Lsi06G005580 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G005580
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Isoleucine N-monooxygenase 1
Location	chr06: 7133351 .. 7134364 (+)
RNA-Seq Expression	Lsi06G005580
Synteny	Lsi06G005580

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGCTTTGTTATTGATCAGTCCATCATCATATCAAAACTCTGCAAAAGCAGCCATAGCTATAACCTTCATTGCTATCATTTTATTCATATCATCCATTATTTATGTTATTAGATTTTCTCAATATTATCGCAAAAGAACTCCCTCATTCCCGCTGCTTCCACCTGGCCCAAAGCCATGGCCTCTCCTTGGTTGCCTTCCAGCAATGTTGAGCAACAAAGACTTGCCAACACACCAATGGATCCATTCAATTATGAAGGAATTCAACACTGAAATTGCTTGCATCCGTCTCGGATTAAATACTCACATAATTCCTGTAACTTCTCCAGAACTTGCTCTTGAATTTCTAAACACACATGATTCTGTCTTTGGATCACGACCCATTTCCATGACTGCGGAGATATTGAGTCGCGGATATCTCAGTACAGTTCTTTCACCCATGGGGGATCAATGGAAGAAAATGAGAAGGATACTTGTTTCTCAAGTACTCAATCCTTCAACCCTCCATCGGATGCTTGGCCAAAGAACAAAGGAAGCTGATACTCTTCTTCGCTACATTTTCATCCTTACAAGAAATGGAGAAGCCGTTGACATAAGAAGCATTACACAACATTATTGTGGTAACATTATTAGAAGAATGATGTTTAATAGAAGATACTATGGAAAAGGGAGGGAAGATGGAGGACCAACTTTTGAAGAAGAAGGCCATAATCAAGCATTGCTTACCATTCTTAAGCATGTTAATGCATTTTCTATATCAGATTTCATACCTTTTTTGAAGCCATTGGATTTGGATGGACATGGAAAGATTCTAGAAAAGGCTTTGAATTTCATAAGAAGGTATGATGATCCTATTATAGACGAAAGATTGCAGCAATGGAAAGATGGGAAGAAGAAAGAGGTCGAGGAAGACGACGTTCTTGATATCCTCCTCTCACTTAAAGATGAGAATGGAAAATCATTGTTGTCAATCGATGAGATTAAGGCCCAAATCATGGTAAGCATTTTCTAG

mRNA sequence

ATGAAAGCTTTGTTATTGATCAGTCCATCATCATATCAAAACTCTGCAAAAGCAGCCATAGCTATAACCTTCATTGCTATCATTTTATTCATATCATCCATTATTTATGTTATTAGATTTTCTCAATATTATCGCAAAAGAACTCCCTCATTCCCGCTGCTTCCACCTGGCCCAAAGCCATGGCCTCTCCTTGGTTGCCTTCCAGCAATGTTGAGCAACAAAGACTTGCCAACACACCAATGGATCCATTCAATTATGAAGGAATTCAACACTGAAATTGCTTGCATCCGTCTCGGATTAAATACTCACATAATTCCTGTAACTTCTCCAGAACTTGCTCTTGAATTTCTAAACACACATGATTCTGTCTTTGGATCACGACCCATTTCCATGACTGCGGAGATATTGAGTCGCGGATATCTCAGTACAGTTCTTTCACCCATGGGGGATCAATGGAAGAAAATGAGAAGGATACTTGTTTCTCAAGTACTCAATCCTTCAACCCTCCATCGGATGCTTGGCCAAAGAACAAAGGAAGCTGATACTCTTCTTCGCTACATTTTCATCCTTACAAGAAATGGAGAAGCCGTTGACATAAGAAGCATTACACAACATTATTGTGGTAACATTATTAGAAGAATGATGTTTAATAGAAGATACTATGGAAAAGGGAGGGAAGATGGAGGACCAACTTTTGAAGAAGAAGGCCATAATCAAGCATTGCTTACCATTCTTAAGCATGTTAATGCATTTTCTATATCAGATTTCATACCTTTTTTGAAGCCATTGGATTTGGATGGACATGGAAAGATTCTAGAAAAGGCTTTGAATTTCATAAGAAGGTATGATGATCCTATTATAGACGAAAGATTGCAGCAATGGAAAGATGGGAAGAAGAAAGAGGTCGAGGAAGACGACGTTCTTGATATCCTCCTCTCACTTAAAGATGAGAATGGAAAATCATTGTTGTCAATCGATGAGATTAAGGCCCAAATCATGGTAAGCATTTTCTAG

Coding sequence (CDS)

ATGAAAGCTTTGTTATTGATCAGTCCATCATCATATCAAAACTCTGCAAAAGCAGCCATAGCTATAACCTTCATTGCTATCATTTTATTCATATCATCCATTATTTATGTTATTAGATTTTCTCAATATTATCGCAAAAGAACTCCCTCATTCCCGCTGCTTCCACCTGGCCCAAAGCCATGGCCTCTCCTTGGTTGCCTTCCAGCAATGTTGAGCAACAAAGACTTGCCAACACACCAATGGATCCATTCAATTATGAAGGAATTCAACACTGAAATTGCTTGCATCCGTCTCGGATTAAATACTCACATAATTCCTGTAACTTCTCCAGAACTTGCTCTTGAATTTCTAAACACACATGATTCTGTCTTTGGATCACGACCCATTTCCATGACTGCGGAGATATTGAGTCGCGGATATCTCAGTACAGTTCTTTCACCCATGGGGGATCAATGGAAGAAAATGAGAAGGATACTTGTTTCTCAAGTACTCAATCCTTCAACCCTCCATCGGATGCTTGGCCAAAGAACAAAGGAAGCTGATACTCTTCTTCGCTACATTTTCATCCTTACAAGAAATGGAGAAGCCGTTGACATAAGAAGCATTACACAACATTATTGTGGTAACATTATTAGAAGAATGATGTTTAATAGAAGATACTATGGAAAAGGGAGGGAAGATGGAGGACCAACTTTTGAAGAAGAAGGCCATAATCAAGCATTGCTTACCATTCTTAAGCATGTTAATGCATTTTCTATATCAGATTTCATACCTTTTTTGAAGCCATTGGATTTGGATGGACATGGAAAGATTCTAGAAAAGGCTTTGAATTTCATAAGAAGGTATGATGATCCTATTATAGACGAAAGATTGCAGCAATGGAAAGATGGGAAGAAGAAAGAGGTCGAGGAAGACGACGTTCTTGATATCCTCCTCTCACTTAAAGATGAGAATGGAAAATCATTGTTGTCAATCGATGAGATTAAGGCCCAAATCATGGTAAGCATTTTCTAG

Protein sequence

MKALLLISPSSYQNSAKAAIAITFIAIILFISSIIYVIRFSQYYRKRTPSFPLLPPGPKPWPLLGCLPAMLSNKDLPTHQWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFLNTHDSVFGSRPISMTAEILSRGYLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRTKEADTLLRYIFILTRNGEAVDIRSITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGHNQALLTILKHVNAFSISDFIPFLKPLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDGKKKEVEEDDVLDILLSLKDENGKSLLSIDEIKAQIMVSIF

Homology

[Close]

BLAST of Lsi06G005580 vs. ExPASy Swiss-Prot
Match: A0A068Q605 (Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 4.1e-91
Identity = 159/310 (51.29%), Postives = 231/310 (74.52%), Query Frame = 0


Query: 25  IAIILFISSIIYVIRFSQYYRKRTPSFPLLPPGPKPWPLLGCLPAMLSNKDLPTHQWIHS 84
           +A I+F+  + ++++   + +   P  P LPPGP PWP++G LP M  N+  P H+WIHS
Sbjct: 20  LAFIIFMFLVKFILK--THNKNSVPVVP-LPPGPSPWPIVGSLPEMWRNR--PAHRWIHS 79

Query: 85  IMKEFNTEIACIRLGLNTHIIPVTSPELALEFLNTHDSVFGSRPISMTAEILSRGYLSTV 144
           +MK+ NT+IACIRLG N H+IPVTSPE+A EFL  +D+VF SRP++M  + LS GYL+TV
Sbjct: 80  LMKKLNTDIACIRLG-NVHVIPVTSPEIAREFLKKNDAVFASRPVTMATKTLSSGYLTTV 139

Query: 145 LSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRTKEADTLLRYIF---ILTRNGEAVDIRS 204
           + P GDQW+KMRR+LV++  NPS +H +LG+R +EAD L+++++      +NG  V++R 
Sbjct: 140 VGPWGDQWRKMRRVLVAEAFNPSRVHWLLGKRNEEADNLVKFLYNQCSANQNGAVVNVRI 199

Query: 205 ITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGHNQALLTILKHVNAFSISDFIPFLK 264
             Q Y G+I+R+M+FNR Y+GKGREDGGP  EEE H  ALLT+L +  AF +SD++P+L+
Sbjct: 200 AAQFYSGSIMRKMIFNRTYFGKGREDGGPGVEEEEHVSALLTLLTYAYAFCVSDYLPWLR 259

Query: 265 PLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDGKKKEVEEDDVLDILLSLKDENGKS 324
             D+DGH K + KA+N ++++ +PI++ERLQ+W+DGK+   E DD+LD+ +SLKD NG+ 
Sbjct: 260 VFDIDGHEKKVRKAMNIVKKHQEPIVNERLQEWRDGKRN--EPDDLLDVFISLKDANGQP 319

Query: 325 LLSIDEIKAQ 332
           LLS +EIKAQ
Sbjct: 320 LLSDEEIKAQ 321

[Close]

BLAST of Lsi06G005580 vs. ExPASy Swiss-Prot
Match: Q6J541 (Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.0e-82
Identity = 159/316 (50.32%), Postives = 215/316 (68.04%), Query Frame = 0


Query: 23  TFIAIILFISSIIYVIRFSQYYRKRTPSFPLLPPGPKPWPLLGCLPAMLSNKDLPTHQWI 82
           TF+ +++F   I  V +     + +      LPPGPKPWP++G LP ML+N+  P   WI
Sbjct: 17  TFLLVVIFSFMIFKVTKTHLVNKSKKYK---LPPGPKPWPIVGNLPEMLANR--PATIWI 76

Query: 83  HSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFLNTHDSVFGSRPISMTAEILSRGYLS 142
           H +MKE NTEIACIRL  NT +IPVT P +A EFL  HD+ F SRP  M+ +I S G+++
Sbjct: 77  HKLMKEMNTEIACIRLA-NTIVIPVTCPTIACEFLKKHDASFASRPKIMSTDIASDGFIT 136

Query: 143 TVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRTKEADTLLRYIF-----ILTRNGEAV 202
           TVL P G+QWKKM+R+LV+ +L+P     +LG+R +EAD L+ YI+      +      V
Sbjct: 137 TVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKDVNDGPGLV 196

Query: 203 DIRSITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGHNQALLTILKHVNAFSISDFI 262
           +IR   QHY GN+ R+++FN RY+GK  EDGGP FEE  H  A  TILK+V AFSISDF+
Sbjct: 197 NIRIAAQHYGGNVFRKLIFNSRYFGKVMEDGGPGFEEVEHINATFTILKYVYAFSISDFV 256

Query: 263 PFLKPLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDGKKKEVEEDDVLDILLSLKDE 322
           PFL+ LDLDGH   + KA+  +R+Y DPIID+R++QW DG K    E+D+LD+L+ LKD 
Sbjct: 257 PFLRRLDLDGHRSKIMKAMRIMRKYHDPIIDDRIKQWNDGLK--TVEEDLLDVLIKLKDA 316

Query: 323 NGKSLLSIDEIKAQIM 334
           N K LL++ E+KAQI+
Sbjct: 317 NNKPLLTLKELKAQII 324

[Close]

BLAST of Lsi06G005580 vs. ExPASy Swiss-Prot
Match: Q6J540 (Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 1.7e-81
Identity = 159/315 (50.48%), Postives = 213/315 (67.62%), Query Frame = 0


Query: 23  TFIAIILFISSIIYVIRFSQYYRKRTPSFPLLPPGPKPWPLLGCLPAMLSNKDLPTHQWI 82
           TF+ +++F   I  V +     + +      LPPGPKPWP++G LP ML+N+  P   WI
Sbjct: 17  TFLLVVIFFFIIFKVTKSHSVNKSKKYK---LPPGPKPWPIVGNLPEMLANR--PATIWI 76

Query: 83  HSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFLNTHDSVFGSRPISMTAEILSRGYLS 142
           H +MKE NTEIACIRL  NT +IPVT P +A EFL  HD+ F SRP  M+ +I S G+L+
Sbjct: 77  HKLMKEMNTEIACIRLA-NTIVIPVTCPTIACEFLKKHDASFASRPKIMSTDIASDGFLT 136

Query: 143 TVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRTKEADTLLRYIF-----ILTRNGEAV 202
           TVL P G+QWKKM+R+LV+ +L+P     +LG+R +EAD L+ YI+      +      V
Sbjct: 137 TVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKDVNDGPGLV 196

Query: 203 DIRSITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGHNQALLTILKHVNAFSISDFI 262
           +IR   QHY GN+ R+++FN RY+GK  EDGGP FEE  H  A  TILK+V AFSISDFI
Sbjct: 197 NIRIAAQHYGGNVFRKLIFNTRYFGKVMEDGGPGFEEVEHINATFTILKYVYAFSISDFI 256

Query: 263 PFLKPLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDGKKKEVEEDDVLDILLSLKDE 322
           PFL+ LDLDGH   + KA+  +++Y DPII +R++QW DG K    E+D+LD+L+ LKD 
Sbjct: 257 PFLRRLDLDGHRSKIMKAMGIMKKYHDPIIHDRIKQWNDGLK--TVEEDLLDVLIKLKDA 316

Query: 323 NGKSLLSIDEIKAQI 333
           + K LL++ EIKAQI
Sbjct: 317 SNKPLLTLKEIKAQI 323

[Close]

BLAST of Lsi06G005580 vs. ExPASy Swiss-Prot
Match: Q9FLC8 (Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.3e-76
Identity = 143/285 (50.18%), Postives = 199/285 (69.82%), Query Frame = 0


Query: 54  LPPGPKPWPLLGCLPAMLSNKDLPTHQWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELA 113
           LPPGPK WPL+G LP +L  ++ P  +WIHS+MKE NT+IACIRL  NTH+IPVTSP +A
Sbjct: 40  LPPGPKSWPLIGNLPEIL-GRNKPVFRWIHSLMKELNTDIACIRLA-NTHVIPVTSPRIA 99

Query: 114 LEFLNTHDSVFGSRPISMTAEILSRGYLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRML 173
            E L   DSVF +RP++M  E  SRGYL+  + P G+QWKKMRR++ S V +  +   ML
Sbjct: 100 REILKKQDSVFATRPLTMGTEYCSRGYLTVAVEPQGEQWKKMRRVVASHVTSKKSFQMML 159

Query: 174 GQRTKEADTLLRYI--FILTRNGEA---VDIRSITQHYCGNIIRRMMFNRRYYGKGREDG 233
            +RT+EAD L+RYI    +   G A   +D+R   + Y GN+ R+MMF  R++GKG EDG
Sbjct: 160 QKRTEEADNLVRYINNRSVKNRGNAFVVIDLRLAVRQYSGNVARKMMFGIRHFGKGSEDG 219

Query: 234 -GPTFEEEGHNQALLTILKHVNAFSISDFIPFLKPLDLDGHGKILEKALNFIRRYDDPII 293
            GP  EE  H ++L T+L H+ AF++SD++P+L+ LDL+GH K++  A+  + +Y+DP +
Sbjct: 220 SGPGLEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVSNAMRNVSKYNDPFV 279

Query: 294 DERLQQWKDGKKKEVEEDDVLDILLSLKDENGKSLLSIDEIKAQI 333
           DERL QW++GK KE +  D LD+ +  KD +GK  LS +EIKAQ+
Sbjct: 280 DERLMQWRNGKMKEPQ--DFLDMFIIAKDTDGKPTLSDEEIKAQV 320

[Close]

BLAST of Lsi06G005580 vs. ExPASy Swiss-Prot
Match: Q9M7B7 (Valine N-monooxygenase 2 OS=Manihot esculenta OX=3983 GN=CYP79D2 PE=1 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 8.3e-76
Identity = 145/325 (44.62%), Postives = 221/325 (68.00%), Query Frame = 0


Query: 8   SPSSYQNSAKAAIAITFIAIILFISSIIYVIRFSQYYRKRTPSFPLLPPGPKPWPLLGCL 67
           S SS  N+AK  +   FI+I+    S +  ++    Y+K + +FP LPPGP PWPL+G +
Sbjct: 17  STSSMNNTAKILLITLFISIV----STVIKLQKRASYKKASKNFP-LPPGPTPWPLIGNI 76

Query: 68  PAMLSNKDLPTHQWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFLNTHDSVFGSR 127
           P M+  +  PT +WIH +MK+ NT+I  IR G  T+++P++ P +A E L  HD+VF +R
Sbjct: 77  PEMIRYR--PTFRWIHQLMKDMNTDICLIRFG-KTNVVPISCPVIAREILKKHDAVFSNR 136

Query: 128 PISMTAEILSRGYLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRTKEADTLLRYI 187
           P  + A+ +S GYL+T++ P  DQWKKMR++L S++++P+    +  +R +EAD L+ YI
Sbjct: 137 PKILCAKTMSGGYLTTIVVPYNDQWKKMRKVLTSEIISPARHKWLHDKRAEEADQLVFYI 196

Query: 188 FILTRNGEAVDIRSITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGHNQALLTILKH 247
               ++ + V++R   +HY GN+IR+MMF++RY+GKG  DGGP  EE  H  A+ T LK+
Sbjct: 197 NNQYKSNKNVNVRIAARHYGGNVIRKMMFSKRYFGKGMPDGGPGPEEIMHVDAIFTALKY 256

Query: 248 VNAFSISDFIPFLKPLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDGKKKEVEEDDV 307
           +  F ISD++PFL+ LDLDG  KI+  A   IR   +P+I+ER+QQW+ G++KE+E  D+
Sbjct: 257 LYGFCISDYLPFLEGLDLDGQEKIVLNANKTIRDLQNPLIEERIQQWRSGERKEME--DL 316

Query: 308 LDILLSLKDENGKSLLSIDEIKAQI 333
           LD+ ++L+D +GK LL+ DEIK QI
Sbjct: 317 LDVFITLQDSDGKPLLNPDEIKNQI 331

[Close]

BLAST of Lsi06G005580 vs. ExPASy TrEMBL
Match: A0A0A0KM68 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G223620 PE=4 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 4.2e-139
Identity = 246/315 (78.10%), Postives = 283/315 (89.84%), Query Frame = 0


Query: 20  IAITFIAIILFISSIIYVIRFSQYYRKRTPSFPLLPPGPKPWPLLGCLPAMLSNKDLPTH 79
           +AITF+AIILFI SIIY I+FSQYY K TPS   LPPGPKPWPL+GCLPAMLSNK+LP +
Sbjct: 1   MAITFLAIILFIYSIIYAIKFSQYYLKGTPSIRRLPPGPKPWPLIGCLPAMLSNKNLPAY 60

Query: 80  QWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFLNTHDSVFGSRPISMTAEILSRG 139
           QWIH +MK+FNTEIACIRLG NTHIIPV SPEL+LEFLNTHDSVFGSR ISMTAEI+S G
Sbjct: 61  QWIHEVMKQFNTEIACIRLGSNTHIIPVASPELSLEFLNTHDSVFGSRSISMTAEIVSNG 120

Query: 140 YLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRTKEADTLLRYIFILTRNGEAVDI 199
           YLSTVLSPMG+QWKKMR+IL SQVLN STLHRMLGQRT EAD LLRYIF LT+NGEA++I
Sbjct: 121 YLSTVLSPMGEQWKKMRKILASQVLNSSTLHRMLGQRTDEADILLRYIFGLTKNGEAINI 180

Query: 200 RSITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGHNQALLTILKHVNAFSISDFIPF 259
           RSI +HYCG +IRRM+F+RRYYGKGREDGGP+ EEE HNQALL+IL+HVNAFSIS+FIP 
Sbjct: 181 RSIVRHYCGTVIRRMIFSRRYYGKGREDGGPSLEEEEHNQALLSILRHVNAFSISEFIPL 240

Query: 260 LKPLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDGKKKEVEEDDVLDILLSLKDENG 319
           LK  DLDGHGKI+++AL  IR +D+PII+ER+Q+W DGKKK+VE  D+LDIL+SLK+ENG
Sbjct: 241 LKTFDLDGHGKIVKRALKVIRNHDEPIIEERVQEWGDGKKKKVE--DILDILISLKNENG 300

Query: 320 KSLLSIDEIKAQIMV 335
           KSLLSI+EIKAQ+ V
Sbjct: 301 KSLLSIEEIKAQVTV 313

[Close]

BLAST of Lsi06G005580 vs. ExPASy TrEMBL
Match: A0A5A7VKT9 (Isoleucine N-monooxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold14G00680 PE=3 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 2.3e-137
Identity = 242/313 (77.32%), Postives = 279/313 (89.14%), Query Frame = 0


Query: 20  IAITFIAIILFISSIIYVIRFSQYYRKRTPSFPLLPPGPKPWPLLGCLPAMLSNKDLPTH 79
           +AITFIAIILFI SI+Y I+FSQYY   TPS   LPPGPKPWPL+GCLPAML NK+LPT+
Sbjct: 1   MAITFIAIILFIFSILYAIKFSQYYHNGTPSIRRLPPGPKPWPLIGCLPAMLRNKNLPTY 60

Query: 80  QWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFLNTHDSVFGSRPISMTAEILSRG 139
           QWIH +MK+FNTEIACIRLG NTHIIPV SPEL+LEFLNTHDSVFGSR ISMTAEI+S G
Sbjct: 61  QWIHEVMKQFNTEIACIRLGSNTHIIPVASPELSLEFLNTHDSVFGSRSISMTAEIVSNG 120

Query: 140 YLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRTKEADTLLRYIFILTRNGEAVDI 199
           YLSTVLSPMG+QWKKMR+ILVSQVLNP TLH+M+GQRT EAD LLRYIF LTRNGEA++I
Sbjct: 121 YLSTVLSPMGEQWKKMRKILVSQVLNPPTLHQMVGQRTDEADILLRYIFGLTRNGEAINI 180

Query: 200 RSITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGHNQALLTILKHVNAFSISDFIPF 259
           RSI +HYCG +IRRM+F+RRYYGKGREDGGP+ EEE HNQALL+IL+HVNAFSIS+FIP 
Sbjct: 181 RSIVRHYCGTVIRRMIFSRRYYGKGREDGGPSLEEEDHNQALLSILRHVNAFSISEFIPL 240

Query: 260 LKPLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDGKKKEVEEDDVLDILLSLKDENG 319
           LK  DLDGH KI+++AL  IR  D+PII+ER+Q W+DGKKK+ E  D+LDIL+SLK+ENG
Sbjct: 241 LKKFDLDGHEKIVKRALKVIRNNDEPIIEERVQDWRDGKKKKAE--DILDILISLKNENG 300

Query: 320 KSLLSIDEIKAQI 333
           KSLLSI+EIKAQ+
Sbjct: 301 KSLLSIEEIKAQV 311

[Close]

BLAST of Lsi06G005580 vs. ExPASy TrEMBL
Match: A0A1S3BNA6 (phenylalanine N-monooxygenase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491937 PE=3 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 3.3e-120
Identity = 229/335 (68.36%), Postives = 267/335 (79.70%), Query Frame = 0


Query: 1   MKALLLISPSSYQNSAKAAIAITFIAIILFISS-IIYVIRFSQYYRKRTPSFPLLPPGPK 60
           MKALL  S  + QN  K AI  TFI+I+LFISS I+Y+IR SQYY KR P  P LPPGP+
Sbjct: 1   MKALLFNSQLACQNFEKPAIPKTFISILLFISSLIVYLIRISQYYNKRNPLEPRLPPGPR 60

Query: 61  PWPLLG-CLPAMLSN-KDLPTHQWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFL 120
           PWPL G CLP MLS+ K  P HQWIH++MK+ NTEIAC  LG NTHII VTSPELALEFL
Sbjct: 61  PWPLFGSCLPLMLSSYKRSPRHQWIHTVMKQLNTEIACFHLGSNTHIIIVTSPELALEFL 120

Query: 121 NTHDSVFGSRPISMTAEILSRGYLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRT 180
            THDS F SRPI+ T EILS G+LSTVLSPMGDQWKKM+RIL S VLNPSTLH+M GQR 
Sbjct: 121 KTHDSTFASRPIASTTEILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRI 180

Query: 181 KEADTLLRYIFILTRNGEAVDIRSITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGH 240
           KEAD LL YIF      EA++IRSITQHYC NIIRRM+FNRRYY KGRE+ GP+ EEE +
Sbjct: 181 KEADALLHYIFDHASRCEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDY 240

Query: 241 NQALLTILKHVNAFSISDFIPFLKPLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDG 300
           NQALLTIL H+ +FSIS++I  LKP DL GH KI+ KAL  I  +D PII+ER+Q+W+DG
Sbjct: 241 NQALLTILMHLYSFSISEYIACLKPFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDG 300

Query: 301 KKKEVEEDDVLDILLSLKDENGKSLLSIDEIKAQI 333
           KKKEV+  D+LDIL+++K ENG+SLLSI+EIKAQ+
Sbjct: 301 KKKEVQ--DILDILITVKKENGESLLSIEEIKAQV 333

[Close]

BLAST of Lsi06G005580 vs. ExPASy TrEMBL
Match: A0A1S3BPW4 (phenylalanine N-monooxygenase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491937 PE=3 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 3.3e-120
Identity = 229/335 (68.36%), Postives = 267/335 (79.70%), Query Frame = 0


Query: 1   MKALLLISPSSYQNSAKAAIAITFIAIILFISS-IIYVIRFSQYYRKRTPSFPLLPPGPK 60
           MKALL  S  + QN  K AI  TFI+I+LFISS I+Y+IR SQYY KR P  P LPPGP+
Sbjct: 1   MKALLFNSQLACQNFEKPAIPKTFISILLFISSLIVYLIRISQYYNKRNPLEPRLPPGPR 60

Query: 61  PWPLLG-CLPAMLSN-KDLPTHQWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFL 120
           PWPL G CLP MLS+ K  P HQWIH++MK+ NTEIAC  LG NTHII VTSPELALEFL
Sbjct: 61  PWPLFGSCLPLMLSSYKRSPRHQWIHTVMKQLNTEIACFHLGSNTHIIIVTSPELALEFL 120

Query: 121 NTHDSVFGSRPISMTAEILSRGYLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRT 180
            THDS F SRPI+ T EILS G+LSTVLSPMGDQWKKM+RIL S VLNPSTLH+M GQR 
Sbjct: 121 KTHDSTFASRPIASTTEILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRI 180

Query: 181 KEADTLLRYIFILTRNGEAVDIRSITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGH 240
           KEAD LL YIF      EA++IRSITQHYC NIIRRM+FNRRYY KGRE+ GP+ EEE +
Sbjct: 181 KEADALLHYIFDHASRCEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDY 240

Query: 241 NQALLTILKHVNAFSISDFIPFLKPLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDG 300
           NQALLTIL H+ +FSIS++I  LKP DL GH KI+ KAL  I  +D PII+ER+Q+W+DG
Sbjct: 241 NQALLTILMHLYSFSISEYIACLKPFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDG 300

Query: 301 KKKEVEEDDVLDILLSLKDENGKSLLSIDEIKAQI 333
           KKKEV+  D+LDIL+++K ENG+SLLSI+EIKAQ+
Sbjct: 301 KKKEVQ--DILDILITVKKENGESLLSIEEIKAQV 333

[Close]

BLAST of Lsi06G005580 vs. ExPASy TrEMBL
Match: A0A6J1D6G9 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498 PE=3 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 2.0e-117
Identity = 224/340 (65.88%), Postives = 269/340 (79.12%), Query Frame = 0


Query: 1   MKALLLISPSSYQNSAKAAI-AITFIAIILFISSIIYVIRFSQYYRKRTPSFPLLPPGPK 60
           M+ALL IS  +YQNS  A     T    + FIS+I     FSQ YRKR  S   LPPGP 
Sbjct: 1   MEALLFISQLTYQNSPTAITKTFTLFVFMAFISAI-----FSQCYRKRKHSVLQLPPGPN 60

Query: 61  PWPLLGCLPAMLSNKDLPTHQWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFLNT 120
           PWPL+GCLPAML+ K  P ++WIH++MK+F+TEIACIRLG NT+++PVTSPELA+EFL  
Sbjct: 61  PWPLVGCLPAMLATKS-PKYKWIHAVMKQFDTEIACIRLG-NTYVMPVTSPELAVEFLKR 120

Query: 121 HDSVFGSR-PISMTAEILSRGYLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRTK 180
           HDSVF SR  IS T  +LSRG L+T  SP+G QWKKMRRIL S++  PSTLH+MLG RT 
Sbjct: 121 HDSVFASRSTISNTVNVLSRGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRTN 180

Query: 181 EADTLLRYIFILT------RNGEAVDIRSITQHYCGNIIRRMMFNRRYYGKGREDGGPTF 240
           EADTLLRYIF LT      ++G  V++RS+TQHYCGNIIRRM+FN+RYYGKGREDGGP+F
Sbjct: 181 EADTLLRYIFSLTTTNTVNKDGAVVNVRSVTQHYCGNIIRRMLFNKRYYGKGREDGGPSF 240

Query: 241 EEEGHNQALLTILKHVNAFSISDFIPFLKPLDLDGHGKILEKALNFIRRYDDPIIDERLQ 300
           EEE HNQALLTIL+HVNAFSISDF+P LKP DLDGH KI+++AL  + +YD+PII+ER+Q
Sbjct: 241 EEEEHNQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKRALKALWKYDEPIINERVQ 300

Query: 301 QWKDGKKKEVEEDDVLDILLSLKDENGKSLLSIDEIKAQI 333
           QWKDGK++  E  D+LDIL+S KD NGKSLL+IDEIKAQI
Sbjct: 301 QWKDGKREGAE--DILDILISTKDGNGKSLLTIDEIKAQI 331

[Close]

BLAST of Lsi06G005580 vs. NCBI nr
Match: XP_011655052.1 (isoleucine N-monooxygenase 1 [Cucumis sativus] >KAE8648059.1 hypothetical protein Csa_005885 [Cucumis sativus])

HSP 1 Score: 503.4 bits (1295), Expect = 1.5e-138
Identity = 245/313 (78.27%), Postives = 282/313 (90.10%), Query Frame = 0


Query: 20  IAITFIAIILFISSIIYVIRFSQYYRKRTPSFPLLPPGPKPWPLLGCLPAMLSNKDLPTH 79
           +AITF+AIILFI SIIY I+FSQYY K TPS   LPPGPKPWPL+GCLPAMLSNK+LP +
Sbjct: 1   MAITFLAIILFIYSIIYAIKFSQYYLKGTPSIRRLPPGPKPWPLIGCLPAMLSNKNLPAY 60

Query: 80  QWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFLNTHDSVFGSRPISMTAEILSRG 139
           QWIH +MK+FNTEIACIRLG NTHIIPV SPEL+LEFLNTHDSVFGSR ISMTAEI+S G
Sbjct: 61  QWIHEVMKQFNTEIACIRLGSNTHIIPVASPELSLEFLNTHDSVFGSRSISMTAEIVSNG 120

Query: 140 YLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRTKEADTLLRYIFILTRNGEAVDI 199
           YLSTVLSPMG+QWKKMR+IL SQVLN STLHRMLGQRT EAD LLRYIF LT+NGEA++I
Sbjct: 121 YLSTVLSPMGEQWKKMRKILASQVLNSSTLHRMLGQRTDEADILLRYIFGLTKNGEAINI 180

Query: 200 RSITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGHNQALLTILKHVNAFSISDFIPF 259
           RSI +HYCG +IRRM+F+RRYYGKGREDGGP+ EEE HNQALL+IL+HVNAFSIS+FIP 
Sbjct: 181 RSIVRHYCGTVIRRMIFSRRYYGKGREDGGPSLEEEEHNQALLSILRHVNAFSISEFIPL 240

Query: 260 LKPLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDGKKKEVEEDDVLDILLSLKDENG 319
           LK  DLDGHGKI+++AL  IR +D+PII+ER+Q+W DGKKK+VE  D+LDIL+SLK+ENG
Sbjct: 241 LKTFDLDGHGKIVKRALKVIRNHDEPIIEERVQEWGDGKKKKVE--DILDILISLKNENG 300

Query: 320 KSLLSIDEIKAQI 333
           KSLLSI+EIKAQ+
Sbjct: 301 KSLLSIEEIKAQV 311

[Close]

BLAST of Lsi06G005580 vs. NCBI nr
Match: KAA0067056.1 (isoleucine N-monooxygenase 1 [Cucumis melo var. makuwa] >TYK26293.1 isoleucine N-monooxygenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 498.4 bits (1282), Expect = 4.7e-137
Identity = 242/313 (77.32%), Postives = 279/313 (89.14%), Query Frame = 0


Query: 20  IAITFIAIILFISSIIYVIRFSQYYRKRTPSFPLLPPGPKPWPLLGCLPAMLSNKDLPTH 79
           +AITFIAIILFI SI+Y I+FSQYY   TPS   LPPGPKPWPL+GCLPAML NK+LPT+
Sbjct: 1   MAITFIAIILFIFSILYAIKFSQYYHNGTPSIRRLPPGPKPWPLIGCLPAMLRNKNLPTY 60

Query: 80  QWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFLNTHDSVFGSRPISMTAEILSRG 139
           QWIH +MK+FNTEIACIRLG NTHIIPV SPEL+LEFLNTHDSVFGSR ISMTAEI+S G
Sbjct: 61  QWIHEVMKQFNTEIACIRLGSNTHIIPVASPELSLEFLNTHDSVFGSRSISMTAEIVSNG 120

Query: 140 YLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRTKEADTLLRYIFILTRNGEAVDI 199
           YLSTVLSPMG+QWKKMR+ILVSQVLNP TLH+M+GQRT EAD LLRYIF LTRNGEA++I
Sbjct: 121 YLSTVLSPMGEQWKKMRKILVSQVLNPPTLHQMVGQRTDEADILLRYIFGLTRNGEAINI 180

Query: 200 RSITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGHNQALLTILKHVNAFSISDFIPF 259
           RSI +HYCG +IRRM+F+RRYYGKGREDGGP+ EEE HNQALL+IL+HVNAFSIS+FIP 
Sbjct: 181 RSIVRHYCGTVIRRMIFSRRYYGKGREDGGPSLEEEDHNQALLSILRHVNAFSISEFIPL 240

Query: 260 LKPLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDGKKKEVEEDDVLDILLSLKDENG 319
           LK  DLDGH KI+++AL  IR  D+PII+ER+Q W+DGKKK+ E  D+LDIL+SLK+ENG
Sbjct: 241 LKKFDLDGHEKIVKRALKVIRNNDEPIIEERVQDWRDGKKKKAE--DILDILISLKNENG 300

Query: 320 KSLLSIDEIKAQI 333
           KSLLSI+EIKAQ+
Sbjct: 301 KSLLSIEEIKAQV 311

[Close]

BLAST of Lsi06G005580 vs. NCBI nr
Match: XP_038877584.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 467.6 bits (1202), Expect = 9.0e-128
Identity = 240/339 (70.80%), Postives = 278/339 (82.01%), Query Frame = 0


Query: 1   MKALLLISPSSYQNSAKAAIAITFIAIILFISS-IIYVIRFSQYYRKRTPSFPLLPPGPK 60
           MKA L  S  +YQN  KAAIA TFI+ ILFISS IIYVIR   YY KR P    LPPGPK
Sbjct: 1   MKAFLFSSELAYQNFGKAAIAKTFISFILFISSFIIYVIRIYHYYHKRKPLALRLPPGPK 60

Query: 61  PWPLLGCLPAM-LSNKDLPTHQWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFLN 120
           PWPLLGCLPAM L  K+ PTHQWIH +MK+FN EIACIRLG NTHIIPV SPELALEFL 
Sbjct: 61  PWPLLGCLPAMLLRKKNSPTHQWIHEVMKQFNNEIACIRLGSNTHIIPVASPELALEFLK 120

Query: 121 THDSVFGSRP-ISMTAEILSRGYLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRT 180
           T+DSVF SRP IS+TA++LS G+L TV+ P+GDQWKKMR+ L S V NPSTLHR+LGQR 
Sbjct: 121 TYDSVFASRPIISITAQMLSYGFLGTVVLPIGDQWKKMRKNLASHVFNPSTLHRILGQRL 180

Query: 181 KEADTLLRYIFILTRNGEAVDIRSITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGH 240
           K+ADTLL Y+F LTR+ EA++IRSITQHYC NIIRRM+FNRRYYGKGRED GP+FEEE H
Sbjct: 181 KKADTLLCYVFSLTRSCEAINIRSITQHYCNNIIRRMIFNRRYYGKGREDEGPSFEEEYH 240

Query: 241 NQALLTILKHVNAFSISDFIPFLKPLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDG 300
           NQALLTIL H  AFS+S+F+P LKP DLDGH KI++ ALN IR YD+PII+E +Q+W+DG
Sbjct: 241 NQALLTILMHTYAFSVSEFMPCLKPFDLDGHRKIVKGALNVIRNYDEPIIEESVQEWRDG 300

Query: 301 KKKEVEEDDVLDILLSLKDENGKSLLSIDEIKAQIMVSI 337
           ++ EVE  D+LDIL+SLK+ENG S+LSI+EI AQI V I
Sbjct: 301 RRNEVE--DILDILISLKNENGTSMLSIEEISAQITVGI 337

[Close]

BLAST of Lsi06G005580 vs. NCBI nr
Match: XP_008450287.1 (PREDICTED: phenylalanine N-monooxygenase-like isoform X1 [Cucumis melo])

HSP 1 Score: 441.4 bits (1134), Expect = 6.9e-120
Identity = 229/335 (68.36%), Postives = 267/335 (79.70%), Query Frame = 0


Query: 1   MKALLLISPSSYQNSAKAAIAITFIAIILFISS-IIYVIRFSQYYRKRTPSFPLLPPGPK 60
           MKALL  S  + QN  K AI  TFI+I+LFISS I+Y+IR SQYY KR P  P LPPGP+
Sbjct: 1   MKALLFNSQLACQNFEKPAIPKTFISILLFISSLIVYLIRISQYYNKRNPLEPRLPPGPR 60

Query: 61  PWPLLG-CLPAMLSN-KDLPTHQWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFL 120
           PWPL G CLP MLS+ K  P HQWIH++MK+ NTEIAC  LG NTHII VTSPELALEFL
Sbjct: 61  PWPLFGSCLPLMLSSYKRSPRHQWIHTVMKQLNTEIACFHLGSNTHIIIVTSPELALEFL 120

Query: 121 NTHDSVFGSRPISMTAEILSRGYLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRT 180
            THDS F SRPI+ T EILS G+LSTVLSPMGDQWKKM+RIL S VLNPSTLH+M GQR 
Sbjct: 121 KTHDSTFASRPIASTTEILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRI 180

Query: 181 KEADTLLRYIFILTRNGEAVDIRSITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGH 240
           KEAD LL YIF      EA++IRSITQHYC NIIRRM+FNRRYY KGRE+ GP+ EEE +
Sbjct: 181 KEADALLHYIFDHASRCEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDY 240

Query: 241 NQALLTILKHVNAFSISDFIPFLKPLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDG 300
           NQALLTIL H+ +FSIS++I  LKP DL GH KI+ KAL  I  +D PII+ER+Q+W+DG
Sbjct: 241 NQALLTILMHLYSFSISEYIACLKPFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDG 300

Query: 301 KKKEVEEDDVLDILLSLKDENGKSLLSIDEIKAQI 333
           KKKEV+  D+LDIL+++K ENG+SLLSI+EIKAQ+
Sbjct: 301 KKKEVQ--DILDILITVKKENGESLLSIEEIKAQV 333

[Close]

BLAST of Lsi06G005580 vs. NCBI nr
Match: XP_008450288.1 (PREDICTED: phenylalanine N-monooxygenase-like isoform X2 [Cucumis melo])

HSP 1 Score: 441.4 bits (1134), Expect = 6.9e-120
Identity = 229/335 (68.36%), Postives = 267/335 (79.70%), Query Frame = 0


Query: 1   MKALLLISPSSYQNSAKAAIAITFIAIILFISS-IIYVIRFSQYYRKRTPSFPLLPPGPK 60
           MKALL  S  + QN  K AI  TFI+I+LFISS I+Y+IR SQYY KR P  P LPPGP+
Sbjct: 1   MKALLFNSQLACQNFEKPAIPKTFISILLFISSLIVYLIRISQYYNKRNPLEPRLPPGPR 60

Query: 61  PWPLLG-CLPAMLSN-KDLPTHQWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFL 120
           PWPL G CLP MLS+ K  P HQWIH++MK+ NTEIAC  LG NTHII VTSPELALEFL
Sbjct: 61  PWPLFGSCLPLMLSSYKRSPRHQWIHTVMKQLNTEIACFHLGSNTHIIIVTSPELALEFL 120

Query: 121 NTHDSVFGSRPISMTAEILSRGYLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRT 180
            THDS F SRPI+ T EILS G+LSTVLSPMGDQWKKM+RIL S VLNPSTLH+M GQR 
Sbjct: 121 KTHDSTFASRPIASTTEILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRI 180

Query: 181 KEADTLLRYIFILTRNGEAVDIRSITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGH 240
           KEAD LL YIF      EA++IRSITQHYC NIIRRM+FNRRYY KGRE+ GP+ EEE +
Sbjct: 181 KEADALLHYIFDHASRCEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDY 240

Query: 241 NQALLTILKHVNAFSISDFIPFLKPLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDG 300
           NQALLTIL H+ +FSIS++I  LKP DL GH KI+ KAL  I  +D PII+ER+Q+W+DG
Sbjct: 241 NQALLTILMHLYSFSISEYIACLKPFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDG 300

Query: 301 KKKEVEEDDVLDILLSLKDENGKSLLSIDEIKAQI 333
           KKKEV+  D+LDIL+++K ENG+SLLSI+EIKAQ+
Sbjct: 301 KKKEVQ--DILDILITVKKENGESLLSIEEIKAQV 333

[Close]

BLAST of Lsi06G005580 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 288.1 bits (736), Expect = 9.1e-78
Identity = 143/285 (50.18%), Postives = 199/285 (69.82%), Query Frame = 0


Query: 54  LPPGPKPWPLLGCLPAMLSNKDLPTHQWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELA 113
           LPPGPK WPL+G LP +L  ++ P  +WIHS+MKE NT+IACIRL  NTH+IPVTSP +A
Sbjct: 34  LPPGPKSWPLIGNLPEIL-GRNKPVFRWIHSLMKELNTDIACIRLA-NTHVIPVTSPRIA 93

Query: 114 LEFLNTHDSVFGSRPISMTAEILSRGYLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRML 173
            E L   DSVF +RP++M  E  SRGYL+  + P G+QWKKMRR++ S V +  +   ML
Sbjct: 94  REILKKQDSVFATRPLTMGTEYCSRGYLTVAVEPQGEQWKKMRRVVASHVTSKKSFQMML 153

Query: 174 GQRTKEADTLLRYI--FILTRNGEA---VDIRSITQHYCGNIIRRMMFNRRYYGKGREDG 233
            +RT+EAD L+RYI    +   G A   +D+R   + Y GN+ R+MMF  R++GKG EDG
Sbjct: 154 QKRTEEADNLVRYINNRSVKNRGNAFVVIDLRLAVRQYSGNVARKMMFGIRHFGKGSEDG 213

Query: 234 -GPTFEEEGHNQALLTILKHVNAFSISDFIPFLKPLDLDGHGKILEKALNFIRRYDDPII 293
            GP  EE  H ++L T+L H+ AF++SD++P+L+ LDL+GH K++  A+  + +Y+DP +
Sbjct: 214 SGPGLEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVSNAMRNVSKYNDPFV 273

Query: 294 DERLQQWKDGKKKEVEEDDVLDILLSLKDENGKSLLSIDEIKAQI 333
           DERL QW++GK KE +  D LD+ +  KD +GK  LS +EIKAQ+
Sbjct: 274 DERLMQWRNGKMKEPQ--DFLDMFIIAKDTDGKPTLSDEEIKAQV 314

[Close]

BLAST of Lsi06G005580 vs. TAIR 10
Match: AT5G35917.1 (cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene )

HSP 1 Score: 280.8 bits (717), Expect = 1.4e-75
Identity = 144/287 (50.17%), Postives = 199/287 (69.34%), Query Frame = 0


Query: 54  LPPGPKPWPLLGCLPAMLSNKDLPTHQWIHSIMKEFNTEIACIRLGLNTHIIPVTSPELA 113
           LPPGPK WPL+G LP +L  ++ P  +WIHS+M+E NT+IACIRL  NTH+IPVTSP +A
Sbjct: 42  LPPGPKSWPLVGNLPEIL-GRNKPVFRWIHSLMEELNTDIACIRLA-NTHVIPVTSPRIA 101

Query: 114 LEFLNTHDSVFGSRPISMTAEILSRGYLSTVLSPMGDQWKKMRRILVSQVLNPSTLHRML 173
            E L   DSVF  RP++M  E  SRGYL+  +   G+QWKKMRR++ S V +  +   ML
Sbjct: 102 REILKKQDSVFAIRPLTMGTEYCSRGYLTIAVQSQGEQWKKMRRVVASHVTSKKSFKLML 161

Query: 174 GQRTKEADTLLRYI---FILTR---NGEAV-DIRSITQHYCGNIIRRMMFNRRYYGKGRE 233
            +RT+EAD L+RYI    +  R   NG AV D+R + + Y GN+ R+MMF  R++GKG E
Sbjct: 162 EKRTEEADNLVRYINNRCVKNRGNGNGLAVIDLRFVVRQYSGNVARKMMFGIRHFGKGSE 221

Query: 234 D-GGPTFEEEGHNQALLTILKHVNAFSISDFIPFLKPLDLDGHGKILEKALNFIRRYDDP 293
           D  GP  EE  H ++L T+L H+ AF++SD++P+L+ LDL+GH K++  A+  + +Y+DP
Sbjct: 222 DESGPGSEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVADAMRNVSKYNDP 281

Query: 294 IIDERLQQWKDGKKKEVEEDDVLDILLSLKDENGKSLLSIDEIKAQI 333
            +DERL QW++GK KE +  D LD+ +  KD NGK  LS +EIKAQ+
Sbjct: 282 FVDERLMQWRNGKMKEPQ--DFLDMFIMAKDTNGKPTLSEEEIKAQV 324

[Close]

BLAST of Lsi06G005580 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 270.0 bits (689), Expect = 2.6e-72
Identity = 132/283 (46.64%), Postives = 196/283 (69.26%), Query Frame = 0


Query: 52  PLLPPGPKPWPLLGCLPAMLSNKDLPTHQWIHSIMKEFNTEIACIRLGLNTHIIPVTSPE 111
           P LPPGP  WP++G +P ML  K  P  +W+HSIMK+ NTEIAC++LG NTH+I VT P+
Sbjct: 54  PYLPPGPTGWPIIGMIPTML--KSRPVFRWLHSIMKQLNTEIACVKLG-NTHVITVTCPK 113

Query: 112 LALEFLNTHDSVFGSRPISMTAEILSRGYLSTVLSPMGDQWKKMRRILVSQVLNPSTLHR 171
           +A E L   D++F SRP++   +ILS GY + V++P GDQ+KKMR++++++++ P+  HR
Sbjct: 114 IAREILKQQDALFASRPLTYAQKILSNGYKTCVITPFGDQFKKMRKVVMTELVCPAR-HR 173

Query: 172 MLGQ-RTKEADTLLRYIFILTRNGEAVDIRSITQHYCGNIIRRMMFNRRYYGKG-REDGG 231
            L Q R++E D L  +++ + +N  +VD R +T+HYCGN I+++MF  R + K    DGG
Sbjct: 174 WLHQKRSEENDHLTAWVYNMVKNSGSVDFRFMTRHYCGNAIKKLMFGTRTFSKNTAPDGG 233

Query: 232 PTFEEEGHNQALLTILKHVNAFSISDFIPFLKPLDLDGHGKILEKALNFIRRYDDPIIDE 291
           PT E+  H +A+   L    AF ISD++P L  LDL+GH KI+ ++   + +Y DPIIDE
Sbjct: 234 PTVEDVEHMEAMFEALGFTFAFCISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDE 293

Query: 292 RLQQWKDGKKKEVEEDDVLDILLSLKDENGKSLLSIDEIKAQI 333
           R++ W++GK+ ++E  D LDI +S+KDE G  LL+ DEIK  I
Sbjct: 294 RIKMWREGKRTQIE--DFLDIFISIKDEQGNPLLTADEIKPTI 330

[Close]

BLAST of Lsi06G005580 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 269.6 bits (688), Expect = 3.3e-72
Identity = 136/309 (44.01%), Postives = 208/309 (67.31%), Query Frame = 0


Query: 25  IAIILFISSIIYVIRFSQYYRKRTPSFPLLPPGPKPWPLLGCLPAMLSNKDLPTHQWIHS 84
           +A + F+  I+  I+ S   +K  P    LPPGP  +P++G +PAML N+  P  +W+HS
Sbjct: 34  LAALCFL-MILNKIKSSSRNKKLHP----LPPGPTGFPIVGMIPAMLKNR--PVFRWLHS 93

Query: 85  IMKEFNTEIACIRLGLNTHIIPVTSPELALEFLNTHDSVFGSRPISMTAEILSRGYLSTV 144
           +MKE NTEIAC+RLG NTH+IPVT P++A E     D++F SRP++   +ILS GY + V
Sbjct: 94  LMKELNTEIACVRLG-NTHVIPVTCPKIAREIFKQQDALFASRPLTYAQKILSNGYKTCV 153

Query: 145 LSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRTKEADTLLRYIFILTRNGEAVDIRSITQ 204
           ++P G+Q+KKMR++++++++ P+    +   R +E D L  +++ + +N E VD+R +T+
Sbjct: 154 ITPFGEQFKKMRKVIMTEIVCPARHRWLHDNRAEETDHLTAWLYNMVKNSEPVDLRFVTR 213

Query: 205 HYCGNIIRRMMFNRRYYGKGRE-DGGPTFEEEGHNQALLTILKHVNAFSISDFIPFLKPL 264
           HYCGN I+R+MF  R + +  E DGGPT E+  H  A+   L    AF ISD++P L  L
Sbjct: 214 HYCGNAIKRLMFGTRTFSEKTEADGGPTLEDIEHMDAMFEGLGFTFAFCISDYLPMLTGL 273

Query: 265 DLDGHGKILEKALNFIRRYDDPIIDERLQQWKDGKKKEVEEDDVLDILLSLKDENGKSLL 324
           DL+GH KI+ ++   + +Y DPIIDER++ W++GK+ ++E  D LDI +S+KDE G+ LL
Sbjct: 274 DLNGHEKIMRESSAIMDKYHDPIIDERIKMWREGKRTQIE--DFLDIFISIKDEAGQPLL 332

Query: 325 SIDEIKAQI 333
           + DEIK  I
Sbjct: 334 TADEIKPTI 332

[Close]

BLAST of Lsi06G005580 vs. TAIR 10
Match: AT1G58260.1 (cytochrome p450 79c2 )

HSP 1 Score: 196.8 bits (499), Expect = 2.7e-50
Identity = 106/313 (33.87%), Postives = 189/313 (60.38%), Query Frame = 0


Query: 23  TFIAIILFISSIIYVI-RFSQYYRKRTPSFPLLPPGPKPWPLLGCLPAMLSNKDLPTHQW 82
           +F  +++ I+ ++ +  RFS++ + +      LPPGP+ WP++G +  M+ N+  P H W
Sbjct: 9   SFTLVLISITLVLALARRFSRFMKPKGQ----LPPGPRGWPIVGNMLQMIINR--PAHLW 68

Query: 83  IHSIMKEFNTEIACIRLGLNTHIIPVTSPELALEFLNTHDSVFGSRPISMTAEILSRGYL 142
           IH +M+E  T+IAC R     H+I VTS ++A E L   D V   R  S  + ++S GY 
Sbjct: 69  IHRVMEELQTDIACYRFA-RFHVITVTSSKIAREVLREKDEVLADRSESYASHLISHGYK 128

Query: 143 STVLSPMGDQWKKMRRILVSQVLNPSTLHRMLGQRTKEADTLLRYIFILTRNG---EAVD 202
           +   S  G+ WK +++++ +++++P+TL + LG R  EAD ++ Y++ L + G   + ++
Sbjct: 129 NISFSSYGENWKLVKKVMTTKLMSPTTLSKTLGYRNIEADNIVTYVYNLCQLGSVRKPIN 188

Query: 203 IRSITQHYCGNIIRRMMFNRRYYGKGREDGGPTFEEEGHNQALLTILKHVNAFSISDFIP 262
           +R     YC  ++ RMMF +R++ +  E+GG   +E+ H  A+   L    +F+++++IP
Sbjct: 189 VRDTILTYCHAVMMRMMFGQRHFDEVVENGGLGPKEKEHMDAIYLALDCFFSFNLTNYIP 248

Query: 263 FLKPLDLDGHGKILEKALNFIRRYDDPIIDERLQQWKDGKKKEVEEDDVLDILLSLKDEN 322
           FL+  ++D     + +A++ I   +DPII ER+  W+    K++EE D LDIL++LKD+ 
Sbjct: 249 FLRGWNVDKAETEVREAVHIINICNDPIIQERIHLWRKKGGKQMEE-DWLDILITLKDDQ 308

Query: 323 GKSLLSIDEIKAQ 332
           G  L + DEI+AQ
Sbjct: 309 GMHLFTFDEIRAQ 313

The following BLAST results are available for this feature:

BLAST of Lsi06G005580 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q605	4.1e-91	51.29	Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV...	[more]
Q6J541	2.0e-82	50.32	Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1	[more]
Q6J540	1.7e-81	50.48	Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1	[more]
Q9FLC8	1.3e-76	50.18	Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV...	[more]
Q9M7B7	8.3e-76	44.62	Valine N-monooxygenase 2 OS=Manihot esculenta OX=3983 GN=CYP79D2 PE=1 SV=1	[more]

BLAST of Lsi06G005580 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KM68	4.2e-139	78.10	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G223620 PE=4 SV=1	[more]
A0A5A7VKT9	2.3e-137	77.32	Isoleucine N-monooxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3BNA6	3.3e-120	68.36	phenylalanine N-monooxygenase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A1S3BPW4	3.3e-120	68.36	phenylalanine N-monooxygenase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1D6G9	2.0e-117	65.88	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498...	[more]

BLAST of Lsi06G005580 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011655052.1	1.5e-138	78.27	isoleucine N-monooxygenase 1 [Cucumis sativus] >KAE8648059.1 hypothetical protei...	[more]
KAA0067056.1	4.7e-137	77.32	isoleucine N-monooxygenase 1 [Cucumis melo var. makuwa] >TYK26293.1 isoleucine N...	[more]
XP_038877584.1	9.0e-128	70.80	tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida]	[more]
XP_008450287.1	6.9e-120	68.36	PREDICTED: phenylalanine N-monooxygenase-like isoform X1 [Cucumis melo]	[more]
XP_008450288.1	6.9e-120	68.36	PREDICTED: phenylalanine N-monooxygenase-like isoform X2 [Cucumis melo]	[more]

BLAST of Lsi06G005580 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05260.1	9.1e-78	50.18	cytochrome p450 79a2	[more]
AT5G35917.1	1.4e-75	50.17	cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene	[more]
AT4G39950.1	2.6e-72	46.64	cytochrome P450, family 79, subfamily B, polypeptide 2	[more]
AT2G22330.1	3.3e-72	44.01	cytochrome P450, family 79, subfamily B, polypeptide 3	[more]
AT1G58260.1	2.7e-50	33.87	cytochrome p450 79c2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 55..335 e-value: 1.3E-22 score: 80.2
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 40..337 e-value: 1.1E-40 score: 141.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 49..336
None	No IPR available	PANTHER	PTHR24298:SF675	PHENYLALANINE N-MONOOXYGENASE	coord: 28..333
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 28..333

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G005580.1	Lsi06G005580.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen