 60
           MEAAFLFISQLAHQ+SPIAI+KTFIVFVFM FTS++FS  YNRRR   V++ PLPPGPKP
Sbjct: 1   MEAAFLFISQLAHQHSPIAITKTFIVFVFMAFTSVLFSHFYNRRRKLSVQQAPLPPGPKP 60

Query: 61  LPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFLKTYD 120
            PLVGCLP +L+ N S T+QWIHSVMKQFNTEIA+IRLGNTYIIPVTSPELALQFLKTYD
Sbjct: 61  WPLVGCLPTILT-NKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYD 120

Query: 121 SVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQRTGEA 180
           S+FASRSSISN V+IL+RGY++T FS +G+QWKKMRRILASE+LNP++L +MLGQRT EA
Sbjct: 121 SIFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEA 180

Query: 181 DTLLRYIFTQTNNNEGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEEEH 240
           D LL YIF  T+ N GGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPT EEE+H
Sbjct: 181 DNLLCYIFILTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDH 240

Query: 241 NQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQWKKD 300
           N+ALLTILRHVNAFSISDF+PCLKPFDLDGH+K MK ALNV+RKYD+ IIDERVQQW KD
Sbjct: 241 NEALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQW-KD 300

Query: 301 EKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAELINQP 360
           +KI  +EDILDILISLKD + NSLLSIEEIKAQIT+LQ+ATIDNPSNAVEWAMAELINQP
Sbjct: 301 KKIRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQP 360

Query: 361 KILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSDIIVA 420
           +IL+KAVEELDEVVGRERLV+ESDIPKLKYLTAC RESFRLHPFSPFN+PHV+ SDIIV 
Sbjct: 361 EILKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVN 420

Query: 421 GYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGT-ADLGISEPNLRFITFTRGR 480
           GY IPKGSEVLLSR GLGRNPRIWEDPMRF PERHLKD T  +LGISEP+LRFITFTRGR
Sbjct: 421 GYLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGR 480

Query: 481 RGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDTD-DLSLSKPLHLHAEPRLSH 540
           RGC GSSLGTNITMMLFARLLQGFSWSL PGITK+D S+TD  LS+  PL LHA+PRLSH
Sbjct: 481 RGCVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSH 540

Query: 541 SMYPKL 545
           SMYP L
Sbjct: 541 SMYPNL 544

BLAST of Lsi06G005650 vs. NCBI nr
Match: XP_031737864.1 (isoleucine N-monooxygenase 1 [Cucumis sativus])

HSP 1 Score: 894.4 bits (2310), Expect = 4.8e-256
Identity = 438/542 (80.81%), Postives = 481/542 (88.75%), Query Frame = 0

Query: 1   MEAAFLFISQLAHQNSPIAI-SKTFIVFVFMTFTSIMFSQCYNRRRNHLVRKTPLPPGPK 60
           ME A ++I+QL +QN P+AI S TFI+F+FM FTS  F QCYNR   H   + PLPPGPK
Sbjct: 1   MEVALIYITQLIYQNFPVAITSTTFIIFLFMAFTSFFFFQCYNRIGRHTNLQPPLPPGPK 60

Query: 61  PLPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFLKTY 120
           P PLVGCLPAMLSRNNSSTH+WIHS+MKQFN EIA+IRLGNTY+IPVTSPELAL+FLKTY
Sbjct: 61  PWPLVGCLPAMLSRNNSSTHEWIHSIMKQFNVEIASIRLGNTYLIPVTSPELALEFLKTY 120

Query: 121 DSVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQRTGE 180
           DSVF SRSSIS  V++LT G +S I SP G QW+KM+RIL SEILNPSTL R+LGQRT E
Sbjct: 121 DSVFGSRSSISKDVDMLTGGCVSAILSPSGPQWRKMKRILTSEILNPSTLHRVLGQRTAE 180

Query: 181 ADTLLRYIFTQTNNNEGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEEE 240
           AD LL YIF QT  N GGAVINVRSITQHYCGNI+RRM+FNRRYYGKGREDGGPTFEEEE
Sbjct: 181 ADALLHYIFNQTCKNGGGAVINVRSITQHYCGNIVRRMVFNRRYYGKGREDGGPTFEEEE 240

Query: 241 HNQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQWKK 300
           HNQALLTI+RHVN+FSISDFMPCLKPFDLDGHQK MKNALNVL KYD+ II ERVQQWK 
Sbjct: 241 HNQALLTIVRHVNSFSISDFMPCLKPFDLDGHQKIMKNALNVLGKYDERIIKERVQQWKN 300

Query: 301 DEKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAELINQ 360
           D+KI  VEDILDILISL+D + NSLLSIEEIK QI D+Q+ATIDNPSNAVEWAMAEL+NQ
Sbjct: 301 DKKIKGVEDILDILISLEDDDGNSLLSIEEIKTQIMDIQIATIDNPSNAVEWAMAELLNQ 360

Query: 361 PKILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSDIIV 420
           PK+LEKA+EELD+VVGRERLVQESDIPKLKYLT+C RESFRLHPFSPFNV HV+ SDI+V
Sbjct: 361 PKVLEKAIEELDKVVGRERLVQESDIPKLKYLTSCVRESFRLHPFSPFNVAHVSNSDIVV 420

Query: 421 AGYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGTADLGISEPNLRFITFTRGR 480
           AGYFIPKGSEVLLSRSGLGRNPRIWEDPM+F PERHLKDGT +LGISEP LRFITFTRGR
Sbjct: 421 AGYFIPKGSEVLLSRSGLGRNPRIWEDPMKFDPERHLKDGTVELGISEPTLRFITFTRGR 480

Query: 481 RGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDTDDLSLSKPLHLHAEPRLSHS 540
           RGC GSSLGTNITMMLFARLLQGFSW+L+P  TK DL  T++LSLSKPLHLHA+PRLSH 
Sbjct: 481 RGCPGSSLGTNITMMLFARLLQGFSWTLLPKFTKTDLPQTNELSLSKPLHLHAKPRLSHD 540

Query: 541 MY 542
           MY
Sbjct: 541 MY 542

BLAST of Lsi06G005650 vs. NCBI nr
Match: XP_038878794.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 883.6 bits (2282), Expect = 8.5e-253
Identity = 446/547 (81.54%), Postives = 486/547 (88.85%), Query Frame = 0

Query: 1   MEAAFLFISQLAHQNSPIAISKTFIVFVFMTFTSIMFSQCYNRRRNHLV-RKTPLPPGPK 60
           MEAAFL ISQLA+QNS IAISKTFIVFVFM F S++FS CYN +    V RK PLPPGPK
Sbjct: 1   MEAAFLIISQLAYQNSLIAISKTFIVFVFMAFISVIFSHCYNLKTKRSVRRKAPLPPGPK 60

Query: 61  PLPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFLKTY 120
           P PLVGCLP ML+      HQWIHSVMKQFNTEIA+IRLGNTYIIPVTSPELALQFLK Y
Sbjct: 61  PWPLVGCLPTMLT-IKYPPHQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKIY 120

Query: 121 DSVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQRTGE 180
           DS+FASRSSISN V+IL+RGY++T FS +GDQW+KMRRILASEILNP  L RMLGQRT E
Sbjct: 121 DSIFASRSSISNTVDILSRGYLTTAFSSMGDQWRKMRRILASEILNPKMLHRMLGQRTSE 180

Query: 181 ADTLLRYIFTQTNNNEGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEEE 240
           AD+LLRYIFT T+ N GGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEE+
Sbjct: 181 ADSLLRYIFTLTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEED 240

Query: 241 HNQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQWKK 300
           HNQALLTILRHVNAFSISDF+PCLKPFDLDGH+K MK ALN++RKYD+ IIDERVQ WK 
Sbjct: 241 HNQALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKEALNLVRKYDEPIIDERVQHWKV 300

Query: 301 DEKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAELINQ 360
            +KI  +EDILDILISLKD N NSLLSIEEIKAQI +LQ+ATIDNPSNAVEWAMAELINQ
Sbjct: 301 -KKIRVIEDILDILISLKDDNGNSLLSIEEIKAQIVELQIATIDNPSNAVEWAMAELINQ 360

Query: 361 PKILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSDIIV 420
           P++L+KAVEELDEVVGRERLV+ESDIPKLKYLTAC RESFRLHPFSPFN+PHV+ SDI+V
Sbjct: 361 PEVLKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIVV 420

Query: 421 AGYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGT-ADLGISEPNLRFITFTRG 480
            GY IPKGSEVLLSR GLGRNPRIWEDPMRF PERHLKD T  +LGISEP+LRFITFTRG
Sbjct: 421 NGYLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTVTELGISEPSLRFITFTRG 480

Query: 481 RRGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDTD-DLSLSKPLHLHAEPRLS 540
           RRGC GSSLGTNITMMLFARLLQGF WSL+PGITK+D S+ D +LSL  PL L+ +PRLS
Sbjct: 481 RRGCVGSSLGTNITMMLFARLLQGFFWSLLPGITKMDFSEIDHELSLPTPLQLYVQPRLS 540

Query: 541 HSMYPKL 545
           HSMYP L
Sbjct: 541 HSMYPNL 545

BLAST of Lsi06G005650 vs. NCBI nr
Match: TYK27726.1 (isoleucine N-monooxygenase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 878.2 bits (2268), Expect = 3.6e-251
Identity = 432/545 (79.27%), Postives = 474/545 (86.97%), Query Frame = 0

Query: 1   MEAAFLFISQLAHQNSPIA----ISKTFIVFVFMTFTSIMFSQCYNRRRNHLVRKTPLPP 60
           ME A +F  QL +QNSP+A    +  +F++F+FM F SI+F QCYNR   H   + PLPP
Sbjct: 1   MEVASIFTGQLIYQNSPVAMISNLQPSFVMFLFMAFLSILFFQCYNRTEKHSNLQPPLPP 60

Query: 61  GPKPLPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFL 120
           GPKP PL+GCLPAMLS NNSSTH+WIHS+MKQFN EIA+IRL NTY+IPV SPELAL+FL
Sbjct: 61  GPKPWPLLGCLPAMLSSNNSSTHEWIHSIMKQFNAEIASIRLRNTYVIPVASPELALEFL 120

Query: 121 KTYDSVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQR 180
           KTYDSVF SRSS SN V++LT G +STI SP G QW+KM+RIL SEILNPSTL RMLGQR
Sbjct: 121 KTYDSVFGSRSSFSNDVDMLTGGCVSTILSPTGPQWRKMKRILTSEILNPSTLHRMLGQR 180

Query: 181 TGEADTLLRYIFTQTNNNEGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFE 240
           T EAD LL YIF QT  N GGAVINVRSITQHYCGNIIRR++FNRRYYGKGREDGGPTFE
Sbjct: 181 TAEADALLHYIFNQTCKNGGGAVINVRSITQHYCGNIIRRIVFNRRYYGKGREDGGPTFE 240

Query: 241 EEEHNQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQ 300
           EEEHNQALLTI+RHVN+FSISDFMPCLKP DLDGHQK MKNALNVLRKYD+ II ERVQQ
Sbjct: 241 EEEHNQALLTIVRHVNSFSISDFMPCLKPLDLDGHQKNMKNALNVLRKYDEHIIKERVQQ 300

Query: 301 WKKDEKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAEL 360
           WK DEKI  VEDILDILISL D N NSLLSIEEIK QI D+Q+ATIDNPSNAVEWAMAEL
Sbjct: 301 WKNDEKIKGVEDILDILISLTDDNGNSLLSIEEIKNQIMDIQLATIDNPSNAVEWAMAEL 360

Query: 361 INQPKILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSD 420
           +NQPK+L+KA+EELD+VVGRERLVQESDI  LKYLTACARESFRLHPFSPFNV HV+ SD
Sbjct: 361 LNQPKVLKKAIEELDKVVGRERLVQESDISNLKYLTACARESFRLHPFSPFNVAHVSNSD 420

Query: 421 IIVAGYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGTADLGISEPNLRFITFT 480
           I+VAGYFIPKGSEVLLSR GLGRNPRIWEDPM+F PERHLKDGT +LGISEPNLRFITFT
Sbjct: 421 IVVAGYFIPKGSEVLLSRLGLGRNPRIWEDPMKFNPERHLKDGTIELGISEPNLRFITFT 480

Query: 481 RGRRGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDTDDLSLSKPLHLHAEPRL 540
           RGRRGC GSSLGTNITMMLFARLLQGFSW+ +P  TK DL  T++LSL KPLHLHA+PRL
Sbjct: 481 RGRRGCPGSSLGTNITMMLFARLLQGFSWTSLPKFTKTDLPQTNELSLLKPLHLHAKPRL 540

Query: 541 SHSMY 542
           SH MY
Sbjct: 541 SHDMY 545

BLAST of Lsi06G005650 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 518.8 bits (1335), Expect = 5.1e-147
Identity = 254/497 (51.11%), Postives = 358/497 (72.03%), Query Frame = 0

Query: 50  RKTPLPPGPKPLPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSP 109
           R   LPPGPK  PL+G LP +L R N    +WIHS+MK+ NT+IA IRL NT++IPVTSP
Sbjct: 30  RNLSLPPGPKSWPLIGNLPEILGR-NKPVFRWIHSLMKELNTDIACIRLANTHVIPVTSP 89

Query: 110 ELALQFLKTYDSVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTL 169
            +A + LK  DSVFA+R  ++   E  +RGY++    P G+QWKKMRR++AS + +  + 
Sbjct: 90  RIAREILKKQDSVFATR-PLTMGTEYCSRGYLTVAVEPQGEQWKKMRRVVASHVTSKKSF 149

Query: 170 QRMLGQRTGEADTLLRYIFTQTNNNEGGA--VINVRSITQHYCGNIIRRMLFNRRYYGKG 229
           Q ML +RT EAD L+RYI  ++  N G A  VI++R   + Y GN+ R+M+F  R++GKG
Sbjct: 150 QMMLQKRTEEADNLVRYINNRSVKNRGNAFVVIDLRLAVRQYSGNVARKMMFGIRHFGKG 209

Query: 230 REDG-GPTFEEEEHNQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYD 289
            EDG GP  EE EH ++L T+L H+ AF++SD++P L+  DL+GH+K + NA+  + KY+
Sbjct: 210 SEDGSGPGLEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVSNAMRNVSKYN 269

Query: 290 DLIIDERVQQWKKDEKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPS 349
           D  +DER+ QW ++ K+   +D LD+ I  KD +    LS EEIKAQ+T+L +AT+DNPS
Sbjct: 270 DPFVDERLMQW-RNGKMKEPQDFLDMFIIAKDTDGKPTLSDEEIKAQVTELMLATVDNPS 329

Query: 350 NAVEWAMAELINQPKILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSP 409
           NA EW MAE+IN+P I++KAVEE+D VVG++RLV ESD+P L Y+ AC +E+FRLHP +P
Sbjct: 330 NAAEWGMAEMINEPSIMQKAVEEIDRVVGKDRLVIESDLPNLNYVKACVKEAFRLHPVAP 389

Query: 410 FNVPHVATSDIIVAGYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGTADLGIS 469
           FN+PH++T+D +V GYFIPKGS VL+SR G+GRNP +W+ P +F PERHL   T  + ++
Sbjct: 390 FNLPHMSTTDTVVDGYFIPKGSHVLISRMGIGRNPSVWDKPHKFDPERHLSTNTC-VDLN 449

Query: 470 EPNLRFITFTRGRRGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDT-DDLSLS 529
           E +L  I+F+ GRRGC G  +G+ +T ML ARL+QGF+W  +PG  KID+S++ +DL ++
Sbjct: 450 ESDLNIISFSAGRRGCMGVDIGSAMTYMLLARLIQGFTWLPVPGKNKIDISESKNDLFMA 509

Query: 530 KPLHLHAEPRLSHSMYP 543
           KPL+  A PRL+  +YP
Sbjct: 510 KPLYAVATPRLAPHVYP 522

BLAST of Lsi06G005650 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 488.0 bits (1255), Expect = 9.6e-138
Identity = 248/528 (46.97%), Postives = 360/528 (68.18%), Query Frame = 0

Query: 19  AISKTFIVFVFMTFTSIMFSQCYNRRRNHLVRKT--PLPPGPKPLPLVGCLPAMLSRNNS 78
           + +  +++       ++ F    N+ ++    K   PLPPGP   P+VG +PAML   N 
Sbjct: 21  SFTNMYLLTTLQALAALCFLMILNKIKSSSRNKKLHPLPPGPTGFPIVGMIPAML--KNR 80

Query: 79  STHQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFLKTYDSVFASRSSISNVVEIL 138
              +W+HS+MK+ NTEIA +RLGNT++IPVT P++A +  K  D++FASR  ++   +IL
Sbjct: 81  PVFRWLHSLMKELNTEIACVRLGNTHVIPVTCPKIAREIFKQQDALFASR-PLTYAQKIL 140

Query: 139 TRGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQRTGEADTLLRYIFTQTNNNEG 198
           + GY + + +P G+Q+KKMR+++ +EI+ P+  + +   R  E D L  +++    N+E 
Sbjct: 141 SNGYKTCVITPFGEQFKKMRKVIMTEIVCPARHRWLHDNRAEETDHLTAWLYNMVKNSE- 200

Query: 199 GAVINVRSITQHYCGNIIRRMLFNRRYYGKGRE-DGGPTFEEEEHNQALLTILRHVNAFS 258
              +++R +T+HYCGN I+R++F  R + +  E DGGPT E+ EH  A+   L    AF 
Sbjct: 201 --PVDLRFVTRHYCGNAIKRLMFGTRTFSEKTEADGGPTLEDIEHMDAMFEGLGFTFAFC 260

Query: 259 ISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQWKKDEKIDRVEDILDILIS 318
           ISD++P L   DL+GH+K M+ +  ++ KY D IIDER++ W++ ++  ++ED LDI IS
Sbjct: 261 ISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDERIKMWREGKR-TQIEDFLDIFIS 320

Query: 319 LKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAELINQPKILEKAVEELDEVVG 378
           +KD     LL+ +EIK  I +L +A  DNPSNAVEWA+AE+IN+P+IL KA+EE+D VVG
Sbjct: 321 IKDEAGQPLLTADEIKPTIKELVMAAPDNPSNAVEWAIAEMINKPEILHKAMEEIDRVVG 380

Query: 379 RERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSDIIVAGYFIPKGSEVLLSRS 438
           +ER VQESDIPKL Y+ A  RE+FRLHP + FN+PHVA SD  VAGY IPKGS+VLLSR 
Sbjct: 381 KERFVQESDIPKLNYVKAIIREAFRLHPVAAFNLPHVALSDTTVAGYHIPKGSQVLLSRY 440

Query: 439 GLGRNPRIWEDPMRFGPERHLKDGTADLGISEPNLRFITFTRGRRGCAGSSLGTNITMML 498
           GLGRNP++W DP+ F PERHL +  +++ ++E +LRFI+F+ G+RGCA  +LGT IT M+
Sbjct: 441 GLGRNPKVWSDPLSFKPERHLNE-CSEVTLTENDLRFISFSTGKRGCAAPALGTAITTMM 500

Query: 499 FARLLQGFSWSLMPGITKIDLSDTD-DLSLSKPLHLHAEPRLSHSMYP 543
            ARLLQGF W L    T+++L ++  D+ LSKPL L  E RLS  +YP
Sbjct: 501 LARLLQGFKWKLAGSETRVELMESSHDMFLSKPLVLVGELRLSEDLYP 540

BLAST of Lsi06G005650 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 478.0 bits (1229), Expect = 1.0e-134
Identity = 241/527 (45.73%), Postives = 359/527 (68.12%), Query Frame = 0

Query: 19  AISKTFIVFVFMTFTSIMFSQCYNRRRNHLVRKTP-LPPGPKPLPLVGCLPAMLSRNNSS 78
           + S  +++     F +I       +      +K P LPPGP   P++G +P ML   +  
Sbjct: 20  SFSTLYLLSTLQAFVAITLVMLLKKLMTDPNKKKPYLPPGPTGWPIIGMIPTML--KSRP 79

Query: 79  THQWIHSVMKQFNTEIATIRLGNTYIIPVTSPELALQFLKTYDSVFASRSSISNVVEILT 138
             +W+HS+MKQ NTEIA ++LGNT++I VT P++A + LK  D++FASR  ++   +IL+
Sbjct: 80  VFRWLHSIMKQLNTEIACVKLGNTHVITVTCPKIAREILKQQDALFASR-PLTYAQKILS 139

Query: 139 RGYISTIFSPLGDQWKKMRRILASEILNPSTLQRMLGQRTGEADTLLRYIFTQTNNNEGG 198
            GY + + +P GDQ+KKMR+++ +E++ P+  + +  +R+ E D L  +++    N+   
Sbjct: 140 NGYKTCVITPFGDQFKKMRKVVMTELVCPARHRWLHQKRSEENDHLTAWVYNMVKNS--- 199

Query: 199 AVINVRSITQHYCGNIIRRMLFNRRYYGKG-REDGGPTFEEEEHNQALLTILRHVNAFSI 258
             ++ R +T+HYCGN I++++F  R + K    DGGPT E+ EH +A+   L    AF I
Sbjct: 200 GSVDFRFMTRHYCGNAIKKLMFGTRTFSKNTAPDGGPTVEDVEHMEAMFEALGFTFAFCI 259

Query: 259 SDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLIIDERVQQWKKDEKIDRVEDILDILISL 318
           SD++P L   DL+GH+K M+ +  ++ KY D IIDER++ W++ ++  ++ED LDI IS+
Sbjct: 260 SDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDERIKMWREGKR-TQIEDFLDIFISI 319

Query: 319 KDGNANSLLSIEEIKAQITDLQVATIDNPSNAVEWAMAELINQPKILEKAVEELDEVVGR 378
           KD   N LL+ +EIK  I +L +A  DNPSNAVEWAMAE++N+P+IL KA+EE+D VVG+
Sbjct: 320 KDEQGNPLLTADEIKPTIKELVMAAPDNPSNAVEWAMAEMVNKPEILRKAMEEIDRVVGK 379

Query: 379 ERLVQESDIPKLKYLTACARESFRLHPFSPFNVPHVATSDIIVAGYFIPKGSEVLLSRSG 438
           ERLVQESDIPKL Y+ A  RE+FRLHP + FN+PHVA SD  VAGY IPKGS+VLLSR G
Sbjct: 380 ERLVQESDIPKLNYVKAILREAFRLHPVAAFNLPHVALSDTTVAGYHIPKGSQVLLSRYG 439

Query: 439 LGRNPRIWEDPMRFGPERHLKDGTADLGISEPNLRFITFTRGRRGCAGSSLGTNITMMLF 498
           LGRNP++W DP+ F PERHL +  +++ ++E +LRFI+F+ G+RGCA  +LGT +T M+ 
Sbjct: 440 LGRNPKVWADPLCFKPERHLNE-CSEVTLTENDLRFISFSTGKRGCAAPALGTALTTMML 499

Query: 499 ARLLQGFSWSLMPGITKIDLSDTD-DLSLSKPLHLHAEPRLSHSMYP 543
           ARLLQGF+W L    T+++L ++  D+ L+KPL +  + RL   +YP
Sbjct: 500 ARLLQGFTWKLPENETRVELMESSHDMFLAKPLVMVGDLRLPEHLYP 538

BLAST of Lsi06G005650 vs. TAIR 10
Match: AT5G35917.1 (cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene )

HSP 1 Score: 437.6 bits (1124), Expect = 1.5e-122
Identity = 229/499 (45.89%), Postives = 325/499 (65.13%), Query Frame = 0

Query: 50  RKTPLPPGPKPLPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSP 109
           R   LPPGPK  PLVG LP +L R N    +WIHS+M++ NT+IA IRL NT++IPVTSP
Sbjct: 38  RNLSLPPGPKSWPLVGNLPEILGR-NKPVFRWIHSLMEELNTDIACIRLANTHVIPVTSP 97

Query: 110 ELALQFLKTYDSVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTL 169
            +A + LK  DSVFA R  ++   E  +RGY++      G+QWKKMRR++AS + +  + 
Sbjct: 98  RIAREILKKQDSVFAIR-PLTMGTEYCSRGYLTIAVQSQGEQWKKMRRVVASHVTSKKSF 157

Query: 170 QRMLGQRTGEADTLLRYIFTQTNNNEGG----AVINVRSITQHYCGNIIRRMLFNRRYYG 229
           + ML +RT EAD L+RYI  +   N G     AVI++R + + Y GN+ R+M+F  R++G
Sbjct: 158 KLMLEKRTEEADNLVRYINNRCVKNRGNGNGLAVIDLRFVVRQYSGNVARKMMFGIRHFG 217

Query: 230 KGRED-GGPTFEEEEHNQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRK 289
           KG ED  GP  EE EH ++L T+L H+ AF++SD++P L+  DL+GH+K + +A+  + K
Sbjct: 218 KGSEDESGPGSEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVADAMRNVSK 277

Query: 290 YDDLIIDERVQQWKKDEKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDN 349
           Y+D  +DER+ QW ++ K+   +D LD+ I  KD N    LS EEIKAQ+T+L +AT+DN
Sbjct: 278 YNDPFVDERLMQW-RNGKMKEPQDFLDMFIMAKDTNGKPTLSEEEIKAQVTELMLATVDN 337

Query: 350 PSNAVEWAMAELINQPKILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPF 409
           PSNA EWAMAE+IN+P I++KAVEE+D    RE + +               E+FRLHP 
Sbjct: 338 PSNAAEWAMAEMINEPSIMQKAVEEIDRSNLRESVCE---------------ETFRLHPV 397

Query: 410 SPFNVPHVATSDIIVAGYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGTADLG 469
           +PFN+PH++T+D +V GYFIPKGS +L+SR G+GRNP +W+ P++F PERHL +      
Sbjct: 398 APFNLPHMSTADAVVDGYFIPKGSHMLISRMGIGRNPNVWDKPLKFNPERHLGN------ 457

Query: 470 ISEPNLRFITFTRGRRGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDLSDT-DDLS 529
                                   +++T ML ARL+QGF+WS +PG +KID+S++  DL 
Sbjct: 458 -----------------------NSSMTYMLLARLIQGFTWSPVPGESKIDISESKSDLF 489

Query: 530 LSKPLHLHAEPRLSHSMYP 543
           ++KPLH  A PRL   +YP
Sbjct: 518 MAKPLHAVATPRLDPHVYP 489

BLAST of Lsi06G005650 vs. TAIR 10
Match: AT1G58260.1 (cytochrome p450 79c2 )

HSP 1 Score: 408.7 bits (1049), Expect = 7.4e-114
Identity = 210/499 (42.08%), Postives = 327/499 (65.53%), Query Frame = 0

Query: 51  KTPLPPGPKPLPLVGCLPAMLSRNNSSTHQWIHSVMKQFNTEIATIRLGNTYIIPVTSPE 110
           K  LPPGP+  P+VG +  M+   N   H WIH VM++  T+IA  R    ++I VTS +
Sbjct: 34  KGQLPPGPRGWPIVGNMLQMII--NRPAHLWIHRVMEELQTDIACYRFARFHVITVTSSK 93

Query: 111 LALQFLKTYDSVFASRSSISNVVEILTRGYISTIFSPLGDQWKKMRRILASEILNPSTLQ 170
           +A + L+  D V A RS  S    +++ GY +  FS  G+ WK +++++ +++++P+TL 
Sbjct: 94  IAREVLREKDEVLADRSE-SYASHLISHGYKNISFSSYGENWKLVKKVMTTKLMSPTTLS 153

Query: 171 RMLGQRTGEADTLLRYIFTQTNNNEGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGRED 230
           + LG R  EAD ++ Y++           INVR     YC  ++ RM+F +R++ +  E+
Sbjct: 154 KTLGYRNIEADNIVTYVYNLCQLGSVRKPINVRDTILTYCHAVMMRMMFGQRHFDEVVEN 213

Query: 231 GGPTFEEEEHNQALLTILRHVNAFSISDFMPCLKPFDLDGHQKTMKNALNVLRKYDDLII 290
           GG   +E+EH  A+   L    +F++++++P L+ +++D  +  ++ A++++   +D II
Sbjct: 214 GGLGPKEKEHMDAIYLALDCFFSFNLTNYIPFLRGWNVDKAETEVREAVHIINICNDPII 273

Query: 291 DERVQQWKKDEKIDRVEDILDILISLKDGNANSLLSIEEIKAQITDLQVATIDNPSNAVE 350
            ER+  W+K       ED LDILI+LKD     L + +EI+AQ  ++ +ATIDN  N VE
Sbjct: 274 QERIHLWRKKGGKQMEEDWLDILITLKDDQGMHLFTFDEIRAQCKEINLATIDNTMNNVE 333

Query: 351 WAMAELINQPKILEKAVEELDEVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNVP 410
           W +AE++N P+ILEKA  ELD +VG++RLVQESDI +L Y+ AC++ESFRLHP + F   
Sbjct: 334 WTIAEMLNHPEILEKATNELDIIVGKDRLVQESDISQLNYIKACSKESFRLHPANVFMPH 393

Query: 411 HVATSDIIVAGYFIPKGSEVLLSRSGLGRNPRIWEDPMRFGPERHLKDGTAD--LGIS-- 470
           HVA  D  +AGYF+PKGS++L+SR GLGRNP+IW++P  F PER+L DG  +  LG++  
Sbjct: 394 HVAREDTTLAGYFVPKGSQILVSRLGLGRNPKIWDEPNAFKPERYL-DGHVEKSLGVTLM 453

Query: 471 EPNLRFITFTRGRRGCAGSSLGTNITMMLFARLLQGFSWSLMPGITKIDL-SDTDDLSLS 530
           EP++RF+TF  GRR C G+ +GT++T+ML ARL+QGF W+L  G + ++L S   +L ++
Sbjct: 454 EPDMRFVTFGTGRRSCPGTKIGTSMTIMLLARLIQGFEWTLPIGKSSVELISAESNLFMA 513

Query: 531 KPLHLHAEPRLSHSMYPKL 545
           KPL   A+PRL+ S+YPK+
Sbjct: 514 KPLLACAKPRLAPSLYPKI 528

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A068Q6051.3e-16051.38Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV... [more]
Q6J5416.5e-15552.57Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1[more]
Q6J5402.7e-15351.04Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1[more]
Q431352.0e-14851.20Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3[more]
A0A068Q7V07.2e-14649.70Phenylalanine N-monooxygenase CYP79D16 OS=Prunus mume OX=102107 GN=CYP79D16 PE=1... [more]
Match NameE-valueIdentityDescription
A0A5D3DVF91.7e-25179.27Isoleucine N-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3BQ376.5e-25179.85isoleucine N-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103491994 PE=3 S... [more]
A0A1S3BNG97.0e-24578.60isoleucine N-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103491996 PE=3 S... [more]
A0A6J1D6G92.1e-24177.49isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498... [more]
A0A6J1D6G32.6e-23977.16isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017495... [more]
Match NameE-valueIdentityDescription
XP_038880438.11.4e-27186.63tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida][more]
XP_038880015.15.1e-25882.78tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida][more]
XP_031737864.14.8e-25680.81isoleucine N-monooxygenase 1 [Cucumis sativus][more]
XP_038878794.18.5e-25381.54tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida][more]
TYK27726.13.6e-25179.27isoleucine N-monooxygenase 1-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT5G05260.15.1e-14751.11cytochrome p450 79a2 [more]
AT2G22330.19.6e-13846.97cytochrome P450, family 79, subfamily B, polypeptide 3 [more]
AT4G39950.11.0e-13445.73cytochrome P450, family 79, subfamily B, polypeptide 2 [more]
AT5G35917.11.5e-12245.89cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene [more]
AT1G58260.17.4e-11442.08cytochrome p450 79c2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 433..457
score: 30.3
coord: 116..135
score: 22.46
coord: 349..375
score: 35.49
coord: 472..482
score: 44.74
coord: 392..410
score: 33.93
coord: 329..346
score: 22.34
coord: 482..505
score: 29.73
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 47..540
e-value: 1.1E-101
score: 342.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 55..535
IPR001128Cytochrome P450PFAMPF00067p450coord: 55..525
e-value: 8.3E-79
score: 265.5
NoneNo IPR availablePIRSRPIRSR000209-1PIRSR000209-1coord: 56..507
e-value: 5.6E-28
score: 95.0
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 29..540
NoneNo IPR availablePANTHERPTHR24298:SF675PHENYLALANINE N-MONOOXYGENASEcoord: 29..540

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G005650.1Lsi06G005650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen