Lsi06G005620 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G005620
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	isoleucine N-monooxygenase 1-like
Location	chr06: 7176065 .. 7180539 (-)
RNA-Seq Expression	Lsi06G005620
Synteny	Lsi06G005620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGTTAATATATTTAAATTTTTATTTATTTATATTTAATCCAAATTTAAAACGTCAATGACAACCTATATTCCCACCTATAGTTTTGTTTAATTCACTATATATTTTTTAATTATTATTATTTTTTGACATTATCATAGTGTAATAGATGTAATATCTGCCCATAGTTTGTTTTATATATATATATATATATATATATATATATATATAGTGAATAAGTTGTGATCCTCAACCCATGTTATTTACTTTACTAGATTTTTTTATATATTTTTTAAAATGTATCTAGGGTTTAAGGGAGGTTAGATCGGGGAACATAATTCTCGATATTATAGTATTTTATAATGATTTAGTACTAATTTATCAAATATAATAATATGAATTTTTTTAAACTTAAAAAACACACTAGATTTTCTTAAGGAGAATTTTTAAAAATAAAAAAATAAAGGAAACTATTTATATAAAATAGCAAAATTTTTAGATAGTTGTGATAGACGCTGATAGAAACCTATCAGGATCTATCAACAATAGAAATGATAGAAGTCTATCACTGATAGATATGCTGATAGAAGTCTATTACTGATAGATGTGTCTATCAGTATTTTTTTGTTTTTTTCTATAAATAGTTTGACATTTTTTTTATTTGTGAAATTTTTCCATTTCTTAAAAGAAAAGCTGACTAAACATTAATTATTTTAACGGTTACAATAATTTTAAATTAAGTCTGAATTTTGTTAGAAAAATAAAAAAATAAAAAAACCTGAATCTTCCCCTCATTAAGGCTGGAATGCAAATGTTTTGCGATTTTTCCATGCTACTTCCACAATTTTATAACGTAAAAAAAAAAAAAAGTAGAAAATTTTAACAAATCAAAATTTTAAAAACTGTGGTCGGTAAAGTTGCAGAATATTTTAAATTTGATAGGCACAAATATATATAAAGAAATAAAAATTTAAAATATTTAATCACTGTACCTAAAATATTACATGAAAACAAACAAGACATTGACTTCTATTTTTAATTTTTATTTTTGAAATAAACATTGACTTCTATTTTTCCTACATTTGATACTAGACTTCTAGTAATCGGTAATCTATTTCCTATCATGATGTCATTAAATAATATATTATTTTATAGAGAATTTTTAAAAATAGAGGAATGAGAAAAATTATTTATACAAAATTGCAAAATTTTTAGATAGTTGTGATAGATGTCATAGAAGTCTATGAAGGTCTATCAATGATAAAAATGATAGAAGTCTATCACTAATAGATGTTAATAGAAGTCTATCAGTATTTTTTTTTGCTATTTTCTATAAATAATTTGACATTTTTTTATCTGTAAATTTTTTATTTTATTTTATTTTATAAAGCATTATATAATAAAACTTTTAATAAAATAAAAATAAATTAAGTTTATAACATGAAATAATTTATTTATTTATTATTAATAAAAATTTTTTTTACTAGTATATACGTAATGTCATTCCAACATATTGGCCCTACGGTCATTTATAAAATTGAAAATTGCAAACTTCAAGGAAATTGAATTTCTTAACCCAAAAAATACCGTTTTAGAAAAGTATTTGGGAAAAATAGTAATGTTTCCTTATTATTAAAGCTTCTCTATATATATAATTTAGAAAATTATTTGATTAGGTTGTATCCATCTTTTTGTAAATTAGTTGGTCTAACTACAATTTGTTATCACGTGGTAAACTCATAATGATTTTTTTTAAAAATATATTTTTAAACTTTTTCTAATGTGTGATTTAAGTGATATTAAATCAAATGAGTGTCGTCCAAGATATACCCAAATATTGCTCGATATTTAGAAAATTCATGCATACCATTTGTTTGAGTTGCTCTAAAAAGTAACAACTTGGTTGGCAGGCAATCTAGGTTATATTTTATGTTTTCATAATCATTTCAACAAAAAAGAGTTTGGTTAGCTATCTCGATTGTGCTTTTTAAAATTGTTTTTGAATTTTGTGATTTAAATAGTGCAAATTTTGTAAACATCATTTTTAAGTTTTCAATATATCCAAATTTCATATTTAAAATTTTAAAATGCAGAAATATTTTAAAAAAATATAAGGATGTTAATACATAGGGATAATTATAGATGTATATCCTCTACAAAACTTATTTTCATATAAATATCTAAATTTTGGATAAAAAAATATCTAAATTTTGGATATTTTTATTTTGAGGATTTAATTGAAAAATAGAACAAAAATACCCTTTTTCTTAGTAGATGACGTGCATTTATTGCTATTTATTAGGCAAAACTTTTCCATATTTTTCAAACATTCTTAACGATGGAGAGAGAATTTGACCAAATATATATTAATTAGTGTGTTGGAAGGAGAATATACATCTATTATAAATTATTAATTGATTTGAGTTTTTTTTTTCATTTACAACTAATGTGATTTTTTTTCTCATTTTCATGTATGAAACATTTTTTGCTTTTTCTTTCTTCTCGTGGATTTATGGTTATATTTTCAATATACTTTAGAACATTGTTTTAGGTGTTTGAAAATATTCTAACCAATATATGAAATATACCGCAGTAAATAAATGAGAAATGACTCAATGTTCAACATAAATCACAATATAAATCATAAATTTCATTATATCACAGTCTATATAATCTCAATAAGTCAAAATTTAACAACAACTCCTTAACAGAAATGTAATTCAAAATTAATGTAATAACTTCAATGTACCACAGTATATAAATCACTCAACGTTCAACATAAATCACAGTATATATCATAAATTTAATTTATATCACAGTATATATCATAAATTCCATTTAAGTAGTTAGAAATTCCAATATATATCCTATATATTTAGGATTTAAAAAAATAAAAGAGGAAAAAGTATCATCGGTTTGTGTGCATATAACCCTCCCATCTTCTTCTTCCTCGTGAAGCCATTGTCGTCATTCATGCTCTTTGGTTTGACCGTCCGTCGCGCCAACCGTCACGCATCCACCATCGTTTGAGTTGCGCCGTCATTCATAGTCAATTCGAGTCACGAACCGTCCGTCGTTTGTCGTCCTCCTCTCGCGCACCGTCCATCGTCCATTTTCTTTGCACCAGCTTCTTCATCGTCGTCTGCTCGCTAGTGTGGTCACCGGTGAAGATTTTTGTTGTGGATTTTCATTTTGCAGCGGATTTAGGCGGAGGAGGATCTGTGCAGAGGAGGACCTAAGAGAGACGTTGGAGAAGATGAAAAGTGAATGGGTATTTGGGGTTTAATTCAATTTCTTATTTTAAAAAGCATGTAACTAATTGATGGTTGGGGACCGAATGGTAATTGAACCCTAATTTTTGTGTAATTTTAAGCCAACTTAAAGATTTTTGTGTAATTTTAAGCCAACTTAAACATTCCGGTAAATAATAGAAAAGTTGAACATAATTGAAATATAGAGCTTTTATTAGACCTTATGTGTTGTTATCCCTTAGAAATAAGGGATTTTTATATAGAAGGGCTAAAAATAGGTCCATATTACGTTCATATCTTTTACATAAAATAAACTTTCAAATTAATACATGGTTAATACATATGGCTAAATAATCATCCAAAAACTTAACTTAATATTACAAATAATGTTATCCATTCTTTAAACTAATAATATTTATTTTTATTAGATATAAAATTGAATTTTATGTCTAAATAGAAGAACTTCCAAATTCACGTTTGGTGGGTTTTAATTTTATAAACTGTTGAAAAGCTCAAAGGTCATCTATTGACACAATTAAAATTCAAAGGCTCAATCATTATTGGTCATTAGAAGTTACATAGATTTTCAAGCATCAACTTTCTCATGGATATGTTTTTTCTTCTACCAAAAAAGTTTCGTATCAATCAAAATAGTTGCGCATCTCTTATATATATACTCATGATCTTCTCCTTATTTATAATAAACGTGAGACTTTGGTCGCTTTTTCCAACAATGATAAATTAAATATTTTTTTTTTGTCTCTTTACATATGCCACCAATGTATATATATAAACTCATATCCCCTAAAGGATATAGGTATACAAAGCATTCCACTACTACAAATAGTGAGAGTGAACGAGAAAGGGAGTGAGAGAAAACCATGGAAGTTTAATTTCTTTCTTATTCATTAGTCAATTGGCACACCAAAACTCTCCAGTAGCCATATCAGAAACTTTCATTGTGTTTGTATTTATGGCTTTCATTTCTATGATACTTTTCCAAAGTTATAACCTCAAAAGAAAACACTCAGTTCAACGACAAGCACCTCTCCCACCAGGCCCAAAGCCATGGCCACTCGTAGGTTGCCTCCCAACAATGTTAACCAACAAAATCCCAACACACCAATGGATTCATTCAGTAATGAAGCAATTCAACACTGAAATTGCATCCATACGCCTTGGAAATACTCACATAATCCCTATCACTTCTCCAGAACTCGCTCGGGAATTTCTTAAGACATGA

mRNA sequence

ATGACTCAATTGGCACACCAAAACTCTCCAGTAGCCATATCAGAAACTTTCATTGTGTTTGTATTTATGGCTTTCATTTCTATGATACTTTTCCAAAGTTATAACCTCAAAAGAAAACACTCAGTTCAACGACAAGCACCTCTCCCACCAGGCCCAAAGCCATGGCCACTCGTAGGTTGCCTCCCAACAATGTTAACCAACAAAATCCCAACACACCAATGGATTCATTCAGTAATGAAGCAATTCAACACTGAAATTGCATCCATACGCCTTGGAAATACTCACATAATCCCTATCACTTCTCCAGAACTCGCTCGGGAATTTCTTAAGACATGA

Coding sequence (CDS)

ATGACTCAATTGGCACACCAAAACTCTCCAGTAGCCATATCAGAAACTTTCATTGTGTTTGTATTTATGGCTTTCATTTCTATGATACTTTTCCAAAGTTATAACCTCAAAAGAAAACACTCAGTTCAACGACAAGCACCTCTCCCACCAGGCCCAAAGCCATGGCCACTCGTAGGTTGCCTCCCAACAATGTTAACCAACAAAATCCCAACACACCAATGGATTCATTCAGTAATGAAGCAATTCAACACTGAAATTGCATCCATACGCCTTGGAAATACTCACATAATCCCTATCACTTCTCCAGAACTCGCTCGGGAATTTCTTAAGACATGA

Protein sequence

MTQLAHQNSPVAISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT

Homology

[Close]

BLAST of Lsi06G005620 vs. ExPASy Swiss-Prot
Match: A0A068Q605 (Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 4.1e-24
Identity = 55/101 (54.46%), Postives = 75/101 (74.26%), Query Frame = 0


Query: 10  PVAISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKI 69
           P+++S  FI+F+F+      + +++N   K+SV    PLPPGP PWP+VG LP M  N+ 
Sbjct: 15  PISLSLAFIIFMFLV---KFILKTHN---KNSVP-VVPLPPGPSPWPIVGSLPEMWRNR- 74

Query: 70  PTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
           P H+WIHS+MK+ NT+IA IRLGN H+IP+TSPE+AREFLK
Sbjct: 75  PAHRWIHSLMKKLNTDIACIRLGNVHVIPVTSPEIAREFLK 107

[Close]

BLAST of Lsi06G005620 vs. ExPASy Swiss-Prot
Match: Q9FLC8 (Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.6e-20
Identity = 49/95 (51.58%), Postives = 62/95 (65.26%), Query Frame = 0


Query: 23  MAFISMILFQSYNLKRKH-------SVQRQAPLPPGPKPWPLVGCLPTMLTNKIPTHQWI 82
           +AFI  +L  +  +KRK        S  R   LPPGPK WPL+G LP +L    P  +WI
Sbjct: 8   LAFIIGLLLLALTMKRKEKKKTMLISPTRNLSLPPGPKSWPLIGNLPEILGRNKPVFRWI 67

Query: 83  HSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
           HS+MK+ NT+IA IRL NTH+IP+TSP +ARE LK
Sbjct: 68  HSLMKELNTDIACIRLANTHVIPVTSPRIAREILK 102

[Close]

BLAST of Lsi06G005620 vs. ExPASy Swiss-Prot
Match: O81346 (Tryptophan N-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP79B2 PE=1 SV=2)

HSP 1 Score: 92.4 bits (228), Expect = 3.4e-18
Identity = 41/99 (41.41%), Postives = 65/99 (65.66%), Query Frame = 0


Query: 12  AISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKIPT 71
           + S  +++    AF+++ L            +++  LPPGP  WP++G +PTML ++ P 
Sbjct: 20  SFSTLYLLSTLQAFVAITLVMLLKKLMTDPNKKKPYLPPGPTGWPIIGMIPTMLKSR-PV 79

Query: 72  HQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
            +W+HS+MKQ NTEIA ++LGNTH+I +T P++ARE LK
Sbjct: 80  FRWLHSIMKQLNTEIACVKLGNTHVITVTCPKIAREILK 117

[Close]

BLAST of Lsi06G005620 vs. ExPASy Swiss-Prot
Match: Q6J540 (Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 3.4e-18
Identity = 48/97 (49.48%), Postives = 65/97 (67.01%), Query Frame = 0


Query: 14  SETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKIPTHQ 73
           S TF++ V   FI   + +S+++ +     ++  LPPGPKPWP+VG LP ML N+ P   
Sbjct: 15  SWTFLLVVIFFFIIFKVTKSHSVNK----SKKYKLPPGPKPWPIVGNLPEMLANR-PATI 74

Query: 74  WIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
           WIH +MK+ NTEIA IRL NT +IP+T P +A EFLK
Sbjct: 75  WIHKLMKEMNTEIACIRLANTIVIPVTCPTIACEFLK 106

[Close]

BLAST of Lsi06G005620 vs. ExPASy Swiss-Prot
Match: O81345 (Cytochrome P450 79B1 OS=Sinapis alba OX=3728 GN=CYP79B1 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 2.9e-17
Identity = 43/103 (41.75%), Postives = 70/103 (67.96%), Query Frame = 0


Query: 11  VAISETFIVFVFMAFISMILFQSYNLKR---KHSVQRQAPLPPGPKPWPLVGCLPTMLTN 70
           ++ S  +++    AF+++ L     LK+     + +++  LPPGP  WP++G +PTML +
Sbjct: 19  LSFSNMYLLTTLQAFVAITLVML--LKKVLVNDTNKKKLSLPPGPTGWPIIGMVPTMLKS 78

Query: 71  KIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
           + P  +W+HS+MKQ NTEIA +RLG+TH+I +T P++ARE LK
Sbjct: 79  R-PVFRWLHSIMKQLNTEIACVRLGSTHVITVTCPKIAREVLK 118

[Close]

BLAST of Lsi06G005620 vs. ExPASy TrEMBL
Match: A0A1S3BQ37 (isoleucine N-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103491994 PE=3 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.3e-40
Identity = 82/111 (73.87%), Postives = 99/111 (89.19%), Query Frame = 0


Query: 1   MTQLAHQNSPVAISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGC 60
           +TQL HQNSP+AIS+ FIVF+FMAF S++  + YN ++K+SV R+A LPPGPKPWP+VGC
Sbjct: 9   ITQLPHQNSPIAISKAFIVFLFMAFASVMFSRCYNRRKKYSV-RKALLPPGPKPWPIVGC 68

Query: 61  LPTMLTNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           LPTMLTNK PT+QWIHSVMKQFNTEIA IRLGNT++IP+TSPELA EFL+T
Sbjct: 69  LPTMLTNKSPTYQWIHSVMKQFNTEIACIRLGNTYVIPVTSPELALEFLRT 118

[Close]

BLAST of Lsi06G005620 vs. ExPASy TrEMBL
Match: A0A0A0LXR0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G620390 PE=4 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.2e-39
Identity = 80/111 (72.07%), Postives = 96/111 (86.49%), Query Frame = 0


Query: 1   MTQLAHQNSPVAISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGC 60
           +TQL HQ+SP+AIS+TFI+F+FMAF S++    YN ++K+SV R+  LPPGPKPWP+VGC
Sbjct: 9   LTQLTHQHSPIAISKTFIIFLFMAFASVMFSHYYNRRKKYSVVRKPRLPPGPKPWPIVGC 68

Query: 61  LPTMLTNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           LPTMLTNK PT  WIHSVMKQFNTEIA IRLG+T+IIP+TSPELA EFLKT
Sbjct: 69  LPTMLTNKSPTSHWIHSVMKQFNTEIACIRLGSTYIIPVTSPELAIEFLKT 119

[Close]

BLAST of Lsi06G005620 vs. ExPASy TrEMBL
Match: A0A0A0LXG7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G618390 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 4.6e-34
Identity = 77/113 (68.14%), Postives = 90/113 (79.65%), Query Frame = 0


Query: 1   MTQLAHQNSPVAI-SETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVG 60
           +TQL +QN PVAI S TFI+F+FM+F S   FQ YN   +H+   Q PLPPGPKPWPLVG
Sbjct: 8   ITQLIYQNFPVAITSTTFIIFLFMSFTSFFFFQCYNRIGRHT-NLQPPLPPGPKPWPLVG 67

Query: 61  CLPTMLT-NKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           CLP ML+ N   TH+WIHS+MKQFN EIASIRLGNT++IP+TSPELA EFLKT
Sbjct: 68  CLPAMLSRNNSSTHEWIHSIMKQFNVEIASIRLGNTYLIPVTSPELALEFLKT 119

[Close]

BLAST of Lsi06G005620 vs. ExPASy TrEMBL
Match: A0A6J1D6G9 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498 PE=3 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.9e-33
Identity = 74/110 (67.27%), Postives = 89/110 (80.91%), Query Frame = 0


Query: 1   MTQLAHQNSPVAISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGC 60
           ++QL +QNSP AI++TF +FVFMAFIS I  Q Y  KRKHSV +   LPPGP PWPLVGC
Sbjct: 7   ISQLTYQNSPTAITKTFTLFVFMAFISAIFSQCYR-KRKHSVLQ---LPPGPNPWPLVGC 66

Query: 61  LPTMLTNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
           LP ML  K P ++WIH+VMKQF+TEIA IRLGNT+++P+TSPELA EFLK
Sbjct: 67  LPAMLATKSPKYKWIHAVMKQFDTEIACIRLGNTYVMPVTSPELAVEFLK 112

[Close]

BLAST of Lsi06G005620 vs. ExPASy TrEMBL
Match: A0A5D3DVF9 (Isoleucine N-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold225G00730 PE=3 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.1e-32
Identity = 73/114 (64.04%), Postives = 89/114 (78.07%), Query Frame = 0


Query: 3   QLAHQNSPVA----ISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLV 62
           QL +QNSPVA    +  +F++F+FMAF+S++ FQ YN   KHS   Q PLPPGPKPWPL+
Sbjct: 10  QLIYQNSPVAMISNLQPSFVMFLFMAFLSILFFQCYNRTEKHS-NLQPPLPPGPKPWPLL 69

Query: 63  GCLPTML-TNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           GCLP ML +N   TH+WIHS+MKQFN EIASIRL NT++IP+ SPELA EFLKT
Sbjct: 70  GCLPAMLSSNNSSTHEWIHSIMKQFNAEIASIRLRNTYVIPVASPELALEFLKT 122

[Close]

BLAST of Lsi06G005620 vs. NCBI nr
Match: XP_038878794.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 182.6 bits (462), Expect = 1.9e-42
Identity = 88/110 (80.00%), Postives = 99/110 (90.00%), Query Frame = 0


Query: 1   MTQLAHQNSPVAISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGC 60
           ++QLA+QNS +AIS+TFIVFVFMAFIS+I    YNLK K SV+R+APLPPGPKPWPLVGC
Sbjct: 8   ISQLAYQNSLIAISKTFIVFVFMAFISVIFSHCYNLKTKRSVRRKAPLPPGPKPWPLVGC 67

Query: 61  LPTMLTNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
           LPTMLT K P HQWIHSVMKQFNTEIASIRLGNT+IIP+TSPELA +FLK
Sbjct: 68  LPTMLTIKYPPHQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLK 117

[Close]

BLAST of Lsi06G005620 vs. NCBI nr
Match: XP_038880015.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 180.3 bits (456), Expect = 9.4e-42
Identity = 87/111 (78.38%), Postives = 101/111 (90.99%), Query Frame = 0


Query: 1   MTQLAHQNSPVAISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGC 60
           ++QLAHQ+SP+AI++TFIVFVFMAF S++    YN +RK SVQ QAPLPPGPKPWPLVGC
Sbjct: 8   ISQLAHQHSPIAITKTFIVFVFMAFTSVLFSHFYNRRRKLSVQ-QAPLPPGPKPWPLVGC 67

Query: 61  LPTMLTNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           LPT+LTNK PT+QWIHSVMKQFNTEIASIRLGNT+IIP+TSPELA +FLKT
Sbjct: 68  LPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKT 117

[Close]

BLAST of Lsi06G005620 vs. NCBI nr
Match: XP_008450367.2 (PREDICTED: isoleucine N-monooxygenase 1-like [Cucumis melo])

HSP 1 Score: 174.1 bits (440), Expect = 6.7e-40
Identity = 82/111 (73.87%), Postives = 99/111 (89.19%), Query Frame = 0


Query: 1   MTQLAHQNSPVAISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGC 60
           +TQL HQNSP+AIS+ FIVF+FMAF S++  + YN ++K+SV R+A LPPGPKPWP+VGC
Sbjct: 9   ITQLPHQNSPIAISKAFIVFLFMAFASVMFSRCYNRRKKYSV-RKALLPPGPKPWPIVGC 68

Query: 61  LPTMLTNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           LPTMLTNK PT+QWIHSVMKQFNTEIA IRLGNT++IP+TSPELA EFL+T
Sbjct: 69  LPTMLTNKSPTYQWIHSVMKQFNTEIACIRLGNTYVIPVTSPELALEFLRT 118

[Close]

BLAST of Lsi06G005620 vs. NCBI nr
Match: XP_004151661.2 (isoleucine N-monooxygenase 1 [Cucumis sativus] >KAE8653524.1 hypothetical protein Csa_007554 [Cucumis sativus])

HSP 1 Score: 172.2 bits (435), Expect = 2.6e-39
Identity = 80/111 (72.07%), Postives = 96/111 (86.49%), Query Frame = 0


Query: 1   MTQLAHQNSPVAISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGC 60
           +TQL HQ+SP+AIS+TFI+F+FMAF S++    YN ++K+SV R+  LPPGPKPWP+VGC
Sbjct: 9   LTQLTHQHSPIAISKTFIIFLFMAFASVMFSHYYNRRKKYSVVRKPRLPPGPKPWPIVGC 68

Query: 61  LPTMLTNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           LPTMLTNK PT  WIHSVMKQFNTEIA IRLG+T+IIP+TSPELA EFLKT
Sbjct: 69  LPTMLTNKSPTSHWIHSVMKQFNTEIACIRLGSTYIIPVTSPELAIEFLKT 119

[Close]

BLAST of Lsi06G005620 vs. NCBI nr
Match: XP_038880438.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 165.2 bits (417), Expect = 3.1e-37
Identity = 84/111 (75.68%), Postives = 94/111 (84.68%), Query Frame = 0


Query: 3   QLAHQNSPVAI-SETFIVFVFMAFISMILFQSY-NLKRKHSVQRQAPLPPGPKPWPLVGC 62
           QLAHQNSP+AI S+T IVF FMA  S++  Q Y N +RKH V R+ PLPPGPKPWPLVGC
Sbjct: 10  QLAHQNSPIAITSKTLIVFGFMALTSILFSQCYCNRRRKHLV-RRPPLPPGPKPWPLVGC 69

Query: 63  LPTMLTNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           LPTML NK PTHQWIHSVMK+ NTEIASIRLGNT++IP+TSPELA EFLKT
Sbjct: 70  LPTMLINKSPTHQWIHSVMKKLNTEIASIRLGNTYVIPVTSPELALEFLKT 119

[Close]

BLAST of Lsi06G005620 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 100.1 bits (248), Expect = 1.2e-21
Identity = 49/95 (51.58%), Postives = 62/95 (65.26%), Query Frame = 0


Query: 23  MAFISMILFQSYNLKRKH-------SVQRQAPLPPGPKPWPLVGCLPTMLTNKIPTHQWI 82
           +AFI  +L  +  +KRK        S  R   LPPGPK WPL+G LP +L    P  +WI
Sbjct: 2   LAFIIGLLLLALTMKRKEKKKTMLISPTRNLSLPPGPKSWPLIGNLPEILGRNKPVFRWI 61

Query: 83  HSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
           HS+MK+ NT+IA IRL NTH+IP+TSP +ARE LK
Sbjct: 62  HSLMKELNTDIACIRLANTHVIPVTSPRIAREILK 96

[Close]

BLAST of Lsi06G005620 vs. TAIR 10
Match: AT5G35917.1 (cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene )

HSP 1 Score: 99.4 bits (246), Expect = 2.0e-21
Identity = 50/96 (52.08%), Postives = 62/96 (64.58%), Query Frame = 0


Query: 23  MAFISMILFQSYNLKRKH--------SVQRQAPLPPGPKPWPLVGCLPTMLTNKIPTHQW 82
           +AFI  +L  S  +KRK         S  R   LPPGPK WPLVG LP +L    P  +W
Sbjct: 9   LAFILGLLLLSLTMKRKETKKTMLMTSHTRNLSLPPGPKSWPLVGNLPEILGRNKPVFRW 68

Query: 83  IHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
           IHS+M++ NT+IA IRL NTH+IP+TSP +ARE LK
Sbjct: 69  IHSLMEELNTDIACIRLANTHVIPVTSPRIAREILK 104

[Close]

BLAST of Lsi06G005620 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 92.4 bits (228), Expect = 2.4e-19
Identity = 41/99 (41.41%), Postives = 65/99 (65.66%), Query Frame = 0


Query: 12  AISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKIPT 71
           + S  +++    AF+++ L            +++  LPPGP  WP++G +PTML ++ P 
Sbjct: 20  SFSTLYLLSTLQAFVAITLVMLLKKLMTDPNKKKPYLPPGPTGWPIIGMIPTMLKSR-PV 79

Query: 72  HQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
            +W+HS+MKQ NTEIA ++LGNTH+I +T P++ARE LK
Sbjct: 80  FRWLHSIMKQLNTEIACVKLGNTHVITVTCPKIAREILK 117

[Close]

BLAST of Lsi06G005620 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 89.0 bits (219), Expect = 2.7e-18
Identity = 41/100 (41.00%), Postives = 64/100 (64.00%), Query Frame = 0


Query: 12  AISETFIVFVFMAFISMILFQSYN-LKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKIP 71
           + +  +++    A  ++      N +K     ++  PLPPGP  +P+VG +P ML N+ P
Sbjct: 21  SFTNMYLLTTLQALAALCFLMILNKIKSSSRNKKLHPLPPGPTGFPIVGMIPAMLKNR-P 80

Query: 72  THQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
             +W+HS+MK+ NTEIA +RLGNTH+IP+T P++ARE  K
Sbjct: 81  VFRWLHSLMKELNTEIACVRLGNTHVIPVTCPKIAREIFK 119

[Close]

BLAST of Lsi06G005620 vs. TAIR 10
Match: AT1G58260.1 (cytochrome p450 79c2 )

HSP 1 Score: 70.9 bits (172), Expect = 7.5e-13
Identity = 33/88 (37.50%), Postives = 53/88 (60.23%), Query Frame = 0


Query: 23  MAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKIPTHQWIHSVMKQF 82
           +  IS+ L  +   +    ++ +  LPPGP+ WP+VG +  M+ N+ P H WIH VM++ 
Sbjct: 12  LVLISITLVLALARRFSRFMKPKGQLPPGPRGWPIVGNMLQMIINR-PAHLWIHRVMEEL 71

Query: 83  NTEIASIRLGNTHIIPITSPELAREFLK 111
            T+IA  R    H+I +TS ++ARE L+
Sbjct: 72  QTDIACYRFARFHVITVTSSKIAREVLR 98

The following BLAST results are available for this feature:

BLAST of Lsi06G005620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q605	4.1e-24	54.46	Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV...	[more]
Q9FLC8	1.6e-20	51.58	Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV...	[more]
O81346	3.4e-18	41.41	Tryptophan N-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP79B2 PE=1 SV=...	[more]
Q6J540	3.4e-18	49.48	Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1	[more]
O81345	2.9e-17	41.75	Cytochrome P450 79B1 OS=Sinapis alba OX=3728 GN=CYP79B1 PE=2 SV=1	[more]

BLAST of Lsi06G005620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BQ37	3.3e-40	73.87	isoleucine N-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103491994 PE=3 S...	[more]
A0A0A0LXR0	1.2e-39	72.07	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G620390 PE=4 SV=1	[more]
A0A0A0LXG7	4.6e-34	68.14	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G618390 PE=4 SV=1	[more]
A0A6J1D6G9	3.9e-33	67.27	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498...	[more]
A0A5D3DVF9	1.1e-32	64.04	Isoleucine N-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

BLAST of Lsi06G005620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878794.1	1.9e-42	80.00	tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida]	[more]
XP_038880015.1	9.4e-42	78.38	tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida]	[more]
XP_008450367.2	6.7e-40	73.87	PREDICTED: isoleucine N-monooxygenase 1-like [Cucumis melo]	[more]
XP_004151661.2	2.6e-39	72.07	isoleucine N-monooxygenase 1 [Cucumis sativus] >KAE8653524.1 hypothetical protei...	[more]
XP_038880438.1	3.1e-37	75.68	tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida]	[more]

BLAST of Lsi06G005620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05260.1	1.2e-21	51.58	cytochrome p450 79a2	[more]
AT5G35917.1	2.0e-21	52.08	cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene	[more]
AT4G39950.1	2.4e-19	41.41	cytochrome P450, family 79, subfamily B, polypeptide 2	[more]
AT2G22330.1	2.7e-18	41.00	cytochrome P450, family 79, subfamily B, polypeptide 3	[more]
AT1G58260.1	7.5e-13	37.50	cytochrome p450 79c2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 36..111 e-value: 7.5E-12 score: 46.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 49..110
None	No IPR available	PANTHER	PTHR24298:SF378	P450 FAMILY PROTEIN, PUTATIVE-RELATED	coord: 16..110
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 16..110

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G005620.1	Lsi06G005620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen