Lsi06G005610 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G005610
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Isoleucine N-monooxygenase 1-like
Location	chr06: 7172847 .. 7175565 (-)
RNA-Seq Expression	Lsi06G005610
Synteny	Lsi06G005610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCCCAGTATTGATGAGAGGGTGCAACAATAGAAGAAATATGAGAAAAAAAGAAGGAATAGAGGACATTCTTGATATCCTTATTTCACTTAAAGATGAGAATGCAAAATCGTTGTTGTCGATCGAAGAGATTAAAGCTCAAATCGCGGTAAGCATTAATTCTACAGCTTTATTTATGATAATAATCACTCATAAATTACTCACATATATATGCAGACATATAAATAATTAAATTATAATTTAATTTAGTCTTCGAACTTTTAGGTTTGTGTTTATATATTTTTGAGCTATTATGTGATCTTTGGACATATTTAACATTTTTAAAATTCATGGACCCTGGTAAACACTAAATTGAAATTTAAAGGACCTCTTAAACATCTTTAAGAGCAATGTTGCACTTATTTCCATGCACCTTCTTCAATTAAAAAGGAAAAAAATATTTTAAAAGTATTTTAAAATTTAAAAAAATATTGCACATATTACTGCTTATAATTTGACAATTGGGTGTAGACTTCACATAGATGGGTGTAGCCAGCCAACTATTGTTTATATTTTTAATATTTTTAATGTTCATGAAAGTTTTAGCCACAAAAATTTAAAGTTGATGAACTTATTAGATTATTAGACACTTTTTAAGATTAAGAGACAAACACGAGCTTAAAAGGAAGCTTCAAAGAACTAAACTAGCCATTTAAGTTCATGAAATTATAGTAATATTATTGTTGTCAATATTTATGTGAACCTTTCCCATCATAATAATTTATATATATTAAAACAATTAAAAATCATTCCTTGAAAAAAACAAATAAAAATAATTGAAGTTATGTTATTAATAAAATCAAGATTAGGTTACCCATTATTCTTCATCAAGGGTACCATTTAAAAAAATAATCGAAGTTTTTGTGTACAGATGAGATAAAGGAAAGTGTGTGATTTGATAACTAAAAATCATTATGTAACTTGGTAATTATTATATAACCATTTACTATAATTTTTGCGTTTTTTATTTTTGAAAATTTAGCTTATAAAACACTACTTTCATCTATAATTTTTTTTTTGTTTAATTAGTTGCTTTCTAAAACCGTGTTTCAAAATTCATACCAAATATTAGAAACAAAAACTAAGGCTTCAATTGATAACTACTTCGGATTTTTGTTTTATTTGTCTATTTATTATTTTGTTTTTATTTATTATTTCTTTTGTTATTTTAAAACTGAAATCATATATATTAGATAGCTAGTTTTATTTACTTTTAAACAAAGGACCAAGTTTGTCTGAGATCTATTTTTTCAATTTTTTAATTAGAGAGATGTGAACAGATGTTTTATAGAGAGAGATATATGTGGAAACAGAAAAAATAAAAAAATTAAAGACTAGGAGTCAGAAACAGTTTGATTTAGTTTGATTTCGTACTCGAACCAAACCGAACTAAATTTTTTTTAACTTTTATATATAAAACCAAACTAACCAATCGGCACGAATCGAATCGAATCAAACCAAATCAGTTCGATTTTAAATTCTGTTTTGTTATTTTTAAAAAATATAATGGTTCGGTTCGGTTCGGTTTGGTTTGGAATTTGGTTTTGCTCTTTTTTTTAAATATATAAATAAATAAATATATATAATAGTTAAAAACAGTTCGGTTCGGTTTTAAATTCAGTTTTCGAAATTGAAACCTAAATGAACCGAATAAATTCGGTTTCAAGATTTCTTCAAACCAGATCGAATTGCATTTTTCAGTTTTACTGAATTTTCAGTTCGATTTTGAAAAACAGTTCGGTTTTTGCGGTTTGAATGACCACCCCTATTAAAGACATATAGAGATAAATGTTTTAAAGAGAAAAATTAATCATGGTAAATTTATATTTAGAGAGAGACAGCTATTAGTTAGACTTTGGAAAAAAATATTAGTTAAAAAATAGAAAGAAGAGTCAGAGGAAAGAGATAAATTTTTTTGGAGAAAAAAGTTGAATAAAAAGTGAGAGTAAAATATATGTTAGAAAAATAAAGATAAATGGAAACAACGAAGTATTTGTAAAGGGAGAGAATTTAGAGAGATTGTCTTTATTGAGAAAAATTAGACATTTTTAAAATTCATAGACAAACACAAGCTTTGAAATGAAAGTTCAAGAATGTCTAAACTGGTAATTTTTGTTTTATGAAATTATAGTTCTATTATTGTTGTCAATACTAACATGAAACTTCCCCACCAAATAATAGGAGTTACAAATTGCAATGATAGATAATCCTTCAAATGCAGTGGAATGGACAATGGTAGAAATGATGAATCAACCAAAAATTTCCAGAAAGTCGTTGAAGAATTGGACGAAGTAGTTGGAAGAAAAAGACTAGTTCAAGAATCTGATATCCCAAAACTCAAGTATCTCACAGCTTGCATTAGAGAATCTTTTCGACTTTATCCATTTTCACCTTTCAATATTCCTCATGTCTCAAACTCGGACATCATTGTGGATGGTTACTTTATTCTCAAAGGTAGTGAAGTCTTACTCAGTCGTCTAGGTCTTGGTCGAAATCCAAGAATTTGGGAGGATCCAATCAGATTTAATCCAGAACGTCACCTAAAAGATGAGACAGTTGAATTGGGACTATCAGAACCAAGTCTTCAATTTATCACCTTCACTAGAGGGAGGAGAGGCTACATTGGTAGCTCTCTGGGCACTAACATTAGCATGATGCTATTTGCTAGACTTCTACAA

mRNA sequence

ATGAGCCCAATGAGAATGCAAAATCGTTGTTGTCGATCGAAGAGATTAAAGCTCAAATCGCGTGGAATGGACAATGGTAGAAATGATGAATCAACCAAAAATTTCCAGAAAGTCGTTGAAGAATTGGACGAAGTAGTTGGAAGAAAAAGACTAGTTCAAGAATCTGATATCCCAAAACTCAAGTATCTCACAGCTTGCATTAGAGAATCTTTTCGACTTTATCCATTTTCACCTTTCAATATTCCTCATGTCTCAAACTCGGACATCATTGTGGATGGTTACTTTATTCTCAAAGGTAGTGAAGTCTTACTCAGTCGTCTAGGTCTTGGTCGAAATCCAAGAATTTGGGAGGATCCAATCAGATTTAATCCAGAACGTCACCTAAAAGATGAGACAGTTGAATTGGGACTATCAGAACCAAGTCTTCAATTTATCACCTTCACTAGAGGGAGGAGAGGCTACATTGGTAGCTCTCTGGGCACTAACATTAGCATGATGCTATTTGCTAGACTTCTACAA

Coding sequence (CDS)

ATGAGCCCAATGAGAATGCAAAATCGTTGTTGTCGATCGAAGAGATTAAAGCTCAAATCGCGTGGAATGGACAATGGTAGAAATGATGAATCAACCAAAAATTTCCAGAAAGTCGTTGAAGAATTGGACGAAGTAGTTGGAAGAAAAAGACTAGTTCAAGAATCTGATATCCCAAAACTCAAGTATCTCACAGCTTGCATTAGAGAATCTTTTCGACTTTATCCATTTTCACCTTTCAATATTCCTCATGTCTCAAACTCGGACATCATTGTGGATGGTTACTTTATTCTCAAAGGTAGTGAAGTCTTACTCAGTCGTCTAGGTCTTGGTCGAAATCCAAGAATTTGGGAGGATCCAATCAGATTTAATCCAGAACGTCACCTAAAAGATGAGACAGTTGAATTGGGACTATCAGAACCAAGTCTTCAATTTATCACCTTCACTAGAGGGAGGAGAGGCTACATTGGTAGCTCTCTGGGCACTAACATTAGCATGATGCTATTTGCTAGACTTCTACAA

Protein sequence

MSPMRMQNRCCRSKRLKLKSRGMDNGRNDESTKNFQKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVDGYFILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRRGYIGSSLGTNISMMLFARLLQ

Homology

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BLAST of Lsi06G005610 vs. ExPASy Swiss-Prot
Match: Q43135 (Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3)

HSP 1 Score: 183.0 bits (463), Expect = 3.0e-45
Identity = 84/137 (61.31%), Postives = 110/137 (80.29%), Query Frame = 0


Query: 37  KVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVDGYFI 96
           K +EELD VVGR+RLVQESDIPKL Y+ ACIRE+FRL+P +PFN+PHV+ +D  + GY +
Sbjct: 376 KAMEELDRVVGRERLVQESDIPKLNYVKACIREAFRLHPVAPFNVPHVALADTTIAGYRV 435

Query: 97  LKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRRGYIG 156
            KGS V+LSR GLGRNPR+W++P+RF P+RHL     ++ L+E  L+FI+F+ GRRG I 
Sbjct: 436 PKGSHVILSRTGLGRNPRVWDEPLRFYPDRHLATAASDVALTENDLRFISFSTGRRGCIA 495

Query: 157 SSLGTNISMMLFARLLQ 174
           +SLGT +S+MLF RLLQ
Sbjct: 496 ASLGTAMSVMLFGRLLQ 512

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BLAST of Lsi06G005610 vs. ExPASy Swiss-Prot
Match: A0A068Q7V0 (Phenylalanine N-monooxygenase CYP79D16 OS=Prunus mume OX=102107 GN=CYP79D16 PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.4e-42
Identity = 78/138 (56.52%), Postives = 111/138 (80.43%), Query Frame = 0


Query: 35  FQKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVDGY 94
           F+K  +ELD VVG++R VQESD+ +L ++ AC RE+FRL+P +PFN+PHVS +D  V  Y
Sbjct: 353 FEKARQELDAVVGKERQVQESDLSQLNFVKACAREAFRLHPVAPFNVPHVSMADTTVGDY 412

Query: 95  FILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRRGY 154
           FI KGS V+LSR+GLGRNP+IW++P+++ PERHLKD+   + L+E  L+FI+F+ G RG 
Sbjct: 413 FIPKGSHVMLSRIGLGRNPKIWDEPLKYKPERHLKDDGSGVVLTESELRFISFSTGMRGC 472

Query: 155 IGSSLGTNISMMLFARLL 173
           + S+LGT++++MLFARLL
Sbjct: 473 VASTLGTSMTVMLFARLL 490

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BLAST of Lsi06G005610 vs. ExPASy Swiss-Prot
Match: Q9FLC8 (Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 3.4e-41
Identity = 76/138 (55.07%), Postives = 106/138 (76.81%), Query Frame = 0


Query: 36  QKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVDGYF 95
           QK VEE+D VVG+ RLV ESD+P L Y+ AC++E+FRL+P +PFN+PH+S +D +VDGYF
Sbjct: 350 QKAVEEIDRVVGKDRLVIESDLPNLNYVKACVKEAFRLHPVAPFNLPHMSTTDTVVDGYF 409

Query: 96  ILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRRGYI 155
           I KGS VL+SR+G+GRNP +W+ P +F+PERHL   T  + L+E  L  I+F+ GRRG +
Sbjct: 410 IPKGSHVLISRMGIGRNPSVWDKPHKFDPERHLSTNTC-VDLNESDLNIISFSAGRRGCM 469

Query: 156 GSSLGTNISMMLFARLLQ 174
           G  +G+ ++ ML ARL+Q
Sbjct: 470 GVDIGSAMTYMLLARLIQ 486

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BLAST of Lsi06G005610 vs. ExPASy Swiss-Prot
Match: O81345 (Cytochrome P450 79B1 OS=Sinapis alba OX=3728 GN=CYP79B1 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 9.9e-41
Identity = 81/138 (58.70%), Postives = 107/138 (77.54%), Query Frame = 0


Query: 36  QKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVDGYF 95
           +K +EE+D VVG++RLVQESDIPKL Y+ A +RE+FRL+P + FN+PHV+ SD  V GY 
Sbjct: 361 RKAMEEIDRVVGKERLVQESDIPKLNYVKAILREAFRLHPVAAFNLPHVALSDATVAGYH 420

Query: 96  ILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRRGYI 155
           I KGS+VLLSR GLGRNP++W DP+ F PERHL +E  E+ L+E  L+FI+F+ G+RG  
Sbjct: 421 IPKGSQVLLSRYGLGRNPKVWADPLSFKPERHL-NECSEVTLTENDLRFISFSTGKRGCA 480

Query: 156 GSSLGTNISMMLFARLLQ 174
             +LGT ++ ML ARLLQ
Sbjct: 481 APALGTALTTMLLARLLQ 497

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BLAST of Lsi06G005610 vs. ExPASy Swiss-Prot
Match: Q501D8 (Tryptophan N-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP79B3 PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.7e-40
Identity = 81/137 (59.12%), Postives = 105/137 (76.64%), Query Frame = 0


Query: 37  KVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVDGYFI 96
           K +EE+D VVG++R VQESDIPKL Y+ A IRE+FRL+P + FN+PHV+ SD  V GY I
Sbjct: 363 KAMEEIDRVVGKERFVQESDIPKLNYVKAIIREAFRLHPVAAFNLPHVALSDTTVAGYHI 422

Query: 97  LKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRRGYIG 156
            KGS+VLLSR GLGRNP++W DP+ F PERHL +E  E+ L+E  L+FI+F+ G+RG   
Sbjct: 423 PKGSQVLLSRYGLGRNPKVWSDPLSFKPERHL-NECSEVTLTENDLRFISFSTGKRGCAA 482

Query: 157 SSLGTNISMMLFARLLQ 174
            +LGT I+ M+ ARLLQ
Sbjct: 483 PALGTAITTMMLARLLQ 498

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BLAST of Lsi06G005610 vs. ExPASy TrEMBL
Match: A0A6J1D6G9 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 4.0e-61
Identity = 117/141 (82.98%), Postives = 130/141 (92.20%), Query Frame = 0


Query: 33  KNFQKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVD 92
           K  QK +EELD VVGR+RLVQESDIPKLKYLTAC RESFRL+PFSPFNIPHVSN+DI+V 
Sbjct: 358 KILQKAIEELDRVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNIPHVSNADIVVA 417

Query: 93  GYFILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRR 152
           GYFI KGSEV+LSR GLGRNPRIWEDP+RF+PERHLKDETVELGLSEP+L+FI+FTRGRR
Sbjct: 418 GYFIPKGSEVILSRSGLGRNPRIWEDPMRFDPERHLKDETVELGLSEPNLRFISFTRGRR 477

Query: 153 GYIGSSLGTNISMMLFARLLQ 174
           G +GSSLGTNI+MML  RLLQ
Sbjct: 478 GCVGSSLGTNITMMLLGRLLQ 498

[Close]

BLAST of Lsi06G005610 vs. ExPASy TrEMBL
Match: A0A5D3C9F7 (Phenylalanine N-monooxygenase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1933G00190 PE=3 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.5e-60
Identity = 117/141 (82.98%), Postives = 130/141 (92.20%), Query Frame = 0


Query: 33  KNFQKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVD 92
           K  QKVVEELDEVVGR RLVQE DIP LKYLTAC+RESFRL+PFSPFN+PHVS SDI+V 
Sbjct: 9   KILQKVVEELDEVVGRDRLVQEFDIPNLKYLTACMRESFRLHPFSPFNLPHVSISDIVVG 68

Query: 93  GYFILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRR 152
           GYFI KGS++LL R+GLGRNPRIWEDP+RF+PERHL+DETV+ GLSEPSL+FITFTRGRR
Sbjct: 69  GYFIPKGSQILLCRVGLGRNPRIWEDPMRFDPERHLRDETVKFGLSEPSLRFITFTRGRR 128

Query: 153 GYIGSSLGTNISMMLFARLLQ 174
           G IGSSLGTNI+MMLFARLLQ
Sbjct: 129 GCIGSSLGTNITMMLFARLLQ 149

[Close]

BLAST of Lsi06G005610 vs. ExPASy TrEMBL
Match: A0A1S3BNA6 (phenylalanine N-monooxygenase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491937 PE=3 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.5e-60
Identity = 117/141 (82.98%), Postives = 130/141 (92.20%), Query Frame = 0


Query: 33  KNFQKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVD 92
           K  QKVVEELDEVVGR RLVQE DIP LKYLTAC+RESFRL+PFSPFN+PHVS SDI+V 
Sbjct: 360 KILQKVVEELDEVVGRDRLVQEFDIPNLKYLTACMRESFRLHPFSPFNLPHVSISDIVVG 419

Query: 93  GYFILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRR 152
           GYFI KGS++LL R+GLGRNPRIWEDP+RF+PERHL+DETV+ GLSEPSL+FITFTRGRR
Sbjct: 420 GYFIPKGSQILLCRVGLGRNPRIWEDPMRFDPERHLRDETVKFGLSEPSLRFITFTRGRR 479

Query: 153 GYIGSSLGTNISMMLFARLLQ 174
           G IGSSLGTNI+MMLFARLLQ
Sbjct: 480 GCIGSSLGTNITMMLFARLLQ 500

[Close]

BLAST of Lsi06G005610 vs. ExPASy TrEMBL
Match: A0A1S3BPW4 (phenylalanine N-monooxygenase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491937 PE=3 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.5e-60
Identity = 117/141 (82.98%), Postives = 130/141 (92.20%), Query Frame = 0


Query: 33  KNFQKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVD 92
           K  QKVVEELDEVVGR RLVQE DIP LKYLTAC+RESFRL+PFSPFN+PHVS SDI+V 
Sbjct: 360 KILQKVVEELDEVVGRDRLVQEFDIPNLKYLTACMRESFRLHPFSPFNLPHVSISDIVVG 419

Query: 93  GYFILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRR 152
           GYFI KGS++LL R+GLGRNPRIWEDP+RF+PERHL+DETV+ GLSEPSL+FITFTRGRR
Sbjct: 420 GYFIPKGSQILLCRVGLGRNPRIWEDPMRFDPERHLRDETVKFGLSEPSLRFITFTRGRR 479

Query: 153 GYIGSSLGTNISMMLFARLLQ 174
           G IGSSLGTNI+MMLFARLLQ
Sbjct: 480 GCIGSSLGTNITMMLFARLLQ 500

[Close]

BLAST of Lsi06G005610 vs. ExPASy TrEMBL
Match: A0A6J1D470 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017448 PE=3 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 2.0e-60
Identity = 115/142 (80.99%), Postives = 132/142 (92.96%), Query Frame = 0


Query: 32  TKNFQKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIV 91
           +K  QKV+EE+D +VGR RLVQESDIPKLKYLTAC+RESFRL+PFSPFN+PHVSNSDIIV
Sbjct: 344 SKILQKVIEEMDRIVGRDRLVQESDIPKLKYLTACVRESFRLHPFSPFNVPHVSNSDIIV 403

Query: 92  DGYFILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGR 151
            GYFI KGSEV+LSRLGLGRN RIW+DP+RF+PERHLKD TVE GLSEPSL+FI+F+RGR
Sbjct: 404 AGYFIPKGSEVVLSRLGLGRNTRIWKDPMRFDPERHLKDGTVEFGLSEPSLRFISFSRGR 463

Query: 152 RGYIGSSLGTNISMMLFARLLQ 174
           RG +GSSLGT+I+MMLFARLLQ
Sbjct: 464 RGCVGSSLGTSITMMLFARLLQ 485

[Close]

BLAST of Lsi06G005610 vs. NCBI nr
Match: XP_038878794.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 249.6 bits (636), Expect = 2.0e-62
Identity = 121/139 (87.05%), Postives = 134/139 (96.40%), Query Frame = 0


Query: 36  QKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVDGYF 95
           +K VEELDEVVGR+RLV+ESDIPKLKYLTACIRESFRL+PFSPFNIPHVSNSDI+V+GY 
Sbjct: 363 KKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIVVNGYL 422

Query: 96  ILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETV-ELGLSEPSLQFITFTRGRRGY 155
           I KGSEVLLSR+GLGRNPRIWEDP+RFNPERHLKD+TV ELG+SEPSL+FITFTRGRRG 
Sbjct: 423 IPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTVTELGISEPSLRFITFTRGRRGC 482

Query: 156 IGSSLGTNISMMLFARLLQ 174
           +GSSLGTNI+MMLFARLLQ
Sbjct: 483 VGSSLGTNITMMLFARLLQ 501

[Close]

BLAST of Lsi06G005610 vs. NCBI nr
Match: XP_038880015.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 248.8 bits (634), Expect = 3.4e-62
Identity = 121/139 (87.05%), Postives = 134/139 (96.40%), Query Frame = 0


Query: 36  QKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVDGYF 95
           +K VEELDEVVGR+RLV+ESDIPKLKYLTACIRESFRL+PFSPFNIPHVSNSDIIV+GY 
Sbjct: 362 KKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVNGYL 421

Query: 96  ILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETV-ELGLSEPSLQFITFTRGRRGY 155
           I KGSEVLLSR+GLGRNPRIWEDP+RFNPERHLKD+T+ ELG+SEPSL+FITFTRGRRG 
Sbjct: 422 IPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGRRGC 481

Query: 156 IGSSLGTNISMMLFARLLQ 174
           +GSSLGTNI+MMLFARLLQ
Sbjct: 482 VGSSLGTNITMMLFARLLQ 500

[Close]

BLAST of Lsi06G005610 vs. NCBI nr
Match: XP_022148957.1 (isoleucine N-monooxygenase 2-like [Momordica charantia])

HSP 1 Score: 244.2 bits (622), Expect = 8.3e-61
Identity = 117/141 (82.98%), Postives = 130/141 (92.20%), Query Frame = 0


Query: 33  KNFQKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVD 92
           K  QK +EELD VVGR+RLVQESDIPKLKYLTAC RESFRL+PFSPFNIPHVSN+DI+V 
Sbjct: 358 KILQKAIEELDRVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNIPHVSNADIVVA 417

Query: 93  GYFILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRR 152
           GYFI KGSEV+LSR GLGRNPRIWEDP+RF+PERHLKDETVELGLSEP+L+FI+FTRGRR
Sbjct: 418 GYFIPKGSEVILSRSGLGRNPRIWEDPMRFDPERHLKDETVELGLSEPNLRFISFTRGRR 477

Query: 153 GYIGSSLGTNISMMLFARLLQ 174
           G +GSSLGTNI+MML  RLLQ
Sbjct: 478 GCVGSSLGTNITMMLLGRLLQ 498

[Close]

BLAST of Lsi06G005610 vs. NCBI nr
Match: TYK07874.1 (phenylalanine N-monooxygenase-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 242.3 bits (617), Expect = 3.1e-60
Identity = 117/141 (82.98%), Postives = 130/141 (92.20%), Query Frame = 0


Query: 33  KNFQKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVD 92
           K  QKVVEELDEVVGR RLVQE DIP LKYLTAC+RESFRL+PFSPFN+PHVS SDI+V 
Sbjct: 9   KILQKVVEELDEVVGRDRLVQEFDIPNLKYLTACMRESFRLHPFSPFNLPHVSISDIVVG 68

Query: 93  GYFILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRR 152
           GYFI KGS++LL R+GLGRNPRIWEDP+RF+PERHL+DETV+ GLSEPSL+FITFTRGRR
Sbjct: 69  GYFIPKGSQILLCRVGLGRNPRIWEDPMRFDPERHLRDETVKFGLSEPSLRFITFTRGRR 128

Query: 153 GYIGSSLGTNISMMLFARLLQ 174
           G IGSSLGTNI+MMLFARLLQ
Sbjct: 129 GCIGSSLGTNITMMLFARLLQ 149

[Close]

BLAST of Lsi06G005610 vs. NCBI nr
Match: XP_008450287.1 (PREDICTED: phenylalanine N-monooxygenase-like isoform X1 [Cucumis melo])

HSP 1 Score: 242.3 bits (617), Expect = 3.1e-60
Identity = 117/141 (82.98%), Postives = 130/141 (92.20%), Query Frame = 0


Query: 33  KNFQKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVD 92
           K  QKVVEELDEVVGR RLVQE DIP LKYLTAC+RESFRL+PFSPFN+PHVS SDI+V 
Sbjct: 360 KILQKVVEELDEVVGRDRLVQEFDIPNLKYLTACMRESFRLHPFSPFNLPHVSISDIVVG 419

Query: 93  GYFILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRR 152
           GYFI KGS++LL R+GLGRNPRIWEDP+RF+PERHL+DETV+ GLSEPSL+FITFTRGRR
Sbjct: 420 GYFIPKGSQILLCRVGLGRNPRIWEDPMRFDPERHLRDETVKFGLSEPSLRFITFTRGRR 479

Query: 153 GYIGSSLGTNISMMLFARLLQ 174
           G IGSSLGTNI+MMLFARLLQ
Sbjct: 480 GCIGSSLGTNITMMLFARLLQ 500

[Close]

BLAST of Lsi06G005610 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 169.5 bits (428), Expect = 2.4e-42
Identity = 76/138 (55.07%), Postives = 106/138 (76.81%), Query Frame = 0


Query: 36  QKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVDGYF 95
           QK VEE+D VVG+ RLV ESD+P L Y+ AC++E+FRL+P +PFN+PH+S +D +VDGYF
Sbjct: 344 QKAVEEIDRVVGKDRLVIESDLPNLNYVKACVKEAFRLHPVAPFNLPHMSTTDTVVDGYF 403

Query: 96  ILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRRGYI 155
           I KGS VL+SR+G+GRNP +W+ P +F+PERHL   T  + L+E  L  I+F+ GRRG +
Sbjct: 404 IPKGSHVLISRMGIGRNPSVWDKPHKFDPERHLSTNTC-VDLNESDLNIISFSAGRRGCM 463

Query: 156 GSSLGTNISMMLFARLLQ 174
           G  +G+ ++ ML ARL+Q
Sbjct: 464 GVDIGSAMTYMLLARLIQ 480

[Close]

BLAST of Lsi06G005610 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 167.2 bits (422), Expect = 1.2e-41
Identity = 81/137 (59.12%), Postives = 105/137 (76.64%), Query Frame = 0


Query: 37  KVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVDGYFI 96
           K +EE+D VVG++R VQESDIPKL Y+ A IRE+FRL+P + FN+PHV+ SD  V GY I
Sbjct: 363 KAMEEIDRVVGKERFVQESDIPKLNYVKAIIREAFRLHPVAAFNLPHVALSDTTVAGYHI 422

Query: 97  LKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRRGYIG 156
            KGS+VLLSR GLGRNP++W DP+ F PERHL +E  E+ L+E  L+FI+F+ G+RG   
Sbjct: 423 PKGSQVLLSRYGLGRNPKVWSDPLSFKPERHL-NECSEVTLTENDLRFISFSTGKRGCAA 482

Query: 157 SSLGTNISMMLFARLLQ 174
            +LGT I+ M+ ARLLQ
Sbjct: 483 PALGTAITTMMLARLLQ 498

[Close]

BLAST of Lsi06G005610 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 166.4 bits (420), Expect = 2.0e-41
Identity = 80/138 (57.97%), Postives = 107/138 (77.54%), Query Frame = 0


Query: 36  QKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVDGYF 95
           +K +EE+D VVG++RLVQESDIPKL Y+ A +RE+FRL+P + FN+PHV+ SD  V GY 
Sbjct: 360 RKAMEEIDRVVGKERLVQESDIPKLNYVKAILREAFRLHPVAAFNLPHVALSDTTVAGYH 419

Query: 96  ILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRRGYI 155
           I KGS+VLLSR GLGRNP++W DP+ F PERHL +E  E+ L+E  L+FI+F+ G+RG  
Sbjct: 420 IPKGSQVLLSRYGLGRNPKVWADPLCFKPERHL-NECSEVTLTENDLRFISFSTGKRGCA 479

Query: 156 GSSLGTNISMMLFARLLQ 174
             +LGT ++ M+ ARLLQ
Sbjct: 480 APALGTALTTMMLARLLQ 496

[Close]

BLAST of Lsi06G005610 vs. TAIR 10
Match: AT1G79370.1 (cytochrome P450, family 79, subfamily C, polypeptide 1 )

HSP 1 Score: 155.6 bits (392), Expect = 3.6e-38
Identity = 70/138 (50.72%), Postives = 102/138 (73.91%), Query Frame = 0


Query: 36  QKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVDGYF 95
           +K VEE+D VVG++RLVQESD+P L Y+ AC RE+ RL+P +PF +PH++  D  + GYF
Sbjct: 361 EKAVEEIDMVVGKERLVQESDVPNLNYVKACCRETLRLHPTNPFLVPHMARHDTTLAGYF 420

Query: 96  ILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDETVELGLSEPSLQFITFTRGRRGYI 155
           I KGS +L+SR G+GRNP+ W++P+ + PERH+     E+ L+EP L+ ++F  GRRG +
Sbjct: 421 IPKGSHILVSRPGVGRNPKTWDEPLIYRPERHITGN--EVVLTEPDLRLVSFGTGRRGCV 480

Query: 156 GSSLGTNISMMLFARLLQ 174
           G+ LGT++ + L  RLLQ
Sbjct: 481 GAKLGTSMIVTLLGRLLQ 496

[Close]

BLAST of Lsi06G005610 vs. TAIR 10
Match: AT1G16410.1 (cytochrome p450 79f1 )

HSP 1 Score: 151.8 bits (382), Expect = 5.2e-37
Identity = 73/140 (52.14%), Postives = 101/140 (72.14%), Query Frame = 0


Query: 36  QKVVEELDEVVGRKRLVQESDIPKLKYLTACIRESFRLYPFSPFNIPHVSNSDIIVDGYF 95
           +K ++ELDEVVGR RLVQESDIP L YL AC RE+FR++P + +   H++  D  + GYF
Sbjct: 353 RKALKELDEVVGRDRLVQESDIPNLNYLKACCRETFRIHPSAHYVPSHLARQDTTLGGYF 412

Query: 96  ILKGSEVLLSRLGLGRNPRIWEDPIRFNPERHLKDE--TVELGLSEPSLQFITFTRGRRG 155
           I KGS + + R GLGRNP+IW+DP+ + PERHL+ +  T E+ L E  ++F++F+ GRRG
Sbjct: 413 IPKGSHIHVCRPGLGRNPKIWKDPLVYKPERHLQGDGITKEVTLVETEMRFVSFSTGRRG 472

Query: 156 YIGSSLGTNISMMLFARLLQ 174
            IG  +GT + +ML AR LQ
Sbjct: 473 CIGVKVGTIMMVMLLARFLQ 492

The following BLAST results are available for this feature:

BLAST of Lsi06G005610 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43135	3.0e-45	61.31	Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3	[more]
A0A068Q7V0	1.4e-42	56.52	Phenylalanine N-monooxygenase CYP79D16 OS=Prunus mume OX=102107 GN=CYP79D16 PE=1...	[more]
Q9FLC8	3.4e-41	55.07	Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV...	[more]
O81345	9.9e-41	58.70	Cytochrome P450 79B1 OS=Sinapis alba OX=3728 GN=CYP79B1 PE=2 SV=1	[more]
Q501D8	1.7e-40	59.12	Tryptophan N-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP79B3 PE=1 SV=...	[more]

BLAST of Lsi06G005610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D6G9	4.0e-61	82.98	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498...	[more]
A0A5D3C9F7	1.5e-60	82.98	Phenylalanine N-monooxygenase-like isoform X1 OS=Cucumis melo var. makuwa OX=119...	[more]
A0A1S3BNA6	1.5e-60	82.98	phenylalanine N-monooxygenase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A1S3BPW4	1.5e-60	82.98	phenylalanine N-monooxygenase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1D470	2.0e-60	80.99	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017448...	[more]

BLAST of Lsi06G005610 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878794.1	2.0e-62	87.05	tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida]	[more]
XP_038880015.1	3.4e-62	87.05	tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida]	[more]
XP_022148957.1	8.3e-61	82.98	isoleucine N-monooxygenase 2-like [Momordica charantia]	[more]
TYK07874.1	3.1e-60	82.98	phenylalanine N-monooxygenase-like isoform X1 [Cucumis melo var. makuwa]	[more]
XP_008450287.1	3.1e-60	82.98	PREDICTED: phenylalanine N-monooxygenase-like isoform X1 [Cucumis melo]	[more]

BLAST of Lsi06G005610 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05260.1	2.4e-42	55.07	cytochrome p450 79a2	[more]
AT2G22330.1	1.2e-41	59.12	cytochrome P450, family 79, subfamily B, polypeptide 3	[more]
AT4G39950.1	2.0e-41	57.97	cytochrome P450, family 79, subfamily B, polypeptide 2	[more]
AT1G79370.1	3.6e-38	50.72	cytochrome P450, family 79, subfamily C, polypeptide 1	[more]
AT1G16410.1	5.2e-37	52.14	cytochrome p450 79f1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 144..154 score: 35.65 coord: 154..173 score: 21.59 coord: 105..129 score: 30.61 coord: 64..82 score: 37.54
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 33..173 e-value: 4.9E-40 score: 139.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 35..173
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 36..173 e-value: 2.1E-35 score: 122.4
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 33..173
None	No IPR available	PANTHER	PTHR24298:SF675	PHENYLALANINE N-MONOOXYGENASE	coord: 33..173

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G005610.1	Lsi06G005610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen